| [analysis on 2175 admitted cases of clonorchiasis sinensis in guigang city]. | to understand the epidemiological features of the hospitalized cases with clonorchis sinensis infection in guigang city of guangxi region. | 2008 | 19157304 |
| [effect of tribendimidine, artesunate, artemether and praziquantel, administered intragastrically at single, multiple or combined doses, to rats infected with clonorchis sinensis]. | to assess the efficacy of single, multiple or combined oral doses of tribendimidine, artesunate, artemether and praziquantel against clonorchis sinensis in rats. | 2008 | 19160575 |
| changes in sonographic findings after treatment of patients with clonorchiasis in a heavy endemic area. | we measured changes in sonographic findings of patients with clonorchiasis after a treatment in a highly endemic area. a total of 347 residents showed positive stool results for clonorchis sinensis eggs in a village in northeastern china, and were treated with praziquantel. of them, 132 patients underwent abdominal sonography both before and 1 year after treatment, and the changes in sonographic findings of 83 cured subjects were compared. diffuse dilatation of intrahepatic bile ducts (ddihd) wa ... | 2009 | 19290087 |
| a nationwide survey on the prevalence of intestinal parasitic infections in the republic of korea, 2004. | national surveys on the prevalence of intestinal parasitic infections have been carried out every 5-7 years since 1971 in the republic of korea in order to establish control measures. the present nationwide survey was conducted from june to december 2004. the 10% population sampling data of population and housing census by the korean government in 2000 was used as the survey population. one sample was selected randomly from each of the 22,858 registered subjects, and a total of 20,541 people wer ... | 2009 | 19290090 |
| [excretory/secretory antigens from clonorchis sinensis induces hepatic fibrosis in rats]. | to investigate the role of excretory/secretory antigens from clonorchis sinensis (csesas) in hepatic fibrosis induced by c. sinensis infection in rats and explore the possible mechanism. | 2009 | 19304508 |
| cathepsin f cysteine protease of the human liver fluke, opisthorchis viverrini. | the liver fluke opisthorchis viverrini is classified as a class i carcinogen due to the association between cholangiocarcinoma and chronic o. viverrini infection. during its feeding activity within the bile duct, the parasite secretes several cathepsin f cysteine proteases that may induce or contribute to the pathologies associated with hepatobiliary abnormalities. | 2009 | 19308250 |
| molecular cloning and analysis of stage and tissue-specific expression of cathepsin l-like protease from clonorchis sinensis. | cathepsin l of parasite plays multiple roles in growth, food uptake, and invasion into host and pathogenesis, which makes it a valuable target for diagnosis, vaccine, and drug. in this study, we identified a cdna encoding cathepsin l homolog (cscpl) from the library of clonorchis sinensis adult by bioinformatics analysis. sequence encoding proenzyme of cscpl (removal of signal peptide, csprocpl) was highly expressed in form of inclusion body in escherichia coli, and soluble rcsprocpl (about 1 mg ... | 2009 | 19308452 |
| serodiagnostic applicability of recombinant antigens of clonorchis sinensis expressed by wheat germ cell-free protein synthesis system. | we evaluated the diagnostic applicability of recombinant proteins from clonorchis sinensis, the human liver fluke. four recombinant proteins, 7-kda protein (cs7p), 28-kda cysteine protease (cs28cp), and 26- and 28-kda glutathione s-transferases (cs26gst and cs28gst), were expressed by wheat germ cell-free protein synthesis system. in elisa, crude antigen showed the highest sensitivity (92.7%). however, sensitivities of r7p (47.3%), r28cp (30.9%), r26gst (21.8%), and r28gst (14.5%) were dramatica ... | 2009 | 19376673 |
| [experimental study progress on tribendimidine, artemether and artesunate against clonorchis sinensis and other trematodes]. | currently praziquantel is one of the major drugs used in treatment of schistosomiasis and other trematode infections. recent experimental studies indicate that a new anthelmintic, tribendimidine, is used in the treatment of intestinal nematodes, also possesses effect against several species of trematodes including clonorchis sinensis, opisthorchis viverrini and echinostoma caproni. tribendimidine is even more effective against c. sinensis in rats that a single 300 mg/kg oral dose cures almost al ... | 2009 | 19459505 |
| molecular characterization of a novel clonorchis sinensis secretory phospholipase a(2) and investigation of its potential contribution to hepatic fibrosis. | a gene encoding a homologue of phospholipase a(2) was identified from the clonorchis sinensis adult cdna plasmid library. the deduced amino acid sequence including a signal peptide that has 28-46% identity with secretory phospholipase a(2), group iii (group iii spla(2)) of other species. it also has typical features of group iii spla(2)s including 10 cysteines, the key residues of the ca(2+) loop and catalytic site. the recombinant protein encoded by this gene expressed in escherichia coli showe ... | 2009 | 19463858 |
| single nucleotide polymorphisms of cytokine genes are associated with fibrosis of the intrahepatic bile duct wall in human clonorchiasis. | this study examined the association of cytokine gene polymorphisms with intrahepatic bile duct wall fibrosis in human clonorchiasis. a total of 240 residents in heilongjiang, china underwent ultrasonography, blood sampling, and stool examination. single nucleotide polymorphism (snp) sites for ifn-gamma (+874 t/a), il-10 (-1,082 g/a, -819 c/t, -592 c/a), tnf-alpha (-308 g/a), and tgf-beta1 (codon 10 t/c, codon 25 g/c) genes were observed with the taqman allelic discrimination assay. no significan ... | 2009 | 19488421 |
| identification of two beta-tubulin isotypes of clonorchis sinensis. | the beta-tubulin coding sequences were investigated for the chinese liver fluke, clonorchis sinensis. a cdna library of adult c. sinensis was screened with cdna probes synthesized by polymerase chain reaction using degenerate primers, and two beta-tubulin cdnas, cstb1 and cstb3, were subsequently cloned. the cstb1 and cstb3 cdna were 2,082 and 1,486 bp long and encoded 445 and 444 amino acids, respectively. the two clones were identical 78% in their coding sequences and 97% in deduced sequences. ... | 2009 | 19513750 |
| discovery of opisthorchis viverrini metacercariae in freshwater fish in southern cambodia. | small liver flukes, clonorchis sinensis and opisthorchis viverini, are fish-borne trematodes (fbts) causing significant public health problems in asia. while c. sinensis is distributing mainly in far east asia, o. viverini is distributing in indochina peninsula. recently, however, the geographical distributions of those small liver flukes were proven to be far wider than expected. nevertheless, little is known about the epidemiology of small liver flukes in cambodia. the present study is, theref ... | 2009 | 19524077 |
| identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory-secretory products of clonorchis sinensis adult worms. | clonorchis sinensis, the chinese liver fluke, is the causative agent of clonorchiasis as well as liver and biliary diseases. the excretory-secretory products (esps) of the parasites play important roles in host-parasite interactions. in this study, we have investigated the proteome of esps obtained from c. sinensis adult worms. although the full genome database of c. sinensis is not yet available, we have successfully identified 62 protein spots using 2-de-based mass analysis and est database of ... | 2009 | 19526557 |
| emerging food-borne parasites. | parasitic food-borne diseases are generally underrecognised, however they are becoming more common. globalization of the food supply, increased international travel, increase of the population of highly susceptible persons, change in culinary habits, but also improved diagnostic tools and communication are some factors associated with the increased diagnosis of food-borne parasitic diseases worldwide. this paper reviews the most important emerging food-borne parasites, with emphasis on transmiss ... | 2009 | 19559535 |
| application of signaling protein 14-3-3 and 26 kda glutathione-s-transferase to serological diagnosis of schistosomiasis japonica. | schistosomiasis japonica is currently one of the most serious parasitic diseases and over 670000 people are infected in china by the end of 2006. in order to establish an effective diagnostic method, the gene coding for sj14-3-3 and sj26kda gst were cloned and expressed separately in escherichia coli as fusion protein with his-tag. the rsj14-3-3 and 26kda rsjgst were combinedly used as antigens for enzyme-linked immunosorbent assays (elisa) to diagnose acute and chronic s. japonica. our results ... | 2009 | 19560432 |
| combination chemotherapy against clonorchis sinensis: experiments with artemether, artesunate, oz78, praziquantel, and tribendimidine in a rat model. | caused by the chinese liver fluke clonorchis sinensis, clonorchiasis is of growing public health importance. treatment and control of the disease rely on a single drug, praziquantel, and little information regarding combination chemotherapy is available. here, we evaluated the in vivo efficacy of praziquantel combined with artemether, artesunate, oz78, and tribendimidine, as well as an artesunate-tribendimidine combination against c. sinensis, in a rat model. data from previous experiments were ... | 2009 | 19581454 |
| food-borne trematodiases. | an estimated 750 million people are at risk of infections with food-borne trematodes, which comprise liver flukes (clonorchis sinensis, fasciola gigantica, fasciola hepatica, opisthorchis felineus, and opisthorchis viverrini), lung flukes (paragonimus spp.), and intestinal flukes (e.g., echinostoma spp., fasciolopsis buski, and the heterophyids). food-borne trematodiases pose a significant public health and economic problem, yet these diseases are often neglected. in this review, we summarize th ... | 2009 | 19597009 |
| the in vitro and in vivo effect of tribendimidine and its metabolites against clonorchis sinensis. | the purpose of the study was to understand the in vitro and in vivo effect of tribendimidine (tbd) and its metabolites of p-(1-dimethylamino ethylimino)aniline (aminoamidine, deacylated amidantel, bay d 9216, dadt), acetylated dadt (adadt), terephthalaldehyde (tpal), and terephthalic acid (tpac) against adult clonorchis sinensis. in in vitro test, the adults of c. sinensis were placed to each of the 24 wells of a falcon plate and maintained in hanks' balanced salt solution-20% calf serum. beside ... | 2009 | 19655171 |
| prevalence and risks for fishborne zoonotic trematode infections in domestic animals in a highly endemic area of north vietnam. | fishborne zoonotic trematodes (fzt) are endemic in humans and cultured fish in vietnam but little is known about fzt in domestic animals. a study was designed to determine fzt prevalence and species diversity, and risk factors for infection, in dogs, cats and pigs. faecal samples from 186 dogs, 94 cats and 168 pigs belonging to 132 households in nghia hung district, nam dinh province, were examined for small trematode eggs; those were trematode eggs with length less than 50 microm. prevalence of ... | 2009 | 19660428 |
| detection of clonorchis sinensis in stool samples using real-time pcr. | human clonorchiasis, caused by infection with the trematode clonorchis sinensis, is a common health problem in east asia. in an attempt to develop a new, sensitive method for the diagnosis of the disease, the use of a real-time pcr (targeting the internal-transcribed-spacer-2 sequence of the parasite) to detect c. sinensis-specific dna in faecal samples has recently been evaluated. the pcr-based assay, which included an internal control to detect any inhibition of the amplification by faecal con ... | 2009 | 19695156 |
| fishborne trematode metacercariae in freshwater fish from guangxi zhuang autonomous region, china. | a survey was performed to investigate the infection status of fishborne trematode (fbt) metacercariae in freshwater fish from guangxi zhuang autonomous region, china. a total of 307 freshwater fish of 31 species were collected from 5 administrative regions of guangxi zhuang autonomous region. they were examined by artificial digestion method from july 2003 to august 2004. no metacercariae were detected in fish from fusui-xian. in fish from mashan-xian and a market in nanning, 3 species of metace ... | 2009 | 19724698 |
| oriental liver fluke infestation presenting more than 50 years after immigration. | | 2009 | 19736582 |
| experimental model in rats for study on transmission dynamics and evaluation of clonorchis sinensis infection immunologically, morphologically, and pathologically. | this study aims to gain a better insight into the transmission patterns and immunologic profile of clonorchis sinensis infection and make a headway on the pathogenesis regarding cholangiocarcinoma and hepatic lesions. experimental models orally infected by c . sinensis metacercariae were constructed in rats. immunological assays were performed to measure serum level of iga, ige, igg1, igg2a, ifn-gamma, and il-4. infection parameters were assessed by worm recovery rate, eggs per gram faece and wo ... | 2009 | 19756744 |
| cloning, characterization, and expression of a novel secretory lipase-like gene from clonorchis sinensis. | a secretory lipase-like gene was isolated from total cdna of adult clonorchis sinensis. the gene has an open reading frame of 1,218 bp long and encodes for a protein of 406 amino acids including a putative signal peptide of 20 amino acids. the deduced amino acid sequence including signal peptide has 42-45% identity with lipase of other species and two typical enzymic active sites that contain consensus sequence (gly-x-ser-x-gly) of lipase. the cdna encoding this protein was subcloned into pet-28 ... | 2009 | 19756752 |
| differential ct features of intraductal biliary metastasis and double primary intraductal polypoid cholangiocarcinoma in patients with a history of extrabiliary malignancy. | the purpose of this study was to discern clinical and imaging features for differentiating intraductal metastasis from double primary intraductal cholangiocarcinoma in patients with a history of extrabiliary malignant disease. | 2009 | 19770330 |
| a novel nuclear marker, pm-int9, for phylogenetic studies of opisthorchis felineus, opisthorchis viverrini, and clonorchis sinensis (opisthorchiidae, trematoda). | opisthorchis felineus, o. viverrini, and clonorchis sinensis, the trematodes of the family opisthorchiidae, are important human parasites. two previous studies (kang et al. parasitol int 57:191-197, 2008; katokhin et al. dokl biochem biophys 421:214-217, 2008) have provided evidence using ribosomal and mitochondrial sequences that o. viverrini, o. felineus, and c. sinensis are closely related. we developed a novel nuclear marker, pm-int9, which included the ninth intron of the paramyosin gene an ... | 2009 | 19777262 |
| proteomic analysis of differentially expressed proteins in human cholangiocarcinoma cells treated with clonorchis sinensis excretory-secretory products. | severe clonorchis sinensis infection is a significant risk factor for malignant changes in bile ducts and surrounding liver tissues occurring as a result of direct contact with c. sinensis worms and their excretory-secretory products (esp). however, the intrinsic molecular mechanisms involved in these processes remain obscure. to determine the effects of c. sinensis infection on protein expression in host bile duct epithelium, we examined proteomic profile changes in the human cholangiocarcinoma ... | 2009 | 19798681 |
| efficacy of artesunate and artemether against clonorchis sinensis in rabbits. | artesunate and artemether display promising clonorchicidal properties in rats. in this study, we assessed the efficacy of artesunate and artemether against clonorchis sinensis in rabbits. rabbits were each fed with 300 c. sinensis metacercariae. at day 28 postinfection, the rabbits were administered oral artesunate at doses of 7.5-120 mg/kg and oral artemether of 15-120 mg/kg. two groups of rabbits were treated with single oral praziquantel at 75 and 150 mg/kg. untreated rabbits served as contro ... | 2009 | 19802632 |
| parasites as aetiological agents in chronic diseases. epidemiological associations and potential mechanisms of pathogenesis. | the aetiological role of parasitic infection has been well established, through epidemiological studies, for many chronic diseases prevalent in the tropics. examples include schistosoma mansoni infection leading to portal hypertension, schistosoma haematobium infection leading to obstructive uropathy and squamous cell carcinoma of the bladder, clonorchis sinensis leading to cholangiocarcinoma, and taenia solium infection leading to epilepsy. our understanding of the pathogenesis of these associa ... | 2009 | 19825104 |
| helminths and malignancy. | it has been estimated that chronic infections with viruses, bacteria and parasites contribute to 17.8% of the global burden of cancer, although only a relatively small proportion of the infection-related cancers can be attributed to helminth infections. these are important because of the high number of people who are exposed or infected worldwide. carcinogenesis associated with helminth infections is a complex process, which may involve several different mechanisms, but chronic inflammation is a ... | 2009 | 19825108 |
| [therapeutic effect of tribendimidine, artesunate and praziquantel administered to hamsters infected with clonorchis sinensis]. | to observe the effect of tribendimidine, artesunate and praziquantel in treatment of hamsters (mesocricetus auratus) infected with clonorchis sinensis. | 2009 | 19852361 |
| [tissular and subcellular localization of the atp synthase b subunit in clonorchis sinensis]. | to illustrate the distribution of atp synthase b subunit in the tissue of clonorchis sinensis adult and its subcellular mimical localization in hela cells. | 2009 | 19856493 |
| [heavy infection of clonorchis sinensis with complication of cholelithiasis]. | | 2009 | 19856495 |
| [experimental establishment of life cycle of clonorchis sinensis]. | to establish and maintain the life cycle of clonorchis sinensis in laboratory. | 2009 | 19856506 |
| molecular cloning and characterization of a novel serpin gene of clonorchis sinensis, highly expressed in the stage of metacercaria. | the serpins are a superfamily of proteins (350-500 amino acids in size) that fold into a conserved structure. from about 3,475 unigenes of clonorchis sinensis metacercaria, a novel gene-encoding serpin was identified and characterized. the opening reading frame is 1,149 bp encoding 382 amino acids. the deduced amino acid sequence shows high identity to previously reported serpins from c. sinensis and other helminthic parasites. a typical serpin signature was found by motif search. the recombinan ... | 2009 | 19859738 |
| fish-borne zoonotic trematode metacercariae in the republic of korea. | the prevalence of fish-borne trematodes (fbt), including clonorchis sinensis, is still high in riverside areas of the republic of korea. the author reviewed the detection and identification methods, differential keys, fish intermediate hosts, and morphological characteristics of fbt metacercariae. fbt metacercariae found in freshwater fish are classified mainly into 4 families, i.e., opisthorchiidae, heterophyidae, echinostomatidae, and clinostomidae. the metacercariae of c. sinensis, found in 4 ... | 2009 | 19885326 |
| review of zoonotic parasites in medical and veterinary fields in the republic of korea. | zoonotic parasites are animal parasites that can infect humans. the major zoonotic protozoa in the republic of korea are babesia bovis, chilomastix mesnili, cryptosporidium parvum, endolimax nana, entamoeba coli, entamoeba hitolytica, giardia lamblia, iodamoeba bütschlii, pneumocystis carinii, sarcocystis cruzi, and toxoplasma gondii. the major zoonotic helminths in korea include trematodes, cestodes, and nematodes. trematodes are clonorchis sinensis, echinostoma hortense, echinostoma spp., fasc ... | 2009 | 19885329 |
| functional genes and proteins of clonorchis sinensis. | during the past several decades, researches on parasite genetics have progressed from biochemical and serodiagnostic studies to protein chemistry, molecular biology, and functional gene studies. nowadays, bioinformatics, genomics, and proteomics approaches are being applied by korean parasitology researchers. as for clonorchis sinensis, investigations have been carried out to identify its functional genes using forward and reverse genetic approaches and to characterize the biochemical and biolog ... | 2009 | 19885336 |
| gene expression profiling in mouse liver infected with clonorchis sinensis metacercariae. | clonorchis sinensis, the parasite that causes clonorchiasis, is endemic in many asian countries, and infection with the organism drives changes in the liver tissues of the host. however, information regarding the molecular events in clonorchiasis remains limited, and little is currently known about host-pathogen interactions in clonorchiasis. in this study, we assessed the gene expression profiles in mice livers via dna microarray analysis 1, 2, 4, and 6 weeks after induced metacercariae infecti ... | 2009 | 19902254 |
| the complete mitochondrial genomes of the liver flukes opisthorchis felineus and clonorchis sinensis (trematoda). | the complete mitochondrial genomes of the parasitic trematodes opisthorchis felineus and clonorchis sinensis (family opisthorchiidae) were fully sequenced in order to develop markers for dna diagnostics of the liver flukes infection, molecular ecology, population and phylogenetic studies. the complete sequences of mitochondrial genomes of these species comprise 14,277 and 13,875bp, respectively, and are thus the shortest trematode mitochondrial genomes sequenced to date. the gene content and arr ... | 2010 | 19906359 |
| a family of cathepsin f cysteine proteases of clonorchis sinensis is the major secreted proteins that are expressed in the intestine of the parasite. | cysteine proteases of helminth parasites play essential roles in parasite physiology as well as in a variety of important pathobiological processes. in this study, we identified a multigene family of cathepsin f cysteine proteases in clonorchis sinensis (cscfs). we identified a total of 12 cscf genes through cdna cloning using degenerate pcr primers followed by race. sequence and phylogenetic analysis of the genes suggested they belonged to the cathepsin f-like enzyme family and further clustere ... | 2010 | 19932715 |
| oriental cholangiohepatitis (clonorchiasis infestation) caused by clonorchis sinensis. | background: oriental cholangiohepatitis (clonorchis infestation) is caused by clonorchis sinensis, a liver fluke endemic to china. objective: to discuss the presentation of clonorchiasis and diagnosis of this condition in the emergency department (ed). case report: this is a case report of a chinese woman who recently immigrated to the united states and was evaluated in a tertiary care urban ed. the patient presented with complaints of abdominal pain and was found on imaging to have clonorchiasi ... | 2009 | 19959317 |
| molecular cloning and characterization of a paramyosin from clonorchis sinensis. | paramyosin is a myofibrillar protein present in helminth parasites and plays multifunctional roles in host-parasite interactions. in this study, we identified the gene encoding paramyosin of clonorchis sinensis (cspmy) and characterized biochemical and immunological properties of its recombinant protein. cspmy showed a high level of sequence identity with paramyosin from other helminth parasites. recombinant cspmy (rcspmy) expressed in bacteria had an approximate molecular weight of 100 kda and ... | 2009 | 19967083 |
| elucidating the transcriptome of fasciola hepatica - a key to fundamental and biotechnological discoveries for a neglected parasite. | liver flukes of animals are parasitic flatworms (platyhelminthes: digenea) of major socioeconomic importance in many countries. key representatives, such as fasciola hepatica and f. gigantica, cause "liver fluke disease" (= fascioliasis), which is of major animal health significance worldwide. in particular, f. hepatica is a leading cause of production losses to the livestock (mainly sheep and cattle) and meat industries due to clinical disease, reduced weight gain and milk production, and death ... | 2010 | 20006979 |
| [intrahepatic cholangiocarcinoma associated with clonorchis sinensis infection]. | | 2009 | 20037272 |
| parasitic causes of pancreatic and biliary tract disease: a growing concern in a highly mobile population. | a leading cause of biliary tract disease and pancreatitis worldwide is parasitic disease. in the united states, increased global travel and the aids epidemic has led to a rise in the frequency of parasitic disease. biliary disease and pancreatic disease secondary to parasitic infestation is relatively new in this country, with the first case being described in 1977. these diseases are no longer the exclusive realm of infectious disease specialists and require general practitioners and gastroente ... | 1994 | 20058432 |
| epidemiology of cholangiocarcinoma: an update focusing on risk factors. | cholangiocarcinoma is relatively rare, but high incidence rates have been reported in eastern asia, especially in thailand. the etiology of this cancer of the bile ducts appears to be mostly due to specific infectious agents. in 2009, infections with the liver flukes, clonorchis sinensis or opistorchis viverrini, were both classified as carcinogenic to humans by the international agency for research on cancer for cholangiocarcinoma. in addition, a possible association between chronic infection w ... | 2010 | 20085587 |
| an effective sequence characterized amplified region-pcr method derived from restriction site-amplified polymorphism for the identification of female schistosoma japonicum of zoonotic significance. | in the present study, restriction site-amplified polymorphism (rsap) markers were used to examine the genetic variability of schistosoma japonicum isolates from different endemic provinces in mainland china. of the 45 pairs of primers screened, 10 rsap markers showed a clear banding pattern with good resolution; however, only six exhibited a polymorphism among different isolates. among six rsap markers, one pair of primers (r8+r10) was able to differentiate male and female parasites, and amplifi ... | 2010 | 20108264 |
| cholangiocarcinoma: descriptive epidemiology and risk factors. | in theory, the term of cholangiocarcinoma is reserved for the tumours originating from the intrahepatic bile ducts. the problems of classification of the most frequent hilar tumours and the absence of histopathological confirmation in a large percentage of cases in cancer registries from many countries show the difficulty of establishing the specific epidemiologic behaviour of intrahepatic cholangiocarcinoma (icc). there are clearly two types of icc: the first one is the consequence of the recur ... | 2010 | 20202771 |
| sensitive and rapid detection of clonorchis sinensis infection in fish by loop-mediated isothermal amplification (lamp). | the fish-borne clonorchiasis caused by the oriental liver fluke clonorchis sinensis is endemic in a number of countries with over 35 million people being infected globally. rapid and accurate detection of c. sinensis in its intermediate host fish is important for the control and prevention of clonorchiasis in areas where the disease is endemic. in the present study, we established a loop-mediated isothermal amplification (lamp) approach for the sensitive and rapid detection of c. sinensis metace ... | 2010 | 20232082 |
| [epidemiological survey on clonorchiasis sinensis in an endemic area of south hunan province]. | to make an epidemiological investigation on clonorchiasis sinensis and relevant factors in the south of hunan province. | 2009 | 20232625 |
| transcriptional induction of minichromosome maintenance protein 7 (mcm7) in human cholangiocarcinoma cells treated with clonorchis sinensis excretory-secretory products. | clonorchiasis is an infection associated with bile duct malignancy and subsequent development of cholangiocarcinoma. this disease is mainly caused by clonorchis sinensis worms and their excretory-secretory products (esp). however, the precise molecular mechanisms of carcinogenesis remain to be determined. previously, we established differential gene expression profiles from microarrays containing 23,920 human genes of known function in a human cholangiocarcinoma cell line, hucct1, treated with e ... | 2010 | 20236609 |
| a histidine substitution confers metal binding affinity to a schistosoma japonicum glutathione s-transferase. | glutathione s-transferases (gsts) are multifunctional enzymes that are used as fusion tags on recombinant proteins in mammalian and escherichia coli expression systems. we recently found that the schistosoma japonicum gst (sjgst) displays weak ni(2+) ion binding affinity. glu26 and his79 were assumed to be its ni(2+) binding sites based on the structure of the 26-kda clonorchis sinensis gst. to enhance sjgst ni(2+) binding affinity, glu26 was mutated to his. sjgst-e26h was expressed and purified ... | 2010 | 20347989 |
| food-borne trematode infections of humans in the united states of america. | this review examines the literature on imported (allochthonous) and local (autochthonous) cases of food-borne trematode (fbt) infections in the united states of america (usa) from 1890 to 2009. most of the literature is concerned with imported cases of the opisthorchiids clonorchis sinensis and opisthorchis viverrini. these flukes cause serious pathology in the liver and biliary system of humans. chronic cases may induce liver (hepatocarcinoma) and bile duct (cholangiocarcinoma) cancers in human ... | 2010 | 20352454 |
| fish-borne zoonotic trematodes in cultured and wild-caught freshwater fish from the red river delta, vietnam. | nam dinh province in red river delta, vietnam, is an endemic area for the human liver fluke clonorchis sinensis, but little is known about its occurrence in the fish intermediate host in this region. a cross-sectional study was carried out to identify fish-borne zoonotic trematodes (fzt) species diversity and to estimate the prevalence and infection densities of fzt in three major types of fish: cultured species and self-recruiting species from family ponds and wild-caught species from irrigatio ... | 2010 | 20370427 |
| effect of artemether, artesunate, oz78, praziquantel, and tribendimidine alone or in combination chemotherapy on the tegument of clonorchis sinensis. | we investigated the morphological effects of half-strength treatments with praziquantel, artemether, artesunate, oz78 and tribendimidine as well as combinations of praziquantel with artemether, artesunate, oz78 and tribendimidine and an artesunate-tribendimidine combination in rats harboring adult clonorchis sinensis. rats were infected with c. sinensis, dosed orally with single agents or combination treatments and flukes recovered at 3 or 5 days post-treatment. the number of flukes was counted, ... | 2010 | 20416396 |
| performance of an enzyme-linked immunosorbent assay for detection of clonorchis sinensis infestation in high- and low-risk groups. | clonorchis sinensis is still a common parasite in south korea. an enzyme-linked immunosorbent assay (elisa) was developed to replace the usual intradermal test, and its performance in an area of endemicity was evaluated. from 2004 to 2006, 182 adults were included. the patient group consisted of 51 adults; 43 patients showed fluke eggs by microscopy, and 8 had their disease diagnosed clinically. the negative-control group included 131 adults: 98 at low risk and 33 at high risk of infestation. bo ... | 2010 | 20421441 |
| distribution of freshwater snails in family-based vac ponds and associated waterbodies with special reference to intermediate hosts of fish-borne zoonotic trematodes in nam dinh province, vietnam. | fish-borne zoonotic trematodes, such as clonorchis sinensis, heterophyids and others, constitute a public health concern in parts of northern vietnam and infections with these trematodes are often thought to be linked to fish culture. one common fish culture system is the integrated fish-livestock (vac) ponds where individual households have 1 or more ponds. fish fry, mainly of various carp species, produced in hatcheries, not necessarily local, are introduced into nursery ponds and after approx ... | 2010 | 20457118 |
| chronic bacterial and parasitic infections and cancer: a review. | a relatively underestimated facet of infectious diseases is the association of chronic bacterial and parasitic infections with cancer development. therefore, we sought to evaluate the evidence regarding the association of such infections with the development of malignancy, excluding the overwhelming evidence of the association of helicobacter pylori and cancer. | 2010 | 20539059 |
| epidemiological investigation of clonorchis sinensis infection in freshwater fishes in the pearl river delta. | pearl river delta region is a high clonorchiasis-endemic area in china. however, no complete epidemiological data exist regarding its infection in freshwater fishes, an important epidemic factor for clonorchis sinensis. the present study collected freshwater fishes and shrimps from 32 sites of nine cities in the pearl river delta, and the encysted metacercariae of c. sinensis were detected by digesting these specimens with artificial gastric juice. the mean infection rate of freshwater fishes wa ... | 2010 | 20549239 |
| unlocking the transcriptomes of two carcinogenic parasites, clonorchis sinensis and opisthorchis viverrini. | the two parasitic trematodes, clonorchis sinensis and opisthorchis viverrini, have a major impact on the health of tens of millions of humans throughout asia. the greatest impact is through the malignant cancer ( = cholangiocarcinoma) that these parasites induce in chronically infected people. therefore, both c. sinensis and o. viverrini have been classified by the world health organization (who) as group 1 carcinogens. despite their impact, little is known about these parasites and their interp ... | 2010 | 20582164 |
| epidemiological survey on the infection of intestinal flukes in residents of muan-gun, jeollanam-do, the republic of korea. | infection status of intestinal flukes was investigated in residents of muan-gun, jeollanam-do, the republic of korea. total 1,257 fecal samples of residents were examined by formalin-ether sedimentation technique and kato-katz thick smear method. helminth eggs were detected from 95 (7.6%) residents, and eggs of heterophyid flukes and clonorchis sinensis were found from 62 (4.9%) and 40 (3.2%) cases, respectively. the larger heterophyid eggs, somewhat dark-brown in color and 37.7 x 21.5 microm in ... | 2010 | 20585529 |
| a case of probable mixed-infection with clonorchis sinensis and fasciola sp.: ct and parasitological findings. | we report here a human case probably mixed-infected with clonorchis sinensis and fasciola sp. who was diagnosed by computed tomography (ct) scan, serological findings, and/or fecal examination. the patient was a 43-year-old korean female and was admitted to kyung hee university hospital with the complaints of fever and abdominal pain. on admission, marked eosinophilia was noted in her peripheral blood. ct scan showed specific lesions for clonorchiasis and fascioliasis in the liver, along with le ... | 2010 | 20585533 |
| descriptive epidemiology of cholangiocarcinoma and clonorchiasis in korea. | in 2009, infection with the liver fluke clonorchis sinensis (c. sinensis) was classified as "carcinogenic to humans" (group 1) based on its involvement in the etiology of cholangiocarcinoma by the international agency for research on cancer. however, little is known about the descriptive epidemiology of cholangiocarcinoma in korea. we examined incidence trends of intrahepatic and extrahepatic cholangiocarcinomas, using data from the korea national cancer incidence database for 1999-2005. the pre ... | 2010 | 20592891 |
| identification and characterization of a serine protease inhibitor of clonorchis sinensis. | a gene encoding a serine protease inhibitor of clonorchis sinensis (csserpin) was identified and characterized. csserpin contained an open reading frame of 1158bp that encoded 385 amino acid residues. sequence analysis of the primary structure of csserpin revealed that it had essential structural motifs including a reactive central loop (rcl), which well conserved in the serine protease inhibitor (serpin) superfamily. csserpin was classified as a member of the ovalbumin-type serpin family on the ... | 2010 | 20603096 |
| [dna diagnosis of mixed invasions of opisthorchis felineus and metorchis bilis by polymerase chain reaction]. | opisthorchiasis is one of the significant naturofocal diseases in russia. the diagnosis of opisthorchiasis may mask some diseases, such as opisthorchiasis, metorchiasis, and clonorchiasis - biohelminthoses that are induced by various representatives of the family opisthorchiidae-opisthorchis felineus/o.viverrini, metorchis bilis, and clonorchis sinensis, respectively. coproovoscopy and serologic methods fail to accurately define the species-specific affiliation; in this connection the identifica ... | 2010 | 20614520 |
| food-borne trematodiases in southeast asia epidemiology, pathology, clinical manifestation and control. | the food-borne trematodiases are an important group of neglected tropical diseases (ntds). over 40 million people are infected with food-borne trematodes and 750 million (>10% of the world's population) are at risk of these ntds. here, we review the life cycles, epidemiology, clinical manifestations, pathology and pathogenesis, diagnosis, treatment, and prevention and control of the major food-borne trematodiases in southeast asia. we focus particularly on opisthorchiasis caused by opisthorchis ... | 2010 | 20624536 |
| towards improved diagnosis of zoonotic trematode infections in southeast asia. | humans in southeast asia are at risk for at least 70 species of food-borne and water-borne trematodes, including blood flukes, intestinal flukes, liver flukes and lung flukes, which are shared with a great variety of animals. co-infection with several other zoonotic trematodes is pervasive, and hence differential diagnosis represents a major challenge. many zoonotic trematodes are commonly overlooked, leading to unreliable prevalence data, underappreciation of their veterinary and public health ... | 2010 | 20627143 |
| farm-level risk factors for fish-borne zoonotic trematode infection in integrated small-scale fish farms in northern vietnam. | northern vietnam is an endemic region for fish-borne zoonotic trematodes (fzt), including liver and intestinal flukes. humans acquire the fzt infection by eating raw or inadequately cooked fish. the production of fzt-free fish in aquaculture is a key component in establishing a sustainable program to prevent and control the fzt transmission to humans. interventions in aquaculture should be based on knowledge of the main risk factors associated with fzt transmission. | 2010 | 20644617 |
| [infection status of freshwater fishes with metacercariae of clonorchis sinensis in liujiang river of guangxi]. | from march 2008 to march 2009, fishes were caught from the rivers in sanjiang, rongan, rongshui, liucheng, liuzhou and xiangzhou counties, and their metacercarial infections were examined by the muscle compression and digestion techniques. a total of 16,204 freshwater fishes of 35 species were collected. c. sinensis metacercariae were found in 32 species of fishes with an overall infection rate of 10.5% and a mean infection intensity of 4.6 metacercariae per gram. the highest prevalence (21.5%) ... | 2010 | 20666325 |
| helminths in human carcinogenesis. | this review examines the salient literature on selected helminths involved in carcinogenicity in humans and updates information in an earlier review on cancer and helminths by mayer and fried (2007, advances in parasitology 65, 239-296). the earlier review was concerned with various helminths, i.e., trematodes, cestodes, and nematodes, that are definitely implicated as being carcinogenic. this review examines only those helminths, all of which turn out to be trematodes, that are definitely impli ... | 2011 | 20667649 |
| progress on the transcriptomics of carcinogenic liver flukes of humans--unique biological and biotechnological prospects. | liver flukes, such as clonorchis sinensis and opisthorchis viverrini, are food-borne parasites that have a major impact on the health of humans and animals, particularly in asia. however, the impact of c. sinensis and o. viverrini, in particular, is exacerbated in that these parasites can induce a malignant, untreatable cancer (cholangiocarcinoma, cca) in chronically infected people. as a result, these flukes are classified as group 1 carcinogens. despite their substantial socio-economic importa ... | 2010 | 20673796 |
| effectiveness of mefloquine against clonorchis sinensis in rats and paragonimus westermani in dogs. | the aim of the study is to explore the effect of mefloquine against clonorchis sinensis and paragonimus westermani. for anti-c. sinensis study, a total of 71 rats were divided into four batches for oral infection of each rat with 50 c. sinensis metacercariae. five to 7 weeks post-infection, groups of rats were treated orally with mefloquine at single doses or multiple daily doses while infected, but untreated rats served as control. all treated rats were euthanized 2 weeks post-treatment for ass ... | 2010 | 20680332 |
| molecular characterization and expression of the mynd-zf gene from clonorchis sinensis. | the mynd-type zinc finger protein (mynd-zf) is a large group of proteins containing the mynd domain which play an important role in protein-protein interactions. a cdna clone encoding a novel mynd-zf was isolated and identified from a clonorchis sinensis (c. sinensis) adult cdna library. the open reading frame of this novel cdna sequence contains 1,440 base pairs with a putative protein of 479 amino acids showing a high homology with the mynd-zf identified from other species. recombinant csmynd- ... | 2010 | 20680338 |
| update on the diagnosis and treatment of food-borne trematode infections. | more than 40 million people are affected by food-borne trematode infections. diagnosis is unsatisfactory and there are only two drugs available for treatment and control: praziquantel and triclabendazole. this review provides an update on recent developments in the diagnosis and treatment of food-borne trematodiases. | 2010 | 20683264 |
| opisthorchiidosis - a review. | the discovery history of opisthorchiid flukes dates back more than 200 years. the life cycle of these liver flukes includes prosobranch snails as the first and a big variety of freshwater fish as the second intermediate hosts. opisthorchis felineus, o. viverrini and clonorchis sinensis are species of major medical importance, while sporadic cases of human infection with other representatives of this family are also documented in the literature. there are estimations that 17 million people are in ... | 2010 | 20701569 |
| the digenea parasite opisthorchis felineus: a target for the discovery and development of novel drugs. | opisthorchosis is a helminthiasis affecting mainly the hepatobiliary system and pancreas; its most dramatic complication is malignization of the organs infected by the parasites. the causative agents of opisthorchosis are two species of liver flukes, the trematodes belonging to the family opisthorchiidae--opisthorchis felineus and o. viverrini. the chinese liver fluke, clonorchis sinensis, also member of the family opisthorchiidae, causes clonorchosis, a disease very close in symptomatology. acc ... | 2010 | 20701571 |
| [treatment of rats infected with clonorchis sinensis using clinical administration regimens of tribendimidine, praziquantel and artesunate]. | to evaluate the efficacy in treatment of clonorchis sinensis-infected rats using the administration regimens of tribendimidine, artesunate and praziquantel applied in clinical treatment of clonorchiasis. | 2010 | 20806495 |
| [protective immunity of cs-rho gtpase recombinant protein against clonorchis sinensis infection]. | to study the protective immunity induced by recombinant vaccination of cs-rho gtpase of clonorchis sinensis (cs). | 2010 | 20806498 |
| seroprevalence of tissue invading parasitic infections diagnosed by elisa in korea. | seroprevalence of the igg antibodies for clonorchis sinensis, paragonimus westermani, taenia solium metacestode (cysticercus), and spirometra erinacei plerocercoid (sparganum) was measured using enzyme-linked immunosorbent assay (elisa) in sera of patients in korea from 1993 to 2006. a total of 74,448 specimens referred nationwide from 121 hospitals revealed an igg positive rate of 7.6% for the 4 parasites. the igg positive rate (18.7%) for the 4 parasites in 1993 decreased gradually to 6.6% in ... | 2010 | 20808668 |
| cloning and expression of 21.1-kda tegumental protein of clonorchis sinensis and human antibody response to it as a trematode-nematode pan-specific serodiagnosis antigen. | a complete cdna encoding a 21.1-kda tegumental protein (cstp21.1) was recognized from clonorchis sinensis adult full-length cdna plasmid library by bioinformatics analysis. recombinant cstp21.1 was highly expressed in escherichia coli, purified by affinity chromatography, and identified by western blotting. immunohistochemistry demonstrated that cstp21.1 is localized in the tegument of the adult worm. the rcstp21.1-specific igg1, igg2, and igg4 subclasses could be detected in the sera of clonorc ... | 2010 | 20865432 |
| identification and characterization of micrornas in clonorchis sinensis of human health significance. | clonorchis sinensis is a zoonotic parasite causing clonorchiasis-associated human disease such as biliary calculi, cholecystitis, liver cirrhosis, and it is currently classified as carcinogenic to humans for cholangiocarcinoma. micrornas (mirnas) are non-coding, regulating small rna molecules which are essential for the complex life cycles of parasites and are involved in parasitic infections. to identify and characterize mirnas expressed in adult c. sinensis residing chronically in the biliary ... | 2010 | 20920166 |
| rapid identification and differentiation of fasciola hepatica and fasciola gigantica by a loop-mediated isothermal amplification (lamp) assay. | the present study developed and validated a species-specific loop-mediated isothermal amplification (lamp) assay for the rapid detection and discrimination of fasciola hepatica and fasciola gigantica. the lamp assay is inexpensive, easy to perform and shows rapid reaction, wherein the amplification can be obtained in 45 min under isothermal conditions of 61 °c or 62 °c by employing a set of four species-specific primer mixtures and results can be checked through naked-eye visualization. the opti ... | 2010 | 20933335 |
| cholangiocarcinoma: new insights into disease pathogenesis and biology. | cholangiocarcinomas are rare malignant tumors whose incidence is increasing worldwide. risk factors for this malignancy include both infectious and non-infectious diseases characterized by chronic inflammation of the bile duct epithelia. diagnosis of these cancers remains difficult because of the lack of sensitive diagnostic tests. the prognosis is poor probably because of the lack of effective treatments for unresectable cancer. | 2010 | 20937455 |
| [a case of clonorchiasis presenting as common bile duct mass]. | | 2010 | 20962555 |
| [not available]. | | 1946 | 20982372 |
| infection, stem cells and cancer signals. | the association of cancer with preceding parasitic infections has been observed for over 200 years. some such cancers arise from infection of tissue stem cells by viruses with insertion of viral oncogenes into the host dna (mouse polyoma virus, mouse mammary tumor virus). in other cases the virus does not insert its dna into the host cells, but rather commandeers the metabolism of the infected cells, so that the cells continue to proliferate and do not differentiate (human papilloma virus and ce ... | 2011 | 21044009 |
| monoclonal antibodies against excretory/secretory antigens of angiostrongylus cantonensis. | in the present study, four murine monoclonal antibodies (mabs) were generated against the excretory/secretory (es) products of angiostrongylus cantonensis adult worms; two represented igg1 and two represented igm mabs, and they were designated 12d5, 15f8, 21b7 and 14g10, respectively. immunoblotting revealed that all of the mabs predominantly recognized a 98 kda antigen in the es products of a. cantonensis adult worms, and no cross reactions were found with the whole worm antigens of some other ... | 2010 | 21050048 |
| molecular cloning and characterization of a novel ras-related protein (rap2) from clonorchis sinensis. | ras are key components of diverse signal transduction pathways and play important roles in growth and development. to know about growth regulation in clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cdna library. the open reading frame contains 561 bp encoding 186 amino acids. the hypothetical amino acid sequence shared high identities with rap2 proteins from schistosoma japonicum and homo sapiens. conserved domains of small guan ... | 2011 | 21061020 |
| sensitive and rapid detection of paragonimus westermani infection in humans and animals by loop-mediated isothermal amplification (lamp). | in the present study, a loop-mediated isothermal amplification (lamp) assay was developed and validated for the detection of paragonimus westermani adults, metacercariae, and eggs in human and animal samples. the lamp amplification can be finished in 45 min under isothermal condition at 60°c by employing a set of four species-specific primer mixtures and the results can be checked by naked-eye visualization. no amplification products were detected with deoxyribunucleic acid (dna) of related trem ... | 2010 | 21107864 |
| not just a fluke: expanding the organ supply. | | 2010 | 21117192 |
| feasibility of using a liver infected with clonorchis sinensis for liver transplantation: fourteen cases. | use of livers infected with clonorchis sinensis as donor organs for transplantation is controversial because of the potential associated risks. the low availability of donor livers at tianjin first center hospital since 2003 prompted us to undertake cadaveric liver transplantation in 14 patients using donor livers infected with c. sinensis. none of the donors had been diagnosed with liver fluke infection before organ procurement, and in none of them was there laboratory evidence of abnormal live ... | 2010 | 21117256 |
| multiple recombinant antigens of clonorchis sinensis for serodiagnosis of human clonorchiasis. | antigenic proteins from clonorchis sinensis have been previously purified and evaluated for their antigenicity to enable the serodiagnosis of clonorchiasis. though they were of high specificity, molecularly defined proteins were reported to be less sensitive as single antigens than crude antigen. to resolve this issue, 11 clones were selected by immunoscreening an adult c. sinensis cdna library using infected human sera. mixed antigens were prepared using recombinant proteins of positive clones ... | 2010 | 21125293 |
| comparative effect of mebendazole, albendazole, tribendimidine, and praziquantel in treatment of rats infected with clonorchis sinensis. | the aim of the study is to understand the anti-clonorchis sinensis properties of mebendazole and albendazole, and compare to praziquantel and tribendimidine. two hundred and thirty rats were divided into five batches for experimental treatment. in four batches, each rat was infected orally with 50 or 100 c. sinensis metacercariae. twenty-eight to 46 days post-infection, groups of rats were treated orally with single doses of mebendazole, albendazole, praziquantel, tribendimidine, or multiple dai ... | 2010 | 21136080 |
| [research progress on the relationship between three kinds of liver fluke infections and cholangiocarcinoma]. | liver fluke disease caused by clonorchis sinensis, opisthorchis viverrini and o. felineus is a food-borne zoonotic parasitic disease and widely prevalent in asia. the definitive hosts including human beings get the infection by ingestion of raw or undercooked fish or shrimp infested with metacercariae. long-term or severe infection of the flukes can lead to dysfunction of the liver, such as cholelithiasis, cholecystitis, and so on. researches indicated that there is an etiology relation between ... | 2010 | 21137319 |
| obstructive jaundice caused by clonorchiasis-associated duodenal papillitis: a case report. | we describe an unusual presentation of clonorchis sinensis infection with obstructive jaundice due to duodenal papillitis which was relieved dramatically by endoscopic sphincterotomy. a 26-yr-old male presented with complaints of fatigue, weight loss and painless jaundice. the history was significant for frequent ingestion of raw freshwater fish. the patient underwent endoscopic retrograde cholangiopancreatography for evaluation of obstructive jaundice. the duodenal papilla was markedly edematou ... | 2010 | 21218042 |
| early detection and estimation of infection burden by real-time pcr in rats experimentally infected with clonorchis sinensis. | a real-time pcr assay targeting the cytochrome c oxidase gene of clonorchis sinensis was developed and its diagnostic value was investigated in experimentally infected rats. the pcr assay amplified as little as 0.1 pg of c. sinensis genomic dna and a single egg from experimentally spiked negative feces. the assay could detect c. sinensis dna in feces 2 weeks post-infection (pi), whereas the kato-katz method and formalin-ether concentration techniques could detect the eggs in the feces from 3 wee ... | 2011 | 21279385 |
| clonorchis sinensis infestation: a case report. | a young female refugee from indochina suffered recurring episodes of upper abdominal pain for several months before developing acute cholangitis and pancreatitis, associated with the presence of ova of clonorchis sinensis in her stool. microscopy of her stools was negative for this parasite on several previous occasions. the attention of all family physicians caring for 'boat people' is drawn to the possibility of disease developing after arrival in canada, despite screening on arrival. | 1981 | 21289739 |
| rare appearance of clonorchis sinensis in england. | | 1942 | 21313262 |