| comparison among four proposed direct blood culture microbial identification methods using maldi-tof ms. | matrix-assisted laser desorption-ionization time-of-flight (maldi-tof) mass spectrometry facilitates rapid and accurate identification of pathogens, which is critical for sepsis patients. in this study, we assessed the accuracy in identification of both gram-negative and gram-positive bacteria, except for streptococcus viridans, using four rapid blood culture methods with vitek maldi-tof-ms. we compared our proposed lysis centrifugation followed by washing and 30% acetic acid treatment method (m ... | 2016 | 27312817 |
| pharmacodynamics and differential activity of nitrofurantoin against esbl-positive pathogens involved in urinary tract infections. | although nitrofurantoin has been used for >60 years for the treatment of uncomplicated urinary tract infections, its pharmacodynamic properties are not fully explored. use is increasing because of increasing resistance to other antimicrobials due to esbls. | 2016 | 27278898 |
| antibacterial activities of the methanol extracts, fractions and compounds from harungana madagascariensis lam. ex poir. (hypericaceae). | harungana madagascariensis lam. ex poir. (hypericaceae) is used in folk medicine to treat a variety of human ailments, mainly antibacterial, antifungal, antiviral and viral infections. in the present study, the methanol extract from the leaves (hml) and bark (hmb) of this plant as well as fractions (hmba-c), sub-fractions (hmba1-5) and compounds isolated from hmba and hmbb namely betulinic acid (1), madagascin (2), ferruginin a (3) and kaempferol-3-o-β-d-glucopyranoside (4) were tested for their ... | 2016 | 27267830 |
| [incidence, predisposing risk factors for the development and spreading of acute intestinal infections in the north-eastern region of ukraine]. | using data of the branch statistical reporting of the state sanitary and epidemiological service in sumy region and sumy regional state laboratory of veterinary medicine, the incidence rate, modern risk factors for the development and spreading of acute infectious diarrheas were determined in the north-eastern region of ukraine. under the current conditions incidence rate indices of acute intestinal infections and food toxicoinfections are within the range of 159.8-193.6 per 100 thousands. pop. ... | 2016 | 27266031 |
| the bacterial ipra gene is conserved across enterobacteriaceae, is involved in oxidative stress resistance, and influences gene expression in salmonella enterica serovar typhimurium. | the ipra gene (formerly known as yaiv or stm0374) is located in a two-gene operon in the salmonella enterica serovar typhimurium genome and is associated with altered expression during spaceflight and rotating-wall-vessel culture conditions that increase virulence. however, ipra is uncharacterized in the literature. in this report, we present the first targeted characterization of this gene, which revealed that ipra is highly conserved across enterobacteriaceae we found that s typhimurium, esche ... | 2016 | 27246569 |
| extended spectrum beta-lactamase producing enterobacteriaceae causing bloodstream infections in rural ghana, 2007-2012. | high prevalence of extended spectrum beta-lactamase (esbl) producing enterobacteriaceae threatens treatment options for invasive bloodstream infections in sub-saharan africa. | 2016 | 27222489 |
| [distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps]. | to study the distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps. | 2016 | 27192905 |
| proteomic response of β-lactamases-producing enterobacter cloacae complex strain to cefotaxime-induced stress. | bacteria of the enterobacter cloacae complex are among the ten most common pathogens causing nosocomial infections in the usa. consequently, increased resistance to β-lactam antibiotics, particularly expanded-spectrum cephalosporins like cefotaxime (ctx), poses a serious threat. differential in-gel electrophoresis (dige), followed by lc-ms/ms analysis and bioinformatics tools, was employed to investigate the survival mechanisms of a multidrug-resistant e. hormaechei subsp. steigerwaltii 51 carry ... | 2016 | 27162211 |
| circulating of cmy-2 β-lactamase gene in weaned pigs and their environment in a commercial farm and the effect of feed supplementation with a clay mineral. | to investigate the mechanisms leading to an increase in the prevalence of blacmy -2 conferring resistance to ceftiofur in pigs receiving a feed medicated with chlortetracycline and penicillin, and to examine the effect of supplementation with a clay mineral on this phenomenon. | 2016 | 27138244 |
| a snapshot of co-resistance to carbapenems and tigecycline in clinical isolates of enterobacter cloacae. | enterobacter cloacae is one of the most common carbapenem-resistant enterobacteriaceae (cre) global wide. resistance to tigecycline, one of the few therapeutic options for cre infections, in carbapenem-resistant e. cloacae is of clinical significance. fourteen e. cloacae clinical isolates (ec1-ec14) co-resistant to tigecycline and carbapenems were studied. two tigecycline-susceptible/carbapenem-resistant isolates (ts1-ts2) were used for comparison. genotyping by pulsed-field gel electrophoresis ... | 2017 | 27136494 |
| structure and gene cluster of the o-antigen of enterobacter cloacae g3421. | the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3421 and studied by sugar analysis along with 1d and 2d (1)h and (13)c nmr spectroscopy. in addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. the following structure of the branched hexasaccharide repeating unit was established. the o-polysaccharide studied shares the β-l-rhap-(1→4)-α-l-rhap-(1→2)-α ... | 2016 | 27131290 |
| evidence from a new york city hospital of rising incidence of genetically diverse carbapenem-resistant enterobacter cloacae and dominance of st171, 2007-14. | | 2016 | 27118776 |
| survey of clinical extended-spectrum beta-lactamase-producing enterobacter cloacae isolates in a chinese tertiary hospital, 2012-2014. | a total of 72 consecutive and nonduplicate clinical extended-spectrum β-lactamase (esbl)-producing enterobacter cloacae isolates were collected from our hospital from 2012 to 2014 for analyzing the prevalence of plasmid-mediated quinolone resistance (pmqr) genes, 16s rrna methyltransferase (16s-rmtase) encoding genes, and carbapenem-hydrolyzing β-lactamase (chβl) genes, as well as integrons. pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) were carried out to charact ... | 2017 | 27116016 |
| phage-mediated shiga toxin (stx) horizontal gene transfer and expression in non-shiga toxigenic enterobacter and escherichia coli strains. | enterobacter cloacae m12x01451 strain recently identified from a clinical specimen produces a new stx1 subtype (stx1e) that was not neutralized by existing anti-stx1 monoclonal antibodies. acquisition of stx by ent. cloacae is rare and origin/stability of stx1e in m12x01451 is not known. in this study, we confirmed the ability of stx1a- and stx1e-converting phages from an escherichia coli o157:h7 strain rm8530 and m12x01451 respectively to infect several e. coli and ent. cloacae strains. stx1e w ... | 2016 | 27109772 |
| ceftazidime-avibactam activity against aerobic gram negative organisms isolated from intra-abdominal infections in united states hospitals, 2012-2014. | ceftazidime-avibactam is ceftazidime combined with the novel non-β-lactam β-lactamase inhibitor avibactam, which inhibits ambler class a (e.g., extended-spectrum β-lactamase [esbl] and kpc), class c, and some class d enzymes. we evaluated the activity of ceftazidime-avibactam against aerobic gram negative bacteria causing intra-abdominal infections (iai). | 2016 | 27104633 |
| what does the susceptible-dose dependent interpretive category for cefepime in esbl-producing bacteria? | in this study, we investigated the frequency of isolates included in the susceptible-dose dependent (sdd) category, according to the clinical and laboratory standards institute guidelines, carrying blatem, blashv and blactx genes among 92 klebsiella pneumoniae and 80 enterobacter cloacae clinical isolates. the presence of one or more extended-spectrum β-lactamases (esbls) genes was observed in 64% k. pneumoniae and 69% e. cloacae isolates. nineteen isolates were included in sdd interpretive cate ... | 2016 | 27077929 |
| [comparison of the modified hodge test and the carba np test for detection of carbapenemases in enterobacteriaceae isolates]. | a rapid, practical, and accurate identification of carbapenemase-producing enterobacteriaceae isolates is crucial for the implementation of appropriate infection control measures and proper treatment of the infections. for this purpose, a large number of phenotypic test methods have been developed, although none has 100% sensitivity and specificity. variations in sensitivity and specificity of these tests based on the type of beta-lactamase enzymes carried by that isolates might result in differ ... | 2016 | 27058324 |
| surveillance of wisconsin antibacterial susceptibility patterns. | antimicrobial resistance presents a threat to quality patient care. knowledge of localantibacterial susceptibility patterns can guide clinicians in empiric antibacterial administration andassist pharmacists and infectious disease physicians in development of appropriate therapeutic pathways. | 2016 | 27057577 |
| sonication of abdominal drains: clinical implications of quantitative cultures for the diagnosis of surgical site infection. | the use of drains in patients undergoing abdominal surgery has been a subject of debate for several decades. in this paper, the usefulness of quantitative cultures of sonicated abdominal drains for diagnosing surgical site of infection (ssi) and the association between culture results with patient outcome is evaluated. | 2016 | 27046739 |
| presence of antimicrobial resistance in coliform bacteria from hatching broiler eggs with emphasis on esbl/ampc-producing bacteria. | antimicrobial resistance is recognized as one of the most important global health challenges. broilers are an important reservoir of antimicrobial resistant bacteria in general and, more particularly, extended-spectrum β-lactamases (esbl)/ampc-producing enterobacteriaceae. since contamination of 1-day-old chicks is a potential risk factor for the introduction of antimicrobial resistant enterobacteriaceae in the broiler production chain, the presence of antimicrobial resistant coliform bacteria i ... | 2016 | 27011291 |
| carbapenemase-producing enterobacteriaceae: a 2-year surveillance in a hospital in iaşi, romania. | limited information is currently available about the prevalence of carbapenemase-producing enterobacteriaceae (cpe) in romania. | 2016 | 26974389 |
| optimizing prophylactic antibiotic practice for cardiothoracic surgery by pharmacists' effects. | pharmacists' role may be ideal for improving rationality of drug prescribing practice. we aimed to study the impact of multifaceted pharmacist interventions on antibiotic prophylaxis in patients undergoing clean or clean-contaminated operations in cardiothoracic department. a pre-test-post-test quasiexperimental study was conducted in a cardiothoracic ward at a tertiary teaching hospital in suzhou, china. patients admitted to the ward were collected as baseline group (2011.7-2012.12) and interve ... | 2016 | 26945362 |
| notes from the field: verona integron-encoded metallo-beta-lactamase-producing carbapenem-resistant enterobacteriaceae in a neonatal and adult intensive care unit--kentucky, 2015. | during august 4-september 1, 2015, eight cases of verona integron-encoded metallo-beta-lactamase (vim)-producing carbapenem-resistant enterobacteriaceae (cre) colonization were identified in six patients, using weekly active surveillance perirectal cultures in a kentucky tertiary care hospital. no cases of clinical infection or complications attributable to colonization were reported. four of the eight isolates were identified as enterobacter cloacae; other organisms included raoultella species ... | 2016 | 26914726 |
| enhanced efficacy of imipenem-colistin combination therapy against multiple-drug-resistant enterobacter cloacae: in vitro activity and a galleria mellonella model. | to investigate the in vitro and in vivo activity of imipenem-colistin combination against multidrug-resistant enterobacter cloacae infections in order to determine whether it should be explored further. | 2016 | 26906264 |
| control cross-sectional study evaluating an antibiotic prevention strategy in 30 pregnancies under clean intermittent self-catheterization and review of literature. | to assess the efficacy and safety of a weekly oral cycling antibiotic (woca) strategy to prevent uti in women. | 2016 | 26905030 |
| bacteriological quality of the wastewater used for irrigation at the vegetable farms in korle-bu teaching hospital, accra metropolis, ghana. | many developing countries, including ghana, are water stressed. as such, farmers, particularly those in urban areas, have adopted the use of wastewater for irrigation. this study evaluated the bacteriological water quality of the wastewater used for irrigation in the vegetable farms at korle-bu teaching hospital (kbth), accra metropolis, ghana. in all, 40 wastewater samples were collected and analysed bacteriologically using the total aerobic plate count method. the isolated bacteria were identi ... | 2017 | 26862131 |
| high prevalence of international esbl ctx-m-15-producing enterobacter cloacae st114 clone in animals. | the objective of this study was to characterize esbl-producing enterobacter cloacae isolated from animals and to compare their clonal distribution with that of human-related isolates. | 2016 | 26850718 |
| [contamination of workwear in medical doctors and nursing stuff]. | the hands of medical stuff are the most important vectors for the transmission of pathogens in the hospital. furthermore a "bare below the elbows dress code" has been introduced in great britain. | 2016 | 26837512 |
| [study on genotype and clinical characteristics of infection of carbapenemase-producing enterobacter cloacae]. | to study the genotypes and clinical characteristics of carbapenemase-producing enterobacter cloacae (e.cloacae), and lay the foundation for active control of nosocomial infection. | 2015 | 26815344 |
| characterisation of inca/c2 plasmids carrying an in416-like integron with the blavim-19 gene from klebsiella pneumoniae st383 of greek origin. | the complete nucleotide sequences of three multidrug resistance (mdr) inca/c-like plasmids from enterobacteriaceae isolates carrying the vim-type carbapenemase-encoding integrons in4863 (blavim-19-aaca7-dfra1-δaada1-smr2) or in4873 (blavim-1-aaca7-dfra1-δaada1-smr2) were determined, which are the first in416-like elements identified in greece. plasmids pkp-gr642 and pkp-gr8143 were from klebsiella pneumoniae st383 isolates, whereas plasmid pecl-gr4873 was from an enterobacter cloacae st88 isolat ... | 2016 | 26795022 |
| genetic background of novel sequence types of ctx-m-8- and ctx-m-15-producing escherichia coli and klebsiella pneumoniae from public wastewater treatment plants in são paulo, brazil. | the release of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae to the environment is a public health issue worldwide. the aim of this study was to investigate the genetic background of genes encoding esbls in wastewater treatment plants (wwtps) in são paulo, southeastern brazil. in 2009, during a local surveillance study, seven esbl-producing enterobacteriaceae strains were recovered from five wwtps and screened for esbl genes and mobile genetic elements. multilocus sequence ty ... | 2016 | 26782324 |
| molecular characteristics of extended spectrum β-lactamase and carbapenemase genes carried by carbapenem-resistant enterobacter cloacae in a chinese university hospital. | in recent years, the increase in the prevalence of carbapenem-resistant enterobacter cloacae (crec) has been significant and has become a global problem. the aim of this study is to characterize crec extended spectrum β-lactamases (esbls) and crec carbapenemase genes and evaluate the risk factors for crec infections in a university hospital in china. | 2015 | 26775390 |
| air-borne xylene degradation by bougainvillea buttiana and the role of epiphytic bacteria in the degradation. | the efficiency of xylene removal from contaminated air by thirteen perennial plants was studied. the results showed that bougainvillea buttiana had the highest xylene removal efficiency. different parts of b. buttiana such as stems, epicuticular waxes, and plant stomata (including microorganism-associated plant leaves) can uptake xylene 53.1±1.9%, 32.3±0.9, and 14.6±0.0%, respectively. metabolite products found in treated plants may result from stress or defense compounds triggered by exposure t ... | 2016 | 26773837 |
| medicinal plants used by a mbyá-guarani tribe against infections: activity on kpc-producing isolates and biofilm-forming bacteria. | the traditional use of medicinal plants for treatment of infectious diseases by an indigenous mbyá-guarani tribe from south brazil was assessed by evaluating the antibiotic and antibiofilm activities against relevant bacterial pathogens. aqueous extracts from 10 medicinal plants were prepared according to indigenous mbyá-guarani traditional uses. to evaluate antibiotic (od600) and antibiofilm (crystal violet method) activities, pseudomonas aeruginosa atcc 27853, staphylococcus epidermidis atcc 3 ... | 2015 | 26749812 |
| extended-spectrum antibodies protective against carbapenemase-producing enterobacteriaceae. | carbapenem-resistant enterobacteriaceae (cre) are responsible for worldwide outbreaks and antibiotic treatments are problematic. the polysaccharide poly-(β-1,6)-n-acetyl glucosamine (pnag) is a vaccine target detected on the surface of numerous pathogenic bacteria, including escherichia coli. genes encoding pnag biosynthetic proteins have been identified in two other main pathogenic enterobacteriaceae, enterobacter cloacae and klebsiella pneumoniae. we hypothesized that antibodies to pnag might ... | 2016 | 26747103 |
| phytases from enterobacter and serratia species with desirable characteristics for food and feed applications. | phytases are enzymes of great industrial importance with wide range of applications in animal and human nutrition. these catalyze the hydrolysis of phosphomonoester bonds in phytate, thereby releasing lower forms of myo-inositol phosphates and inorganic phosphate. addition of phytase to plant-based foods can improve its nutritional value and increase mineral bioavailability by decreasing nutritional effect of phytate. in the present investigation, 43 phytase positive bacteria on psm plates were ... | 2016 | 28330134 |
| bloodstream infections due to enterobacteriaceae among neonates in poland--molecular analysis of the isolates. | bloodstream infections (bsis) are associated with a significantly increased risk of fatality. no report has been found about the molecular epidemiology of enterobacteriaceae causing bsi in neonates in poland. the aim of this work was to determine the antibiotic resistance profiles, virulence gene prevalence, the epidemiological and genetic relationships among the isolates from enterobacteriaceae causing bsi in neonates with birth weight < 1501 g. antimicrobial susceptibility testing was performe ... | 2015 | 26638530 |
| recovery of bacteria and fungi from a leg wound. | acute and chronic wound infections can both be encountered in the deployed setting. these wounds are often contaminated by bacteria and fungi derived from the external environment. in this article, we present the case of a wound infection simultaneously colonized by enterobacter cloacae (a bacterial pathogen) and trichosporon asahii (an unusual fungal pathogen). we describe the examination and treatment of the patient and review the distinguishing characteristics of each organism. | 2015 | 26630106 |
| red wine polyphenols modulate fecal microbiota and reduce markers of the metabolic syndrome in obese patients. | this study evaluated the possible prebiotic effect of a moderate intake of red wine polyphenols on the modulation of the gut microbiota composition and the improvement in the risk factors for the metabolic syndrome in obese patients. ten metabolic syndrome patients and ten healthy subjects were included in a randomized, crossover, controlled intervention study. after a washout period, the subjects consumed red wine and de-alcoholized red wine over a 30 day period for each. the dominant bacterial ... | 2016 | 26599039 |
| characteristics of a novel aerobic denitrifying bacterium, enterobacter cloacae strain hnr. | a novel aerobic denitrifier strain hnr, isolated from activated sludge, was identified as enterobacter cloacae by16s rrna sequencing analysis. glucose was considered as the most favorable c-source for strain hnr. the logistic equation well described the bacterial growth, yielding a maximum growth rate (μmax) of 0.283 h(-1) with an initial no3 (-)-n concentration of 110 mg/l. almost all no3 (-)-n was removed aerobically within 30 h with an average removal rate of 4.58 mg n l(-1) h(-1). nitrogen b ... | 2016 | 26573667 |
| potential of phage cocktails in the inactivation of enterobacter cloacae--an in vitro study in a buffer solution and in urine samples. | the objective of this study was to compare the dynamics of three previously isolated phages for enterobacter cloacae in order to evaluate their ability to treat urinary tract infections (uti). the phages genomes, survival, host range, were characterized, and the host-phage dynamics was determined in culture medium and urine samples. the presence of prophages in bacteria, host recovery and development of resistance to phage after treatment was also evaluated. the growth of the e. cloacae was inhi ... | 2016 | 26541317 |
| synthesis and biological evaluation of curcuminoid derivatives. | many curcuminoid derivatives have been reported to have multiple biological activities. the aim of this study was to improve the biological activity of curcuminoids by synthesizing 16 new derivatives which combined cinnamic acids with curcuminoids, and we also analyzed the structure-activity relationship of the new compounds. almost all the new compounds showed encouraging activity, especially compound 7g. it had much better antioxidant activity than curcuminoids and vitamin c (vc), and also had ... | 2015 | 26521852 |
| outbreak of new delhi metallo-β-lactamase-1-producing enterobacter cloacae in an acute care hospital general ward in singapore. | autochthonous infections with new delhi metallo-β-lactamase-1 (ndm-1)-producing enterobacteriaceae have been reported in singapore since 2011, but occurrences of nosocomial transmission have not. we report an outbreak of ndm-1-producing enterobacter cloacae among adults admitted to an acute hospital's general ward. | 2016 | 26454748 |
| comparison of commonly used antimicrobial susceptibility testing methods for evaluating susceptibilities of clinical isolates of enterobacteriaceae and nonfermentative gram-negative bacilli to cefoperazone-sulbactam. | the aim of this study was to investigate the cefoperazone-sulbactam (cfp-sul) susceptibilities of important gram-negative bacteria (gnb) by agar dilution (reference method), disk diffusion, and two automated methods. | 2015 | 26454423 |
| epidemiology of carbapenem-resistant enterobacteriaceae in 7 us communities, 2012-2013. | carbapenem-resistant enterobacteriaceae (cre) are increasingly reported worldwide as a cause of infections with high-mortality rates. assessment of the us epidemiology of cre is needed to inform national prevention efforts. | 2015 | 26436831 |
| activity of temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam against carbapenem-non-susceptible enterobacteriaceae without carbapenemase production. | treatment options for multidrug-resistant gram-negative infections are scarce and therefore alternatives with a narrow spectrum or new agents are sought. antimicrobial susceptibility to temocillin, mecillinam, ceftazidime, and ceftazidime/avibactam was determined using etest and disk diffusion according to european committee on antimicrobial susceptibility testing (eucast) methodology. a total of 77 carbapenem-nonsusceptible enterobacteriaceae were studied, including klebsiella pneumoniae (26%), ... | 2015 | 26433746 |
| [time series analysis of enterobacter cloacae nosocomial infections in children hospitalized in the pediatric intensive care unit]. | to characterize the time distribution of the incidence of enterobacter cloacae nosocomial infections in children hospitalized in the pediatric intensive care unit (picu) of the first affiliated hospital of xinjiang medical university. | 2015 | 26412176 |
| [bacterial infections in the early period after allogeneic bone marrow transplantation]. | to study the incidence and risk factors of bacterial infections and the efficiency of empirical antibacterial therapy in patients in the early period after allogeneic hematopoietic stem cell transplantation (allo-hsct). | 2015 | 26390730 |
| purpura fulminans due to enterobacter cloacae. | | 2015 | 26370876 |
| construction of recombinant pichia pastoris carrying a constitutive avbd9 gene and analysis of its activity. | avian beta-defensin 9 (avbd9) is a small cationic peptide consisting of 41 amino acids that plays a crucial rule in innate immunity and acquired immunity in chickens. owing to its wide antibacterial spectrum, lack of a residue, and failure to induce bacterial drug resistance, avbd9 is expected to become a substitute for conventional antibiotics in the livestock and poultry industries. using the preferred codon of pichia pastoris, the mature avbd9 peptide was designed and synthesized, based on th ... | 2015 | 26370794 |
| regulation of indole-3-acetic acid biosynthesis by branched-chain amino acids in enterobacter cloacae uw5. | the soil bacterium enterobacter cloacae uw5 produces the rhizosphere signaling molecule indole-3-acetic acid (iaa) via the indolepyruvate pathway. expression of indolepyruvate decarboxylase, a key pathway enzyme encoded by ipdc, is upregulated by the transcription factor tyrr in response to aromatic amino acids. some members of the tyrr regulon may also be controlled by branched-chain amino acids and here we show that expression from the ipdc promoter and production of iaa are downregulated by v ... | 2015 | 26347301 |
| extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) infections: are carbapenem alternatives achievable in daily practice? | to avoid the use of carbapenems, alternatives such as cephamycin, piperacillin-tazobactam, and others are suggested for the treatment of extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-pe) infections. the aim of this study was to evaluate the frequency and the feasibility of antimicrobial de-escalation for esbl-pe-related infections. | 2015 | 26327124 |
| [analysis of clinical outcome and impact factors of twin-to-twin transfusion syndrome with anterior placenta treated by fetoscopic laser photocoagulation]. | to analyze the clinical outcome and impact factors of twin-to-twin transfusion syndrome (ttts) with anterior placenta treated by fetoscopic selective laser coagulation of placental vessels (slcpv). | 2015 | 26311450 |
| dissemination of multiresistant enterobacter cloacae isolates producing oxa-48 and ctx-m-15 in a spanish hospital. | twenty-one multiresistant enterobacter cloacae isolates producing oxa-48 (n=10), ctx-m-15 (n=7) or both (n=4) β-lactamases were detected in a spanish hospital during a 1-year period (june 2013 to june 2014). the isolates were also resistant to non-β-lactam antimicrobials, further complicating the therapeutic options. genotyping of the isolates identified two major clones (st74 and st66) that caused prolonged outbreaks in different buildings of the hospital as well as some sporadic isolates (st78 ... | 2015 | 26307466 |
| kpc and vim producing enterobacter cloacae strain from a hospital in northeastern venezuela. | an 83-year-old male patient is admitted to the central hospital in cumana, venezuela with severe urinary infection, history of hospitalizaions and prolonged antimicrobial treatments. a strain of enterobacter cloacae was isolated showing resistance to multiple types of antibiotics (only sensitive to gentamicin), with phenotype of serine- and metallo-carbapenemases. both, bla(vim-2) and bla(kpc) genes were detected in the isolate. this is the first report of an enterobacteriaceae species producing ... | 2015 | 26299058 |
| spontaneous bacterial and fungal infections in genetically engineered mice: is escherichia coli an emerging pathogen in laboratory mouse? | the impact of particular microbes on genetically engineered mice depends on the genotype and the environment. infections resulting in clinical disease have an obvious impact on animal welfare and experimentation. in this study, we investigated the bacterial and fungal aetiology of spontaneous clinical disease of infectious origin among the genetically engineered mice from our institution in relation to their genotype. a total of 63 mice belonging to 33 different mice strains, from severe immunod ... | 2015 | 26281439 |
| a new oxolane from enterobacter cloacae. | enterobacter cloacae is a highly pathogenic gram-negative proteobacterium which is responsible for a wide array of infections. in the present study, the fermentation culture of e. cloacae has yielded one new oxolane compound, rimboxo (1) in addition to three known compounds, i.e. maculosine (2), phenylacetic acid (3) and methyl myristate (4). these compounds were isolated and characterised using extensive chromatographic and spectroscopic methods, and were subjected to cytotoxicity evaluations. | 2016 | 26252083 |
| photophysical and antibacterial properties of complex systems based on smectite, a cationic surfactant and methylene blue. | solid or colloidal materials with embedded photosensitizers are promising agents from the medical or environmental perspective, where the direct use of photoactive solutions appears to be problematic. colloids based on layered silicates of the saponite (sap) and montmorillonite (mon) type, including those modified with dodecylammonium cations (c12) and photosensitizer--methylene blue (mb) were studied. two representatives of bacteria, namely enterobacter cloacae and escherichia coli, were select ... | 2015 | 26248233 |
| characterization of is26-composite transposons and multidrug resistance in conjugative plasmids from enterobacter cloacae. | shv-12 is the most widespread resistance determinant of enterobacter cloacae in taiwan; however, blashv-12 has rarely been mobilized. six multidrug-resistant e. cloacae isolates were collected. after conjugal transfer, plasmid profiling and analysis of incompatibility groups was performed to characterize the genetic context of blashv-12 -containing fragments. the presence of mobile genetic elements was demonstrated by pcr, cloning, sequencing and bioinformatics analyses. four different β-lactama ... | 2015 | 26223152 |
| [expression and characterization of a neutral enterobacter cloacae gx-3 invertase]. | to characterize a neutral invertase from enterobacter cloacae gx-3. | 2015 | 26211321 |
| two novel algicidal isolates kill chlorella pyrenoidosa by inhibiting their host antioxidase activities. | in the biocontrol of harmful algal blooms, there has been considerable interest about the role of algicidal bacteria in algicidal activity. in this experiment, two novel algicidal bacteria (strains np23 and am11) against chlorella pyrenoidosa were isolated from the baiguishan reservoir in china. phylogenetic analysis based on 16s rrna gene sequences revealed that strains np23 and am11 belonged to enterobacter cloacae and gibberella moniliformis, respectively. to further understand the algicidal ... | 2015 | 26194712 |
| identification and application of ssdna aptamers against h₃₇rv in the detection of mycobacterium tuberculosis. | microscopy of direct smear with the ziehl-neelsen stain is still broadly used in tuberculosis diagnosis. however, this method suffers from low specificity and is difficult to distinguish mycobacterium tuberculosis (mtb) from nontuberculosis mycobacterial (ntm), since all mycobacterial species are positive in ziehl-neelsen stain. in this study, we utilized whole cell selex to obtain species-specific aptamers for increasing the specificity of mtb detection. whole cell selex was performed in mtb re ... | 2015 | 26194558 |
| bioremediation of hexavalent chromium (vi) by a soil-borne bacterium, enterobacter cloacae b2-dha. | chromium and chromium containing compounds are discharged into the nature as waste from anthropogenic activities, such as industries, agriculture, forest farming, mining and metallurgy. continued disposal of these compounds to the environment leads to development of various lethal diseases in both humans and animals. in this paper, we report a soil borne bacterium, b2-dha that can be used as a vehicle to effectively remove chromium from the contaminated sources. b2-dha is resistant to chromium w ... | 2015 | 26191988 |
| monoterpenes as nitrofurantoin resistance modulating agents: minimal structural requirements, molecular dynamics simulations, and the effect of piperitone on the emergence of nitrofurantoin resistance in enterobacteriaceae. | the effects of different monoterpenes and 2-cyclohexen-1-one on the antibacterial activity of nitrofurantoin against resistant enterobacter cloacae, were compared and the minimal structural component of monoterpene required for the highest level of resistance-modulating activity was determined. subinhibitory concentrations of all compounds tested enhanced the antibacterial activity of nitrofurantoin against e. cloacae to different extents. the highest synergistic effect was observed for the mono ... | 2015 | 26174760 |
| tigecycline activity tested against carbapenem-resistant enterobacteriaceae from 18 european nations: results from the sentry surveillance program (2010-2013). | we evaluated the in vitro activity of tigecycline and selected comparator agents tested against carbapenem-resistant enterobacteriaceae (cre) isolated from european medical centers. a total of 14,286 clinically significant nonduplicate enterobacteriaceae isolates were collected from 18 european countries in 2010-2013. susceptibility testing was performed by clsi broth microdilution method, and isolates with a meropenem or imipenem mic at ≥4μg/ml were categorized as cre. selected cre strains were ... | 2015 | 26164275 |
| [outbreak of multidrug-resistant ctx-m-9-producing enterobacter cloacae complex in an intensive care unit]. | clinical and epidemiological description of an outbreak in an intensive care unit (icu) caused by a strain of multidrug-resistant enterobacter cloacae complex carrying a ctx-m-9-type extended-spectrum β-lactamase (esbl). | 2016 | 26139302 |
| dissemination of the multidrug-resistant extended-spectrum β-lactamase-producing escherichia coli o25b-st131 clone and the role of house crow (corvus splendens) foraging on hospital waste in bangladesh. | two hundred and thirty-eight faecal samples from crows foraging on hospital wastes were analysed for extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae. esbl-producing crow isolates were characterized and compared with 31 patient isolates. among the crows, 59% carried esbl producers. these included escherichia coli, klebsiella pneumoniae, raoultella terrigena and enterobacter cloacae harbouring the genes for ctx-m-1, ctx-m-15, ctx-m-55, ctx-m-79, and ctx-m-14. human isolates carri ... | 2015 | 26115863 |
| [extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in fecal samples at the national institute of child health, peru]. | to describe the frequency of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in fecal samples at the national institute of child health, lima, peru. | 2015 | 26102102 |
| coptisine attenuates obesity-related inflammation through lps/tlr-4-mediated signaling pathway in syrian golden hamsters. | it is known that obesity resulted from consumption of diets high in fat and calories and associated with a chronic low-grade inflammation. because the fat, sterol and bile acid metabolism of male syrian golden hamster are more similar to that of human, in the present study, high fat and high cholesterol (hfhc) induced obese hamsters were used to evaluate the anti-inflammation and hypolipidemic role of coptisine. the results showed that body weight, plasma lipid levels of total cholesterol (tc), ... | 2015 | 26073947 |
| assessment of the prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in ready-to-eat salads, fresh-cut fruit, and sprouts from the swiss market. | ready-to-eat (rte) prepacked salads and fruit have been successfully marketed for the last decade in switzerland and are increasingly important as a component of everyday diets. to determine whether extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae are present in rte salads, fresh-cut fruit, and sprouts on the swiss market, samples of 238 mixed and unmixed rte produce from a large production plant and 23 sprout samples from two sprout farms were analyzed. further, four samples fr ... | 2015 | 26038909 |
| a rapid and simple method to detect esbl in enterobacter cloacae based on mic of cefepime. | the aim of this study was to identify a rapid and simple phenotypic method for extended-spectrum β-lactamase (esbl) detection in enterobacter cloacae. | 2015 | 25992938 |
| arsenic rich himalayan hot spring metagenomics reveal genetically novel predator-prey genotypes. | bdellovibrio bacteriovorus are small deltaproteobacteria that invade, kill and assimilate their prey. metagenomic assembly analysis of the microbial mats of an arsenic rich, hot spring was performed to describe the genotypes of the predator bdellovibrio and the ecogenetically adapted taxa enterobacter. the microbial mats were enriched with bdellovibrio (1.3%) and several gram-negative bacteria including bordetella (16%), enterobacter (6.8%), burkholderia (4.8%), acinetobacter (2.3%) and yersinia ... | 2015 | 25953741 |
| molecular epidemiology of kpc-2-producing enterobacteriaceae (non-klebsiella pneumoniae) isolated from brazil. | in brazil, since 2009, there has been an ever increasing widespread of the bla(kpc-2) gene, mainly in klebsiella pneumoniae. this study aims to assess the molecular epidemiology and genetic background of this gene in enterobacteriaceae (non-k. pneumoniae) species from 9 brazilian states between 2009 and 2011. three hundred eighty-seven isolates were analyzed exhibiting nonsusceptibility to carbapenems, in which the bla(kpc-2) gene was detected in 21.4%. by disk diffusion and e-test, these isolat ... | 2015 | 25935630 |
| modified hodge test using mueller-hinton agar supplemented with cloxacillin improves screening for carbapenemase-producing clinical isolates of enterobacteriaceae. | increasing numbers of clinical isolates of enterobacteriaceae that produce carbapenemase are now being detected, with the most common carbapenemase found among enterobacteriaceae in japan being imp-1-type metallo-β-lactamase. clinical isolates of enterobacteriaceae harbouring carbapenemases may be resistant to carbapenem antimicrobial agents, despite apparent in vitro susceptibility when tested according to clinical and laboratory standards institute criteria. we evaluated the prevalence of carb ... | 2015 | 25934552 |
| [first report of carbapenemases in osijek-baranja county in imported enterobacter cloacae isolate in vitro susceptible to carbapenems]. | carbapenems are often the only therapeutic option to treat infections caused by multidrug-resistant gram-negative bacteria. emergence of carbapenemases in the isolates of enterobacteriaceae limits therapeutic options. carbapenem-resistant enterobacteriaceae have emerged since 2008 throughout croatia. in osijek-baranja county carbapenem-resistant strains of enterobacteriaceae were not reported until 2013. the first carbapenem-resistant strain (enterobacter cloacae) was identified in august 2013 i ... | 2015 | 25906543 |
| environmental monitoring of bacterial contamination and antibiotic resistance patterns of the fecal coliforms isolated from cauvery river, a major drinking water source in karnataka, india. | the present study focuses prudent elucidation of microbial pollution and antibiotic sensitivity profiling of the fecal coliforms isolated from river cauvery, a major drinking water source in karnataka, india. water samples were collected from ten hotspots during the year 2011-2012. the physiochemical characteristics and microbial count of water samples collected from most of the hotspots exhibited greater biological oxygen demand and bacterial count especially coliforms in comparison with contro ... | 2015 | 25896199 |
| [the biological characteristics of dominated agents of acute enteric infections]. | in 2003-2012 the rate of increase of morbidity of acute enteric infections caused by opportunistic microorganisms came to 3.6%. in the etiologic structure prevailed klebsiella pneumoniae, staphylococcus aureus and enterobacter cloacae. their percentage varied from 70.5% to 81.6%. the opportunistic microorganisms isolated from feces of patients with acute enteric infections and individuals from control group characterized by presence of pathogenic factors of broad spectrum. the adhesive character ... | 2014 | 25884080 |
| long-term arsenic monitoring with an enterobacter cloacae microbial fuel cell. | a microbial fuel cell was constructed with biofilms of enterobacter cloacae grown on the anode. bioelectrocatalysis was observed when the biofilm was grown in media containing sucrose as the carbon source and methylene blue as the mediator. the presence of arsenic caused a decrease in bioelectrocatalytic current. biofilm growth in the presence of arsenic resulted in lower power outputs whereas addition of arsenic showed no immediate result in power output due to the short term arsenic resistance ... | 2015 | 25862430 |
| isolation and characterization of endophytic plant growth-promoting bacteria from date palm tree (phoenix dactylifera l.) and their potential role in salinity tolerance. | endophytic bacteria were isolated from date palm (phoenix dactylifera l.) seedling roots, characterized and tested for their ability to help plants grow under saline conditions. molecular characterization showed that the majority of these strains belonged to the genera bacillus and enterobacter and had different degrees of resistance to various antibiotics. some of these strains were able to produce the enzyme 1-aminocyclopropane-1-carboxylic acid (acc) deaminase and the plant growth regulatory ... | 2015 | 25860542 |
| bioaugmentation of biogas production by a hydrogen-producing bacterium. | the rate-limiting nature of the hydrogen concentration prevailing in the anaerobic digester has been recognized, but the associated alterations in the microbial community are unknown. in response to the addition of enterobacter cloacae cells in laboratory anaerobic digesters, the level of biogas production was augmented. terminal restriction fragment length polymorphism (t-rflp) and real-time polymerase chain reaction (real-time pcr) were used to study the survival of mesophilic hydrogen-produci ... | 2015 | 25836037 |
| the three-dimensional structure of vim-31--a metallo-β-lactamase from enterobacter cloacae in its native and oxidized form. | the metallo-β-lactamase vim-31 differs from vim-2 by only two tyr224his and his252arg substitutions. located close to the active site, the tyr224his substitution is also present in vim-1, vim-4, vim-7 and vim-12. the vim-31 variant was reported in 2012 from enterobacter cloacae and kinetically characterized. it exhibits globally lower catalytic efficiencies than vim-2. in the present study, we report the three-dimensional structures of vim-31 in its native (reduced) and oxidized forms. the so-ca ... | 2015 | 25825035 |
| detection and characterization of extended-spectrum beta-lactamase-producing enterobacteriaceae in high-risk patients in an irish tertiary care hospital. | extended-spectrum beta-lactamase-producing enterobacteriaceae (esbl-e) are gram-negative, multi-drug-resistant organisms that are of major clinical significance among immunocompromised patients in high-risk areas in hospital settings. in ireland, the number of esbl-e bloodstream infections is increasing. | 2015 | 25799484 |
| the extract of hypericum ascyron l. induces bacterial cell death through apoptosis pathway. | hypericum ascyron l. (h. ascyron l.) has been used as a traditional medicine for the treatment of wounds, swelling, headache, nausea, stomach ache, abscesses, dysentery and chronic bronchitis. pharmacological studies are necessary to provide a scientific basis to substantiate their traditional use. in this study, antimicrobial effect and its mechanism of the h. ascyron l. extract was investigated. | 2015 | 25796407 |
| antibacterial activity of fosfomycin against uropathogens. | in recent years, the rising rates of resistance to antimicrobial drugs among pathogens have caused great difficulty for clinicians treating infectious diseases. the aim of the study was to assess the curative effect of fosfomycin in treating urinary tract infections (utis) in china. | 2014 | 25792115 |
| pathogenicity of bacteria isolated from gut of spodoptera litura (lepidoptera: noctuidae) and fitness costs of insect associated with consumption of bacteria. | gut microbes contribute to the health of insects and perturbations in the composition or location of gut microbiota can lead to pathological states and host mortality. we explored the culturable bacterial community in the gut of spodoptera litura (fab.) larvae, which is a polyphagous pest. bacterial isolates were identified as microbacterium arborescens (sl6), enterococcus casseliflavus (sl10) and enterobacter cloacae (sl11) by using culture dependent technique based on 16s rrna gene sequencing. ... | 2015 | 25725116 |
| whole genome sequence of enterobacter ludwigii type strain en-119t, isolated from clinical specimens. | enterobacter ludwigii strain en-119(t) is the type strain of e. ludwigii, which belongs to the e. cloacae complex (ecc). this strain was first reported and nominated in 2005 and later been found in many hospitals. in this paper, the whole genome sequencing of this strain was carried out. the total genome size of en-119(t) is 4952,770 bp with 4578 coding sequences, 88 trnas and 10 rrnas. the genome sequence of en-119(t) is the first whole genome sequence of e. ludwigii, which will further our und ... | 2015 | 25724532 |
| structure and genetics of the o-antigen of enterobacter cloacae g3054 containing di-n-acetylpseudaminic acid. | mild acid degradation of the lipopolysaccharide of enterobacter cloacae g3054 resulted in the cleavage of the o-polysaccharide at the linkage of residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid (di-n-acetylpseudaminic acid, pse5ac7ac) in the main chain. the resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)h and (13)c nmr spectroscopy, and the following structure of the branched pentasaccharide o-un ... | 2015 | 25723621 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts. | in immunosuppressed hosts, rapid identification of microorganisms of bloodstream infections is crucial to ensuring effective antimicrobial therapy. conventional culture requires up to 72 h from sample collection to pathogen identification. | 2015 | 25704776 |
| bacteriological investigation of chronic wounds in a specialized wound healing department: a retrospective analysis of 107 cases. | to investigate the information of chronic wounds, especially in the aspect of microbiological profile and to explore the relationship between the wound culture result and chronic wounds infection, we retrospectively reviewed the medical records of 107 patients with chronic wounds from january 2011 to december 2013. the sociodemographic data, wound-related information, therapeutic type, and wound infection status were extracted. microbial specimens were obtained and processed using standard hospi ... | 2015 | 25700708 |
| assessment of species and antimicrobial resistance among enterobacteriaceae isolated from mallard duck faeces. | mallard ducks have demonstrated to be a likely reservoir for zoonotic e. coli strains; thus, it is possible that these ducks could also act as a reservoir for other enterobacteriaceae members. the present study was initiated to evaluate the species distribution of enterobacteriaceae other than e. coli in 175 fresh faecal samples collected from a population of mallard ducks. sixty-four samples displayed detectable colonies of enterobacteriaceae (excluding e. coli), which resulted in 75 pulsed-fie ... | 2015 | 25697309 |
| enterobacter cloacae pericardial effusion in a frail elderly patient. | we report a case of a frail 82-year-old man with seronegative rheumatoid arthritis and a recent pacemaker insertion, admitted with pulmonary oedema and a symptomatic pericardial effusion. he was treated with diuretics and an urgent pericardiocentesis, a sample from which cultured enterobacter cloacae. a subsequent abdominal ct scan revealed faecal loading, an abnormal anorectal canal and sigmoid colon and a bowel perforation. endoscopy, biopsies and histopathology confirmed a diagnosis of cytome ... | 2015 | 25697299 |
| galleria mellonella as an in vivo model for assessing the efficacy of antimicrobial agents against enterobacter cloacae infection. | enterobacter cloacae is a well-recognized nosocomial pathogen. use of a rapid, in vivo infection model for e. cloacae that can determine the efficacy of antibiotic therapies could help facilitate screening for new treatments. nonmammalian model systems of infection, such as galleria mellonella, have significant logistical and ethical advantages over mammalian models. | 2017 | 25682237 |
| clonal distribution of multidrug-resistant enterobacter cloacae. | a multilocus sequence typing (mlst) scheme including 7 housekeeping genes was used to evaluate whether the current spread of multidrug-resistant enterobacter cloacae isolates worldwide might be associated to specific successful clones. fifty e. cloacae clinical isolates of worldwide origin, with various β-lactamase content, and recovered at different periods of time were studied. forty-four sequence types were identified, highlighting a high clonal diversity with 3 main lineages. this study reve ... | 2015 | 25680336 |
| [detection of serinocarbapenemases class a and other mechanisms of enzymatic resistance to β-lactams in enterobacteria strains with diminished susceptibility to carbapenems isolated of patients in a university hospital]. | the emergence of carbapenemase mediated resistance in enterobacteriaceae has a strong clinical impact. this study aimed to do a genotypic and phenotypic characterization of the enzymatic resistance to β-lactams in clinical enterobacteriaceae isolates with decreased susceptibility to carbapenems in a university medical center in santiago. | 2014 | 25679924 |
| pancreatic fistulae secondary to trypsinogen activation by pseudomonas aeruginosa infection after pancreatoduodenectomy. | pancreatic fistula after pancreatoduodenectomy (pd) is associated with high mortality and morbidity. trypsinogen activation and bacteria, although hypothesized to be interrelated etiopathogenetically, have not had their relationship and pathogenic mechanisms elucidated. this study investigated bacterial involvement in pancreatic juice activation perioperatively after pd at sites of pancreatic fistula formation. | 2015 | 25678202 |
| studies of house dust mites can now fully embrace the "-omics" era. | | 2015 | 25662308 |
| enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains. | in this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80°c, 30 min) sludge and liquid (lb-grown) cultures of escherichia coli xl1-blue/enterobacter cloacae dsm 16657 strains for bioaugmentation purposes. the results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in t ... | 2015 | 25661265 |
| the dissemination mode of drug-resistant genes in enterobacter cloacae. | enterobacter cloacae (e. cloacae) infection has the highest mortality rate among enterobacter infections. this study aimed to determine the prevalence and the transmission route of the class i integron, qnr genes, and ctx-m esbls genes in clinical isolates and to analyse the association between the prevalence of mdr genes and the antibiotic resistance of e. cloacae. | 2015 | 25657163 |
| acquisition of carbapenem-resistant gram-negative bacilli in intensive care unit: predictors and molecular epidemiology. | we had for aim to determine the risk factors for acquiring carbapenem-intermediate or -resistant gram-negative bacilli (cr-gnb) in an intensive care unit (icu) and to identify the resistance mechanisms involved. | 2015 | 25640914 |
| high prevalence of ctx-m-15 and first report of ctx-m-3, ctx-m-22, ctx-m-28 and plasmid-mediated ampc beta-lactamase producing enterobacteriaceae causing urinary tract infections in bosnia and herzegovina in hospital and community settings. | to investigate molecular epidemiology of extended-spectrum β-lactamase/esbl and plasmid-mediated ampc β-lactamase/pampc producing gram-negative bacteria causing urinary tract infections (utis) in zenica-doboj canton, bosnia and herzegovina, in the period decembar 2009-may 2010. | 2015 | 25638292 |