| probiotic table olives: microbial populations adhering on olive surface in fermentation sets inoculated with the probiotic strain lactobacillus paracasei impc2.1 in an industrial plant. | this study reports the dynamics of microbial populations adhering on the surface of debittered green olives cv. bella di cerignola in fermentation sets inoculated with the probiotic strain lactobacillus paracasei impc2.1 in different brining conditions (4% and 8% (w/v) nacl) at room temperature and 4 degrees c. the probiotic strain successfully colonized the olive surface dominating the natural lab population and decreasing the ph of brines to <or=5.0 after 30 days until the end of fermentation. ... | 2010 | 20226556 |
| the tango of histone marks and chaperones at replication fork. | nucleosomes are disassembled during dna replication. how histone modifications and histone chaperones collaborate to reassemble nucleosomes on replicated dna is explored by jasencakova et al. (2010) and burgess et al. (2010) in this and a recent issue of molecular cell, respectively. | 2010 | 20227364 |
| dnase i hypersensitivity and epsilon-globin transcriptional enhancement are separable in locus control region (lcr) hs1 mutant human beta-globin yac transgenic mice. | expression of the five beta-like globin genes (epsilon, ggamma, agamma, delta, beta) in the human beta-globin locus depends on enhancement by the locus control region, which consists of five dnase i hypersensitive sites (5'hs1 through 5'hs5). we report here a novel enhancer activity in 5'hs1 that appears to be potent in transfected k562 cells. deletion analyses identified a core activating element that bound to gata-1, and a two-nucleotide mutation that disrupted gata-1 binding in vitro abrogate ... | 2010 | 20231293 |
| sequence characterization and promoter identification of porcine apc10 gene. | apc10 protein, a subunit of the anaphase-promoting complex (apc), plays an essential role in the progression of cells from mitosis to g1. in this study, we cloned and sequenced partial cdna, intron 1 and 5'-flanking sequences of porcine apc10. the partial cdna is 595 bp long and has an open reading frame of 558 bp which encodes 185 putative amino acids. real-time pcr analysis revealed that the porcine apc10 mrna expression shows a wide distribution and expression levels varies within a small dif ... | 2010 | 20232159 |
| conquering the complex world of human septins: implications for health and disease. | septins are highly conserved filamentous proteins first characterized in budding yeast and subsequently identified in must eukaryotes. septins can bind and hydrolyze gtp, which is intrinsically related to their formation of septin hexamers and functional protein interactions. the human septin family is composed of 14 loci, sept1-sept14, which encode dozens of different septin proteins. their central gtpase and polybasic domain regions are highly conserved but they diverge in their n-terminus and ... | 2010 | 20236126 |
| topological network alignment uncovers biological function and phylogeny. | sequence comparison and alignment has had an enormous impact on our understanding of evolution, biology and disease. comparison and alignment of biological networks will probably have a similar impact. existing network alignments use information external to the networks, such as sequence, because no good algorithm for purely topological alignment has yet been devised. in this paper, we present a novel algorithm based solely on network topology, that can be used to align any two networks. we appl ... | 2010 | 20236959 |
| comprehensive screening of human genes with inhibitory effects on yeast growth and validation of a yeast cell-based system for screening chemicals. | to evaluate yeast as a high-throughput cell-based system for screening chemicals that may lead to drug development, 10,302 full-length human cdnas (~50% of the total cdnas) were introduced into yeast. approximately 5.6% (583 clones) of the cdnas repressed the growth of yeast. notably, ~25% of the repressive cdnas encoded uncharacterized proteins. small chemicals can be readily surveyed by monitoring their restorative effects on the growth of yeast. the authors focused on protein kinases because ... | 2010 | 20237203 |
| the availability of vitamins from yeasts the availability to human subjects of riboflavin from fresh and dried bakers' yeasts varying in viability. | | 1947 | 20259197 |
| the fission yeast inhibitor of growth (ing) protein png1p functions in response to dna damage. | in budding yeast and human cells, ing (inhibitor of growth) tumor suppressor proteins play important roles in response to dna damage by modulating chromatin structure through collaborating with histone acetyltransferase or histone deacetylase complexes. however, the biological functions of ing family proteins in fission yeast are poorly defined. here, we report that png1p, a fission yeast ing homolog protein, is required for cell growth under normal and dna-damaged conditions. png1p was further ... | 2010 | 20299455 |
| genetic studies of human dna repair proteins using yeast as a model system. | understanding the roles of human dna repair proteins in genetic pathways is a formidable challenge to many researchers. genetic studies in mammalian systems have been limited due to the lack of readily available tools including defined mutant genetic cell lines, regulatory expression systems, and appropriate selectable markers. to circumvent these difficulties, model genetic systems in lower eukaryotes have become an attractive choice for the study of functionally conserved dna repair proteins a ... | 2010 | 20300059 |
| systematic discovery of nonobvious human disease models through orthologous phenotypes. | biologists have long used model organisms to study human diseases, particularly when the model bears a close resemblance to the disease. we present a method that quantitatively and systematically identifies nonobvious equivalences between mutant phenotypes in different species, based on overlapping sets of orthologous genes from human, mouse, yeast, worm, and plant (212,542 gene-phenotype associations). these orthologous phenotypes, or phenologs, predict unique genes associated with diseases. ou ... | 2010 | 20308572 |
| novel interactors and a role for supervillin in early cytokinesis. | supervillin, the largest member of the villin/gelsolin/flightless family, is a peripheral membrane protein that regulates each step of cell motility, including cell spreading. most known interactors bind within its amino (n)-terminus. we show here that the supervillin carboxy (c)-terminus can be modeled as supervillin-specific loops extending from gelsolin-like repeats plus a villin-like headpiece. we have identified 27 new candidate interactors from yeast two-hybrid screens. the interacting seq ... | 2010 | 20309963 |
| ptph1 dephosphorylates and cooperates with p38gamma mapk to increase ras oncogenesis through pdz-mediated interaction. | protein phosphatases are believed to coordinate with kinases to execute biological functions, but examples of such integrated activities, however, are still missing. in this report, we have identified protein tyrosine phosphatase h1 (ptph1) as a specific phosphatase for p38gamma mitogen-activated protein kinase (mapk) and shown their cooperative oncogenic activity through direct binding. p38gamma, a ras effector known to act independent of its phosphorylation, was first shown to require its uniq ... | 2010 | 20332238 |
| the legionella pneumophila f-box protein lpp2082 (ankb) modulates ubiquitination of the host protein parvin b and promotes intracellular replication. | the environmental pathogen legionella pneumophila encodes three proteins containing f-box domains and additional protein-protein interaction domains, reminiscent of eukaryotic scf ubiquitin-protein ligases. here we show that the f-box proteins of l. pneumophila strain paris are dot/icm effectors involved in the accumulation of ubiquitinated proteins associated with the legionella-containing vacuole. single, double and triple mutants of the f-box protein encoding genes were impaired in infection ... | 2010 | 20345489 |
| integrating gene expression and go classification for pca by preclustering. | gene expression data can be analyzed by summarizing groups of individual gene expression profiles based on go annotation information. the mean expression profile per group can then be used to identify interesting go categories in relation to the experimental settings. however, the expression profiles present in go classes are often heterogeneous, i.e., there are several different expression profiles within one class. as a result, important experimental findings can be obscured because the summar ... | 2010 | 20346140 |
| structural and biochemical characterization of yeast monothiol glutaredoxin grx6. | glutaredoxins (grxs) are a ubiquitous family of proteins that reduce disulfide bonds in substrate proteins using electrons from reduced glutathione (gsh). the yeast saccharomyces cerevisiae grx6 is a monothiol grx that is localized in the endoplasmic reticulum and golgi compartments. grx6 consists of three segments, a putative signal peptide (m1-i36), an n-terminal domain (k37-t110), and a c-terminal grx domain (k111-n231, designated grx6c). compared to the classic dithiol glutaredoxin grx1, grx ... | 2010 | 20347849 |
| selection of rna aptamers imported into yeast and human mitochondria. | in the yeast saccharomyces cerevisiae, nuclear dna-encoded is partially imported into mitochondria. we previously found that the synthetic transcripts of yeast trna(lys) and a number of their mutant versions could be specifically internalized by isolated yeast and human mitochondria. the mitochondrial targeting of trna(lys) in yeast was shown to depend on the cytosolic precursor of mitochondrial lysyl-trna synthetase and the glycolytic enzyme enolase. here we applied the approach of in vitro sel ... | 2010 | 20348443 |
| single and multiple ch (calponin homology) domain containing multidomain proteins in arabidopsis and saccharomyces: an inventory. | genes for individual domains such as ch, lim, ankyrin, ph and rhogap, iq motif, ig_flmn, spectrin, and ef hand probably existed in early evolution before there were plants, fungi or animals so that when we examine multidomain proteins in arabidopsis, saccharomyces, dictyostelium or homo sapiens we encounter various combinations of such domains. while all of these four species express fimbrin and eb1, the lists of ch containing multidomain proteins, however, differ in number and in type for each ... | 2011 | 20349140 |
| conditional cell-wall mutants of saccharomyces cerevisiae as delivery vehicles for therapeutic agents in vivo to the gi tract. | strains of saccharomyces cerevisiae capable of lysis upon conditional down-regulation of cell-wall biogenesis genes (srb1 and pkc1) have been reported. here, we show that they lyse and release recombinant protein not only under laboratory conditions, but (more importantly) under conditions found in the human stomach and duodenum. these findings provide proof that, in principle, such conditional lysis strains could be used as an integral part of a system for the oral delivery of therapeutic prote ... | 2010 | 20356564 |
| arabidopsis thaliana alg3 mutant synthesizes immature oligosaccharides in the er and accumulates unique n-glycans. | the core oligosaccharide glc(3)man(9)glcnac(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (er). the first step of this assembly pathway on the er luminal side is mediated by alg3 (asparagine-linked glycosylation 3), which is a highly conserved reaction among eukaryotic cells. complementary genetics compared with saccharomyces cerevisiae alg gene families and bioinformatic approaches have en ... | 2010 | 20356820 |
| fatp1 inhibits 11-cis retinol formation via interaction with the visual cycle retinoid isomerase rpe65 and lecithin:retinol acyltransferase. | the isomerization of all-trans retinol (vitamin a) to 11-cis retinol in the retinal pigment epithelium (rpe) is a key step in the visual process for the regeneration of the visual pigment chromophore, 11-cis retinal. lrat and rpe65 are recognized as the minimal isomerase catalytic components. however, regulators of this rate-limiting step are not fully identified and could account for the phenotypic variability associated with inherited retinal degeneration (rd) caused by mutations in the rpe65 ... | 2010 | 20356843 |
| application of stopped-flow kinetics methods to investigate the mechanism of action of a dna repair protein. | transient kinetic analysis is indispensable for understanding the workings of biological macromolecules, since this approach yields mechanistic information including active site concentrations and intrinsic rate constants that govern macromolecular function. in case of enzymes, for example, transient or pre-steady state measurements identify and characterize individual events in the reaction pathway, whereas steady state measurements only yield overall catalytic efficiency and specificity. indiv ... | 2010 | 20357752 |
| cloning and functional identification of delta5 fatty acid desaturase gene and its 5'-upstream region from marine fungus thraustochytrium sp. fjn-10. | a gene encoding delta5 fatty acid desaturase (fad5) was cloned from marine fungus thraustochytrium sp. fjn-10, a species capable of producing docosahexaenoic acid. the open reading frame of fad5 was 1,320 bp and encoded a protein comprising 439 amino acids. expression of the fad5 in saccharomyces cerevisiae invsc1 revealed that fad5 is able to introduce a double bond at position 5 of the dihomo-γ-linolenic acid (20:3 δ(8,11,14)), resulting in arachidonic acid (20:4 δ(5,8,11,14)) with a conversio ... | 2011 | 20358240 |
| mutational analyses of trimethylguanosine synthase (tgs1) and mud2: proteins implicated in pre-mrna splicing. | yeast and human tgs1 are orthologous rna cap (guanine-n2) methyltransferases that convert m(7)g caps into the 2,2,7-trimethylguanosine (tmg) caps characteristic of spliceosomal snrnas. tmg caps are dispensable for vegetative yeast growth, but are essential in the absence of mud2, the putative yeast homolog of human splicing factor u2af. here we exploited the synthetic lethal interactions of tgs1delta and mud2delta mutations to identify essential structural features of the tgs1 and mud2 proteins. ... | 2010 | 20360394 |
| the cargo receptor p24a facilitates calcium sensing receptor maturation and stabilization in the early secretory pathway. | the calcium sensing receptor (casr) is a family 3/c g protein-coupled receptor with slow and partial targeting to the plasma membrane in both native and heterologous cells. we identified cargo receptor family member p24a in yeast two-hybrid screens with the casr carboxyl terminus. interactions were confirmed by immunoprecipitation of either p24a or casr in transiently transfected hek293 cells. only the immaturely glycosylated form of casr interacts with p24a. dissociation likely occurs in the en ... | 2010 | 20361938 |
| the bidirectional cytomegalovirus immediate/early promoter is regulated by hog1 and the stress transcription factors sko1 and hot1 in yeast. | the work presented here intends to address the question of whether the immediate/early promoter of cytomegalovirus (cmv), which is widely used for expressing transgenes in eukaryotic cells, yields a constitutive expression of the transgenes under stress conditions in saccharomyces cerevisiae cells. this information would also be relevant because in the tetracycline-regulated expression (teto) system, which is one of the first choices for studying gene function from yeast to human cells, the cmv ... | 2010 | 20364387 |
| targeted cytosine deaminase-uracil phosphoribosyl transferase suicide gene therapy induces small cell lung cancer-specific cytotoxicity and tumor growth delay. | small cell lung cancer (sclc) is a highly malignant cancer for which there is no curable treatment. novel therapies are therefore in great demand. in the present study we investigated the therapeutic effect of transcriptionally targeted suicide gene therapy for sclc based on the yeast cytosine deaminase (ycd) gene alone or fused with the yeast uracil phosphoribosyl transferase (yuprt) gene followed by administration of 5-fluorocytosine (5-fc) prodrug. experimental design: the ycd gene or the ycd ... | 2010 | 20371678 |
| cc3/tip30 affects dna damage repair. | the pro-apoptotic protein cc3/tip30 has an unusual cellular function as an inhibitor of nucleocytoplasmic transport. this function is likely to be activated under conditions of stress. a number of studies support the notion that cc3 acts as a tumor and metastasis suppressor in various types of cancer. the yeast homolog of cc3 is likely to be involved in responses to dna damage. here we examined the potential role of cc3 in regulation of cellular responses to genotoxic stress. | 2010 | 20374651 |
| yet1p and yet3p, the yeast homologs of bap29 and bap31, interact with the endoplasmic reticulum translocation apparatus and are required for inositol prototrophy. | the mammalian b-cell receptor-associated proteins of 29 and 31 kda (bap29 and bap31) are conserved integral membrane proteins that have reported roles in endoplasmic reticulum (er) quality control, er export of secretory cargo, and programmed cell death. in this study we investigated the yeast homologs of bap29 and bap31, known as yet1p and yet3p, to gain insight on cellular function. we found that yet1p forms a complex with yet3p (yet complex) and that complex assembly was important for subunit ... | 2010 | 20378542 |
| identification of malassezia pachydermatis from healthy and diseased human skin. | malassezia pachydermatis is the only species in the genus malassezia that is classically considered to be zoophilic. this yeast is only occasionally isolated from human skin, although it has been found to cause septic epidemics, especially in neonates. the aim of our study was to investigate the prevalence of m. pachydermatis on the skin of patients with malassezia-associated diseases and of healthy subjects. one hundred and sixty skin scrapings from patients with pityriasis versicolor (pv), seb ... | 2009 | 20380109 |
| shugoshin-pp2a counteracts casein-kinase-1-dependent cleavage of rec8 by separase. | during meiosis, the cohesin complexes that maintain sister chromatid cohesion are lost in a stepwise manner. at meiosis i the cohesin subunit rec8 is cleaved only along the chromosome arms; until meiosis ii it is protected at centromeres by the action of shugoshin (sgo1)-protein phosphatase 2a (pp2a). although this regulation hypothetically involves phosphorylation that is antagonized by sgo1-pp2a, the kinase and substrate that are responsible are as yet unknown. using a genetic screen for 'anti ... | 2010 | 20383139 |
| genome-wide analysis of helicase gene family from rice and arabidopsis: a comparison with yeast and human. | helicases are motor proteins which can catalyze the unwinding of stable rna or dna duplex utilizing mainly atp as source of energy. in this study we have identified complete sets of helicases from rice and arabidopsis. the helicase gene family in rice and arabidopsis contains 115 and 113 genes respectively. these helicases were validated based on their annotations and supported with organization of conserved helicase signature motifs. we have also identified homologs of 64 rice rna and dna helic ... | 2010 | 20383562 |
| gtpase activity plays a key role in the pathobiology of lrrk2. | mutations in the leucine-rich repeat kinase 2 (lrrk2) gene are associated with late-onset, autosomal-dominant, familial parkinson's disease (pd) and also contribute to sporadic disease. the lrrk2 gene encodes a large protein with multiple domains, including functional roc gtpase and protein kinase domains. mutations in lrrk2 most likely cause disease through a toxic gain-of-function mechanism. the expression of human lrrk2 variants in cultured primary neurons induces toxicity that is dependent o ... | 2010 | 20386743 |
| an essential dna strand-exchange activity is conserved in the divergent n-termini of blm orthologs. | the gene mutated in bloom's syndrome, blm, encodes a member of the recq family of dna helicases that is needed to suppress genome instability and cancer predisposition. blm is highly conserved and all blm orthologs, including budding yeast sgs1, have a large n-terminus that binds top3-rmi1 but has no known catalytic activity. in this study, we describe a sub-domain of the sgs1 n-terminus that shows in vitro single-strand dna (ssdna) binding, ssdna annealing and strand-exchange (se) activities. t ... | 2010 | 20389284 |
| integrated phosphoproteomics analysis of a signaling network governing nutrient response and peroxisome induction. | phosphorylation of proteins is a key posttranslational modification in cellular signaling, regulating many aspects of cellular responses. we used a quantitative, integrated, phosphoproteomics approach to characterize the cellular responses of the yeast saccharomyces cerevisiae to the fatty acid oleic acid, a molecule with broad human health implications and a potent inducer of peroxisomes. a combination of cryolysis and urea solubilization was used to minimize the opportunity for reorientation o ... | 2010 | 20395639 |
| duality of the carbohydrate-recognition system of pseudomonas aeruginosa-ii lectin (pa-iil). | the study of pseudomonas aeruginosa-ii lectin (pa-iil) complexes with man derivatives as a recognition factor has been neglected since its monomer is a very weak ligand. here, the roles of man oligomers and complexes in pa-iil carbohydrate-recognition were studied by both enzyme-linked lectinosorbent and inhibition assays. from the results obtained, it is proposed that high density weak -oh conformation as seen in yeast mannan is also an important pa-iil recognition factor. this finding provides ... | 2010 | 20398656 |
| atr3 encodes a diflavin reductase essential for arabidopsis embryo development. | *the arabidopsis genome possesses two confirmed cytochrome p450 reductase (cpr) genes, atr1 and atr2, together with a third putative homologue, atr3, which annotation is questionable. *phylogenetic analysis classified atr3 as a cpr-like protein sharing homologies with the animal cytosolic dual flavin reductases, nr1 and fre-1, distinct from the microsomal cprs, atr1 and atr2. like nr1 and fre-1, atr3 lacks the n-terminal endoplasmic reticulum (er) anchor domain of cprs and is localized in the cy ... | 2010 | 20406405 |
| mida is a putative methyltransferase that is required for mitochondrial complex i function. | dictyostelium and human mida are homologous proteins that belong to a family of proteins of unknown function called duf185. using yeast two-hybrid screening and pull-down experiments, we showed that both proteins interact with the mitochondrial complex i subunit ndufs2. consistent with this, dictyostelium cells lacking mida showed a specific defect in complex i activity, and knockdown of human mida in hek293t cells resulted in reduced levels of assembled complex i. these results indicate a role ... | 2010 | 20406883 |
| concomitant mutations in the p450 oxidoreductase and androgen receptor genes presenting with 46,xy disordered sex development and androgenization at adrenarche. | undervirilization in males, i.e. 46,xy disordered sex development (46,xy dsd), is commonly caused by either lack of androgen action due to mutant androgen receptor (ar) or deficient androgen synthesis, e.g. due to mutations in 17alpha-hydroxylase (cyp17a1). like all other microsomal cytochrome p450 (cyp) enzymes, cyp17a1 requires electron transfer from p450 oxidoreductase (por). | 2010 | 20410220 |
| biological diversity of saccharomyces yeasts of spontaneously fermenting wines in four wine regions: comparative genotypic and phenotypic analysis. | combination of molecular genetic analysis (karyotyping, pcr-rflp of met2, the its1-its2 region and the nts region) and physiological examination (melibiose and mannitol utilization, sugar-, ethanol- and copper tolerance, killer activity, fermentation vigor and production of metabolites) of yeasts isolated from spontaneously fermenting wines in four wine regions revealed very high diversity in the saccharomyces cerevisiae populations. practically each s. cerevisiae isolate showed a unique pattern ... | 2010 | 20413169 |
| (1,3)-beta-glucans activate both dectin-1 and nlrp3 inflammasome in human macrophages. | beta-glucans are naturally occurring polysaccharides that are the major cell wall components of fungi. recognition of beta-glucans is mediated through a membrane-bound pattern recognition receptor called dectin-1, and gene knock-out studies have shown that dectin-1 plays an important role in antifungal immune response in vivo. in this report, we have studied the effect of large particulate (1,3)-beta-glucans, including curdlan, glucan from baker's yeast, paramylon, and zymosan, on inflammatory r ... | 2010 | 20421639 |
| nsc126188, a piperazine alkyl derivative, induces apoptosis via upregulation of rhob in hela cells. | we describe here a piperazine alkyl derivative, nsc126188, which induced apoptosis of hela cells by upregulating rhob expression. nsc126188 caused multi-septation of fission yeast and hypersensitized a rho3 mutant, which implicates the involvement of functional human homolog rhob. the treatment of cells with nsc126188 induced apoptosis and a dramatic increase in rhob expression. in addition, rhob knockdown using sirna rescued cells from apoptosis, indicating a crucial role of rhob in nsc126188-i ... | 2010 | 20432054 |
| the effects of selenium supplementation on the spontaneously occurring fibroid tumors of oviduct, 8-hydroxy-2'-deoxyguanosine levels, and heat shock protein 70 response in japanese quail. | oxidative stress is known to be an important contributing factor in many chronic diseases. spontaneously occurring benign oviduct leiomyomas are common tumors of the japanese quail (coturnix coturnix japonica), which makes it a good animal model for screening potential agents for testing in the prevention and treatment of human myoma uteri. since dietary intake of selenium has been associated with a reduced risk of a variety of human cancers, we investigated the effects of selenium supplementati ... | 2010 | 20432170 |
| towards universal systems for recombinant gene expression. | recombinant gene expression is among the most important techniques used both in molecular and medical research and in industrial settings. today, two recombinant expression systems are particularly well represented in the literature reporting on recombinant expression of specific genes. according to searches in the pubmed citation database, during the last 15 years 80% of all recombinant genes reported on in the literature were expressed in either the enterobacterium escherichia coli or the meth ... | 2010 | 20433754 |
| from protein interaction profile to functional assignment: the human protein ki-1/57 is associated with pre-mrna splicing events. | the mapping of protein-protein interactions of a determined organism is considered fundamental to assign protein function in the post-genomic era. as part of this effort, screenings for pairwise interactions by yeast two-hybrid system have been used popularly to reveal protein interaction networks in different biological systems. through the identification of protein interaction partners we have successfully obtained interesting functional clues for ki-1/57, a human protein with no previous func ... | 2010 | 20436279 |
| [metacaspases and their role in the life cycle of human protozoan parasites]. | metacaspases are caspase-related cysteine-proteases that are present in organisms devoid of caspases such as plants, yeast, and protozoan parasites. since caspases are important effector molecules in mammalian apoptosis, the possible role of metacaspases in programmed cell death has been evaluated in the organisms where they are expressed. in some species of the human protozoan parasites trypanosoma spp. and leishmania spp., metacaspases have been involved in programmed cell death, although a ro ... | 2009 | 20436999 |
| detection of gene orthology from gene co-expression and protein interaction networks. | ortholog detection methods present a powerful approach for finding genes that participate in similar biological processes across different organisms, extending our understanding of interactions between genes across different pathways, and understanding the evolution of gene families. | 2010 | 20438654 |
| hormone activity of hydroxylated polybrominated diphenyl ethers on human thyroid receptor-beta: in vitro and in silico investigations. | hydroxylated polybrominated diphenyl ethers (ho-pbdes) may disrupt thyroid hormone status because of their structural similarity to thyroid hormone. however, the molecular mechanisms of interactions with thyroid hormone receptors (trs) are not fully understood. | 2010 | 20439171 |
| recombinant yeast screen for new inhibitors of human acetyl-coa carboxylase 2 identifies potential drugs to treat obesity. | acetyl-coa carboxylase (acc) is a key enzyme of fatty acid metabolism with multiple isozymes often expressed in different eukaryotic cellular compartments. acc-made malonyl-coa serves as a precursor for fatty acids; it also regulates fatty acid oxidation and feeding behavior in animals. acc provides an important target for new drugs to treat human diseases. we have developed an inexpensive nonradioactive high-throughput screening system to identify new acc inhibitors. the screen uses yeast gene- ... | 2010 | 20439761 |
| autologous extracellular cytochrome c is an endogenous ligand for leucine-rich alpha2-glycoprotein and beta-type phospholipase a2 inhibitor. | beta-type phospholipase a(2) inhibitory protein (plibeta) from the serum of the venomous snake gloydius brevicaudus neutralizes basic phospholipase a(2) (pla(2)) from its own venom, and it has 33% sequence homology with human leucine-rich alpha(2)-glycoprotein (lrg), which has been recently reported to bind cytochrome c (cyt c) (cummings, c., walder, j., treeful, a., and jemmerson, r. (2006) apoptosis 11, 1121-1129). in the present study, plibeta was found to bind cyt c. the interactions of lrg ... | 2010 | 20442399 |
| characterizing the interaction between the rab6 gtpase and mint3 via flow cytometry based fret analysis. | in extension to previously applied techniques like yeast two-hybrid and gst pull-down assays, we successfully established a facs-based fret analysis to investigate the interaction of the mint3 adaptor protein and the small rab gtpase rab6a in living mammalian cells. a mint3 mutant containing only the ptb domain (mint3delta6) is able to interact with the constitutively active form of rab6a. mint3delta4, a mutant lacking part of the ptb domain was unable to interact with rab6a in gst pull-down ana ... | 2010 | 20447381 |
| delineation of wrn helicase function with exo1 in the replicational stress response. | the wrn gene defective in the premature aging disorder werner syndrome encodes a helicase/exonuclease. we examined the ability of wrn to rescue dna damage sensitivity of a yeast mutant defective in the rad50 subunit of mre11-rad50-xrs2 nuclease complex implicated in homologous recombination repair. genetic studies revealed wrn operates in a yexo1-dependent pathway to rescue rad50 sensitivity to methylmethane sulfonate (mms). wrn helicase, but not exonuclease, is required for mms resistance. wrn ... | 2010 | 20447876 |
| chd8 interacts with chd7, a protein which is mutated in charge syndrome. | charge syndrome is an autosomal dominant disorder caused in about two-third of cases by mutations in the chd7 gene. for other genetic diseases e.g. hereditary spastic paraplegia, it was shown that interacting partners are involved in the underlying cause of the disease. these data encouraged us to search for chd7 binding partners by a yeast two-hybrid library screen and chd8 was identified as an interacting partner. the result was confirmed by a direct yeast two-hybrid analysis, co-immunoprecipi ... | 2010 | 20453063 |
| cloning and expression of functional full-length human tissue plasminogen activator in pichia pastoris. | human tissue plasminogen activator (t-pa) plays a pivotal role in the treatment of acute myocardial infarction, ischemic stroke, and deep vein thrombosis. it has the benefit of generating no adverse effects such as fibrinogen depletion, systemic hemorrhage, and immunologic reactions. human t-pa is a serine-protease enzyme containing 527 amino acid residues in five structural domains. the correct folding of t-pa requires the correct pairing of 17 disulfide bridges in the molecule. a gene encoding ... | 2010 | 20455033 |
| the evolutionary landscape of the chromatin modification machinery reveals lineage specific gains, expansions, and losses. | model organisms such as yeast, fly, and worm have played a defining role in the study of many biological systems. a significant challenge remains in translating this information to humans. of critical importance is the ability to differentiate those components where knowledge of function and interactions may be reliably inferred from those that represent lineage-specific innovations. to address this challenge, we use chromatin modification (cm) as a model system for exploring the evolutionary pr ... | 2010 | 20455264 |
| mds3 regulates morphogenesis in candida albicans through the tor pathway. | the success of candida albicans as a major human fungal pathogen is dependent on its ability to colonize and survive as a commensal on diverse mucosal surfaces. one trait required for survival and virulence in the host is the morphogenetic yeast-to-hypha transition. mds3 was identified as a regulator of ph-dependent morphogenesis that functions in parallel with the classic rim101 ph-sensing pathway. microarray analyses revealed that mds3 delta/delta cells had an expression profile indicative of ... | 2010 | 20457806 |
| substrate specificity of the tim22 mitochondrial import pathway revealed with small molecule inhibitor of protein translocation. | the tim22 protein import pathway mediates the import of membrane proteins into the mitochondrial inner membrane and consists of two intermembrane space chaperone complexes, the tim9-tim10 and tim8-tim13 complexes. to facilitate mechanistic studies, we developed a chemical-genetic approach to identify small molecule agonists that caused lethality to a tim10-1 yeast mutant at the permissive temperature. one molecule, mitoblock-1, attenuated the import of the carrier proteins including the adp/atp ... | 2010 | 20457929 |
| engineering of a human kringle domain into agonistic and antagonistic binding proteins functioning in vitro and in vivo. | here, we report the development of target-specific binding proteins based on the kringle domain (kd) ( approximately 80 residues), a ubiquitous modular structural unit occurring across eukaryotic species. by exploiting the highly conserved backbone folding by core residues, but using extensive sequence variations in the seven loop regions of naturally occurring human kds, we generated a synthetic kd library on the yeast cell surface by randomizing 45 residues in the loops of a human kd template. ... | 2010 | 20460308 |
| application of simple fed-batch technique to high-level secretory production of insulin precursor using pichia pastoris with subsequent purification and conversion to human insulin. | the prevalence of diabetes is predicted to rise significantly in the coming decades. a recent analysis projects that by the year 2030 there will be ~366 million diabetics around the world, leading to an increased demand for inexpensive insulin to make this life-saving drug also affordable for resource poor countries. | 2010 | 20462406 |
| ash2l interacts with tbx1 and is required during early embryogenesis. | tbx1 encodes a dna binding transcription factor that is commonly deleted in human digeorge syndrome and plays an important role in heart development. mechanisms of tbx1 function, such as tbx1 interacting regulatory proteins and transcriptional target specificity, are largely unknown. ash2l is the mammalian homolog of drosophila ash2 (absent small homeotic 2) and is a core component of a multimeric histone methyltransferase complex that epigenetically regulates transcription via methylation of hi ... | 2010 | 20463296 |
| blastomycosis: case report of an isolated lesion in the distal fibula. | blastomycosis (blastomyces dermatitidis) is a fungal infection that occurs primarily in the lungs, but 15% to 60% of patients with systemic blastomycosis have skeletal involvement. because the symptoms and radiographic appearance of bony lesions are variable, diagnosis and treatment may be delayed if fungal infections are not included in the differential diagnosis for a patient with a lytic bone lesion. we present the case of a man in his late 30s with no local or systemic signs of infection in ... | 2010 | 20463990 |
| prediction of functionally selective allosteric interactions at an m3 muscarinic acetylcholine receptor mutant using saccharomyces cerevisiae. | saccharomyces cerevisiae is a tractable yeast species for expression and coupling of heterologous g protein-coupled receptors with the endogenous pheromone response pathway. although this platform has been used for ligand screening, no studies have probed its ability to predict novel pharmacology and functional selectivity of allosteric ligands. as a proof of concept, we expressed a rat m(3) muscarinic acetylcholine receptor (machr) bearing a mutation (k(7.32)e) recently identified to confer pos ... | 2010 | 20466821 |
| a nuclear targeting system in plasmodium falciparum. | the distinct differences in gene control mechanisms acting in the nucleus between plasmodium falciparum and the human host could lead to new potential drug targets for anti-malarial development. new molecular toolkits are required for dissecting molecular machineries in the p. falciparum nucleus. one valuable tool commonly used in model organisms is protein targeting to specific sub-cellular locations. targeting proteins to specified locations allows labeling of organelles for microscopy, or tes ... | 2010 | 20470378 |
| antifungal activity, experimental infections and nail permeation of an innovative ciclopirox nail lacquer based on a water-soluble biopolymer. | p-3051 is an innovative 8% ciclopirox nail lacquer, based on hydroxypropyl chitosan (hpch) as a film-forming agent. the authors' aim was to investigate p-3051's in vitro antifungal activity, as well as its in vitro and in vivo nail permeation. the dilution susceptibility tests performed for trichophyton rubrum (t. rubrum) and candida parapsilosis (c. parapsilosis) showed that the minimum inhibitory concentrations (mics) of p-3051, as percent of ciclopirox, was for both fungi < or = 0.0015% (equi ... | 2010 | 20480796 |
| transcription factors mat2 and znf2 operate cellular circuits orchestrating opposite- and same-sex mating in cryptococcus neoformans. | cryptococcus neoformans is a human fungal pathogen that undergoes a dimorphic transition from a unicellular yeast to multicellular hyphae during opposite sex (mating) and unisexual reproduction (same-sex mating). opposite- and same-sex mating are induced by similar environmental conditions and involve many shared components, including the conserved pheromone sensing cpk1 mapk signal transduction cascade that governs the dimorphic switch in c. neoformans. however, the homeodomain cell identity pr ... | 2010 | 20485569 |
| candida dubliniensis screening using the germ tube test in clinical yeast isolates and prevalence of c. dubliniensis in korea. | the aim of this study was to screen for c. dubliniensis using the germ tube test with human pooled serum (hps) in clinical isolates and investigate the prevalence of c. dubliniensis in korea. among 1,854 yeast strains isolated, 1,404 strains of c. albicans (on the basis of positive results of the germ tube test) and 192 germ tube-negative yeast strains were examined. all 1,596 clinical isolates were examined using the germ tube test with hps, the differential temperature, and nacl tolerance test ... | 2010 | 20486193 |
| characterization of yeasts colonizing in healthy individuals. | opportunistic yeast pathogens may switch from harmless commensal to pathogenic relationships with the host under different conditions. they usually cause superficial infections, but may be the agents of more significant infections in immunocompromised patients. to investigate yeast colonization in the oral cavities of clinically healthy individuals, we collected oral swabs from 323 students and staff at the national health research institutes, taiwan. a total of 49 (15.2%) volunteers were coloni ... | 2011 | 20491531 |
| combination of sodium chlorite and calcium propionate reduces enzymatic browning and microbial population of fresh-cut "granny smith" apples. | tissue browning and microbial growth are the main concerns associated with fresh-cut apples. in this study, effects of sodium chlorite (sc) and calcium propionate (cp), individually and combined, on quality and microbial population of apple slices were investigated. "granny smith" apple slices, dipped for 5 min in cp solutions at 0%, 0.5%, 1%, and 2% (w/v) either alone or in combination with 0.05% (w/v) sc, were stored at 3 and 10 degrees c for up to 14 d. color, firmness, and microflora populat ... | 2010 | 20492244 |
| candida species isolated from the gastrointestinal tract of cockatiels (nymphicus hollandicus): in vitro antifungal susceptibility profile and phospholipase activity. | over the past years, the incidence of yeast infections, especially candidiasis, has increased. it is known that birds, including cockatiels, harbor potentially pathogenic yeasts to human beings in their gastrointestinal tract. thus, this work aims at determining the in vitro antifungal susceptibility and phospholipase activity of candida spp. isolated from the gastrointestinal tract and stools of cockatiels. sixty cockatiels were assessed and samples were collected from oral cavity, crop and clo ... | 2010 | 20493645 |
| transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides. | fusion proteins made up of glucagon-like peptide 1 (glp-1) and exendin-4 (ex-4) fused to a nonglycosylated form of human transferrin (glp-1-tf or ex-4-tf) were produced and characterized. glp-1-tf activated the glp-1 receptor, was resistant to inactivation by peptidases, and had a half-life of approximately 2 days, compared with 1 to 2 min for native glp-1. glp-1-tf retained the acute, glucose-dependent insulin-secretory properties of native glp-1 in diabetic animals and had a profound effect on ... | 2010 | 20498254 |
| upregulated expression of hitf in crohn's disease and screening of hitf interactant by a yeast two-hybrid system. | to study the expression of human intestinal trefoil factor (hitf) mrna in crohn's disease and to screen the cellular proteins that can interact with the hitf protein by a yeast two-hybrid system in order to explore the mechanism of hitf in protecting intestinal mucosa from injury. | 2010 | 20499178 |
| phosphate activated glutaminase-like immunoreactivity in the nervous system from different species and in different neuronal cell types and in astrocytes. | using antibodies against pig brain phosphate activated glutaminase, the enzyme appears to be rather conservative as we have observed immuno- staining in the brain from all species investegated [pig, cow, rabbit, rat, mouse, man, fish (cod and salmon) and bird (chicken)]. in addition, phosphate activated glutaminase from cultured mouse cerebral cortex inter- neurons (mainly gaba-ergic), cerebellar granule cells (glutamatergic) and astrocytes stained in an analogous manner. however, no phosphate a ... | 1987 | 20501086 |
| htlv-1 basic leucine-zipper factor, hbz, interacts with mafb and suppresses transcription through a maf recognition element. | htlv-1 infection causes adult t-cell leukemia (atl). the development of atl is thought to be associated with disruption of transcriptional control of cellular genes. htlv-1 basic leucine-zipper (bzip) factor, hbz, is encoded by the complementary strand of the provirus. we previously reported that hbz interacts with c-jun and suppresses its transcriptional activity. to identify the cellular factor(s) that interact with hbz, we conducted a yeast two-hybrid screen using full-length hbz as bait and ... | 2010 | 20506502 |
| identification of cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) as a novel alpha1a-adrenoceptor-down-regulating protein and establishment of an alpha1l-adrenoceptor-expressing cell line. | two distinct alpha(1)-adrenoceptor phenotypes (alpha(1a)- and alpha(1l)-ars) are known to originate from a single adra1a(alpha(1a)) gene by an as-yet-unknown mechanism. we hypothesized that an alpha(1a)-ar-interacting protein could generate the alpha(1l)-ar phenotype and we sought to identify such a protein and to examine its effects on the expression of alpha(1a) and alpha(1l) phenotypes. cysteine-rich epidermal growth factor-like domain 1alpha (creld1alpha) was first identified using a yeast t ... | 2010 | 20508391 |
| duplicability of self-interacting human genes. | there is increasing interest in the evolution of protein-protein interactions because this should ultimately be informative of the patterns of evolution of new protein functions within the cell. one model proposes that the evolution of new protein-protein interactions and protein complexes proceeds through the duplication of self-interacting genes. this model is supported by data from yeast. we examined the relationship between gene duplication and self-interaction in the human genome. | 2010 | 20509897 |
| struct2net: a web service to predict protein-protein interactions using a structure-based approach. | struct2net is a web server for predicting interactions between arbitrary protein pairs using a structure-based approach. prediction of protein-protein interactions (ppis) is a central area of interest and successful prediction would provide leads for experiments and drug design; however, the experimental coverage of the ppi interactome remains inadequate. we believe that struct2net is the first community-wide resource to provide structure-based ppi predictions that go beyond homology modeling. a ... | 2010 | 20513650 |
| plasticity of human protein disulfide isomerase: evidence for mobility around the x-linker region and its functional significance. | protein disulfide isomerase (pdi), which consists of multiple domains arranged as abb'xa'c, is a key enzyme responsible for oxidative folding in the endoplasmic reticulum. in this work we focus on the conformational plasticity of this enzyme. proteolysis of native human pdi (hpdi) by several proteases consistently targets sites in the c-terminal half of the molecule (x-linker and a' domain) leaving large fragments in which the n terminus is intact. fluorescence studies on the w111f/w390f mutant ... | 2010 | 20516074 |
| bat3 promotes the membrane integration of tail-anchored proteins. | the membrane integration of tail-anchored proteins at the endoplasmic reticulum (er) is post-translational, with different tail-anchored proteins exploiting distinct cytosolic factors. for example, mammalian trc40 has a well-defined role during delivery of tail-anchored proteins to the er. although its saccharomyces cerevisiae equivalent, get3, is known to function in concert with at least four other components, get1, get2, get4 and get5 (mdy2), the role of additional mammalian proteins during t ... | 2010 | 20516149 |
| the fanconi anemia protein, fancg, binds to the ercc1-xpf endonuclease via its tetratricopeptide repeats and the central domain of ercc1. | there is evidence that fanconi anemia (fa) proteins play an important role in the repair of dna interstrand cross-links (icls), but the precise mechanism by which this occurs is not clear. one of the critical steps in the icl repair process involves unhooking of the cross-link from dna by incisions on one strand on either side of the icl and its subsequent removal. the ercc1-xpf endonuclease is involved in this unhooking step and in the removal of the cross-link. we have previously shown that se ... | 2010 | 20518486 |
| [polar steroids from solaster endeca starfish and the physiological activity of polar steroids from three starfish species]. | four polyhydroxylated steroids, new (20r)-5alpha-cholestan-3beta,6alpha,8,15alpha,24,26-hexaol (i) and known (20r,25s)-5alpha-cholestan-3beta6alpha,8,15beta,16beta,26-hexaol, (20r,25s)-5alpha-cholestan-3beta,6alpha,15beta,16beta,26-pentaol, and marthasterone sulfate were isolated from the solaster endeca starfish inhabiting the sea of okhotsk and characterized. steroid (i) contains a 24,26-dihydroxylated side chain, which is uncommon for starfish polyols. the isolated steroids and related metabo ... | 2010 | 20531484 |
| molecular bridging of aging and cancer: a carf link. | collaborator of arf (carf) was first cloned as an arf partner in yeast two-hybrid screens. it enhances arf-dependent and -independent p53 functions, which are central to the control of cell growth and tumor suppression in human cells. carf interacts with arf, p53, and mdm2 proteins, and in turn gets regulated by mdm2-mediated degradation, suggesting a self-regulatory loop. carf is upregulated during replicative, oncogenic, and stress-induced senescence. overexpression of carf induced premature s ... | 2010 | 20536841 |
| the jmjn domain of jhd2 is important for its protein stability, and the plant homeodomain (phd) finger mediates its chromatin association independent of h3k4 methylation. | histone lysine methylation is a dynamic process that plays an important role in regulating chromatin structure and gene expression. recent studies have identified jhd2, a jmjc domain-containing protein, as an h3k4-specific demethylase in budding yeast. however, important questions regarding the regulation and functions of jhd2 remain unanswered. in this study, we show that jhd2 has intrinsic activity to remove all three states of h3k4 methylation in vivo and can dynamically associate with chroma ... | 2010 | 20538609 |
| predicting genetic modifier loci using functional gene networks. | most phenotypes are genetically complex, with contributions from mutations in many different genes. mutations in more than one gene can combine synergistically to cause phenotypic change, and systematic studies in model organisms show that these genetic interactions are pervasive. however, in human association studies such nonadditive genetic interactions are very difficult to identify because of a lack of statistical power--simply put, the number of potential interactions is too vast. one appro ... | 2010 | 20538624 |
| nutrient regulation of mtorc1 and cell growth. | | 2010 | 20543582 |
| the c-terminal alpha-alpha superhelix of pat is required for mrna decapping in metazoa. | pat proteins regulate the transition of mrnas from a state that is translationally active to one that is repressed, committing targeted mrnas to degradation. pat proteins contain a conserved n-terminal sequence, a proline-rich region, a mid domain and a c-terminal domain (pat-c). we show that pat-c is essential for the interaction with mrna decapping factors (i.e. dcp2, edc4 and lsm1-7), whereas the p-rich region and mid domain have distinct functions in modulating these interactions. dcp2 and e ... | 2010 | 20543818 |
| alpha4 phosphoprotein interacts with edd e3 ubiquitin ligase and poly(a)-binding protein. | mammalian alpha4 phosphoprotein, the homolog of yeast tap42, is a component of the mammalian target-of-rapamycin (mtor) pathway that regulates ribogenesis, the initiation of translation, and cell-cycle progression. alpha4 is known to interact with the catalytic subunit of protein phosphatase 2a (pp2ac) and to regulate pp2a activity. using alpha4 as bait in yeast two-hybrid screening of a human k562 erythroleukemia cdna library, edd (e3 isolated by differential display) e3 ubiquitin ligase was id ... | 2010 | 20544796 |
| expression, purification and characterization of the cancer-germline antigen gage12i: a candidate for cancer immunotherapy. | gage cancer-germline antigens are frequently expressed in a broad range of different cancers, while their expression in normal tissues is limited to the germ cells of the immune privileged organs, testis and ovary. gage proteins are immunogenic in humans, which make them promising targets for immunotherapy and candidates for cancer vaccines. recombinant proteins may be superior to peptides as immunogens, since they have the potential to prime both cd4(+) and cd8(+) t cells and are not dependent ... | 2010 | 20546897 |
| the production, characterisation and enhanced pharmacokinetics of scfv-albumin fusions expressed in saccharomyces cerevisiae. | an expression system is described for the production of monomeric scfvs and scfv antibody fragments genetically fused to human albumin (at either the n- or c-terminus or both). based upon strains of saccharomyces cerevisiae originally developed for the production of a recombinant human albumin (recombumin) this system has delivered high levels of secreted product into the supernatant of shake flask and high cell density fed-batch fermentations. specific binding to the corresponding ligand was de ... | 2010 | 20546898 |
| the sarcomeric z-disc component myopodin is a multiadapter protein that interacts with filamin and alpha-actinin. | here we introduce myopodin as a novel filamin c binding partner. corroborative yeast two-hybrid and biochemical analyses indicate that the central part of myopodin that shows high homology to the closely related protein synaptopodin and that is common to all its currently known or predicted variants interacts with filamin c immunoglobulin-like domains 20-21. a detailed characterization of the previously described interaction between myopodin and alpha-actinin demonstrates for the first time that ... | 2010 | 20554076 |
| intramembrane proteolysis of mgm1 by the mitochondrial rhomboid protease is highly promiscuous regarding the sequence of the cleaved hydrophobic segment. | rhomboids are a family of intramembrane serine proteases that are conserved in bacteria, archaea, and eukaryotes. they are required for numerous fundamental cellular functions such as quorum sensing, cell signaling, and mitochondrial dynamics. mitochondrial rhomboids form an evolutionarily distinct class of rhomboids. it is largely unclear how their activity is controlled and which substrate determinants are responsible for recognition and cleavage. we investigated these requirements for the mit ... | 2010 | 20558178 |
| [lass2 interacts with v-atpase and inhibits cell growth of hepatocellular carcinoma]. | homo sapiens longevity assurance homologue 2 (lass2) is a novel gene isolated from a human liver cdna library by our laboratory, and it is a human homologue of the yeast longevity assurance gene lag1 (saccharomyces cerevisiae longevity assurance gene). according to our previous results, lass2 could interact with subunit c of vacuolar type h(+)-atpase (v-atpase), and the overexpression of lass2 could inhibit the cell growth of a human hepatocellular carcinoma (hcc) cell line, smmc-7721. in order ... | 2010 | 20571735 |
| streptococcus mutans inhibits candida albicans hyphal formation by the fatty acid signaling molecule trans-2-decenoic acid (sdsf). | in the human mouth, fungi and several hundred species of bacteria coexist. here we report a case of interkingdom signaling in the oral cavity: a compound excreted by the caries bacterium streptococcus mutans inhibits the morphological transition from yeast to hyphae, an important virulence trait, in the opportunistic fungus candida albicans. the compound excreted by s. mutans was originally studied because it inhibited signaling by the universal bacterial signal autoinducer-2 (ai-2), determined ... | 2010 | 20572249 |
| the cellular rna helicase ddx1 interacts with coronavirus nonstructural protein 14 and enhances viral replication. | the involvement of host proteins in the replication and transcription of viral rna is a poorly understood area for many rna viruses. for coronaviruses, it was long speculated that replication of the giant rna genome and transcription of multiple subgenomic mrna species by a unique discontinuous transcription mechanism may require host cofactors. to search for such cellular proteins, yeast two-hybrid screening was carried out by using the nonstructural protein 14 (nsp14) from the coronavirus infe ... | 2010 | 20573827 |
| the closely related rna helicases, uap56 and urh49, preferentially form distinct mrna export machineries and coordinately regulate mitotic progression. | nuclear export of mrna is an essential process for eukaryotic gene expression. the trex complex couples gene expression from transcription and splicing to mrna export. sub2, a core component of the trex complex in yeast, has diversified in humans to two closely related rna helicases, uap56 and urh49. here, we show that urh49 forms a novel urh49-cip29 complex, termed the arex (alternative mrna export) complex, whereas uap56 forms the human trex complex. the mrnas regulated by these helicases are ... | 2010 | 20573985 |
| estrogenic pyrethroid pesticides regulate expression of estrogen receptor transcripts in mouse sertoli cells differently from 17beta-estradiol. | studies suggested that exposure to agricultural pesticides may affect male fertility. pyrethroids are widely used pesticides due to their insecticidal potency and low mammalian toxicity. a recombinant yeast assay system incorporating the human alpha-estrogen receptor was used to analyze the estrogenicity of a range of readily available pyrethroid pesticides. the commercial product ripcord plus showed estrogenic activity by this assay. to determine whether pyrethroid compounds might exert an effe ... | 2010 | 20574910 |
| long-term conservation of hcv rna at 4 degrees c using a new rna stabilizing solution. | protecting rna from degradation, whilst maintaining its biological activity, is essential in molecular biology. however, rna is very sensitive to degradation by ribonucleases, especially at temperatures above 0 degrees c. the stability of rna was examined at 4 degrees c and -20 degrees c, in a new stabilizing solution consisting of a low-molarity mixture of chaotropic agents guanidinium and ammonium thiocyanate, a buffer for ph stabilization, phenol, and yeast rna. two substrates were tested for ... | 2010 | 20576506 |
| the genemania prediction server: biological network integration for gene prioritization and predicting gene function. | genemania (http://www.genemania.org) is a flexible, user-friendly web interface for generating hypotheses about gene function, analyzing gene lists and prioritizing genes for functional assays. given a query list, genemania extends the list with functionally similar genes that it identifies using available genomics and proteomics data. genemania also reports weights that indicate the predictive value of each selected data set for the query. six organisms are currently supported (arabidopsis thal ... | 2010 | 20576703 |
| simple is good: yeast models of neurodegeneration. | the budding yeast, saccharomyces cerevisiae, is the best-studied eukaryotic cell, at both genetic and physiological levels. as a eukaryote, yeast shares highly conserved molecular and cellular mechanisms with human cells. thus, this simple fungus is an invaluable model to study the fundamental molecular mechanisms involved in several human diseases. in the particular case of neurodegenerative disorders, yeast models have been able to recapitulate several important features of complex and devasta ... | 2010 | 20579105 |
| recombinant form of human wild type mannan-binding lectin (mbl/a) but not its structural variant (mbl/c) promotes phagocytosis of zymosan by activating complement. | mannan-binding lectin (mbl) mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. in the present study, employing recombinant forms of human mbl (rmbl), the role of wild type mbl (rmbl/a) and its structural variant rmbl/c in mediating thp-1 phagocytosis of fluorescent-labeled zymosan was examined and compared to mbl purified from human plasma (pmbl/a). flow cytometric analyses revealed that opsonization of zymosan with r ... | 2010 | 20579738 |
| antimicrobial activity of mucosal-associated invariant t cells. | mucosal-associated invariant t lymphocytes (mait lymphocytes) are characterized by two evolutionarily conserved features: an invariant t cell antigen receptor (tcr) alpha-chain and restriction by the major histocompatibility complex (mhc)-related protein mr1. here we show that mait cells were activated by cells infected with various strains of bacteria and yeast, but not cells infected with virus, in both humans and mice. this activation required cognate interaction between the invariant tcr and ... | 2010 | 20581831 |