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infections in patients with aplastic anemia.infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ...200919549579
evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts.flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ...200919553175
report: antibiotic production by thermophilic bacillus specie sat-4.production of antimicrobial compounds seems to be a general phenomenon for most bacteria. the prevalence of antimicrobial resistance among key microbial pathogens is increasing at an alarming rate worldwide. current solutions involve development of a more rationale approach to antibiotic use and discover of new antimicrobials. bacillus species produce a large number of biological compounds active against bacteria, fungi, protozoa and viruses. the process of production usually involves screening ...200919553186
effects of stingless bee and honey bee propolis on four species of bacteria.we examined the antibacterial activities of several types of propolis, including africanized honey bee green propolis and propolis produced by meliponini bees. the antibacterial activity of green propolis against micrococcus luteus and staphylococcus aureus was superior to that of melipona quadrifasciata and scaptotrigona sp propolis. only two samples of propolis (green propolis and scaptotrigona sp propolis) were efficient against escherichia coli. melipona quadrifasciata propolis was better th ...200919554760
uptake of the necrotic serpin in drosophila melanogaster via the lipophorin receptor-1.the humoral response to fungal and gram-positive infections is regulated by the serpin-family inhibitor, necrotic. following immune-challenge, a proteolytic cascade is activated which signals through the toll receptor. toll activation results in a range of antibiotic peptides being synthesised in the fat-body and exported to the haemolymph. as with mammalian serpins, necrotic turnover in drosophila is rapid. this serpin is synthesised in the fat-body, but its site of degradation has been unclear ...200919557185
antibacterial activity of recombinant hcap18/ll37 protein secreted from pichia pastoris.human antimicrobial peptide cap18/ll37 (hcap18/ll37) was expressed in pichia pastoris and its antibacterial activity was tested against pathogenic bacteria. the full length orf of hcap18/ll37 was cloned into the ppiczaa vector followed by integration into the genomic aox1 gene of p. pastoris. agar diffusion assay demonstrated that the different hcap18/ll37 transformants showed various antibacterial activities against staphylococcus aureus, micrococcus luteus, and salmonella gastroenteritis. the ...200919557354
the hydrophobicity in a chemically modified side-chain of cysteine residues of thanatin is related to antimicrobial activity against micrococcus luteus.the chemically modified thanatins with the methyl group (ch(3)), ethyl group (c(2)h(5)), and normal-octyl group (c(8)h(17)) at the side-chain of cysteine residues were synthesized. the octyl group modified form exhibited 8-fold higher antimicrobial activity against micrococcus luteus than wild type thanatin. it was found that there was an equilateral correlation between antimicrobial activity and side-chain hydrophobicity at the cysteine residues in thanatin.200919584529
anti-listerial activity and structure-activity relationships of the six major tyrocidines, cyclic decapeptides from bacillus aneurinolyticus.six major tyrocidines, purified from the antibiotic tyrothricin complex produced by bacillus aneurinolyticus, showed significant lytic and growth inhibitory activity towards the gram+ bacteria, micrococcus luteus and listeria monocytogenes, but not against the gram- bacterium, escherichia coli. the isolated natural tyrocidines were in particular more active against the leucocin a (antimicrobial peptide) resistant strain, l. monocytogenes b73-mr1, than the sensitive l. monocytogenes b73 strain. r ...200919586775
micronization of lysozyme by supercritical assisted atomization.supercritical assisted atomization (saa) has been used to produce lysozyme microparticles. lysozyme has been micronized using water, buffered water at ph 6.2 and water-ethanol mixtures at different volume percentages. precipitated lysozyme particles were spherical, with a narrow particle size distribution (psd) ranging from 0.1 to 4 microm. the concentration of lysozyme in the liquid solvent mixture had a nonlinear effect on the particle distribution, with an increase of the x(0.9) from about 1 ...200919603424
micrococcus yunnanensis sp. nov., a novel actinobacterium isolated from surface-sterilized polyspora axillaris roots.in this study, strain yim 65004(t), isolated from roots of polyspora axillaris, was shown to represent a novel species of the genus micrococcus by means of a polyphasic approach. chemotaxonomic data gathered for peptidoglycan type, menaquinones, phospholipids and fatty acids strongly supported the classification of this strain within the genus micrococcus: the cell-wall peptidoglycan contained lysine, glutamic acid, alanine, glycine and aspartic acid, the predominant menaquinones were mk-8(h(2)) ...200919620366
[peritonitis due to kocuria rosea in a continuous ambulatory peritoneal dialysis case].micrococcus strains which are the normal flora members of skin, mucosa and oropharynx, may lead to infections associated with intravenous catheter, chronic ambulatory peritoneal dialysis, venticular shunt and prosthetic valve. in this paper, a case of peritonitis due to kocuria rosea of micrococcea family, in a patient undergoing continuous ambulatory peritoneal dialysis (capd), was presented. fiftysix years old female patient was admitted to the hospital by complaints of abdominal pain, nausea ...200919621623
lyngbyazothrins a-d, antimicrobial cyclic undecapeptides from the cultured cyanobacterium lyngbya sp.four novel cyclic undecapeptides, lyngbyazothrins a (1), b (2), c (3), and d (4), were isolated from the cultured lyngbya sp. 36.91 as binary mixtures (1/2 and 3/4). their structures were elucidated by analysis of 1d (1h and 13c) and 2d (cosy, tocsy, roesy, noesy, hmqc, and hmbc) nmr spectra, esimsms, esitofms, and amino acid analyses. three unusual amino acids were present and identified as 4-methoxyhomophenylalanine in 1 and 3, homophenylalanine in 2 and 4, and 3-amino-2,5,7,8-tetrahydroxy-10- ...200919621877
endophytic bacteria associated with growing shoot tips of banana (musa sp.) cv. grand naine and the affinity of endophytes to the host.a cultivation-based assessment of endophytic bacteria present in deep-seated shoot tips of banana suckers was made with a view to generate information on the associated organisms, potential endophytic contaminants in tissue-cultured bananas and to assess if the endophytes shared a beneficial relationship with the host. plating the tissue homogenate from the central core of suckers showed colony growth on nutrient agar from just 75% and 42% of the 12 stocks during may and november, respectively ( ...200919633807
biocompatible ionic liquids: a new approach for stabilizing proteins in liquid formulation.ionic liquids (ils) have shown excellent promise as both solutes and solvents for stabilizing proteins at room temperature. because many modern drugs are protein-based, these stabilizing characteristics have great potential to provide advances in the field of liquid formulation of therapeutic proteins. however, before these developments can be translated into clinical solutions it is essential to establish data related to the biocompatibility of these ils. the current work investigates the cytot ...200919640150
structure and applications of a rhamnolipid surfactant produced in soybean oil waste.soybean oil soapstock was utilized as an alternative carbon source for the production of rhamnolipids by pseudomonas aeruginosa lbi strain. the chemical composition and properties of the rhamnolipid mixture obtained were determined to define its potential applications. the chemical characterization of the rhamnolipid has revealed the presence of ten different homologues. the monorhamnolipid rhac10c1) and the dirhamnolipid rha2c10c10 were the main components of the mixture that showed predominanc ...201019649781
does the microbial flora in the ejaculate affect the freezeability of stallion sperm?in an attempt to evaluate the possible relationship between the microbial flora in the stallion ejaculate and its ability to freeze,three ejaculates from five stallions were frozen using a standard protocol. before freezing, an aliquot was removed for bacteriological analysis. bacterial growth was observed in all the ejaculates studied. the isolated microorganisms were:staphylococcus spp. and micrococcus spp. (in all the stallions), beta-haemolytic streptococcus (in stallions 3 and 4), corynebac ...200919655428
synthesis and bioactivity of poly(hpma)-lysozyme conjugates: the use of novel thiazolidine-2-thione coupling chemistry.a novel thiazolidine-2-thione functionalized chain transfer agent (cta) was synthesized and used as a reversible addition-fragmentation chain transfer (raft) polymerization agent to prepare well-defined poly-n-(2-hydroxypropyl) methacrylamide (phpma). the polymer chains had pre-designed molecular weights, narrow polydispersities and were chain-end functionalized. on incubation with protein (lysozyme) under different ph conditions, phpma was conjugated to the protein surface via covalent amide bo ...200919675903
genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium xenorhabdus nematophila.xenocoumacin 1 (xcn1) and xenocoumacin 2 (xcn2) are the major antimicrobial compounds produced by xenorhabdus nematophila. to study the role of xcn1 and xcn2 in the life cycle of x. nematophila the 14 gene cluster (xcna-n) required for their synthesis was identified. overlap rt-pcr analysis identified six major xcn transcripts. individual inactivation of the non-ribosomal peptide synthetase genes, xcna and xcnk, and polyketide synthetase genes, xcnf, xcnh and xcnl, eliminated xcn1 production. xc ...200919682255
the involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis.the suppressors of cytokine signaling 2 (socs2) has been identified as negative feedback inhibitors for various cytokines signaling via the jak/stat pathway. in the present studies, the cdna of eriocheir sinensis socs2 (designated as essocs2) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) approaches. the full-length cdna of essocs2 was of 2535bp, consisting of an open reading frame (a orf) of 1071bp encoding a polypeptide of 357 amino acids. the deduced am ...201019686773
bacteria with dual resistance to elevated concentrations of heavy metals and antibiotics in nigerian contaminated systems.samples of soil, water, and sediments from industrial estates in lagos were collected and analyzed for heavy metals and physicochemical composition. bacteria that are resistant to elevated concentrations of metals (cd(2+), co(2+), ni(2+), cr(6+), and hg(2+)) were isolated from the samples, and they were further screened for antibiotic sensitivity. the minimum tolerance concentrations (mtcs) of the isolates with dual resistance to the metals were determined. the physicochemistry of all the sample ...201019688604
antibacterial potential of antagonistic streptomyces sp. isolated from marine sponge dendrilla nigra.the role of streptomyces sp. (btl7) in synthesis of antibacterial agents reported from the marine sponge dendrilla nigra was evaluated. selective isolation of actinomycetes was performed on the newly developed selective media, sponge agar (sa) 1 and sa 2. the growth rate and antibiotic production were increased on the media supplemented with sponge extract. the chosen isolate btl7 showed inhibitory interaction with micrococcus luteus and the extracellular products contained potent antibacterial ...200419712370
[mechanisms of protective action of bacterial intracellular metabolites for lowering of bactericidal effect of hydroxyl radicals].to study mechanisms of attenuation of bactericidal effect of hydroxyl radicals by bacterial intracellular metabolites.200919715199
distribution and diversity of a cadmium resistance (cada) determinant and occurrence of is257 insertion sequences in staphylococcal bacteria isolated from a contaminated estuary (seine, france).water and sediments of the seine estuary are contaminated by chemicals, especially cadmium, which favors survival and growth of cadmium-resistant bacteria. we investigated the diversity of the cada gene, which encodes a cd(2+)/atpase protein transporter, in the microbial community of the seine estuary. the cada gene first isolated from s. aureus (pi258) was prevalent, with a conservation better than 98% identity, despite its presence in host bacteria of diverse species and genera. we report for ...200319719677
metabolism of azo dyes by human skin microbiota.reduction of methyl red (mr) and orange ii (or ii) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. the analysis indicated that skin bacteria, representing the genera staphylococcus, corynebacterium, micrococcus, dermacoccus and kocuria, were able to reduce mr by 74-100 % in 24 h, with only three species unable to reduce completely the dye in that time. among the species tested, only corynebacterium xerosis was unable to reduce or ii to any degree by 24 h, an ...201019729456
structural changes and cellular localization of resuscitation-promoting factor in environmental isolates of micrococcus luteus.dormancy among nonsporulating actinobacteria is now a widely accepted phenomenon. in micrococcus luteus, the resuscitation of dormant cells is caused by a small secreted protein (resuscitation-promoting factor, or rpf) that is found in "spent culture medium." rpf is encoded by a single essential gene in m. luteus. homologs of rpf are widespread among the high g + c gram-positive bacteria, including mycobacteria and streptomycetes, and most organisms make several functionally redundant proteins. ...201019730766
chemical composition, antifungal and antibacterial activity of the essential oil of chamaecyparis nootkatensis from spain.the chemical composition of the oil of chamaecyparis nootkatensis (d. don) spach. has been analysed by gas chromatography (gc) and gas chromatography coupled to mass spectrometry (gc-ms). a total of 34 compounds were identified representing more than 90% of the total oil. the oil was richer in monoterpenes than in sesquiterpenes, the major constituents being limonene (53.2%), 6-3-carene (21.0%) and a-pinene (12.2%). the antibacterial and antifungal activities of the oil were also tested against ...200919731613
dielectrophoretic separation of airborne microbes and dust particles using a microfluidic channel for real-time bioaerosol monitoring.airborne microbes such as fungi, bacteria, and viruses are a threat to public health. robust and real-time detection systems are necessary to prevent and control such dangerous biological particles in public places and dwellings. for direct and real-time detection of airborne microbes, samples must be collected and typically resuspended in liquid prior to detection; however, environmental particles such as dust are also trapped in such samples. therefore, the isolation of target bacteria or a se ...200919731688
nitric oxide is an essential component of the hemocyte-mediated mosquito immune response against bacteria.nitric oxide is a signaling and immune effector molecule synthesized by the enzyme nitric oxide synthase. in mosquitoes, nitric oxide functions as a parasite antagonist in the midgut but little is known about its function in the hemocoel. here, we characterized the temporal and spatial expression of the anopheles gambiae nitric oxide synthase gene and explored the role nitric oxide plays in the antibacterial response in the mosquito hemocoel. quantitative pcr and western blot analyses showed tha ...201019733588
functional recognition imaging using artificial neural networks: applications to rapid cellular identification via broadband electromechanical response.functional recognition imaging in scanning probe microscopy (spm) using artificial neural network identification is demonstrated. this approach utilizes statistical analysis of complex spm responses at a single spatial location to identify the target behavior, which is reminiscent of associative thinking in the human brain, obviating the need for analytical models. we demonstrate, as an example of recognition imaging, rapid identification of cellular organisms using the difference in electromech ...200919752493
comparison of the bacterial community and characterization of plant growth-promoting rhizobacteria from different genotypes of chrysopogon zizanioides (l.) roberty (vetiver) rhizospheres.molecular approaches [pcr-denaturing gradient gel electrophoresis (dgge)] were used to determine whether three different vetiver (chrysopogon zizanioides) genotypes, commercially used in brazil and considered economically important over the world, select specific bacterial populations to coexist in their rhizospheres. dgge profiles revealed that the predominant rhizospheric bacterial community hardly varies regarding the vetiver genotype. moreover, using traditional cultivation methods, bacteria ...200919763409
microwave assisted, one-pot synthesis of 5-nitro- 2-aryl substituted-1h-benzimidazole libraries: screening in vitro for antimicrobial activity.the efficient and rapid synthesis of 5-nitro-2-aryl substituted-1h-benzimidazole libraries (1a-1j) has been established. thus, both microwave and conventional cyclo-condensation of 4-nitro ortho-phenylenediamine with various phenoxyacetic acids were carried out in the presence of hcl catalyst. the microwave synthesis route afforded advantages, such as good to excellent yields, shorter reaction time (2.5-3.5 min), readily available starting material, and simple purification procedure, which disti ...200919772484
antibacterial activity of oregano (origanum vulgare linn.) against gram positive bacteria.the present investigation is focused on antibacterial potential of infusion, decoction and essential oil of oregano (origanum vulgare) against 111 gram-positive bacterial isolates belonging to 23 different species related to 3 genera. infusion and essential oil exhibited antibacterial activity against staphylococcus saprophyticus, s. aureus, micrococcus roseus, m. kristinae, m. nishinomiyaensis, m. lylae, m. luteus, m. sedentarius, m. varians, bacillus megaterium, b. thuringiensis, b. alvei, b. ...200919783523
agrococcus terreus sp. nov. and micrococcus terreus sp. nov., isolated from forest soil.two bacterial strains, dng5t and v3m1t, isolated from forest soil of the changbai mountains in china, were characterized using a polyphasic approach. analysis of their 16s rrna gene sequences indicated that strains dng5t and v3m1t were phylogenetically related to members of the genus agrococcus (96.0-98.4% similarity) and micrococcus (96.7-98.0% similarity), respectively, within the order actinomycetales. strains dng5t and v3m1t were gram-stain-positive and strictly aerobic and formed yellow col ...201019783614
correlation of nosocomial bloodstream infection incidences: an ecological study.this study aimed to correlate nosocomial bloodstream infections (nbis) across time against the ecological effect of infection control activities. all patients hospitalised >or=48h in the haematology and intensive care departments of a university hospital and discharged between 1 january 2004 and 30 june 2006 were prospectively included. the case definition of nbi infection was: (1) at least one positive blood culture justified by clinical signs, or (2) at least two positive blood cultures when t ...200919788948
the identification of a low molecular mass bacteriocin, rhamnosin a, produced by lactobacillus rhamnosus strain 68.this study focuses on the isolation and characterization of a peptide with bacteriocin-like properties isolated from lactobacillus rhamnosus strain 68, previously identified by 16s rrna gene sequencing and originating from human gastrointestinal flora.200919796123
6-hydroxymethyl-1-phenazine-carboxamide and 1,6-phenazinedimethanol from a marine bacterium, brevibacterium sp. kmd 003, associated with marine purple vase sponge.two new antibacterial phenazines were isolated from the culture broth of brevibacterium sp. kmd 003 obtained from a marine purple vase sponge of the genus callyspongia, collected in kyeongpo, gangwondo, korea. the structures of these compounds were determined to be 6-hydroxymethyl-1-phenazine-carboxamide (1) and 1,6-phenazinedimethanol (2) through analyses of hr-ei-ms and nmr data. compounds 1 and 2 showed antibacterial activities against enterococcus hirae and micrococcus luteus with 5 microm m ...200919798118
[optimization of fermentation conditions for cold-adapted amylase production by micrococcus antarcticus and its enzymatic properties].by single factor experiments, the fermentation conditions for cold-adapted amylase production from micrococcus antarcticus were determined as follows(medium g/l): na2 hpo4 2.0, kh2po4 1.0, mgso4 x 7h2o 0.1, nacl 5.0, (nh4)2so4 2.5, maltose 5.0, trace element solution 5.0 ml, ph 8.0, 100 ml/erlenmeyer flask (500 ml); cultivation was in a rotating shaker at 12 degrees c and 160 r/min for 64 h.under those conditions,the highest total enzyme activity (2.6 u/ml) was obtained and increased by 10.8 fol ...200919799319
immune parameters in the humoral fluids of amphioxus branchiostoma belcheri challenged with vibrio alginolyticus.the knowledge concerning the humoral immunity is scarce in amphioxus branchiostoma belcheri. this study measured the humoral parameters including lysozyme, antimicrobial activity, microbial agglutinin and haemagglutinin in amphioxus humoral fluids before and after vibrio alginolyticus challenge. after challenged with v. alginolyticus, the lysozyme activity, growth inhibiting activities against escherichia coli and v. alginolyticus and microbial agglutinating activities against micrococcus lysode ...201019800008
alteration of bacterial cell activity results in element composition change. 200919817137
static magnetic field exposure fails to affect the viability of different bacteria strains.the viability of the microbes saccharomyces cerevisiae, bacillus circulans, escherichia coli, micrococcus luteus, pseudomonas fluorescens, salmonella enteritidis, serratia marcescens, and staphylococcus aureus was tested under static magnetic field exposure up to 24 h in either a homogeneous (159.2 +/- 13.4 mt) or three types of inhomogeneous static magnetic fields: (i) peak-to-peak magnetic flux density 476.7 +/- 0.1 mt with a lateral magnetic flux density gradient of 47.7 t/m, (ii) 12.0 +/- 0. ...201019821495
detection and hazard assessment of pathogenic microorganisms in medical wastes.this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ...200919827491
[dormant form of micrococcus luteus and arthrobacter globiformis, not growing on standard media]. 200919827710
lysozymes and lysozyme-like proteins from the fall armyworm, spodoptera frugiperda.lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. by searching an est database from the fall armyworm, spodoptera frugiperda (negre et al., 2006), we identified five sequences encoding proteins of the lysozyme family. the deduced protein sequences corresponded to three classical c-type lysozymes sf-lys1, sf-lys2 and sf-lys3, and two lysozyme-like proteins, sf-llp1 and sf-llp2. sf ...200919828200
structure and mode of action of microplusin, a copper ii-chelating antimicrobial peptide from the cattle tick rhipicephalus (boophilus) microplus.microplusin, a rhipicephalus (boophilus) microplus antimicrobial peptide (amp) is the first fully characterized member of a new family of cysteine-rich amps with histidine-rich regions at the n and c termini. in the tick, microplusin belongs to the arsenal of innate defense molecules active against bacteria and fungi. here we describe the nmr solution structure of microplusin and demonstrate that the protein binds copper ii and iron ii. structured as a single alpha-helical globular domain, micro ...200919828445
arsenite tolerance and biotransformation potential in estuarine bacteria.bacterial isolates from water and sediment samples from freshwater, estuarine and marine regions were tested for their growth in the presence of different concentrations of arsenic. despite the generation times being longer in case of all bacterial isolates tested in nutrient broth with 200 ppm arsenite (as(3+)), many of them were able to attain log phase and substantial growth variously between 36 and 96 h. the isolates tolerating >or=200 ppm arsenic (as) were found to belong to enterobacteriac ...201019834801
hydroxytyrosol from tyrosol using hydroxyphenylacetic acid-induced bacterial cultures and evidence of the role of 4-hpa 3-hydroxylase.hydroxytyrosol (htyr) is a potent natural antioxidant found in olive mill wastewaters. bacterial conversion of 4-tyrosol (2-(4-hydroxyphenyl)-ethanol) to htyr was reported in a limited number of bacterial species including pseudomonas aeruginosa. in this work, we studied this conversion, taking as a model the newly isolated halomonas sp. strain htb24. it was first hypothesized that the enzyme responsible for 4-tyrosol hydroxylation in htyr was a 4-hydroxyphenylacetic acid 3-hydroxylase (hpah, ec ...200919837158
first report of linear megaplasmids in the genus micrococcus.high-altitude wetlands (above 4200m) in the northwest of argentina are considered pristine and extreme environments. micrococcus sp. a1, h5, and v7, isolated from such environments, were shown to contain linear megaplasmids, designated plma1, plmh5, and plmv7, respectively. as known from linear plasmids of other actinomycetes, all three plasmids were resistant to lambda exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5' dna ends. electrophore ...201019850076
moraxella catarrhalis bacteraemia associated with prosthetic vascular graft infection.moraxella catarrhalis, formerly called branhamella catarrhalis, 'neisseria catarrhalis' or 'micrococcus catarrhalis', is a gram-negative, aerobic diplococcus frequently found as a colonizer of the upper respiratory tract. over the last 20-30 years, this bacterium has emerged as a genuine pathogen, and is now considered an important cause of otitis media in children and an aetiological agent in pneumonia in adults with chronic obstructive pulmonary disease. however, bacteraemia due to m. catarrha ...201019850707
cloning and functional characterization of a new antimicrobial peptide gene stct1 from the venom of the scorpion scorpiops tibetanus.scorpion has an innovative venom gland, which is an important determinant in contributing to its successful survival for more than 400 million years. scorpion venom contains a diversity of bioactive peptides, which represent a tremendous hitherto unexplored resource for use in drug design and development. here, stct1, a new antimicrobial peptide gene, was screened and isolated from the venomous gland cdna library of the scorpion scorpiops tibetanus. the full-length cdna of stct1 is 369 nucleotid ...201019854232
comprehensive characterization of indoor airborne bacterial profile.this is the first detailed characterization of the airborne bacterial profiles in indoor environments. two restaurants were selected for this study. fifteen genera of bacteria were isolated from each restaurant and identified by three different bacterial identification systems including midi, biolog and riboprinter. the dominant bacteria of both restaurants were gram-positive bacteria in which micrococcus and bacillus species were the most abundant. most bacteria identified were representative s ...200919862931
a case of acute endocarditis caused by micrococcus zymogenes (nov. spec.), with a description of the microorganism.from a case of acute endocarditis of the aortic and mitral valves with infarctions m the spleen and kidneys a micrococcus was twice isolated in pure culture from the blood during life and was demonstrated after death both microscopically and in pure culture in large numbers in the valvular vegetations, the infarctions and other parts. no other species of microorganism was found. this micrococcus is very small, occurs mainly in pairs, sometimes in short chains, stains by gram's method, grows in s ...189919866921
a contribution to the bacteriology of rheumatic fever.the conclusions i would draw from this work are merely those stated in a former paper. 1. the results obtained by injections of streptococci are different from those produced by micrococcus rheumaticus. 2. micrococcus rheumaticus cannot be regarded as an attenuated streptococcus, nor acute rheumatism as an attenuated streptococcal pyaemia. 3. in uncomplicated cases of acute rheumatism the organism may not be found in the blood or in the joint exudates.190719867081
the bacteriology of sputum in common non-tuberculous infections of the upper and lower respiratory tracts, with special reference to lobar and broncho-pneumonia.1. in our examinations, only 38 per cent. of the infections of the respiratory tract below the glottis were pure, and this percentage was reached only by carefully following kitasato's method of handling sputa. 2. lobar pneumonia may produce sputum free from pneumococci, and may undoubtedly be caused by organisms other than thepneumococcus. 3. there is found a marked yearly variation in the organisms which excite inflammation of the respiratory tract. 4. micrococcus catarrhalis is usually consid ...191119867447
a study of cultures from sputum and blood in lobar pneumonia.in the thirty-two unselected cases studied, cultures were taken from both the blood and the sputum, and in five of these more than one blood culture was taken. in one case the culture was taken from the blood only, and in this instance the pneumococcus was found. this case will be considered only with reference to the incidence of the pneumococci in blood cultures in lobar pneumonia. the results in the thirty-two cases in which cultures were made both from the blood and the sputum are as follows ...191319867641
experimental arthritis in the rabbit, produced with streptococcus mitis.1. we have produced arthritis in 50 per cent. of the rabbits injected with streptococcus mitis. 2. the character of the arthritis is identical with that produced by micrococcus rheumaticus. 3. the exudate in and about the joints partakes of the same nature as that caused by streptococcus rheumaticus. 4. bacteriological studies show that streptococcus mitis can be recovered from about one third of the affected joints. 5. arthritis produced by other types of streptococci differs by reason of great ...191419867784
the reactions between bacteria and animal tissues under conditions of artificial cultivation.this report gives an outline of the results of observations on over 1,100 tissue cultures made during the fall, winter, and spring of 1913 to 1914. the work has been resumed in the fall of 1914 and will be continued along the same and allied lines, confirming the above results with other strains of the same organisms and with other bacteria and bacterial products. tests should be made with tissue and plasma from other animals, and the known pathogenicity of the organism for the animal and tissue ...191519867854
the reactions between bacteria and animal tissues under conditions of artificial cultivation : iii. the action of bacterial vaccines on tissue cultures in vitro.these results seem to indicate that many bacteria may be utilized by tissue cells as food for growth or may contain a substance or substances stimulating cell growth or multiplication. this substance is stable and is not destroyed by heating to 100 degrees c. or by long standing. with micrococcus aureus this action is often neutralized or overcome by a substance inhibitory to growth. the nature of these substances has not yet been determined, though several attempts along this line were made by ...191619867986
meningitis in an infant due to a hitherto undescribed organism, micrococcus florens.these virulence tests lead us to believe that micrococcus florens is pathogenic for mice, rabbits, and monkeys (macacus rhesus), producing septicemia, and that it will also cause purulent meningitis in the two latter animals. the combined results of the biological reactions and of the agglutination tests lead us to believe that we are dealing with an organism which, though morphologically similar to the meningococcus, yet is entirely distinct. a review of the literature fails to reveal any descr ...191719868183
a further experimental study on excitation of infections of the throat.improvements are described in the method of following temperature changes, and thus alterations in vasomotor tone, in exposed mucous membranes. invention of an applicator holder, by means of which more sure and stable apposition of the thermopile terminals to the mucous surface may be effected has been the chief advance. minor improvements have been the use of a saliva ejector and of better calibration technique. the palatine tonsils, like the palate, pharynx, and skin, react to chilling of the ...192019868432
the epidemiology of a rabbit respiratory infection : iii. nasal flora of laboratory rabbits.the bacteria constituting the nasal flora of rabbits at the rockefeller institute may be enumerated as follows in the order of their frequency: (1) micrococcus catarrhalis group, 80 per cent; (2) bacterium lepisepticum, 70 per cent; (3) gram-negative cocci which ferment dextrose, lactose, saccharose, maltose, and mannite, but not salicin and bacillus bronchisepticus, 40 per cent; (4) staphylococci, streptococci, and various intestinal bacilli which probably are localized at the external nares, 1 ...192419868889
the skin response of rabbits to non-hemolytic streptococci : i. description of a secondary reaction occurring locally after intradermal inoculation.1. rabbits inoculated intradermally with certain strains of green streptococci have well marked lesions which, after reaching a maximum size in 24 to 48 hours and then beginning to retrogress, show in over 50 per cent of the animals a secondary increase in size and other signs of inflammation about 8 or 9 days after inoculation. 2. this secondary reaction may follow the inoculation of a variety of strains of green-producing streptococci from various sources, of indifferent streptococci, and occa ...192619869172
streptococci in infectious (atrophic) arthritis and rheumatic fever.the question of the relationship of streptococci to the etiology of infectious arthritis and of rheumatic fever is of the utmost importance. if a streptococcus or group of streptococci could be shown to be associated see pdf for structure with either disease, some form of specific treatment might be available. the possibility of primary streptococcic infection as the cause of rheumatic fever, and, to a less extent, of acute infectious arthritis would seem to be a reasonable conjecture because of ...193019869811
a study on bacterial proteins with special consideration of gonococcus and meningococcus.methods of preparation and certain properties of the "nucleoproteins" of the following organisms are described: gonococcus, meningococcus, micrococcus catarrhalis, r pneumococcus, streptococcus hemolyticus, staphylococcus aureus. no essential differences between the nucleoproteins and the intact cells of gonococcus and meningococcus were observed in their ability to engender immune substances (precipitins), to induce bacterial allergy in rabbits, or to elicit cutaneous reactions (of the delayed ...193419870231
the carbohydrates of gonococcus and meningococcus : i. the alcohol-precipitable fraction.the alcohol-insoluble polysaccharides of gonococcus and meningococcus were found to contain 4.2 and 3.7 per cent nitrogen respectively, to be protein-free by chemical test, to reduce fehiing-benedict solution only after hydrolysis. they were non-toxic for rabbits and mice, and failed to engender antibodies (precipitins) in rabbits. they produced no cutaneous reactions in normal, snuffle-free rabbits, but caused typical allergic reactions of the delayed type in rabbits rendered hypersensitive to ...193419870232
the effect of lipids and serum albumin on bacterial growth.long chain fatty acids have been found to exhibit both inhibitory and stimulatory effects on the growth of tubercle bacilli and of a certain unidentified micrococcus culture. the toxicity of the fatty acids was much reduced or abolished by (a) esterification, even when the resulting product was a water-soluble ester, and (b) addition of crystalline serum albumin to the culture medium; other proteins tested were inactive in this respect. marked growth stimulation of the microorganisms studied was ...194719871602
bioactive constituents of the stem bark of mitrephora glabra.bioactivity-guided fractionation of the stem bark of mitrephora glabra yielded nine compounds, comprising three ent-kaurenoids (1-3), five polyacetylenic acids/esters (4-8), and one aporphine alkaloid, liriodenine (9). the structures of the six new compounds (1-3, 5, 7, and 8) were determined by spectroscopic data interpretation. all compounds were evaluated for their inhibitory activities against a panel of cancer cell lines and a battery of microorganisms.200919874044
the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment.in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ...201019874486
microbial diversity associated with algae, ascidians and sponges from the north coast of são paulo state, brazil.little is known about the microbial diversity associated with marine macroorganisms, despite the vital role microorganisms may play in marine ecosystems. the aim of the present study was to investigate the diversity of bacteria and fungi isolated from eight marine invertebrate and one algae samples. data derived from ardra and sequencing analyses allowed the identification of marine-derived microorganisms isolated from those samples. microbial strains identified up to the genus level revealed 14 ...201019879115
assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance.to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health.200919880150
bacterial resistance to antibiotics in acne vulgaris: an in vitro study.acne vulgaris is one of the most common skin disorders in youth especially during the puberty.200819882009
bloodstream infections due to micrococcus spp and intravenous epoprostenol. 200919888847
[bacterial biofilms on pvc tubing's inner surface of hemodialysis water treatment system].to determine the morphology, bacteria and endotoxin content of biofilms on the inner surface of pvc tubes in hemodialysis water treatment system.200919893257
new antibacterial and cytotoxic activities of falcarindiol isolated in crithmum maritimum l. leaf extract.bioassay-guided fractionation of a chloroformic extract obtained from crithmum maritimum leaves led to the chemical isolation of falcarindiol, a polyacetylene widely distributed within the apiaceae family. structure of this compound was confirmed by nmr and activity was tracked using a screening microplate bioassay. falcarindiol strongly inhibited the growth of micrococcus luteus and bacillus cereus, with a minimum inhibitory concentration (mic) value of 50 microg ml(-1). moreover, this compound ...201019922760
expression and purification of an antimicrobial peptide by fusion with elastin-like polypeptides in escherichia coli.different carrier molecules have been fused to antimicrobial polypeptides (amps) to facilitate recombinant protein expression and purification. some of them have improved the stability of amps and reduced the toxicity to host cells, but most current strategies still have some problems to be solved such as poor yield, low purity, high expense, time-consumption, and difficulty in scaling-up. here, we introduced the elastin-like polypeptides (elps) as a fusion partner to express an antimicrobial po ...201019924386
bacterial contamination of automotive fuels in a tropical region: the case of costa rica.microbial contamination of fuel has been the cause of several problems in transportation and storage of these products. due to the lack of previous studies related to these problems in costa rica, bacterial quality was evaluated biannually in automotive fuels stored in the four oil distribution facilities of the costa rican petroleum refinery (recope). in 12 oil storage tanks, for a total of 96 samples, mesophilic, heterotrophic aerobic/facultative counts (astm d6974-04) and identification of ba ...200919928449
characterization of expression, activity and role in antibacterial immunity of anopheles gambiae lysozyme c-1.there are eight lysozyme genes in the anopheles gambiae genome. transcripts of one of these genes, lysc-1, increased in anopheles gambiae cell line 4a3b by 24 h after exposure to heat-killed micrococcus luteus. lysozyme activity was also identified in conditioned media from the cell line from which the protein was purified to homogeneity using ion exchange and gel filtration. mass spectrometric analysis of the purified protein showed 100% identity to lysozyme c-1. purified lysozyme c-1 was teste ...201019932188
16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens.a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ...200919937215
an immune responsive multidomain galectin from bay scallop argopectens irradians.galectins are a family of beta-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. in the present study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from bay scallop argopectens irradians (designated aigal1) by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of aigal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. smart p ...201019944171
high prevalence of pseudomonas species in soil samples from ternate island-indonesia.in the present study, ten soil samples were examined and the ph of the soil was recorded. for bacterial isolation, a sterile nutrient and blood agars were used. gram stain and biochemical tests were done for identification. a total of 384 genus were isolated, 314 (81.8%) were identified as pseudomonas species of which 245 (78.0%) were pseudomonas aeruginosa, 42 (13.4%) were pseudomonas fluorescens, 13 (4.2%) were pseudomonas mallei, 10 (3.1%) were pseudomonas putida and 4 (1.3%) were pseudomonas ...200919947183
genome sequence of the fleming strain of micrococcus luteus, a simple free-living actinobacterium.micrococcus luteus (nctc2665, "fleming strain") has one of the smallest genomes of free-living actinobacteria sequenced to date, comprising a single circular chromosome of 2,501,097 bp (g+c content, 73%) predicted to encode 2,403 proteins. the genome shows extensive synteny with that of the closely related organism, kocuria rhizophila, from which it was taxonomically separated relatively recently. despite its small size, the genome harbors 73 insertion sequence (is) elements, almost all of which ...201019948807
decolorization of anthraquinone vat blue 4 by the free cells of an autochthonous bacterium, bacillus subtilis.uncontaminated soil, vat blue 4 contaminated soil and vat blue 4 effluent were screened for heterotrophic bacterial population and the bacterial density were found to be 19.3 x 10(4) colony forming units (cfu)/gm, 5.5 x 10(4) cfu/gm and 1.1 x 10(4) cfu/ml respectively. student's 't' test analysis affirmed that significant variation prevailed between the three set of 't' tests conducted (p<0.001 to 0.002). the heterotrophic bacterial population of dye contaminated soil comprised of 32.5% of pseud ...200919955647
antimicrobial effect of silver-impregnated cellulose: potential for antimicrobial therapy.silver has long been known to have antimicrobial activity. to incorporate this property into multiple applications, a silver-impregnated cellulose (sic) with low cytotoxicity to human cells was developed. sic differs from other silver treatment methods in that the leaching of silver particles is non-existent and the release of ionic silver is highly controlled.200919961601
differential transcriptomic responses of biomphalaria glabrata (gastropoda, mollusca) to bacteria and metazoan parasites, schistosoma mansoni and echinostoma paraensei (digenea, platyhelminthes).a 70-mer-oligonucleotide-based microarray (1152 features) that emphasizes stress and immune responses factors was constructed to study transcriptomic responses of the snail biomphalaria glabrata to different immune challenges. in addition to sequences with relevant putative id and gene ontology (go) annotation, the array features non-immune factors and unknown b. glabrata ests for functional gene discovery. the transcription profiles of b. glabrata (3 biological replicates, each a pool of 5 snai ...201019962194
functional analysis of a putative holin-like peptide-coding gene in the genome of bacillus licheniformis anba9.bhla, a putative holin-like protein of bacillus licheniformis anba9 expressed in escherichia coli bl21(de3) showed antibacterial activity against several gram-positive bacteria including methicillin-resistant staphylococcus aureus (mrsa) and micrococcus luteus. deletion analysis of bhla suggests that a hydrophobic transmembrane domain, bhlatm is essential for antibacterial activity. though the minimum inhibitory concentration (mic) of bhla was sevenfold lower than bhlatm, transmembrane domain de ...201019967339
decolorization and detoxification of sulfonated azo dye methyl orange by kocuria rosea mtcc 1532.kocuria rosea (mtcc 1532) showed 100% decolorization of methyl orange (50 mg l(-1)) under static condition. the optimum ph and temperature for dye decolorization was 6.8 and 30 degrees c, respectively. the k. rosea (mtcc 1532) showed maximum decolorization of methyl orange when growth medium containing yeast extract as compared to other substrates. the culture exhibited significant ability to decolorize repeated additions of dye, with reduction in time up to 12 h at eighth dye aliquot addition. ...201019969416
micrococcus pharyngis siccus endocarditis. 193219970024
streptococcus mucosus (howard) and its relation to micrococcus lanceolatus. 190219971463
a hitherto undescribed epizoötic among rabbits and rats, caused by a flagellate micrococcus. 190419971617
experimental work in relation to micrococcus rheumaticus and streptococcus pyogenes. 190619971705
a case of cerebellar abscess with isolation of micrococcus cereus albus. 191119972039
study i: studies on staphylococcus pyogenes aureus, albus and citreus, and on micrococcus tetragenus and m. catarrhalis. 191719972342
the fate of micrococcus aureus introduced into the blood stream of dogs. 191719972349
an unusual organism (micrococcus zymogenes) in a case of malignant endocarditis. 191219976274
purification and characterization of vanillin dehydrogenases from alkaliphile micrococcus sp. ta1 and neutrophile burkholderia cepacia tm1.vanillin dehydrogenases (vdhs) were purified and characterized from two bacterial strains that have different ph dependencies for growth. the alkaliphile micrococcus sp. ta1, isolated from an alkaline spa, can grow on several aromatic compounds such as ferulic acid, vanillin, vanillic acid, and protocatechuic acid under alkaline conditions. the neutrophile burkholderia cepacia tm1, which was isolated previously, also grew on the above-mentioned compounds because they functioned as the sole carbo ...201020002191
comparative analysis of micrococcus luteus isolates from blood cultures of patients with pulmonary hypertension receiving epoprostenol continuous infusion.during the period 2002-2008, at the national cardiovascular center, osaka, 28 micrococcus luteus isolates and one kocuria spp. isolate were obtained from blood cultures of pulmonary hypertension (ph) patients who were receiving continuous infusion therapy with epoprostenol. pulsed-field gel electrophoresis patterns of the isolates were unrelated, suggesting that the infections had multiple origins. the preparation of epoprostenol solution by patients themselves was thought to be a risk factor.200920012737
finding of the low molecular weight inhibitors of resuscitation promoting factor enzymatic and resuscitation activity.resuscitation promoting factors (rpf) are secreted proteins involved in reactivation of dormant actinobacteria, including mycobacterium tuberculosis. they have been considered as prospective targets for the development of new anti-tuberculosis drugs preventing reactivation of dormant tubercle bacilli, generally associated with latent tuberculosis. however, no inhibitors of rpf activity have been reported so far. the goal of this study was to find low molecular weight compounds inhibiting the enz ...200920016836
cem-101, a novel fluoroketolide: antimicrobial activity against a diverse collection of gram-positive and gram-negative bacteria.cem-101 is a novel fluoroketolide with reported high potency against diverse groups of gram-positive (micrococcus spp., viridans group streptococci, corynebacterium spp. listeria monocytogenes, clostridium spp., etc.) and gram-negative bacteria (neisseria gonorrhoeae, campylobacter jejuni, helicobacter pylori, bacteroides fragilis, shigella spp., etc.), including mycoplasma and ureaplasma, as well as bacteria commonly associated with community-acquired respiratory tract infections and skin and s ...201020022192
comparative immunological analysis of innate immunity activation after oral administration of wheat fermented extract to teleost fish.intradermal or oral administration of lipopolysaccharide derived from pantoea agglomerans (ip-pa1) has shown prophylactic and antitumor effects without serious side-effects. while it is known that tumor necrosis factor (tnf)-alpha produced by activated macrophages plays an important role in the expression mechanism following intradermal administration, details of the mechanism after oral administration remain unclear. in this study, the activation of innate immunity using fish as an animal model ...200920032450
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
genes involved in long-chain alkene biosynthesis in micrococcus luteus.aliphatic hydrocarbons are highly appealing targets for advanced cellulosic biofuels, as they are already predominant components of petroleum-based gasoline and diesel fuels. we have studied alkene biosynthesis in micrococcus luteus atcc 4698, a close relative of sarcina lutea (now kocuria rhizophila), which 4 decades ago was reported to biosynthesize iso- and anteiso-branched, long-chain alkenes. the underlying biochemistry and genetics of alkene biosynthesis were not elucidated in those studie ...201020038703
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