| Furfural reduction mechanism of a zinc-dependent alcohol dehydrogenase from Cupriavidus necator JMP134. | FurX is a tetrameric Zn-dependent alcohol dehydrogenase (ADH) from Cupriavidus necator JMP134. The enzyme rapidly reduces furfural with NADH as the reducing power. For the first time among characterized ADHs, the high-resolution structures of all reaction steps were obtained in a time-resolved manner, thereby illustrating the complete catalytic events of NADH-dependent reduction of furfural and the dynamic Zn(2+) coordination among Glu66, water, substrate and product. In the fully closed confor ... | 2012 | 22081946 |
| recovery and purification of intracellular polyhydroxyalkanoates from recombinant cupriavidus necator using water and ethanol. | a new halogen-free and environmental-friendly method using water and ethanol is developed as an alternative for the recovery of polyhydroxyalkanoates (pha) from recombinant cupriavidus necator in comparison to the established chloroform extraction method. after optimisation, our results showed that the halogen-free method produced a pha with 81% purity and 96% recovery yield, in comparison to the chloroform extraction system which resulted in a highly pure pha with 95% yield. although the purity ... | 2011 | 22038551 |
| spectroscopic characterization of the metal-binding sites in the periplasmic metal-sensor domain of cnrx from cupriavidus metallidurans ch34. | cnrx, the dimeric metal sensor of the three-protein transmembrane signal transduction complex cnryxh of cupriavidus metallidurans ch34, contains one metal-binding site per monomer. both ni and co elicit a biological response and bind the protein in a 3n2o1s coordination sphere with a nearly identical octahedral geometry as shown by the x-ray structure of cnrxs, the soluble domain of cnrx. however, in solution cnrxs is titrated by 4 co-equiv and exhibits an unexpected intense band at 384 nm that ... | 2011 | 21942751 |
| biosynthesis and characterization of polyhydroxyalkanoate block copolymer p3hb-b-p4hb. | polyhydroxyalkanoates (pha) synthesis genes phbc and orfz cloned from ralstonia eutropha h16 were transformed into beta-oxidation weakened pseudomonas putida ktoy08δgc, a mutant of p. putida kt2442. the recombinant p. putida strain termed kthh06 was able to produce a short-chain-length pha block copolymer consisting of poly(3-hydroxybutyrate) (p3hb) as one block and poly(4-hydroxybutyrate) (p4hb) as another block. one-dimensional and two-dimensional nuclear magnetic resonance (nmr) clearly indic ... | 2011 | 21863836 |
| production of polyhydroxyalkanoates from waste frying oil by cupriavidus necator. | abstract: polyhydroxyalkanoates (phas) are biopolymers, which can replace petrochemical plastics in many applications. however, these bioplastics are currently far more expensive than petrochemical plastics. many researchers are investigating the use of inexpensive substrates derived from waste streams. waste frying oil is abundant and can be used in pha production without filtration.cupriavidus necator (formerly known as ralstonia eutropha) is a versatile organism for the production of phas. sm ... | 2011 | 21906352 |
| sulfoquinovose degraded by pure cultures of bacteria with release of c(3) -organosulfonates: complete degradation in two-member communities. | sulfoquinovose (sq, 6-deoxy-6-sulfoglucose) was synthesized chemically. an hplc-elsd method to separate sq and other chromophore-free sulfonates, e.g. 2,3-dihydroxypropane-1-sulfonate (dhps), was developed. a set of 10 genome-sequenced, sulfonate-utilizing bacteria did not utilize sq, but an isolate, pseudomonas putida sq1, from an enrichment culture did so. the molar growth yield with sq was half of that with glucose, and 1 mol 3-sulfolactate (mol sq)(-1) was formed during growth. the 3-sulfol ... | 2011 | 22150877 |
| Microalgae as bioreactors for bioplastic production. | Poly-3-hydroxybutyrate (PHB) is a polyester with thermoplastic properties that is naturally occurring and produced by such bacteria as Ralstonia eutropha H16 and Bacillus megaterium. In contrast to currently utilized plastics and most synthetic polymers, PHB is biodegradable, and its production is not dependent on fossil resources making this bioplastic interesting for various industrial applications. | 2011 | 22004563 |
| predicting metabolic pathways by sub-network extraction. | various methods result in groups of functionally related genes obtained from genomes (operons, regulons, syntheny groups, and phylogenetic profiles), transcriptomes (co-expression groups) and proteomes (modules of interacting proteins). when such groups contain two or more enzyme-coding genes, graph analysis methods can be applied to extract a metabolic pathway that interconnects them. we describe here the way to use the pathway extraction tool available on the neat web server ( ... | 2012 | 22144151 |
| Microbial degradation of furanic compounds: biochemistry, genetics, and impact. | Microbial metabolism of furanic compounds, especially furfural and 5-hydroxymethylfurfural (HMF), is rapidly gaining interest in the scientific community. This interest can largely be attributed to the occurrence of toxic furanic aldehydes in lignocellulosic hydrolysates. However, these compounds are also widespread in nature and in human processed foods, and are produced in industry. Although several microorganisms are known to degrade furanic compounds, the variety of species is limited mostly ... | 2011 | 22031465 |
| Aromatic compounds degradation plays a role in colonization of Arabidopsis thaliana and Acacia caven by Cupriavidus pinatubonensis JMP134. | Plant rhizosphere and internal tissues may constitute a relevant habitat for soil bacteria displaying high catabolic versatility towards xenobiotic aromatic compounds. Root exudates contain various molecules that are structurally related to aromatic xenobiotics and have been shown to stimulate bacterial degradation of aromatic pollutants in the rhizosphere. The ability to degrade specific aromatic components of root exudates could thus provide versatile catabolic bacteria with an advantage for r ... | 2011 | 22186997 |
| Aerobic pretreatment of olive oil mill wastewater using Ralstonia eutropha. | Olive oil mill wastewater (OMW) has a high polluting power, with total phenolics (TP) around 2.5 g l(-1) and chemical oxygen demand (COD) 85 g l(-1). Biological systems offer advantages in treating this type of agro-industrial wastewater. The performance of phenol-adapted Ralstonia eutropha for aerobic biotreatment of OMW has been studied, and a TP concentration of 250 mg l(-1) found to be fully degraded within 24 h. This simple procedure may be adopted as a pretreatment prior to the normal aero ... | 2011 | 21882561 |
| A new DNA extraction method by controlled alkaline treatments from consolidated subsurface sediments. | Microbial communities that thrive in subterranean consolidated sediments are largely unknown owing to the difficulty of extracting DNA. As this difficulty is often attributed to DNA binding onto the silica-bearing sediment matrix, we developed a DNA extraction method for consolidated sediment from the deep subsurface in which silica minerals were dissolved by being heated under alkaline conditions. NaOH concentrations (0.07 and 0.33 N), incubation temperatures (65 and 94 °C) and incubation times ... | 2012 | 22092362 |
| burkholderia and cupriavidus spp. are the preferred symbionts of mimosa spp. in southern china. | rhizobia were isolated from invasive mimosa spp. (m. diplotricha and m. pudica) in dehong district of the province of yunnan in subtropical southern china. almost all of the 98 isolates were β-rhizobia in the genera burkholderia and cupriavidus. these strains were analyzed for their distribution characteristics together with strains from a previous study from sishuangbanna. the proportion of nodules containing each β-rhizobial genus varied between mimosa species, with cupriavidus being predomina ... | 2012 | 22268711 |
| new beta rhizobial strains able to efficiently nodulate parapiptadenia rigida (benth.) brenan. | among the leguminous trees natives to uruguay parapiptadenia rigida (angico), a mimosoideae legume, is one of the most promising species for agroforestry. like many other legumes, it is able to establish symbiotic associations with rhizobia and belongs to the group known as "nitrogen fixing trees", which are major components of agroforestry systems. information about rhizobial symbionts for this genus is scarce, and thus the aim of this work was to identify and characterize rhizobia associated w ... | 2012 | 22226956 |