| differential expression of two glucocorticoid receptors in seabass (teleost fish) head kidney after exogeneous cortisol inoculation. | stressful conditions include a prompt release of corticosteroid hormones which can mediate gene expression through glucocorticoid receptors (gr). since two seabass (dicentrarchus labrax) grs have been cloned and sequenced from peritoneal cavity cells (dlgr1) and liver (dlgr2), a comparative amino acid sequence analysis that included haplochromis burtoni hbgrs, was carried out and homologies disclosed. the dlgr1 and dlgr2 deduced aminoacid sequences showed 61% identity (i) and 70% similarity (s). ... | 2010 | 20460166 |
| analysis of single nucleotide polymorphisms in three chromosomes of european sea bass dicentrarchus labrax. | single nucleotide polymorphisms (snps) are believed to contain relevant information and have been therefore extensively used as genetic markers in population and conservation genetics, and molecular ecology studies. this study reports on the identification of potential snps in a diploid european sea bass dicentrarchus labrax genome by using reference sequences from three assembled chromosomes and mapping all wgs datasets onto them (3× sanger, 3× 454 and 20× solexa). a total of 20,779 snps were i ... | 2011 | 20452842 |
| mixture toxicity assessment of nickel and chlorpyrifos in the sea bass dicentrarchus labrax. | the present research work was designed to study dicentrarchus labrax biotransformation and detoxification responses to acute exposure to nickel (ni) and chlorpyrifos (chp). sexually immature sea bass were treated by intraperitoneal injection of nickel chloride (500 μg kg⁻¹), chlorpyrifos (10 mg kg⁻¹), and their binary mixture for 1, 3, and 7 days. ni and chp accumulation was quantified in liver after the exposure periods. the following biological responses were measured: (1) nadph cytochrome p45 ... | 2011 | 20422167 |
| response of european sea bass (dicentrarchus labrax) to graded levels of methionine (total sulfur amino acids) in soya protein-based semi-purified diets. | the dietary methionine (met) and total sulfur amino acid (tsaa) requirements of european sea bass (dicentrarchus labrax) (initial body weight 13.4 (sd 0.2) g) were estimated in a 12-week dose-response experiment. seven isonitrogenous (7.6 % dm) and isoenergetic (gross energy, 21.2 mj/kg dm) diets, based on soya protein and crystalline l-amino acids containing graded levels of l-met (1.6-16.2 g/kg) at a constant cysteine (4 g/kg) level and a fish meal-based diet, were fed each to triplicate group ... | 2010 | 20416128 |
| regulation of fads2 expression and activity in european sea bass (dicentrarchus labrax, l.) fed a vegetable diet. | supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. in this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of european sea bass (dicentrachus labrax) was investigated. two groups of european sea bass were fed with fish diet (fd) or vegetable diet ( ... | 2010 | 20363355 |
| autonomic regulation of the heart during digestion and aerobic swimming in the european sea bass (dicentrarchus labrax). | the autonomic regulation of the heart was studied in european sea bass (dicentrarchus labrax) during digestion and aerobic exercise by measuring cardiac output (q), heart rate (f(h)), stroke volume (v(s)) and oxygen consumption (mo(2)) before and after pharmacological blockade by intraperitoneal injections of atropine and propranolol. the significant rise in mo(2) (134+/-14 to 174+/-14 mg kg(-)(1)h(-)(1)) 6h after feeding (3% body mass) caused a significant tachycardia (47.7+/-10.9 to 72.6+/-7.2 ... | 2010 | 20362690 |
| swimming ability and ecological performance of cultured and wild european sea bass (dicentrarchus labrax) in coastal tidal ponds. | locomotor performance is commonly used to predict ecological performance of animals and is often considered a proxy for darwinian fitness. in fish, swimming performance is often measured in the laboratory, but its contribution to individual success in the field is rarely evaluated. we assessed maximal swimming velocity of wild and cultured juvenile dicentrarchus labrax (european sea bass) in a sprint performance chamber and found substantial variation among individuals within a cohort and differ ... | 2010 | 20345243 |
| cage exposure of european sea bass (dicentrarchus labrax) for in situ assessment of pollution-related genotoxicity. | genotoxic effects are often the earliest signs of pollution-related environmental disturbance. in this study, we used the comet assay and micronucleus test to assess dna damage in the erythrocytes of the european sea bass (dicentrarchus labrax) exposed to environmental pollution in situ. fish were collected from a fish farm in the trogir bay and their cages placed at an unpolluted reference site solta (necujam bay) and a polluted site vranjic (kastela bay) for four weeks. a group of fish which r ... | 2010 | 20338865 |
| physiological mechanisms underlying a trade-off between growth rate and tolerance of feed deprivation in the european sea bass (dicentrarchus labrax). | the specific growth rate (sgr) of a cohort of 2000 tagged juvenile european sea bass was measured in a common tank, during two sequential cycles comprising three-weeks feed deprivation followed by three-weeks ad libitum re-feeding. after correction for initial size at age as fork length, there was a direct correlation between negative sgr (rate of mass loss) during feed deprivation and positive sgr (rate of compensatory growth) during re-feeding (spearman rank correlation r=0.388, p=0.000002). f ... | 2010 | 20228351 |
| molecular cloning, tissue distribution, and daily rhythms of expression of per1 gene in european sea bass (dicentrarchus labrax). | circadian rhythms are controlled by interlocked autoregulatory feedback loops consisting of interactions of a group of circadian clock genes and their proteins. the period family is a group of genes that are essential components of the molecular clock. in the present study, we cloned a period gene (per1) of the european sea bass, a marine teleost of chronobiological interest. the cloned sequence encoded a protein consisting of 1436 amino acids that homology and phylogenic analyses showed to be r ... | 2010 | 20205555 |
| assessment of contaminants and biomarkers of exposure in wild and farmed seabass. | this study aimed to investigate the relationships between levels of organochlorine compounds (pcbs and ddts), polycyclic aromatic hydrocarbons (pahs), metals and biomarkers of exposure (erod and gst) and oxidative stress biomarkers, in wild and cultured european seabass (dicentrarchus labrax). additionally, the fatty acid profile was determined in muscle to assess differences between cultivated and wild specimens of marketable size. although results pointed to relatively low concentration of con ... | 2010 | 20167373 |
| leptin-like immunoreactivity in the muscle of juvenile sea bass (dicentrarchus labrax). | the mammalian hormone, leptin, is mainly synthesized in adipose tissue along with other tissues. leptin plays a role in numerous processes such as in the control of food intake, homeostasis, immune function and reproduction. in this study, we detected and localized leptin immunoreactivity to the muscle of early juvenile sea bass (dicentrarchus labrax) by western blot analysis and immunohistochemistry. a leptin immunopositive band with a molecular weight of approximately 16 kda, corresponding to ... | 2010 | 20146346 |
| the european sea bass dicentrarchus labrax genome puzzle: comparative bac-mapping and low coverage shotgun sequencing. | food supply from the ocean is constrained by the shortage of domesticated and selected fish. development of genomic models of economically important fishes should assist with the removal of this bottleneck. european sea bass dicentrarchus labrax l. (moronidae, perciformes, teleostei) is one of the most important fishes in european marine aquaculture; growing genomic resources put it on its way to serve as an economic model. | 2010 | 20105308 |
| poly-beta-hydroxybutyrate (phb) increases growth performance and intestinal bacterial range-weighted richness in juvenile european sea bass, dicentrarchus labrax. | the bacterial storage polymer poly-beta-hydroxybutyrate (phb) has the potential to be used as an alternative anti-infective strategy for aquaculture rearing. in this research, the effects of (partially) replacing the feed of european sea bass juveniles with phb were investigated. during a 6-week trial period, the phb showed the ability to act as an energy source for the fish. this indicated that phb was degraded and used during gastrointestinal passage. the gut ph decreased from 7.7 to 7.2 sugge ... | 2010 | 20094715 |
| embryonic occurrence of ionocytes in the sea bass dicentrarchus labrax. | because of the permeability of the chorion, sea bass embryos are exposed to seawater before hatching and hence require precocious osmoregulatory processes. several studies of other species have demonstrated the existence of ion-transporting cells located on the yolk sac membrane of embryos. in these cells, called ionocytes, ion movements are controlled by a pool of transmembrane proteins. among them, the na(+)/k(+)-atpase, an abundant driving enzyme, has been used to reveal the presence or absen ... | 2010 | 20066548 |
| immunolocalization of corticotropin-releasing factor (crf) and corticotropin-releasing factor receptor 2 (crf-r2) in the developing gut of the sea bass (dicentrarchus labrax l.). | our previous data indicated an important role for adrenocorticotropic (acth)-like molecules co-operating with macrophages to control the modifications in body homeostasis during the first period of the life of sea bass (up to 30 days post-hatching) before the lymphoid cells have reached complete maturation. the aim of the study was to determine the immunolocalization of corticotropin-releasing factor (crf), which is a very important mediator of stress-related responses. our data showed that immu ... | 2011 | 20045176 |
| qtl for body weight, morphometric traits and stress response in european sea bass dicentrarchus labrax. | natural mating and mass spawning in the european sea bass (dicentrarchus labrax l., moronidae, teleostei) complicate genetic studies and the implementation of selective breeding schemes. we utilized a two-step experimental design for detecting qtl in mass-spawning species: 2122 offspring from natural mating between 57 parents (22 males, 34 females and one missing) phenotyped for body weight, eight morphometric traits and cortisol levels, had been previously assigned to parents based on genotypes ... | 2010 | 20028379 |
| an investigation of metabolic prioritization in the european sea bass, dicentrarchus labrax. | we investigated the ability of european sea bass (dicentrarchus labrax) to respond simultaneously to the metabolic demands of specific dynamic action (sda) and aerobic exercise and how this was influenced by moderate hypoxia (50% air saturation). at 3 h after feeding in normoxia at 20 degrees c, sda raised the instantaneous oxygen uptake (mo(2)) of sea bass by 47% +/- 18% (mean +/- sem, n = 7) above their standard metabolic rate (smr) when fasted. this metabolic load was sustained throughout an ... | 2016 | 19951229 |
| anchoring novel molecular biomarker responses to traditional responses in fish exposed to environmental contamination. | the responses of dicentrarchus labrax and liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. dna damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome p450 1a1 gene expression were not significantly altered. the liver histopathological analysis also revealed significant lesions in fish from contaminated sites. alterations in ras and xpf genes we ... | 2010 | 19944504 |
| detection of antimicrobial peptides related to piscidin 4 in important aquacultured fish. | epithelial surfaces of fish, such as the gut, skin and gills, comprise a large surface area for possible pathogen invasion. antimicrobial peptides (amps), innate immunity components, play a significant role in protecting fish. piscidins are a family of amps. in this study, we detected the presence of the recently discovered piscidin 4 via bug blot, western blot, elisa and/or immunohistochemistry in striped bass (morone saxatilis), white bass (m. chrysops), european seabass (dicentrarchus labrax) ... | 2010 | 19913049 |
| evolution of a multifunctional gene: the warm temperature acclimation protein wap65 in the european seabass dicentrarchus labrax. | the warm temperature acclimation protein wap65 has been shown to be involved in temperature acclimation, in immune response as well as in development. in teleosts, two types of wap65 proteins, wap65-1 and wap65-2 are found, both acting as a multifunctional agent in several biological processes. in the present study we identified both transcripts wap65-1 and wap65-2 for the european seabass (dicentrarchus labrax), examined their evolutionary rate and performed selection tests. the two paralogues ... | 2010 | 19853054 |
| identification and characterisation of a novel immune-type receptor (nitr) gene cluster in the european sea bass, dicentrarchus labrax, reveals recurrent gene expansion and diversification by positive selection. | in the last decade, a new gene family encoding non-rearranging receptors, called novel immune-type receptors (nitrs), has been discovered in teleost fish. nitrs belong to the immunoglobulin superfamily and represent an extraordinarily divergent and rapidly evolving gene complex. genomic analysis of a region spanning 270 kb led to the discovery of a nitr gene cluster in the european sea bass (dicentrarchus labrax). in total, 27 nitr genes and three putative pseudogenes, organised in a tandemly ar ... | 2009 | 19851764 |
| nodavirus infection of sea bass (dicentrarchus labrax) induces up-regulation of galectin-1 expression with potential anti-inflammatory activity. | sea bass nervous necrosis virus is the causative agent of viral nervous necrosis, a disease responsible of high economic losses in larval and juvenile stages of cultured sea bass (dicentrarchus labrax). to identify genes potentially involved in antiviral immune defense, gene expression profiles in response to nodavirus infection were investigated in sea bass head kidney using the suppression subtractive hybridization (ssh) technique. a total of 8.7% of the expressed sequence tags found in the ss ... | 2009 | 19846879 |
| bioaccumulation markers and biochemical responses in european sea bass (dicentrarchus labrax) raised under different environmental conditions. | site- and season-specific biochemical responses and bioaccumulations of organochlorine pesticides (ocps) and polychlorinated biphenyls (pcbs) were analysed in tissues from sea bass raised under four different environmental conditions and sampled in april and july. samples were analysed for condition factor (cf), liver somatic index (lsi), glutathione s-transferase (gst) activity, and glycogen and lactate contents. results showed the presence of pcbs and ddts, with site- and season-specific varia ... | 2010 | 19819549 |
| growth heterogeneity in rearing sea bass ( dicentrarchus labrax): test of hypothesis with an iterative energetic model. | this study aimed at modeling the relative importance of food intake on growth heterogeneity among cultured sea bass (dicentrarchus labrax). first, we designed an individual growth model comprising five compartments (energy intake, losses, net energy, recovered energy and maintenance). this model was calibrated with a first experiment carried out in eight tanks; a total of 130 juveniles (11 g) per tank were fed by a self-feeder (84 days, 20°c, 16l : 8d, 30 g nacl/l). a second experiment was perfo ... | 2009 | 22444906 |
| evaluation of the shelf life of vacuum- and modified atmosphere packaged dicentrarchus labrax fillets. | | 2009 | 19578950 |
| effects of feeding and hypoxia on cardiac performance and gastrointestinal blood flow during critical speed swimming in the sea bass dicentrarchus labrax. | previous studies have shown that if european sea bass are exercised after feeding, they can achieve a significantly higher maximum metabolic rate (mmr) than when fasted. they can meet combined metabolic demands of digestion (specific dynamic action, sda) and maximal aerobic exercise, with no decline in swimming performance. if, however, exposed to mild hypoxia (50% saturation), bass no longer achieve higher mmr after feeding but they swim as well fed as fasted, due to an apparent ability to defe ... | 2009 | 19559805 |
| effect of conjugated linoleic acid on dietary lipids utilization, liver morphology and selected immune parameters in sea bass juveniles (dicentrarchus labrax). | increased energy content in fish feeds has led to an enhanced fat deposition, particularly in european sea bass, concerning fish farmers. inclusion of conjugated linoleic acid (cla) could reduce fat deposition as in other vertebrates. to determine if dietary cla affects fat deposition, lipid metabolism, lipid composition and morphology of different tissues, growth and selected immune parameters, european sea bass juveniles were fed 4 graded levels of cla (0, 0.5, 1 and 2%). growth and feed conve ... | 2009 | 19539046 |
| biological effects of diethylene glycol (deg) and produced waters (pws) released from offshore activities: a multi-biomarker approach with the sea bass dicentrarchus labrax. | diethylene glycol (deg) is largely used during oil and gas exploitation by offshore platforms. the aim of this work was to investigate if this compound induces direct molecular/cellular effects in marine organisms, or indirectly modulate those of produced waters (pws). sea bass (dicentrarchus labrax) were exposed to deg dosed alone or in combination with pws from an adriatic platform. a wide array of analysed biomarkers included cytochrome p450-dependent enzymatic activity, bile metabolites, glu ... | 2009 | 19515471 |
| nuclear abnormalities of marine fish erythrocytes. | the aim of this study was to monitor erythrocyte nuclear abnormalities (na) including micronuclei (mn) in cultured and wild sea bass dicentrarchus labrax and wild mullet mugil spp. seasonal sampling was performed at seven locations along the eastern coast of the adriatic sea. the frequency of na and mn was positively correlated to temperature (na: p < 0.05, r = 0.11; mn p < 0.05, r = 0.10), and there was also a positive correlation between na and mn frequency (p < 0.001, r = 0.43). the lowest na ... | 2009 | 20735550 |
| the compensatory growth in juveniles of sea bass: gastric distributive pattern of molecules regulating metabolism. | the aim of this work was to investigate the distribution of regulative molecules in the stomach of juvenile dicentrarchus labrax during compensatory growth, using immunohistochemical methods. antisera against galanin, neuropeptide y, ghrelin, leptin, and serotonin were used on fasted and refed d. labrax. the results show a characteristic distributive pattern for the sought molecules in fish refed after 35 days of fasting, with a high increased presence of both ghrelin and leptin. | 2009 | 19456368 |
| lymphocyte differentiation in sea bass thymus: cd4 and cd8-alpha gene expression studies. | different developmental stages (from eggs to 1-year-old juveniles) of the teleost fish dicentrarchus labrax (l.) were assayed for cd4 gene expression. rt-pcr revealed the appearance of cd4 transcripts in post-larvae from 51 days post-hatching (dph). this finding overlaps the first detection of cd8-alpha mrna. real-time pcr with specific primers quantified cd4, cd8-alpha and tcr-beta transcripts in larvae and post-larvae (25, 51, 75 and 92 dph) and 1-year-old thymus. at 92 dph, tcr-beta and cd8-a ... | 2009 | 19422917 |
| seasonal changes in gonadal expression of gonadotropin receptors, steroidogenic acute regulatory protein and steroidogenic enzymes in the european sea bass. | the endocrine regulation of gametogenesis, and particularly the roles of gonadotropins, is still poorly understood in teleost fish. this study aimed to investigate transcript levels of both gonadotropin receptors (fshr and lhr) during an entire reproductive cycle in male and female sea bass (dicentrarchus labrax). to have a more comprehensive understanding of how different key factors interact to control sea bass gonadal function, changes in the transcript abundance of two important steroidogeni ... | 2009 | 19345689 |
| thermally induced phenotypic plasticity of swimming performance in european sea bass dicentrarchus labrax juveniles. | the vulnerability of embryonic and larval stages of european sea bass dicentrarchus labrax to environmental temperature and the longer-term consequences for the early juveniles was demonstrated. this phenotypic plasticity was highlighted by subjecting d. labrax at 15.2 +/- 0.3 or 20.0 +/- 0.4 degrees c (mean +/-s.d.) up to metamorphosis and then at the same temperature (18.5 +/- 0.7 degrees c). after 4-6 weeks at the same temperature, the measurement of critical swimming speed at four exercise t ... | 2009 | 20735633 |
| influence of salinity on the localization and expression of the cftr chloride channel in the ionocytes of juvenile dicentrarchus labrax exposed to seawater and freshwater. | the european sea-bass, dicentrarchus labrax is a euryhaline teleost whose high osmoregulatory abilities allow sea-lagoon migrations. in order to investigate the mechanism underlying the acclimation of juvenile fish to salinity, cftr was studied in long-term (6 months) freshwater (fw)- and seawater (sw)-exposed fish, and in short-term (from day 0 to day 30) fw-exposed fish. cellular and molecular approaches were combined to determine the functions of cftr in the gills, posterior intestine and kid ... | 2009 | 19328865 |
| influence of salinity on the localization and expression of the cftr chloride channel in the ionocytes of dicentrarchus labrax during ontogeny. | the expression and localization of the cystic fibrosis transmembrane conductance regulator (cftr) were determined in four osmoregulatory tissues during the ontogeny of the sea-bass dicentrarchus labrax acclimated to fresh water and sea water. at hatch in sea water, immunolocalization showed an apical cftr in the digestive tract and integumental ionocytes. during the ontogeny, although cftr was consistently detected in the digestive tract, it shifted from the integument to the gills. in fresh wat ... | 2009 | 19245499 |
| acute toxicity of ochratoxin-a in marine water-reared sea bass (dicentrarchus labrax l.). | the toxic effects of ochratoxin-a (ota) on sea bass, dicentrarchus labrax l., have not been previously documented. a flow-through bioassay test system was conducted in two series and a total 180 of adult marine-reared sea bass was used to estimate the acute oral 96 h median lethal concentration (lc(50)) value and behavioral changes of ota. the data obtained were statistically evaluated using finney's probit analysis method developed by epa. the 96 h lc(50) value for adult d. labrax was found to ... | 2009 | 19230951 |
| effect of temperature on muscle lactate metabolic recovery in sea bass (dicentrarchus labrax, l.) juveniles exposed to exhaustive exercise. | muscle lactate metabolic recovery in sea bass juveniles was studied in respect to the water temperature. the fish used in this study were cultured with the semi-extensive mesocosm method, and the water temperatures were 15 and 20 degrees c. fish were led to exhaustion by exercising in a swimming channel, and the muscle lactate levels were measured seven times before and after exercise. the results showed that muscle lactate concentration rises during exercise until it reaches a maximum (exhausti ... | 2010 | 19224385 |
| effects of exposure to overcrowding on rodlet cells of the teleost fish dicentrarchus labrax (l.). | farmed fish are usually exposed to routine procedures which have strong effects on stress responses. rodlet cells may represent an useful biomarker for studies on the presence of stressors in aquaculture. this work focused on the localization of rodlet cells by light and electron microscopy in gills of sea bass (dicentrarchus labrax) subjected to different conditions of overcrowding. in general, a significant increase in number of rodlet cells has been observed in all animals subjected to overcr ... | 2009 | 19205917 |
| in vivo regulation of glut2 mrna in sea bass (dicentrarchus labrax) in response to acute and chronic hypoxia. | the expression and regulation of sodium-independent glucose transporter (glut)-2, in relation to hypoxia has not yet been explored in fish or other vertebrates. in this study, the complete open-reading frame for sea bass glut2 was isolated and deposited in the genbank. the predicted 12 transmembrane domains of the protein (508 amino acids) are presented. a phylogenetic tree was constructed on glut2 sequences of sea bass and those of other teleost, amphibian, avian, and mammalian species. we also ... | 2009 | 19162213 |
| spermiogenic nuclear protein transitions and chromatin condensation. proposal for an ancestral model of nuclear spermiogenesis. | we have chosen three species (sparus aurata, dicentrarchus labrax, and monodonta turbinata) that represent different transition patterns in the composition and structure of spermiogenic nuclei. the transition patterns of these species are representative of spermiogenesis in a large number of animal species. we analyze: (a) nuclear protein exchange; (b) chromatin condensation pattern; and (c) histone acetylation during spermiogenic development. in the simplest spermiogenesis histones and nucleoso ... | 2009 | 19132734 |
| effects of two oils and 16 pure polycyclic aromatic hydrocarbons on plasmatic immune parameters in the european sea bass, dicentrarchus labrax (linné). | the in vitro effects of polycyclic aromatic hydrocarbons (pahs) on two plasmatic immune parameters, lysozyme concentration and haemolytic alternative complement activity, of the european sea bass, dicentrarchus labrax, were tested using field (10(-7) and 10(-9) mg ml(-1)) and high concentrations (10(-3) and 10(-5) mg ml(-1)) observed during oil spills. peripheral blood from 105 fish was collected, centrifuged at 1200 g, for 10 min, at 4 degrees c and three plasma pools, each of 35 fish, were con ... | 2009 | 19111921 |
| effects of water salinity on melatonin levels in plasma and peripheral tissues and on melatonin binding sites in european sea bass (dicentrarchus labrax). | sea bass is an euryhaline fish that lives in a wide range of salinities and migrates seasonally from lagoons to the open sea. however, to date, the influence of water salinity on sea bass melatonin levels has not been reported. here, we evaluated the differences in plasma and tissue melatonin contents and melatonin binding sites in sea bass under four different salinity levels: seawater (36 per thousand), isotonic water (15 per thousand), brackish water (4 per thousand) and freshwater (0 per tho ... | 2009 | 19110064 |
| different sox17 transcripts during sex differentiation in sea bass, dicentrarchus labrax. | sox genes participate in several developmental processes, including sex determination and differentiation. in this study, the genomic structure of sox17 was characterized in the sea bass (sb). two transcripts, one producing a normal protein (sb sox17) and another producing a truncated protein (sb t-sox17) were detected. a third, novel transcript, originated by intron retention (sb i-sox17) was also observed. sb sox17 was widely distributed, whereas sb i-sox17 was predominantly found in skin and ... | 2009 | 19071190 |
| masculinization of the european sea bass (dicentrarchus labrax) by treatment with an androgen or aromatase inhibitor involves different gene expression and has distinct lasting effects on maturation. | the objective of this study was to contribute to our understanding of the role of sex steroids in fish sex differentiation and male maturation. sexually undifferentiated sea bass were administered 17alpha-methyldihydrotestosterone (mdht), estradiol-17beta (e(2)), fadrozole (fz), cyproterone acetate (cpa) or tamoxifen (tx). mdht produced 100% males whereas e(2) and tx resulted in 100% females. fz produced 95% males while cpa did not alter sex ratios. e(2) treatment did not affect gonadal aromatas ... | 2009 | 18983844 |
| molecular characterisation and structural analysis of an interferon homologue in sea bass (dicentrarchus labrax l.). | the interferons (ifns) are a large family of soluble cytokines involved in the immune response against viral pathogens. three families of ifns have been identified in mammals (type i, type ii and type iii) and, recently, homologues of type i and type ii genes have been found in various teleost fish species. in this paper we report the cloning of a cdna encoding an type i ifn molecule from sea bass (dicentrarchus labrax l.), its expression analysis and gene structure and, finally, its 3d structur ... | 2009 | 18952288 |
| ontogenic effects of early feeding of sea bass (dicentrarchus labrax) larvae with a range of dietary n-3 highly unsaturated fatty acid levels on the functioning of polyunsaturated fatty acid desaturation pathways. | four replicated groups of sea bass (dicentrarchus labrax) larvae were fed diets containing an extra-high level of highly unsaturated fatty acids (hufa) (xh; 3.7 % epa+dha), a high level of hufa (hh; 1.7 %), a low level of hufa (lh; 0.7 %) or an extra-low level of hufa (xlh; 0.5 %) from day 6 to day 45 (experiment 1; xh1, hh1, lh1, xlh1). after a subsequent 1-month period feeding a commercial diet (2.7 % epa+dha), the capacity of the four initial groups to adapt to an n-3 hufa-restricted diet (0. ... | 2009 | 18838020 |
| a combined aflp and microsatellite linkage map and pilot comparative genomic analysis of european sea bass dicentrarchus labrax l. | european sea bass (dicentrarchus labrax l., moronidae, teleostei) sustains a regional fishery and is commonly farmed in the mediterranean basin, but has not undergone much long-term genetic improvement. an updated genetic linkage map of the european sea bass was constructed using 190 microsatellites, 176 amplified fragment length polymorphisms and two single nucleotide polymorphisms. from the 45 new microsatellite markers (including 31 type i markers) reported in this study, 28 were mapped. a to ... | 2008 | 18828863 |
| gastrointestinal blood flow and postprandial metabolism in swimming sea bass dicentrarchus labrax. | in trout and salmon, the metabolic costs of exercise and feeding are additive, which would suggest that gastrointestinal blood flow during exercise is maintained to preserve digestive and absorptive processes related to the specific dynamic action (sda) of food. however, in most published studies, gastrointestinal blood flow drops during swimming, hypoxia, and general stress. to test whether gastrointestinal blood flow is spared during exercise after feeding, sea bass were instrumented with flow ... | 2008 | 18752418 |
| sex steroid-induced inhibition of food intake in sea bass (dicentrarchus labrax). | this study was conducted to test the sensitivity to gonadal steroids of the systems regulating food intake in sea bass. animals were treated with silastic implants containing 17-beta-estradiol or testosterone. self-feeding was recorded for 31 days using computerized demand feeders and unfed-pellet recovery systems. both steroids strongly decreased self-feeding levels, feed efficiency and specific growth rates. the linear growth of fish treated with testosterone was higher than in 17-beta-estradi ... | 2009 | 18709378 |
| effects of persistent organic pollutants on the thyroid function of the european sea bass (dicentrarchus labrax) from the aegean sea, is it an endocrine disruption? | we evaluated the alterations of organochlorinated compounds such as polychlorobiphenyls (pcb), dichloro-diphenyl-dichloroethylene (dde) and dichloro-diphenyl-trichloroethane (ddt) on the thyroid in wild and cultured sea bass (dicentrarchus labrax) at environmental concentrations. these compounds influence the endocrine system of many fish species and are qualified as endocrine disruptors. the thyroid seems to be a target organ. two alteration endpoints: the thyroid histology and the muscular thy ... | 2008 | 18692207 |
| stage-specific gene expression during fish spermatogenesis as determined by laser-capture microdissection and quantitative-pcr in sea bass (dicentrarchus labrax) gonads. | the role of genes implicated in the regulation of spermatogenesis and their patterns of expression is still poorly understood. in this study, we took advantage of the cystic arrangement of the teleost testis to set up a laser capture microdissection procedure to isolate cells from cysts containing spermatogonia, spermatocytes, spermatids, or spermatozoa. we then used quantitative pcr to determine the stage-specific expression patterns of the germ cell marker vasa; gonadal aromatase (cyp19a); est ... | 2008 | 18614701 |
| sex-related changes in estrogen receptors and aromatase gene expression and enzymatic activity during early development and sex differentiation in the european sea bass (dicentrarchus labrax). | the present study addresses the role of aromatase and estrogen receptors in sex differentiation and development. with this purpose, a sea bass female- and a male-dominant group were obtained by successive size gradings since in this species females are already larger than males at the time of sex differentiation. changes in cyp19a and cyp19b gene expression and enzymatic activity were monitored by a validated real-time pcr and a tritiated water assay, respectively, during early development and s ... | 2008 | 18573255 |
| purification and characterization of follicle-stimulating hormone from pituitary glands of sea bass (dicentrarchus labrax). | follicle-stimulating hormone (fsh) was purified from pituitaries of sea bass (dicentrarchus labrax), and its biochemical and biological properties were studied. sea bass fsh (sbsfsh) was purified by ethanol extraction-precipitation (40-85%), followed by anion-exchange chromatography on a lkb ultropac tsk-deae column using a linear gradient of ammonium bicarbonate (50-1000 mm) and reverse phase chromatography on a resource 15rpc column with a linear gradient of acetonitrile (0-50%), using a fplc ... | 2008 | 18558403 |
| cloning and retinal expression of melatonin receptors in the european sea bass, dicentrarchus labrax. | melatonin contributes to synchronizing behaviors and physiological functions to daily and seasonal rhythm in fish. however, no coherent vision emerges because the effects vary with the species, sex, age, moment of the year or sexual cycle. and, scarce information is available concerning the melatonin receptors, which is crucial to our understanding of the role melatonin plays. we report here the full length cloning of three different melatonin receptor subtypes in the sea bass dicentrarchus labr ... | 2008 | 18555069 |
| lipoprotein lipase (lpl) is highly expressed and active in the ovary of european sea bass (dicentrarchus labrax l.), during gonadal development. | the oocytes of many fish species accumulate high amounts of neutral lipids as a caloric reserve for embryonic and larval development. we propose that lipoprotein lipase (lpl, ec 3.1.1.34) plays an important role in supplying the oocytes with fatty acids and we have cloned its cdna from the ovary of sea bass, and determined the patterns of lpl activity and lpl mrna expression in the ovary. the cdna obtained was 3051 bp long with an open reading frame encoding 518 amino acids. the amino acid seque ... | 2008 | 18511315 |
| environmental hypoxia as a metabolic constraint on fish: the case of atlantic cod, gadus morhua. | hypoxia is known to provoke a wide range of effects on aquatic animals. here we use laboratory and field data on atlantic cod, gadus morhua, to illustrate that many of these responses can be explained within the metabolic scope (ms) framework, i.e. taking into account the directive and limiting effects of dissolved oxygen (do) on the ability of animals to acquire energy for growth and activity. a ms model for cod shows that scope for activity (swimming, feeding, etc.) is proportional to do and b ... | 2008 | 18508091 |
| endogenous modification of macronutrient selection pattern in sea bass (dicentrarchus labrax, l.). | the use of a single diet with a well defined composition to feed fish throughout their life cycle is an oversimplification that probably does not respond to their metabolic requirements. for example, the seasonal reproduction that characterizes most fish species demands changes in nutritional requirements. bearing this in mind, the macronutrient selection pattern was studied from january to august in twelve individually housed sea bass exposed to a constant photoperiod (12l:12d h) and temperatur ... | 2008 | 18505702 |
| adaptation of the sea-bass (dicentrarchus labrax) to fresh water: role of aquaporins and na+/k+-atpases. | sea-bass (dicentrarchus labrax) grow under different salinity regimes, from the open sea to lagoons and even rivers, but some mortality has been recorded in juvenile stages when exposed to low salinity water. changes in water permeability of different osmoregulatory tissues could be the cause of reduction in blood osmotic pressure and death in some fish in fresh water (fw). in order to explore this condition, we have studied the changes of aquaporins (aqp1 and aqp3), alpha1 and alpha4 na(+)/k(+) ... | 2008 | 18485772 |
| glucocorticoid receptor (dlgr1) is expressed in pre-larval and larval stages of the teleost fish dicentrarchus labrax. | glucocorticoid hormone receptors (gr), members of the nuclear hormone receptor superfamily, are ligand-dependent transcription factors expressed in various tissues by binding to specific dna sequences. since glucocorticoids have a role in maintaining the homeostatic status in fish, we previously cloned and sequenced a gr (dlgr1) of adult dicentrarchus labrax; we also showed mrna expression (in situ hybridization) and tissue immunohistochemical localization of dlgr1 in several organs. this work h ... | 2008 | 18463897 |
| distribution of estrogen receptor 2 mrnas (esr2a and esr2b) in the brain and pituitary of the sea bass (dicentrarchus labrax). | three different estrogen receptors (ers) have been characterized in teleost fish, i.e. esr1, esr2a and esr2b. in this study we carried out in situ hybridizations in the brain and pituitary of the sea bass (dicentrarchus labrax) to study the putative involvement of esr2 subtypes in the control of gonadotropin secretion in fish. our studies demonstrated that both receptors are expressed within the main hypophysiotrophic areas of the sea bass brain thus providing neuroanatomical basis for the invol ... | 2008 | 18407250 |
| a cytoarchitectonic study of the brain of a perciform species, the sea bass (dicentrarchus labrax): the midbrain and hindbrain. | this study is the third part of a comprehensive series of publications on the cytoarchitectonic organization of the brain of the european sea bass, dicentrarchus labrax. this study provides an atlas of the brain stem based on nissl-stained transverse sections as well as a description of cell masses and a discussion on comparative aspects of brain stem nuclei, including methodological studies in other species. by external examination, the sea bass exhibits a prominent optic tectum and corpus cere ... | 2008 | 18406450 |
| a cd4 homologue in sea bass (dicentrarchus labrax): molecular characterization and structural analysis. | cd4 is a transmembrane glycoprotein fundamental for cell-mediated immunity. its action as a t cell co-receptor increases the avidity of association between a t cell and an antigen-presenting cell by interacting with portions of the complex between mhc class ii and tr molecules. in this paper we report the cdna cloning, expression and structural analysis of a cd4 homologue from sea bass (dicentrarchus labrax). the sea bass cd4 cdna consists of 2071 bp that translates in one reading frame to give ... | 2008 | 18403019 |
| linking the genomes of nonmodel teleosts through comparative genomics. | recently the genomes of two more teleost species have been released: the medaka (oryzias latipes), and the three-spined stickleback (gasterosteus aculateus). the rapid developments in genomics of fish species paved the way to new and valuable research in comparative genetics and genomics. with the accumulation of information in model species, the genetic and genomic characterization of nonmodel, but economically important species, is now feasible. furthermore, comparison of low coverage gene map ... | 2008 | 18297360 |
| cellular localization of insulin-like growth factor-ii protein in the sea bass (dicentrarchus labrax) from hatching to adult. | the cellular localization of igf-ii protein was investigated during larval and postlarval developmental stages of sea bass (dicentrarchus labrax) by immunohistochemistry using antisera raised against sparus aurata igf-ii. at hatching, igf-ii immunoreactivity was already present in the skin, developing intestine and skeletal muscle. during larval life igf-ii protein was also observed in heart musculature, in kidney and gill epithelia as well as in liver. in fry skeletal muscle a moderate igf-ii i ... | 2008 | 18283636 |
| gene expression patterns during the larval development of european sea bass (dicentrarchus labrax) by microarray analysis. | during the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. to provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression o ... | 2008 | 18246396 |
| role of cholecystokinin and its antagonist proglumide on macronutrient selection in european sea bass dicentrarchus labrax, l. | teleost fish are able to adjust their energy intake when fed on pure macronutrient sources, although the exact mechanisms regulating macronutrient selection remain unknown. since cholecystokinin (cck) has been reported to modify macronutrient selection patterns in mammals, we explored the effect of cck administered orally to european sea bass on the selection of separately encapsulated macronutrients. cck doses of 0.05, 0.15 and 0.25 mg/kg bw administered in gelatine capsules for 5 consecutive d ... | 2008 | 18207473 |
| expression of a glucocorticoid receptor (dlgr1) in several tissues of the teleost fish dicentrarchus labrax. | since glucocorticoids have a role in maintaining the homeostatic status in fish, in the present paper mrna expression (in situ hybridization) and tissue immunohistochemical localization of a glucocorticoid receptor (dlgr1) in several dicentrarchus labrax organs are reported. riboprobe and specific antibodies were prepared by using the dlgr1 that has been previously cloned and sequenced from peritoneal cavity leukocytes. both mrna and receptor were identified in head kidney, spleen, gills, intest ... | 2008 | 18155116 |
| real-time polymerase chain reaction, in situ hybridization and immunohistochemical localization of insulin-like growth factor-i and myostatin during development of dicentrarchus labrax (pisces: osteichthyes). | the distribution of insulin-like growth factor-i (igf-i) and myostatin (mstn) was investigated in sea bass (dicentrarchus labrax) by real-time polymerase chain reaction (pcr), in situ hybridization (ish) and immunohistochemistry. real-time pcr indicated that igf-i mrna increased from the second day post-hatching and that this trend became significant from day 4. ish confirmed a strong igf-i mrna expression from the first week post-hatching, with the most abundant expression being detected in the ... | 2008 | 18071755 |
| dietary vitamin mix levels influence the ossification process in european sea bass (dicentrarchus labrax) larvae. | the influence of dietary vitamins on growth, survival, and morphogenesis was evaluated until day 38 of posthatching life in european sea bass larvae (dicentrarchus labrax). a standard vitamin mix (vm), at double the concentration of the u.s. national research council's recommendations, was incorporated into larval feeds at 0.5%, 1.5%, 2.5%, 4.0%, and 8.0% to give treatments vm 0.5, vm 1.5, vm 2.5, vm 4.0, and vm 8.0, respectively. the group fed the vm 0.5 diet all died before day 30. at day 38, ... | 2008 | 18032465 |
| the importance of quantifying inherent variability when interpreting stable isotope field data. | stable isotope data are often used to assess diet, trophic level, trophic niche width and the extent of omnivory. notwithstanding ongoing discussions about the value of these approaches, variations in isotopic signatures among individuals depend on inherent variability as well as differences in feeding habitats. remarkably, the relative contributions of diet variation and inherent variability to differences in delta(15)n and delta(13)c among individuals have not been quantified for the same spec ... | 2008 | 17999089 |
| effect of high pressure on the calpain-calpastatin system in fish muscle. | calpains (calcium-activated neutral proteases) of sea bass (dicentrarchus labrax l.) muscle may participate in the degradation of muscle tissue during postmortem storage. these enzymes are regulated by calpastatin, their endogenous specific inhibitor. the objective of this study was to evaluate the changes encountered by the calpain system during the postmortem storage of fish muscle after high-pressure treatment. from 100 mpa, high-pressure treatment of purified calpains results in a loss of th ... | 2007 | 17995671 |
| effects of pressure-shift freezing and pressure-assisted thawing on sea bass (dicentrarchus labrax) quality. | effects of pressure-shift freezing and/or pressure-assisted thawing on the quality of sea bass muscle were evaluated and compared with conventional (air-blast) frozen and thawed samples. microstructural analysis showed a marked decrease of muscle cell damage for pressure-assisted frozen samples. according to differential scanning calorimetry (dsc), protein extractability, and sds-page results, high-pressure treatment (200 mpa) produced a partial denaturation with aggregation and insolubilization ... | 2007 | 17995636 |
| mitochondrial metabolism of 17alpha-hydroxyprogesterone in male sea bass (dicentrarchus labrax): a potential target for endocrine disruptors. | the metabolism of 17alpha-hydroxyprogesterone (17p(4)) was investigated in different subcellular fractions isolated from male gonads of sea bass (dicentrarchus labrax l). the existence of cyp17 (c17,20-lyase activity) and cyp11b (11beta-hydroxylase) catalyzed reactions was demonstrated in the mitochondrial fraction, where 17p(4) was converted to androstenedione (ad) and further metabolized to 11beta-hydroxyandrostenedione (betaad). the synthesis of betaad predominated in early spermatogenic test ... | 2007 | 17977610 |
| histo-cytological responses of dicentrarchus labrax (l.) following mercury exposure. | this work deals with the damaging effects of mercury (hg concentrations 251, 355, 501 microgl(-1)) on the structure and ultrastructure of gills, liver, intestine and kidney of farmed european sea bass (dicentrarchus labrax l., 1758) acutely treated for 24 and 48 h. the histoarchitecture of the gills of exposed fish was highly modified due to severe oedema, telangiectasia and secondary lamellar fusion. in hepatocytes and enterocytes hydropic cell swelling, alterations to the endoplasmic reticulum ... | 2008 | 17945343 |
| the effect of environmental salinity on the proteome of the sea bass (dicentrarchus labrax l.). | the european sea bass, dicentrarchus labrax l., tolerates a range of salinities from freshwater to hyper-saline. to study differences in protein expression, fish were reared in both freshwater and seawater. after 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. in all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. fifty differential protein spots were excised f ... | 2007 | 17931403 |
| regulation of progastricsin mrna levels in sea bass (dicentrarchus labrax) in response to fluctuations in food availability. | in this study the sea bass (dicentrarchus labrax) pepsinogen c gene was isolated. the nucleotide sequences of all exons are presented. the organization of the gene is compatible with that of other aspartic proteinases. the predicted 388-residue amino acid (aa) sequence of sea bass pepsinogen c consists of a signal sequence of 16 amino acid residues, an activation peptide of 43 residues, and the mature pepsin of 329 residues containing the two characteristic active-site aspartic acids. we also an ... | 2007 | 17892860 |
| sex hormone-binding globulin expression in sea bass (dicentrarchus labrax l.) throughout development and the reproductive season. | sex hormone-binding globulin (shbg) transports androgens and estrogens in the blood of vertebrate species, including fish, and regulates the bioavailability and metabolic clearance of these steroids. liver is the major site of plasma shbg synthesis, while an shbg homologue, known as the androgen-binding protein, is produced in testes. when shbg gene expression was examined throughout european sea bass (dicentrarchus labrax l.) development, shbg mrna was clearly detectable at 7 days post-fertiliz ... | 2007 | 17719171 |
| quantitative rt-pcr analysis and immunohistochemical localization of hsp70 in sea bass dicentrarchus labrax exposed to transport stress. | in aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (hsps) at the cellular level. in this work we considered the expression of the constitutive and inducible forms of hsp70 as an indicator of stress caused by transport, during development of the sea bass (dicentrarchus labrax), a teleost fish of high value for aquaculture. qualitative rt-pcr analysis revealed expression of inducible hsp70 gene in larvae and fry (25, 40 and 80 days) ... | 2013 | 17664163 |
| juvenile sea bass (dicentrarchus labrax l.) dna strand breaks and lipid peroxidation response following 17beta-estradiol two mode of exposures. | juvenile dicentrarchus labrax l. (sea bass) were exposed to water diluted 17beta-estradiol (e2) (200 ng/l and 2000 ng/l) and treated with intraperitoneal (i.p) injection e2 (0.5 mg/kg and 5 mg/kg) during 10 days in order to study its genotoxicity and peroxidative damage, measured as gill, blood, liver and kidney dna integrity decrease using dna strand breaks assay as well as liver, gill and kidney lipid peroxidation (lpo) respectively. juvenile sea bass gill dna integrity was significantly decre ... | 2008 | 17651802 |
| molecular characterization and central distribution of the estradiol receptor alpha (eralpha) in the sea bass (dicentrarchus labrax). | three different estrogen receptors (ers) have been cloned and characterized in teleosts fish, i.e. eralpha, erbeta or erbeta1 and ergamma or erbeta2. in order to study the sea bass er subtype involved in the regulation of gonadotropin production, as well as to elucidate the possible involved neuronal pathways, we characterized the transactivation properties of the cloned sea bass eralpha (sberalpha) and studied its distribution in the brain and gonadotropic cells of the sea bass by in situ hybri ... | 2008 | 17629451 |
| effect of the pineal hormone melatonin on teleost fish phagocyte innate immune responses after in vitro treatment. | although neuroendocrine-immune system interaction has been shown in teleost fish, no study has evaluated the role of melatonin (mel) on fish immune response even considering that it is affected by the photoperiod. gilthead seabream (sparus aurata l.) and sea bass (dicentrarchus labrax l.) head-kidney leucocytes were incubated with mel (0-control-, 20 pm-400 microm) and leucocyte viability and main innate cellular immune parameters were evaluated. overall, seabream and sea bass head-kidney leucoc ... | 2007 | 17620289 |
| aquaporin molecular characterization in the sea-bass (dicentrarchus labrax): the effect of salinity on aqp1 and aqp3 expression. | euryhaline fish possess the ability to compensate for environmental salinity changes through hydro-mineral regulation. a number of proteins have been studied in order to understand water and ion exchanges, known as fish osmoregulation. sea-bass (dicentrarchus labrax) cdna sequences encoding a homologue of mammalian aquaporin (termed aqp1) and a homologue of mammalian aquaglyceroporin (termed aqp3) have been isolated and sequenced. the aquaporin amino acid sequences share respectively more than 6 ... | 2007 | 17618150 |
| occurrence of two nos isoforms in the developing gut of sea bass dicentrarchus labrax (l.). | in this work we have examined the appearance and distribution of nitric oxide synthase (nos), with histochemical, immunohistochemical and biochemical methods, during development of the sea bass (dicentrarchus labrax) gut. the data showed that both the calcium-calmodulin dependent neuronal isoform (nnos) and calcium-independent inducible isoform (inos) are present in the larval gut of sea bass. the nnos-immunoreactivity was present in the epithelial cells and enteric nerve cells of gut both in th ... | 2007 | 17616932 |
| sea bass ghrelin: molecular cloning and mrna quantification during fasting and refeeding. | ghrelin is a novel appetite-inducing peptide hormone secreted by the stomach. the purpose of this study was first to identify the cdna encoding sequence for ghrelin in sea bass (dicentrarchus labrax). using molecular cloning techniques we sequenced the cdna corresponding to sea bass ghrelin mrna. a total of 798 bases including a 5'-untranslated region (89 bp), an open reading frame (orf) (324 bp), and a 3'-untranslated region (385 bp) were detected. nucleotide sequence (orf) encoded a 108 amino ... | 2008 | 17597623 |
| majority of tcrbeta+ t-lymphocytes located in thymus and midgut of the bony fish, dicentrarchus labrax (l.). | real-time polymerase chain reaction (pcr) and in situ hybridization analyses were performed to investigate the occurrence and distribution of t-lymphocytes expressing tcrbeta in intestine and lymphoid tissues of the bony fish, dicentrarchus labrax (sea bass). immunohistochemistry with the monoclonal antibody dlt15 (pan-t-cell marker) was carried out to compare the cytology, distribution and number of t-cells and tcrbeta+ cells in the various sampled lymphoid organs. the highest tcrbeta expressio ... | 2007 | 17549519 |
| developmental expression of dax1 in the european sea bass, dicentrarchus labrax: lack of evidence for sexual dimorphism during sex differentiation. | dax1 (nr0b1), a member of the nuclear receptors super family, has been shown to be involved in the genetic sex determination and in gonadal differentiation in several vertebrate species. in the aquaculture fish european sea bass, dicentrarchus labrax, and in the generality of fish species, the mechanisms of sex determination and differentiation have not been elucidated. the present study aimed at characterizing the european dax1 gene and its developmental expression at the mrna level. | 2007 | 17537257 |
| compartmentalisation of t cells expressing cd8alpha and tcrbeta in developing thymus of sea bass dicentrarchus labrax (l.). | eggs, larvae, post-larvae and sexually immature juveniles of the teleost dicentrarchus labrax (l.) were assayed for the expression of genes encoding the t cell receptor beta and cd8alpha. rt-pcr of rna extracted from larvae revealed tcrbeta transcripts from day 25 post-hatching (ph) and cd8alpha transcripts from 26 days later. at day 51 ph, cd8alpha and tcrbeta mrnas were localised by in situ hybridisation in thymocytes of the outer and lateral zones of the thymic paired glands. from day 75 ph o ... | 2008 | 17532466 |
| impact of chronic cadmium exposure at environmental dose on escape behaviour in sea bass (dicentrarchus labrax l.; teleostei, moronidae). | the effect of chronic exposure to a low concentration (0.5 microg l(-1)) of cadmium ions was investigated on escape behaviour of sea bass, dicentrarchus labrax, using video analysis. observations were also performed on the microanatomy of lateral system neuromasts. when fish were exposed for 4h per day over 8 days to the cadmium ions, most of both types of neuromasts observed remained intact. however, some of them presented damaged sensory maculae. whereas before cadmium exposure, fish responded ... | 2008 | 17493718 |
| effects of partial substitution of dietary fish oil with blends of vegetable oils, on blood leucocyte fatty acid compositions, immune function and histology in european sea bass (dicentrarchus labrax l). | within a decade or so insufficient fish oil (fo) will be available to meet the requirements for aquaculture growth. consequently, alternative sources are being investigated to reduce reliance on wild fish as a source of fo. vegetable oils (vo) are a feasible alternative to fo. however, it is important to establish that alternative dietary lipids are not only supplied in the correct quantities and balance for optimal growth, but can maintain immune function and prevent infection, since it is know ... | 2007 | 17466094 |
| molecular characterization and seasonal changes in gonadal expression of a thyrotropin receptor in the european sea bass. | the thyroid stimulating hormone (tsh) is a glycoprotein synthesized and secreted from thyrotrophs of the anterior pituitary gland. it acts by binding to and activating its specific receptor, the tshr, to induce the synthesis and secretion of thyroid hormones. recent studies conducted in diverse fish species suggest a direct role of tsh on gonadal physiology. in this work, we describe the cloning of a cdna encoding a tshr which was isolated from the gonads of the european sea bass (dicentrarchus ... | 2007 | 17420017 |
| effect of temperature, ration, body size and age on sulphur isotope fractionation in fish. | sulphur isotope analysis (delta(34)s) is increasingly identified as a valuable tool for source differentiation and the determination of trophic level in food webs, but there are still many uncertainties associated with the interpretation of delta(34)s data. to investigate the effects of temperature, ration, body size and age on sulphur trophic fractionation (deltadelta(34)s) in fish, we reared european sea bass (dicentrarchus labrax) on identical diets at 11 and 16 degrees c at three ration leve ... | 2007 | 17385791 |
| review on the immunology of european sea bass dicentrarchus labrax. | european sea bass (dicentrarchus labrax l.) is a marine species of great economic importance, particularly in mediterranean aquaculture. however, numerous pathogenic viruses, bacteria, fungi and parasites affect the species, causing various infectious diseases and thereby leading to the most heavy losses in aquaculture production of sea bass. in this respect, knowledge on molecular and genetic mechanisms of resistance to pathogens and specific features of immune response against various infectio ... | 2007 | 17382407 |
| cloning, tissue expression, and inducibility of cyp 3a79 from sea bass (dicentrarchus labrax). | multiple members of the cyp3a subfamily have been identified and intensively studied in mammals as they represent prominent cyp enzymes involved in drug metabolism. also in fish, some cyp3a genes have been identified by cdna cloning and immunological techniques, but relatively little is known about their function, distribution, and inducibility. in this study, a novel cyp3a, designated as cyp3a79 was isolated from adult male sea bass, an economically valuable species in fisheries. the sea bass c ... | 2007 | 17366542 |
| organochlorine pesticides and polychlorinated biphenyl residues in reared and wild dicentrarchus labrax from the mediterranean sea (sicily, italy). | the aim of this research was to compare the levels of organochlorine pesticides and pcbs in samples of dicentrarchus labrax living in the straits of messina with samples cultivated in cages in the mediterranean sea. muscles and liver tissues sampled over the months, within the same year, were analyzed. the quantitative determination of the organochlorine compounds was performed by gc-ecd and confirmed with gc-ms. the results showed that the concentrations of ddts in muscles and livers as such of ... | 2007 | 17342439 |
| molecular characterisation of prolactin and analysis of extrapituitary expression in the european sea bass dicentrarchus labrax under various salinity conditions. | although prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. in the mediterranean, the sea bass dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. we characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in d. labrax acclimated to either freshwater or seawa ... | 2007 | 20483280 |
| contribution of dietary arginine to nitrogen utilisation and excretion in juvenile sea bass (dicentrarchus labrax) fed diets differing in protein source. | the role of dietary arginine in affecting nitrogen utilisation and excretion was studied in juvenile european sea bass (dicentrarchus labrax) fed for 72 days with diets differing in protein sources (plant protein-based (pm) and fish-meal-based (fm)). fish growth performance and nitrogen utilisation revealed that dietary arg surplus was beneficial only in pm diets. dietary arg level significantly affected postprandial plasma urea concentrations. hepatic arginase activity increased (p<0.05) in res ... | 2007 | 17321177 |
| cdna sequence and tissue expression of an antimicrobial peptide, dicentracin; a new component of the moronecidin family isolated from head kidney leukocytes of sea bass, dicentrarchus labrax. | a 483-bp cdna was isolated from sea bass (dicentrarchus labrax) head kidney leukocytes, dicentracin, using pcr primers designed from conserved moronecidin domains. gene bank analysis revealed that dicentracin cdna belongs to the moronecidin family. as deduced from alignment with morone chrysops moronecidin, the precursor of 79 aa appeared to be composed of a signal peptide of 22 aa, followed by the mature amp (antimicrobial peptide) of 22 aa named dicentracin, and a c-terminal extension of 35 aa ... | 2007 | 17292649 |
| behavioral and neurophysiological responses of european sea bass groups reared under food constraint. | the individual food-demand behavior of juvenile european sea bass (dicentrarchus labrax, l.) reared in groups under self-feeding conditions was investigated. the triggering activity on self-feeder, i.e. index of the food-demand activity, agonistic interactions and territorial behavior were monitored for periods of 42 to 68 days in six groups of 50 fish. the specific growth rate was calculated and the brain serotonergic activity was used as a stable index of social stress. inter-individual differ ... | 2007 | 17188721 |
| cellular alterations in different organs of european sea bass dicentrarchus labrax (l.) exposed to cadmium. | specimens of farmed european sea bass (dicentrarchus labrax l., 1758) were exposed to different cadmium (cd) concentrations (4.47, 5.63, 7.08 and 8.91 mg l(-1)) for 24 and 48 h. the effects of cd on numbers of some cell types and structures (i.e., chloride cells, ccs; macrophage aggregates, mas; rodlet cells, rcs) and on structure and ultrastructure of the main organs (gill, liver, intestine, kidney) were studied with routine process for light and transmission electron microscopy. following cadm ... | 2007 | 17188326 |