| purification and characterization of a novel anticoagulant and fibrinolytic enzyme produced by endophytic bacterium paenibacillus polymyxa ejs-3. | endophytes may become a new source of thrombolytic agents for thrombosis treatment. | 2010 | 20813399 |
| characterization of heterotrophic nitrifying bacteria with respiratory ammonification and denitrification activity--description of paenibacillus uliginis sp. nov., an inhabitant of fen peat soil and paenibacillus purispatii sp. nov., isolated from a spacecraft assembly clean room. | in the course of studying the influence of n-fertilization on n(2) and n(2)o flux rates in relation to soil bacterial community composition of a long-term fertilization experiment in fen peat grassland, a strain group was isolated that was related to a strain isolated from a spacecraft assembly clean room during diversity studies of microorganisms, which withstood cleaning and bioburden reduction strategies. both the fen soil isolates and the clean room strain revealed versatile physiological ca ... | 2010 | 20813476 |
| [screening and identification of plant growth-promoting rhizobacteria]. | the interaction between plant growth promoting rhizobacteria (pgpr) and plants can be unstable, pgpr with pgp activities may be well adapted to particular soil environment. based on this, we isolated and identified pgprs from different rhizosphere soils according to their multiple mechanisms. | 2010 | 20815230 |
| 1h,1'h-[3,3']biindolyl from the terrestrial fungus gliocladium catenulatum. | | 2010 | 20823893 |
| interactive optimization of biosurfactant production by paenibacillus alvei arn63 isolated from an iranian oil well. | the potential of an indigenous bacterial strain isolated from an iranian oil field for the production of biosurfactant was investigated in this study. after isolation, the bacterium was characterized to be paenibacillus alvei by biochemical tests and 16s ribotyping. the biosurfactant, which was produced by this bacterium, was able to lower the surface tension of media to 35 mn/m. accordingly, thin layer chromatography (tlc) and ft-ir has been carried out to determine compositional analysis of th ... | 2011 | 20846835 |
| genome sequence of the polymyxin-producing plant-probiotic rhizobacterium paenibacillus polymyxa e681. | paenibacillus polymyxa e681, a spore-forming, low-g+c, gram-positive bacterium isolated from the rhizosphere of winter barley grown in south korea, has great potential for agricultural applications due to its ability to promote plant growth and suppress plant diseases. here we present the complete genome sequence of p. polymyxa e681. its 5.4-mb genome encodes functions specialized to the plant-associated lifestyle and characteristics that are beneficial to plants, such as the production of a pla ... | 2010 | 20851896 |
| evidence of the involvement of asparagine deamidation in the formation of cyclodextrin glycosyltransferase isoforms in paenibacillus sp. rb01. | cyclodextrin glycosyltransferase (cgtase) from paenibacillus sp. rb01 and its recombinant enzyme exhibit three isoforms (i, ii, and iii) with the same apparent size but different charge. here, we demonstrate for the first time that the deamidation of labile asns causes the change in molecular forms of cgtase. the faster increase in number of isoforms was observed upon incubation in deamidation buffer at the more alkaline ph. the increase in levels of isoform ii and iii over time correlated with ... | 2011 | 20865347 |
| stimulation of defense reactions in medicago truncatula by antagonistic lipopeptides from paenibacillus sp. strain b2. | with the aim of obtaining new strategies to control plant diseases, we investigated the ability of antagonistic lipopolypeptides (paenimyxin) from paenibacillus sp. strain b2 to elicit hydrogen peroxide (h₂o₂) production and several defense-related genes in the model legume medicago truncatula. for this purpose, m. truncatula cell suspensions were used and a pathosystem between m. truncatula and fusarium acuminatum was established. in m. truncatula cell cultures, the induction of h₂o₂ reached a ... | 2010 | 20870792 |
| reclassification of paenibacillus ginsengisoli as a later heterotypic synonym of paenibacillus anaericanus. | the type strains of the species paenibacillus ginsengisoli (kctc 13931t) and paenibacillus anaericanus (dsm 15890t) were compared in order to clarify the taxonomic relationship of the two species. on the basis of 16s rrna, gyrb and rpob gene sequence comparisons, the two strains shared 99.9, 100 and 99.4 % similarity, respectively. the mean dna-dna relatedness value was 77 % and the genomic dna g+c contents were 43.2 and 42.2 mol%, respectively. phenotypic data, including fatty acid patterns and ... | 2010 | 20870883 |
| isolation of tn1546-like elements in vancomycin-resistant enterococcus faecium isolated from wood frogs: an emerging risk for zoonotic bacterial infections to humans. | isolation and characterization of vancomycin-resistant enterococci (vre), mainly enterococcus faecium, from the faecal pellet of wood frogs (rana sylvatica). | 2010 | 20880145 |
| bacterial community diversity in the brazilian atlantic forest soils. | the aim of this study was to characterize the bacterial community diversity of the brazilian atlantic forest soil by means of both cultivation and 16s rrna clone libraries. a collection of 86 representative isolates, obtained from six samples of atlantic forest soils from the national park of serra dos órgãos (parnaso), belonged to the genera arthrobacter, bacillus, burkholderia, leifsonia, paenibacillus, pseudomonas, ralstonia, serratia, and streptomyces according to the 16s rrna sequences. rep ... | 2010 | 20886336 |
| paenibacillus algorifonticola sp. nov., isolated from a cold spring in china. | a gram-positive, endospore-forming, rod-shaped bacterium, designated xj259t was isolated from a cold spring sample from xinjiang uyghur autonomous region, china. the isolate grew optimally at 20-30 °c and ph 7.3-7.8. comparative analysis of the 16s rrna gene sequence showed that isolate xj259t phylogenetically belonged to the genus paenibacillus, and most closely related to paenibacillus xinjiangensis b538t (with 96.6 % similarity), paenibacillus glycanilyticus ds-1t (96.3 %) and paenibacillus c ... | 2010 | 20889764 |
| complete genome sequence of paenibacillus polymyxa sc2, a strain of plant growth-promoting rhizobacterium with broad-spectrum antimicrobial activity. | paenibacillus polymyxa sc2 is an important plant growth-promoting rhizobacterium (pgpr). here, we report the complete genome sequence of p. polymyxa sc2. multiple sets of functional genes have been found in the genome. as far as we know, this is the first complete genome sequence of paenibacillus polymyxa. | 2010 | 21037012 |
| purification and characterization of chitinase from paenibacillus sp. d1. | a 56.56-kda extracellular chitinase from paenibacillus sp. d1 was purified to 52.3-fold by ion exchange chromatography using sp sepharose. maximum enzyme activity was recorded at ph 5.0 and 50 °c. maldi-lc-ms/ms analysis identified the purified enzyme as chitinase with 60% similarity to chitinase chi55 of paenibacillus ehimensis. the activation energy (e (a)) for chitin hydrolysis and temperature quotient (q (10)) at optimum temperature was found to be 19.14 kj/mol and 1.25, respectively. determ ... | 2010 | 21049291 |
| novel sigmab regulation modules of gram-positive bacteria involve the use of complex hybrid histidine kinases. | a common bacterial strategy to cope with stressful conditions is the activation of alternative sigma factors that control specific regulons enabling targeted responses. in the human pathogen bacillus cereus, activation of the major stress-responsive sigma factor σ(b) is controlled by a signalling route that involves the multi-sensor hybrid histidine kinase rsbk. rsbk-type kinases are not restricted to the b. cereus group, but occur in a wide variety of other bacterial species, including members ... | 2010 | 21051490 |
| characterization of paenibacillus curdlanolyticus intracellular xylanase xyn10b encoded by the xyn10b gene. | the paenibacillus curdlanolyticus xyn10b gene encoding a family-10 xylanase was cloned and expressed in escherichia coli, and the recombinant enzyme rxyn10b was characterized. immunological analysis suggested that xyn10b is an intracellular enzyme. rxyn10b hydrolyzed birch-wood xylan and xylooligosaccharides to produce mainly xylobiose, suggesting that it is an endoxylanase. its properties were significantly different from those of some homologous enzymes. | 2010 | 21071839 |
| identification of β-propeller phytase-encoding genes in culturable paenibacillus and bacillus spp. from the rhizosphere of pasture plants on volcanic soils. | phytate is one of the most abundant sources of organic phosphorus (p) in soils, but must be mineralized by phytase-producing bacteria to release p for plant uptake. microbial inoculants based on bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. here, we studied the diversity of aerobic endospore-forming bacteria (efb) with the ability to mineralize phytate in the rhizosphere o ... | 2010 | 21073489 |
| phytoremediation of metals from fly ash through bacterial augmentation. | different combinations of four bacterial strains isolated from fly ash were used by us to study their impact on phytoextraction of metals from fly ash by brassica juncea grown in fly ash amended with farm yard manure (50:50 w/w). out of 11 bacterial consortia, a combination of two strains i.e. paenibacillus macerans nbrft5 + bacillus pumilus nbrft9 (c7) inoculated in the rhizosphere was found to enhance pb accumulation maximally by 278%, mn by 75%, zn by 163%, cr by 226% and ni by 414% compared ... | 2010 | 21080221 |
| [fusion expression of fibrinolytic enzyme gene ppfe-i from endophytic paenibacillus polymyxa in escherichia coli and activity analysis]. | with the genomic dna of strain ejs-3 as the template, we amplified the gene of fibrinolytic enzyme from paenibacillus polymyxa (ppfe-i) by pcr. we purified the pcr product and ligated it into pmd19-t. after dna sequencing, we cloned the ppfe-i gene into expression vector pet-dsba and transformed it into escherichia coli bl21(de3). upon induction of iptg, we found that the activity of recombinant fibrinolytic enzyme fused with dsba expressed in escherichia coli was 228 iu/ml. sds-page analysis sh ... | 2010 | 21090119 |
| harvest of scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. | the optimal flocculating conditions for harvesting high-density cultures of scenedesmus sp. were investigated using inorganic coagulants and the bioflocculant produced by paenibacillus polymyxa am49. the flocculated medium as nutrients for subsequent algal cultivation was also tested. consecutive treatment with 8.5 mm cacl(2) and 0.2 mm fecl(3) as coagulants and 1% bioflocculant from the culture broth of p. polymyxa am49 showed the highest flocculating activity of up to 95% for high density alga ... | 2010 | 21094603 |
| study of a novel glycoconjugate, thiopeptidoglycan, and a novel polysaccharide lyase, thiopeptidoglycan lyase. | a typical filamentous bacterium, sphaerotilus natans, secretes a thiolic glycoconjugate which is assembled into a microtube, so called sheath. the glycoconjugate is known to consist of a pentasaccharide-dipeptide repeating unit, but its chemical structure has not been completely elucidated. in order to determine its chemical structure, the sheath was broken down by performic acid oxidation. the released sulfonated derivative was water soluble which was suitable for detailed nmr analysis. the dat ... | 2010 | 21095202 |
| paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications. | the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ... | 2010 | 21132276 |
| [genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove]. | we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability. | 2010 | 21141471 |
| effects of plant growth promoting rhizobacteria (pgpr) on rooting and root growth of kiwifruit (actinidia deliciosa) stem cuttings. | the effects of plant growth promoting rhizobacteria (pgpr) on the rooting and root growth of semi-hardwood and hardwood kiwifruit stem cuttings were investigated. the pgpr used were bacillus rc23, paenibacillus polymyxa rc05, bacillus subtilis osu142, bacillus rc03, comamonas acidovorans rc41, bacillus megaterium rc01 and bacillus simplex rc19. all the bacteria showed indole-3-acetic acid (iaa) producing capacity. among the pgpr used, the highest rooting ratios were obtained at 47.50% for semi-h ... | 2010 | 21157636 |
| cell surface display of cellulase activity-free xylanase enzyme on saccharomyces cerevisiae eby100. | cellulase-free xylanase has potential for its application in the selective removal of hemicellulose from kraft pulp to give good strength to paper. in this study, a gene (xyn) encoding cellulase activity-free xylanase enzyme (xyn) was isolated from paenibacillus polymyxa ppl-3. the xyn gene encoded a protein of 221 amino acids, and the purified xyn was about 22.5 kda measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. moreover, the cellulase activity-free xylanase enzyme (xyn) ... | 2010 | 21161608 |
| genome sequence of the pattern forming paenibacillus vortex bacterium reveals potential for thriving in complex environments. | the pattern-forming bacterium paenibacillus vortex is notable for its advanced social behavior, which is reflected in development of colonies with highly intricate architectures. prior to this study, only two other paenibacillus species (paenibacillus sp. jdr-2 and paenibacillus larvae) have been sequenced. however, no genomic data is available on the paenibacillus species with pattern-forming and complex social motility. here we report the de novo genome sequence of this gram-positive, soil-dwe ... | 2010 | 21167037 |
| production of a newly isolated paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater. | a phyllosphere bacterial strain ebl-06 was isolated from wheat leaves. the morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. a phylogenetic tree was constructed by comparing with the published 16s rdna sequences of the relevant bacteria. the results showed that the isolate ebl-06 was a strain of paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad ... | 2010 | 21174972 |
| assessment of root-associated paenibacillus polymyxa groups on growth promotion and induced systemic resistance in pepper. | twenty-nine p. polymyxa strains isolated from rhizospheres of various crops were clustered into five genotypic groups on the basis of box-pcr analysis. the characteristics of several plant growth-promoting factors among the isolates revealed the distinct attributes in each allocated group. under gnotobiotic conditions, inoculation of pepper roots with p. polymyxa isolates significantly increased the biomass in 17 of total 29 treated plants with untreated plants. experiments on induced systemic r ... | 2010 | 21193814 |
| characterization of a paenibacillus woosongensis beta-xylosidase/alpha-arabinofuranosidase produced by recombinant escherichia coli. | a gene encoding the beta-xylosidase/alpha-arabinofuranosidase (xylc) of paenibacillus woosongensis was cloned into escherichia coli. this xylc gene consisted of 1,425 nucleotides, encoding a polypeptide of 474 amino acid residues. the deduced amino acid sequence exhibited an 80% similarity with those of both clostridium stercorarium beta-xylosidase/alpha-n-arabinosidase and bacillus cellulosilyticus alpha-arabinofuranosidase, belonging to the glycosyl hydrolase family 43. the structural gene was ... | 2010 | 21193828 |
| paenibacillus wooponensis sp. nov., isolated from wetland freshwater. | a rod-shaped enodospore-forming gram-positive bacterium, strain wpcb018t, was isolated from a fresh water sample collected from woopo wetland, korea. the isolate was identified as a member of the genus paenibacillus on the basis of phenotypic characteristics and phylogenetic inference based on 16s rrna gene sequence. the major polar lipids are diphosphatidylglycerol, phosphatidylethanolamine and unknown aminophospholipids. the diamino acid found in the cell wall peptidoglycan is meso-diaminopime ... | 2011 | 21216920 |
| paenibacillus sacheonensis sp. nov., a xylanolytic and cellulolytic bacterium isolated from tidal flat sediment in sacheon bay, korea. | a gram-negative, rod-shaped bacterium, designated strain sy01(t) was isolated from tidal flat sediment of the sacheon bay in south korea. strain sy01(t) was characterized with respect to their phenotypic and phylogenetic characteristics. this strain was spore-forming, motile, catalase-negative and oxidase-positive. optimal growth of the strain occurred at 30°c and ph 7.0. the dna g+c content was 56.1 mol%. the predominant menaquinone was mk-7. diphosphatidylglycerol, phosphatidylglycerol, phosph ... | 2011 | 21216922 |
| [binding of tylosin, tilmicosin and oxytetracycline to proteins from honeybees, larvae and beehive products]. | american foulbrood (afb) caused by the spore-forming bacterium paenibacillus larvae is the most serious disease of bacterial origin affecting larvae and pupae of honeybees. antibiotics are used in many countries for the control of afb in high incidence areas, but their misuse may lead to antibiotic resistance of bacterial strains and honey contamination. the objective of the present work was to determine, through a biological method, the protein binding of tylosin, tilmicosin and oxytetracycline ... | 2010 | 21229198 |
| [optimization immobilizing activated carbon and bacteria by sodium alginate and its character of adsorption of pb2+]. | through the immobilized biosorption of activated carbon and paenibacillus polymyxa ga1 by sodium alginate, this paper take advantage of the orthogonal experiment to investigate the adsorption characteristics of sodium alginate. according to the research, the optimal preparation conditions were as following: the mass fraction of sodium alginate was 2.5%; the amount of carbon and bacteria was 1:20 and 1:2, respectively; under the preparation condition, the adsorption rate could reach to 93.74%. th ... | 2010 | 21250452 |
| molecular cloning and catalytic mechanism of a novel glycosphingolipid-degrading beta-n-acetylgalactosaminidase from paenibacillus sp. ts12. | we report here the molecular cloning, characterization, and catalytic mechanism of a novel glycosphingolipid-degrading ß-n-acetylgalactosaminidase (ß-nga) from paenibacillus sp. ts12 (ngap). consisting of 1034 putative amino acid residues, ngap shares no sequence similarity with known proteins. recombinant ngap, expressed in escherichia coli, cleaved the nonreducing terminal ß-galnac residues of gangliotriaosylceramide and globotetraosylceramide. the enzyme hydrolyzed para-nitrophenyl-ß-n-acetyl ... | 2011 | 21297160 |
| diversity of endophytic bacteria within nodules of the sphaerophysa salsula in different regions of loess plateau in china. | a total of 115 endophytic bacteria were isolated from root nodules of the wild legume sphaerophysa salsula grown in two ecological regions of loess plateau in china. the genetic diversity and phylogeny of the strains were revealed by restriction fragment length polymorphism and sequencing of 16s rrna gene and enterobacterial repetitive intergenic consensus-pcr. their symbiotic capacity was checked by nodulation tests and analysis of nifh gene sequence. this is the first systematic study on endop ... | 2011 | 21303396 |
| extremely variable conservation of ?-type small, acid-soluble proteins from spores of some species in the bacterial order bacillales. | ?-type small, acid-soluble spore proteins (sasp) are the most abundant proteins in spores of at least some members of the bacterial order bacillales, yet they remain an enigma from both functional and phylogenetic perspectives. current work has shown that the ?-type sasp or their coding genes (sspe genes) are present in most spore-forming members of bacillales, including at least some members of the paenibacillus genus, although they are apparently absent from clostridiales species. we have appl ... | 2011 | 21317325 |
| [screening, identification, and biocontrol effect of antagonistic bacteria against phytophthora capsici]. | a total of 98 isolates with antagonistic activity against phytophthora capsici were isolated from the rhizosphere soil of healthy pepper plants in the fields seriously infected by pepper phytophthora capsicit, and two strains named as hl-3 and lz-8 were screened, which had the characteristics of wide-spectrum antagonism and good growth under poor soil condition. the hl-3 and lz-8 were identified as paenibacillus polymyxa and bacillus pumilus, respectively, based on their morphological and bioche ... | 2010 | 21328956 |
| metalloprotease production by paenibacillus larvae during the infection of honeybee larvae. | american foulbrood is a bacterial disease of worldwide distribution that affects larvae of the honeybee apis mellifera. the causative agent is the gram-positive, spore-forming bacterium paenibacillus larvae. several authors have proposed that p. larvae secretes metalloproteases that are involved in the larval degradation that occurs after infection. the aim of the present work was to evaluate the production of a metalloprotease by p. larvae during larval infection. first, the complete gene encod ... | 2011 | 21330433 |
| biofilm formation by paenibacillus polymyxa strains differing in the production and rheological properties of their exopolysaccharides. | we evaluated the ability of several strains of the rhizobacterium paenibacillus polymyxa, differing in the yield and rheological properties of their exopolysaccharides, to form biofilms on abiotic surfaces. of these strains, p. polymyxa 1465, giving the highest yield of extracellular polysaccharides and the highest kinematic viscosity of the culture liquid and of aqueous polysaccharide solutions, proved to be the most active in forming biofilms on hydrophobic and hydrophilic surfaces. enzyme-lin ... | 2011 | 21331554 |
| characterization of root-nodulating bacteria associated to prosopis farcta growing in the arid regions of tunisia. | diversity of 50 bacterial isolates recovered from root nodules of prosopis farcta grown in different arid soils in tunisia, was investigated. characterization of isolates was assessed using a polyphasic approach including phenotypic characteristics, 16s rrna gene pcr--rflp and sequencing, noda gene sequencing and mlsa. it was found that most of isolates are tolerant to high temperature (40°c) and salinity (3%). genetic characterization emphasizes that isolates were assigned to the genus ensifer ... | 2011 | 21359955 |
| isolation and characterization of beneficial bacteria associated with citrus roots in florida. | cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. citrus roots from huanglongbing (hlb) diseased symptomatic and asymptomatic citrus were used in this study. a total of 227 and 125 morphologically distinct colonies were isolated and characterized from hlb asymptomatic and symptomatic trees, respectively. we obs ... | 2011 | 21360139 |
| screening of endophytic bacteria isolated from leaves of sambung nyawa [gynura procumbens (lour.) merr.] for cytokinin-like compounds. | endophytic bacteria are harmless in most plant species; and known to boost the growth and development of the host plants probably by secreting growth hormones. the isolation, identification and screening of endophytic bacteria for the plant growth regulators like cytokinin are needed to get the leads for their applications in agriculture sector. we describe the isolation and identification of the bacterial endophytes from the leaves of sambung nyawa [gynura procumbens (lour.) merr.] and their sc ... | 2010 | 21364796 |
| paenimacrolidin, a novel macrolide antibiotic from paenibacillus sp. f6-b70 active against methicillin-resistant staphylococcus aureus. | paenibacillus sp. f6-b70 was selected from several dozens of isolates with activity against methicillin-resistant staphylococcus aureus using a 16s rdna-based screening method. f6-b70 contained polyketide synthase (pks) and non-ribosomal peptide synthetase (nrps) clusters in its genome revealed by pcr amplification of conserved adenylation and ketosynthase (ks) domains. phylogenetic data suggested that the strain hosts trans-at pkss and their product may be a branched molecule. an antibiotic was ... | 2010 | 21375709 |
| identification of li-f type antibiotics and di-n-butyl phthalate produced by paenibacillus polymyxa. | paenibacillus polymyxa strain jsa-9, a soil isolate that displayed antibacterial and antifungal activities in vitro, had been found to produce two types of antimicrobial substances. the two compounds were extracted from the fermentation broth of jsa-9 using ethyl acetate and subsequently purified by high performance liquid chromatography. by means of liquid chromatography-mass spectrometry and tandem mass spectrometry analysis, one of two antagonistic compounds was determined as di-n-butyl phtha ... | 2011 | 21376758 |
| optimization, purification, characterization and antioxidant activity of an extracellular polysaccharide produced by paenibacillus polymyxa sqr-21. | the optimization, purification and characterization of an extracellular polysaccharide (eps) from a bacterium paenibacillus polymyxa sqr-21 (sqr-21) were investigated. the results showed that sqr-21 produced one kind of eps having molecular weight of 8.96 +ù 10(5)da. the eps was comprised of mannose, galactose and glucose in a ratio of 1.23:1.14:1. the ratio of monosaccharides and glucuronic acid was 7.5:1. the preferable culture conditions for eps production were ph 6.5, temperature 30-¦c for 9 ... | 2011 | 21392978 |
| production and characterization of exopolysaccharides and antioxidant from paenibacillus sp. tku023. | using squid pen powder (spp) as the sole c/n source, paenibacillus sp. tku023 produced exopolysaccharides (eps) and antioxidant. with medium containing 1.5% spp, 0.1% k(2)hpo(4), and 0.05% mgso(4)·7h(2)o, ph 7.23, the culture was incubated at 37°c in liquid (50ml/250ml) for five days. the resultant culture supernatant had higher eps productivity (4.55g/l). the crude eps were isolated by centrifugation, methanol precipitation and deproteinization. the characterization of the eps demonstrated that ... | 2011 | 21402186 |
| high-level extracellular production of α-cyclodextrin glycosyltransferase with recombinant escherichia coli bl21 (de3). | high-level production of α-cyclodextrin glycosyltransferase (cgtase) is one of the key factors in α-cyclodextrin (cd) preparation. in the present study, a fed-batch fermentation strategy for high-cell-density cultivation of escherichia coli and the extracellular production of recombinant α-cgtase from paenibacillus macerans jfb05-01 was established. a combined feeding strategy based on both specific growth rate before induction and the amount of glycerol residues after induction was used to cont ... | 2011 | 21417392 |
| effect of enzyme producing microorganisms on the biomass of epigeic earthworms (eisenia fetida) in vermicompost. | we analyzed the bacterial community structure of the intestines of earthworms and determined the effect of enzyme producing microorganisms on the biomass of earthworms in vermicompost. fifty-seven bacterial 16s rdna clones were identified in the intestines of earthworms by using polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) analysis. entomoplasma somnilux and bacillus licheniformis were the dominant microorganisms; other strains included aeromonas, bacillus, clostr ... | 2011 | 21421302 |
| impact of chlorophenols on microbiota of an unpolluted acidic soil: microbial resistance and biodegradation. | the impact of 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp) on the microbial community of an acidic forest soil was studied under controlled laboratory conditions by spiking microcosms with the pollutants at concentrations ranging from 0.1 to 5000 mg kg(-1) . a decrease in the cumulative respirometric values and changes in the bacterial and fungal community composition were detected at 1000 mg mcp kg(-1) , 100 mg tcp kg(-1) and 100 and 1000 mg pcp kg(-1) . how ... | 2011 | 21426365 |
| structural basis for the inhibition of 1,3-1,4-ß-d-glucanase by noncompetitive calcium ion and competitive tris inhibitors. | in this paper, we determine the mutant w203f structure of tfsß-glucanase, which contains aromatic residue trp203 at the active site of the enzyme. residue trp203 is stacked with the glucose product of cellotriose. further analysis reveals that two extra calcium ions and a tris molecule bind to the mutant structure. a tris molecule, bound to the catalytic residues of glu56 and glu60, was found at the position normally taken by substrate binding at the -1 subsite. in addition, a second ca(2+) ion ... | 2011 | 21426903 |
| antibacterial activity of metabolite produced by paenibacillus polymyxa strain hka-15 against xanthomonas campestris pv. phaseoli. | an antibacterial metabolite extracted from paenibacillus polymyxa hka-15 showed strong inhibition against xanthomonas campestris pv. phaseoli strains cp-1-1 and m-5. minimum inhibitory concentration (mic) of crude extract against strains cp-1-1 and m-5 was found to be 1.7 mg/ml and 1.52 mg/ml, respectively. in uv-vis range, the absorption peak of crude extract was maximum at 240 nm. the compound is resilience to wide range of temperature, ph, surfactants and organic solvents. the complete loss o ... | 2011 | 21452603 |
| diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan. | a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ... | 2011 | 21487197 |
| genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden. | very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ... | 2011 | 21497473 |
| determination of tylosins a, b, c and d in bee larvae by liquid chromatography coupled to ion trap-tandem mass spectrometry. | a lc-ms/ms method has been developed to simultaneously quantify tylosins a, b, c and d in bee larvae, compounds currently used to treat one of the most lethal diseases affecting honey bees around the world, american foulbrood (afb). the influence of different aqueous media, temperature and light exposure on the stability of these four compounds was studied. the analytes were extracted from bee larvae with methanol and chromatographic separation was achieved on a luna c(18) (150×4.6mm i.d.) using ... | 2011 | 21498134 |
| novel biphenyl-oxidizing bacteria and dioxygenase genes from a korean tidal mudflat. | gene-targeted flx titanium pyrosequencing integrated with stable isotope probing (sip) using [(13)c]biphenyl substrate revealed that tidal mudflat sediments harbor novel aromatic ring hydroxylating dioxygenases (arhd). more than 80% of the detected arhd genes comprise four clades (0.5 distance) with 49 to 70% amino acid identity to sequences in public databases. the 16s rrna sequences enriched in the (13)c fraction were from the betaproteobacteria, bacilli (primarily paenibacillus-like), and unc ... | 2011 | 21498748 |
| characterization of a paenibacillus curdlanolyticus b-6 xyn10d xylanase that contains a family 3 carbohydrate-binding module. | paenibacillus curdlanolyticus b-6 xyn10d is a xylanase containing a family 3 carbohydrate-binding module (cbm3). biochemical analyses using recombinant proteins derived from xyn10d suggested that the cbm3 polypeptide has an affinity for cellulose and xylan and that cbm3 in xyn10d is important for hydrolysis of insoluble arabinoxylan and natural biomass. | 2011 | 21498754 |
| seasonal change of bacterial community structure in a biofilm formed on the surface of the aquatic macrophyte phragmites australis. | the seasonal change of bacterial community structure in biofilms on the surface of reed (phragmites australis) was investigated for about three years (from 2005 june to 2008 march) in lake biwa by comparing it with that in surrounding lake water. the community structure in biofilms was different from that in the lake water throughout the seasons and years. the community structure in lake water was similar in the same seasons of different years, corresponding to similar environmental factors (i.e ... | 2011 | 21502742 |
| potential of ozone as a fumigant to control pests in honey bee (hymenoptera: apidae) hives. | ozone is a powerful oxidant capable of killing insects and microorganisms, and eliminating odors, taste, and color. thus, it could be useful as a fumigant to decontaminate honey comb between uses. the experiments here are intended to determine the exposure levels required to kill an insect pest and spore forming bee pathogens. ozone was effective against greater wax moth, galleria mellonella (l.) (lepidoptera: pyralidae), even on naturally infested comb. neonates and adults were the easiest life ... | 2011 | 21510179 |
| identification of nitrogen-fixing paenibacillus from different plant rhizospheres and a novel nifh gene detected in the p. stellifer. | a total of 534 isolates were selectively obtained from different plant rhizospheres based on their growth on nitrogen-free medium and their resistance to 80 degrees c for 15 min. of the 534 isolates, 23 isolates had nifh gene and exhibited nitrogenase activities. based on 16s rdna sequence, g + c content assay and dna-dna hybridization, by the 23 isolates, which were divided into four monophyletic clusters, all belonged to the paenibacillus genus. nifh gene deduced amino acid alignment analysis ... | 2011 | 21513217 |
| a novel (2r,3r)-2,3-butanediol dehydrogenase from an industrially potential strain paenibacillus polymyxa atcc12321. | a (2r,3r)-2,3-butanediol dehydrogenase (bdh99::67) from paenibacillus polymyxa atcc12321 was functionally characterized. the genetic characteristic of bdh99::67 is completely different from those of meso- and (2s,3s)-2,3-butanediol dehydrogenases. the results showed bdh99::67 belongs to the medium-chain dehydrogenase/reductase superfamily but not to the short-chain dehydrogenase/reductase superfamily, which meso- and (2s,3s)-2,3-butanediol dehydrogenases belong to. | 2011 | 21531839 |
| a novel low-temperature-active β-glucosidase from symbiotic serratia sp. tn49 reveals four essential positions for substrate accommodation. | a 2,373-bp full-length gene (bgla49) encoding a 790-residue polypeptide (bgla49) with a calculated mass of 87.8 kda was cloned from serratia sp. tn49, a symbiotic bacterium isolated from the gut of longhorned beetle (batocera horsfieldi) larvae. the deduced amino acid sequence of bgla49 showed the highest identities of 80.1% with a conceptually translated protein from pantoea sp. at-9b (eew02556), 38.3% with the identified glycoside hydrolase (gh) family 3 β-glucosidase from clostridium stercora ... | 2011 | 21559826 |
| population dynamics of bacteria associated with different strains of the pine wood nematode bursaphelenchus xylophilus after inoculation in maritime pine (pinus pinaster). | for a long time it was thought that bursaphelenchus xylophilus was the only agent of the pine wilt disease. recently, it was discovered that there are bacteria associated with the nematodes that contribute to the pathogenesis of this disease, mainly through the release of toxins that promote the death of the pines. among the species most commonly found, are bacteria belonging to the bacillus, pantoea, pseudomonas and xanthomonas genera. the main objective of this work was to study the effect of ... | 2011 | 21570967 |
| maturation of bone marrow-derived dendritic cells by a novel β-glucan purified from paenibacillus polymyxa jb115. | we investigated the immunostimulatory effects of a novel β-glucan purified from paenibacillus (p.) polymyxa jb115 on bone marrow-derived dendritic cells (dcs), a type of potent antigen-presenting cells. β-glucan isolated from p. polymyxa jb115 enhanced the viability and induced the maturation of dcs. β-glucan markedly increased the cytokine production of dcs and surface expression of dc markers. in addition, dcs treated with β-glucan showed a higher capacity to stimulate allogeneic spleen cell p ... | 2011 | 21586879 |
| the smartest bacteria on earth. | | 2011 | 21608406 |
| redox properties of 8-quinolinol and implications for its mode of action. | 8-quinolinol (oxine, 8-hydroxyquinoline) is a simple aromatic alkaloid with allelopathic, antibacterial, antifungal, and cytotoxic activities. generally, it is assumed that 8-quinolinol toxicity depends on transition metal chelation that negatively affects their availability for metalloenzymes in the cell or reactive oxygen species generation (ros), which are formed following reduction of molecular oxygen by autoxidation of the redox active metal central atom of the 8-quinolinol complex. on the ... | 2011 | 21615015 |
| building custom polysaccharides in vitro with an efficient, broad-specificity xyloglucan glycosynthase and a fucosyltransferase. | the current drive for applications of biomass-derived compounds, for energy and advanced materials, has led to a resurgence of interest in the manipulation of plant polymers. the xyloglucans, a family of structurally complex plant polysaccharides, have attracted significant interest due to their intrinsic high affinity for cellulose, both in muro and in technical applications. moreover, current cell wall models are limited by the lack of detailed structure-property relationships of xyloglucans, ... | 2011 | 21618981 |
| indole and 3-indolylacetonitrile inhibit spore maturation in paenibacillus alvei. | abstract: background: bacteria use diverse signaling molecules to ensure the survival of the species in environmental niches. a variety of both gram-positive and gram-negative bacteria produce large quantities of indole that functions as an intercellular signal controlling diverse aspects of bacterial physiology. results: in this study, we sought a novel role of indole in a gram-positive bacteria paenibacillus alvei that can produce extracellular indole at a concentration of up to 300 microm in ... | 2011 | 21619597 |
| thermal denaturation of β-glucosidase b from paenibacillus polymyxa proceeds through a lumry-eyring mechanism. | β-glucosidase b (bglb), 1,4-β-d: -glucanohydrolase, is an enzyme with various technological applications for which some thermostable mutants have been obtained. because bglb denatures irreversibly with heating, the stabilities of these mutants are assessed kinetically. it, therefore, becomes relevant to determine whether the measured rate constants reflect one or several elementary kinetic steps. we have analyzed the kinetics of heat denaturation of bglb from paenibacillus polymyxa under various ... | 2011 | 21626159 |
| surviving bacterial sibling rivalry: inducible and reversible phenotypic switching in paenibacillus dendritiformis. | abstract natural habitats vary in available nutrients and room for bacteria to grow, but successful colonization can lead to overcrowding and stress. here we show that competing sibling colonies of paenibacillus dendritiformis bacteria survive overcrowding by switching between two distinct vegetative phenotypes, motile rods and immotile cocci. growing colonies of the rod-shaped bacteria produce a toxic protein, slf, which kills cells of encroaching sibling colonies. however, sublethal concentrat ... | 2011 | 21628502 |
| synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect-gut bacterium. | insects living on wood and plants harbor a large variety of bacterial flora in their guts for degrading biomass. we isolated a paenibacillus strain, designated as icgeb2008, from the gut of a cotton bollworm, on the basis of its ability to secrete a variety of plant hydrolyzing enzymes. in this study, we cloned, expressed and characterized two enzymes, β-1,4-endoglucanase (endo5a) and β-1,4-endoxylanase (xyl11d), from the icgeb2008 strain and synthesized recombinant bifunctional enzymes based on ... | 2011 | 21642416 |
| [enhanced storage stability of recombinant enzyme preparation of alpha-cgtase from paenibacillus macerans by chemical additives]. | to enhance the thermostability and storage stability of alpha-cyclodextrin glycosyltransferase (a-cgtase), we added specific chemical additives into the preparation of alpha-cgtase, and studied the effect of additives on the storage stability of alpha-cgtase at different temperatures. then we measured the protein structure of cgtase in the far uv (200-250 nm) and near uv (250-320 nm) ranges respectively by circular dichroism (cd) spectra under high temperature and analyzed the relationship betwe ... | 2011 | 21650042 |
| biodegradation of enteromorpha prolifera by mangrove degrading micro-community with physical-chemical pretreatment. | the bacteria involved in the biodegradation of enteromorpha prolifera (ep) are largely unknown, especially in offshore mangrove environments. in order to obtain the bacterial ep-degrading communities, sediments from a typical mangrove forest were sampled on the roots of mangrove in dongzhai port (haikou, china). the sediments were enriched with crude ep powders as the sole carbon source. the bacterial composition of the resulting mangrove-degrading micro-community (mdmc), named d2-1, was analyse ... | 2011 | 21660543 |
| conservation and diversity of seed associated endophytes in zea across boundaries of evolution, ethnography and ecology. | endophytes are non-pathogenic microbes living inside plants. we asked whether endophytic species were conserved in the agriculturally important plant genus zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from mexico to canada. kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and dna fingerprinting using terminal restriction fragment length polymorphis ... | 2011 | 21673982 |
| structural and functional analysis of phytotoxin toxoflavin-degrading enzyme. | pathogenic bacteria synthesize and secrete toxic low molecular weight compounds as virulence factors. these microbial toxins play essential roles in the pathogenicity of bacteria in various hosts, and are emerging as targets for antivirulence strategies. toxoflavin, a phytotoxin produced by burkholderia glumae bgr1, has been known to be the key factor in rice grain rot and wilt in many field crops. recently, toxoflavin-degrading enzyme (txde) was identified from paenibacillus polymyxa jh2, there ... | 2011 | 21799856 |
| structure and activity of a paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44. | the enzymatic degradation of plant polysaccharides is emerging as one of the key environmental goals of the early twenty-first century; impacting on many processes in the textile and detergent industries as well as biomass conversion to biofuels. one of the well-known problems with the use of non-starch (non-food) based substrates such as the plant cell wall is that the cellulose fibres are embedded in a network of diverse polysaccharides including xyloglucan that render access difficult. there ... | 2011 | 21795708 |
| paenibacillus sediminis sp. nov., a xylanolytic bacterium isolated from tidal flat adjacent to ganghwa island, korea. | a gram-positive, rod-shaped, xylanolytic, spore-forming bacterium, gth-3(t), was isolated from tidal flat in ganghwa island (n37° 42' 02", e126° 22' 38"), republic of korea and characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity, strain gth-3(t) was shown to belong to the family paenibacillaceae, being related to paenibacillus ginsengisoli (94.9%), paenibacillus anaericanus (94.8%), paenibacillus urinalis (94.4%), paenibacillus cookii (94 ... | 2011 | 21784963 |
| a novel gfp-based approach for screening biocontrol microorganisms in vitro against dothistroma septosporum. | dothistroma septosporum is the causal agent of dothistroma needle blight of pine trees. a novel green fluorescent protein (gfp)-based screening method was developed to assess the potential of microorganisms for biocontrol of dothistroma. the screen utilizes gfp expression as an indicator of metabolic activity in the pathogen and hygromycin resistance selection to determine if the interaction is fungistatic or fungicidal. results suggested that six of eight trichoderma isolates tested have the po ... | 2011 | 21777628 |
| [controlling effect of antagonist bioorganic fertilizer on tomato root-knot nematode]. | indoor in vitro culture experiment and greenhouse pot experiment were conducted to evaluate the capabilities of three bacterial strains xz-173 (bacillus amyloliquefaciens), sl-25 (b. gibsonii), and ks-62 (paenibacillus polymyxa) that can hydrolyze collagen protein in controlling tomato root-knot nematode. in the in vitro culture experiment, suspensions of xz-173, sl-25, and ks-62 induced a mortality rate of 75.9%, 66.7%, and 50.0% to the second-stage junior nematode within 24 h, and decreased th ... | 2011 | 21774329 |
| diversity analysis of diazotrophic bacteria associated with the roots of tea (camellia sinensis (l.) o. kuntze). | the diversity elucidation by amplified ribosomal dna restriction analysis and 16s rdna sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of gram-positive over gram-negative bacteria within the kangra valley in himachal pradesh, india. the gram-positive bacteria observed belong to two taxonomic groupings; firmicutes, including the genera bacillus and paenibacillus; and actinobacteria, represented by ... | 2011 | 21715960 |
| entomopathogenic nematodes, phoretic paenibacillus spp., and the use of real time quantitative pcr to explore soil food webs in florida citrus groves. | quantitative real-time pcr (qpcr) is a powerful tool to detect and quantify species of cryptic organisms such as bacteria, fungi and nematodes from soil samples. as such, qpcr offers new opportunities to study the ecology of soil habitats by providing a single method to characterize communities of diverse organisms from a sample of dna. here we describe molecular tools to detect and quantify two bacteria (paenibacillus nematophilus and paenibacillus sp.) phoretically associated with entomopathog ... | 2011 | 21723288 |
| characterization of a microbial consortium capable of degrading lignocellulose. | a microbial consortium, designated wcs-6, was established by successive subcultivation in the presence of rice straw under static conditions. the degradation efficiencies of wsc-6 for 0.5g filter paper, cotton and rice straw after 3days of cultivation were 99.0-¦0.7%, 76.9-¦1.5% and 81.3-¦0.8%, respectively as determined by gravimetrical methods. nine bacterial isolates were obtained from wcs-6 plated under aerobic conditions, and sequencing of their 16s rdna indicated that these bacteria were r ... | 2011 | 21831630 |
| microbial oxidases catalyzing conversion of glycolaldehyde into glyoxal. | the present paper reviews oxidases catalyzing conversion of glycolaldehyde into glyoxal. the enzymatic oxidation of glycolaldehyde into glyoxal was first reported in alcohol oxidases (aods) from methylotrophic yeasts such as candida and pichia, and glycerol oxidase (glod) from aspergillus japonicus, although it had been reported that these enzymes are specific to short-chain linear aliphatic alcohols and glycerol, respectively. these enzymes continuously oxidized ethylene glycol into glyoxal via ... | 2011 | 21820089 |
| an outbreak of cutaneous infection due to mycobacterium abscessus associated to mesotherapy. | in february 2009 an outbreak of subcutaneous abscesses due to mycobacterium abscessus was detected in spain which affected healthy women who had undergone mesotherapy procedures in an aesthetic clinic. | 2011 | 21684045 |
| draft genome sequence of paenibacillus elgiib69, a strain with broad antimicrobial activity. | here, we report the draft genome sequence of paenibacillus elgiib69, which was isolated from soil and has broad-spectrum antimicrobial activity. as far as we know, the p. elgiigenome is the largest of the paenibacillusgenus for which genome sequences are available. multiple sets of genes related to antibiotic biosynthetic pathways have been found in the genome. | 2011 | 21705583 |
| radiation inactivation of paenibacillus larvae and sterilization of american foul brood (afb) infected hives using co-60 gamma rays. | the effectiveness of gamma radiation in inactivating the philippine isolate of paenibacillus larvae was investigated. spores of p. larvae were irradiated at incremental doses (0.1, 0.2, 0.4, 0.8 and 1.6kgy) of gamma radiation emitted by a (60)co source. surviving spores were counted and used to estimate the decimal reduction (d(10)) value. a dose of 0.2kgy was sufficient to inactivate 90% of the total recoverable spores from an initial count of 10(5)-9+ù10(3) spores per glass plate. the steriliz ... | 2011 | 21683605 |
| efficient expression of a paenibacillus barcinonensis endoglucanase in saccharomyces cerevisiae. | the endoglucanase coded by cela (genbank access no. y12512) from paenibacillus barcinonensis, an enzyme with good characteristics for application on paper manufacture from agricultural fibers, was expressed in saccharomyces cerevisiae by using different domains of the cell wall protein pir4 as translational fusion partners, to achieve either secretion or cell wall retention of the recombinant enzyme. given the presence of five potential n-glycosylation sites in the amino acid sequence coded by c ... | 2011 | 21701899 |
| draft genome sequence of the paenibacillus polymyxa type strain (atcc 842t), a plant growth-promoting bacterium. | paenibacillus polymyxa is an endospore-forming gram-positive soil bacterium that is well-known for its ability to promote plant growth. here we report the draft genome sequence of p. polymyxa atcc 842(t), the type strain of the species p. polymyxa, and the family paenibacillaceae. the p. polymyxa genome contains a repertoire of biosynthetic genes for antibiotics and hydrolytic enzymes that account for its beneficial effects in the rhizosphere to the host plants it associates with. | 2011 | 21742878 |
| paenibacillus chartarius sp. nov. isolated from a papermill. | a gram-positive, aerobic, endosporeforming organism, isolated from a papermill was studied for its taxonomic position. based on 16s rrna gene sequence similarity comparisons, strain ccug 55240t was grouped into the genus paenibacillus, most closely related to paenibacillus chinjuensis (93.7 %), paenibacillus elgii (93.7%), and paenibacillus chitinolyticus (93.6 %). the 16s rrna gene sequence similarity to other paenibacillus species was < 93.5 %, including the type species of this genus, paeniba ... | 2011 | 21828020 |
| genetic diversity of cultivable plant growth-promoting rhizobacteria in korea. | to elucidate the biodiversity of plant growth-promoting rhizobacteria (pgpr) in korea, 7,638 bacteria isolated from the rhizosphere of plant species growing in many different regions were screened. a large number of pgpr were identified by testing the ability of each isolate to promote the growth of cucumber seedlings. after redundant rhizobacteria were removed via amplified rdna restriction analysis, 90 strains were finally selected as pgpr. on the basis of 16s ribosomal rna sequences, 68 gram- ... | 2011 | 21876366 |
| the 184th residue of β-glucosidase bgl1b plays an important role in glucose tolerance. | bgl1b (acy09072) is a new gh1 family β-glucosidase derived from a marine microbial metagenomic library. in our previous study, the activity of recombinant bgl1b was competitively inhibited by glucose with an ic₅₀ value of 30 mm. based on amino acids sequence alignment with other gh1 β-glucosidases, including bglb from paenibacillus polymyxa whose structure has been solved, the 184th and 409th residues of bgl1b were suggested to be key residues relating to glucose tolerance or other properties. t ... | 2011 | 21856223 |
| Genome sequence of the diazotrophic Gram-positive rhizobacterium Paenibacillus riograndensis SBR5(T). | Paenibacillus riograndensis SBR5(T), a nitrogen-fixing Gram-positive rhizobacterium isolated from a wheat field in the south of Brazil, has a great potential for agricultural applications due to its plant growth promotion effects. Here we present the draft genome sequence of P. riograndensis SBR5(T). Its 7.37-Mb genome encodes determinants of the diazotrophic lifestyle and plant growth promotion, such as nitrogen fixation, antibiotic resistance, nitrate utilization, and iron uptake. | 2011 | 22038959 |
| the paenibacillus polymyxa species is abundant among hydrogen-producing facultative anaerobic bacteria in lake averno sediment. | lake averno sediment was used to isolate the facultative anaerobic bacteria having the potential for h(2) production. twenty-five out of 35 isolates recovered from the sediment sample produced hydrogen under anaerobic conditions from glucose with yields ranging from 0.1 to 0.49 mol h(2)/mol glucose. identification based on 16s rrna gene sequence analysis revealed that most of them belong to the firmicutes group, with a prevalence of the paenibacillus polymyxa species. seven distinct genomic fing ... | 2011 | 22038026 |
| isolation and functional gene analyses of aromatic-hydrocarbon-degrading bacteria from a polychlorinated-dioxin-dechlorinating process. | aerobic aromatic-hydrocarbon-degrading bacteria from a semi-anaerobic microbial microcosm that exhibited apparent complete dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (pcdd/fs) were isolated through enrichment and plating culture procedures with dibenzofuran as the model substrate. by 16s rrna gene sequence comparisons, these dibenzofuran-degrading isolates were identified as being members of the phyla actinobacteria, firmicutes, and proteobacteria, among which those of the ... | 2011 | 22146307 |
| antimicrobial activity of amazonian oils against paenibacillus species. | the gram-positive, spore-forming bacterium paenibacillus larvae is the primary bacterial pathogen of honeybee brood and the causative agent of american foulbrood disease (afb). one of the feasible alternative treatments being used for their control of this disease is essential oils. in this study in vitro antimicrobial activity of andiroba and copaíba essential oils against paenibacillus species, including p. larvae was evaluated. minimal inhibitory concentration (mic) in mueller-hinton broth by ... | 2011 | 22200645 |
| Colistin A and colistin B among inhibitory substances of Paenibacillus polymyxa JB05-01-1. | Recently, we isolated and reported the antagonism of Paenibacillus polymyxa JB05-01-1 (P. polymyxa JB05-01-1) against Gram-negative bacteria. Here, we provide more insights and attribute the abovementioned antagonism to the production of colistins A and B, which were purified by Amberlite column exchange, C18 column hydrophobicity, superdex 75 16/60 gel filtration chromatography connected to fast protein liquid chromatography and identified by MALDI TOF/TOF, and manual nanospray analysis. The am ... | 2011 | 22038095 |
| Updated genome assembly and annotation of Paenibacillus larvae, the agent of American foulbrood disease of honey bees. | As scientists continue to pursue various 'omics-based research, there is a need for high quality data for the most fundamental 'omics of all: genomics. The bacterium Paenibacillus larvae is the causative agent of the honey bee disease American foulbrood. If untreated, it can lead to the demise of an entire hive; the highly social nature of bees also leads to easy disease spread, between both individuals and colonies. Biologists have studied this organism since the early 1900s, and a century late ... | 2011 | 21923906 |
| cloning, expression, and characterization of a new xylanase from alkalophilic paenibacillus sp. 12-11. | a xylanase gene, xyn7c, was cloned from paenibacillus sp. 12-11, an alkalophilic strain isolated from the alkaline wastewater sludge of a paper mill, and expressed in escherichia coli. the full-length gene consists of 1,296 bp and encodes a mature protein of 400 residues (excluding the putative signal peptide) that belongs to the glycoside hydrolase family 10. the optimal ph of the purified recombinant xyn7c was found to be 8.0, and the enzyme had good ph adaptability at 6.5-8.5 and stability ov ... | 2011 | 21876378 |
| diversity and bioactive potential of endospore-forming bacteria cultured from the marine sponge haliclona simulans. | despite the frequent isolation of endospore-formers from marine sponges, little is known about the diversity and characterization of individual isolates. the main aims of this study were to isolate and characterize the spore-forming bacteria from the marine sponge haliclona simulans and to examine their potential as a source for bioactive compounds. | 2012 | 21985154 |
| Characterization of some efficient cellulase producing bacteria isolated from paper mill sludges and organic fertilizers. | The wide variety of bacteria in the environment permits screening for more efficient cellulases to help overcome current challenges in biofuel production. This study focuses on the isolation of efficient cellulase producing bacteria found in organic fertilizers and paper mill sludges which can be considered for use in large scale biorefining. Pure isolate cultures were screened for cellulase activity. Six isolates: S1, S2, S3, S4, E2, and E4, produced halos greater in diameter than the positive ... | 2011 | 21969070 |
| isolation and characterization of peptide antibiotics li-f04 and polymyxin b6 produced by paenibacillus polymyxa strain jsa-9. | paenibacillus polymyxa jsa-9 had been found to produce five cyclic li-f type antibiotics which were released into culture medium in accordance with our previous report. in this study, another three kinds of antagonistic compounds were extracted from p. polymyxa jsa-9 cell pellets and (or) spores by methanol. using high performance liquid chromatography (hplc) method, two antagonistic fractions were separated and collected from the methanol extract. one showed inhibition against escherichia coli ... | 2011 | 21864605 |