| the differential binding of antibody from the sera of patients with pemphigus and pemphigoid to isolated guinea-pig epidermal cells. | a method for preparing isolated epidermal cells using trypsin is described. the isolated cells retain the ability to bind antibody from patients with pemphigus or with pemphigoid in a disease-specific. | 1975 | 53062 |
| secretory component of the guinea-pig. | free secretory component (fsc) was purified from guinea-pig milk by gel-filtration and immunoabsorption on anti-sc antibodies. guinea-pig fsc cross-reacted with antisera to human fsc, and the reverse. guinea-pig and human fsc resembled each other in molecular size, electrophoretic mobility and heterogeneity, as well as by the existence of antigenic determinants restricted to the unassociated form of the molecule. guinea-pig fsc associates in vitro with guinea-pig igm. distribution of disulphide ... | 1975 | 53196 |
| hapten and carrier specificities of cellular and humoral responses to highly substituted dinitrophenyl-human gamma-globulins in guinea-pigs. | delayed hypersensitivity (dh) and antibody reactions to the carrier and to the hapten have been studied in guinea-pigs immunized with different doses of highly substituted dinitrophenyl-human gamma-globulins (dnp56-hgg) in freund's complete adjuvant (fca). the results confirm that dh reactions are specific for the carrier while antibody-mediated reactions are specific for the hapten in the early stages of the immune response. later in the response, however, dh reactions to the hapten as well as ... | 1975 | 53199 |
| stimulation and inhibition of anti-hapten responses in guinea pigs immunized with hybrid liposomes. | guinea pigs were immunized with liposomal model membranes containing phosphatidylethanolamine (pe) or glycerophosphorylethanolamine (gpe) derivatives in which the amino function was substituted with either dinitrophenylaminocaproyl (dnp-cap) or mono(p-azobenzenearsonic acid)tyrosyl (aba-tyr) residues. previous studies have demonstrated that hapten-specific antibodies are elicited by dnp-cap-pe or aba-tyr-pe sensitized liposomes and that cell-mediated immunity is induced by aba-tyr-pe (but not dn ... | 1975 | 53840 |
| the solid phase radioimmunoassay technique for hepatitis b (surface) antigen: effects of progressive modification. | serum specimens submitted for confirmation of hepatitis b antigen (hbsag) reactivity were tested by counterelectrophoresis (cep) and by three modifications of the solid phase radioimmunoassay "sandwich" technique (spria). in addition, the specificity of each reaction was determined for two of the spria test methods. one hundred seventy-five of the 688 specimens were reactive by all techniques and a further 60 ere specifically reactive by two of modifications of spria. an additional five specimen ... | 1975 | 53923 |
| ascorbic acid requirements and metabolism in relation to organochlorine pesticides. | those organochlorine pesticides which possess both high lipoid solubility and high resistance to biodegradation are prone to accumulation in animal tissues and produce relatively long-term effects as toxicants. such compounds, typified by ddt, dieldrin, and lindane, are profound inducers of hepatic microsomal enzymes, including parts of the glucuronic acid and ascorbic acid biosynthetic pathways. consequently, administering such pesticides to rats in accompanied by enhanced formation and excreti ... | 1975 | 54013 |
| immunological studies on a yeast peptido-galactomannan. nature of antigenic determinants reacting with rabbit antisera and those involved delayed hypersensitivity in guinea pigs. | | 1975 | 54049 |
| proceedings: incomplete inhibition of sodium transport-related aerobic metabolism upon exposure of guinea pig renal cortex slices to ouabain. | | 1975 | 54075 |
| proceedings: [influence of na and k on inhibition of (na + k)-atpase of guinea pig heart by digoxin or calcium]. | | 1975 | 54140 |
| proceedings: the effects of hypoxia on the membrane resistance of ventricular muscle fibres of the guinea pig. | | 1975 | 54144 |
| recording site in organ of corti determined by electrode marking technique. | microelectrodes containing 9% niagara sky blue dye were used to record and electrophoretically mark cells in the guinea pig organ of corti. the tissue was fixed in acidified glutaraldehyde and the marked cells were located using the surface preparation technique. | 1976 | 54159 |
| studies on the inhibition of macrophage migration induced by soluble antigen-antibody complexes. | mixtures of serum of freund's complete adjuvant (fca) immunized guinea-pigs and tuberculin ppd consistently inhibited the in vitro migration of peritoneal exudate cells (pec) of normal guinea-pigs. it is shown that this inhibitory effect is due to a soluble complex between an igg2 antibody and ppd. by separation of ppd on sephadex g-200 two peaks were obtained, corresponding respectively to molecular weights of at least 800,000 and 25,000, separated by a plateau. material derived from both peaks ... | 1975 | 54230 |
| identification of c-cells in normal and goitrous rat thyroid tissues using antiserum to rat thyrocalcitonin and the immunoperoxidase bridge technique. | application of the immunoperoxidase bridge technique to the light microscopic localization of c-cells in rat thyroid tissue is described. guinea pig antisera to rat thyrocalcitonin (tct) were produced by the injection of highly purified rat tct (100-300 mrc u/mg) emulsified in complete freund's adjuvant. a 1:1000 dilution of the antiserum used in this study gave a strong positive reaction with rat c-cells, and 1 ml of undiluted antiserum provided sufficient material for staining approximately 50 ... | 1975 | 54255 |
| species specificity of thromboplastin. a phylogenetic study. | having studied the influence of thromboplastin preparations derived from cold and warm-blooded species on the plasma of 12 vertebrates (carp, frog, turtle, hen, rabbit, rat, mouse, guinea-pig, ground squirrel, dog, sheep and man) we have established that among the species far from each other phylogenetically, the phenomenon of species specificity could be demonstrated in general. it was found that the extrinsic coagulation system measured by quick times can well be activated in every examined sp ... | 1975 | 54309 |
| antigenic properties of polymers formed by beta-lactam antibiotics. | purified polymers have been isolated from 6-aminopenicillanic acid and from semi-synthetic penicillins and cephalosporins. all the polymers were shown to react with rabbit antibody of penicillins and cephalosporins. all the polymers were shown to react with rabbit antibody of penicilloyl specificity, as demonstrated by passive cutaneous anaphylaxis in guinea pigs, and the reactions were shown to be penicilloyl-specific by hapten inhibition experiments. the cephalosporin-derived polymers in addit ... | 1976 | 54343 |
| suppression and reversal of allergic encephalomyelitis in guinea pigs with a non-encephalitogenic analogue of the tryptophan region of the myelin basic protein. | the administration of synthetic peptide s42 leads to suppression and reversal of experimental allergic encephalomyelitis (eae) induced in guinea pigs by myelin basic protein. peptide s42 contains a linear sequence of 21 amino acid residues, h-phe-ser-trp-gln-lys-phe-ser-trp-gln-lys-phe-ser-trp-gln-lys-phe-ser-trp-gln-lys-gly-oh, made up of four repeating unit sequences of h-phe-ser-trp-gln-lys-oh in addition to a c-terminal glycine. injected at relatively high doses, peptide s42 is non-encephali ... | 1976 | 54384 |
| characteristics of the major internal protein and rna-dependent dna polymerase of bovine leukaemia virus. | a virus designated bovine leukaemia virus (blv), associated with leukaemia in cattle and previously demonstrated to induce the disease in sheep, was purified from chronically infected sheep cell cultures. electrophoretic analysis showed a major protein of mol. wt. about 24,000 (p24) which reacted in gel diffusion and complement-fixation tests with sera from naturally infected cattle, experimentally infected sheep, and guinea pigs immunized with p24. blv p24 has an isoelectric point of 8-6. inter ... | 1975 | 54405 |
| biochemical effects of sensibilization of guinea pigs with basic encephalitogenic protein. | | 1975 | 54889 |
| radioimmunoassay of bovine heart protein kinase. | immunization of guinea pigs with bovine cardiac camp-dependent protein kinase (atp:protein phosphotransferase, ec 2.7.1.37) resulted in the development of precipitating antibodies to the camp-binding subunit of the enzyme. both the phosphorylated and nonphosphorylated camp-binding protein of the protein kinase reacted with the antiserum. a radioimmunoassay was developed that detects 10 ng of holoenzyme and permits measurement of enzyme concentrations in bovine cardiac muscle. bovine liver, kidne ... | 1976 | 54918 |
| [study of the role of type-specific antigen in the process of shigella flexneri interaction with epithelial cell and macrophage cultures]. | epithelial cells of the hep-2 line infected in parallel by genetically connected strains of sh. flexneri, differing in the capacity to synthesize the type antigen were studied morphologically. the type antigen proved to show no significant influence on the penetration of dysentery bacilli into the cell. a study of the process of phagocytosis of the mentioned strains by a culture of peritoneal macrophages of guinea pig demonstrated that the presence of a type antigen communicated to the bacteria ... | 1975 | 55022 |
| attempts to induce sensitization in guinea pigs with nickel complexes. | a technique for the induction of sensitization in pigs with a nickel-alanine conjugate has been reported by other investigators. similar results were observed in our experiments in mini-pigs. however, using the identical technique, we were unable to induce sensitization in guinea pigs with niso4, with nickel-alanine or other nickel-amino acid complexes, or with a complex of nickel with soluble guinea pig skin extract. these results indicate that nickel-amino acid complexes and nickel-guinea pig ... | 1975 | 55041 |
| progesterone-binding globulin and testosterone-binding activity in guinea pig serum during pregnancy: relationship to progesterone and oestrogens. | progesterone levels in serum have been determined throughout pregnancy in guinea-pigs by a competitive protein-binding technique, using pregnant guinea pig plasma protein as binding agent. the concentrations of this protein (progesterone-binding globulin, pbg) as well as the testosterone-binding activity (tba) have been quantitated by means of equilibrium dialysis. in order to correlate these parameters to the endogenous oestrogen production, the urinary oestrogen excretion was recorded. the con ... | 1976 | 55049 |
| immunologic release of histamine from bovine polymorphonuclear leukocytes. | blood from calves sensitized to horse serum was fractionated to yield a suspension containing 94% granulocytes. these cells released up to 90% of their total histamine when exposed to the antigen. this is a noncytotoxic reaction similar to that observed in human beings, rabbits, and guinea pigs. higher concentrations of antigen caused inhibition of histamine release. this immunologic reaction may be important in bovine hypersensitivity, especially in the lungs, where intravascular aggregations o ... | 1975 | 55087 |
| vestibular end organ mapping in the squirrel monkey. | vestibular end organ mapping (surface preparation) was performed in squirrel monkeys by using succinic dehydrogenase staining. comparing sensory hair cell counts and surface area measurements of the squirrel monkey to those of man and the guinea pig, the values obtained from the squirrel monkey were between those of man and the guinea pig. when regional (central versus peripheral) cell density was compared, the density of the peripheral area was 1.62x more on the average than that of the central ... | 1975 | 55117 |
| experimental allergic encephalomyelitis-inducing activity of synthetic polyadenylic and polyuridylic homopolymers and complexes in guinea pigs. | | 1976 | 55310 |
| species-specific tissue antigens. iii. immunological relationships of enzymic antigens in various species. | immunological relationships have been investigated with acid and alkaline phosphatases, cystine aminopeptidase, beta-acetylglucosaminidase, beta-glucuronidase, catalase and l-glutamate dehydrogenase of human, monkey, mouse, rat, rabbit, dog, cattle, sheep, cat, pig, guinea-pig and chicken organ extracts by means of immunodiffusion and immunoelectrophoresis. extensive cross-reactions among the antigens of most of the enzymes were observed. however, enzymic proteins of acid and alkaline phosphatas ... | 1975 | 55325 |
| inhibition of anaphylactic histamine release by forssman antiserum. i. characteristics of the reaction and inhibitor. | forssman antiserum produced in rabbits immunized with sheep erythrocyte stromata was found to contain an igg antibody which inhibited both passive anaphylactic sensitization of guinea-pig lung and also histamine-releasing activity of soluble immune complexes. this forssman antibody did not itself cause histamine release or depletion of lung histamine stores. the igm haemolysin component of the forssman antiserum was not associated with inhibitory activity. the inhibition by the igg forssman ant ... | 1976 | 55380 |
| studies in experimental eosinophilia. xi. dependence of eosinophilia, apparently induced by histamine, on acidity. | a solution of histamine in saline elicited eosinophil response in both lymph nodes and the blood stream. the lymph node response was not inhibited by the antihistamine, mepyramine maleate, but was inhibited by puromycin. both the lymph node and the systemic response were essentially abolished when the ph of the solution (5.0 and 4.7, respectively) was brought to neutrality by phosphate buffer. serum could bring the ph of histamine to neutrality or higher, but the histamine-serum mixture retained ... | 1976 | 55395 |
| the effect of thiols on the immunologic release of slow reacting substance of anaphylaxis. ii. other in vitro and in vivo models. | in the presence of l-cysteine, a selective and marked enhancement of the in vitro, immunologic release of slow reacting substance of anaphylaxis (srs-a) from human peripheral leukocytes, sensitized monkey lung fragments, and sensitized guinea pig lung fragments was observed. in the rat, cysteine, but not sodium sulfide, enhanced the calcium ionophore (a23187)- induced release of srs-a in vitro from mixed rat peritoneal cells and in vivo from the rat peritoneal cavity. pretreatment of rats with c ... | 1976 | 55439 |
| parital characterization of the rat anti-encephalitogenic protein (rscp). | a protein antigenically similar to the anti-encephalitogenic bovine spinal cord protein (bscp) was detected in saline extracts of rat nervous tissues by immunodiffusion analyses using a rabbit anti-bscp serum. rat scp (rscp) appears to be evenly distributed throughout all parts of the rat nervous system and occurs also in the thymus, thyroid, and adrenal glands. although immunodiffusion analyses indicated that rscp shares some antigenic sites with bscp, anti-rscp sera reacted only with rscp, ind ... | 1976 | 55440 |
| delayed hypersensitivity and granulomatous response after immunization with protein antigens associated with a mycobacterial glycolipid and oil droplets. | a myocardial glycolipid (p3) mixed with protein antigens in oil-in-water emulsion induced lasting delayed hypersensitivity (dh) and granulomatous inflammation after intradermal injection into guinea pigs. this did not occur when p3 and bovine serum albumin (bsa) were given in freund's incomplete adjuvant. the oil-in-water emulsions consisted of microscopic oil droplets suspended in aqueous medium. by separating oil and aqueous phases from bsa + p3 emulsion it was shown that antigen retained with ... | 1976 | 55442 |
| analysis of cell-surface markers with staphylococcal protein a: fluorescence studies on mammalian lymphoid determinants. | membrane antigens including different classes of immunoglobulins, transplantation antigens, beta2-microglobulin, t lymphocyte specific antigens, and virally determined surface components were investigated using fluorescein-labeled staphylococcal protein a in combination with cytofluorometric studies. lymphocytes of seven species: mouse, rat, guinea pig, pig, cow, monkey, and human, and of ten human lymphoma-derived lines were tested. analysis of the differential expression of surface markers rev ... | 1976 | 55446 |
| structural characteristics of the alloantigens determined by the major histocompatibility complex of the guinea pig. | the gpla b and ia (i region-associated) antigens are the products of genes found in the guinea pig major histocompatibility complex. because of their importance in immune response phenomena, a structural study of these antigens was undertaken. [3h]leucine and [3h]fucose were internally incorporated into guinea pig lymph node cells. the gpla b and ia antigens were solubilized by the nonionic detergent nonidet p-40, purified by affinity chromatography using an adsorbent column of lentil lectin, is ... | 1976 | 55459 |
| essential fatty acids and fetal brain growth. | the fetal brain accumulates long-chain (c20 and 22) polyunsaturated fatty acids--arachidonic and docosahexaenoic--during cell division. de-novo synthesis of these acids does not occur and they are thought to be either directly derived from food or by metabolism from linoleic and linolenic acids, respectively. administration of isotopically labelled linoleic and linolenic acids to pregnant guineapigs showed that only a small proportion of the label was converted to their respective long-chain pol ... | 1976 | 55720 |
| experimental autoimmune uveo-retinitis and specificity of retinal antigens. | chorio-retinal lesions induced in guinea pigs after one inoculation of bovine rod outer segments (ros) with complete freund's adjuvant are described with light and electron microscopy. the auto-antigenic activity of different preparations from bovine retina and uvea is compared for their efficacy to induce the disease. ros are much more active than total retina homogenate. pigment epithelium is active, and the effect of choroid is impaired after removal of pigment epithelium from the surface of ... | 1976 | 55959 |
| in vitro target cell lysis mediated by normal human lymphocytes (k cells) or monocytes. | guinea pig igg1 or igg2 anti-dinitrophenyl (dnp) antibody preparations were used for induction of target cell lysis by normal human lymphocytes (k cells) or monocytes. target cells were dnp-coated 51cr-labeled chicken erythrocytes. antibody concentrations were assayed by an ammonium sulphate precipitation technique. when antiserum was obtained by immunization with the antigen in freund's complete adjuvant (fca), igg2 antibodies predominated and were significantly more efficient inducers of k-cel ... | 1975 | 56029 |
| haemostatic plugs as a histological vital reaction in the skin wounds of guinea pigs. | applying a histological staining method (carstairs' modification of picro-mallory staining), haemostatic plugs could be demonstrated at points of vascular lesion in one-minute vital, or older, skin wounds of the guinea-pig on the average in 33% if the specimen was taken immediately after the reaction period, and in about 10% of wounds examined if the specimen was taken five days after death. no haemostatic plugs could be observed in postmortally inflicted skin wounds. | 1975 | 56105 |
| recurrent immunogenic interstitial keratitis. production in the guinea pig by extraocular means. | combinations of intracutaneous (ic) and intravenous (iv) injections of bovine gamma-globulin (bgg) or bovine serum albumin (bsa) induced interstitial keratitis in 24 of 29 guinea pigs. for ic injection, antigen was incorporated in complete freund adjuvant. when ic and iv bgg reactants were administered simultaneously, corneal opacities appeared after 11 to 19 days and persisted for periods of 2 to 11 days. following a free interval, a secondary reaction of somewhat shorter duration occurred. a b ... | 1976 | 56164 |
| antigenic determinants of hen egg-white lysozyme in delayed hypersensitivity. i. macrophage migration inhibition activities of lysozyme fragments. | five kinds of fragments of hen egg-white lysozyme (hl) were tested by macrophage migration inhibition (mmi) assay using peritoneal exudate cells (pec) of guinea pigs immunized with hl in complete freund's adjuvant. (see article). these four kinds of hl fragments were also shown to be composed of the immunodominant groups of the hl molecule for circulating antibody against hl in guinea pigs. the relationships between the antigenic sites related to circulating antibody and the cellular recognition ... | 1975 | 56171 |
| antigenic determinants of hen egg-white lysozyme in delayed hypersensitivity. ii. antigenicity and immunogenicity of the n- and c-terminal peptide. | various small fragments of (see article) which is one of the immunodominant groups of hen egg-white lysozyme (hl), were tested for macrophage migration inhibition (mmi) of peritoneal exudate cells (pec) from guinea pigs immunized with hl. p17 was split in the middle with cyanogen bromide. the terminal portion of (see article) showed positive mmi, whereas the non-terminal half of p17, p17i (sequence 13-27) only showed very weak mmi activity. a fragment derived from the middle portion of p17, p17m ... | 1975 | 56172 |
| proceedings: strain differences in the immune response to the encephalytogenic determinant in inbred guinea pigs. | | 1975 | 56327 |
| idiotypic analysis of lymphocytes in vitro. ii. genetic control of t-helper cell responsiveness to anti-idiotypic antibody. | when the igg1 fraction of anti-idiotypic antibodies raised in guinea pigs is injected into mice, sensitization of idiotypic t and b lymphocytes occurs (1-3). in the present study we analyze the genetic requirements for t-helper cell sensitization by anti-idiotypic antibody. this was done by measuring, in a suitable panel of mouse strains, helper cell responsiveness to two anti-idiotypic reagents which recognize distinct, strain-specific idiotypes, namely the a5a and the s117 marker. whenever hel ... | 1976 | 56419 |
| immune response of guinea-pigs to chemically abbreviated prepatent metastrongylus apri infection. | the effect of chemical abbreviation of the primary infection dose (pid) of 160 infective larvae of metastrongylus apri on the immune status of the guinea-pig host was studied. the criteria used for assessing the status of immunity consisted of clinical manifestations following administration of a challenge infection dose (cid) of 800 infective larvae of m. apri, the rate of worm recovery 15 days post-cid and the rate of mortality following administration of cid. among the guinea-pigs of the main ... | 1976 | 56737 |
| studies on guinea pig skin cell cultures. v. co-culture of pigmented melanocytes and albino keratinocytes, a model for the study of pigment transfer. | mixed cultures of melanocytes (m) and keratinocytes (k) are easily obtained from pigmented guinea pig ear skin. they are suitable for the study of pigment transfer from m to k. however, quantitation is difficult because many k are already loaded with pigment prior to cultivation. a technique is presented in which pigment-producing m are co-cultured with k of albino origin. pigmented guinea pig ear skin is split with trypsin and basal cells including m are harvested. the cell suspension is treate ... | 1976 | 56849 |
| the activation of antigens of the basement membrane zone by proteolytic enzymes in vitro. | following brief exposure of cryostat sections of human skin to the action of proteolytic enzymes (papain or trypsin), there was a pronounced increase in the antibody binding sites of the basement membrane zone, shown in indirect immunofluorescence by an increased intensity in fluorescence of the basement membrane zone as compared with preparations which had not previously undergone incubation, and by an increase of 5-6 (papain) or 4-5 (trypsin) titre dilution steps. this effect was practically a ... | 1976 | 56855 |
| kurloff cells. 1. histochemical characteristics of the kurloff bodies. | kurloff cells, characterized by a large inclusion body in ill-defined lymphoreticular cells, were induced in male guinea pigs by oestrogen administration. the inclusion body material was characterized by way of light- and electron microscopy and histochemical staining reactions. nucleo-protein were not detected, but certian tests for amino acids were observed and, at the peripheral parts of the inclusion bodies, phospholipid tests were positive. however, forced proteolysis of lipid extractions f ... | 1976 | 56867 |
| kurloff cells. 2. histochemical and morphological characteristics of the kurloff cells. | kurloff cells (kb cells) containing a glycoprotein inclusion body appear in femal guinea pigs and can be induced in males by oestrogen administration. these cells are found in bone marrow, thymus, and in the red pulp of the spleen, but not in the lymph nodes. gradually growing inclusion bodies were observed in a series of cells in these organs. morphological and histochemical features suggested an active synthesis of the inclusion body material: cisternae of the endoplasmic reticulum fused with ... | 1976 | 56868 |
| galactose oxidase as a tool to label a glycoprotein and to study its fixation on isolated guinea-pig ileum receptors. | | 1975 | 56877 |
| production of antisera with specificity for malignant melanoma and human fetal skin. | complement-dependent cytotoxic antibodies to common cell surface antigens of cultured melanoma cells were produced in guinea pigs. at appropriate dilution, melanoma antisera were cytotoxic only to melanoma target cells. following absorption with pooled lymphoid cells, additional absorption with melanoma cells but not absorption with fibroblasts or carcinoma cells was found to remove all cytotoxic activity from melanoma antisera. absorption with human fetal skin cells but not with autologous feta ... | 1976 | 56991 |
| leukocyte-migration inhibition in guinea pigs. i. correlation with skin test reactivity and macrophage-migration inhibition. | | 1976 | 57005 |
| beta2-microglobulins: isolation, properties, and distribution. | beta2-microglobulin is structurally related to immunoglobulin domains and is identical to the light chain of histocompatibility (hl-a) antigens. similar to free light chains of immunoglobulins, beta2-microglobulin is most easily isolated from urine. we have previously purified human beta2-microglobulin from urine of patients with renal tubular resorption defects. corresponding proteins have now been obtained from urine of rabbits and guinea pigs treated with sodium chromate. sequence studies hav ... | 1976 | 57069 |
| incompatibility between c3b and b of guinea-pig and man and its influence on the titration of the alternative pathway factors d and b in these two species. | the incubation of the proteins c3b, b, d and c3 induces enzymatic activation of b and of c3. this system of purified components was applied to the titration of the guinea-pig and human factors d and b in various homologous and heterologous reaction mixtures. on the basis of functional as well as of immunological tests, the d enzymes of both species were found to be related proteins. first, titrations of factor b revealed that both d enzymes activate homologous and heterologous b with comparable ... | 1976 | 57093 |
| inhibition of antigen-induced histamine release by ouabain. | the effect of ouabain, a specific sodium-potassium dependent adenosine triphosphatase (na+-k+-atpase) inhibitor, on antigen-induced histamine release was studied using guinea pig lung fragments sensitized in vitro with rabbit antibodies against bovine serum albumin. histamine was assayed spectrofluorometrically. when sensitized tissue had been preincubated with ouabain (less than or equal to 1.0 x 10(-4) m) for 10 min prior to antigenic challenge, release of histamine was significantly inhibited ... | 1976 | 57130 |
| a canine distemper model of virus-induced anergy. | for development of an animal model of virus-induced anergy, the effect of canine distemper virus (cdv) upon cell-mediated immunity in dogs was investigated. first, canine cutaneous reactions and in vitro lymphocyte responses to soluble protein antigens were characterized. dogs immunized with picryl guinea pig albumin and with keyhole limpet hemocyanin (both in complete freund's adjuvant) responded reproducibly to intracutaneous challenge with these antigens. reactivity peaked in 20-40 days (maxi ... | 1976 | 57199 |
| qualitative and quantitative studies on human myelin basic protein in situ with respect to time interval between death and autopsy. | normal human frontal lobe white matter obtained at autopsy was used to determine the extent of in situ post-mortem degradation of myelin basic protein (bp). effects of the following two factors were studied: 1) time interval between death and autopsy, and 2) freezing and thawing the tissue. quantitative extraction of bp from the autopsy material showed only minimal loss of bp that could be attributed to the time interval between death and autopsy (up to 48 h). the purified bp from these samples ... | 1976 | 57210 |
| enhancement of encephalitogenic activity by the formation of myelin basic protein-brain acidic protein complex. | encephalitogenic protein fraction (bec) was isolated from bovine brain tissue by extraction with salt-ethanol mixture at neutral ph, instead of employing dilute mineral acid. the fraction bec was separated into two fractions. an acid-soluble protein was encephalitogenic and the major component was very alike to the basic protein of myelin (al). the other was acid-insoluble acidic protein that was not encephalitogenic even at a dose of 100 mug. the acidic protein formed an insoluble complex with ... | 1975 | 57252 |
| dna polymerase activity in plasma from leukaemic guinea-pigs. | measurement of dna polymerase in leukaemic guinea-pig plasms reveals the presence of low levels of sedimentable and non-sedimentable enzymic activities. since the sedimentable dna polymerase is ribonuclease sensitive, uses poly(c).oligo(dg) as template, and bands in a sucrose density gradient at 1-17 g/ml it is thought to be the gplv-associated reverse transcriptase. the soluble dna polymerase is stimulated by ribonuclease and is probably of cellular origin. | 1976 | 57790 |
| shared antigens between bacteria and guinea pig line 10 hepatocarcinoma cells. | this study was undertaken to investigate the possibility that listeria monocytogenes, brucella abortus, and salmonella typhimurium share antigenic components with guinea pig line 10 hepatocarcinoma cells. rabbits were immunized with sonicates of these bacteria or line 10 tumor cells. other rabbits were immunized with line 1 cells, a tumor with antigenic characteristics different from those of line 10. the binding of antibodies to radiolabeled antigens prepared from extracts of bacteria and line ... | 1976 | 57823 |
| blocking the encephalitogenic activity of the c-terminal part of the basic myelin protein in rabbits by treatment with serum. | peptides hnb-92-169 and 116-169, tyr, derivatives of the c-terminal part of bovine myelin basic protein, sensitize rabbits for experimental allergic encephalomyelitis (eae). dissolving these peptides in heat-inactivated normal guinea-pig, human, or monkey serum immediately before emulsification with freund's complete adjuvant (fca) abrogated their disease-inducing activity. dissolving them in homologous or autologous serum only slightly affected the encephalitogenicity. | 1976 | 57920 |
| carboxylic ester hydrolases in the thyroid gland of the guinea-pig. a light microscopic study. | the location of cholinesterase and non-specific esterase in the thyroid gland of the guniea-pig was studied with the light microscope. it was found that the idoxyl method for non-specific esterase activity under special conditions is superior to the cholinesterase method in a number of respects for the demonstration of the intra-, inter- and parafollicular cells. when using the indoxyl method the incubation period can be reduced from 2.5-3 hr to 40-50 min. further, the reaction can be followed d ... | 1976 | 57946 |
| tannic acid effect on membrane of cell surface origin in guinea pig megakaryocytes and platelets. | plasma membranes in isolated guinea pig megakaryocytes and washed platelets are poorly stained with the usual methods used to outline cell membranes. the addition of tannic acid and calcium to the initial fixative is useful to enhance electron density of all surface-derived membrane systems in these cells. the method described here shows that the increased electron denisty of membrane after fixation in the presence of tannic acid occurs both at the cell surface and along the invaginated membrane ... | 1976 | 58024 |
| shared antigens between human malignant melanoma cells and mycobacterium bovis (bcg). | this study was undertaken to investigate the antigenic relationships between human malignant melanoma cells and mycobacterium bovis (bcg). rabbits were immunized with sonicates of bcg or with malignant melanoma cells from different patients and the resulting antisera were tested for their capacity to bind radiolabeled soluble extracts prepared from bcg and melanoma cells. the binding of antibodies to radiolabeled antigens was studied by precipitation of radiolabeled antigen-antibody complexes by ... | 1976 | 58033 |
| studies on autoimmune encephalomyelitis in the guinea pig. ii. an in vitro investigation on the nature, properties, and specificity of the serum-demyelinating factor. | complement-dependent demyelinating activity of whole brain homogenate (wbh)-induced experimental allergic encephalomyelitis (eae) sera was tested on long term tissue cultures of in vitro myelinated fetal guinea pig cerebellum. complement-fixing (cf) auto-antibodies were shown to be the responsible agents, as demonstrated in experiments where all reagents belonged to the same species: guinea pigs of outbred (hartley) and even of inbred (s2 or s13) strains. these antibodies were of the igg2 class ... | 1976 | 58035 |
| the inhibition of cutaneous basophil hypersensitivity reactions by a heterologous anti-guinea pig t cell serum. | systemic treatment with a heterologous anti-t cell serum of guinea pigs immunized with ea in ifa markedly suppressed cbh reactivity to specific antigen and t cell mitogens, as judged by gross reactivity, histology, and skin histamine. the antiserum produced a marked drop in circulating lymphocytes, mainly at the expense of t cells, as indicated by the ability of surviving lymphocytes to rosette with rabbit rbc. it was postulated that the suppression of cbh reactivity is due to the depletion of t ... | 1976 | 58042 |
| characterization of the oncornavirus particles in the plasma of guinea pigs with l2c leukemia. | the inoculation of l2c guinea pig leukemia cells into strain 2 guinea pigs results in the death of the animals within 12 to 15 days. death is preceded by the simultaneous appearance in the plasma of (i) elevated leukocyte levels, (ii) extracellular virus particles, and (iii) a particle-associated rna-directed dna polymerase. this enzyme activity has a cation preference identical to that of the type b bromodeoxyuridine-induced guinea pig virus, i.e., an mg2+ optimum at 20 mm and no activity using ... | 1976 | 58078 |
| the specificity of t lymphocyte responses to chemically defined antigens. | a system is described that allows the definition of t cell receptor specificity with some precision. it involves immunization of guinea pigs with hapten coupled to mycobacteria. the t cells of such animals respond to many but not all carriers modified by that hapten. such t cells recognize neither hapten nor carrier alone, but rather determinants involving both the hapten and the carrier. no evidence for hapten-specific t cells was found. a model of the antigen binding site of the t cell recepto ... | 1976 | 58456 |
| induction of tolerance in adult guinea-pigs by two antigen dosage levels and by antigenic competition. | the effect of prior injection of increasing doses of dinitrophenylated bovine serum albumin (dnp-bsa) mixed in freund's complete (fca) or incomplete (ifa) adjuvants on the antibody response to a subsequent challenge with an optimal immunizing dose of dnp-bsa in fca has been examined and compared with the effect of competition with the unrelated antigen bovine gamma-globulin (bgg). an unresponsive state was induced by pretreatments with either a minimal dose of antigen, a large antigen excess or ... | 1975 | 58574 |
| the specific binding of radioiodinated vasoactive intestinal octacosapeptide (vip) on isolated pancreatic acinar cells from the guinea pig. | | 1975 | 58613 |
| [study of prostaglandin synthesis during anaphylactic shock in guinea pigs]. | in guinea pigs actively sensitized with ovalbumin and shocked, we have measured blood histamine and histaminase, pulmonary content in cycli amp and prostaglandins (pg) e1, e2 and f2alpha. in pulmonary efferent blood, both pge and pgf are increased. in lung tissue, only pgf2alpha, which are bronchoconstructive mediators, are increased. th increased level in pg seems to have two origins: first a specific increase, then an increased synthesis secondary to histamine release. | 1976 | 58740 |
| f-antigen: nature, liver specificity, and release in experimental liver injury. | f-antigen is a liver-specific antigen detected with antibody, raised in allogeneic (cba) mice, to balb/c mouse liver extract. the authors have confirmed the hepatic specificity of this antigen by showing it to be absent in extracts of extrahepatic organs of mouse and human origin. it is present in liver extracts of guinea pigs, rats, and rabbits, as well as in liver extracts from mouse and human sources. the antigen was present in the circulation of rabbits with acute carbon tetrachloride-induce ... | 1976 | 58803 |
| hapten-specific responses to contact sensitizers. use of fluorodinitrobenzene to elicit migration inhibition and macrophage agglutination factors from lymph node cells of contact-sensitive guinea-pigs. | hapten-specific sensitivity of guinea-pigs sensitized to dinitrophenyl (dnp) contactants and to dnp-protein conjugates was investigated by skin test and by antigen-induced elaboration of migration inhibition (mif) and macrophage agglutination factors (maf) from lymph node cells. the delayed contact reaction was highly specific for low doses of contactant and markedly less so for conjugates; lymph node cells elaborted both lymphokines in response to brief exposures to dinitrofluorobenzene (dnfb) ... | 1976 | 58832 |
| reactive and nonreactive lymphocytes in experimental allergic encephalomyelitis. i. possible role of macrophage-lymphocyte interactions. | previous studies have shown that peritoneal inflammatory exudate cells from guinea pigs with experimental allergic encephalomyelitis proliferate prominently when cultured with the sensitizing myelin basic protein. peripheral blood lymphocytes (pbl) obtained at the same time responded little, if at all. in the present studies, recombination experiments using appropriate mixtures of peritoneal exudate macrophages and pbl show that the presence of such macrophages will not enhance in vitro reactivi ... | 1976 | 58839 |
| presence and anatomic location of species and organ-specific intestinal antigens. | several groups of antigens were detected in the ileum of rabbits, rats, and guinea pigs. an antigen present in the cytoplasm of the epithelial cells is shared by these species, but is not tissue-specific because it can be absorbed by the liver. organ-specific ileum antigens are present in the goblet cell mucus. there are different ileum-specific antigens in the three species and they do not elicit autoantibody formation. additional antigens are located in the basement membrane and some basement ... | 1976 | 58841 |
| protection against experimental allergic encephalomyelitis with peptides derived from myelin basic protein: presence of intact encephalitogenic site is essential. | experimental allergic encephalomyelitis (eae) is a cell-mediated autoimmune response directed toward a component of central nervous system (cns) tissue, myelin basic protein (bp). injection of animals with either whole cns tissue or purified bp in complete freund's adjuvant (cfa) induces severe and usually fatal disease. preimmunization of animals with bp in incomplete freund's adjuvant (ifa) prevents eae. we have examined the relative abilities of whole guinea pig bp and its fragments to protec ... | 1976 | 58930 |
| the effect of antigen suicide on numbers of cells binding defined antigenic determinants. | lymph node cells from guinea pigs immunized to oxidized ferredoxin (o-fd) were treated in an antigen suicide procedure designed to inactivate lymphocytes binding the haptenic peptide determinants of the ferredoxin molecule. o-fd-induced dna synthesis and cells binding either peptide determinant were examined both before and after allowing suicide. the proliferative response to o-fd and the number of determinant-binding cells were specifically and markedly decreased after antigen suicide. | 1976 | 58940 |
| inhibition of hypersensitivity reactions by soluble derivatives of baicalein. | baicalein, a flavonoid, is anti-allergic but only slightly soluble in water. the soluble derivatives of baicalein, disodium baicalein-6-phosphate (bps) and sodium baicalein-6-sulfate (bss), were synthesized and examined regarding their effects on hypersensitivity reactions. these derivatives inhibited type i and ii reactions as classified by coombs and gell. the arthus reaction belonging to type iii reaction, however, was hardly affected with either bps or bss. the experimental asthma caused by ... | 1975 | 59001 |
| [active immunization against tetanus and its evaluation]. | the introduction of 5 ml. tetanus toxoid into guinea pigs stimulates the production of antibodies. the new specific substance can be measured invivo and invitro. in our tests 30 guinea pigs of 300-350 gr. were used. before and after toxoid injections blood samples were taken and the sera were separated. in electrophoretic studies of the sera 5 fractions; albumin, alpha 1, alpha 2, beta and gamma globulins were noticed. the value of fractions and the statistical estimation are shown in tables 1-2 ... | 1976 | 59305 |
| the effect of neuraminidase on the sensitivity of tumor cells toward lysis by antibody and complement or by sensitized lymphocytes. | the effect of neuraminidase treatment on the antigenicity of sarcoma i (sal) and mastocytoma p-815-x2 cells was compared, both in terms of their reaction with various antisera (c57bl/6 anti-sai, c57bl/6 anti-mastocytoma, rabbit and horse als) and complements (guinea pig, rabbit) or with sensitized lymphocytes (c57bl/6 anti-sai, c57bl/6 anti-mastocytoma, a anti-mastocytoma). with sensitized lymphocytes the reactivity of neuraminidase-treated tumor cells was similar to that of nontreated cells. in ... | 1976 | 59438 |
| specificity of antigenic fractions of tuberculin. | purified protein derivative from mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 v/40 ma in ph 4.2 acetic acid-pyridine buffer. further purification of one of the fractions on sephadex g 25 column and by acid hydrolysis yielded antigen "ps" eliciting tuberculin reaction only in animals vaccinated with m. bovis bcg but not in those sensitized with m. avium and some fast-growing mycobacteria. the specificity of antigen "ps" was confirmed in vitro: the antigen induced ... | 1976 | 59518 |
| non-specific antimicrobial cellular resistance in sensitized guinea pigs. | specific delayed-type hypersensitivity was induced in guinea pigs with bovine albumin + complete freund adjuvant, bovine gamma globulin + complete freund adjuvant and bcg vaccine. the animals were subsequently tested for nonspecific antimicrobial resistance. sensitized and control groups were challenged intraperitoneally with listeria monocytogenes 2 hr after reinjection with the sensitizing antigen. the listeria content of the spleens was determined 1 or 5 days after the infection. the number o ... | 1976 | 59519 |
| aspects of the secondary antibody response to ox insulin in the hartley guinea-pig; the use of chemically modified ox insulin to delineate the antigenic determinants of ox insulin. | the use of a quinea-pig model to study the immunogenicity of the insulin molecule is presented. the hartley guinea-pig has been shown consistently to form antibody to ox insulin, when given in a water-in-oil emulsion containing pertussis vaccine as adjuvant. after log transformation of standardized antibody titres to iodo-ox insulin, a valid statistical comparison of the antibody response to different ox insulin preparations could be made. antibody cross-reacting with ox insulin, but not iodo-ox ... | 1976 | 59648 |
| determinant competition during the immune response to n-acyl derivatives of ox insulin in the hartley guinea-pig. | subgroups of female hartley guinea-pigs were immunized with n-carbamylated ox insulin, n-maleylated ox insulin, n-phthaloylated ox insulin, or with the crystalline ox insulin from which the n-acylated insulins had been prepared. the immunogens were administered in water-in-oil emulsions containing pertussis vaccine as adjuvant. sera obtained 20 days after secondary immunization were assayed for their antibody titres to iodo-ox insulin and their insulin-binding capacities. the data were log trans ... | 1976 | 59652 |
| immunological investigations of antigenicity and specificity of soluble collagen fractions. iv. anaphylaxis and allergy experiments. | collagen prepared from rat, calf, or pig skin was tested on guinea pigs for active cutaneous anaphylaxis. only one fraction of calf skin collagen gave a slight, positive reaction. no positive reactions were observed in 21 subjects submitted to patch tests and 12 subjects submitted to scratch tests with pig skin collagen. therefore preparations of pig skin collagen may be used for wound treatment without any risk. | 1976 | 59667 |
| modulation of cellular-immune responses in vivo and in vitro by histamine receptor-bearing lymphocytes. | histamine, one of the mediators involved in the ige-mediated reaction, was demonstrated to influence in vivo and in vitro components of cellular-immune reactions in orthochlorbenzoyl-bovine gamma globulin-immune guinea pigs. 10(-3) m histamine reduced by half the size of a delayed hypersensitivity skin test at 24 h. inhibition of skin reactivity by histamine could be partially reversed by h-1 receptor antagonists such as chlorpheniramine and completely prevented by h-2 receptor antagonists such ... | 1976 | 59738 |
| cross-reactive t-cell antigens among mammalian species. | fluoresceinated heteroantisera prepared against t cells of rats, monkeys, and humans were reacted withthymus and spleen cells from 11 selected species. these reagents recognized cross-reacting t cell antigen(s) among rodent species (mouse, rat, guinea pig, and hamster) and among primate species (monkey and humans). with one exception, the cross-reactivity was restricted to a phylogenetic order. all three antisera required relatively few absorptions to achieve t cell specificity for related speci ... | 1976 | 59778 |
| naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. | a new class of murine leukemia viruses, isolated from wild mus musculus trapped in california, is described. these viruses, designated "amphotropic," replicate in mouse, rabbit, mink, human, guinea pig, and rat cells, but not in hamster, quail, or duck cells. they show n-tropism for mouse cells, and do not trigger the xc cell response. they are distinct by interference and virus neutralization testing from the previously recognized mouse-tropic and xenotropic mulv classes. mouse-tropic viruses o ... | 1976 | 59816 |
| interaction of human waldenström's igm proteins with guinea pig and human complement. | the activity of purified human waldenström's igm protein to fix complement of human and guinea pig origins was compared at different temperatures using the polystyrene latex particle-adsorption method. it was shown that the interaction of the igm proteins with complement differed depending on the source of complement and that a pronounced heterogeneity in complement-fixing activity was observed among the igm proteins when tested with guinea pig complement. thus, by the use of guinea pig compleme ... | 1976 | 59830 |
| experimental studies on drug sensitization. ii. production of antiacetylsalicylyl antibodies and their detection by passive hemagglutination. | antiacetylsalicylyl antibodies were produced in rabbits and guinea-pigs by innoculation of acetylsalicylic acid incorporated in freund adjuvant. these antibodies were readily detected by a passive hemagglutination test using rabbit erythrocytes incubated with acetylsalicylic acid at alkaline ph. acetylsalicylyl conjugates with human gammaglobulin, bovine gammaglobulin and rabbit gammaglobulin were also prepared by incubating the proteins with acetylsalicylic acid at alkaline ph. in parallel expe ... | 1976 | 59932 |
| guinea-pig ia antigens: functional significance and chemical characterization. | | 1976 | 59972 |
| guinea pig histocompatibility antigens. i. serological and genetic characterization of gpla antigens. | lymphocytotoxic alloantisera were obtained by cross immunization among outbred guinea pigs from a closed colony. groups of antisera recognizing the same specificities were established by panel analysis, cross immunization among appropriate phenotypes, and absorption studies. three antigens designated b1, b2, and b3 (perviously b, c, and d) were detected in our outbred colony and also found to be present in a wide variety of guinea pig strains. these antigens were shown by population and breeding ... | 1976 | 59983 |
| an evaluation of delayed hypersensitivity in guinea pigs to various trichophytin preparations. | guinea pigs immunized with mycelium from trichophyton mentagrophytes in freund's complete adjuvant were tested intradermally with the following trichophytin preparations: tri extracted by ethylene glycol, trii extracted by phenol and trii, a commercially obtained preparation fromsächsisches serumverk kg. in our experience, tri was found to be superior to the other preparations. it showed signifiicant delayed skin reactions in all sensitized animals and none in the controls. lymphocyte stimulatio ... | 1976 | 60024 |
| cross-sensitivity of common aminoglycoside antibiotics. | guinea pigs were sensitized to neomycin (a, b, or c), paromomycin, gentamicin, kanamycin, streptomycin, and dihydrostreptomycin via intradermal or foot-pad injection with an adjuvant containing killed mycobacterium butyricum or m tuberculosis h37ra (ra). these antibiotics produced greater cross-sensitization with an increase in the number of immunizations and chemical structural similarities. after repeated intradermal injections (adjuvant ra) of neomycin, guinea pigs showed cross-sensitization ... | 1976 | 60083 |
| chlorpromazine-induced histamine release from guinea-pig skin in vitro--a photosensitive reaction. | chlorpromazine releases histamine from chopped guinea-pig skin maintained in vitro at 37 degrees c. this effect was markedly potentiated by radiant energy. this potentiation increased with increasing exposure to radiant energy. the possible clinical applications of these findings are discussed. | 1976 | 60086 |
| immunochemical studies of proteins in epidermal cornified cells of human and newborn rat. | 1. proteins were extracted from cornified cells of newborn rats and human palm with 8 m urea containing 0.1 m beta-mercaptoethanol. two fractions, rat fiiia and human f5.5 were obtained by acid precipitation for further study. 2. antibodies raised in rabbits by injection of rat fiiia gave two precipitin lines by agarose diffusion against rat fiiia, but only one line against human f5.5. one of the antigenic determinants of rat fiiia was found to be a protein of approximately 66 000 daltons. the o ... | 1976 | 60135 |
| hemagglutination of neuraminidase-treated human erythrocytes by leishmania enriettii infected guinea-pig sera. | sera from guinea-pigs infected with the protozoan parasite leishmania enriettii showed higher hemagglutination (ha) titres for neuraminidase treated human erythrocytes than those of normal guinea-pig sera. this ha activity was associated mostly with the 19s fraction of the immune serum and could be absorbed out with an antigenic fraction of the parasite membrane. antigenic determinants involved in this ha reaction consisted of, at least, beta-d-galactosyl or lactosyl residues. | 1976 | 60254 |
| formed blood elements in peritoneal effusion and the macrophage migration inhibition test in healthy guinea pigs and in guinea pigs with experimental allergic encephalomyelitis. | an attempt was made to correlate the percentages of macrophages, lymphocytes and granulocytes in the peritoneal effusion in healthy guinea pigs and guinea pigs with experimental allergic encephalomyelitis (eae), with the macrophage migration inhibition (mmi) test. varying percentages of the cells had no influence on values of mmi. similarly, in guinea pigs with eae, percentages of formed elements in peritoneal effusion were not correlated with intensity of mmi or with histopathologic lesions in ... | 1976 | 60984 |
| [necroses in the granular cell layer of the cerebellum due to methylchloride intoxication in guinea pigs (author's transl)]. | neurotoxic action of methylchloride was reported by various authors. as this solvent is widely used in chemical industry the morphological changes in the cns of guinea pigs due to methylchloride intoxication were comparatively studied by light- and electron-microscopy. | 1975 | 61130 |
| the effect of edta and metal cations on the 5-bromoindoxyl acetate esterase activity in the thyroid of the guinea pig. | miscellaneous metal cations and edta have been used as activators and inhibitors of esterase activity in the thyroid of the guinea-pig. the results indicate that the 5-bromoiondoxyl acetate esterase in the epithelial cells probably consists of two different a-esterase isoenzymes, one present in group i cells (the para-, intra-, and inter-follicular cells) and the other in group ii cells (the follicular cells proper). the first isoenzyme seems to be calcium-dependent whereas the other is activate ... | 1976 | 61192 |
| mandibular condylectomy in the guinea pig: quantitative triple fluorochrome study. | different-colored fluorochromes were administered sequentially to guinea pigs and the rate of bone formation measured in their condylectomized control contralateral hemimandibles. the values for labeled bone for condylectomized hemimandibles were lower than for the control contralateral hemimandibles of the same guinea pig. the differences in values of condylectomized and control contralateral sides for dctc- and total surface area-labeled bone were statistically significant, but were not statis ... | 1976 | 61214 |
| the major histocompatibility complex of the guinea-pig. ii. relationship between mixed leucocyte reactivity and serologically-defined phenotypes of the gpla b locus and i region. | the relationship between the mixed leucocyte reaction (mlr) and the serologically-defined antigens controlled by genes in the guinea-pig b locus (equivalent to the murine d or k region) and i region was investigated in various inbred and partially inbred strains and in guinea-pig families homozygous for their gpla alleles. no mlr reactions could be detected among guinea-pig families of a closed colony which had been bred to homozygosity for their gpla antigens. by contrast, animals which differe ... | 1976 | 61245 |