| epidemiological study of enterobacteriaceae resistance to colistin in sfax (tunisia). | the authors had for aim to monitor enterobacteriaceae resistance to colistin, during 6 years (2005-2010), and to study the epidemiology of enterobacteriaceae resistant strains isolated in the sfax region (tunisia). | 2012 | 22633474 |
| bactericidal activity of the organo-tellurium compound as101 against enterobacter cloacae. | the antibacterial effect of the organo-tellurium compound as101 on the gram-negative bacterium enterobacter cloacae is shown in this study for the first time. | 2012 | 22628247 |
| animal model methodology: immunocompetent or leucopenic rats, which is the best? results from a model of experimental pneumonia due to derepressed cephalosporinase-producing enterobacter cloacae. | the aim of this study was to compare the bactericidal activity of cefepime plus amikacin against experimental pneumonia induced by a stably derepressed cephalosporinase-producing enterobacter cloacae strain in immunocompetent and leucopenic rats. | 2012 | 22572147 |
| biological characterization of lead-enhanced exopolysaccharide produced by a lead resistant enterobacter cloacae strain p2b. | a lead resistant bacterial strain isolated from effluent of lead battery manufacturing company of goa, india has been identified as enterobacter cloacae strain p2b based on morphological, biochemical characters, fame profile and 16s rdna sequence data. this bacterial strain could resist lead nitrate up to 1.6 mm. significant increase in exopolysaccharide (eps) production was observed as the production increased from 28 to 108 mg/l dry weight when exposed to 1.6 mm lead nitrate in tris buffered m ... | 2012 | 22544353 |
| emergence of shv-12 extended spectrum beta-lactamase among clinical isolates of enterobacter cloacae in tunisia. | a collection of seven multidrug-resistant clinical isolates of enterobacter cloacae with reduced susceptibility to ceftazidime and cefepime recovered from 2009 to 2010 at the university hospital of mahdia, tunisia, was analysed. pcr analysis and sequencing demonstrated that all study isolates harbored shv-12 β-lactamase that was transferred by conjugation. characterization of the regions surrounding the bla(shv-12) showed that this gene was flanked by two is26 elements. pulsed-field gel electroph ... | 2012 | 22543154 |
| characterization of enterobacteriaceae producing oxa-48-like carbapenemases in the uk. | to characterize uk clinical isolates of enterobacteriaceae producing oxa-48-like carbapenemases and to compare their resistance plasmids. | 2012 | 22532467 |
| [cloning and function identification of gene 'adma' and up-stream regulatory sequence related to antagonistic activity of enterobacter cloacae b8]. | to reveal the antagonistic mechanism of b8 strain to xanthomonas oryzae pv. oryzae, transposon tagging method and chromosome walking were deployed to clone antagonistic related fragments around tn5 insertion site in the mutant strain b8b. the function of up-stream regulatory sequence of gene 'adma' involved in the antagonistic activity was further identified by gene knocking out technique. an antagonistic related left fragment of tn5 insertion site, 2 608 bp in length, was obtained by tagging wi ... | 2012 | 22522167 |
| role of inchi2 plasmids harbouring blavim-1, blactx-m-9, aac(6')-ib and qnra genes in the spread of multiresistant enterobacter cloacae and klebsiella pneumoniae strains in different units at hospital vall d'hebron, barcelona, spain. | seven enterobacter cloacae isolates and seven klebsiella pneumoniae isolates harbouring a phenotype compatible with the production of a metallo-β-lactamase were recovered between 2009 and 2011 in three intensive care units of hospital vall d'hebron (barcelona, spain). the presence of bla(vim), bla(imp), bla(ndm), bla(ctx-m), aac(6')-ib, qnra, qnrb and qnrs genes was screened by polymerase chain reaction (pcr) and sequencing. clonal relatedness of the isolates was assessed by pulsed-field gel ele ... | 2012 | 22481058 |
| new role of antibody in bacterial isolation. | to eliminate the interference caused by pseudomonas aeruginosa in the isolation of salmonella, a rabbit polyclonal antibody against p. aeruginosa was prepared by inoculating four new zealand rabbits with the pathogen. the antiserum was purified using saturated ammonium sulfate and added into rappaport-vassiliadis medium with soya (rvs) broth and muller-kauffmann tetrathionate novobiocin broth (mkttn broth) to evaluate whether it could inhibit the growth of p. aeruginosa. observations by scanning ... | 2013 | 22468362 |
| investigating wettability alteration during meor process, a micro/macro scale analysis. | wettability alteration is considered to be one of the important mechanisms that lead to increased oil recovery during microbial enhanced oil recovery (meor) processes. changes in wettability will greatly influence the petrophysical properties of the reservoir rocks and determine the location, flow and distribution of different fluids inside the porous media. understanding the active mechanisms of surface wettability changes by the bacteria would help to optimize the condition for more oil recove ... | 2012 | 22445747 |
| characterization of β-lactamase and porin mutants of enterobacteriaceae selected with ceftaroline + avibactam (nxl104). | ceftaroline + avibactam (nxl104) is a novel inhibitor combination active against enterobacteriaceae with class a and c β-lactamases. we investigated its risk of mutational resistance. | 2012 | 22441578 |
| aromatic hydroxyl group plays a critical role in antibacterial activity of the curcumin analogues. | the aim of this study was to investigate the role of the aromatic substituents of the curcumin scaffold on the antibacterial activity of the resulting curcumin analogues. six curcumin analogues with different aromatic substituents were prepared and their antibacterial activities were evaluated against two gram-positive and four gram-negative bacteria. the structure-activity relationship study demonstrated that antibacterial activity of the curcumin analogues was critically dependent upon the aro ... | 2012 | 22428245 |
| antibacterial and antimycobacterial lignans and flavonoids from larrea tridentata. | three lignans and four flavonoids were isolated and characterized from larrea tridentata and compounds were tested against 16 bacterial species/strains. results showed that: dihydroguaiaretic acid (1) had activity towards methicillin resistant (mr) staphylococcus aureus (minimum inhibitory concentration (mic) 50 µg/ml) and multidrug-resistant (mdr) strains of mycobacterium tuberculosis (mic 12.5-50 µg/ml); 4-epi-larreatricin (2) was active against enterobacter cloacae (mic 12.5 µg/ml), as well a ... | 2012 | 22422605 |
| pathogen identification with laser-induced breakdown spectroscopy: the effect of bacterial and biofluid specimen contamination. | in this paper, the potential use of laser-induced breakdown spectroscopy (libs) for the rapid discrimination and identification of bacterial pathogens in realistic clinical specimens is investigated. specifically, the common problem of sample contamination was studied by creating mixed samples to investigate the effect that the presence of a second contaminant bacterium in the specimen had on the libs-based identification of the primary pathogen. two closely related bacterial specimens, escheric ... | 2012 | 22410932 |
| bloodstream infection with extended-spectrum beta-lactamase-producing enterobacteriaceae at a tertiary care hospital in new zealand: risk factors and outcomes. | to define local risk factors and outcomes for bacteremia with extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-e) at a tertiary hospital in new zealand. | 2012 | 22401750 |
| temporal variation of microbiological and chemical quality of noncarbonated bottled drinking water sold in sri lanka. | use of bottled water in sri lanka has increased over the last decade, while new brands of bottled water are often introduced to the market. however, the manufacturers' adherence to bottled water regulations is questionable, raising concerns regarding the quality of bottled water. the objective of the current study was to investigate the microbiological and chemical quality of bottled water in sri lanka. thirty bottled water brands were sampled and their chemical and microbiological parameters we ... | 2012 | 22384963 |
| occurrence and characteristics of extended-spectrum-β-lactamase- and ampc-producing clinical isolates derived from companion animals and horses. | to investigate the occurrence and characteristics of extended-spectrum β-lactamase (esbl)- and ampc-producing enterobacteriaceae isolates in clinical samples of companion animals and horses and compare the results with esbl/ampc-producing isolates described in humans. | 2012 | 22382469 |
| involvement of curli fimbriae in the biofilm formation of enterobacter cloacae. | in this study, we examined the biofilm forming ability, the mrna expression of curli genes and the morphologies of curli fimbriae and biofilms in clinical isolates of enterobacter cloacae. the csgba operon was found in 11 (78.6%) of the 14 isolates. the ability of e. cloacae isolates to form biofilms was significantly correlated with the mrna expression level of the csga and csgd genes. the curli protein fimbriae appeared as tangled fibers and the curli-proficient strain formed mature biofilms. ... | 2012 | 22367955 |
| comparison of the genetic structures surrounding qnra1 in korean enterobacter cloacae and chinese escherichia coli strains isolated in the early 2000s: evidence for qnra mobilization via inc hi2 type plasmid. | the flanking genetic structure of qnra1 in korean enterobacter loacae was identical to that of the chinese escherichia coli strain, the first qnra1-carrying strain reported in asia. analysis of restriction enzyme sites and southern blot hybridization results showed that qnra1 was transferred between e. cloacae and e. coli via inc hi2 type plasmid. | 2012 | 22367953 |
| kinetics and morphology of polymicrobial biofilm formation on polypropylene mesh. | we examined the ability of three clinical bacterial isolates to form mixed biofilms on surgical polypropylene mesh (ppm) in vitro. the three strains--staphylococcus aureus, enterococcus faecalis, and enterobacter cloacae--were isolated from a patient with an infected ppm. staphylococcus aureus and e. faecalis (alone and in combination) were inoculated into culture containing squares of ppm and allowed to attach and propagate into mature biofilms. enterococcus faecalis initially attached to the m ... | 2012 | 22364207 |
| an unusual etiology of infective endocarditis: enterobacter cloacae. | gram-negative microorganisms are rarely implicated in causing infective endocarditis (ie). although the traditionally identified risk factor for gram-negative endocarditis has been intravenous drug abuse, recent studies have revealed that healthcare contact and the presence of prosthetic cardiac devices are primary risk factors for ie secondary to non-hacek gram-negative bacteria. we present a case of enterobacter endocarditis in a patient with no prior history of valvular heart disease, implant ... | 2012 | 22327517 |
| immune-complex-like disease in the course of enterobacter cloacae sepsis due to cholelithic cholecystitis preceded by influenza vaccination. | this case report describes a 52-y-old male patient who, one week after influenza vaccination, presented with abdominal symptoms: pain in the right epigastrium followed by liver dysfunction and pyrexia, as well renal failure, muscles pain and consciousness disorders. immune-complex-like disease and enterobacter cloacae sepsis due to cholelithic cholecystitis were diagnosed. in this case, a correlation between vaccination and immune complex-like-disease was suspected based on the onset of symptoms ... | 2012 | 22327502 |
| photocatalytic disinfection of bacterial pollutants using suspended and immobilized tio2 powders. | the photocatalytic disinfection of enterobacter cloacae and enterobacter coli using microwave (mw), convection hydrothermal (ht) and degussa p25 titania was investigated in suspension and immobilized reactors. in suspension reactors, mw-treated tio(2) was the most efficient catalyst (per unit weight of catalyst) for the disinfection of e. cloacae. however, ht-treated tio(2) was approximately 10 times more efficient than mw or p25 titania for the disinfection of e. coli suspensions in surface wat ... | 2013 | 22296505 |
| how to solve the problem of spontaneous bacterial clearance when testing new antibiotic treatment: results on experimental pneumonia due to a derepressed cephalosporinase-producing enterobacter cloacae. | because the magnitude of spontaneous bacterial clearance can be similar or even higher than treatment effect, depending upon experimental model and bacterial strain used, this work investigated the value of rendering rats immunosuppressed to facilitate bacterial implantation and reduce spontaneous bacterial clearance. in a first step, rats received a single intravenous cyclophosphamide dose 4 days before infection. three different doses were tested: 10, 20, and 40 mg/kg. after modeling with nonm ... | 2013 | 22283475 |
| analysis of scalp wound infections among craniocerebral trauma patients following the 2008 wenchuan earthquake. | to explore the causes of scalp wound infection, pathogen distribution, characteristics of antimicrobial susceptibility and therapeutic measures following craniocerebral trauma caused by the 2008 wenchuan earthquake. material and | 2012 | 22274967 |
| multi-centre evaluation of a phenotypic extended spectrum β-lactamase detection guideline in the routine setting. | this study aimed to evaluate the routine setting performance of a guideline for phenotypic detection of extended spectrum β-lactamases (esbls) in enterobacteriaceae, recommending esbl confirmation with etest or combination disc for isolates with a positive esbl screen test (i.e. cefotaxime and/or ceftazidime mic >1 mg/l or an automated system esbl warning). twenty laboratories submitted 443 enterobacteriaceae with a positive esbl screen test and their confirmation test result (74%escherichia col ... | 2013 | 22268620 |
| prediction of the mode of interaction between monoterpenes and the nitroreductase from enterobacter cloacae by docking simulation. | monoterpenes from the essential oils of several plants have been shown to enhance the bactericidal activities of nitrofurantoin and furazolidone against the bacteria of enterobacteriaceae family. in this study, computer-aided molecular modeling and docking techniques have been employed to simulate the theoretical mode of interaction between monoterpenes and enterobacter cloacae nitroreductase. enhancement of nitro drug potency in the presence of monoterpenes may be the result of modulation of ni ... | 2012 | 22240115 |
| ndm-1-producing enterobacter cloacae and klebsiella pneumoniae from diabetic foot ulcers in india. | | 2012 | 22034164 |
| evaluation of handwashing behaviors and analysis of hand flora of intensive care unit nurses. | the aim of this study was to evaluate the handwashing behaviors of intensive care nurses based on personal statements, and to identify the microorganisms represented in the hand flora preshift and postshift. | 2011 | 25030259 |
| effects of unsaturated uronic acid residues at non-reducing end on bond cleavage frequency of poly(1,4-α-l-guluronide) lyase from enterobacter cloacae m-1. | the mode of action of poly(1,4-α-l-guluronide) lyase from enterobacter cloacae m-1 on unsaturated oligoguluronic acids was studied using fluorophore-assisted carbohydrate electrophoresis. the polyguluronate lyase degraded unsaturated penta-, hexa-, and heptaguluronic acids, but not unsaturated oligoguluronic acids with dps less than 4. on comparison with the aspect of enzymatic degradation of unsaturated oligoguluronic acid and saturated oligoguluronic acid having the same dp, the former was deg ... | 1998 | 27393363 |
| microbiological changes in mawè during natural fermentation. | lactic acid bacteria increased from 3.2 × 10(6) and 1.6 × 10(7) c.f.u./g (wet wt) to 2 × 10(9) and 1.6 × 10(9) c.f.u./g after 12 to 24 h of fermentation of home-produced mawè (a dough produced from dehulled maize) and commercial mawè, respectively. in commercial mawè, the yeast count increased from 1.3 × 10(5) to 2.5 × 10(7) c.f.u./g after 48 h of fermentation before decreasing, whereas in the home-produced mawè it increased from 2.5 × 10(4) to 3.2 × 10(7) c.f.u./g after 72 h of fermentation; th ... | 1994 | 24421087 |
| isolation and characterization of restriction endonuclease eclhki from an enterobacter cloacae strain. | an enterobacter cloacae strain, producing restriction enzyme eclhki, was isolated from a decaying potato. the enzyme is an isoschizomer of eam1105i, which recognizes and cleaves gacnnn!nngtc. eclhki was produced at high activity (4×10(4) u/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through deae-sephacel and heparin columns. activity was optimal at 37°c in a medium salt buffer. | 1994 | 24420881 |
| 3-sulfonyl-1-carba-1-dethiacephems. | the stability of the 1-carba-1-dethiacephalosporin framework has allowed the synthesis of a range of 3-sulfonyl-1-carba-1-dethiacephems unavailable for a variety of reasons in the cephem arena. the known p-nitrobenzyl 7 beta-(phenoxyacetamido)-3-[[(trifluoromethyl)sulfonyl]oxy]-1-carba -1- dethia-3-cephem-4-carboxylate served as a precursor to this series of compounds. displacement of the enol triflate with various sulfinates in acetonitrile or dmf and deprotection of the intermediates led to 7 ... | 1989 | 2810332 |
| bactericidal activity of tigemonam, alone and in combination with gentamicin. | the in vitro bactericidal activity of tigemonam, alone and in combination with gentamicin, was evaluated against various strains of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, salmonella enteritidis, enterobacter cloacae, proteus mirabilis, and proteus vulgaris. minimum inhibitory and bactericidal concentrations were determined on three consecutive days with an inoculum of 10(5) colony-forming units (cfu)/ml or a culture in the logarithmic growth phase. the results found tigemon ... | 1989 | 27416150 |
| antibacterial activity in vitro of tigemonam, a new oral monobactam. | using a broth microdilution method, the antibacterial activity of tigemonam, a novel oral monobactam, was evaluated against 217 gram-negative aerobes freshly isolated from clinical specimems. reference antibiotics were amoxicillin, amoxicillin-sulbactam, cephalexin, cefaclor, norfloxacin, and co-trimoxazole. at a concentration of ≦ 8 mg/l, tigemonam inhibited 88% of the members of the enterobacteriaceae family. its activity was poor against the nonfermenter pathogens. in comparison with the othe ... | 1989 | 27416149 |
| in vitro activity of tigemonam against multiresistant nosocomial enterobacteriaceae. | tigemonam, an oral monobactam that exhibits beta-lactamase stability similar to that of aztreonam, was tested in vitro against 240 species of enterobacteriaceae (50 escherichia coli, 48 klebsiella pneumoniae, 52 enterobacter cloacae, 32 proteus mirabilis, 22 proteus indole-positive [providencia sp.], 24 serrada sp., and 12 citrobacter sp. all strains were resistant to ampicillin and first-generation cephalosporins. in addition, 77.4% were resistant to amoxicillin plus clavulanic acid, 46.8% to c ... | 1989 | 27416147 |
| effect of ph, temperature, and growth conditions on the adhesion of a gliding bacterium and three nongliding bacteria to polystyrene. | the effect of growth rate, growth phase, ph, and temperature on the permanent adhesion of a glidingflexibacter sp. and three nongliding bacteria,pseudomonas fluorescens, enterobacter cloacae, andchromobacterium sp., to polystyrene substrata was investigated. the permanent adhesion of the flexibacter appeared to be related to growth, as levels of adhesion increased with increased growth rate in continuous culture and declined rapidly with death phase in batch culture. with the three nongliding ba ... | 1988 | 24201571 |
| bacteria associated with four species of dacus (diptera: tephritidae) and their role in the nutrition of the larvae. | the bacteria associated with dacus tryoni (froggatt), dacus jarvisi (tryon), dacus neohumeralis (hardy) and dacus cacuminatus (hering) were examined. bacteria were isolated from the surface of freshly-laid eggs, from within surface sterilised pupae, from heads and abdomens of wild and laboratory-maintained flies, and from decomposed fruits in which the wild larvae were feeding. a more diverse flora was associated with d. tryoni and d. jarvisi (15 and 14 species, respectively) than with d. neohum ... | 1985 | 28311582 |
| surface attachment of a sediment isolate ofenterobacter cloacae. | enterobacter cloacae was recovered from surface sediments of a flood control channel in an area where freshwater runoff mixed with coastal seawater. cells of this bacterium elaborated an extensive capsule when cultured under laboratory conditions designed to promote extracellular polysaccharide production. colonization of glass surfaces by cells was similar under aerobic and anaerobic conditions. temperature exerted little effect on maximum adherent cell density in the range of 15-25°c. the avai ... | 1983 | 24221819 |
| successful elimination of extended-spectrum beta-lactamase (esbl)-producing nosocomial bacteria at a neonatal intensive care unit. | extended-spectrum beta-lactamase (esbl)-producing gram-negative bacteria are highly dangerous to neonates. at our neonatal intensive care unit (nicu), the presence of these bacteria became so threatening in 2011 that immediate intervention was required. | 2016 | 27878781 |
| comparison of in vitro efficacy of ertapenem, imipenem and meropenem in the infections caused by the enterobacteriaceae strains family. | the members of the bacterial enterobacteriaceae family play an important role in the aetiology of many hospital infections. some of them are able to produce β-lactamase, an enzyme which induces the resistance of those bacteria to penicillins, cephalosporins and, in severe infections, to penicillins with β-lactamase inhibitors. in this situation, the carbapenems become the drugs of choice. the objective of this study was to analyse the in vitro efficacy of three carbapenems: ertapenem, imipenem a ... | 2014 | 23877897 |
| infections in patients undergoing craniotomy: risk factors associated with post-craniotomy meningitis. | object the authors performed a prospective study to define the prevalence and microbiological characteristics of infections in patients undergoing craniotomy and to clarify the risk factors for post-craniotomy meningitis. methods patients older than 18 years who underwent nonstereotactic craniotomies between january 2006 and december 2008 were included. demographic, clinical, laboratory, and microbiological data were systemically recorded. patient characteristics, craniotomy type, and pre- and p ... | 2015 | 25343179 |
| the oral bacterial microbiome of occlusal surfaces in children and its association with diet and caries. | dental caries is the most prevalent disease in humans globally. efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. this study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. anamneses were recorded from thirteen 12-year-old children. biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16s rrna amplicon sequenci ... | 2017 | 28678838 |
| synthesis of β-galactooligosaccharide using bifidobacterial β-galactosidase purified from recombinant escherichia coli. | galactooligosaccharides (gos) are known to be selectively utilized by bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. in this study, β-gos are synthesized using bifidobacterial β-galactosidase(g1) purified from recombinant e. coli with a high gos yield and with high productivity and enhanced bifidogenic activity. the purified recombinant g1 showed maximum production of β-gos at ph 8.5 and 45°c. a matrix-assisted laser desorption ionization time ... | 2017 | 28535609 |
| evaluation of the rapid polymyxin np test for polymyxin b resistance detection using enterobacter cloacae and enterobacter aerogenes isolates. | background: polymyxin resistance is an increasing problem worldwide. currently, determining susceptibility to polymyxins is problematic and lengthy. polymyxins diffuse poorly into agar, potentially giving inaccurate disk diffusion and etest results. a rapid screening test (2 h) for detection of polymyxin-resistance in enterobacteriaceae, developed by nordmann and poirel (rapid polymyxin np test, 2016), detects glucose metabolization in the presence of polymyxins e and b via ph-induced color chan ... | 2017 | 28747375 |
| multidrug resistant bacteria are sensitive to euphorbia prostrata and six others cameroonian medicinal plants extracts. | multidrug resistant (mdr) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them. | 2017 | 28743314 |
| application of maldi-tof ms fingerprinting as a quick tool for identification and clustering of foodborne pathogens isolated from food products. | foodborne pathogens can be associated with a wide variety of food products and it is very important to identify them to supply safe food and prevent foodborne infections. since traditional techniques are time-consuming and laborious, this study was designed for rapid identification and clustering of foodborne pathogens isolated from various restaurants in al-qassim region, kingdom of saudi arabia (ksa) using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ... | 2017 | 28825446 |
| antimicrobial activity of octenidine against multidrug-resistant gram-negative pathogens. | multidrug-resistant (mr) gram-negative (gn) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. well-tolerated antiseptics, such as octenidine dihydrochloride (oct), may be a very useful tool in infection control to reduce the dissemination of mrgn. this study aimed to investigate the bactericidal activity of oct against international epidemic clones of mrgn. a set of five different species ... | 2017 | 28825186 |
| [analysis of drug resistance of acinetobacter baumannii in wound of children with traffic injury and its relationship with antibiotic use]. | objective: to know the drug resistance of acinetobacter baumannii (ab) in wound of children with traffic injury and its relationship with antibiotic use. methods: wound exudate of 226 children with traffic injury admitted to our unit from january 2010 to december 2015 were collected. api bacteria identification panels and fully automatic microbiological identification system were used to identify pathogens. kirby-bauer paper disk diffusion method was used to detect the drug resistance of pathoge ... | 2017 | 28763905 |
| investigation and analysis of the characteristics and drug sensitivity of bacteria in skin ulcer infections. | skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. | 2017 | 28689800 |
| the distribution of carbapenem- and colistin-resistance in gram-negative bacteria from the tamil nadu region in india. | the occurrence of carbapenem- and colistin-resistance among gram-negative bacteria is increasing worldwide. the aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in tamil nadu, india. | 2017 | 28671537 |
| structure, genetics and worldwide spread of new delhi metallo-β-lactamase (ndm): a threat to public health. | the emergence of carbapenemase producing bacteria, especially new delhi metallo-β-lactamase (ndm-1) and its variants, worldwide, has raised amajor public health concern. ndm-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. | 2017 | 28449650 |
| antimicrobial activity and chemical composition of brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide. | brunfelsia genus is traditionally utilized in popular medicine due to its antibacterial and antifungal properties to name but a few. however, studies on the antimicrobial activity of brunfelsia uniflora flower oleoresin have not been found yet. this study aimed to evaluate the chemical composition and antimicrobial activity of b. uniflora flower oleoresin obtained by supercritical carbon dioxide. oleoresin from the plant dried flowers was obtained by carbon dioxide, and the chemical composition ... | 2017 | 28407186 |
| molecular and functional characterization of myxobacteria isolated from soil in india. | this study reports the isolation of myxobacteria from soil collected from plains in north india. based on the morphology and 16s rdna sequence, the isolated myxobacteria were identified as corallococcus sp., pyxidicoccus sp., myxococcus sp., cystobacter sp. and archangium sp. the myxobacteria were functionally characterized to assess their ability to produce antibacterial and anticancer metabolites. the isolates were found to be functionally versatile as they produced extracellular bioactive mol ... | 2017 | 28567623 |
| novel multifunctional plant growth-promoting bacteria in co-compost of palm oil industry waste. | previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in malaysia. little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. we isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: ... | 2017 | 28736147 |
| a prospective randomised trial of isolated pathogens of surgical site infections (ssi). | every surgical wound is colonized by bacteria, but only a small percentage displays symptoms of infection. the distribution of pathogens isolated in surgical site infections has not significantly changed over the last decades. staph. aureus, coag(-) staphylococci, enterococcus spp and e. coli are the main strains appearing. in addition, a continuously rising proportion of surgical site infections caused by resistant bacterial species (mrsa, c. albicans) has been reported. | 2017 | 28761643 |
| what the eyes don't see, the heart does grieve over. | a 71 year old peruvian woman presented with blurry vision and bilateral eye pain and had evidence of bilateral endophthalmitis on eye examination. her past medical history was notable for multiple abdominal surgeries within the preceding months, including an incarcerated inguinal hernia repair which required an ileostomy placement, and cholecystitis requiring cholecystostomy tube placement. over her multiple long hospitalizations, she developed bacteremia and fungemia on several occasions, with ... | 2017 | 28414678 |
| imipenem/cilastatin : a reappraisal of its antibacterial activity, pharmacokinetic properties and therapeutic efficacy. | imipenem is an antibacterial agent of the carbapenem class of β-lactams, with a very broad spectrum of activity that includes most gram-negative and gram-positive pathogens, aerobes and anaerobes, and with marked activity against species producing β-lactamases. it is coadministered with cilastatin, a renal dehydropeptidase inhibitor that prevents renal metabolism of imipenem. as initial monotherapy, imipenem/cilastatin provides effective and well-tolerated treatment of moderate to severe infecti ... | 1992 | 28634918 |
| the antimicrobial spectrum of xeroform(®). | xeroform(®) is a petrolatum-based fine mesh gauze containing 3% bismuth tribromophenate. bismuth, similar to other metals, has antimicrobial properties. xeroform(®) has been used for decades in burn and plastic surgery as a donor site dressing and as a covering for wounds or partial thickness burns. despite this, the antimicrobial spectrum of xeroform(®) remains largely unknown. we examined the in-vitro efficacy of xeroform(®) against common burn pathogens using zone-of-inhibition methodology in ... | 2017 | 28641915 |
| microbiological analysis of acute infections of the nail fold on the basis of bait thread test. | an acute infection of the nail fold, called paronychia, is a common clinical problem. the basis for the implementation of the treatment is the result of microbiological examination. due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. | 2017 | 28507488 |
| metagenomic sequencing of diamondback moth gut microbiome unveils key holobiont adaptations for herbivory. | herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. our previous study of the genome of diamondback moth, plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. here we expand on that work with a complete taxonomic and functi ... | 2017 | 28491055 |
| occurrence of plasmid- and chromosome-carried mcr-1 in waterborne enterobacteriaceae in china. | the aim of this study was to investigate the prevalence of the polymyxin resistance gene mcr-1 in enterobacteriaceae from environmental water sources in hangzhou, china. colistin-resistant bacteria were isolated from environmental water samples using an enrichment broth culture method, were screened for mcr-1, and then were analyzed for the location and transferability of mcr-1 isolates positive for mcr-1 were further examined to determine their susceptibility profiles and were screened for the ... | 2017 | 28559252 |
| high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria. | we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea. | 2017 | 28805537 |
| insights into a novel blakpc-2-encoding incp-6 plasmid reveal carbapenem-resistance circulation in several enterobacteriaceae species from wastewater and a hospital source in spain. | untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. however, its role in the spread of antibiotic resistances in the human population remains poorly investigated. we used whole genome sequencing to analyze 25 kpc-2-producing enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical citrobacter freundii isolates from a tertiary hospital in the same collection ar ... | 2017 | 28702005 |
| extended-spectrum β-lactamases and plasmid-mediated quinolone resistance in enterobacterial clinical isolates from neonates in tunisia. | this study was conducted to investigate extended-spectrum-β-lactamase (esbl) producing enterobacteriaceae isolates from the center of maternity and neonatology of monastir, tunisia. fourty-six strains out of 283 were found to produce esbl: klebsiella pneumoniae (n = 37), escherichia coli (n = 6), enterobacter cloacae (n = 2), and citrobacter freundi (n = 1). genotyping analysis, using eric2 and rapd, showed that strains were clonally unrelated. pcr amplification followed by sequencing revealed t ... | 2017 | 28648624 |
| molecular and microbiological report of a hospital outbreak of ndm-1-carrying enterobacteriaceae in mexico. | to characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant enterobacteriaceae (cre) in a tertiary-care hospital in mexico. | 2017 | 28636666 |
| the epidemiology, management, and outcomes of bacterial meningitis in infants. | the pathogens that cause bacterial meningitis in infants and their antimicrobial susceptibilities may have changed in this era of increasing antimicrobial resistance, use of conjugated vaccines, and maternal antibiotic prophylaxis for group b streptococcus (gbs). the objective was to determine the optimal empirical antibiotics for bacterial meningitis in early infancy. | 2017 | 28600447 |
| isolation and identification of nonoral pathogenic bacteria in the oral cavity of patients with removable dentures. | dentures in the oral cavity may act as a reservoir of microorganisms, which may be related to systemic infections. the aim of this study was to investigate the nonoral pathogenic bacteria in the oral cavity of patients with removable dentures in shiraz, southern iran. | 2017 | 28852636 |
| high mortality rate associated with kpc-producing enterobacter cloacae in a brazilian hospital. | we describe a clonal dissemination of kpc-producing enterobacter cloacae in a brazilian hospital. patients diagnosed with theses isolates showed high mortality rate (41.8%) and were associated with previous use of antibiotics and urinary catheterization. therefore, infection control measures and use of stricter antibiotic policies are required to control the spread of these organisms. | 2017 | 28844375 |
| persistent gram-negative neurosurgical meningitis in a neonate, successfully treated with intraventricular colistin: case report and review of the literature. | we present a case of gram-negative neurosurgical meningitis in a neonate, refractory to optimal intravenous therapy and removal of ventriculo-peritoneal shunt. cerebrospinal fluid was sterilized within 24 hours using intraventricular colistin. this is the first report of intraventricular colistin use in neonatal meningitis caused by enterobacter cloacae. | 2017 | 28841583 |
| transmission and characterization of bla ndm-1 in enterobacter cloacae at a teaching hospital in yunnan, china. | in recent years, new delhi metallo-beta-lactamases 1 (bla ndm-1) has been reported with increasing frequency and become prevalent. the present study was undertaken to investigate the epidemiological dissemination of the bla ndm-1 gene in enterobacter cloacae isolates at a teaching hospital in yunnan, china. | 2017 | 28830556 |
| antimicrobial activity of ceftazidime-avibactam tested against multidrug-resistant enterobacteriaceae and pseudomonas aeruginosa isolates from united states medical centers (2013--2016). | the in vitro activity of ceftazidime-avibactam and many comparator agents was determined against various resistant subsets of organisms selected among 36,380 enterobacteriaceae and 7,868 pseudomonas aeruginosa. isolates were consecutively collected from 94 us hospitals, and all isolates were tested for susceptibility by reference broth microdilution methods in a central monitoring laboratory (jmi laboratories). enterobacteriaceae isolates resistant to carbapenems (cre) and/or ceftazidime-avibact ... | 2017 | 28827415 |
| draft genome sequence of enterobacter cloacae st520 harbouring blakpc-2, blactx-m-15 and blaoxa-17 isolated from coastal waters of the south atlantic ocean. | aquatic environments have contributed to the dissemination of multidrug-resistant (mdr) bacteria, representing a risk for humans and animals. the aim of this study was to report the first draft genome sequence of a mdr enterobacter cloacae strain recovered from seawater in a public beach in brazil. | 2017 | 28827199 |
| a simple phenotypic method for screening of mcr-1-mediated colistin resistance. | to evaluate a novel method, named colistin-mac test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin mic reduction in the presence of dipicolinic acid (dpa). | 2017 | 28827120 |
| complete genome sequence of enterobacter cloacae 704sk10, an oxa-48-encoding wastewater isolate. | here we present the complete genome sequence of enterobacter cloacae 704sk10, a swiss wastewater isolate encoding an oxa-48 carbapenemase. assembly resulted in closed sequences of the 4,876,946-bp chromosome, a 111,184-bp incf plasmid, and an oxa-48-encoding incl plasmid (63,458 bp) nearly identical to the previously described plasmid poxa-48. | 2017 | 28818912 |
| draft genome sequences of two enterobacter cloacae subsp. cloacae strains isolated from australian hematology patients with bacteremia. | enterobacter cloacae is a common member of the gut microbiota in healthy individuals. however, it is also an opportunistic pathogen, capable of causing bacteremia. we report the draft genomes of two enterobacter cloacae subspecies cloacae strains isolated from hematology patients with bacteremia. both isolates carry genes encoding extended-spectrum β-lactamases. | 2017 | 28818892 |
| rapid phenotypic stress-based microfluidic antibiotic susceptibility testing of gram-negative clinical isolates. | bacteremia is a life-threatening condition for which antibiotics must be prescribed within hours of clinical diagnosis. since the current gold standard for bacteremia diagnosis is based on conventional methods developed in the mid-1800s-growth on agar or in broth-identification and susceptibility profiling for both gram-positive and gram-negative bacterial species requires at least 48-72 h. recent advancements in accelerated phenotypic antibiotic susceptibility testing have centered on the micro ... | 2017 | 28808348 |
| the first enterobacter cloacae co-producing ndm and oxa-48 carbapenemases and interhospital spread of oxa-48 and ndm-producing klebsiella pneumoniae in turkey. | the aim of this study was to investigate the occurrence of carbapenemase-producing enterobacteriaceae. | 2017 | 28792697 |
| the occurrence of ctx-m-25-producing enterobacteriaceae in day-old broiler chicks in japan. | day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercial broiler farm between january and july 2016. sixty-six samples of the paper, which were stained with meconium droppings of the chicks, were collected and examined for isolation of cephalosporin-resistant enterobacteriaceae. cefotaxime (ctx)-resistant klebsiella pneumoniae (1 isolate) and enterobacter cloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples. conjugation experiments revealed that th ... | 2017 | 28781343 |
| treatment of gaseous volatile organic compounds using a rotating biological filter. | rotating biological filter (rbf), which provides higher oxygen mass transfer has been developed for treating gaseous volatile organic compounds (vocs) such as btex (benzene, toluene, ethylbenzene and xylene) at higher concentrations. the screening of enriched cultures has been done initially to enhance the performance of rbf for treating xylene, toluene and xylene, and btex at various loading rates. the removal efficiency of btex was maximum (82%), higher than toluene and xylene (79%), and xylen ... | 2017 | 28780260 |
| antimicrobial resistance of 3 types of gram-negative bacteria isolated from hospital surfaces and the hands of health care workers. | there has been an increased focus in recent years on antimicrobial resistance of bacteria isolated from clinical samples. however, resistance of bacteria from hospital environments has been less frequently investigated. | 2017 | 28780198 |
| photoinactivation of multidrug resistant bacteria by monomeric methylene blue conjugated gold nanoparticles. | multidrug resistant (mdr) bacterial infections have become a severe threat to the community health due to a progressive rise in antibiotic resistance. nanoparticle-based photodynamic therapy (pdt) is increasingly been adopted as a potential antimicrobial option, yet the cytotoxicity associated with pdt is quite unspecific. herein, we show concanavalin-a (cona) directed dextran capped gold nanoparticles (gnpdex-cona) enhanced the efficacy and selectivity of methylene blue (mb) induced killing of ... | 2017 | 28778019 |
| recurrent fuo due to intermittent enterobacter cloacae bacteremias from an infected pacemaker lead diagnosed by gallium scan. | fever of unknown origin (fuo) refers to fevers of ≥101° f that persist for ≥3 weeks and remain undiagnosed after a focused inpatient or outpatient workup. fuo may be due to infectious, malignant/neoplastic, rheumatic/inflammatory, or miscellaneous disorders. recurrent fuos are due to the same causes of classical fuos. recurrent fuos may have continuous or intermittent fevers and are particularly difficult to diagnose. with intermittent fever, recurrent fuo diagnostic tests are best obtained duri ... | 2017 | 28768449 |
| draft genome sequence of an aminoglycoside-resistant rmtd-2-producing enterobacter cloacae subsp. cloacae (st)395 in brazil. | enterobacter cloacae has recently emerged as an important agent of nosocomial infections, due to the diffusion of extended spectrum β-lactamases and carbapenemases in this species. in this context, a raise in the use of aminoglycosides for therapy was noticed, being followed by the accelerated development of resistance mechanisms. in this study, we report the draft genome sequence of a multidrug-resistant enterobacter cloacae subsp. cloacae strain (ec2), isolated from active surveillance culture ... | 2017 | 28757348 |
| identification of an operon involved in fluoride resistance in enterobacter cloacae frm. | fluorine is ubiquitous and the most active non-metal element in nature. while many microorganisms have developed fluoride resistance as a result of the widespread and prolonged application of oral hygiene products, the mechanisms used by these organisms to overcome fluoride toxicity are incompletely understood. in this study, a fluoride-resistant strain, enterobacter cloacae frm, was identified which could grow well at a fluoride concentration of 4,000 mg/l. according to comparative genomics, tr ... | 2017 | 28754999 |
| antimicrobial effects of lysophosphatidylcholine on methicillin-resistant staphylococcus aureus. | methicillin-resistant staphylococcus aureus (mrsa) is an important health care-associated and community-associated pathogen and causes a large number of infections worldwide. for the purpose of application to topical treatment of mrsa infection, we examined the antimicrobial effects of lysophosphatidylcholine (lpc) on mrsa strains. we also investigated the combination effect of lpc and gentamicin on mrsa growth. | 2017 | 28748087 |
| in-vivo electrochemical monitoring of h2o2 production induced by root-inoculated endophytic bacteria in agave tequilana leaves. | a dual-function platinum disc microelectrode sensor was used for in-situ monitoring of h2o2 produced in a. tequilana leaves after inoculation of their endophytic bacteria (enterobacter cloacae). voltammetric experiments were carried out from 0.0 to -1.0v, a potential range where h2o2 is electrochemically reduced. a needle was used to create a small cavity in the upper epidermis of a. tequilana leaves, where the fabricated electrochemical sensor was inserted by using a manual three-dimensional mi ... | 2017 | 28746900 |
| novel class 1 integrons and sequence types in vim-2 and vim-11-producing clinical strains of enterobacter cloacae. | all vim-producing enterobacteriaceae (six enterobacter cloacae) submitted to the argentinian reference laboratory in antimicrobial resistance in the period 2008-13 were characterized. the isolates were referred from 6 nosocomial institutions located in 5 different cities across the country. all isolates showed carbapenem disk diffusion inhibition zones ≤22mm and synergism between a carbapenem disk and edta/sma. the six isolates were pcr positive for blavim. imipenem mics were ≤1 to 8μg/ml. typin ... | 2017 | 28739428 |
| imipenem-resistant gram-negative bacterial isolates carried by persons upon medical examination in korea. | carbapenem-resistant gram-negative bacteria (cr-gnb) have emerged and disseminated worldwide, become a great concern worldwide including korea. the prevalence of fecal carriage of imipenem-resistant gram-negative bacteria (ir-gnb) in persons in korea was investigated. stool samples were collected from 300 persons upon medical examination. samples were screened for ir-gnb by using macconkey agar with 2 μl/ml imipenem. species were identified by 16s rrna gene sequence analysis, and antimicrobial s ... | 2017 | 28721477 |
| introduction of sample tubes with sodium azide as a preservative for ethyl glucuronide in urine. | ethyl glucuronide (etg) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. however, the instability of etg in urine against bacterial degradation or the post-collectional synthesis of etg in contaminated samples may cause false interpretation of etg results in urine samples. this study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism ta ... | 2017 | 28712037 |
| transglycosylation by a chitinase from enterobacter cloacae subsp. cloacae generates longer chitin oligosaccharides. | humans have exploited natural resources for a variety of applications. chitin and its derivative chitin oligosaccharides (chos) have potential biomedical and agricultural applications. availability of chos with the desired length has been a major limitation in the optimum use of such natural resources. here, we report a single domain hyper-transglycosylating chitinase, which generates longer chos, from enterobacter cloacae subsp. cloacae 13047 (ecchi1). ecchi1 was optimally active at ph 5.0 and ... | 2017 | 28698589 |
| surgical site infection with extended-spectrum β-lactamase-producing enterobacteriaceae after cardiac surgery: incidence and risk factors. | to determine the incidence, microbiology and risk factors for sternal wound infection (swi) with extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-pe) following cardiac surgery. | 2017 | 28698036 |
| polymicrobial and monomicrobial infections after spinal surgery: a retrospective study to determine which infection is more severe. | a retrospective clinical review. | 2017 | 28670411 |
| whole-genome sequencing overrules a suspected case of carbapenem-resistant enterobacter cloacae transmission. | | 2017 | 28659317 |
| treatment approach for infection of healed fractures after internal fixation. | to review the efficacy of a treatment approach for patients with infection and colonized implants after open reduction and internal fixation of fractures. | 2017 | 28650945 |
| multidrug-resistant enterobacteriaceae in lebanese hospital wastewater: implication in the one health concept. | extended-spectrum beta-lactamases (esbl) and carbapenemase-producing organisms pose severe problems for hospitalized patients. in the absence of efficient sanitation and sewage disposal, the risks for transmission of hospital organisms into the community are high. our objectives were to study the occurrence and mechanisms of resistance of multidrug-resistant gram-negative bacilli in two lebanese hospital sewage treatment plants. wastewater samples were collected, filtered, and cultivated on macc ... | 2017 | 28650688 |
| structure and gene cluster of the o-antigen of enterobacter cloacae c4115. | an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae c4115 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. the following structure of the linear pentasaccharide repeating unit of the o-polysaccharide was established: →2)-α-l-rhap-(1 → 2)-α-l-rhap-(1 → 2)-α-l-rhap-(1 → 2)-α-d-galp-(1 → 3)-α-d-fucpnac-(1→ the o-antigen gene cluster of e. cloacae c4115 was sequenced. the gene functions were tentatively assign ... | 2017 | 28645003 |
| successful conservative management of a permanent pacemaker pocket infection: a less invasive approach. | we present a successful conservative management strategy for a frail elderly patient with a cardiac resynchronisation pacemaker who presented with evidence of an enterobacter cloacae pacemaker pocket infection. a device washout and debridement procedure was performed, with reburial of the device in a new prepectoral pocket and creation of a closed-loop continuous antibiotic infusion into the infected pacemaker pocket. this was followed by a 6-week course of ambulatory intravenous antibiotic ther ... | 2017 | 28637845 |
| fecal carriage of extended-spectrum β-lactamase- and carbapenemase-producing enterobacteriaceae in egyptian patients with community-onset gastrointestinal complaints: a hospital -based cross-sectional study. | the aim of this study was to determine the prevalence of extended-spectrum β-lactamase (esbl) and carbapenemase production among enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to el-ahrar general hospital, zagazig, egypt in the period between january 2013 and may 2013. | 2017 | 28630686 |
| bacterial biofilm-based catheter-associated urinary tract infections: causative pathogens and antibiotic resistance. | we sought to determine the incidence of bacterial biofilm-based catheter-associated urinary tract infections, identify variables affecting biofilm formation, and identify etiologic bacterial pathogens and antibiotic-resistance patterns associated with biofilm-based catheter-associated urinary tract infections (cautis) in our setup. | 2017 | 28629757 |