| a multicomponent sugar phosphate sensor system specifically induced in bacillus cereus during infection of the insect gut. | using a previously developed bacillus cereus in vivo expression technology (ivet) promoter trap system, we showed that spsa, a gene of unknown function, was specifically expressed in the larval gut during infection. search for gut-related compounds inducing spsa transcription identified glucose-6-phosphate (g6p) as an activation signal. analysis of the spsa-related 5-gene cluster indicated that spsa is part of a new sugar phosphate sensor system composed of a 2-component system (tcs) encoded by ... | 2012 | 22611084 |
| synthesis and antibacterial activities of 1-alkyl-3-methacryloyl (acryloyl) of benzimidazolone (thione) derivatives. | a series of (28) 1-alkyl-3-methacryloyl (acryloyl)-benzimidazolone (thione) deriv-atives were synthesized. the structures of the new derivatives were confirmed by (1)h-nmr, (13)c-nmr and esi-ms spectral analysis. the antibacterial activities of these compounds against several strains of bacteria, such as bacillus cereus, bacillus subtilis, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, were evaluated by methods of paper disc-diffusion and broth mciro-dilution. methacryloyl d ... | 2012 | 22606011 |
| possible contamination of organ preservation fluid with bacillus cereus: the united kingdom response. | we describe here the united kingdom (uk) response following the recent international recall of an organ preservation fluid owing to potential bacillus cereus contamination. this fluid is used for the transport of solid organs and pancreatic islet cells for transplant. we detail the response mechanisms, including the initial risk stratification, investigatory approaches, isolate analysis and communications to professional bodies. this report further lays out the potential need for enhanced survei ... | 2012 | 22587955 |
| rapid detection of bacterial contamination of platelet-rich plasma-derived platelet concentrates using flow cytometry. | flow cytometry can be used to detect bacterial contamination of platelet products. in this study, we investigated whether the incubation of a minimal volume of platelet-rich plasma (prp)-derived platelet concentrates (pcs) with growth medium improved the analytical sensitivity of flow cytometry. five bacterial strains (staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, klebsiella pneumoniae, and escherichia coli) were used. platelets were inoculated with 10, 10(2), and 10(3) cfu ... | 2012 | 22585614 |
| investigations of antimicrobial activity of some cameroonian medicinal plant extracts against bacteria and yeast with gastrointestinal relevance. | resistance against antibiotics of many bacteria is accumulating. therefore, searches for new substances with antimicrobial activity have become an urgent necessity. medicinal plants are frequently used in popular medicine as remedies for many infectious diseases (intestinal infection, malaria, tuberculosis, etc.). | 2012 | 22583961 |
| membrane interaction and antibacterial properties of chensinin-1, an antimicrobial peptide with atypical structural features from the skin of rana chensinensis. | many antimicrobial peptides from amphibian skin have been purified and structurally characterized and may be developed as therapeutic agents. here we describe the antibacterial properties and membrane interaction of chensinin-1, a cationic arginine/histidine-rich antimicrobial peptide, from the skin secretions of rana chensinensis. the amino acid composition, sequence, and atypical structure of chensinin-1 differ from other known antimicrobial peptides from amphibian skin. chensinin-1 exhibited ... | 2012 | 22581068 |
| dimethyl disulfide is an induced systemic resistance elicitor produced by bacillus cereus c1l. | bacillus cereus c1l is a plant growth-promoting rhizobacterium and can elicit induced systemic resistance (isr) in plants against necrotrophic pathogens. however, little is known about isr elicitors produced by b. cereus c1l, and no isr elicitor has been identified and characterised. therefore, the objective of this study is to identify volatile isr elicitor(s) produced by b. cereus c1l. | 2012 | 22573612 |
| cody orchestrates the expression of virulence determinants in emetic bacillus cereus by impacting key regulatory circuits. | bacillus cereus causes gastrointestinal diseases and local and systemic infections elicited by the depsipeptide cereulide, enterotoxins, phospholipases, cytolysins and proteases. the plcr-papr quorum sensing system activates the expression of several virulence factors, whereas the spo0a-abrb regulatory circuit partially controls the plasmid-borne cereulide synthetase (ces) operon. here, we show that cody, a nutrient-responsive regulator of gram-positive bacteria, has a profound effect on both re ... | 2012 | 22571587 |
| characterization of boron resistant and accumulating bacteria lysinibacillus fusiformis m1, bacillus cereus m2, bacillus cereus m3, bacillus pumilus m4 isolated from former mining site, hokkaido, japan. | boron is known to be widespread environmental contaminant that is relatively mobile in soil when compared to other metal contaminants. the present study made an attempt to isolate and characterize the boron resistant and accumulating bacteria from former mining site at hokkaido, japan. four potential strains m1, m2, m3 and m4 were selected based on high degree of boron and heavy metal resistances. the morphological, biochemical and 16s rdna sequencing analysis of mining bacteria revealed that th ... | 2012 | 22571522 |
| complete genome sequence of bacillus cereus bacteriophage pbc1. | bacillus cereus is a ubiquitous, spore-forming bacterium associated with food poisoning cases. to develop an efficient biocontrol agent against b. cereus, we isolated lytic phage pbc1 and sequenced its genome. pbc1 showed a very low degree of homology to previously reported phages, implying that it is novel. here we report the complete genome sequence of pbc1 and describe major findings from our analysis. | 2012 | 22570248 |
| sequence analysis of inducible prophage phis3501 integrated into the haemolysin ii gene of bacillus thuringiensis var israelensis atcc35646. | diarrheic food poisoning by bacteria of the bacillus cereus group is mostly due to several toxins encoded in the genomes. one of them, cytotoxin k, was recently identified as responsible for severe necrotic syndromes. cytotoxin k is similar to a class of proteins encoded by genes usually annotated as haemolysin ii (hlyii) in the majority of genomes of the b. cereus group. the partially sequenced genome of bacillus thuringiensis var israelensis atcc35646 contains several potentially induced proph ... | 2012 | 22567391 |
| role of side-edge site of sphingomyelinase from bacillus cereus. | bacillus cereus sphingomyelinase (bc-smase) belongs to the mg(2+)-dependent neutral sphingomyelinase (nsmase) which hydrolyzes sphingomyelin (sm) to produce phosphocholine and ceramide, and acts as an extracellular hemolysin. bc-smase has two metal ion-binding sites in a long horizontal cleft across the molecule, with one mg(2+) in the central region of the cleft and one divalent metal ion at the side-edge of the cleft. the role of the mg(2+) at the side-edge of the long horizontal cleft in bc-s ... | 2012 | 22564743 |
| antimicrobial activity of platelet (plt)-poor plasma, plt-rich plasma, plt gel, and solvent/detergent-treated plt lysate biomaterials against wound bacteria. | platelet (plt) gels exhibit antimicrobial activity useful for wound healing. the nature of the antibacterial component(s) is unknown. | 2013 | 22563709 |
| solid-state fermentation: tool for bioremediation of adsorbed textile dyestuff on distillery industry waste-yeast biomass using isolated bacillus cereus strain ebt1. | bioremediation of textile dyestuffs under solid-state fermentation (ssf) using industrial wastes as substrate pose an economically feasible, promising, and eco-friendly alternative. the purpose of this study was to adsorb red m5b dye, a sample of dyes mixture and a real textile effluent on distillery industry waste-yeast biomass (diw-yb) and its further bioremediation using bacillus cereus ebt1 under ssf. textile dyestuffs were allowed to adsorb on diw-yb. diw-yb adsorbed dyestuffs were decolori ... | 2013 | 22562346 |
| genome organization of temperate phage 11143 from emetic bacillus cereus nctc11143. | a temperate phage was isolated from emetic bacillus cereus nctc 11143 by mitomycin c and characterized by transmission electron microscopy and dna and protein analyses. whole genome sequencing of bacillus phage 11143 was performed by gs-flx. the phage has a dsdna genome of 39,077 bp and a 35% g+c content. bioinformatic analysis of the phage genome revealed 49 putative orfs involved in replication, morphogenesis, dna packaging, lysogeny, and host lysis. bacillus phage 11143 could be classified as ... | 2012 | 22561859 |
| a urea channel from bacillus cereus reveals a novel hexameric structure. | urea is exploited as a nitrogen source by bacteria, and its breakdown products, ammonia and bicarbonate, are employed to counteract stomach acidity in pathogens such as helicobacter pylori. uptake in the latter is mediated by urei, a uac (urea amide channel) family member. in the present paper, we describe the structure and function of uacbc, a homologue from bacillus cereus. the purified channel was found to be permeable not only to urea, but also to other small amides. cd and ir spectroscopy r ... | 2012 | 22553922 |
| antibacterial anthraquinone derivatives from a sea anemone-derived fungus nigrospora sp. | chemical investigation of a marine-derived fungus nigrospora sp., isolated from an unidentified sea anemone, yielded two new hydroanthraquinone analogues, 4a-epi-9α-methoxydihydrodeoxybostrycin (1) and 10-deoxybostrycin (2), together with seven known anthraquinone derivatives (3-9). the structures of the two new compounds were established through extensive nmr spectroscopy as well as a single-crystal x-ray diffraction analysis using cu kα radiation. the antibacterial activities of compounds 1-9 ... | 2012 | 22545792 |
| two piperazic acid-containing cyclic hexapeptides from streptomyces alboflavus 313. | two novel cyclic hexapeptides, designated nw-g10 (1) and nw-g11 (2), were isolated from the fermentation broth of streptomyces alboflavus 313. their relative structures were elucidated on the basis of extensive spectroscopic analysis, and the absolute configurations of several constituent amino acids were determined by marfey's method. nw-g10 (1) and nw-g11 (2) exhibited significant activity against gram-positive bacteria, such as bacillus cereus, bacillus subtilis and staphylococcus aureus, inc ... | 2012 | 22543750 |
| cody, a pleiotropic regulator, influences multicellular behaviour and efficient production of virulence factors in bacillus cereus. | in response to nutrient limitation in the environment, the global transcriptional regulator cody modulates various pathways in low g+c gram-positive bacteria. in bacillus subtilis cody triggers adaptation to starvation by secretion of proteases coupled to the expression of amino acid transporters. furthermore, it is involved in modulating survival strategies like sporulation, motility, biofilm formation, and cody is also known to affect virulence factor production in pathogenic bacteria. in this ... | 2012 | 22540344 |
| global mrna decay analysis at single nucleotide resolution reveals segmental and positional degradation patterns in a gram-positive bacterium. | recent years have shown a marked increase in the use of next-generation sequencing technologies for quantification of gene expression (rna sequencing, rna-seq). the expression level of a gene is a function of both its rate of transcription and rna decay, and the influence of mrna decay rates on gene expression in genome-wide studies of gram-positive bacteria is under-investigated. | 2012 | 22537947 |
| on the active site of mononuclear b1 metallo β-lactamases: a computational study. | metallo-β-lactamases (mβls) are zn(ii)-based bacterial enzymes that hydrolyze β-lactam antibiotics, hampering their beneficial effects. in the most relevant subclass (b1), x-ray crystallography studies on the enzyme from bacillus cereus point to either two zinc ions in two metal sites (the so-called '3h' and 'dch' sites) or a single zn(ii) ion in the 3h site, where the ion is coordinated by asp120, cys221 and his263 residues. however, spectroscopic studies on the b1 enzyme from b. cereus in the ... | 2012 | 22532071 |
| biochemical analysis and investigation on the prospective applications of alkaline protease from a bacillus cereus strain. | proteases have prospective financial and environment-friendly applications; hence attention is focused currently on the finding of new protease producing microorganism so as to meet the requirements of industry. a thermophilic bacterial strain producing extracellular protease activity was isolated from soil and identified as bacillus cereus by analysis of 16s rrna. protease production by the microorganism was improved by studying the impact of the type of nitrogen and carbon source, fermentation ... | 2012 | 22528469 |
| characterization of an endolysin, lysbps13, from a bacillus cereus bacteriophage. | use of bacteriophages as biocontrol agents is a promising tool for controlling pathogenic bacteria including antibiotic-resistant bacteria. not only bacteriophages but also endolysins, the peptidoglycan hydrolyzing enzymes encoded by bacteriophages, have high potential for applications as biocontrol agents against food-borne pathogens. in this study, a putative endolysin gene was identified in the genome of the bacteriophage bps13, which infects bacillus cereus. in silico analysis of this endoly ... | 2012 | 22524239 |
| iron regulates expression of bacillus cereus hemolysin ii via global regulator fur. | the capacity of pathogens to respond to environmental signals, such as iron concentration, is key to bacterial survival and establishment of a successful infection. bacillus cereus is a widely distributed bacterium with distinct pathogenic properties. hemolysin ii (hlyii) is one of its pore-forming cytotoxins and has been shown to be involved in bacterial pathogenicity in a number of cell and animal models. unlike many other b. cereus pathogenicity factors, hlyii is not regulated by pleiotropic ... | 2012 | 22522892 |
| development of sandwich enzyme-linked immunosorbent assay for the detection of cronobacter muytjensii (formerly called enterobacter sakazakii). | this study aimed to produce a polyclonal antibody against cronobacter muytjensii (c. muytjensii, formerly called enterobacter sakazakii) and to develop an immunoassay for its detection. the optimum production of rabbit anti-c. muytjensii immunoglobulin g (igg) and chicken anti-c. muytjensii igy was reached in weeks 8 and 9, respectively. purification of rabbit anti-c. muytjensii igg from immunized rabbit sera was accomplished using the caprylic acid and ammonium sulfate precipitation method. as ... | 2012 | 22519814 |
| partitioning studies of l-glutaminase production by bacillus cereus mtcc 1305 in different peg-salt/dextran. | partitioning studies of l-glutaminase production by bacillus cereus mtcc 1305 was carried out in different peg-salt/peg-dextran system. the partitioning value of l-glutaminase increased with increasing molecular weight of peg from 2000-4000 kda and decreased with higher molecular weight of 6000 kda. phase system of peg 4000 (8.5%)/dextran t500 (9.5%) was selected for the extractive fermentative production of l-glutaminase on the basis maximum partition coefficient (1.31). the production of l-glu ... | 2012 | 22513254 |
| foodborne outbreaks surveillance in the piedmont region, italy (2002-2009). | this article summarises the results of eight years (2002-2009) of investigations on food borne disease outbreaks carried out the regional agency for public health in piedmont, which is an italian region (4,432,571 citizens, 2008). | 2012 | 22508645 |
| inactivation kinetics of spores of bacillus cereus strains treated by a peracetic acid-based disinfectant at different concentrations and temperatures. | the purpose of this study was to assess the effect of a commercial peracetic acid-based disinfectant against spores of bacillus cereus, to identify the most influential factor for the final number of microorganisms after different disinfection procedures, and to evaluate the nature of the inactivation kinetics. the spores of four different strains of b. cereus (dsm 318, 4312, 4313, and 4384) were treated with five different disinfectant concentrations (0.25%, 0.5%, 1.0%, 1.5%, and 2.0% [w/v]) at ... | 2012 | 22506696 |
| microbiological and physicochemical characterisation of caxiri, an alcoholic beverage produced by the indigenous juruna people of brazil. | caxiri is a traditional fermented alcoholic beverage produced from cassava and sweet potatoes by the indigenous juruna or yudjá people in brazil. our results showed that caxiri fermentation is invariably associated with the following: (i) an increase in the total microbial population, with yeast being the largest group detected; (ii) a decrease in reducing sugars, malic, tartaric, succinic, oxalic and propionic acid; and (iii) a final product characterised by a high content of ethanol and a high ... | 2012 | 22497838 |
| [screening and identification of a microcystin-degrading bacterium strain and its enzymatic degradation of microcystin-lr by intracellular extract of bacillus cereus]. | to provide effective microorganisms for the treatment of water polluted by microcystins (mcs), the strains capable of biodegrading microcystin lr (mc-lr) were isolated from the sediment of chaohu lake. the degradation of microcystin-lr by the intracellular extracts of the strains were studied. | 2012 | 22489466 |
| microbiological performance of a food safety management system in a food service operation. | the microbiological performance of a food safety management system in a food service operation was measured using a microbiological assessment scheme as a vertical sampling plan throughout the production process, from raw materials to final product. the assessment scheme can give insight into the microbiological contamination and the variability of a production process and pinpoint bottlenecks in the food safety management system. three production processes were evaluated: a high-risk sandwich p ... | 2012 | 22488059 |
| antimicrobial effects of silver nanoparticles against bacterial cells adhered to stainless steel surfaces. | given the increasing number of antibiotic-resistant bacteria and the need to synthesize new antimicrobials, silver has attracted interest in the scientific community because of its recognized antimicrobial activity. this study aimed to evaluate the antimicrobial effects of silver nanoparticles (np) obtained by a new method and tested at concentrations of 6 μg/ml and 60 μg/ml against the species staphylococcus aureus, listeria innocua, salmonella choleraesuis, pseudomonas aeruginosa, escherichia ... | 2012 | 22488058 |
| survival of bacillus cereus vegetative cells and spores during in vitro simulation of gastric passage. | the enteric pathogen bacillus cereus must survive gastric passage in order to cause diarrhea by enterotoxin production in the small intestine. the acid resistance and the survival after gastric passage were assessed by in vitro experiments with acidified growth medium and gastric simulation medium with b. cereus nvh 1230-88 vegetative cells and spores. first, batch incubations at constant ph values for 4 h, which represented different physiological states of the stomach, showed that spores were ... | 2012 | 22488056 |
| [antibacterial activity of rare streptomyces species against clinical resistant bacteria]. | in the search for new antibiotics from steptomyces, investigating extremes habitats enhances the probability of isolating novel producers. in this context, the antibacterial activity of four streptomyces strains isolated from ezzmoul saltpans was studied. two of them showed antibacterial activity against antibiotic's resistant bacteria (bacillus cereus: β-lactamines and sulfamides resistant, streptococcus faecalis: penicillin, tetracycline and cotrimoxazole resistant, and staphylococcus aureus m ... | 2012 | 22484527 |
| phyto-synthesis of silver nanoscale particles using morinda citrifolia l. and its inhibitory activity against human pathogens. | leaf extract of morinda citrifolia l. was assessed for the synthesis of silver nanoscale particles under different temperature and reaction time. synthesized nanoscale (mcagnps) particles were confirmed by analysing the excitation of surface plasmon resonance (spr) using uv-visible spectrophotometer at 420 nm. further sem, hrtem analysis confirmed the range of particle size between 10 and 60 nm and sead pattern authorizes the face centered cubic (fcc) crystalline nature of the mcagnps. fourier t ... | 2012 | 22483838 |
| bacterial contamination of an automated pharmacy robot used for intravenous medication preparation. | multiple cultures were positive for bacillus cereus during routine quality assurance testing of a pharmacy robot that prepares intravenous medications. an investigation confirmed bacterial contamination of the robot as well as drug product made by the robot. the process and outcomes of the investigation are described in this report. | 2012 | 22476280 |
| bacteriophages bcp1-1 and bcp8-2 require divalent cations for efficient control of bacillus cereus in fermented foods. | bacillus cereus is a foodborne bacterial pathogen that causes diarrhea and vomiting. in this study, the usefulness of bacteriophages to eradicate b. cereus from fermented foods was investigated. a total of 13 phages were isolated from korean fermented food products, and 2 (bcp1-1 and bcp8-2) were further characterized. transmission electron microscopy (tem), restriction enzyme digestion pattern analysis, and sds-page of the structural proteins suggest that both phages belong to the family myovir ... | 2012 | 22475937 |
| characterization of two metal resistant bacillus strains isolated from slag disposal site at burnpur, india. | two strains of bacillus sp. resistant to arsenate and lead designated as assp9 and pbsp6, respectively were isolated from the slag disposal site. they were identified to be related to bacillus cereus cluster on the basis of 16s rdna based sequence analysis and phenotypic characteristics. both were rod-shaped (assp9, 2-5 microm and pbsp6, 2-4 microm), aerobic, salt tolerant (2-8% naci), endospore forming bacteria with minor differences like the assp9 showed sporangial bulging and pbsp6 had positi ... | 2011 | 22471215 |
| isolation and characterization of cr (vi) tolerant bacteria from tannery waste and its bioremediation potential. | the east kolkata wetland, situated in west bengal, india, is presently being exploited by housing many leather complexes surrounding the zone in and around. high amount of either untreated or partially treated liquid tannery wastes are discharged in the wetland. treated effluent from some common effluent treatment plant (cetp) showed the presence of cr (vi) exceeding the limiting concentration. three different bacterial strains (l1, l2, and l3) were isolated from the treated effluent. morphologi ... | 2012 | 24749383 |
| characterization of a spore-specific protein of the bacillus cereus group. | bc1245 is a monocistronic chromosomal gene of bacillus cereus atcc 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in b. cereus. bc1245 is highly conserved in the genome of members of the b. cereus group, indicating an important function of the gene in this group of bacteria. quantitative pcr revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell-specifi ... | 2012 | 22458449 |
| characterization of zinc-solubilizing bacillus isolates and their potential to influence zinc assimilation in soybean seeds. | one hundred thirty-four putative bacillus isolates were recovered from soybean rhizosphere soils of nimar region to select effective zinc solubilizers for increased assimilation of zinc (zn) in soybean seeds. these isolates were screened in vitro for zinc-solubilization ability on tris-minimal agar medium supplemented separately with 0.1% zinc in the form of zinc oxide, zinc phosphate, and zinc carbonate. of all, 9 isolates and a reference bacillus cereus atcc 13061 were characterized and identi ... | 2012 | 22450791 |
| structural analysis of the leptospiral sphingomyelinases: in silico and experimental evaluation of sph2 as an mg-dependent sphingomyelinase. | leptospiral sphingomyelinases are candidate virulence factors present only in pathogenic leptospira spp. leptospira interrogans serovar lai encodes sph1, sph2, sph3, sph4 and sphh. except for sph4, they all possess the exo-endo-phosphatase domain that groups them under the dnase i superfamily. | 2012 | 22441407 |
| impact of intestinal microbiota and gastrointestinal conditions on the in vitro survival and growth of bacillus cereus. | ingestion of b. cereus can result in diarrhea, if these bacteria survive gastrointestinal passage and achieve growth and enterotoxin production in the small intestine. the gastrointestinal survival of vegetative cells and spores of the diarrheal food poisoning strain b. cereus nvh 1230-88 was investigated during in vitro batch experiments simulating the stomach, duodenum and ileum using simulation media and competing intestinal microbiota. all spores and approx. 30% of the vegetative b. cereus c ... | 2012 | 22436640 |
| 'candidatus arthromitus' revised: segmented filamentous bacteria in arthropod guts are members of lachnospiraceae. | the name arthromitus has been applied collectively to conspicuous filamentous bacteria found in the hindguts of termites and other arthropods. first observed by joseph leidy in 1849, the identity of these filaments has remained contentious. while margulis and colleagues declared them to be a life stage of bacillus cereus, others have assumed them to belong to the same lineage as the segmented filamentous bacteria (sfb) from vertebrate guts, a group that has garnered much attention due to their u ... | 2012 | 22436008 |
| spectroscopic studies of the iron and manganese reconstituted tyrosyl radical in bacillus cereus ribonucleotide reductase r2 protein. | ribonucleotide reductase (rnr) catalyzes the rate limiting step in dna synthesis where ribonucleotides are reduced to the corresponding deoxyribonucleotides. class ib rnrs consist of two homodimeric subunits: r1e, which houses the active site; and r2f, which contains a metallo cofactor and a tyrosyl radical that initiates the ribonucleotide reduction reaction. we studied the r2f subunit of b. cereus reconstituted with iron or alternatively with manganese ions, then subsequently reacted with mole ... | 2012 | 22432022 |
| antiproliferative, antimicrobial and apoptosis inducing effects of compounds isolated from inula viscosa. | the antiproliferative and antimicrobial effects of thirteen compounds isolated from inula viscosa (l.) were tested in this study. the antiproliferative activity was tested against three cell lines using the mtt assay. the microdilution method was used to study the antimicrobial activity against two gram positive bacteria, two gram negative bacteria and one fungus. the apoptotic activity was determined using a tunel colorimetric assay. scanning electron microscopy was used to study the morphologi ... | 2012 | 22418930 |
| characterization of lysb4, an endolysin from the bacillus cereus-infecting bacteriophage b4. | bacillus cereus is a foodborne pathogen that causes emetic or diarrheal types of food poisoning. the incidence of b. cereus food poisoning has been gradually increasing over the past few years, therefore, biocontrol agents effective against b. cereus need to be developed. endolysins are phage-encoded bacterial peptidoglycan hydrolases and have received considerable attention as promising antibacterial agents. | 2012 | 22416675 |
| molecular and toxigenic characterization of bacillus cereus and bacillus thuringiensis strains isolated from commercial ground roasted coffee. | thirty samples of roasted ground coffee beans from 10 different commercial brands were analyzed to investigate the occurrence and levels of bacillus cereus and bacillus thuringiensis strains. strains were evaluated for their genetic diversity by repetitive element sequence polymorphism pcr (rep-pcr) and for their toxigenic profiles, i.e., the presence of hbla, hblc, hbld, nhea, nheb, nhec, cytk, ces, and entfm. survival and multiplication of b. cereus sensu lato in the ready-to-drink coffee was ... | 2012 | 22410226 |
| psychrotolerant paenibacillus tundrae isolates from barley grains produce new cereulide-like depsipeptides (paenilide and homopaenilide) that are highly toxic to mammalian cells. | paenilide is a novel, heat-stable peptide toxin from paenibacillus tundrae, which colonizes barley. p. tundrae produced 20 to 50 ng of the toxin mg(-1) of cells (wet weight) throughout a range of growth temperatures from +5°c to +28°c. paenilide consisted of two substances of 1,152 da and 1,166 da, with masses and tandem mass spectra identical to those of cereulide and a cereulide homolog, respectively, produced by bacillus cereus ns-58. the two components of paenilide were separated from those ... | 2012 | 22407690 |
| semisynthesis and antimicrobial activity of novel guttiferone-a derivatives. | six derivatives of guttiferone-a (lfqm-79, 80, 81, 82, 113 and 114) were synthesized and evaluated for their antimicrobial activity against the opportunistic or pathogenic fungi candida albicans (atcc 09548), candida glabrata (atcc 90030), candida krusei (atcc 6258), candida parapsilosis (atcc 69548), candida tropicalis (atcc 750), cryptococcus neoformans (atcc 90012), trichophyton tonsurans, microsporum gypseum and also against the opportunistic and pathogenic gram-positive bacteria staphylococ ... | 2012 | 22401914 |
| prevalence, phenotypic traits and molecular characterization of emetic toxin-producing bacillus cereus strains isolated from human stools in korea. | to investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing bacillus cereus strains isolated from sporadic food poisoning cases in korea. | 2012 | 22394210 |
| detection and expression of enterotoxin genes in endophytic strains of bacillus cereus. | the aim of this study was to determine whether endophytic bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (nhe) and haemolysin bl (hbl) and, therefore, have the potential to cause diarrhoeal illness in humans. | 2012 | 22394121 |
| inhibition of cytotoxicity by the nhe cytotoxin of bacillus cereus through the interaction of dodecyl maltoside with the nheb component. | nhe ('nonhaemolytic enterotoxin') is a three-component cytotoxin implicated in the pathogenesis of diarrhoea by bacillus cereus. nhe forms pores in pure lipid bilayers, but the function of the individual components (nhea, nheb and nhec) remains unclear. nheb and nhec are structural homologues of clya, a pore-forming cytotoxin of escherichia coli. the non-ionic detergent dodecyl maltoside (ddm) has been shown to inhibit haemolysis of clya. we used ddm as a probe to examine the response of the nhe ... | 2012 | 22385099 |
| x-ray absorption spectroscopy of metal site speciation in the metallo-β-lactamase bcii from bacillus cereus. | cobalt and zinc binding by the subclass b1 metallo-β-lactamase bcii from bacillus cereus is examined by x-ray absorption spectroscopy, at various levels of metal loading. the data show that a significant amount of the dinuclear enzyme is formed, even at substoichiometric levels of metal loading, whether the added metal is zn(ii) or co(ii). increasing metal addition, from 0.5 to 1.0 to 2.0eq/mol of enzyme, are shown to result in a more ordered active site. while zn(ii) appears to show no preferen ... | 2012 | 22381913 |
| occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment. | the occurrence of sulfonamide and tetracycline resistance and their pollution profile in the aquaculture environment of tianjin, northern china, were investigated. the presence of antibiotic-resistant bacteria was identified and the corresponding antibiotic resistance genes (args) were quantified at 6 aquaculture farms in tianjin. sulfonamide-resistance genes were prevalent and their concentrations were the highest detected (3.0 × 10(-5) to 3.3 × 10(-4) for sul1/16s rdna, 2.0 × 10(-4) to 1.8 × 1 ... | 2012 | 22377146 |
| complete genome sequence of the highly hemolytic strain bacillus cereus f837/76. | highly hemolytic strain bacillus cereus f837/76 was isolated in 1976 from a contaminated prostate wound. the complete nucleotide sequence of this strain reported here counts nearly 36,500 single-nucleotide differences from the closest sequenced strain, bacillus thuringiensis al hakam. f827/76 also contains a 10-kb plasmid that was not detected in the al hakam strain. | 2012 | 22374959 |
| [investigation of ampicillin effect on morphological and mechanical properties of escherichia coli and bacillus cereus cells with atomic force microscopy]. | the effect of subbacteriostatic concentrations of ampicillin on morphological and mechanical properties of gramnegative and grampositive cells of escherichia coli k12 tg1 and bacillus cereus ip 5832 respectively was studied with atomic force microscopy. significant heterogeneity of the bacterial populations was shown by the character of the response to the antibiotic effect. the common feature was increase of the cell size likely due to the effect of the inner osmotic pressure on the lowered cel ... | 2011 | 22359862 |
| bacteriostatic mode of action of trypsin-hydrolyzed palm kernel expeller peptide against bacillus cereus. | palm kernel expeller (pke), the by-product derived from the palm kernel oil milling industry, is commonly added to ruminant feed as a source of protein. recent research has demonstrated that the enzymatically hydrolyzed protein is inhibitory to spore-forming bacteria including bacillus cereus. the trypsin-hydrolyzed pke peptide appears to disrupt the membrane integrity and inhibit the intracellular macromolecule metabolism of b. cereus. the addition of the pke peptide (350 and 700 μg/ml) to b. c ... | 2012 | 26781737 |
| isolation and characterization of bacillus cereus ist105 from electroplating effluent for detoxification of hexavalent chromium. | electroplating industries are the main sources of heavy metals, chromium, nickel, lead, zinc, cadmium and copper. the highest concentrations of chromium (vi) in the effluent cause a direct hazards to human and animals. therefore, there is a need of an effective and affordable biotechnological solution for removal of chromium from electroplating effluent. | 2011 | 22351260 |
| bioactives of microbes isolated from western ghat belt of kerala show β-lactamase inhibition along with wide spectrum antimicrobial activity. | the present study describes the exploitation of microbial biodiversity from western ghats of kerala for screening of bioactives having β-lactamase inhibitory activities. a total of 700 pure cultures were isolated and were screened for antibacterial activity against a β-lactam resistant bacillus cereus strain (pl 10) isolated from the same niche. bioactive extracts made from 45 isolates showed inhibitory activities against pl 10, of which two strains showed inhibition of extended spectrum β-lacta ... | 2012 | 22350938 |
| volatile organic compounds from centaurium erythraea rafn (croatia) and the antimicrobial potential of its essential oil. | gc and ms were used for the analysis of croatian centaurium erythraea rafn essential oil (obtained by hydrodistillation) and headspace (applying headspace solid-phase microextraction). the headspace contained numerous monoterpene hydrocarbons (the major ones were terpinene-4-ol, methone, p-cymene, γ-terpinene and limonene). oxygenated monoterpenes were present in the headspace and oil, while 1,8-cineole, bornyl acetate and verbenone were present only in the headspace. high headspace percentages ... | 2012 | 22349896 |
| bacillus cereus catheter related bloodstream infection in a patient with acute lymphoblastic leukemia. | bacillus cereus infection is rarely associated with actual infection and for this reason single positive blood culture is usually regarded as contamination . however it may cause a number of infections, such catheter-related bloodstream infections. significant catheter-related bloodstream infections (crbsi) caused by bacillus spp. are mainly due to b. cereus and have been predominantly reported in immunocompromised hosts. catheter removal is generally advised for management of infection. in this ... | 2012 | 22348186 |
| seasonal variations of phenolic compounds and biological properties in sage (salvia officinalis l.). | the aim was to investigate the phenolic content, antioxidant capacity, and antibacterial activity of dalmatian sage (salvia officinalis l.) leaves collected during different vegetation periods. separation and quantification of the individual phenols were performed by reversed-phase (rp)-hplc coupled with a pda (photodiode array) detector and using an internal standard, while the contents of total phenols, flavonoids, flavones, and flavonols were determined spectrophotometrically. the antioxidant ... | 2012 | 22344920 |
| molecular differences between a mutase and a phosphatase: investigations of the activation step in bacillus cereus phosphopentomutase. | prokaryotic phosphopentomutases (ppms) are di-mn(2+) enzymes that catalyze the interconversion of α-d-ribose 5-phosphate and α-d-ribose 1-phosphate at an active site located between two independently folded domains. these prokaryotic ppms belong to the alkaline phosphatase superfamily, but previous studies of bacillus cereus ppm suggested adaptations of the conserved alkaline phosphatase catalytic cycle. notably, b. cereus ppm engages substrates when the active site nucleophile, thr-85, is phosp ... | 2012 | 22329805 |
| application of atmospheric pressure nonthermal plasma for the in vitro eradication of bacterial biofilms. | the use of atmospheric pressure nonthermal plasma represents an interesting and novel approach for the decontamination of surfaces colonized with microbial biofilms that exhibit enhanced tolerance to antimicrobial challenge. in this study, the influence of an atmospheric pressure nonthermal plasma jet, operated in a helium and oxygen gas mixture under ambient pressure, was evaluated against biofilms of bacillus cereus, staphylococcus aureus, escherichia coli and pseudomonas aeruginosa. within < ... | 2012 | 22329678 |
| bacillus cytotoxicus sp. nov. is a novel thermotolerant species of the bacillus cereus group occasionally associated with food poisoning. | an aerobic endospore-forming bacillus (nvh 391-98(t)) was isolated during a severe food poisoning outbreak in france in 1998, and four other similar strains have since been isolated, also mostly from food poisoning cases. based on 16s rrna gene sequence similarity, these strains were shown to belong to the bacillus cereus group (over 97% similarity with the current group species) and phylogenetic distance from other validly described species of the genus bacillus was less than 95%. based on 16s ... | 2013 | 22328607 |
| enhancement of cypermethrin degradation by a coculture of bacillus cereus zh-3 and streptomyces aureus hp-s-01. | degradation of cypermethrin was significantly enhanced in a coculture of bacillus cereus zh-3 and streptomyces aureus hp-s-01. in the pure culture, longer half-lives (t(1/2)=32.6-43.0h) of cypermethrin were observed, as compared to the mixed cocultures (t(1/2)=13.0h). the optimal degradation conditions were determined to be 28.2°c and ph 7.5 based on response surface methodology (rsm). under these conditions, the mixed cultures completely metabolized cypermethrin (50mgl(-1)) within 72h. analysis ... | 2012 | 22326328 |
| bioaugmentation for polyacrylamide degradation in a sequencing batch reactor and contact oxidation reactor. | in the present study, one pam degrading bacterial strain, originally named hwbi, was isolated from an activated sludge sample and used as an exogenous bacteria for bioaugmentation. the strain was primarily identified as bacillus cereus. one contact oxidation reactor (cor) and one sequencing batch reactor (sbr) were bioaugmented with the hwbi, respectively, and the performance of the bioaugmented systems for pam removal were investigated under long term operation. results showed that for the cor ... | 2012 | 22320687 |
| comparison of alkyl hydroperoxide reductase and two water-forming nadh oxidases from bacillus cereus atcc 14579. | bacillus cereus (b. cereus) is an ubiquitous facultative anaerobic bacterium, and its growth in aerobic environment correlates to the functions of its oxygen defense system. water-forming nadh oxidase (nox-2) can catalyze the conversion of oxygen to water with concomitant nadh oxidation in anaerobic microorganisms. here, we report the cloning and characterization of two annotated nox-2 s (nox-2(444) and nox-2(554)) from b. cereus atcc 14579 and their comparison with another oxidative stress defe ... | 2012 | 22311647 |
| functional assignment of yvgo, a novel set of purified and chemically characterized proteinaceous antifungal variants produced by bacillus thuringiensis sf361. | this study reports a novel class of antifungal protein derived from bacterial origin. bacillus thuringiensis sf361, the strain also responsible for producing the novel bacteriocin thurincin h, exhibits broad antifungal activity against select members of several fungal genera, including aspergillus, byssochlamys, and penicillium, as well as the pathogenic yeast candida albicans. optimal antifungal production and secretion were observed after-log phase growth when incubated at 37°c in a carbohydra ... | 2012 | 22307285 |
| temperature-dependent production of various plcr-controlled virulence factors in bacillus weihenstephanensis strain kbab4. | the bacillus cereus sensu lato complex has recently been divided into several phylogenetic groups with clear differences in growth temperature range. however, only a few studies have investigated the actual pathogenic potential of the psychrotolerant strains of the b. cereus group at low temperature, and little information is available concerning gene expression at low temperature. we found that vegetative cells of the psychrotolerant b. weihenstephanensis strain kbab4 were pathogenic against th ... | 2012 | 22307282 |
| antimicrobial activity and essential oils of curcuma aeruginosa, curcuma mangga, and zingiber cassumunar from malaysia. | to analyze the chemical composition of the essential oils of curcuma aeruginosa (c. aeruginosa), curcuma mangga (c. mangga), and zingiber cassumunar (z. cassumunar), and study their antimicrobial activity. | 2012 | 22305785 |
| evaluation of the sterility testing process of hematopoietic stem cells at canadian blood services. | sterility testing of hematopoietic stem cells (hscs) at the canadian blood services stem cell laboratory is performed using bact/alert aerobic (sa) culture bottles. this study was conducted to verify the efficacy of this method and to assess the use of the bact/alert aerobic (bpa) and anaerobic (bpn) culture bottles for microbial testing of hscs. | 2012 | 22304655 |
| mechanism and diversity of the erythromycin esterase family of enzymes. | macrolide antibiotics such as azithromycin and erythromycin are mainstays of modern antibacterial chemotherapy, and like all antibiotics, they are vulnerable to resistance. one mechanism of macrolide resistance is via drug inactivation: enzymatic hydrolysis of the macrolactone ring catalyzed by erythromycin esterases, erea and ereb. a genomic enzymology approach was taken to gain insight into the catalytic mechanisms and origins of ere enzymes. our analysis reveals that erythromycin esterases co ... | 2012 | 22303981 |
| novel bacterial lipoprotein structures conserved in low-gc content gram-positive bacteria are recognized by toll-like receptor 2. | bacterial lipoproteins/lipopeptides inducing host innate immune responses are sensed by mammalian toll-like receptor 2 (tlr2). these bacterial lipoproteins are structurally divided into two groups, diacylated or triacylated lipoproteins, by the absence or presence of an amide-linked fatty acid. the presence of diacylated lipoproteins has been predicted in low-gc content gram-positive bacteria and mycoplasmas based on the absence of one modification enzyme in their genomes; however, we recently d ... | 2012 | 22303020 |
| iodine-stabilized cu nanoparticle chitosan composite for antibacterial applications. | we report herein the synthesis of a new composite consisting of cu nanoparticles (nps) and chitosan (cs), which has been found to be stable in the presence of molecular iodine and has also high antimicrobial activities. the composite could be obtained when aqueous cuso(4) was treated with hydrazine in the presence of cs. the spherical cu nps present in the composite were of average diameters 8±4 nm. the nps were unstable in atmospheric conditions leading to the formation of oxides of cu. on the ... | 2012 | 22301575 |
| purification, crystallization and preliminary x-ray characterization of bacillus cereus arylamine n-acetyltransferase 3 [(baccr)nat3]. | arylamine n-acetyltransferases (nats) are xenobiotic metabolizing enzymes (xmes) that catalyze the acetylation of arylamines. all functional nats described to date possess a strictly conserved cys-his-asp catalytic triad. here, the purification, crystallization and preliminary x-ray characterization of bacillus cereus arylamine n-acetyltransferase 3 [(baccr)nat3], a putative nat isoenzyme that possesses a unique catalytic triad containing a glutamate residue, is reported. the crystal diffracted ... | 2012 | 22297998 |
| bacterial mixture from greenhouse soil as a biocontrol agent against root-knot nematode, meloidogyne incognita, on oriental melon. | the biological control efficacy of a greenhouse soil bacterial mixture of lactobacillus farraginis, bacillus cereus, and bacillus thuringiensis strains with antinematode activity was evaluated against the root-knot nematode meloidogyne incognita. two control groups planted in soil drenched with sterile distilled water or treated with the broadspectrum carbamate pesticide carbofuran were used for comparison. the results suggest that the bacterial mixture is effective as a biocontrol agent against ... | 2012 | 22297227 |
| genetic diversity, antimicrobial resistance, and toxigenic profiles of bacillus cereus strains isolated from sunsik. | bacillus cereus can cause emetic and diarrheal types of food poisoning, but little study has been done on the toxins and toxin-encoding genes of b. cereus strains isolated from sunsik, a korean ready-to-eat food prepared from grains, fruits, and vegetables. in this study, 39 unique b. cereus strains were isolated and identified from sunsik samples, with an average contamination level of 10 to 200 cfu/g. the detection rates of the hblacd, cytk, and bcet genes among all the strains were 48.7, 66.7 ... | 2012 | 22289581 |
| the additive and synergistic antimicrobial effects of select frankincense and myrrh oils--a combination from the pharaonic pharmacopoeia. | the in vitro antimicrobial activity of three essential oil samples of frankincense (boswellia rivae, boswellia neglecta and boswellia papyrifera) and two essential oil samples of myrrh and sweet myrrh (commiphora guidotti and commiphora myrrha), collected from different regions of ethiopia, was investigated independently and in combination to determine their anti-infective properties. | 2012 | 22288378 |
| evidence of bacillus thuringiensis intra-serovar diversity revealed by bacillus cereus group-specific repetitive extragenic palindromic sequence-based pcr genomic fingerprinting. | bacillus thuringiensis is classified into serovars on the basis of h-flagellar antigens. several alternative typing methods have been described. among them, a b. cereus group-specific repetitive extragenic palindromic (rep)-pcr fingerprinting technique was shown to be discriminative and able to identify b. thuringiensis serovars. the aim of this study was to investigate the genomic diversity and relationship among b. thuringiensis strains collected from different argentinean ecosystems. thirty-s ... | 2011 | 22286045 |
| the immune modulation of bacillus cereus var. toyoi in mice immunized with experimental inactivated bovine herpesvirus type 5 vaccine. | in recent years, there been significant progress toward develop more efficient vaccines. different compounds with adjuvant capacity have been tested; however, no compound has emerged that suitable for universal use. several efforts have been made to produce effective vaccines against bovine herpesvirus 5 (bohv-5), an important cattle pathogen. in this study we examine the use the probiotic bacillus cereus var. toyoi as a potential adjuvant to improve bohv-5 vaccine efficacy. we observed in the s ... | 2012 | 22285271 |
| assessment of microbial quality of commercial and home-made tiger-nut beverages. | we aimed to assess the microbiological quality of spanish commercial tiger-nut beverages as well as home-made samples collected from supermarkets, street vendors, juice bars and ice-cream parlours located in valencia. | 2012 | 22283438 |
| isolation and growth characteristics of chromium(vi) and pentachlorophenol tolerant bacterial isolate from treated tannery effluent for its possible use in simultaneous bioremediation. | the bacterial strains resistant to pentachlorophenol (pcp) and hexavalent chromium [cr(vi)] were isolated from treated tannery effluent of a common effluent treatment plant. most of the physico-chemical parameters analyzed were above permissible limits. thirty-eight and four bacterial isolates, respectively were found resistant to >50 μg/ml concentration of [cr(vi)] and the same level of pcp. out of the above 42 isolates, only one was found simultaneously tolerant to higher levels of both pcp (5 ... | 2011 | 22282630 |
| bacillus cereus can attack the cell membranes of the alga chara corallina by means of hlyii. | we studied the influence of bacillus cereus bacteria on cells of the freshwater alga chara corallina. these bacteria and recombinant bacillus subtilis strains are capable of producing the secreted toxin hlyii, which changes the electrophysiological parameters of the algal electrically excitable plasma membrane by forming pores. cooperative incubation of bacterial cells, which carry active hlyii gene, and chara corallina cells caused a decrease in the resting potential (v(m)) and plasma membrane ... | 2012 | 22281415 |
| milk microbiome signatures of subclinical mastitis-affected cattle analysed by shotgun sequencing. | metagenomic analysis of milk samples collected from kankrej, gir (bos indicus) and crossbred (bos taurus × b. indicus) cattle harbouring subclinical mastitis was carried out by next-generation sequencing 454 gs-flx technology to elucidate the microbial community structure of cattle milk. | 2012 | 22277077 |
| influence of anaerobiosis and low temperature on bacillus cereus growth, metabolism, and membrane properties. | the impact of simultaneous anaerobiosis and low temperature on growth parameters, metabolism, and membrane properties of bacillus cereus atcc 14579 was studied. no growth was observed under anaerobiosis at 12°c. in bioreactors, growth rates and biomass production were drastically reduced by simultaneous anaerobiosis and low temperature (15°c). the two conditions had a synergistic effect on biomass reduction. in anaerobic cultures, fermentative metabolism was modified by low temperature, with a m ... | 2012 | 22247126 |
| critical illness polyneuropathy and myopathy caused by bacillus cereus sepsis in acute lymphoblastic leukemia. | we report a pediatric case of critical illness polyneuropathy and myopathy caused by bacillus cereus sepsis during acute lymphoblastic leukemia therapy. a 15-year-old boy developed b. cereus sepsis and multiple organ failure on the 19th day after initiation of chemotherapy, and multidisciplinary treatment was started. treatment was effective and septic shock with multiple organ failure remitted. he was weaned from a respirator on day 23 after the onset of sepsis, but complete flaccid paralysis o ... | 2012 | 22246161 |
| bacillus cereus, an unusual cause of fulminant liver failure: diagnosis may prevent liver transplantation. | bacillus cereus is a well-known cause of foodborne disease usually of benign course. here, we present the case of a 15-year-old boy who developed reversible fulminant liver failure associated with rhabdomyolysis after pasta consumption. suspecting b. cereus as the aetiological agent may prevent unnecessary liver transplantation. | 2012 | 22245788 |
| cereulide produced by bacillus cereus increases the fitness of the producer organism in low-potassium environments. | cereulide, produced by certain bacillus cereus strains, is a lipophilic cyclic peptide of 1152 da that binds k(+) ions with high specificity and affinity. it is toxic to humans, but its role for the producer organism is not known. we report here that cereulide operates for b. cereus to scavenge potassium when the environment is growth limiting for this ion. cereulide-producing b. cereus showed higher maximal growth rates (µ(max)) than cereulide non-producing b. cereus in k(+)-deficient medium (k ... | 2012 | 22241046 |
| microbial biodiversity, quality and shelf life of microfiltered and pasteurized extended shelf life (esl) milk from germany, austria and switzerland. | information on factors limiting the shelf life of extended shelf life (esl) milk produced by microfiltration and subsequent pasteurization is very limited. in this study, three different batches of esl milk were analyzed at different stages of the production process and during storage at 4 °c, 8 °c and 10 °c in order to evaluate the changes in bacterial cell counts, microbial diversity and enzymatic quality. additionally, detailed biodiversity analyses of 250 retail esl milk packages produced by ... | 2012 | 22240060 |
| antimicrobial prenylated acetophenones from berries of harrisonia abyssinica. | the ripe berries of harrisonia abyssinica yielded two new prenylated acetophenones, namely 5-(ethan-1'''-one)-4,6-dihydroxy-7-(3'',3''-dimethylallyl)-2 s-(1' s-hydroxy-1',5'-dimethylhex-4'-enyl)-2,3-dihydrobenzofuran (1) and 5-(2'''-hydroxyethan-1'''-one)-4,6-dihydroxy-7-(3'',3''-dimethylallyl)-2 s-(1' s-hydroxy-1',5'-dimethylhex-4'-enyl)-2,3-dihydrobenzofuran (2), herein named harronin i and harronin ii, respectively. the compounds were isolated following activity guided fractionation and the s ... | 2012 | 22234410 |
| a comparison of the effects of e-beam irradiation and heat treatment on the variability of bacillus cereus inactivation and lag phase duration of surviving cells. | the effects of electron beam irradiation and heat treatments on the variability of inactivation of bacillus cereus spores (cect 131/atcc 10876) and of the lag phase of single surviving cells have been studied. in general, dispersion in the number of survivors increased as the stress became more intense. a polynomial relationship was derived between the coefficient of variation of the survivor number and the inactivation achieved. heat treatments caused wider distributions than irradiation for th ... | 2012 | 22225985 |
| cr(vi) uptake mechanism of bacillus cereus. | in this study, we investigated the cr(vi) uptake mechanism in an indigenous cr(vi)-tolerant bacterial strain -bacillus cereus through batch and microscopic experiments. we found that both the cells and the supernatant collected from b. cereus cultivation could reduce cr(vi). the valence state analysis revealed the complete transformation from cr(vi) into cr(iii) by living b. cereus. further x-ray absorption fine structure and fourier transform infrared analyses showed that the reduced cr(iii) wa ... | 2012 | 22225704 |
| regulation of xylanase biosynthesis in bacillus cereus bsa1. | microbial xylanases have a promising biotechnological potential to be used in industries. in this study, regulation of xylanase production was examined in bacillus cereus bsa1. xylanase production was induced by xylan. the enzyme production further increased in the presence of xylose and arabinose in very low concentration with addition of xylan (0.5% up to 6.02 u/ml). addition of glucose (about 0.1%) to the media supplemented with xylan repressed xylanase production. even higher concentration ( ... | 2012 | 22222433 |
| isolation, partial purification and evaluation of bioactive compounds from leaves of ageratum houstonianum. | the present study deals with the isolation and partial purification of bioactive compounds from the crude methanol extracts of the leaves of ageratum houstonianum (asteraceae). the quantification and the identification of compounds in the crude extract and active bands isolated by preparative tlc were accomplished using gc-ms analysis. the most important compounds identified in the crude extract and active bands (ab-1 and ab-2) were 6-acetyl-7-methoxy-2, 2-dimethylchromene, hexadecanoic acid and ... | 2012 | 27350770 |
| electrochemical biosensor for rapid and sensitive detection of magnetically extracted bacterial pathogens. | biological defense and security applications demand rapid, sensitive detection of bacterial pathogens. this work presents a novel qualitative electrochemical detection technique which is applied to two representative bacterial pathogens, bacillus cereus (as a surrogate for b. anthracis) and escherichia coli o157:h7, resulting in detection limits of 40 cfu/ml and 6 cfu/ml, respectively, from pure culture. cyclic voltammetry is combined with immunomagnetic separation in a rapid method requiring ap ... | 2012 | 25585629 |
| invasive bacillus cereus infection in a renal transplant patient: a case report and review. | bacillus cereus is a common cause of gastrointestinal diseases. the majority of individuals with b cereus-related food poisoning recover without any specific treatment. it can, however, rarely cause invasive disease in immunocompromised patients. | 2012 | 24294281 |
| synthesis, sar and antibacterial activity of hybrid chloro, dichloro-phenylthiazolyl-s-triazines. | a series of hybrid novel chloro (1a-9a) and dichloro (10b-18b) phenylthiazolyl-s-triazine were synthesized and subsequently subjected to their antibacterial activity against three gram positive viz. lactobacillus casei (ncim-2651); bacillus cereus (ncim-2458); staphylococcus aureus (ncim-2120) and three gram negative viz salmonella typhimurium (ncim-2501); escherichia coli (ncim-2065); klebsiella aerogenes (ncim-2098). the sar studies around the lead compound revealed that introduction of electr ... | 2012 | 23960775 |
| naturally occurring medicinal mushroom-derived antimicrobials: a case-study using lingzhi or reishi ganoderma lucidum (w. curt.:fr.) p. karst. (higher basidiomycetes). | antibacterial activity of lingzhi or reishi medicinal mushroom, ganoderma lucidum, collected from central india was evaluated against four bacterial pathogens. ethyl alcohol and water extracts of fruit body powder were tested using the disc diffusion method against staphylococcus aureus, klebsiella pneumoniae, bacillus cereus, and pseudomonas aeruginosa. it was noted that the aqueous extract inhibited growth of pathogenic bacteria. the combined effect of fruit body extract with synthetic antibio ... | 2012 | 23510217 |