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human external ophthalmomyiasis caused by lucilia sericata meigen (diptera: calliphoridae)--a green bottle fly.ophthalmomyiasis externa is the result of infestation of the conjunctiva by the larval form or maggots of flies from the order diptera. if not recognized and managed appropriately, it can be complicated by the potentially fatal condition ophthalmomyiasis interna. ophthalmomyiasis externa is mainly caused by the sheep bot fly (oestrus ovis). we present the first case, to our knowledge, of ophthalmomyiasis externa in an elderly woman from belgrade caused by lucilia sericata meigen--a green bottle ...201425022307
nosocomial myiasis with lucilia sericata (diptera: calliphoridae) in an icu patient in mashhad, northeastern of iran.myiasis is the invasion of larvae to human or animal live tissues by flies belonging to the order diptera and families like calliphoridae, sarcophagidae, oesteridae, etc. although rare, nosocomial myiasis must be noted carefully, especially in case of hospitalized patients. a 63-year old man admitted to an icu ward in mashhad is investigated and presented in this research. on the 35(th) day of hospitalization, about 100 larvae 6-7 mm in length, yellow to cream and fusiform were observed around t ...201424979568
application of two-way hierarchical cluster analysis for the identification of similarities between the individual lipid fractions of lucilia sericata.the composition of the cuticular and internal lipids of larvae and pupae of lucilia sericata was studied using chromatographic techniques. the lipids from both stages of l. sericata had similar free fatty acid (ffa) profiles and also contained alcohols and cholesterol. the range of the number of c-atoms detected for these classes of compounds was to some extent similar in larvae and pupae, but the relative amounts of each class differed between stages. saturated as well as unsaturated ffas with ...201424827683
[effect of feeding on different tissues on larva development of lucilia sericata].to observe the effect of feeding on different pig tissues on the development of lucilia sericata larvae.201324809193
could microbe stimulated maggots become a targeted natural antibiotics family?maggot debridement therapy plays an important role in treatment of diabetic foot ulcers and other chronic infectious wounds, cause of this is its extremely low drug resistance. however, the microbe stimulated maggot, we may call it a derivative of normal sterile maggot, could exhibit stronger bacterial or bactericidal effects. methods of the pretreatment on maggot was different germ solution were artificially mixed and added with originally sterile maggots, the novel secretions were collected. s ...201424793131
cryopreservation of embryos of lucilia sericata (diptera: calliphoridae).embryos of lucilia (phaenicia) sericata (meigen) (diptera calliphoridae), the green blowfly, were successfully cryopreserved by vitrification in liquid nitrogen and stored for 8 yr. embryos incubated at 19 degrees c for 17 h after oviposition were found to be the most appropriate stage to cryopreserve. removal of the embryonic surface water was done using 2-propanol before the alkane treatment to permeabilize the embryo. exposure to 2-propanol for > 10 s caused necrotic tissue damage in the embr ...201424724284
a novel serine protease secreted by medicinal maggots enhances plasminogen activator-induced fibrinolysis.maggots of the blowfly lucilia sericata are used for the treatment of chronic wounds. as haemostatic processes play an important role in wound healing, this study focused on the effects of maggot secretions on coagulation and fibrinolysis. the results showed that maggot secretions enhance plasminogen activator-induced formation of plasmin and fibrinolysis in a dose- and time-dependent manner. by contrast, coagulation was not affected by secretions. biochemical studies indicated that a novel seri ...201424647546
oral cavity myiasis in children: first demonstration in egypt with general review.myiasis is a problem of medical veterinary and real economic importance which affects the human welfare worldwide particularly in animal raising countries. myiasis of man and animals is a real welfare problem of worldwide distribution. the most important insects from medical point of view are the blood suckers or the insect-borne diseases. still others, almost non-blood suckers, but may attack man and animal to deposit their eggs or larvae causing pathogenic conditions in skin, nose, eye, lung, ...201324640873
field study on the attraction and development of insects on human meconium and breast-fed-infant feces.urogenital myiasis of newborn infants, although rare, is usually considered to indicate neglect due to attraction of flies to feces; however, infant feces have not been determined to attract insects. human meconium and breast-fed-infant feces were used to determine attractiveness to insects and to examine subsequent colonization and growth patterns of insect larvae. despite small amounts of fecal material present, adults of lucilia sericata arrived at breast-fed-infant feces within five minutes; ...201424635042
lucimycin, an antifungal peptide from the therapeutic maggot of the common green bottle fly lucilia sericata.we report the identification, cloning, heterologous expression and functional characterization of a novel antifungal peptide named lucimycin from the common green bottle fly lucilia sericata. the lucimycin cdna was isolated from a library of genes induced during the innate immune response in l. sericata larvae, which are used as therapeutic maggots. the peptide comprises 77 amino acid residues with a molecular mass of 8.2 kda and a pi of 6.6. it is predicted to contain a zinc-binding motif and t ...201424622788
quantifying the temperature of maggot masses and its relationship to decomposition.numerous calliphoridae species have been observed to form larval aggregations during the feeding stage of development, resulting in localized increases in temperature. this study investigates the relationship between maggot numbers in a mass and heat generation. single-species aggregations (lucilia sericata) of various sizes (50-2500 individuals) were reared in the laboratory at a constant ambient temperature of 22°c. internal mass temperatures were recorded every 5 min throughout the feeding st ...201424502332
the analysis of pupal development period in lucilia sericata (diptera: calliphoridae) forensically important insect.forensic entomology is a study that insect evidence is used for the determination of postmortem interval (pmimin). while determining pmimin, the pupal period is given as the duration between the beginning of the pupal period and the stage of adult emergence. the pupal period constitutes approximately 50 % of the immature development, and with detailed examination it would be possible to present a much more accurate pmimin. in this study, the pupal period of lucilia sericata (meigen, 1826), which ...201424477452
[a woman with a painful toe].a 64-year-old woman visited the surgeon because of an infected right hallux. after investigation we concluded she had myiasis caused by an infestation with lucilia sericata. this usually occurs in tropical regions in patients with low standards of hygiene, but is sometimes found in non-tropical countries, in patients with minor underlying lesions.201324382051
orbital myiasis caused by green bottle fly.an 80-year-old farmer, presented with large, maggot infested ulceration involving the medial part of the right upper lid. the left eye was phthisical. there was history of untreated traumatic laceration of the right upper lid. mechanical removal of maggots was done under turpentine coverage with regular antibiotic dressing. microbiological examination of maggots revealed the larvae to be of lucilia sericata (green bottle fly). the ulceration completely healed in two weeks following manual remova ...201324223066
multiple actions of lucilia sericata larvae in hard-to-heal wounds: larval secretions contain molecules that accelerate wound healing, reduce chronic inflammation and inhibit bacterial infection.in europe ≈15,000 patients receive larval therapy for wound treatment annually. over the past few years, clinical studies have demonstrated the success of larvae of lucilia sericata as debridement agents. this is based on a combination of physical and biochemical actions. laboratory investigations have advanced our understanding of the biochemical mechanisms underlying the beneficial effects of larval secretions, including removal of dead tissue, reduction of the bacterial burden, and promotion ...201324123092
proteolytic activity regarding sarconesiopsis magellanica (diptera: calliphoridae) larval excretions and secretions.sarconesiopsis magellanica (diptera: calliphoridae) is a medically important necrophagous fly which is used for establishing the post-mortem interval. diptera maggots release proteolytic enzymes contained in larval excretion and secretion (es) products playing a key role in digestion. special interest in proteolytic enzymes has also been aroused regarding understanding their role in wound healing since they degrade necrotic tissue during larval therapy. this study was thus aimed at identifying a ...201324076089
artificial neural network analysis of hydrocarbon profiles for the ageing of lucilia sericata for post mortem interval estimation.in analytical chemistry large datasets are collected using a variety of instruments for multiple tasks, where manual analysis can be time-consuming. ideally, it is desirable to automate this process while obtaining an acceptable level of accuracy, two aims that artificial neural networks (anns) can fulfil. anns possess the ability to classify novel data based on their knowledge of the domain to which they have been exposed. anns can also analyse non-linear data, tolerate noise within data and ar ...201324053861
growth and survival of bagged lucilia sericata maggots in wounds of patients undergoing maggot debridement therapy.maggot debridement therapy (mdt) is an established method of debridement of nonhealing wounds. despite intense clinical research about its efficacy and effects of substances produced by the larvae, growth and development of maggots in the wounds remain largely unexplored. in the present study, the bags with larvae (n = 52), which had been used to debride traumatic, ischemic, diabetic and venous ulcers, were collected and examined. survival, length, width and larval instar of the maggots within e ...201323983774
antennal sensilla of the green bottle fly, lucilia sericata (meigen) (diptera: calliphoridae).lucilia sericata (meigen) is a cosmopolitan synanthropic fly of forensic and medical importance, which can work as a mechanic vector of pathogens or cause myiasis of both human and sheep. as essential olfactory organs, antennae of adult l. sericata were examined with stereoscopic microscope, scanning electron microscope, and laser scanning confocal microscope. on antennal scape and pedicel, both microtirchiae and several bristles are detected, while another two structures, setiferous plaques and ...201323955594
insects colonising carcasses in open and forest habitats of central europe: search for indicators of corpse relocation.several traces may reveal the post-mortem relocation of a corpse. insects are particularly useful for that purpose. the use of insects for inferring the transfer of a corpse rests on a premise that particular species colonise corpses in different habitats. however, only some insects reveal a strong preference for a given type of habitat. in order to find those insects which colonise corpses exclusively in open habitats, as opposed to forest habitats, a pig carrion study was made in rural open an ...201323890643
degradation of mscramm target macromolecules in vlu slough by lucilia sericata chymotrypsin 1 (isp) persists in the presence of tissue gelatinase activity.venous leg ulcer slough is unpleasant to the patient and difficult to manage clinically. it harbours infection, also preventing wound management materials and dressings from supporting the underlying viable tissues. in other words, slough has significant nuisance value in the tissue viability clinic. in this study, we have sought to increase our knowledge of slough by building upon a previous but limited analysis of this necrotic tissue. in particular, slough has been probed using western blotti ...201523834475
increasing precision in development-based postmortem interval estimates: what's sex got to do with it?forensic entomologists typically use either succession models for postmortem interval (pmi) estimates or development-based models for minimum pmi (pmi(min) estimates. development-based age estimates are calculated with durations of immature stadia and can also include morphological data such as larval size. for developmental data, the first and second instar stages are typically brief with little variation in larval length. the third instar, a much longer stage by comparison, is prone to conside ...201323540132
a case of oral myiasis caused by lucilia sericata (diptera: calliphoridae) in korea.we report here a case of oral myiasis in the republic of korea. the patient was a 37-year-old man with a 30-year history of becker's muscular dystrophy. he was intubated due to dyspnea 8 days prior to admission to an intensive care unit (icu). a few hours after the icu admission, 43 fly larvae were found during suction of the oral cavity. all maggots were identified as the third instars of lucilia sericata (diptera: calliphoridae) by morphology. we discussed on the characteristics of myiasis acq ...201323467858
effect of ketamine on the development of lucilia sericata (meigen) (diptera: calliphoridae) and preliminary pathological observation of larvae.the estimation of postmortem interval (pmi) based on the growth patterns of necrophagous arthropods is the main mission of forensic entomology in practice. the larval development rates can be affected by various drugs or toxins, causing deviation in pmi estimate. ketamine is a widely used anesthetic and recreational drug in asia, which is rarely focused on in the previous entomotoxicological studies. the present work investigated the effect of ketamine on the development of lucilia sericata (mei ...201323434378
discontinuous foraging behavior of necrophagous lucilia sericata (meigen 1826) (diptera calliphoridae) larvae.larvae of the necrophagous blowfly lucilia sericata (diptera calliphoridae) live on vertebrate cadavers. although continuously feeding was previously assumed for this species, we hypothesized that larvae do not feed constantly. according to this hypothesis, their crop should not always be full, which should be reflected in crop surfaces. we dissected and measured the crops of larvae of the same age and bred in the same conditions. crop surfaces of 117 larvae just removed from the food ranged fro ...201323333403
a case of human oral myiasis by lucilia sericata in a hospitalized patient in extremadura, spain.myiasis is the term used to describe infestations, both obligatory and accidental, in vertebrate animals and humans by dipteral larvae. the oral cavity is rarely affected by this infestation and the circumstances which can lead to oral myiasis include persistent mouth opening together with poor hygiene, or facial traumatism. we present a case of oral myiasis by larvae of lucilia sericata, a species present in the iberian peninsula, in a hospitalized patient with surgical problems.201223304161
occurrence of nasal nosocomial myiasis by lucilia sericata (diptera: calliphoridae) in north of iran.we report a case of human nasal nosocomial myiasis in a 69-year-old rural man with numerous live maggots in nostrils. the patient was admitted in emergency ward due to dyspnea. after 72 hours the companion of patient discovered larva. the presence of the third instar larva indicated that the infestation was not more than three days. the collected instars were cleared, fixed and morphological studies then were carried out precisely and the larva was identified as lucilia sericata.201223133480
potential use of hydrocarbons for aging lucilia sericata blowfly larvae to establish the postmortem interval.previous studies on diptera have shown the potential for the use of cuticular hydrocarbons' analysis in the determination of larval age and hence the postmortem interval (pmi) for an associated cadaver. in this work, hydrocarbon compounds, extracted daily until pupation from the cuticle of the blowfly lucilia sericata (diptera: calliphoridae), have been analyzed using gas chromatography-mass spectrometry (gc-ms). the results show distinguishing features within the hydrocarbon profile over the pe ...201323130882
biocompatibility of antimicrobials to maggot debridement therapy: medical maggots lucilia sericata (diptera: calliphoridae) exhibit tolerance to clinical maximum doses of antimicrobials.the larvae of calliphorid flies are used to debride wounds of patients with severe tissue destruction, often concurrently with antimicrobials. the current study evaluates the effects of ceftazidime, tobramycin, amikacin, gentamicin, polymyxin b, doxycycline, paromomycin, amphotericin b, sodium stibogluconate, and miltefosine at 1, 10, and 100 x the maximum clinical concentration (mg/kg/d) in raw liver assays. effects on larvae were small and depended on dose and antimicrobial formulation, with h ...201223025196
wound debridement potential of glycosidases of the wound-healing maggot, lucilia sericata.the wound-healing maggot, lucilia sericata meigen (diptera: calliphoridae), degrades extracellular matrix components by releasing enzymes. the purpose of this study was to investigate the glycosylation profiles of wound slough/eschar from chronic venous leg ulcers and the complementary presence of glycosidase activities in first-instar excretions/secretions (es1) and to define their specificities. the predominant carbohydrate moieties present in wound slough/eschar were determined by probing one ...201222620282
the combined algorithm of venous ulcer therapy.this prospective study was undertaken to find a new combined algorithm to help patients suffering from long-term chronic non-healing venous ulcers.201222616586
the use of maggot debridement therapy in the treatment of chronic wounds in hospitalised and ambulatory patients.to summarise our experience of the use of medicinal maggots for the debridement of necrotic chronic wounds and to try and identify prognostic factors for debridement success and associated pain.201222584527
effect of different post-feeding intervals on the total time of development of the blowfly lucilia sericata (diptera: calliphoridae).by estimating the age of the immature stages of flies developing on a corpse, forensic entomologists are able to establish the minimum post-mortem interval. blowflies, which are the first and most important colonizers, usually leave the cadaver at the end of the last larval stage searching for a pupation site. this period of development is referred as the post-feeding or wandering stage. the characteristics of the ground where the corpse was placed might be of notable importance for the post-fee ...201222551693
nasal myiasis due to lucilia sericata (meigen, 1826) from iran: a case report.although not very common, human myiasis occurs more in developing tropical countries. a variety of dipterans cause nasal myiasis including the family caliphoridae. in this report a case of nasal myiasis due to lucilia sericata is reported for the first time, in a 74-year old patient from mazandaran heart centre, north of iran. the patient was originally admitted to the mazandaran heart centre due to cardiac arrest and cerebral ischemia with diminishing consciousness. because of the emergence of ...201222543618
[poorly healing periorbital wounds. therapeutic use of maggots].the treatment of poorly healing wounds, although not a typical problem in the periorbital area, has been enriched by the option of biosurgery, the therapeutic application of larvae of the blow fly (lucilia sericata).201222532045
a case of traumatic myiasis in a domestic rabbit (oryctolagus cuniculus) caused by lucilia sericata.lucilia sericata is one of the factors resulting in facultative traumatic myiasis in animals and humans. l. sericata threatens human health and leads to significant economic losses in animal industry by leading to serious parasitic infestations. a three month old female rabbit was presented to the clinics of the veterinary faculty of dicle university for the treatment of the wound located on the left carpal joint. the examination revealed that the wound was infested with larvae. the microscopic ...201222450925
plant terpenoids: acute toxicities and effects on flight motor activity and wing beat frequency in the blow fly phaenicia sericata.we evaluated the acute toxicities and the physiological effects of plant monoterpenoids (eugenol, pulegone, citronellal and alpha-terpineol) and neuroactive insecticides (malathion, dieldrin and rh3421) on flight muscle impulses (fmi) and wing beat signals (wbs) of the blow fly (phaenicia sericata). topically-applied eugenol, pulegone, citronellal, and alpha-terpineol produced neurotoxic symptoms, but were less toxic than malathion, dieldrin, or rh3421. topical application of eugenol, pulegone, ...201222420258
the composition of the cuticular and internal free fatty acids and alcohols from lucilia sericata males and females.gc, gc-ms, and hplc-llsd analyses were used to identify and quantify cuticular and internal lipids in males and females of the blow-fly (lucilia sericata). sixteen free fatty acids, seven alcohols and cholesterol were identified and quantitatively determined in the cuticular lipids of l. sericata. cuticular fatty acids ranged from c(6) to c(20) and included unsaturated entities such as 16:1n-9, 18:1n-9, 20:4n-3 and 20:5n-3. cuticular alcohols (only saturated and even-numbered) ranged from c(12) ...201222415221
disentangling the functional consequences of the connectivity between optic-flow processing neurons.typically, neurons in sensory areas are highly interconnected. coupling two neurons can synchronize their activity and affect a variety of single-cell properties, such as their stimulus tuning, firing rate or gain. all of these factors must be considered to understand how two neurons should be coupled to optimally process stimuli. we quantified the functional effect of an interaction between two optic-flow processing neurons (vi and h1) in the fly (lucilia sericata). using a generative model, we ...201222327473
the insect defensin lucifensin from lucilia sericata. 201222322867
a de novo transcriptome assembly of lucilia sericata (diptera: calliphoridae) with predicted alternative splices, single nucleotide polymorphisms and transcript expression estimates.the blow fly lucilia sericata (diptera: calliphoridae) (meigen) is a nonmodel organism with no reference genome that is associated with numerous areas of research spanning the ecological, evolutionary, medical, veterinary and forensic sciences. to facilitate scientific discovery in this species, the transcriptome was assembled from more than six billion bases of illumina and twenty-one million bases of 454 sequence derived from embryonic, larval, pupal, adult and larval salivary gland libraries. ...201222283785
cutaneous myiasis in a geriatric patient.cutaneous myiasis in humans involving fly species endemic in italy are uncommon and are not often present in the literature. in the present article we describe the case of cutaneous myiasis in a woman brought to the emergency department (ed) of the s. croce e carle general hospital in cuneo, northwest italy. the patient was in precarious hygienic condition, and was suffering from localized ulcerated foot ulcers with significant inflammation extending up to the knee. fly larvae, subsequently iden ...201022320020
human urogenital myiasis caused by lucilia sericata (diptera: calliphoridae) and wohlfahrtia magnifica (diptera: sarcophagidae) in markazi province of iran.we report a case of human urogenital myiasis in an 86-year-old rural man with a penil ulcer and numerous alive and motile larvae from urethra and glans penis. entomological studies on adult flies showed the larvae were lucilia sericata and wohlfahrtia magnifica. the clinical presentation and treatment strategies are discussed.201022808392
influences of prey density on fecundity in a mantis, paratenodera angustipennis (s.).to clarify the effects of prey density on fecundity in predatory insects, relationships between density of prey (adult flies of phaenicia sericata) and number of eggs, preoviposition periods, and egg size were examined, using adult female mantids, paratenodera angustipennis de saussure. the results are summarized as follows. 1) the relationship between prey density and number of flies captured by a mantis per day was described by a type ii functional response curve. 2) the relationship between p ...198328310208
an experimentally tested model of the cumulative mating frequency curve for lucilia sericata (mg.) (dipt., calliphoridae). 197122894873
sterility and the mated status test in tepa-treated male sheep blow-flies, lucilia sericata (mg.) (dipt., calliphoridae). 197122894872
mutant genes and the emigration behavior of phaenicia sericata (diptera, calliphoridae). 196928562967
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