| [effect of immunization on the in vivo immunocytes reaction to foreign particles in the larvae of the flesh fly calliphora vomitoria]. | bacterial immunization of calliphora vomitoria larvae induces hemocytic defense reaction in response to abiotic foreign particles injections. this reaction depends on the larval age and, consequently, the immunocytes composition. the juvenile plasmatocytes which are abundantly present in the end feeding and crop emptying larvae are initially very active and their reaction rate does not depend on the immunization. the plasmatocytes i appear after crop emptying. immunization has a positive effect ... | 2010 | 20737902 |
| a new laboratory cultivation of paramecium bursaria using non-pathogenic bacteria strains. | in most studies dealing with the laboratory cultivation of paramecia (paramecium bursaria), klebsiella pneumoniae bacteria are used to inoculate the medium. however, klebsiella pneumoniae is a typical pathogen, and its use is always associated with a risk of infection. the aim of the present research was to examine non-pathogenic bacteria strains as components of the medium for paramecium bursaria. the paramecia were incubated on lettuce infusions bacterized with different bacteria strains: baci ... | 2010 | 20737917 |
| antimicrobial and anticancer efficacy of antineoplastic agent capped gold nanoparticles. | synthesis of thioguanine (tg)-capped au nanoparticles (au@tg) and their enhanced in vitro antimicrobial and anticancer efficacy against micrococcus luteus, staphylococcus aureus, pseudomonas aeruginosa, e. coli, aspergillus fumigatus, aspergillus niger and hep2 cancer cell (human epidermiod cell) have been reported. the nature of binding between 6-tg and the gold nanoparticles via complexation is investigated using ultraviolet-visible spectrum, cyclic voltammetry, transmission electron microscop ... | 2010 | 20738066 |
| the micrococcus of pneumonia. | | 1884 | 20751086 |
| the "micrococcus rheumaticus": its cultural and other characters. | | 1904 | 20761796 |
| a case of acute endocarditis due to the micrococcus gonorrhoeae. | | 1905 | 20761980 |
| production and characterization of monoclonal antibody against mycobacterium tuberculosis rpfb domain. | rpfb, one of the five resuscitation-promoting factors (rpfs) produced by mycobacterium tuberculosis (mtb), plays an important role in the resuscitation and growth of the dormant mtb. rpfb is likely the target antigen recognized by the host immune system. studies have shown that rpf genes exist in many bacteria and their encoded proteins all contain rpf-like domain. it is likely that this domain has biological characteristics and immunogenicity similar to that of the complete rpf protein. therefo ... | 2010 | 20795307 |
| adaptor protein is essential for insect cytokine signaling in hemocytes. | growth-blocking peptide (gbp) is an insect cytokine that stimulates a class of immune cells called plasmatocytes to adhere to one another and to foreign surfaces. although extensive structure-activity studies have been performed on the gbp and its mutants in lepidoptera pseudaletia separata, the signaling pathway of gbp-dependent activation of plasmatocytes remains unknown. we identified an adaptor protein (p77) with a molecular mass of 77 kda containing sh2/sh3 domain binding motifs and an immu ... | 2010 | 20798052 |
| biosynthetic pathway for γ-cyclic sarcinaxanthin in micrococcus luteus: heterologous expression and evidence for diverse and multiple catalytic functions of c(50) carotenoid cyclases. | we report the cloning and characterization of the biosynthetic gene cluster (crte, crtb, crti, crte2, crtyg, crtyh, and crtx) of the γ-cyclic c(50) carotenoid sarcinaxanthin in micrococcus luteus nctc2665. expression of the complete and partial gene cluster in escherichia coli hosts revealed that sarcinaxanthin biosynthesis from the precursor molecule farnesyl pyrophosphate (fpp) proceeds via c(40) lycopene, c(45) nonaflavuxanthin, c(50) flavuxanthin, and c(50) sarcinaxanthin. glucosylation of s ... | 2010 | 20802040 |
| effect of enzyme modification by well-defined multi-armed poly(ethylene glycol) synthesized using polyamidoamine dendron. | egg white lysozyme was chemically modified by pegylated pamam 1st, 2nd and 3rd generation dendrons, which had 2, 4 and 8 peg arms, respectively. the number of peg chains introduced to the lysozyme molecule drastically increased with an increase in dendron generation, although the number of pegylated pamam dendrons introduced decreased due to steric repulsion. the lytic activity of lysozyme to micrococcus luteus cells was effectively inhibited by conjugating pegylated pamam dendron to the lysozym ... | 2012 | 20810022 |
| rapid differentiation of staphylococcus aureus, staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growth-positive blood cultures by multiplex real-time pcr. | this study evaluated a multiplex real-time pcr method specific for the meca, fema-sa and fema-se genes for rapid identification of staphylococcus aureus, staphylococcus epidermidis and non-s. epidermidis coagulase-negative staphylococci (cons), and meticillin susceptibility testing directly in positive blood cultures that grew gram-positive cocci in clusters. a total of 100 positive blood cultures produced: 39 s. aureus [12 meticillin-resistant s. aureus (mrsa), 31% of all the s. aureus]; 30 s. ... | 2010 | 20813851 |
| detection of bacterial and viral organisms from the conjunctiva of cats with conjunctivitis and upper respiratory tract disease. | a variety of pathogens are involved in conjunctivitis in cats. in this study, the prevalence of feline herpesvirus (fhv), chlamydophila felis, mycoplasmas, and aerobic bacteria on the conjunctival surface of cats with conjunctivitis and upper respiratory tract disease was investigated by polymerase chain reaction (pcr), immunofluorescent assay (ifa), and aerobic bacterial culture of ocular swabs. forty-one cats were included of which 37 were found to be infected with an ocular organism. single a ... | 2010 | 20817584 |
| nitrite pickling salt as an alternative to formaldehyde for embalming in veterinary anatomy--a study based on histo- and microbiological analyses. | formaldehyde, the traditional embalming agent has negative health effects. nitrite pickling salt has been reported to be a good and inexpensive alternative when supplemented with antioxidants, but the antioxidants caused yellowish colouration of cadavers, and damaged corrosion-resistant steel tables and stone floors. here, nitrite pickling salt was supplemented with ethanol and pluriol(®) and tested for effectiveness as an embalming agent of twenty dog cadavers: 10 with open, and 10 with closed ... | 2011 | 20829010 |
| study of microbial contamination and dosing accuracy of oral dispensers. | the optimal administration of liquid medications requires accurate dose delivery. this is particularly important in the treatment of infants and children, as well as elderly people, who are more sensitive to dosage errors. previous studies revealed significant dosage inaccuracies with measuring spoons. oral syringes are therefore generally recommended instead. there is no data on the efficacy of standard cleaning techniques, and consequently on the degree of microbial contamination associated wi ... | 2010 | 20831530 |
| phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal. | the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ... | 2010 | 20833430 |
| screening of the constituents, antimicrobial and antioxidant activity of endemic origanum hypericifolium o. schwartz & p.h. davis. | the chemical compositions, total phenol content, antioxidant and antimicrobial activities with oxidant status of the essential oil from an endemic turkish species, origanum hypericifolium, were investigated. steam distillation (sd) was used to isolate the essential oils, and the chemical analyses were performed by gas chromatography-mass spectrometry (gc-ms). the antimicrobial activity was tested by agar disc diffusion method against morganella morganii (clinic isolate), micrococcus flavus (clin ... | 2010 | 20835958 |
| elimination of erythromycin in milk after intramammary administration in cows with specific mastitis: relation to dose, milking frequency and udder health. | elimination of erythromycin in milk following intramammary therapy of specific mastitis in cows was studied. five cows received therapy in one quarter (g1), and eight in two quarters with five milked twice (g2) and three thrice a day (g3). dose infused was 300 mg/quarter 12 h × 5 times. the drug concentrations in milk were determined using microbial assay technique with micrococcus luteus as the test organism. considerable variations occurred in the excretion of drug; levels for treated quarters ... | 2011 | 20844949 |
| assessment of euphorbia hirta l. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. | the antimicrobial activities of the methanolic extracts of euphorbia hirta l leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. four gram positive (staphylococcus aureus, micrococcus sp., bacillus subtilis and bacillus thuringensis), four gram negative (escherichia coli, klebsiella pneumonia, salmonella typhi and p. mirabilis) and one yeast (candida albicans) species were screened. inhibition zones ranged betw ... | 2010 | 20877206 |
| molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain. | antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ... | 2010 | 20883796 |
| a well protected intruder: the effective antimicrobial defense of the invasive ladybird harmonia axyridis. | the harlequin ladybird harmonia axyridis (coleoptera: coccinellidae) is a polyphagous predatory beetle native to central and eastern asia. since 2007 it has established all over central europe. in order to elucidate which defense strategy is responsible for its high resistance to diseases, we tested hemolymph as well as eleven main components of the headspace of h. axyridis for antimicrobial activity against gram-positive (bacillus subtilis, b. thuringiensis ssp. tenebrionis, micrococcus luteus) ... | 2010 | 20890794 |
| characterization and immune function of two intracellular sensors, hmtlr1 and hmnlr, in the injured cns of an invertebrate. | unlike mammals, the cns of the medicinal leech can regenerate damaged neurites, thus restoring neural functions. our group recently demonstrated that the injured leech nerve cord is able to mount an immune response, which promotes the regenerative processes. this defense mechanism is microorganism-specific, suggesting that the leech cns is able to discriminate among microbial components. we report here the characterization of two receptors potentially implicated in this detection: hmtlr1 and hmn ... | 2010 | 20920526 |
| a new assay to determine oritavancin concentrations. | | 2010 | 20940724 |
| diversity and activity of cultivable aerobic planktonic bacteria of a saline lake located in sovata, romania. | aerobic bacterial strains from the salt water of lake red (sovata, romania) were cultivated. more than half of the 80 strains were g(-) and formed motile straight rods. only a few strains produced acid from d-glucose and reduced nitrate to nitrite. optimum nacl concentration for growth varied between 5 and 15 % in the majority of the strains, so the isolates were regarded moderately halophilic. on the basis of the 16s rrna gene sequence similarity almost half of the strains were identified as me ... | 2010 | 20941581 |
| inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature. | different concentrations of lauricidin (lu, containing 1% lactic acid) and lactic acid alone (la) were evaluated for their effectiveness in reducing naturally occurring microflora of raw chicken breasts. chicken breasts were dipped in 0 (control), 0.5, 1.0, 1.5, and 2.0% solutions of lu (w/v) or la (v/v) for 10, 20, and 30 min and stored at 4°c for 14 d. total plate counts (tpc) and populations of pseudomonas spp. and enterobacteriaceae were determined before and after dipping and after storing ... | 2010 | 20947197 |
| isolation and characterization of novel plant growth promoting micrococcus sp nii-0909 and its interaction with cowpea. | a phosphate-solubilizing bacterial strain nii-0909 isolated from the western ghat forest soil in india was identified as micrococcus sp on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, and 16s rrna gene sequence. the strain exhibited the plant growth-promoting attributes of phosphate solubilization, auxin production, 1-aminocyclopropane-1-carboxylate deaminase activity, and siderophore production. it was able to solubilize (122.4μg ... | 2010 | 20951599 |
| a pilot study of bioaerosol reduction using an air cleaning system during dental procedures. | bioaerosols are defined as airborne particles of liquid or volatile compounds that contain living organisms or have been released from living organisms. the creation of bioaerosols is a recognized consequence of certain types of dental treatment and represents a potential mechanism for the spread of infection. | 2010 | 20953167 |
| bacteriostatic activity of the proregion of human hepcidin. | hepcidin was first identified as an antimicrobial peptide expressed in the liver. it was later demonstrated that hepcidin is in fact the long sought hormone that regulates iron homeostasis in mammals. hepcidin is encoded as an 84 amino acid prepropeptide that is successively cleaved to yield prohepcidin and the mature 25 amino acid hepcidin. both the bioactive 25-aa hepcidin and the 35-aa proregion are secreted by liver hepatocytes. the aim of the present study was to assess the antibacterial ac ... | 2011 | 20955171 |
| microbial contamination of orthodontic buccal tubes from manufacturers. | this study aimed to test the sterility of new unused orthodontic buccal tubes received from manufacturers. four different types of buccal tubes were used straight from the manufactures package without any additional sterilizing step. of these buccal tubes tested, three genera of bacteria, implicated as opportunistic pathogens, namely micrococcus luteus, staphylococcus haemolyticus and acinetobacter calcoaceticus were recovered from these buccal tubes. our data showing microbial contamination on ... | 2010 | 20957099 |
| involvement of apolipophorin iii in antibacterial defense of galleria mellonella larvae. | apolipophorin iii (apolp-iii) is an abundant hemolymph protein involved in lipid transport and immune response in insects. we investigated involvement of apolp-iii in the antibacterial response in galleria mellonella larvae. immune challenge with gram-negative (escherichia coli, klebsiella pneumoniae) and gram-positive (micrococcus luteus) bacteria led to an increase in the level of apolp-iii in g. mellonella hemolymph, 0.5-2h and 8h after treatment, respectively. apolp-iii purified from larval ... | 2010 | 20959145 |
| oxidation of acetyl phosphate and other substrates by micrococcus lysodeikticus. | | 1946 | 21015720 |
| abyssomicin i, a modified polycyclic polyketide from streptomyces sp. chi39. | abyssomicin i (1), a new modified polycyclic polyketide, was isolated from the culture extract of a soil-derived streptomyces sp. the structure of 1 was elucidated by interpretation of nmr and other spectroscopic data. the stereochemistry of the new compound was assigned by noe analysis, chemical derivatization, and application of the modified mosher method. while 1 was inactive against bacteria and yeasts, the oxidized derivative 7 showed weak activities against gram-positive bacteria. compound ... | 2010 | 21028891 |
| heterogeneous precipitation of silver nanoparticles on kaolinite plates. | two different methods to obtain silver nanoparticles supported on kaolin crystals have been performed: the first one followed a thermal reduction and the second one a chemical reduction. in both cases, the silver nanoparticles with two different average particles size (ca.12 and 30 nm) were perfectly isolated and attached to the surface of the kaolin plates. the silver nanoparticles were localized mainly at the edge of the single crystal plates, the hydroxyl groups being the main centres of adso ... | 2010 | 21030755 |
| antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model. | the chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: citrus aurantium, c. limon, lavandula angustifolia, matricaria chamomilla, mentha piperita, m. spicata, ocimum basilicum, origanum vulgare, thymus vulgaris and salvia officinalis have been determined. the antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed ... | 2010 | 21030907 |
| 16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull. | marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ... | 2010 | 21038149 |
| diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. | endophytic bacteria have been found in virtually every plant studied, where they colonize the internal tissues of their host plant and can form a range of different beneficial relationships. the diversity of bacterial endophytes associated with ginseng plants of varying age levels in korea was investigated. fifty-one colonies were isolated from the interior of ginseng stems. although a mixed composition of endophyte communities was recovered from ginseng based on the results of 16s rdna analysis ... | 2010 | 21046332 |
| physicochemical parameters influencing coaggregation between the freshwater bacteria sphingomonas natatoria 2.1 and micrococcus luteus 2.13. | the aim of this study was to explore the physicochemical parameters that influence coaggregation between the freshwater bacteria sphingomonas natatoria 2.1 and micrococcus luteus 2.13. using visual coaggregation assays, the effect of different buffers, solutions of differing ionic strength, ph, temperature, and viscosity on the degree of coaggregation was assessed. coaggregation occurred maximally in distilled water but was inhibited when coaggregates were suspended in a commonly-used oral bacte ... | 2010 | 21058055 |
| mannosazide methyl uronate donors. glycosylating properties and use in the construction of β-mannaca-containing oligosaccharides. | mannosazide methyl uronate donors equipped with a variety of anomeric leaving groups (β- and α-s-phenyl, β- and α-n-phenyltrifluoroacetimidates, hydroxyl, β-sulfoxide, and (r(s))- and (s(s))-α-sulfoxides) were subjected to activating conditions, and the results were monitored by (1)h nmr. while the s-phenyl and imidate donors all gave a conformational mixture of anomeric α-triflates, the hemiacetal and β- and α-sulfoxides produced an oxosulfonium triflate and β- and α-sulfonium bistriflates, res ... | 2010 | 21062001 |
| antibacterial activity of the latex of argemone ochroleuca sweet. | to investigate the antibacterial effect of the crude latex of argemone ochroleuca (a. ochroleuca) as antibacterial potential against a range of human pathogenic bacteria. | 2010 | 21063649 |
| crystal structure of heterodimeric hexaprenyl diphosphate synthase from micrococcus luteus b-p 26 reveals that the small subunit is directly involved in the product chain length regulation. | hexaprenyl diphosphate synthase from micrococcus luteus b-p 26 (ml-hexpps) is a heterooligomeric type trans-prenyltransferase catalyzing consecutive head-to-tail condensations of three molecules of isopentenyl diphosphates (c(5)) on a farnesyl diphosphate (fpp; c(15)) to form an (all-e) hexaprenyl diphosphate (hexpp; c(30)). ml-hexpps is known to function as a heterodimer of two different subunits, small and large subunits called hexa and hexb, respectively. compared with homooligomeric trans-pr ... | 2010 | 21068379 |
| comparative evaluation of multi-purpose solutions in the stabilization of tear lysozyme. | the range and extent of tear proteins removed by various multi-purpose solutions has been investigated, but there is little information in the literature about their ability to prevent denaturation of tear proteins, particularly lysozyme. the purpose of this study was to determine the ability of bausch+lomb biotrue™ multi-purpose solution and other care solutions to affect denaturation of lysozyme using a lysozyme activity assay. the test solutions used were: biotrue multi-purpose solution, baus ... | 2010 | 21071258 |
| biotransformation and biomonitoring of phenylurea herbicide diuron. | a gram-positive, micrococcus sp. strain ps-1 isolated from diuron storage site was studied for its capability of biotransformation of phenylurea herbicide diuron to a secondary metabolite, 1-(3,4-dichlorophenyl)urea (dcpu) for bioconjugation and antibody development applications. the metabolite formed associated with profound changes in bacterial cell morphology demonstrated increase in the degradation kinetics of diuron in presence of small quantity of a surfactant. the synthesized metabolite i ... | 2010 | 21075624 |
| bacterial tolerance to silver nanoparticles (snps): aeromonas punctata isolated from sewage environment. | use of silver nanoparticles (snps) is increasing in a large number of consumer products. thus, the possible build-up of the nanoparticles in the environment is becoming a major concern. aeromonas punctata isolated from sewage showed tolerance to 200 μg/ml snps. the growth kinetics data for a. punctata treated with nanoparticles were similar to those in the absence of nanoparticles. there was a reduction in the amount of exopolysaccharides (eps) in bacterial culture supernatant after nanoparticle ... | 2010 | 21077112 |
| enzymes of spz7 phage: isolation and properties. | bacteriophage enzyme preparations exolysin and endolysin were studied. exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated salmonella enteritidis cells. a new method for purification of these enzymes was developed, and their molecular masses were determined. the main catalytic properties of the studied enzymes (ph optimum and specificity to bacterial substrates) were found to be similar. both enzymes lyse escher ... | 2010 | 21077836 |
| destabilase-lysozyme of medicinal leech. multifunctionality of recombinant protein. | preparation and purification of a recombinant protein are described along with characteristics of its specific (for ε-(γ-glu)-lys and d-dimer substrates) and nonspecific (for l-γ-glu-pna) isopeptidase activities; the absence of peptidase function for α-(α-glu)-lys substrate is noted. it is shown that the protein exhibits muramidase (cell walls of micrococcus lysodeikticus) and specific glycosidase activities. the latter was determined towards the fluorogenic substrate 4-methylumbelliferyl-tetra- ... | 2010 | 21077838 |
| biological and docking studies of topoisomerase iv inhibition by thiosemicarbazides. | 4-benzoyl-1-(4-methyl-imidazol-5-yl)-carbonylthiosemicarbazide (1) was synthesized, and its antibacterial and type iia topoisomerase (dna gyrase and topoisomerase iv) activity evaluated. (1) was found to have high therapeutic potential against opportunistic gram-positive bacteria, and inhibitory activity against topoisomerase iv (ic(50) = 90 μm) but not against dna gyrase. an increase in activity against topoisomerase iv (ic(50) = 14 μm) was observed when the imidazole moiety of (1) was replaced ... | 2010 | 21080016 |
| phytoremediation of metals from fly ash through bacterial augmentation. | different combinations of four bacterial strains isolated from fly ash were used by us to study their impact on phytoextraction of metals from fly ash by brassica juncea grown in fly ash amended with farm yard manure (50:50 w/w). out of 11 bacterial consortia, a combination of two strains i.e. paenibacillus macerans nbrft5 + bacillus pumilus nbrft9 (c7) inoculated in the rhizosphere was found to enhance pb accumulation maximally by 278%, mn by 75%, zn by 163%, cr by 226% and ni by 414% compared ... | 2010 | 21080221 |
| microbiological contamination of cubicle curtains in an out-patient podiatry clinic. | abstract: | 2010 | 21087486 |
| antiseptic activity and phenolic constituents of the aerial parts of vitex negundo var. cannabifolia. | four phenolics, salviaplebeiaside (1), γ-tocopherol (2), chrysosplenol-d (4), and isovitexin (5), along with α-tocoquinone (3) and β-sitosterol (6) were isolated from the aerial parts of vitex negundo var. cannabifolia. the isolation was performed using bio-assay tracking experiments. the structures of compounds 1-5 were established by spectroscopic means. the antibacterial activities of the compounds were assessed against escherichia coli, bacillus subtilis, micrococcus tetragenus and pseudomon ... | 2010 | 21088661 |
| effect of flash-heat treatment on antimicrobial activity of breastmilk. | abstract background and objectives: the world health organization recommends human immunodeficiency virus (hiv)-positive mothers in resource-poor regions heat-treat expressed breastmilk during periods of increased maternal-to-child transmission risk. flash-heat, a "low tech" pasteurization method, inactivates hiv, but effects on milk protein bioactivity are unknown. the objectives were to measure flash-heat's effect on antimicrobial properties of lactoferrin, lysozyme, and whole milk and on the ... | 2010 | 21091243 |
| characterization and antioxidative activities of rare c₅₀ carotenoids-sarcinaxanthin, sarcinaxanthin monoglucoside, and sarcinaxanthin diglucoside-obtained from micrococcus yunnanensis. | while screening for antioxidative carotenoids from marine bacteria, we isolated and identified sarcinaxanthin and its glucosylated compounds (sarcinaxanthin monoglucoside and sarcinaxanthin diglucoside) from a moderately halophilic bacterium-micrococcus yunnanensis strain aoy-1. in the singlet oxygen ((¹o₂) quenching model, the ic₅₀ values of the antioxidative activities of these carotenoids were as follows: sarcinaxanthin , 57 µm; sarcinaxanthin monoglucoside, 54 µm; and sarcinaxanthin diglucos ... | 2010 | 21099143 |
| isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil. | in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ... | 2010 | 21124065 |
| comparison of antibacterial activity in the hemolymph of marine bivalves from galicia (nw spain). | a screening study of in vitro antibacterial activity was conducted in marine bivalves with economical importance and widespread along the coast of galicia (nw spain). hemocyte lysate supernatant (hls) and plasma of mytilus galloprovincialis, ostrea edulis, crassostrea gigas, ruditapes decussatus, ruditapes philippinarum, and cerastoderma edule were incubated with vibrio splendidus and micrococcus sp. hls and plasma for all the species demonstrated antibacterial activity, and c. edule had the hig ... | 2010 | 21134379 |
| characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein. | lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ... | 2010 | 21167286 |
| characterization of six antibacterial response genes from the european corn borer (ostrinia nubilalis) larval gut and their expression in response to bacterial challenge. | six cdnas encoding putative antibacterial response proteins were identified and characterized from the larval gut of the european corn borer (ostrinia nubilalis). these antibacterial response proteins include four peptidoglycan recognition proteins (pgrps), one β-1,3-glucanase-1 (βglu-1), and one lysozyme. tissue-specific expression analysis showed that these genes were highly expressed in the midgut, except for lysozyme. analysis of expression of these genes in different developmental stage sho ... | 2010 | 21167833 |
| exposure level and distribution characteristics of airborne bacteria and fungi in seoul metropolitan subway stations. | the exposure level and distribution characteristics of airborne bacteria and fungi were assessed in the workers' activity areas (station office, bedroom, ticket office and driver's seat) and passengers' activity areas (station precinct, inside the passenger carriage, and platform) of the seoul metropolitan subway. among investigated areas, the levels of airborne bacteria and fungi in the workers' bedroom and station precincts were relatively high. no significant difference was found in the conce ... | 2010 | 21173524 |
| a versatile reagent to synthesize diverse ionic liquids ranging from small molecules and dendrimers to functionalized proteins. | an ionic liquid "reagent" bearing a succinimidyl activated ester is reported that can be used to synthesize a variety of small molecule and macromolecular ionic liquids. in addition, the ionic liquid reagent was used to modify lysozyme, and the protein retained its structure and function after modification. this study describes a facile and reliable route to new ionic liquid compositions. | 2010 | 21180737 |
| bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of lonar, india. | phenol is an industrially important compound which has a wide range of applications. being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. because of its toxicity, bioremediation of phenol is necessary. since some of the phenol-bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. alkaliphilic bacteria were ... | 1998 | 21182701 |
| the g-type lysozyme of scophthalmus maximus has a broad substrate spectrum and is involved in the immune response against bacterial infection. | lysozyme is a muramidase that inflicts damage on bacterial cell wall by catalyzing the cleavage of the beta-1,4-glycosidic bond between n-acetylmuramic acid and n-acetylglucosamine in peptidoglycan. lysozymes are classified into several types, one of which is the goose-type (g-type). in this study, we identified and analyzed a g-type lysozyme (smlysg) from turbot scophthalmus maximus. the deduced amino acid sequence of smlysg contains 193 residues and is most closely related to that of the g-typ ... | 2010 | 21185386 |
| the cell wall teichuronic acid synthetase (tuas) is an enzyme complex located in the cytoplasmic membrane of micrococcus luteus. | the cell wall teichuronic acid (tua) of micrococcus luteus is a long-chain polysaccharide composed of disaccharide repeating units [-4-β-d-mannacap-(1→6)α-d-glcp-1-](n), which is covalently anchored to the peptidoglycan on the inner cell wall and extended to the outer surface of the cell envelope. an enzyme complex responsible for the tua chain biosynthesis was purified and characterized. the 440 kda enzyme complex, named teichuronic acid synthetase (tuas), is an octomer composed of two kinds of ... | 2010 | 21188072 |
| identification of streptomyces sp. kh29, which produces an antibiotic substance processing an inhibitory activity against multidrug-resistant acinetobacter baumannii. | the actinomycete strain kh29 is antagonistic to the multidrug-resistant acinetobacter baumannii. based on the diaminopimelic acid (dap) type, and the morphological and physiological characteristics observed through the use of scanning electron microscopy (sem), kh29 was confirmed as belonging to the genus streptomyces. by way of its noted 16s rdna nucleotide sequences, kh29 was found to have a relationship with streptomyces cinnamonensis. the production of an antibiotic from this strain was foun ... | 2010 | 21193822 |
| focusing and mobilization of bacteria in capillary electrophoresis. | an isotachophoretic method has been developed for mobilizing and focusing bacteria. this allows quantification and detection of bacteria in a narrow zone. very good linearity was obtained for micrococcus lysodeikticus (also called micrococcus luteus, studied as a model of gram+ bacteria) in the range of 0.4 × 10(8) cells/ml to 2.9 × 10(8) cells/ml, with correlation coefficients for peak height and peak area as a function of cell concentration of 0.999 and 0.998, respectively. this method is usab ... | 2011 | 21204573 |
| gram-negative and gram-positive antibacterial properties of the whole plant extract of willow herb (epilobium angustifolium). | the emergence of new pathogens and the increase in the number of multidrug-resistant strains in well-established pathogens during the past decade represent a growing public health concern globally. with the current lack of research and development of new antibiotics by large pharmaceutical companies due to poor financial returns, new alternatives need to be explored including natural herbal or plant-based extracts with reported antibacterial properties. willow herb (epilobium angustifolium) prep ... | 2011 | 21208973 |
| synthesis and evaluation of a class of new coumarin triazole derivatives as potential antimicrobial agents. | a series of new coumarin-based 1,2,4-triazole derivatives were designed, synthesized and evaluated for their antimicrobial activities in vitro against four gram-positive bacteria (staphylococcus aureus, mrsa, bacillus subtilis and micrococcus luteus), four gram-negative bacteria (escherichia coli, proteus vulgaris, salmonella typhi and shigella dysenteriae) as well as three fungi (candida albicans, saccharomyces cerevisiae and aspergillus fumigatus) by two-fold serial dilution technique. the bio ... | 2010 | 21215620 |
| two novel antimicrobial peptides, arasin-likesp and grpsp, from the mud crab scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria. | antimicrobial peptides (amps) are some of the important host molecules required to resist pathogen infection. two novel amps (arasin-likesp and grpsp) were identified from the hemocytes of the mud crab, scylla paramamosain, by analysis of a hemocyte expressed sequence tag library. the deduced open reading frames of the arasin-likesp and grpsp cdnas are 198 and 168 bp, and encode for predicted peptides of 65 and 55 amino acid residues, respectively. the calculated molecular mass of the mature pep ... | 2011 | 21220028 |
| enhanced microbial degradation of humin-bound phenanthrene in a two-liquid-phase system. | humin, the main component of soil organic matter, greatly influences the nonlinear sorption and desorption hysteresis of polycyclic aromatic hydrocarbons (pahs) in soil. however, little is known about the bioavailability of pahs bound to humin. in the present study, a phenanthrene (phe)-degrading bacterial strain--phe9--was isolated and identified as the genus micrococcus. it was used to investigate the degradation of humin-bound phe and phe not bound to humin (non-humin phe) in liquid mineral m ... | 2010 | 21232850 |
| a galectin with quadruple-domain from bay scallop argopecten irradians is involved in innate immune response. | galectins are a family of β-galactoside-binding lectins that specifically bind to β-galactoside residues and play crucial roles in innate immune responses of invertebrates and vertebrates. the cdna of bay scallop argopecten irradians galectin (designated as aigal2) was cloned by rapid amplification of cdna ends (race) method based on the expressed sequence tag (est). the full-length cdna of aigal2 was of 2137 bp. the open reading frame encoded a polypeptide of 555 amino acids containing four car ... | 2011 | 21237194 |
| micrococcus lactis sp. nov., isolated from a dairy industry waste treatment. | a gram-positive, yellow pigmented, actinobacterial strain dw152t was isolated from a dairy industry effluent treatment plant. the 16s rrna gene sequence analysis indicated that strain dw152t exhibited low similarity with many validly published species belonging to genera micrococcus and arthrobacter. however, phenotypic properties including chemotaxonomic markers affiliated strain dw152t to the genus micrococcus. strain dw152t had ai-c15:0 and i-c15:0 as major cellular fatty acids, and mk-8(h2) ... | 2011 | 21239567 |
| expression of apostichopus japonicus lysozyme in the methylotrophic yeast pichia pastoris. | apostichopus japonicus (sea cucumber) is one of the economically important farmed echinoderm species in northern china. as a crucial enzyme in innate immunity, lysozyme plays a key role in the overall defense against pathogens in a. japonicus. in the present study, a lysozyme gene from a. japonicus was cloned by pcr and expressed in pichia pastoris using the expression vector ppic9k. the expressed lysozyme had a molecular mass of ∼14 kd, as shown by sds-page and western-blotting. the expression ... | 2011 | 21241808 |
| polymer/bacteria composite nanofiber non-wovens by electrospinning of living bacteria protected by hydrogel microparticles. | physically crosslinked pva-hydrogel microparticles are utilized for encapsulation of e. coli and m. luteus. the bacteria survive dry storage or treatment with bacteria-hostile organic solvents significantly better than unprotected bacteria as proven by culture-test experiments. the bacteria-protecting pva microparticles are available for standard polymer-solution-processing techniques, as exemplarily shown by co-electrospinning of living bacteria encapsulated in dry pva-hydrogel microparticles t ... | 2011 | 21243634 |
| long-term surveillance of air quality in medical center operating rooms. | maintenance of adequate indoor air quality (iaq) in operating rooms (ors) is critical to the prevention of nosocomial infection in hospitalized patients. this study evaluated the characteristics of iaq in various ors in a medical center. | 2011 | 21256628 |
| minimal antimicrobial peptidic sequence from hemoglobin alpha-chain: kyr. | hemoglobin is an animal protein described as a source of biologically active peptides. peptic digestion of bovine hemoglobin alpha-chain allowed obtaining peptide fractions with antimicrobial activity. these peptides were purified by reverse-phase high-performance liquid chromatography (hplc) and characterized by mass spectrometry. the minimal inhibitory concentration and mode of action of these peptides were studied against five bacterial strains including escherichia coli and salmonella enteri ... | 2011 | 21262306 |
| recognition of microbial molecular patterns and stimulation of prophenoloxidase activation by a ß-1,3-glucanase-related protein in manduca sexta larval plasma. | detection of pathogenic invaders is the essential first step of a successful defense response in multicellular organisms. in this study, we have identified a new member of the ß-1,3-glucanase-related protein superfamily from the tobacco hornworm manduca sexta. this protein, designated microbe binding protein (mbp), is 61% identical in sequence to bombyx mori gram-negative bacteria binding protein, but only 34-36% identical to m. sexta ß-1,3-glucan recognition protein-1 and 2. its mrna levels wer ... | 2011 | 21296155 |
| extracellular polymeric substances enhanced mass transfer of polycyclic aromatic hydrocarbons in the two-liquid-phase system for biodegradation. | the objective was to elucidate the role of extracellular polymeric substances (eps) in biodegradation of polycyclic aromatic hydrocarbons in two-liquid-phase system (tlps). therefore, biodegradation of phenanthrene (phe) was conducted in a typical tlps--silicone oil-water--with phe-degrading bacteria capable of producing eps, sphingobium sp. phe3 and micrococcus sp. phe9. the results showed that the presence of both strains enhanced mass transfer of phe from silicone oil to water, and that biode ... | 2011 | 21327962 |
| heat treatment effects on the antimicrobial activity of macrolide and lincosamide antibiotics in milk. | antibiotic residues in milk can cause serious problems for consumers and the dairy industry. heat treatment of milk may diminish the antimicrobial activity of these antibiotic residues. this study analyzed the effect of milk processing (60 °c for 30 min, 120 °c for 20 min, and 140 °c for 10 s) on the antimicrobial activity of milk samples fortified with three concentrations of three macrolides (erythromycin: 20, 40 and 80 µg/liter; spiramycin: 100, 200, and 400 µg/liter; and tylosin: 500, 1,000, ... | 2011 | 21333154 |
| continuous control of the flow in biochemical pathways through 5' untranslated region sequence modifications in mrna expressed from the broad-host-range promoter pm. | the inducible pm promoter integrated into broad-host-range plasmid rk2 replicons can be fine-tuned continuously between the uninduced and maximally induced levels by varying the inducer concentrations. to lower the uninduced background level while still maintaining the inducibility for applications in, for example, metabolic engineering and synthetic (systems) biology, we report here the use of mutations in the pm dna region corresponding to the 5' untranslated region of mrna (utr). five utr var ... | 2011 | 21335387 |
| scalarane sesterterpenes from the sponge hyatella sp. | five new sesterterpenes (7-11) along with six known compounds (1-6) were isolated from the sponge hyatella sp., collected off the coast of soheuksan-do, korea. spectroscopic analyses revealed these compounds as scalarane sesterterpenes with oxidized furan moieties (7-10) and a corresponding lactam (11). the compounds exhibited moderate cytotoxicity, antibacterial activity, and weak inhibitory activity against isocitrate lyase. | 2011 | 21341710 |
| reducing blood culture contamination in a pediatric emergency department. | blood cultures (bcs) are used to diagnose bacteremia in febrile children. false-positive bcs increase costs because of further testing, longer hospital stays, and unnecessary antibiotic therapy. data from a study at our hospital showed the emergency department consistently exceeded established guidelines of 2% to 4%. a phlebotomy policy change was made whereby bc had to be obtained by a second venipuncture and no longer obtained during insertion of intravenous catheters. | 2011 | 21346677 |
| potent antifouling resorcylic acid lactones from the gorgonian-derived fungus cochliobolus lunatus. | three new 14-membered resorcylic acid lactones, two with a rare natural acetonide group and one with a 5-chloro-substituted lactone, named cochliomycins a-c (1-3), together with four known analogues, zeaenol (4), ll-z1640-1 (5), ll-z1640-2 (6), and paecilomycin f (7), were isolated from the culture broth of cochliobolus lunatus, a fungus obtained from the gorgonian dichotella gemmacea collected in the south china sea. their structures and the relative configurations of 1-3 were elucidated using ... | 2011 | 21348465 |
| antibacterial efficacy of raw and processed honey. | in vitro antibacterial activity of methanol, ethanol, and ethyl acetate extracts of raw and processed honey was tested against gram-positive bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, enterococcus faecalis, and micrococcus luteus) and gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, and salmonella typhi). both types of honey showed antibacterial activity against tested organisms with the zone of inhibition (zoi) ranging from 6.94 to 37.94 mm, while e. c ... | 2010 | 21350671 |
| chemical composition and antimicrobial activity of the essential oil of the leaves of feronia elephantum (rutaceae) from north west karnataka. | the essential oil of the leaves of feronia elephantum corr. was analyzed by gas chromatography and gas chromatography/mass spectrometry. the main constituents were beta-pinene (28.4%), z-anethole (22.1%), methyl chavicol (12.0%) and e-anethole (8.1%), among thirty-three identified compounds, which represented 92.6% of the total oil. the antimicrobial activity was tested against five gram-positive and eight gram-negative bacteria, and four fungi. the oil was active against micrococcus luteus (gra ... | 2011 | 21366066 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| antibacterial action of acetic acid soluble material isolated from mucor rouxii and its application onto textile. | acetic acid soluble material (acsm) is a chitosan-rich fraction isolated from the fungal cell wall materials. the final step in the traditional production of fungal chitosan is the separation of chitosan from the cell wall acsm via raising the ph to 9-10 followed by centrifugation. this step results in further undesirable economic and environmental effects. the goal of this paper is to avoid that by investigating the antimicrobial effect of the whole acsm from mucor rouxii dsm-1191 cell wall and ... | 2011 | 21382401 |
| maklamicin, an antibacterial polyketide from an endophytic micromonospora sp. | a new spirotetronate-class polyketide, maklamicin (1), was isolated from the culture extract of an endophytic actinomycete of the genus micromonospora. the structure and relative configuration of 1 were elucidated by interpretation of nmr and other spectroscopic data, and the absolute configuration was determined using the modified mosher method. maklamicin (1) showed strong to modest antimicrobial activity against gram-positive bacteria. | 2011 | 21388191 |
| relative roles of the cellular and humoral responses in the drosophila host defense against three gram-positive bacterial infections. | two nf-kappab signaling pathways, toll and immune deficiency (imd), are required for survival to bacterial infections in drosophila. in response to septic injury, these pathways mediate rapid transcriptional activation of distinct sets of effector molecules, including antimicrobial peptides, which are important components of a humoral defense response. however, it is less clear to what extent macrophage-like hemocytes contribute to host defense. | 2011 | 21390224 |
| antioxidant and antibacterial characteristics of phenolic extracts of locally produced honey in saudi arabia. | the antibacterial and antioxidant properties of 30 selected honey samples produced in saudi arabia have been studied. the inhibitory action of the total phenolic content of the honey samples has been tested against staphylococcus aureus, micrococcus luteus and escherichia coli. the mic values of the ten selected honey samples against s. aureus, m. luteus and e. coli were in the range 0.5-á-¦-á0.2-á--á3.6-á-¦-á0.3; 0.45-á-¦-á0.05-á--á5.0-á-¦-á0.6 and 0.6-á-¦-á0.2-á--á4.4-á-¦-á0.4-ámg-áml(--á1). t ... | 2011 | 21391794 |
| influence of carbon dioxide on the activity of chicken egg white lysozyme. | rapid cooling of shell eggs by using liquid co(2) has shown increased bactericidal effects along with saturation of the egg albumen with co(2). lysozyme is a bactericidal enzyme present in chicken eggs, and it lyses gram-positive bacteria. newly laid chicken eggs have an initial ph of 7.6 to 8.5 and are saturated with co(2). during storage, the ph gradually increases to 9.7, accompanied by a loss of co(2). it is hypothesized that the lysozyme activity is influenced by either co(2) concentration ... | 2011 | 21406377 |
| effect of calcium and magnesium on the antimicrobial action of enterocin lr/6 produced by enterococcus faecium lr/6. | enterococci are well-known producers of antimicrobial peptides (enterocins) that possess potential as biopreservatives in food. in this study, divalent cations and release of intracellular potassium were used to assess the mechanism of interaction and killing of enterocin lr/6 produced by enterococcus faecium lr/6 on three target gram-positive and gram-negative bacteria, namely micrococcus luteus, enterococcus sp. strain lr/3 and escherichia coli k-12. whilst treatment with enterocin lr/6 in all ... | 2011 | 21411293 |
| protein release from biodegradable polyhpma-lysozyme conjugates resulting in bioactivity enhancement. | a novel biodegradable thiazolidine-2-thione functional chain transfer agent was synthesized and employed as a reversible additional fragmentation chain transfer agent to prepare well-defined semitelechelic poly-n-(2-hydroxypropyl) methacrylamides (polyhpmas) with predetermined molecular weights and narrow polydispersities. the protein reactive group, thiazolidine-2-thione, was located at the polymer chain ends fixed by biodegradable disulfide bonds. the functional polyhpma chains were subsequent ... | 2011 | 21416614 |
| synthesis and in vitro microbiological evaluation of novel diethyl 6,6'-(1,4-phenylene)bis(4-aryl-2-oxo-cyclohex-3-enecarboxylates). | novel bis cyclohexenone ester derivatives 14-19 were synthesized and characterized by their spectral data. in vitro microbiological evaluations were carried out for all the novel compounds 14-19 against clinically isolated bacterial and fungal strains. compounds 15, 16, 18 against staphylococcus aureus, 14, 15 against ß-haemolytic streptococcus, 15, 19 against micrococcus luteus, 17, 18 against salmonella typhii, 14, 17 against shigella flexneri, 15 against escherichia coli, 16 against pseudomon ... | 2011 | 21417963 |
| in vitro antioxidant, anticholinesterase and antimicrobial activity studies on three agaricus species with fatty acid compositions and iron contents: a comparative study on the three most edible mushrooms. | the fatty acids of agaricus essettei, agaricus bitorquis and agaricus bisporus were investigated by using gc and gc-ms. the dominant fatty acids were found to be linoleic (61.82-67.29%) and palmitic (12.67-14.71%) acids among the 13 fatty acids detected in the oils. total unsaturation for the oils was calculated as 77.50%, 77.44%, and 79.72%, respectively. in vitro antioxidant, anticholinesterase and antimicrobial activities were also studied. the ethyl acetate extract of agaricus bitorquis show ... | 2011 | 21419821 |
| antimicrobial activity of the essential oil of greek endemic stachys spruneri and its main component, isoabienol. | the essential oils of stachys spruneri boiss. (sample a and sample b) were analyzed by gc and gc-ms. (+)-isoabienol was the dominant component (49.5 and 48.2%, respectively of the total oils) among seventy-two identified constituents. isoabienol was separated, purified by preparative thin-layer chromatography, and further identified by means ofphysicochemical and spectrometric analysis. the microbial growth inhibitory properties of the essential oil and its main metabolite, the labdane diterpene ... | 2011 | 21425694 |
| electron microscopic studies on bactericidal effects of electrolyzed acidic water on bacteria derived from kendo protective equipment. | kendo protective equipment is used without washing for a long time.staphylococcus saprophyticus, micrococcus luteus, andbacillus sphaericus are frequently isolated from the mask ('men' in japanese) of kendo protective equipment during one year. to investigate the bactericidal effects of electrolyzed acidic water on these three bacteria, we observed their cellular structures by electron microscopy after treatment with such water. | 2006 | 21432381 |
| characterization of bioactive recombinant human lysozyme expressed in milk of cloned transgenic cattle. | there is great potential for using transgenic technology to improve the quality of cow milk and to produce biopharmaceuticals within the mammary gland. lysozyme, a bactericidal protein that protects human infants from microbial infections, is highly expressed in human milk but is found in only trace amounts in cow milk. | 2011 | 21436886 |
| topical liposomal dna-repair enzymes in polymorphic light eruption. | polymorphic light eruption (ple) is a very frequent photodermatosis in europe whose pathogenesis may involve resistance to uv-induced immune suppression and simultaneous immune reactions against skin photoneoantigens. we performed a randomized, double-blind, placebo-controlled intra-individual half-body trial to investigate the protective effect of an after-sun (as) lotion containing dna-repair enzymes (photolyase from anacystis nidulans and micrococcus luteus extract with endonuclease activity) ... | 2011 | 21437317 |
| characterisation of immune responses in the pea aphid, acyrthosiphon pisum. | the innate immune system of insects provides effective defence against a range of parasites and pathogens. the pea aphid, acyrthosiphon pisum, is a novel study system for investigating host-parasite interactions due to its complex associations with both well-characterised bacterial symbionts and a diversity of pathogens and parasites, including several important biological control agents. however, little is known about the cellular and humoral immune responses of aphids. here we identify three m ... | 2011 | 21439291 |
| physico-chemical characteristics and free fatty acid composition of dry fermented mutton sausages as affected by the use of various combinations of starter cultures and spices. | the microbiological, physico-chemical and free fatty acid composition of dry fermented mutton sausages were determined during ripening and storage. three sausage mixtures (starter culture [sc], sc and black pepper [sc+bp] and sc, bp and cumin [sc+bp+c]) were compared with a control (co). in general, the lactic acid bacteria populations in the sc+bp increased significantly to 9 log cfu/g and were higher than the co (8 log cfu/g) (p<0.05) from fermentation to ripening. the ph values of the sc, sc+ ... | 2011 | 21458169 |
| deep brain stimulation hardware-related infections: a report of 12 cases and review of the literature. | in this monocentric study, the median delay between deep brain stimulation implantation and infection was 28 days (range, 8-820). infections limited to generator (n = 4) required partial hardware removal, whereas infections involving frontal or retroauricular sites (n = 7) required total removal. surgical samples yielded staphylococcus aureus (n = 6), staphylococcus epidermidis (n = 2), propionibacterium acnes, and micrococcus species. | 2011 | 21460317 |
| resistance of bacterial biofilms formed on stainless steel surface to disinfecting agent. | the natural ability of microorganisms for adhesion and biofilm formation on various surfaces is one of the factors causing the inefficiency of a disinfection agent, despite its proven activity in vitro. the aim of the study was to determine the effectiveness of disinfecting substances on bacterial biofilms formed on stainless steel surface. a universally applied disinfecting agent was used in the tests. bacterial strains: listeria innocua, pseudomonas putida, micrococcus luteus, staphylococcus h ... | 2010 | 21466046 |
| lysozyme coated dna and dna/swnt fibers by solution spinning. | dna fibers were prepared by solution spinning of dna in a lysozyme (lsz) coagulation/gelation bath. strong positive charges carried by lsz protein condensed the dna (strong negative charged) molecules resulting in self-assembly and the formation of fibrillar structures in a gel-like network. dna/lsz fibril formation was found to be dependent on the ratio of dna to lsz. a minimum 0.1 wt.-% of lsz was necessary to condense 0.1 wt.-% of dna into micro-fibrils. macroscopic fiber spinning was possibl ... | 2011 | 21472979 |
| nirk and nirs nitrite reductase genes from non-agricultural forest soil bacteria in thailand. | the genetic heterogeneity of the nitrite reductase gene (nirk and nirs) fragments from denitrifying prokaryotes in a non-agricultural forest soil in thailand was investigated using soil samples from the plant germplasm-royal initiation project area in kanchanaburi province, thailand. soil bacteria were screened for denitrification activity and 13 (from 211) positive isolates were obtained and further evaluated for their ability to reduce nitrate and to accumulate or reduce nitrite. three species ... | 2011 | 21475726 |