| engineering factor xa inhibitor with multiple platelet-binding sites facilitates its platelet targeting. | targeted delivery of antithrombotic drugs centralizes the effects in the thrombosis site and reduces the hemorrhage side effects in uninjured vessels. we have recently reported that the platelet-targeting factor xa (fxa) inhibitors, constructed by engineering one arg-gly-asp (rgd) motif into ancylostoma caninum anticoagulant peptide 5 (acap5), can reduce the risk of systemic bleeding than non-targeted acap5 in mouse arterial injury model. increasing the number of platelet-binding sites of fxa in ... | 2016 | 27432161 |
| quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel pma-qpcr methods. | in this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (pma-qpcr) to differentiate between viable and non-viable ancylostoma caninum ova. the newly developed method was validated using raw wastewater seeded with known numbers of a. caninum ova. results of this study confirmed that pma-qpcr has resulted in average of 88 % reduction (p < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. a reduction ... | 2016 | 27306209 |
| a hydroalcoholic extract from paullinia pinnata l. roots exerts anthelmintic activity against free-living and parasitic nematodes. | paullinia pinnata is a medicinal plant traditionally used in west africa against a wide range of diseases including soil-transmitted helminthiases. in this study, a hydroethanolic root extract was investigated for its phytochemical composition and in vitro activity against the free-living nematode caenorhabditis elegans as well as the larval stages of the parasitic helminths ancylostoma caninum, haemonchus contortus, toxocara cati, and trichuris vulpis.lc-ms analysis of the ethanol-water (1 : 1) ... | 2016 | 27286336 |
| standardization and application of the tetraprimer arms-pcr technique for screening of the e198a snp in the β-tubulin gene of hookworm populations in brazil. | the tetraprimer arms-pcr technique is efficient for snp detection and can be used to search for polymorphisms associated with drug resistance. however, the establishment of this methodology is not always straightforward because of the constraints on primer design due to the restrictions of the polymorphic regions. here, we describe the standardization of the tetraprimer arms-pcr methodology for the detection of a snp at codon 198 of the ancylostoma caninum β-tubulin gene. this snp is associated ... | 2016 | 27270392 |
| determination of ancylostoma caninum ova viability using metabolic profiling. | differentiation between viable and non-viable hookworm ova in environmental samples is necessary in order to implement strategies to mitigate re-infections in endemic regions. in this study, an untargeted metabolic profiling method was developed that utilised gas chromatography-mass spectrometry (gc-ms) in order to investigate hookworm ova viability. ancylostoma caninum was used to investigate the metabolites within viable and non-viable ova. univariate and multivariate statistical analyses of t ... | 2016 | 27236650 |
| is the infectiousness of dogs naturally infected with trypanosoma cruzi associated with poly-parasitism? | interactions among different species of parasites co-infecting the same host could be synergistic or antagonistic. these interactions may modify both the frequency of infected hosts and their infectiousness, and therefore impact on transmission dynamics. this study determined the infectiousness of trypanosoma cruzi-seropositive dogs (using xenodiagnosis) and their parasite load (quantified by qpcr), and tested the association between both variables and the presence of concomitant endoparasites. ... | 2016 | 27198799 |
| [cutaneous larva migrans: report of three cases from the western black sea region, turkey]. | cutaneous larva migrans (clm) is a parasitosis frequently seen in persons who have travelled to tropical or subtropical regions and in those who have worked in contact with soil. the disease frequently develops due to ancylostoma braziliensis and ancylostoma caninum species. after penetrating the skin and entering the body, the hookworm larva proceeds to bore tunnels through the epidermis, creating pruritic, erythematous, serpiginous lesions. secondary bacterial infections of the lesions can oft ... | 2016 | 27058342 |
| sensitivity and efficiency of selected coproscopical methods-sedimentation, combined zinc sulfate sedimentation-flotation, and mcmaster method. | coproscopical methods used in veterinary-parasitological diagnostics were validated according to their sensitivity (se) and egg recovery rate [efficiency (ef)]. validation of the combined sedimentation-flotation method and the modified mcmaster method was performed by using feces spiked with eggs of ancylostoma caninum, uncinaria stenocephala, cooperia oncophora, cyathostomins, ascaris suum, toxascaris leonina, toxocara canis, trichuris vulpis, moniezia expansa, and anoplocephala perfoliata. for ... | 2016 | 26997342 |
| new hosts and localities for helminths of carnivores in argentina. | a total of 111 samples (43 faeces and 79 gastrointestinal tracts) of 14 wild carnivore species from 12 argentine provinces were analyzed. helminth eggs were identified in 73% of the faecal samples and adult worms were recovered from 81% of the gastrointestinal tracts. we found 19 helminth species. among the most frequent findings were parasites of domestic carnivores, namely toxocara canis, toxocara cati, toxascaris leonina, ancylostoma caninum, ancylostoma tubaeforme and uncinaria stenocephala. ... | 2015 | 26701468 |
| larva migrans in india: veterinary and public health perspectives. | despite an important public health problem in developing world like india, larva migrans remains a neglected zoonosis. cutaneous larva migrans, visceral larva migrans, and ocular larva migrans are the important clinical manifestations seen in humans in india. although many nematode parasites have the ability to cause the infection, the disease primarily occurs due to ancylostoma caninum and toxocara canis. presence of the infection in dogs is an indirect indication of its incidence in humans in ... | 2015 | 26688621 |
| detection of helminth eggs and identification of hookworm species in stray cats, dogs and soil from klang valley, malaysia. | the present study was conducted to determine the prevalence of helminth eggs excreted in the faeces of stray cats, dogs and in soil samples. a total of 505 fresh samples of faeces (from 227 dogs and 152 cats) and soil were collected. the egg stage was detected via microscopy after the application of formalin-ether concentration technique. genomic dna was extracted from the samples containing hookworm eggs and used for further identification to the species level using real-time polymerase chain r ... | 2015 | 26671680 |
| prevalence of gastrointestinal parasites in domestic dogs in tabasco, southeastern mexico. | the overall goal of this study was to estimate the prevalence of gastrointestinal (gi) parasites in dogs in the city of villahermosa in tabasco, mexico. the study population consisted of 302 owned dogs that had limited access to public areas. a fecal sample was collected from each animal and examined for gi parasites by conventional macroscopic analysis and centrifugal flotation. fecal samples from 80 (26.5%) dogs contained gi parasites. of these, 58 (19.2%) were positive for helminths and 22 (7 ... | 2015 | 26648011 |
| wild red foxes (vulpes vulpes) as sentinels of parasitic diseases in the province of soria, northern spain. | four hundred red foxes (vulpes vulpes) were examined for ecto- (arthropods) and endoparasites (leishmania spp., trichinella spp., and intestinal parasites). different species of flea (total prevalence, 40.50%), tick (16.25%), mite (7.25%), and fly (1.50%) were identified. the most prevalent flea was pulex irritans (found on 29% of the foxes); the most prevalent tick, mite, and fly were ixodes canisuga (on 5%), sarcoptes scabiei (on 5.25%), and hippobosca equina (on 1%), respectively. the endopar ... | 2015 | 26565688 |
| comparison of concentration methods for rapid detection of hookworm ova in wastewater matrices using quantitative pcr. | hookworm infection contributes around 700 million infections worldwide especially in developing nations due to increased use of wastewater for crop production. the effective recovery of hookworm ova from wastewater matrices is difficult due to their low concentrations and heterogeneous distribution. in this study, we compared the recovery rates of (i) four rapid hookworm ova concentration methods from municipal wastewater, and (ii) two concentration methods from sludge samples. ancylostoma canin ... | 2015 | 26358269 |
| prevalence of endoparasitic infections of non descript dogs in mathura, uttar pradesh. | the objective of this study was to investigate, through cross-sectional survey, the distribution, types and prevalence of gastro-intestinal parasites affecting stray dogs in and around mathura, india. freshly voided faecal samples of 108 identified, stray, non descript dogs of either sex and different age groups were collected and examined for coprological examination by direct faecal smear method; simple flotation and sedimentation techniques to detect parasitic oocysts and/or eggs. a total of ... | 2015 | 26345058 |
| apparent contact dermatitis caused by ancylostoma caninum: a case report. | ancylostomum caninum larvae cause damage to the host at the point of entry through the skin leaving a wound vulnerable to secondary infections. as the larvae migrate through the skin an inflammatory response, dermatitis, is often stimulated which can be exacerbated in hosts which give hypersensitive responses. we assessed a 44-year-old man with contact dermatitis diagnosed as nickel allergy but caused by ancylostoma caninum infection. | 2015 | 26342510 |
| rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time pcr method. | the risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. a rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. in this study, a real-time pcr method was developed for the rapid, sensitive and specific detection of canine ... | 2015 | 26297680 |
| expression profile of heat shock response factors during hookworm larval activation and parasitic development. | when organisms are exposed to an increase in temperature, they undergo a heat shock response (hsr) regulated by the transcription factor heat shock factor 1 (hsf-1). the heat shock response includes the rapid changes in gene expression initiated by binding of hsf-1 to response elements in the promoters of heat shock genes. heat shock proteins function as molecular chaperones to protect proteins during periods of elevated temperature and other stress. during infection, hookworm infective third st ... | 2015 | 26296769 |
| mucosal tolerance of the hookworm ancylostoma caninum in the gut of naturally infected wild dogs. | ancylostoma caninum is a very pathogenic hookworm that locates in the small intestine of the dog and other canid species. the mucosal response of wild dogs naturally infected with a. caninum was investigated in this study. in spite of diffuse infiltrations of the mucosa with cd3(+) , cd4(+) , cd8(+) , cd11c(+) , cd21(+) or mhc class ii antigen cells no focal infiltrations with any of these cell phenotypes were observed around the buccal capsule or the body of the feeding worms. very few or no ap ... | 2015 | 26216244 |
| a recombinant fusion protein derived from dog hookworm inhibits autoantibody-induced dermal-epidermal separation ex vivo. | the proteins secreted by parasitic nematodes are evolutionarily optimized molecules with unique capabilities of suppressing the immune response of the host organism. neutrophil inhibitory factor (nif), which is secreted by the dog hookworm ancylostoma caninum, binds to the β2 integrin cd11b/cd18, which is expressed on human neutrophils, eosinophils, monocytes and macrophages and inhibits neutrophil-dependent lung injury and neutrophil invasion of ischaemic brain tissue. neutrophils are key playe ... | 2015 | 26174039 |
| development of a new amplification-refractory mutation system for detection of a single nucleotide polymorphism linked to drug resistance in ancylostoma caninum. | single nucleotide polymorphisms at codons 167, 198, and 200 in the β-tubulin isotype 1 gene have been associated with benz-imidazole resistance. until now, the only mutation observed in ancy-lostoma caninum was at codon 200 of this gene. however, the standard-ized methodologies used to detect mutations in this species are faulty. the objective of this study was to standardize a molecular technique based on amplification-refractory mutation system-polymerase chain reaction (arms-pcr) for detectin ... | 2015 | 26125702 |
| an epidemiological survey on intestinal helminths of stray dogs in mashhad, north-east of iran. | this research was conducted to determine the prevalence of gastrointestinal helminths in stray dogs in the northeast of iran, with special attention to those parasites that can be transmitted to human. in this experiment, a total of 72 adult and 18 juvenile stray dogs were collected and necropsied for the presence of helminth parasites from october 2011 to august 2012. the overall prevalence of gastrointestinal helminths was 86 % (95 % ci: 79.2-92.8 %). the observed helminths of the gastrointest ... | 2015 | 26064015 |
| the structure of hookworm platelet inhibitor (hpi), a cap superfamily member from ancylostoma caninum. | secreted protein components of hookworm species include a number of representatives of the cysteine-rich/antigen 5/pathogenesis-related 1 (cap) protein family known as ancylostoma-secreted proteins (asps). some of these have been considered as candidate antigens for the development of vaccines against hookworms. the functions of most cap superfamily members are poorly understood, but one form, the hookworm platelet inhibitor (hpi), has been isolated as a putative antagonist of the platelet integ ... | 2015 | 26057788 |
| new tools for ntd vaccines: a case study of quality control assays for product development of the human hookworm vaccine na-apr-1m74. | na-apr-1(m74) is an aspartic protease that is rendered enzymatically inactive by site-directed mutagenesis and is a candidate antigen component in the human hookworm vaccine. the mutant protease exerts vaccine efficacy by inducing antibodies that neutralize the enzymatic activity of wild type enzyme (na-apr-1wt) in the gut of the hookworm, thereby depriving the worm of its ability to digest its blood meal. previously, canines immunized with na-apr-1(m74) and challenged with ancylostoma caninum w ... | 2015 | 26018444 |
| differences in the faecal microbiome of non-diarrhoeic clinically healthy dogs and cats associated with giardia duodenalis infection: impact of hookworms and coccidia. | the protozoan parasite giardia duodenalis causes a waterborne diarrhoeal disease in animals and humans, yet many giardia-infected hosts remain asymptomatic. mixed parasite infections are common in both animals and humans with unknown consequences for giardia or other parasites. we compared the composition and diversity of bacterial communities from 40 dogs, including free-roaming dogs, and 21 surrendered cats from australia. the dog cohort included 17 (42.5%) dogs positive for giardia and 13 (32 ... | 2015 | 25934152 |
| structure-guided creation of acap5-derived and platelet targeted factor xa inhibitors. | anticoagulants and anti-platelet agents are simultaneously administrated in clinical practice (i.e. percutaneous coronary intervention), which cause significant risk of systemic bleeding. targeted delivery of anticoagulants to the activated platelets at sites of vascular injuries may condense the site-specific anticoagulant effect and reduce the hemorrhage side effects in uninjured vessels. to this end, we prepared three ancylostoma caninum anticoagulant peptide 5 (acap5) variants nr1, nr2 and n ... | 2015 | 25887920 |
| molecular analysis of the f167y snp in the β-tubulin gene by screening genotypes of two ancylostoma caninum populations. | mutations in the β-tubulin isotype 1 gene at codons 167 (f167y), 198 (e198a) and 200 (f200y) have been associated with benzimidazole resistance in helminths. the f200y polymorphism has previously been described for ancylostom caninum; however, the f167y polymorphism has not been investigated in members of the ancylostomatidae family. the aim of this study was to screen for the f167y polymorphism in a. caninum isolates recovered from naturally infected dogs in two brazilian states. no mutation wa ... | 2015 | 25865406 |
| zoonotic parasites in feces and fur of stray and private dogs from italy. | the present study evaluated the occurrence of zoonotic parasites in feces and on fur of stray and private dogs living in italy. individual fecal samples collected from 117 and 385 kenneled (i.e., rescue shelter) and privately owned dogs, respectively, were examined by conventional copromicroscopy. samples positive for giardia duodenalis and cryptosporidium spp. were subjected to further genetic characterization. additionally, 174 fur samples of private and kenneled dogs living in the same area, ... | 2015 | 25773179 |
| spatial distribution of canine zoonotic enteroparasites in bahía blanca, argentina. | the objectives of this research were: (1) to determine the occurrence of zoonotic enteroparasites in dog feces from bahía blanca, argentina; (2) to characterize the spatial distribution of the parasites found in association with the quality of life index (qli) in neighborhoods of bahía blanca; and (3) to determine if the presence of a particular parasite genus in a stool sample was facilitated or impeded by the presence of other parasite genera. samples of dog stools (n=475) were collected betwe ... | 2015 | 25705047 |
| recombinant nematode anticoagulant protein c2 inhibits cell invasion by decreasing upa expression in nsclc cells. | nematode anticoagulant protein c2 (napc2) is an 85-residue polypeptide originally isolated from the hematophagous hookworm, ancylostoma caninum. several studies have shown that rnapc2 inhibits the growth of primary and metastatic tumors in mice independently of its ability to initiate coagulation. we obtained bioactive recombinant rnapc2 by splicing of the rnapc2-intein-cbd fusion proteins expressed in e. coli er2566. in the in vitro assay, rnapc2 obviously inhibited the invasive ability of non- ... | 2015 | 25672417 |
| prevalence of intestinal parasites in breeding kennel dogs in japan. | the present study is the first to show overall prevalences of intestinal parasites among breeding kennel dogs in japan. a total of 573 fresh fecal samples were collected from dogs at 12 breeding kennels. giardia-specific coproantigen was examined by elisa kit (snap(®) giardia, idexx laboratories, inc., maine, usa). other intestinal parasites were determined microscopically using the formalin-ethyl acetate sedimentation technique. overall prevalences of two genera of protists, giardia spp. and cy ... | 2015 | 25627028 |
| [potential hazard of zoonotic parasites present in canine feces in puerto escondido, oaxaca]. | to estimate the zoonotic parasites prevalence in feral dog feces in puerto escondido. | 2017 | 25604414 |
| two potential hookworm daf-16 target genes, snr-3 and lpp-1: gene structure, expression profile, and implications of a cis-regulatory element in the regulation of gene expression. | hookworms infect nearly 700 million people, causing anemia and developmental stunting in heavy infections. little is known about the genomic structure or gene regulation in hookworms, although recent publication of draft genome assemblies has allowed the first investigations of these topics to be undertaken. the transcription factor daf-16 mediates multiple developmental pathways in the free living nematode caenorhabditis elegans, and is involved in the recovery from the developmentally arrested ... | 2015 | 25573064 |
| first identification of the f200y snp in the β-tubulin gene linked to benzimidazole resistance in ancylostoma caninum. | single nucleotide polymorphisms (snps) in the β-tubulin isotype 1 gene in codons 167, 198 and 200 have been associated with benzimidazole resistance in some nematodes, although no polymorphisms in this gene have been described in the hookworm ancylostoma caninum to date. this study aimed to screen for snps in the β-tubulin isotype 1 gene at codons 198 and 200 associated with benzimidazole resistance in a. caninum worms recovered from naturally infected dogs from two brazilian states (minas gerai ... | 2014 | 25468029 |
| field evaluation of the efficacy and safety of a combination of spinosad and milbemycin oxime in the treatment and prevention of naturally acquired flea infestations and treatment of intestinal nematode infections in dogs in europe. | two separate randomised, blinded, multicentre field trials were conducted to evaluate the efficacy and safety of a combination of spinosad and milbemycin oxime (mo) (trifexis(®), elanco animal health) in the treatment and prevention of naturally acquired flea infestations and intestinal nematode infections in european dogs. treatments using trifexis(®) and each control veterinary product (cvp) were administered once on day 0 in both field studies. in the flea field trial, 11 veterinary clinics i ... | 2015 | 25465736 |
| the prevalence and distribution of gastrointestinal parasites of stray and refuge dogs in four locations in india. | a gastrointestinal parasite survey of 411 stray and refuge dogs sampled from four geographical and climactically distinct locations in india revealed these animals to represent a significant source of environmental contamination for parasites that pose a zoonotic risk to the public. hookworms were the most commonly identified parasite in dogs in sikkim (71.3%), mumbai (48.8%) and delhi (39.1%). in ladakh, which experiences harsh extremes in climate, a competitive advantage was observed for paras ... | 2014 | 25139393 |
| identification of angiostrongylus cantonensis and other nematodes using the ssu rdna in achatina fulica populations of metro manila. | angiostrongylus cantonensis is a parasitic nematode that causes eosinophilic meningitis in humans. accidental infection occurs by consumption of contaminated intermediates, such as the giant african land snail, achatina fulica. this study surveyed the presence of a. cantonensis juveniles in a. fulica populations from 12 sites in metropolitan manila, philippines using the ssu rdna. fourteen distinct sequences from 226 nematodes were obtained; of these, two matched a. cantonensis and ancylostoma c ... | 2014 | 25134902 |
| efficacy of milbemycin oxime in combination with spinosad in the treatment of larval and immature adult stages of ancylostoma caninum and toxocara canis in experimentally infected dogs. | ancylostoma caninum and toxocara canis are two important zoonotic parasites of dogs. the primary objective of these studies were to confirm the oral effectiveness of milbemycin oxime (mo) and spinosad in dogs experimentally infected with immature (l4 and immature adult) stages of t. canis or a. caninum. both trials were conducted as randomized, blinded, placebo-controlled dose confirmation studies. treatments using the intended european commercial tablet formulation of trifexis were administered ... | 2014 | 25108851 |
| assessing the speed of kill of hookworms, ancylostoma caninum, by advantage multi ® for dogs using endoscopic methods. | endoscopic capsules and endoscopy were used to assess the speed of kill and the clearance of hookworms in dogs experimentally infected with ancylostoma caninum. a total of four adult dogs were inoculated in two separate cohorts comprised of two 4-year-old females and two 7-year-old males. dogs were treated topically with advantage multi(®) for dogs 13 days (cohort 1) or 16 days (cohort 2) after infection. endoscopic imaging of the small intestine was carried out both pre- and post-treatment. exa ... | 2014 | 24953752 |
| intestinal helminths of golden jackals and red foxes from tunisia. | forty wild canids including 31 golden jackals (canis aureus linné, 1758) and 9 red foxes (vulpes vulpes linné, 1758) collected between 2008 and 2011 in the northeast, northwest and center of tunisia were necropsied and examined for intestinal helminth parasites. all jackals and foxes were found infected with a prevalence rate of 95% for cestodes, 82.5% for nematodes and 7.5% for acanthocephalans. a total of twelve helminth species were recorded in red foxes: cestodes, dipylidium caninum (55.6%), ... | 2014 | 24938826 |
| kv1.3 channel-blocking immunomodulatory peptides from parasitic worms: implications for autoimmune diseases. | the voltage-gated potassium (kv) 1.3 channel is widely regarded as a therapeutic target for immunomodulation in autoimmune diseases. shk-186, a selective inhibitor of kv1.3 channels, ameliorates autoimmune diseases in rodent models, and human phase 1 trials of this agent in healthy volunteers have been completed. in this study, we identified and characterized a large family of stichodactyla helianthus toxin (shk)-related peptides in parasitic worms. based on phylogenetic analysis, 2 worm peptide ... | 2014 | 24891519 |
| cloning and expression of apyrase gene from ancylostoma caninum in escherechia coli. | apyrase encoding metal-ions activated plasma membrane protease is present in animal and plant tissues. this enzyme can hydrolyze adp and atp pyrophosphate bond, resulting in amp and free phosphate groups, and plays an important role for insects and parasites to evade host immune system. however localization and function of apyrase in the canine hookworm, ancylostoma caninum, remains unknown. to analyze apyrase gene in a. caninum (a eukaryotic parasitic hookworm), a pair of primers was designed a ... | 2014 | 26204021 |
| evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis. | angiostrongylus vasorum is a potentially fatal canine nematode. due to the high variability of clinical signs and the often chronic and subtle course of the infections, the diagnosis is particularly challenging. a rapid in-clinic assay (angio detect test, idexx laboratories, westbrook, maine, usa) for the serological detection of circulating antigen and intended for routine in-clinic diagnosis has been evaluated. | 2014 | 24548539 |
| molecular detection of ancylostoma duodenale, ancylostoma ceylanicum, and necator americanus in humans in northeastern and southern thailand. | the 2 principal species of hookworms infecting humans are necator americanus and ancylostoma duodenale. case studies on zoonotic hookworm infections with ancylostoma ceylanicum and/or ancylostoma caninum are known mainly from asian countries. of these 2 zoonotic species, only a. ceylanicum can develop to adulthood in humans. in the present study, we report a molecular-based survey of human hookworm infections present in southern and northeastern thailand. thirty larval hookworm samples were obta ... | 2013 | 24516284 |
| parasitological and hematological aspects of co-infection with angiostrongylus vasorum and ancylostoma caninum in dogs. | dogs are frequently infected by one or more species of endoparasites, which can lead to secondary infections that cause high morbidity and death. in this context, 2 nematode species are of veterinary importance: angiostrongylus vasorum, which is a parasite of the pulmonary artery and right ventricle in domestic and wild canids, and ancylostoma caninum, which is a parasite of the small intestine in felids and domestic and wild canids. we used 20 mongrel dogs that were divided into groups and infe ... | 2014 | 24373514 |
| engineering novel anticoagulant proteins by motif grafting. | structure-based rational design has been considered as a promising approach to design novel proteins. for this purpose, we designed artificial anticoagulant proteins that are able to target factor xa (fxa) using a functional motifgrafting approach. the motif corresponded to the residues cys15 to cys42 of ancylostoma caninum anticoagulant peptide 5 (acap5), a potent fxa inhibitor. by screening of the protein data bank (pdb) using vector alignment search tool (vast, search for three-dimensional sc ... | 2014 | 24370339 |
| molecular identification of ancylostoma caninum isolated from cats in southern china based on complete its sequence. | ancylostoma caninum is a blood-feeding parasitic intestinal nematode which infects dogs, cats, and other mammals throughout the world. a highly sensitive and species-specific pcr-rflp technique was utilised to detect the prevalence of a. caninum in cats in guangzhou, southern china. of the 102 fecal samples examined, the prevalence of a. caninum in cats was 95.1% and 83.3% using pcr-rflp and microscopy, respectively. among them, the prevalence of single hookworm infection with a. caninum was 54. ... | 2013 | 24175305 |
| ancylostoma ceylanicum, a re-emerging but neglected parasitic zoonosis. | although ancylostoma ceylanicum is known to be an endemic and widely distributed hookworm of dogs and cats in asia, its contribution to human morbidity as a potentially zoonotic hookworm remains largely unexplored. since its discovery by lane (1913) as a 'new parasite' of humans a century ago, the hookworm has been regarded as a 'rare' and 'abnormal' parasite and largely overlooked in surveys of human parasites. recent molecular-based surveys in asia, however, have demonstrated that a. ceylanicu ... | 2013 | 23968813 |
| [prevalence of echinococcus granulosus determined with polymerase chain reaction in dogs in aydın district]. | in this study, it was aimed to determine the prevalence of e. granulosus in the aydın district. | 2013 | 23955903 |
| a coprological survey of intestinal helminthes in stray dogs captured in osaka prefecture, japan. | this study aimed to investigate intestinal helminth infection in stray dogs in osaka prefecture by surveying coprological samples from dogs captured from 2006-2011. of 212 fecal samples collected, overall prevalence of infection was 39.2%. the most common species was toxocara canis (25.0%), followed by trichuris vulpis (8.0%), spirometra erinaceieuropaei (3.3%), taeniidae (2.4%), ancylostoma caninum (1.9%) and toxascaris leonine (0.5%). in the molecular analysis, all of the taeniid eggs were neg ... | 2013 | 23774027 |
| efficacy of emodepside plus toltrazuril oral suspension for dogs (procox®, bayer) against trichuris vulpis in naturally infected dogs. | the efficacy of emodepside plus toltrazuril oral suspension for dogs (procox®, bayer) against trichuris vulpis was evaluated in a controlled, blinded and randomised laboratory study. twenty naturally infected dogs were included. dogs in the treatment group received the minimum therapeutic dose of 0.45 mg emodepside and 9 mg toltrazuril per kg body weight, while dogs in the control group were left untreated. efficacy was calculated based on worm counts after necropsy on day 7 post treatment. addi ... | 2013 | 23756961 |
| a sensitive and specific pcr assay for the detection of baylisascaris schroederi eggs in giant panda feces. | baylisascaris schroederi is one of the most common intestinal nematodes in giant pandas. it can cause severe baylisascariasis which is highly infectious in its natural hosts. a rapid and reliable diagnosis of parasite infections is crucial to protect giant pandas, as well as for environmental monitoring and disease surveillance. here, we established a specific pcr assay for b. schroederi detection which was targeting a 331-bp long fragment of the mitochondrial cytochrome c oxidase subunit ii (co ... | 2013 | 23688803 |
| comparison of ancylostoma caninum worm counts acquired by endoscopy and necropsy. | many regulatory agencies require that the efficacy of veterinary anthelmintic medications be evaluated by enumerating parasites in treated and untreated animals after necropsy. current ethical considerations, i.e., the 3 rs of research, call for the replacement of this method with less invasive techniques that would not require animal sacrifice. this study tested standard gastrointestinal endoscopy as an in vivo method of quantifying the intestinal hookworm, ancylostoma caninum. worm counts were ... | 2013 | 23601667 |
| hookworm excretory/secretory products induce interleukin-4 (il-4)+ il-10+ cd4+ t cell responses and suppress pathology in a mouse model of colitis. | evidence from human studies and mouse models shows that infection with parasitic helminths has a suppressive effect on the pathogenesis of some inflammatory diseases. recently, we and others have shown that some of the suppressive effects of hookworms reside in their excretory/secretory (es) products. here, we demonstrate that es products of the hookworm ancylostoma caninum (aces) suppress intestinal pathology in a model of chemically induced colitis. this suppression was associated with potent ... | 2013 | 23545299 |
| molecular and biological diagnostic tests for monitoring benzimidazole resistance in human soil-transmitted helminths. | in endemic countries with soil-transmitted helminths mass drug administration with albendazole or mebendazole are being implemented as a control strategy. however, it is well known in veterinary helminths that the use of the same benzimidazole drugs can place selection on the β-tubulin gene, leading to resistance. given the concern that resistance could arise in human soil-transmitted helminths, there is an urgent need to develop accurate diagnostic tools for monitoring resistance. in this study ... | 2013 | 23458960 |
| detailed transcriptome description of the neglected cestode taenia multiceps. | the larval stage of taenia multiceps, a global cestode, encysts in the central nervous system (cns) of sheep and other livestock. this frequently leads to their death and huge socioeconomic losses, especially in developing countries. this parasite can also cause zoonotic infections in humans, but has been largely neglected due to a lack of diagnostic techniques and studies. recent developments in next-generation sequencing provide an opportunity to explore the transcriptome of t. multiceps. | 2012 | 23049872 |
| cloning, sequencing and phylogenetic analysis of the small gtpase gene cdc-42 from ancylostoma caninum. | cdc-42 is a member of the rho gtpase subfamily that is involved in many signaling pathways, including mitosis, cell polarity, cell migration and cytoskeleton remodeling. here, we present the first characterization of a full-length cdna encoding the small gtpase cdc-42, designated as accdc-42, isolated from the parasitic nematode ancylostoma caninum. the encoded protein contains 191 amino acid residues with a predicted molecular weight of 21 kda and displays a high level of identity with the rho- ... | 2012 | 23000556 |
| high prevalence of intestinal infections and ectoparasites in dogs, minas gerais state (southeast brazil). | in the present study, 155 dogs euthanized by the zoonotic disease unit of uberlândia in minas gerais state (southeast brazil) were autopsied. ectoparasites were collected, and the intestinal content of dogs was systematically examined for the presence of helminthic parasites. in total, we isolated 5,155 metazoan parasites of eight species (three intestinal helminth species, five ectoparasite species). the cestode dipylidium caninum was present in 57 dogs (36.8 %), the nematodes ancylostoma canin ... | 2012 | 22825284 |
| sensitivity of double centrifugation sugar fecal flotation for detecting intestinal helminths in coyotes (canis latrans). | fecal analysis is commonly used to estimate prevalence and intensity of intestinal helminths in wild carnivores, but few studies have assessed the reliability of fecal flotation compared to analysis of intestinal tracts. we investigated sensitivity of the double centrifugation sugar fecal flotation and kappa agreement between fecal flotation and postmortem examination of intestines for helminths of coyotes (canis latrans). we analyzed 57 coyote carcasses that were collected between october 2010 ... | 2012 | 22740537 |
| prevalence and zoonotic potential of canine hookworms in malaysia. | canine hookworm infection is endemic in southeast asian countries with a prevalence ranging from 70% to 100%, with zoonotic transmission representing a potentially significant public health concern. however, there are limited data available on the prevalence of canine hookworms in malaysia. this study was conducted to determine the prevalence of hookworm and ancylostoma species among dogs in malaysia. | 2012 | 22564445 |
| serine protease inhibitors of parasitic helminths. | serine protease inhibitors (serpins) are a superfamily of structurally conserved proteins that inhibit serine proteases and play key physiological roles in numerous biological systems such as blood coagulation, complement activation and inflammation. a number of serpins have now been identified in parasitic helminths with putative involvement in immune regulation and in parasite survival through interference with the host immune response. this review describes the serpins and smapins (small seri ... | 2012 | 22310379 |
| [a few peculiar verminal infections in indonesia: plagiorchis javensis; ancylostoma caninum; meyers-kouwenaar syndrome, hypereosinophilia and microfilariasis in the lymph glands]. | | 1950 | 24538151 |
| road killed carnivores illustrate the status of zoonotic helminthes in caspian sea littoral of iran. | carnivore carcasses on the roads can be regarded as study materials in parasitology and eco-epidemiology. stray carnivores such as dogs and cats are known to harbor so many different pathogens like zoonotic helminthes. the current investigation, apparent the status of the helminthic parasites found in road killed carnivores from different parts of guilan province north of iran. | 2017 | 28761483 |
| prevalence and risk factors associated with endoparasitic infection in dogs from transylvania (romania): a retrospective study. | during six years (april 2010-april 2016) we examined individual feces samples collected from 1314 dogs located in center and northwest romania (transylvania). stool samples were analyzed by saturated salt flotation, sedimentation technique and modified ziehl-nielsen staining method. the overall prevalence of endoparasitic infections was 66.6% (n=875). sixteen species/genera of endoparasites were identified. the most prevalent species were ancylostoma caninum/uncinaria stenocephala (33.0%) (p=0.0 ... | 2017 | 28807286 |
| quantification of hookworm ova from wastewater matrices using quantitative pcr. | a quantitative pcr (qpcr) assay was used to quantify ancylostoma caninum ova in wastewater and sludge samples. we estimated the average gene copy numbers for a single ovum using a mixed population of ova. the average gene copy numbers derived from the mixed population were used to estimate numbers of hookworm ova in a. caninum seeded and unseeded wastewater and sludge samples. the newly developed qpcr assay estimated an average of 3.7×10(3) gene copies per ovum, which was then validated by seedi ... | 2017 | 28647243 |
| comparison of culture-based, vital stain and pma-qpcr methods for the quantitative detection of viable hookworm ova. | accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. in this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (pma-qpcr)] was evaluated by enumerating 1,000 ± 50 ancylostoma caninum ova in the laboratory. the culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. ... | 2017 | 28617281 |
| morphometric and scanning electron microscopy based identification of ancylostoma caninum parasites in dog. | ancylostoma caninum, a blood feeding nematode parasite (family: ancylostomatidae, superfamily: ancylostomatoidea) can cause anaemia, dark reddish-brown to black haemorrhagic diarrhoea, dehydration, wasting and deaths due to heavy blood loss. adult hook worm parasites recovered from the intestine of a stray dog at the time of necropsy were identified as a. caninum based on morphological characters and morphometric observations involving scanning electron microscopy (sem). different developmental ... | 2017 | 28615871 |
| enzyme-linked immunosorbent assays for coproantigen detection of ancylostoma caninum and toxocara canis in dogs and toxocara cati in cats. | we report the development and field validation of 2 elisas for the detection of ancylostoma caninum or toxocara canis coproantigens in the feces of dogs with experimental and natural infections, and evidence of cross-reactivity with respective feline counterparts. a. caninum-specific coproantigens were detected in feces of experimentally infected dogs starting at 9 d post-infection (dpi), whereas eggs were not seen until 23 dpi. t. canis-specific coproantigens were detected in 3 of 5 experimenta ... | 2017 | 28424002 |