| integration of avian sarcoma virus dna sequences in transformed mammalian cells. | dna from six avian sarcoma virus (asv)-transformed mammalian cell lines was digested with the restriction endonucleases ecori, xho i, or sal i, fractionated by agarose gel electrophoresis, transferred to nitrocellulose filter strips, and hybridized with specific asv [32p]cdna probes. dna from all of the asv-transformed cell lines yielded three common virus-specific dna fragments (2.4, 1.8, and 1.3 x 10(6) daltons) upon cleavage with ecori. xho i appeared to cleave at least once within the integr ... | 1977 | 200912 |
| phosphorylated and nonphosphorylated forms of avian sarcoma virus polypeptide p19. | | 1977 | 195394 |
| cellular functions are required for the synthesis and integration of avian sarcoma virus-specific dna. | | 1977 | 196759 |
| terminally repeated sequences in the avian sarcoma virus rna genome. | the initiation of dna synthesis in vitro by rna-directed dna polymerase (deoxynucleosidetriphosphate: dna deoxynucleotidyltransferase, ec 2.7.7.7) of avian oncornaviruses requires a trnatrp primer molecule located close to the 5' end of the viral rna genome. dna transcripts, 100 nucleotides in length, initiated on the trnatrp primer molecule contain nucleotide sequences complementary to a large (25 nucleotides) rnase t1 oligonucleotide, t-13, located at the 5' terminus of the avian sarcoma virus ... | 1977 | 70037 |
| the beta subunit of the dna polymerase of avian sarcoma virus strain b77 is a phosphoprotein. | | 1977 | 68618 |
| nucleotide sequence at the 5' terminus of the avian sarcoma virus genome. | transcription of dna from the rna genome of avian sarcoma virus by rna-directed dna polymerase in vitro initiates on a primer (trnatrp) located near the 5'-terminus of the viral genome. one of the major products of transcription is a single-stranded dna chain complementary to a sequence of 101 nucleotides immediately distal to the site of initiation of dna synthesis. we have determined the complete nucleotide sequence of this transcribed chain for the prague strain of avian sarcoma virus, a part ... | 1977 | 67601 |
| sequence specificity of internal methylation in b77 avian sarcoma virus rna subunits. | following ribonuclease digestion of methyl-3h-labeled b77 avian sarcoma virus rna subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. in addition to methylated nucleosides in the 5'-terminal cap structure, m7g(5')gmpcp, n6-methyladenosine(m6a) was found to be present in only two internal sequences of the rna molecule, gpm6apc and apm6apc. the averag ... | 1977 | 189800 |
| influence of defective virion core proteins on rna maturation with an avian sarcoma virus. | the rna of the avian sarcoma virus b77 temperature-sensitive mutant la334 was investigated using electrophoretic analysis. the rna from mutant virus grown at the nonpermissive temperature (42degrees c) showed a heterogeneous peak between 80 and 125s, and another at about 35s. the rna of the mutant virus grown at the permissive temperature (35 degrees c) behaved like wild-type b77 virus rna, exhibiting a major peak at 70s. the homology between the various rna fractions and virus-specific dna prob ... | 1977 | 189076 |
| nucleotide sequence that binds primer for dna synthesis to the avian sarcoma virus genome. | initiation of transcription from the genome of avian sarcoma virus by rna-directed dna polymerase in vitro requires trnatrp as a primer. the trna is bound to the viral genome by a sequence of 16 contiguous nucleotides (u-c-a-c-g-u-c-g-g-g-g-u-c-a-c-cp), beginning with the penultimate base at the 3' terminus of the primer and extending through the acceptor stem into loop iv of the trna. consequently, the native conformation of the trna must be disrupted by the binding of primer to the viral genom ... | 1976 | 183013 |
| blocked, methylated 5'-terminal sequence in avian sarcoma virus rna. | | 1975 | 170541 |
| site on the rna of an avian sarcoma virus at which primer is bound. | in vitro transcription of the avian tumor virus rna by rna-directed dna polymerase is initiated on the unique cellular 4s rna. previous studies have shown that on the average there is one such rna primer hydrogen bonded to each viral 35s rna. the present study confirms that finding and demonstrates that, at least for the majority of 35s rna molecules, the primer is bound at a site close to the 5'-terminus. | 1975 | 169390 |
| reassociation of 4 s and 5 s rna's with the genome of avian sarcoma virus. | | 1975 | 49977 |
| accumulation of water during transformation of cells by an avian sarcoma virus. | | 1974 | 4373174 |
| complementation of transforming functions by temperature-sensitive mutants of avian sarcoma virus. | | 1973 | 4351612 |