| determination of the coding capacity of the bamhi dna fragment b of apathogenic herpes simplex virus type 1 strain hfem by dna nucleotide sequence analysis. | herpes simplex virus type 1 (hsv-1) strain hfem acquired an apathogenic phenotype due to a deletion within the dna sequences of the bamhi dna fragment b of the viral genome. in order to investigate the coding strategy of this particular region of the genome of hsv-1 strain hfem the dna nucleotide sequence of the bamhi dna fragment b was determined. this analysis revealed that the bamhi dna fragment b of hsv-1 strain hfem comprises 6593 bp, corresponding to the nucleotide positions (np) 113322 to ... | 1992 | 1332274 |
| heparan sulfate glycosaminoglycans as primary cell surface receptors for herpes simplex virus. | our current incomplete picture of the earliest events in hsv infection may be summarized as follows. the initial interaction of virus with cells is the binding of virion gc to heparan sulfate moieties of cell surface proteoglycans. stable binding of virus to cells may require the interaction of other virion glycoproteins with other cell surface receptors as well (including the interaction of gb with heparan sulfate). penetration of virus into the cell is mediated by fusion of the virion envelope ... | 1992 | 1332443 |
| effects of dimethyl prostaglandin a1 on herpes simplex virus and human immunodeficiency virus replication. | we have investigated the direct effect of dimethyl prostaglandin a1 (dmpga1) on the replication of herpes simplex virus (hsv) and human immunodeficiency virus type 1 (hiv-1). dmpga1 significantly inhibited viral replication in both hsv and hiv infection systems at concentrations of dmpga1 that did not adversely alter cellular dna synthesis. the 50% inhibitory concentration (id50) for several hsv type 1 (hsv-1) strains ranged from 3.8 to 5.6 micrograms/ml for vero cells and from 4.6 to 7.3 microg ... | 1992 | 1332592 |
| improved synthesis and biological evaluation of an acyclic thiosangivamycin active against human cytomegalovirus. | we previously described the synthesis and in vitro antiviral activity of an acyclic thiosangivamycin analog (gupta et al., 1989a). in order to extend these initial studies, a new, multi-gram synthesis of 4-amino-7-[(2-hydroxy- ethoxy)methyl]pyrrolo]2,3-d]pyrimidine-5-thiocarboxamide (compound 229) was achieved in 5 steps from the known 5-amino-2-bromo-3,4-dicyanopyrrole in good overall yield. in plaque reduction assays with hcmv, compound 229 had an ic50 of 7 microm; in yield reduction assays th ... | 1992 | 1332597 |
| the hiv replication inhibitor 3'-fluoro-3'-deoxythymidine blocks sialylation of n-linked oligosaccharides. | the ability of 3'-fluoro-3'-deoxythymidine (flt) to interfere with glycosylation was investigated in an experimental system, where the effects on the herpes simplex virus type 1-specified glycoprotein gc were determined. by adding flt to hsv-infected cells after the peak of dna synthesis, it was possible to segregate possible effects on nucleic acid metabolism from the effects on glycosylation of gc. it was found that flt treatment of hsv-infected cells at concentrations of 20-500 micrograms/ml ... | 1992 | 1332599 |
| effect of (s)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine on the replication and morphogenesis of herpes simplex virus type 1. | treatment of african green monkey kidney cells with 1 microgram/ml of (s)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (hpmpc) inhibited the release of infectious herpes simplex virus type 1 (hsv-1) by more than 90%. electron microscopic observations of hpmpc-treated monkey kidney cells demonstrated few intracellular or extracellular viral particles. the viral particles seen were without dense cores. in addition, hpmpc blocked cell fusion induced by hsv-1 in monkey kidney cells. immunobl ... | 1992 | 1332603 |
| rapid detection of human herpes simplex virus type 1 in saliva. | herpes simplex virus type 1 (hsv1) can be rapidly identified in saliva from patients with acute herpetic gingivostomatitis, by in vitro amplification using the polymerase chain reaction and specific primers. amplification of dna results in a product of 110 bp length corresponding to the region 1381-1490 bp of the hsv1 thymidine kinase gene. the specificity of the reaction was demonstrated in three ways: (i) the presence of a sma 1 restriction enzyme site in the amplified product sequence; (ii) s ... | 1992 | 1332660 |
| [effects of nitroxyl radicals on pathogenic properties of herpesvirus (the spin-label technique in the study of virus-macrophage interactions)]. | the effect of various concentrations of both methyl ether of 5-doxyl-stearic acid (m5ds) and 4-maleimido-tempo (4mt) on the pathogenicity of herpes simplex virus (hsv-1) was studied. it is known that the reagents modify the lipid matrix and the proteins of virion envelope. the decrease of the hsv-1 pathogenicity was shown when using the concentration of reagents more 5 x 10(-5) m. hsv-1 having high pathogenicity and cytotoxicity was obtained when the concentrations of the reagents were less 5 x ... | 1992 | 1332789 |
| transdominant inhibition of herpes simplex virus growth in transgenic mice. | a mutant allele (x25) of an essential regulatory protein, icp4, encoded by herpes simplex virus (hsv) has been shown to have a transdominant, negative effect on the activity of the wild-type protein, resulting in the inhibition of virus growth in vitro. the x25 protein appears to exert its transdominant effect by sequestering functional icp4 monomers into nonfunctional, heterodimeric complexes (a. shepard, p. tolentino, and n. a. deluca, 1990, j. virol. 64, 3916-3926). in order to assess the ant ... | 1992 | 1333114 |
| identification and transcriptional mapping of genes encoded at the ir/us junction of equine herpesvirus type 1. | two open reading frames (orfs) encoded at the inverted repeat unique short (us) junction of the short (s) region of the equine herpesvirus type 1 genome were identified by dna sequencing of a 2876 base pair (bp) genomic segment, and transcripts encoding these orfs were characterized by northern blot, s1 nuclease, and primer extension analyses. these studies also established the size of each inverted repeat to be 12,768 nucleotides (nts). the ir6 orf (816 bp), mapping at nts 12,317-11,502 of the ... | 1992 | 1333117 |
| the virulence-determining genomic bamhi fragment 4 of pseudorabies virus contains genes corresponding to the ul15 (partial), ul18, ul19, ul20, and ul21 genes of herpes simplex virus and a putative origin of replication. | the genomic bamhi-fragment 4 of pseudorabies virus (prv) has previously been shown to encode functions necessary for expression of prv neurovirulence (b. lomniczi, s. watanabe, t. ben-porat, and a. s. kaplan, 1984, j. virol. 52, 198-205). to identify proteins that might be involved in the neurotropism of prv we sequenced the complete 9382-bp fragment bamhi-4, the longest contiguous sequence determined in the ul region of prv so far, and analyzed its coding capacity. in an arrangement similar to ... | 1992 | 1333128 |
| the latency-related gene of bovine herpesvirus 1 inhibits the activity of immediate-early transcription unit 1. | bovine herpesvirus 1 (bhv-1) establishes a latent infection in sensory neurons of infected animals. only one virus-encoded latency-related (lr) gene is expressed during a latent infection. the lr transcript overlaps immediate-early transcription unit 1 (ietu1) and is anti-sense with respect to ietu1. the transcriptional start site of the lr rna was mapped to position 724 of the lr gene, downstream from two putative tata elements. when lr promoter sequences were deleted from a plasmid containing ... | 1992 | 1333133 |
| effects of acyclovir, oxetanocin-g, and carbocyclic oxetanocin-g in combinations on the replications of herpes simplex virus type 1 and type 2 in vero cells. | 9-(2-hydroxyethoxymethyl)guanine (acyclovir, acv) and novel nucleosides, 9-(2-deoxy-2-hydroxymethyl-beta-d-erythro-oxetanocyl)guanine (oxetanocin-g, oxt-g) and (+)-9-[(1r, 2r, 3s)-2, 3-bis(hydroxymethyl)cyclobutyl]guanine (carbocyclic oxetanocin-g, carbocyclic oxt-g) possessed substantial antiviral activities against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). acv inhibited only viral thymidine kinase positive (tk+) herpes viruses, although the latter two compounds inhibited the repl ... | 1992 | 1333651 |
| factors influencing the interaction of herpes simplex virus glycoprotein c with the third component of complement. | the factors influencing the interaction of herpes simplex virus (hsv) glycoprotein c (gc) with the third component of complement (c3) were investigated in this study. the ability of gc of hsv type 1 (gc-1) to bind to the c3b fragment of c3 was found to be influenced by cell specific processing of gc-1 in a different manner, binding being remarkably enhanced in some cell lines following removal of sialic acid residues. testing several intertypic recombinants of hsv we found that only strains expr ... | 1992 | 1333754 |
| identification and nucleotide sequence of a gene in feline herpesvirus type 1 homologous to the herpes simplex virus gene encoding the glycoprotein b. | the nucleotide sequence of the glycoprotein b (gb) homologous gene of feline herpesvirus type 1 (fhv-1) was determined. the gene was found to be located within a 9.6 kbp sali fragment by southern-blot hybridization with a probe derived from the herpes simplex virus type 1 (hsv-1) gb dna sequence. furthermore, the predominant portion of the coding sequences was mapped to a 1.9 kbp hin cii-ecori and its flanking 2.7 kbp eco ri-eco ri subfragments in the 9.6 kbp sali fragment. the entire nucleotide ... | 1992 | 1333759 |
| temperature sensitivity of herpes simplex virus type 1 is a tissue-dependent phenomenon. | the temperature sensitivity of herpes simplex virus type 1 (hsv-1) was assessed in primary cultures of mouse central nervous system (mns) cells and mouse embryo cells (mec). infectious yields were determined and the ultrastructural morphogenesis of hsv-1 particles was compared following incubation at 37 or 40.5 degrees c. yields of infectious virus were significantly reduced for both types of cell cultures following incubation at 40.5 degrees c. however, the effect of supraoptimal temperature (4 ... | 1992 | 1333760 |
| binding of surfactant protein a (sp-a) to herpes simplex virus type 1-infected cells is mediated by the carbohydrate moiety of sp-a. | pulmonary surfactant protein a (sp-a) has been shown to act as an opsonin in the phagocytosis of viruses by alveolar macrophages. to determine whether sp-a binds to viral proteins and which part of the sp-a molecule is involved in this interaction, binding studies were undertaken. sp-a was labeled with fluorescein isothiocyanate, and its binding to herpes simplex virus type 1-infected hep-2 cells, as a model for virus-infected cells in general, was studied using flow cytometry. the binding of sp ... | 1992 | 1334078 |
| ocular involvement in an outbreak of herpes gladiatorum. | an epidemic of herpes simplex virus type 1 occurred in 60 of 175 wrestlers (34%) attending a four-week intensive training camp. five of these 60 patients (8%) developed ocular involvement that included follicular conjunctivitis, blepharitis, and phlyctenular disease. cultures of the conjunctiva and eyelid vesicles were positive for herpes simplex virus type 1 in four of the five patients with ocular disease. the viral isolates were compared by restriction-endonuclease analysis, which disclosed t ... | 1992 | 1334374 |
| inhibition of herpes simplex virus replication and protein synthesis by non-smoked tobacco, tobacco alkaloids and nitrosamines. | inhibitory effects of snuff extract and the tobacco chemicals nicotine, anabasine, diethyl-n-nitrosamine (den), and the tobacco-specific nitrosamines (tsna), n-nitrosonornicotine (nnn) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) on herpes simplex virus type 1 (hsv-1) replication in vitro and on hsv-1 protein synthesis in infected cells were analysed. snuff extract and nicotine caused a significant reduction of hsv-1 attachment to cell membranes whereas anabasine, den, nnn and nnk di ... | 1992 | 1334651 |
| gene therapy of malignant brain tumors: a rat glioma line bearing the herpes simplex virus type 1-thymidine kinase gene and wild type retrovirus kills other tumor cells. | tumor cells infected with a retrovirus vector (vik) containing the herpes simplex virus thymidine kinase (hsv-tk) gene can be selectively killed by treatment with nucleoside analogues, such as ganciclovir. to mediate delivery of the hsv-tk gene to "recipient" tumor cells, "donor" c6 rat glioma cells infected with the vik vector (c6vik) were superinfected with wild type moloney murine leukemia virus (wt mo-mlv). these modified donor cells (c6vikwt) produced both wild type retrovirus and the vik v ... | 1992 | 1335091 |
| the ir3 gene of equine herpesvirus type 1: a unique gene regulated by sequences within the intron of the immediate-early gene. | the complete nucleotide sequence of the inverted repeat component (ir; 12,776 bp each) of the genome of equine herpesvirus type 1 (ehv-1) has been determined. transcription analyses have revealed that the ehv-1 ir sequence encodes at least 6 genes. in this report, we present the dna sequence and transcriptional characterization of a gene (ir3) that maps entirely within the ir sequences. the ir3 open reading frame (orf) is located between nucleotides (nt) 6123-6411 of the ir sequence and possesse ... | 1992 | 1335300 |
| latent infection of scid mice with herpes simplex virus 1 and lethal cutaneous lesions in pregnancy. | some scid mice survived primary infection with herpes simplex virus 1 without the development of peripheral lesions but established coculture-positive ganglionic latency when a low dose of a wild-type strain was inoculated intracutaneously. the latency was also evidenced by the development of the fatal zosteriform skin lesions and the isolation of the virus during pregnancy. we consider that the viral entry into neurons without successive replication, rather than the arrest of the lytic infectio ... | 1992 | 1335542 |
| replication inhibition by nucleoside analogues of a recombinant autographa californica multicapsid nuclear polyhedrosis virus harboring the herpes thymidine kinase gene driven by the ie-1(0) promoter: a new way to select recombinant baculoviruses. | the expression of the thymidine-thymidylate kinase (hsv1-tk), (atp: thymidine 5'-phosphotransferase; ec 2.7.1.21) of herpes simplex virus type 1 endows the host cell with a conditional lethal phenotype which depends on the presence of nucleoside analogues metabolized by this enzyme into toxic inhibitors of dna replication. to generate a recombinant baculovirus that could be selected against by nucleoside analogs, the hsv1-tk coding sequence was placed under the control of the autographa californ ... | 1992 | 1335569 |
| the herpes simplex virus type 1 icp6 gene is regulated by a 'leaky' early promoter. | expression from the promoter for the large subunit (icp6) of the ribonucleotide reductase encoded by herpes simplex virus type 1 (hsv-1) has been examined. using the lacz reporter gene fused in-frame with icp6 regulatory sequences to assay expression quantitatively, we showed that the icp6 promoter responded very weakly to the alpha-transinducing factor (tif) in the absence of all other viral gene products, but much more strongly to immediate early proteins. similar patterns of regulation were o ... | 1992 | 1335673 |
| mutations in the ul53 gene of hsv-1 abolish virus neurovirulence to mice by the intracerebral route of infection. | the cell fusion protein, the product of the ul53 gene, is responsible for intracerebral (ic) pathogenicity of hsv-1. recombinant hsv-1 r15 is apathogenic to mice by the ic route of inoculation, while intratypic recombinants, in which the ul53 gene in r15 was replaced by an analogous sequence from the pathogenic strain r19, regained ic pathogenicity. the nucleotide sequence of the ul53 gene of hsv-1 strains r15 (apathogenic) and r19 (pathogenic) was determined and compared to that of other pathog ... | 1992 | 1335675 |
| herpes simplex-1 virus thymidine kinase gene is unable to completely eliminate live, nonimmunogenic tumor cell vaccines. | recent experiments with genetically engineered tumors have generated renewed interest in active cellular immunotherapy as a cancer treatment modality. in order to consider the use of live tumor cells for immunotherapy in human cancer patients, it will be important to ensure that these cells do not themselves produce morbidity in the event the immune system fails to eliminate them. toward this end, we have examined a strategy for eliminating genetically manipulated nonimmunogenic tumors in vivo. ... | 1992 | 1335754 |
| detection of latent herpes simplex virus dna and rna in human geniculate ganglia by the polymerase chain reaction. | by using the polymerase chain reaction (pcr) we detected latent herpes simplex virus type 1 (hsv-1) in human geniculate and trigeminal ganglia obtained from autopsy cases. a pair of primers which were specific for a part of the hsv-1 thymidine kinase domain were used for detection of hsv dna. we also examined the latency-associated transcript (lat), known as latency-specific rna, by means of reverse transcription-pcr with a pair of lat-specific primers. hsv-1 dna was detected in 16 of 17 (94%) t ... | 1992 | 1336296 |
| mode of antiviral action of penciclovir in mrc-5 cells infected with herpes simplex virus type 1 (hsv-1), hsv-2, and varicella-zoster virus. | the metabolism and mode of action of penciclovir [9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine; brl 39123] were studied and compared with those of acyclovir. in uninfected mrc-5 cells, low concentrations of the triphosphates of penciclovir and acyclovir were occasionally just detectable, the limit of detection being about 1 pmol/10(6) cells. in contrast, in cells infected with either herpes simplex virus type 2 (hsv-2) or varicella-zoster virus (vzv), penciclovir was phosphorylated quickly to gi ... | 1992 | 1336346 |
| herpes simplex virus types 1 and 2 and cytomegalovirus in peptic ulcer disease and non-ulcer dyspepsia. | the prevalence and the serum levels of igg antibody to herpes simplex virus type 1 or 2 (hsv1, hsv2) and to cytomegalovirus (cmv) were studied by elisa in patients with active peptic ulcer -- duodenal and gastric -- and non-ulcer dyspepsia. two hundred and forty-two consecutive patients with endoscopically confirmed active peptide ulcer -- 170 duodenal ulcers, 72 gastric ulcers -- and 95 consecutive patients who fulfilled the criteria for the diagnosis of non-ulcer dyspepsia were included in the ... | 1992 | 1336474 |
| transneuronal transfer of herpes simplex virus type 1 (hsv 1) from mixed limb nerves to the cns. i. sequence of transfer from sensory, motor, and sympathetic nerve fibres to the spinal cord. | the time course of transneuronal transfer of herpes simplex virus type 1 (hsv 1) from sensory, motor, and sympathetic nerve fibres to connected spinal neurones was examined. after injection of a constant number of infectious units into distal forelimb or hindlimb nerves of inbred rats of the same age, the extent of viral transfer was strictly dependent on the survival time postinoculation (p.i.). retrograde transport to somatic motoneurones occurred at 28-29 hours p.i. (stage 1), in synchrony wi ... | 1992 | 1336502 |
| search for evidence of herpes simplex virus, type 1, or varicella-zoster virus infection in postmortem brain tissue from schizophrenic patients. | the highly sensitive polymerase chain reaction (pcr) was used to search for herpes simplex virus type 1 (hsv-i) or varicella-zoster virus (vzv) in the dna extracted from postmortem temporal cortex samples of 8 schizophrenic subjects, 8 nonschizophrenic suicide victims and 8 normal controls. hsv-i or vzv-specific dna amplification was not detected in any of the samples studied. | 1992 | 1336636 |
| pcr-mediated search for herpes simplex virus dna in sections of brain from patients with multiple sclerosis and other neurological disorders. | herpes simplex virus (hsv) has been shown to cause central nervous system demyelination in experimental animals and several studies have implicated hsv in the aetiology of multiple sclerosis (ms). we have used the polymerase chain reaction to look for dna of both type 1 hsv (hsv-1) and type 2 hsv (hsv-2) in formalin-fixed paraffin-embedded brain tissues from patients with ms and other neurological diseases. primers which amplify a fragment of the normal cellular gene c-myc were included in the r ... | 1992 | 1336795 |
| ménière's disease and antibody reactivity to herpes simplex virus type 1 polypeptides. | it has been reported that the inner ear is capable of responding to antigen challenge. in this study, we have investigated the antibody reactivity to herpes simplex virus 1 (hsv-1) proteins in sera from patients with ménière's disease. | 1992 | 1337422 |
| specificity of antibody responses affected by extracorporeal immunoadsorption of plasma over columns of protein a silica. | a relationship is described between the interaction of circulating immune complexes (cic) from plasma with staphylococcal protein a immunoadsorption treatment columns and modulation of antibody responses related to the specific cic. eluates from the initial immunoadsorption columns used to treat a series of patients with breast adenocarcinoma, cancer chemotherapy-associated thrombotic thrombocytopenic purpura/hemolytic uremic syndrome (c-ttp/hus), or immune thrombocytopenic purpura (itp) were ev ... | 1992 | 1337544 |
| isolation of herpes simplex virus-1 by intracellular membranes of bhk tk- cells. | baby hamster kidney (bhk tk-) cells infected with herpes simplex virus-1 (hsv-1) showed a large number of virus particles isolated in vesicles characterized by the presence or the absence of ribosomes or inside cisternae of the rough endoplasmic reticulum or the nuclear envelope. the isolation of the virions by intracellular membranes appeared shortly after infection of the cells by hsv-1. these structures persisted for longer periods where no morphological alterations in the infected cells were ... | 1992 | 1337570 |
| [herpesvirus research at the virology institute of the slovak academy of science]. | research of the herpes virus (hsv) in the institute of virology of the slovak academy of sciences in bratislava has achieved some interesting results. an original technique has been elaborated for the purification of hsv visions and the separation of envelopes and naked particles. studies of the properties of the hszp strain of hsv type 1, adapted by us, showed that this virus did not only fail to shut off host cell proteosynthesis but also interfered with this ability of the kos strain. interes ... | 1992 | 1337712 |
| the diagnosis of recent herpes simplex virus type 2 genital infections by the simplex-2 test. | the prevalence of complement-fixing (cf) antibody against the ag-4 early antigen of herpes simplex virus (hsv) type 2 (hsv-2) was determined in patients with culture confirmed hsv-2 genital herpes and control groups using a commercial hsv-2 early antigen (simplex-2; gene link australia ltd). eighty seven per cent of 39 sera collected between 14 and 28 days after confirmed primary and recurrent hsv-2 infection were positive. in acute sera collected between 2-10 days after onset the simplex-2 test ... | 1992 | 1337770 |
| uracil in oris of herpes simplex 1 alters its specific recognition by origin binding protein (obp): does virus induced uracil-dna glycosylase play a key role in viral reactivation and replication? | we have recently demonstrated that mammalian uracil-dna glycosylase activity is undetectable in adult neurons. on the basis of this finding we hypothesized that uracil, derived either from oxidative deamination of cytosine or misincorporation of dump in place of dtmp during dna repair by the unique nuclear dna polymerase present in adult neurons, dna polymerase beta, might accumulate in neuronal dna. uracil residues could also arise in the herpes simplex 1 (hsv1) genome during latency in nerve c ... | 1992 | 1337882 |
| a sequence-specific dna-binding protein recognising a ga-rich element cooperates with oct-1 at the herpes simplex virus type 1 ie3 promoter. | herpes simplex virus immediate early (ie) gene transcription is regulated via the upstream taatgarat motif and involves the recruitment of the ubiquitous octamer-binding protein oct-1 and a component of the virion vmw65 into a multiprotein complex termed the immediate early complex (iec). an upstream ga-rich element (ga-re) has been postulated to mediate a response to vmw65 independent of taatgarat. we have studied iec formation in gel shift assays using dna fragments containing these sequence e ... | 1992 | 1338062 |
| synergistic effect of flavones and flavonols against herpes simplex virus type 1 in cell culture. comparison with the antiviral activity of propolis. | the in vitro activity against herpes simplex virus type 1 of the major flavonoids identified in propolis was investigated. flavonols were found to be more active than flavones, the order of importance being galangin, kaempferol, and quercetin. the efficacy against hsv-1 of binary flavone-flavonol combinations has been also investigated. the synergy demonstrated by all combinations could explain why propolis is more active than its individual compounds. | 1992 | 1338212 |
| [treatment of experimental liver tumors by in vivo suicide gene transfer in rats]. | we investigated whether the transduction of a suicide gene might induce the elimination of malignant solid tumours. bdix male rats were given an intra-hepatic injection of a mixture containing dhdk12 tumor cells and xenogeneic fibroblasts. the latter were producing either the herpes simplex virus type 1 thymidine kinase hsv1-tk- or nls-lac z(control)-expressing recombinant retroviral particles. 5 days later, a time at which the tumor is macroscopic, all the rats were treated with ganciclovir, a ... | 1992 | 1338622 |
| influence of t-actemodulin on herpes simplex virus infection. | the aim of the investigation was to study the direct influence of t-actemodulin (tam) on the herpes simplex virus (hsv) infection in vitro and in vivo. hsv-1 (strain vic) and hsv-2 (strain nissa) were used in the experiments in vitro. the experiments were performed using multicycle and singlecycle growth tests. seventy-five patients suffering from hsv infections (labialis and genitalis) were locally treated by 0.04% tam-containing hydrophilic gel or water-washable cream (applied every 3 h). the ... | 1992 | 1339076 |
| recurrent genital infection in the guinea pig: differences between herpes simplex types 1 and 2. | recurrence rates of genital infections are significantly higher for herpes simplex virus (hsv) type 2 than hsv type 1. reasons for this difference are not known. in this report, multiple strains of hsv-1 and hsv-2 were evaluated in the guinea-pig model. hsv-2 strains showed significantly higher genital lesion recurrence than hsv-1, including hsv-1 mckrae strain which is highly recurrent in ocular infections. hsv-2 strains were also associated with more frequent asymptomatic vaginal virus sheddin ... | 1992 | 1339184 |
| [herpes simplex virus pneumonia following transplantation]. | the hsv (1 or 2) is the cause of serious pulmonary infections among patients who have had a transplantation. this study in retrospect is based on the analysis of 145 patients who underwent a cardiothoracic transplant at the chu. in nancy. confronted with clinical signs calling to mind breathing difficulties, the analysis of the broncho alveolar lavage (or of the bronchial brushing) revealed the viral aetiological agent. the answer from the laboratory is quickly available by immunofluorescence or ... | 1992 | 1340110 |
| clinical relevance of herpes simplex virus in the throat of elderly with salmonella enteritidis gastroenteritis. | it is not known whether the presence of herpes simplex virus in the throat of elderly patients with severe gastroenteritis and pulmonary implications is of clinical relevance. we cultured throat swabs and faeces of elderly patients with (n = 11) and without (n = 12) severe salmonella gastroenteritis for viruses and bacteria to study the aetiology of respiratory complications. complement fixation titers for anti-herpes simplex antibodies in paired sera were also ascertained. throat swabs of 6 out ... | 1992 | 1341452 |
| mollaret's meningitis and herpes simplex virus type 1. | | 1992 | 1345412 |
| selective killing of cd4+ cells harboring a human immunodeficiency virus-inducible suicide gene prevents viral spread in an infected cell population. | we have stably expressed in cd4+ lymphoid cells the herpes simplex virus type 1 thymidine kinase (hsv1-tk) gene under the control of the human immunodeficiency virus (hiv) promoter and transactivation response element sequences. upon hiv infection these regulatory sequences were transactivated, switching on high-level expression of hsv1-tk. this in turn caused the death of hiv-infected cells when they were cultured in the presence of acyclovir, a nucleoside analog that becomes toxic after phosph ... | 1992 | 1346066 |
| phenotypic and functional studies on ocular t cells during herpetic infections of the eye. | herpetic stromal keratitis an inflammatory disease of the eye resulting from herpes simplex virus type 1 infection, is a common cause of blindness. the disease is generally considered to represent an immunopathologic response, but the exact mechanism remains in doubt and is subject to debate. we have investigated the nature of inflammatory cells in the eye and have isolated ocular cells to establish their phenotype and determine some of their functions. by means of immunocytochemistry and cytofl ... | 1992 | 1347309 |
| establishment and functional characterization of human herpesvirus 6-specific cd4+ human t-cell clones. | in order to clarify the protective immune responses against a newly identified herpesvirus, human herpesvirus 6 (hhv-6), we established hhv-6-specific human t-cell clones and examined their functional properties. five cd3+cd4+cd8- t-cell clones, which proliferated in response to stimulation with two different strains of hhv-6 in the presence of autologous antigen-presenting cells but not with herpes simplex virus type 1 or human cytomegalovirus, were established from peripheral blood lymphocytes ... | 1992 | 1348547 |
| projections from rabbit caudal medulla to c1 and a5 sympathetic premotor neurons, demonstrated with phaseolus leucoagglutinin and herpes simplex virus. | we combined phaseolus vulgaris leucoagglutinin anterograde tracing and herpes simplex virus transneuronal retrograde tracing to determine whether neurons in the vasodepressor region of the rabbit caudal ventrolateral medulla project to brainstem neurons containing the virus after its transneuronal transport from the adrenal medulla. five days after adrenal injection of virus, 764 +/- 159 virus-positive neurons were found bilaterally in the brainstem: 61% in the c1 sympathoexcitatory region of th ... | 1992 | 1349616 |
| herpetic stromal keratitis: an immunopathologic disease mediated by cd4+ t lymphocytes. | to investigate the role of t cell subsets in the development of herpetic stromal keratitis (hsk) in a well defined model, we used an adoptive transfer approach in which thymectomized and t cell-depleted mice [t(-)] were reconstituted with different numbers of syngeneic immune t lymphocytes after topical corneal challenge with re strain of herpes simplex virus-1. in vitro stimulated or unstimulated immune t cells obtained from cervical and retropharyngeal lymph nodes of mice with hsk were used in ... | 1992 | 1351475 |
| inhibition of viral and cellular promoters by human wild-type p53. | mutation of the p53 tumor suppressor gene is a recurring event in a variety of human cancers. wild-type p53 may regulate cell proliferation and has recently been shown to repress transcription from several cellular promoters. we studied the effects of wild-type and mutant human p53 on the human proliferating-cell nuclear antigen promoter and on several viral promoters including the simian virus 40 early promoter-enhancer, the herpes simplex virus type 1 thymidine kinase and ul9 promoters, the hu ... | 1992 | 1352831 |
| activation of human immunodeficiency virus by herpes simplex virus. | heterologous viruses have been examined for their ability to accelerate the course of infection with the human immunodeficiency virus (hiv) type 1. in this study, ach-2 cells persistently infected with hiv-1 exhibited augmented hiv-1 replication as a result of superinfection with herpes simplex virus (hsv) type 1. using hsv-1 mutants with deletions in the genes encoding immediate-early proteins icp0, icp4, and icp27, it was found that icp0 and icp27, but not icp4, were essential for up-regulatio ... | 1992 | 1354237 |
| novel thiosemicarbazones derived from formyl- and acyldiazines: synthesis, effects on cell proliferation, and synergism with antiviral agents. | the synthesis of a series of novel thiosemicarbazones (tsc's) derived from various alkyl diazinyl (3-pyridazinyl, 4-pyrimidinyl, 2-pyrazinyl) ketones and 3-pyridazinecarbaldehyde and their evaluation against herpes simplex virus (hsv) and human immunodeficiency virus (hiv) as well as the determination of their cytotoxicity are described. in addition, the effects of combination of such tsc's with the well-known antiviral drugs acyclovir (acv) and 3'-azido-3'-deoxythymidine (azt) were studied. und ... | 1992 | 1354751 |
| modulation of cellular and viral promoters by mutant human p53 proteins found in tumor cells. | wild-type p53 has recently been shown to repress transcription from several cellular and viral promoters. since p53 mutations are the most frequently reported genetic defects in human cancers, it becomes important to study the effects of mutations of p53 on promoter functions. we, therefore, have studied the effects of wild-type and mutant human p53 on the human proliferating-cell nuclear antigen (pcna) promoter and on several viral promoters, including the herpes simplex virus type 1 ul9 promot ... | 1992 | 1356162 |
| recurrent hsv-1 virus infection complicated with recalcitrant headache treated with acyclovir. | | 1992 | 1357873 |
| on the antiviral activity of diffusomycin (oxazolomycin). | the effect of the beta-lactone antibiotic diffusomycin (oxazolomycin) was investigated against vaccinia (lister), herpes simplex type 1 (kupka), influenza a (wsn; h1n1), and coxsackie a9 viruses. diffusomycin reduced significantly the plaque formation of enveloped dna and rna viruses by more than 90% in the range of the maximally tolerated dose. as could be shown with vaccinia virus, the antiviral action was not caused by virucidal effect on virions or by interaction with virus adsorption and pe ... | 1992 | 1359768 |
| isolation and purification of hsv-1 specific transfer factor produced by hsv-1 immunized goat leukocyte dialysate. | a herpes simplex virus type 1 (hsv-1)-specific transfer factor (tf), was separated and purified from the leukocyte dialysate of goats immunized with hsv-1 using affinity chromatography on antigen-sorbent and reversed phase high performance liquid chromatography (rp-hplc). the antigen-specific activities of the starting dialysate and the isolated tf component (s) were examined by 51cr-labelled leukocyte adherence inhibition (51cr lai) assay. the analytical hydrophobic interaction hplc (hi-hplc) a ... | 1992 | 1360750 |
| inhibition of hsv-1-specific cytotoxic t lymphocytes by recombinant-derived gp120 of hiv-1. | the ability of human immunodeficiency virus type-1 (hiv-1) and recombinant hiv-1 gp120 to prevent target cell lysis by herpes simplex virus type 1 (hsv-1)-specific cytotoxic t lymphocytes (ctl) was assessed by limiting dilution analysis. live and inactivated hiv-1 as well as recombinant-derived gp120 all substantially inhibited hsv-1-specific ctl. soluble cd4 antigen reversed the inhibition by gp120 when simultaneously added with gp120 to the assay. in addition, the monoclonal anti-cd4 antibody ... | 1992 | 1361737 |
| depletion of t-lymphocyte subsets in murine herpes-simplex-virus retinitis. | uniocular injection of herpes-simplex virus type 1 into the anterior chamber of balb/c mice induced contralateral retinitis with relative preservation of the ipsilateral retina. overall 95% of t-cell deficient nude mice developed ipsi- and contralateral retinitis, suggesting the importance of t-cells in this model. we then depleted lymphocyte subsets in susceptible balb/c and resistant cb-17 and c57bl/6j mice using anti-cd4 (helper/inducer cells) or anti-cd8 (suppressor/cytotoxic cells) monoclon ... | 1992 | 1362368 |
| the glycoprotein b gene and its syn3 locus of herpes simplex virus type 1 are involved in the synthesis of virus-associated growth factor (hsgf-1). | a putative growth factor (hsgf-1) associated with herpes simplex virus type 1 (hsv-1), which is similar to prgf associated with pseudorabies virus, and/or hsgf-2 associated with hsv-2, was described. experiments with four syncytial (syn) and four nonsyncytial (syn+) hsv-1 strains showed that the ability of this virus to produce hsgf-1 in infected cells is associated with the syn+ phenotype. double infection of cells with syn+ and syn strain resulted either in enhancement or complete inhibition o ... | 1992 | 1363988 |
| evidence for an altered kinetics of dna excision-repair in cells infected by herpes simplex virus type 1. | in cells infected with herpesviruses a series of host cell nuclear changes can be observed in a temporal sequence. such changes include chromosome aberrations. the precise mechanism by which virus infection produces chromosome damage is not known, but we have previously reported that herpes simplex virus type 1 (hsv-1) induces a significant number of single-stranded breaks in the host cell dna at early hours of infection and in a time-dependent fashion. here, it is reported that hsv-1-infected c ... | 1992 | 1364017 |
| varicella-zoster virus open reading frame 61 protein is functionally homologous to herpes simplex virus type 1 icp0. | the varicella-zoster virus (vzv) open reading frame 61 (orf61) protein is thought to be the homolog of herpes simplex virus type 1 (hsv-1) icp0, based on gene location and limited amino acid homology. however, hsv-1 icp0 trans activates hsv-1 genes, while vzv orf61 protein trans represses the function of vzv trans activators on vzv promoters in transient expression assays. to investigate the functional relatedness of hsv-1 icp0 and vzv orf61 protein, we established vero and mewo cell lines which ... | 1992 | 1366099 |
| domains of herpes simplex virus i glycoprotein b that function in virus penetration, cell-to-cell spread, and cell fusion. | herpes simplex virus 1 glycoprotein b (gb) is one of 10 glycoproteins in the virion envelope and in the membranes of infected cells. it is required for infection of cells in culture and functions in penetration of the cell by fusing the virion envelope with the plasma membrane. in studies to map the functional domains on hsv-1 gb, we reported that epitopes of potent neutralizing antibodies cluster in three major antigenic domains, d1, d2, and d5a. d1 contains continuous epitopes in the very amin ... | 1992 | 1370130 |
| activation of nk cells in mice following corneal infection with herpes simplex virus type-1. | natural killer (nk) cells are large granular lymphocytes that mediate antigen nonspecific, non-major histocompatibility complex (mhc) restricted lysis of virus infected cells. they are thought to play a role in innate resistance to herpes simplex virus (hsv) infections. in most animal studies reported to date, the virus was injected intraperitoneally, not a natural route of infection. using a murine model of acute hsv-1 ocular infection, we demonstrate that increased splenic nk activity is induc ... | 1992 | 1370438 |
| icp22 homolog of equine herpesvirus 1: expression from early and late promoters. | the complete nucleotide sequence of the short region, made up of a unique segment (us; 6.5 kb) bracketed by a pair of inverted repeat sequences (ir; 12.8 kb each), of the equine herpesvirus 1 (ehv-1) genome has been determined recently in our laboratory. analysis of the ir segment revealed a major open reading frame (orf) designated ir4. the ir4 orf exhibits significant homology to the immediate-early gene us1 (icp22) of herpes simplex virus type 1 and to the icp22 homologs of varicella-zoster v ... | 1992 | 1370553 |
| neutralizing epitopes on herpes simplex virus-1-expressed rotavirus vp7 are dependent on coexpression of other rotavirus proteins. | we constructed a recombinant thymidine kinase-negative herpes simplex virus type 1 (hsv-1) that expressed the rotavirus major outer capsid glycoprotein, vp7. in the recombinant hsv-1, a promoter from the 5' noncoding region of the hsv-1 glycoprotein b locus regulated the expression of vp7 as a hsv-1 gamma 1 gene product. hsv-1-expressed vp7 resembled rotavirus-expressed vp7 in its sds-page mobility, high mannose-type glycosylation, disulfide bonding, perinuclear to cytoplasmic localization, intr ... | 1992 | 1371025 |
| hexamethylene bisacetamide stimulates herpes simplex virus immediate early gene expression in the absence of trans-induction by vmw65. | hexamethylene bisacetamide (hmba) and dmso are known to induce differentiation of cultured erythroleukaemic cells and to enhance the reactivation of latent herpes simplex virus (hsv) after explantation of ganglia. we report that the presence of these compounds in cell culture medium overcomes the replication defect of in1814, an hsv-1 mutant with an insertion mutation that inactivates the virion trans-inducing factor, vmw65 (vp16). the effect of hmba was not cell type-specific and was attained e ... | 1992 | 1371540 |
| cytotoxicity of a replication-defective mutant of herpes simplex virus type 1. | replication-defective mutants of herpes simplex virus type 1 (hsv-1) may prove useful as vectors for gene transfer, particularly to nondividing cells. cgal delta 3 is an immediate-early gene 3 (ie 3) deletion mutant of hsv-1 that expresses the lacz gene of escherichia coli from the human cytomegalovirus immediate-early control region but does not express viral early or late genes. this vector was able to efficiently infect and express lacz in cells refractory to traditional methods of gene trans ... | 1992 | 1373198 |
| antigenic structure of the herpes simplex virus type 1 glycoprotein c: demonstration of a linear epitope situated in an environment of highly conformation-dependent epitopes. | a continuous epitope, situated within or in close proximity to antigenic site ii of the herpes simplex virus type 1-specified glycoprotein c (gc-1), was identified. the continuous linear nature of the epitope, defined by a monoclonal antibody c2h12, was established by three independent lines of evidence: (i) the epitope was detectable by immunoblot under denaturing and reducing conditions. (ii) the epitope was detectable by ripa of extracts from tm-treated hsv-infected cells, despite the malfold ... | 1992 | 1373286 |
| analysis of toxic and mutagenic activities of antiherpesvirus nucleosides against hela cells and herpes simplex virus type 1. | the toxic and mutagenic activities of five antiherpesvirus agents to hela cells and herpes simplex virus type 1 (hsv-1) were investigated. 5-iodo-2'-deoxyuridine (idu) and 9-beta-d-arabinofuranosyl-adenine (araa) showed very potent inhibitory effects on cell growth and the cloning efficiency of hela cells, whereas 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil (bv-arau), e-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) and 9-(2-hydroxyethoxymethyl)guanine (acv) showed less inhibitory effect. 50% in ... | 1992 | 1373847 |
| 2',5'-bis-o-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino-1'',2''- oxathiole-2'',2'-dioxide)pyrimidine (tsao) nucleoside analogues: highlyselective inhibitors of human immunodeficiency virus type 1 that are targeted at the viral reverse transcriptase. | a series of pyrimidine nucleoside analogues containing [2',5'-bis-o-(tert-butyldimethylsilyl)-3'-spiro-5''-(4''-amino- 1'',2''-oxathiole-2'',2''-dioxide)]-beta-d-ribofuranose as the pentose were found to inhibit human immunodeficiency virus type 1 [hiv-1(iiib)] replication at a concentration of 0.06-0.8 microm but were not cytotoxic at a 1000- to 10,000-fold higher concentration. these nucleoside derivatives were also effective against various other hiv-1 strains, including those resistant to 3' ... | 1992 | 1374900 |
| computer prediction of antigenic and topogenic domains in hsv-1 and hsv-2 glycoprotein b (gb). | the envelope glycoprotein b (gb) coded for by the herpes simplex virus type 1 (hsv-1) ul27 gene is similar to the amino acid (aa) sequence of the gb coded by a homologous gene in hsv-2 dna. the putative antigenic domains in hsv-1 and hsv-2 gb glycoproteins were analyzed on a comparative basis by suitable computer programs, which allowed the prediction of putative antigenic and topogenic domains. the computer-derived domains were compared to experimentally reported antigenic domains in hsv-1 gb g ... | 1992 | 1375407 |
| kinetics of inhibition of human immunodeficiency virus type 1 (hiv-1) reverse transcriptase by the novel hiv-1-specific nucleoside analogue [2',5'-bis-o-(tert-butyldimethylsilyl)-beta-d-ribofuranosyl]-3'-spiro-5 "- (4"-amino-1",2"-oxathiole-2",2"-dioxide)thymine (tsao-t). | [2',5'-bis-o-(tert-butyldimethylsilyl)-beta-d-ribofuranosyl]-3'-spiro- 5"-(4"-amino-1",2"-oxathiole-2", 2"-dioxide)thymine (tsao-t) is a representative of a novel class of nucleoside analogues that are endowed with a potent and specific activity against human immunodeficiency virus (hiv) type 1 and are targeted at the hiv-1 reverse transcriptase (rt). inhibition of hiv-1 rt by tsao-t was reversible and noncompetitive with respect to dgtp as the substrate and poly(c).oligo(dg) as the template/pri ... | 1992 | 1376314 |
| in vitro toxicological evaluation of isis 1082, a phosphorothioate oligonucleotide inhibitor of herpes simplex virus. | isis 1082, a phosphorothioate oligonucleotide targeted to a translation initiation codon of herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) virion capsid protein ul13 inhibits in vitro viral replication. to better understand the pharmacological properties of isis 1082, we examined its effects in nonvirally infected hela cells by using a number of cytotoxicity assays. our data indicate that isis 1082 had no effect on hela cell viability as measured by cellular prolifer ... | 1992 | 1377898 |
| genetic analysis of type-specific antigenic determinants of herpes simplex virus glycoprotein c. | herpes simplex virus type 1 (hsv-1) glycoprotein c (gc-1) elicits a largely serotype-specific immune response directed against previously described determinants designated antigenic sites i and ii. to more precisely define these two immunodominant antigenic regions of gc-1 and to determine whether the homologous hsv-2 glycoprotein (gc-2) has similarly situated antigenic determinants, viral recombinants containing gc chimeric genes which join site i and site ii of the two serotypes were construct ... | 1992 | 1378512 |
| epitopes of herpes simplex virus type 1 glycoprotein b that bind type-common neutralizing antibodies elicit type-specific antibody-dependent cellular cytotoxicity. | a panel of 45 well-characterized monoclonal antibodies (mabs) reactive to glycoprotein b (gb) of herpes simplex virus (hsv) type 1 was tested by elisa and in antiviral functional assays (that included virus neutralization, antibody-dependent cellular cytotoxicity (adcc), and antibody-dependent complement-mediated lysis), using type 1 or 2 virus strains. all mabs were elisa-reactive. eleven of the mabs mediated neutralization and 9 mediated adcc. all of the adcc epitopes were contained within the ... | 1992 | 1380051 |
| soluble glycoprotein d blocks herpes simplex virus type 1 infection of rat eyes. | herpes simplex virus type 1 (hsv-1) ocular infection in rats was blocked by treating the eyes with uv-inactivated virions containing glycoprotein d (gd) prior to ocular challenge. in contrast, rats treated with uv-inactivated virions lacking gd were not protected. a soluble, truncated form of hsv-2 gd (gd-2t) also protected against ocular infection. treatment with gd-2t not only reduced mortality but also restricted progression of pathology and reduced the amount of viral antigen in the cornea. ... | 1992 | 1380093 |
| the proportion of galanin-immunoreactive neurons in mouse trigeminal ganglia is transiently increased following corneal inoculation of herpes simplex virus type-1. | to investigate whether neurobiological functions are modified by viral infection, we inoculated mouse corneas with herpes simplex virus type 1 (hsv-1) and examined neuronal galanin content in trigeminal ganglia at selected survival times. hsv-1 antigen appeared in neurons at day 3, peaked at day 6 and disappeared by day 11. increased galanin positivity was first seen at day 6, peaked at day 10 and approached control values by day 21. this result provides further evidence that the biological prog ... | 1992 | 1380144 |
| differential mapping of fc gamma-binding and monoclonal antibody-reactive epitopes on ge, the fc gamma-binding glycoprotein of herpes simplex virus type 1. | the entire 396 residue extracellular sequence of ge the hsv-1 fc gamma-binding glycoprotein has been studied to determine epitopes binding to two mab ii-481 and 88s previously demonstrated to react with ge at or near the fc gamma-binding regions. overlapping 7-mers constructed from the established sequence were tested with mab ii-481 and 88s along with their fab fragments. control mab of the same igg 2b subclass as well as whole rabbit and human igg and fc were also tested for binding to overlap ... | 1992 | 1382102 |
| [development of resistance of herpes simplex virus to antivirus drugs in vitro]. | resistant variants of hsv-1 wt strain to acv, cc, dhpg and pfa can be selected after several passages in drug-containing cultures. the degree of resistance of different variants varied and emerged at different times. the combination of antiviral agents can delay and even prevent the emergence of drug-resistant variants. at the same time, the concentration, dose and, therefore, toxicity of the antiviral agents can also be reduced. so, it is a feasible method for treating resistant hsv infections. ... | 1992 | 1382356 |
| glycoprotein gp116 of human cytomegalovirus contains epitopes for strain-common and strain-specific antibodies. | glycoprotein gp116 of human cytomegalovirus (hcmv) is a target for neutralizing antibodies. gp116 is a component of the gci complex which consists of gp58 and gp116. like its homologue, glycoprotein b of herpes simplex virus type 1, gp116 contains a highly antigenic region in the n-terminal part of the molecule, between amino acids 28 and 84. prokaryotic expression plasmids and synthetic peptides were used to define binding sites for mouse and human monoclonal antibodies (mabs) as well as hcmv c ... | 1992 | 1383409 |
| methylation of hsv-1 dna as a mechanism of viral inhibition: studies of an analogue of methyldeoxycytidine: trifluoromethyldeoxycytidine (f3mdcyd). | although several hypomethylating agents such as 5-azadeoxycytidine and 5-fluorodeoxycytidine have been shown to activate transcription after incorporation into viral or cellular dna, agents which selectively affect the methylation status of virus-infected cells have not been described. studies on the antiviral effect of the methyldeoxycytidine (mdcyd) analogue trifluoromethyldeoxycytidine (f3mdcyd) showed significant antiviral activity against herpes simplex virus type 1 (hsv-1). this analogue o ... | 1992 | 1384426 |
| defective b-cell and regulatory t-cell function in wiskott-aldrich syndrome. | we report two chinese boys with wiskott-aldrich syndrome presenting with gastro-intestinal bleeding, eczema and recurrent infection. they had thrombocytopenia and the mean platelet volume was small. serum igg and iga were elevated and lymphocyte proliferation in response to phytohaemagglutinin, concanavalin a and pokeweed mitogen was defective. despite documented herpes simplex virus type 1 and cytomegalovirus infection in one patient, he did not mount any humoral response. the generation of ant ... | 1992 | 1396930 |
| diagnosis and management of orofacial herpes simplex virus infections. | hsv disease remains a significant problem for the dental profession because of its widespread occurrence and ready transmission. fortunately, available diagnostic techniques are highly sensitive and specific, antiviral therapy is effective in limiting the course of infection, and contemporary infection control procedures adequately safeguard dental personnel and the patient. unfortunately, there is still a large segment of the population already infected with hsv-1 and the number of immunosuppre ... | 1992 | 1397439 |
| baculovirus expressed herpes simplex virus type 1 glycoprotein c protects mice from lethal hsv-1 infection. | a recombinant baculovirus (vac-gc1) was constructed that expresses the glycoprotein c (gc) gene of herpes simplex virus type 1 (hsv-1). when sf9 cells were infected with this recombinant, a protein that was smaller in size than authentic hsv-1 gc was detected by western blotting using anti-gc polyclonal antibody. the recombinant gc was susceptible to tunicamycin, partially resistant to endo-h, and was found on the membrane of sf9 cells. antibodies raised in mice to recombinant gc reacted with gc ... | 1992 | 1416910 |
| decrease in mesencephalic dopamine autoreceptors in experimental herpes simplex encephalitis. | brain dopamine receptors were determined in experimental herpes encephalitis using an animal model, where herpes simplex virus type 1 was inoculated onto the cornea of rabbits. the animals exhibit an asymmetric posture and circling to the side of inoculation, which appears to be connected to the altered dopamine transmission in the mesostriatal system. in this study striatal and mesencephalic d-1 and d-2 dopamine receptors were measured by radioligand techniques using 3h-sch 23390 and 3h-spirope ... | 1992 | 1418868 |
| assessment of the anti-viral effect of a short-term oral treatment of mice with live saccharomyces cerevisiae cells. | for assessing the efficacy of antiviral treatments, influenza and herpes virus hsv-1 infections of varying degrees of severity have been produced. the infections proved to be reproducible with respect to both their course and death rate. these infections also exhibited a course slow enough to permit the assessment of treatments under conditions mimicking human infections and lent themselves to the choice of the best adapted strategy to treat an infection. a short-term oral treatment with live ce ... | 1992 | 1426651 |
| standardized mouse infection models as a way of evaluating the potency of anti-infectious agents. | standardized bacterial and viral mouse infection models have been developed. infections with extracellular bacteria (k. pneumoniae, s. pneumoniae, s. pyogenes a) were produced by either of two routes: via the intravenous route (i.v.) resulting in septicaemia and the intranasal route (i.n.) giving infections confined to the respiratory apparatus. infections with intracellular bacteria (l. monocytogenes, s. typhimurium) were produced only by the i.v. route. two types of viral infection, mild and s ... | 1992 | 1426654 |
| hailey-hailey disease--exacerbation by herpes simplex virus and patch tests. | benign familial chronic pemphigus, or hailey-hailey disease, is a rare hereditary condition characterized by development of blisters at sites of friction such as the neck, axillae and groin. contact sensitivity to topical medications is reported to be common and routine patch testing has been strongly advocated. we report a case of hailey-hailey disease in a 43-year-old veterinary surgeon who presented with an acute exacerbation of his disease caused by herpes simplex virus type 1 (hsv). patch t ... | 1992 | 1451302 |
| studies of antibody to herpes simplex virus fc gamma-binding protein ge in patients with rheumatoid arthritis, juvenile rheumatoid arthritis and normal controls. | igg antibody to ge, the fc gamma-binding herpes simplex 1 (hsv-1) viral glycoprotein, was studied in 49 rheumatoid arthritis (ra) patients and 43 normal controls. antibody to gd, another important hsv-1 antigen, was assayed in parallel. no difference between ra patients and normal controls was found in levels of anti-ge antibody measured by reactivity of igg f(ab')2 fragments reacting with ge coated to elisa plates. no difference in anti-gd antibody was recorded between normals and patients with ... | 1992 | 1462119 |
| antiviral effects of recombinant human tumor necrosis factor-alpha in combination with natural interferon-beta in mice infected with herpes simplex virus type 1. | the protective effects of combination therapy utilizing recombinant human tnf-alpha (rtnf-alpha) and natural murine interferon-beta (ifn-beta) in mice infected with herpes simplex virus type 1 (hsv-1) was investigated. mice treated with rtnf-alpha alone at all of the doses tested (a single i.v. administration, 2.3-2,300 micrograms/kg; multiple i.p. administrations 0.4-250 micrograms/kg) as well as mice that received ifn-beta alone at doses of 16 x 10(4) u/kg or less resulted in a 0% survival rat ... | 1992 | 1463323 |
| in vitro virucidal effects of allium sativum (garlic) extract and compounds. | garlic (allium sativum) has been shown to have antiviral activity, but the compounds responsible have not been identified. using direct pre-infection incubation assays, we determined the in vitro virucidal effects of fresh garlic extract, its polar fraction, and the following garlic associated compounds: diallyl thiosulfinate (allicin), allyl methyl thiosulfinate, methyl allyl thiosulfinate, ajoene, alliin, deoxyalliin, diallyl disulfide, and diallyl trisulfide. activity was determined against s ... | 1992 | 1470664 |
| evaluation of new anti-infective drugs for the treatment of genital infections due to herpes simplex virus. infectious diseases society of america and the food and drug administration. | this guideline addresses clinical trials of new therapies for genital infections due to herpes simplex virus (hsv). of the two types of virus, hsv-2 is the more common pathogen. both hsv-1 and hsv-2 become latent in sacral nerve root ganglia and intermittently reactivate. patients who have frequent recurrences (more than four per year) may be candidates for long-term suppressive therapy. in both first-episode and recurrent genital hsv infections, lesions should be cultured for hsv. testing for h ... | 1992 | 1477256 |
| synthesis and biological activity of new heterocyclic structures: [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c] [1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo [4', 5': 4,5] pyrimido[6,1-b][1,3]thiazine. | some derivatives of thiazolo[3,2-c]pyrimidine, pyrimido[6,1-b][1,3]thiazine, thiazolo[2,3-i]purine, [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c][1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo[4',5':4,5]pyrimido[6,1-b][1,3]thiazine were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely, escherichia coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, streptococcus faecalis, bacillus sub ... | 1992 | 1482518 |
| mutagenesis by apurinic sites in normal and ataxia telangiectasia human lymphoblastoid cells. | we used a shuttle vector based on the epstein-barr virus origin of plasmid replication (orip) to determine the types of mutations induced by depurination in human cells. plasmid dna was incubated at ph 2 at 40 degrees c for various times to induce up to 20 apurinic (ap) sites per 9.7-kb plasmid and electroporated into lymphoblastoid cells derived from either a normal individual or an ataxia telangiectasia patient. after replication of the vector in the human cells, plasmid dna was isolated and a ... | 1992 | 1503643 |
| herpes gladiatorum: a growing problem in sports medicine. | herpes skin infections among wrestlers--called herpes gladiatorum--occur frequently among high school and college wrestlers nationwide. contact with opponents with active herpes infections appears to be the strongest risk factor associated with acquisition of infection. previously acquired antibody to herpes simplex virus 1 infection may offer some protection from acquiring this disease. morbidity associated with this disease can be significant, and widespread efforts to educate participants in ... | 1992 | 1511621 |
| effect of herpes simplex virus type 1 infection on cytokine gene expression in activated murine peritoneal macrophages. | the intrinsic resistance to herpes simplex virus type 1 (hsv-1) of murine peritoneal macrophages (pm phi) obtained after in vivo infection of different stimuli has been investigated and shown to vary depending on the state of m phi activation. activation of m phi by c. parvum (cp-m phi) or by an avirulent strain of s. typhimurium (sal-m phi) increased the permissiveness of m phi to hsv-1 infection as evidenced by increased hsv-1 immediate early (ie) gene expression, synthesis of ie proteins, and ... | 1992 | 1514441 |
| [markers of hbv infection in drug addicts]. | prevalence of markers of hbv infection was tested in two cohorts of drug addicts: in 140 addicts whose sera were drawn in the years 1985-1986 and in 100 addicts whose sera were drawn in the years 1988-1989. hbsag was found in 10 (7%) patients in the earlier cohort and in 12 (12%) addicts in the latter, while at least one marker of hbv infection was present in 39% and 69%, respectively no correlation was found between the presence of markers of hbv infection and age, gender, or hiv status, howeve ... | 1992 | 1523136 |
| transmission of herpes simplex virus type 1 infection in an intensive care unit. | | 1992 | 1524332 |