| cortisol and parathyroid hormone-related peptide are reciprocally modulated by negative feedback. | in previous in vitro studies, we have shown that the n-terminal region of parathyroid hormone-related protein (pthrp) can stimulate cortisol production in sea bream, sparus auratus, interrenal tissue, possibly through a paracrine action. in the current study, the systemic interaction between cortisol and pthrp was studied in vivo. sustained elevated blood cortisol levels, induced either by cortisol injection or confinement stress, suppressed circulating pthrp 6 and 24-fold, respectively, by comp ... | 2006 | 16624313 |
| vegetable lipid sources affect in vitro biosynthesis of triacylglycerols and phospholipids in the intestine of sea bream (sparus aurata). | despite the good growth performance of several fish species when dietary fish oil is partly replaced by vegetable oils, recent studies have reported several types of intestinal morphological alterations in cultured fish fed high contents of vegetable lipid sources. however, the physiological process implied in these morphological changes have not been clarified yet, since alterations in the physiological mechanisms involved in the different processes of lipid absorption could be responsible for ... | 2006 | 16512929 |
| intra-adrenal interactions in fish: catecholamine stimulated cortisol release in sea bass (dicentrarchus labrax l.). | the effect of the catecholamines, adrenaline and noradrenaline, on sea bass (dicentrarchus labrax) and sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system technique. increasing concentrations of catecholamines (10(-6), 10(-8) and 10(-10) m) stimulated cortisol production in a dose-dependent manner, in sea bass only. the increase in cortisol production stimulated by adrenaline (10(-6) m) and noradrenaline (10(-6) m) was inhibited by so ... | 2006 | 16443381 |
| food deprivation alters osmoregulatory and metabolic responses to salinity acclimation in gilthead sea bream sparus auratus. | the influence of acclimation to different environmental salinities (low salinity water, lsw; seawater, sw; and hyper saline water, hsw) and feeding conditions (fed and food deprived) for 14 days was assessed on osmoregulation and energy metabolism of several tissues of gilthead sea bream sparus auratus. fish were randomly assigned to one of six treatments: fed fish in lsw, sw, and hsw, and food-deprived fish in lsw, sw, and hsw. after 14 days, plasma, liver, gills, kidney and brain were taken fo ... | 2006 | 16432730 |
| arginine vasotocin (avt) and isotocin (it) in fish brain: diurnal and seasonal variations. | an hplc assay with solid-phase extraction and fluorescence derivatization was developed for measurement of arginine vasotocin (avt) and isotocin (it) in the neural tissues of fish. the efficiency and usefulness of the method have been verified in experiments by examination of peptides concentrations in brains of three fish species. the day-night changes in neuropeptides levels have been studied in brains of adult sea bream (sparus aurata) and juvenile atlantic salmon (salmo salar). seasonal fluc ... | 2006 | 16413807 |
| toxicity and gross pathology of ivermectin bath treatment in sea bream sparus aurata, l. | the isopod parasite ceratothoa oestroides becomes hard to eradicate in intensive fish rearing systems and in addition to causing weight loss in adult fish it causes mortalities in larval and juveniles stages of sea bass and sea bream. faced with the fast emergence of this infection in adriatic finfish facilities, an effective compound had to be tested for bath treatment. ivermectin was chosen for this purpose on the basis of extensive practice in northern european countries which used ivermectin ... | 2006 | 16406587 |
| time course of osmoregulatory and metabolic changes during osmotic acclimation in sparus auratus. | changes in different osmoregulatory and metabolic parameters over time were assessed in gills, kidney, liver and brain of gilthead sea bream sparus auratus transferred either from seawater (sw, 38 p.p.t.) to hypersaline water (hsw, 55 p.p.t.) or from sw to low salinity water (lsw, 6 p.p.t.) for 14 days. changes displayed by osmoregulatory parameters revealed two stages during hyperosmotic and hypo-osmotic acclimation: (i) an adaptive period during the first days of acclimation (1-3 days), with i ... | 2005 | 16272252 |
| identification of estrogen-responsive genes in the testis of sea bream (sparus auratus) using suppression subtractive hybridization. | there is growing evidence that estrogens play important roles in both normal and xenoestrogen disrupted testis physiology. however, the mechanisms and signaling pathways involved, in particular in fish, are largely unknown. we have used suppression subtractive hybridization to isolate 152 candidate estrogen-responsive genes in the testis of male estradiol (e2)-treated sea bream (sparus aurata). the e2 up-regulation of some of the genes (e.g., choriogenin l and h, vitellogenin i and ii, apolipopr ... | 2006 | 16267841 |
| food-deprivation induces hsp70 and hsp90 protein expression in larval gilthead sea bream and rainbow trout. | heat shock proteins (hsps) expression is commonly used as indicators of cellular stress in animals. however, very little is known about either the expression patterns of hsps or their role in the stress-tolerance phenomenon in early life stages of fish. to this end, we examined the impact of food-deprivation (12 h), reduced oxygen levels (3.5 mg/l for 1 h) and heat shock (hs: +5 degrees c for 1 h) on hsp70 and hsp90 protein expression in early life stages of the gilthead sea bream (sparus aurata ... | 2005 | 16257553 |
| characterization of estrogen receptor betab in sea bream (sparus auratus): phylogeny, ligand-binding, and comparative analysis of expression. | estrogens control many physiological processes in both female and male vertebrates, mostly mediated by specific nuclear estrogen receptors (er). two er subtypes (eralpha and erbeta) are present in most vertebrates, including the sea bream (sparus auratus) a hermaphrodite teleost fish. in the present study several variant cdnas encoding a second sea bream erbeta (sberbetab) is reported. phylogenetic and southern blot analysis indicate that sberbetab and the previously cloned sberbetaa (formerly s ... | 2006 | 16213504 |
| ligand binding and signalling pathways of pth receptors in sea bream (sparus auratus) enterocytes. | whole animal studies have indicated that ca(2+) uptake by the gastrointestinal tract is regulated by the action of parathyroid hormone-related peptide (pthrp) in teleost fish. we have characterised pth receptors (pthr) in piscine enterocytes and established, by using amino-terminal pthrp peptides, the amino acid residues important for receptor activation and for stabilising the ligand/receptor complex. ligand binding of (125)i-(1-35(tyr)) pthrp to the membrane fraction of isolated sea bream ente ... | 2006 | 16189716 |
| calcium mobilization from fish scales is mediated by parathyroid hormone related protein via the parathyroid hormone type 1 receptor. | the scales of bony fish represent a significant reservoir of calcium but little is known about their contribution, as well as of bone, to calcium balance and how calcium deposition and mobilization are regulated in calcified tissues. in the present study we report the action of parathyroid hormone-related protein (pthrp) on calcium mobilization from sea bream (sparus auratus) scales in an in vitro bioassay. ligand binding studies of piscine 125i-(1-35(tyr))pthrp to the membrane fraction of isola ... | 2005 | 16181689 |
| identification and analysis of teleost slow muscle troponin t (stnt) and intronless tnt genes. | in the present study cdna clones representing two slow skeletal muscle troponin t genes (stnt1sb and stnt2sb) in the sea bream (sparus auratus), an important aquaculture species, were isolated and characterised. a third, intronless, tnt gene (itntsb), which is an apparent orthologue of a previously described zebrafish tnt, was also isolated. in adult sea bream stnt expression was restricted to red muscle and, using northern blotting, a single low abundance transcript was identified for stnt1sb ( ... | 2005 | 16168583 |
| interactive effects of high stocking density and food deprivation on carbohydrate metabolism in several tissues of gilthead sea bream sparus auratus. | the influence of high stocking density (hsd) and food deprivation was assessed on carbohydrate metabolism of several tissues of gilthead sea bream sparus auratus for 14 days. fish were randomly assigned to one of four treatments: (1) fed fish under normal stocking density (nsd) (4 kg m(-3)); (2) fed fish under hsd (70 kg m(-3)); (3) food-deprived fish under nsd; and (4) food-deprived fish under hsd. after 14 days, samples were taken from the plasma, liver, gills, kidney and brain for the assessm ... | 2005 | 16106404 |
| gene expression profiling of gilthead sea bream during early development and detection of stress-related genes by the application of cdna microarray technology. | large-scale gene expression studies were performed for one of the main european aquaculture species, the gilthead sea bream sparus auratus l. for this purpose, a cdna microarray containing 10,176 clones from a cdna library of mixed embryonic and larval stages was constructed. in addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. however, so far, no lar ... | 2005 | 16046618 |
| branchial osmoregulatory response to salinity in the gilthead sea bream, sparus auratus. | the branchial osmoregulatory response of gilthead sea bream (sparus auratus l.) to short-term (2-192 hr) and long-term (2 weeks) exposure to different environmental salinities (5 per thousand, 15 per thousand, 25 per thousand, 38 per thousand and 60 per thousand) was investigated. a "u-shaped" relationship was observed between environmental salinity and gill na+,k+ -atpase activity in both long- and short-term exposure to altered salinity, with the increase in activity occurring between 24 and 9 ... | 2005 | 15945079 |
| evaluation of gilthead sea bream, sparus aurata, sperm quality after cryopreservation in 5 ml macrotubes. | cryopreservation produces several types of damage in spermatozoa, leading to fertility impairment. the reduction arises both from a lower viability post-thaw and from sublethal dysfunctions in some of the surviving cells. in the present study, we have analysed the effect of cryopreservation in 5 ml macrotubes on the quality of post-thawed gilthead sea bream sperm. several standard sperm quality parameters were determined: ph and osmolarity of seminal plasma, sperm concentration, and motility. an ... | 2005 | 15925579 |
| parasite communities of adriatic cage-reared fish. | from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ... | 2005 | 15900691 |
| sea bream (sparus auratus) estrogen receptors: phylogeny and tissue distribution. | most estrogen actions are mediated by two estrogen receptor subtypes (eralpha and erbeta). while a single erbeta appears to be present in higher vertebrates, two forms of erbeta encoded by different genes have recently been isolated in some teleost fish species. we investigated whether this also applies to the hermaphrodite sparid sea bream (sparus auratus) and cloned a second erbeta (sberbeta2) in this species. we have also compared the tissue distribution of the three receptors as yet identifi ... | 2005 | 15891082 |
| the involvement of thyroid hormone metabolism in gilthead sea bream (sparus auratus) osmoregulation. | we have investigated the effect of adaptation to low salinity water on the thyroid status of the euryhaline teleost, sparus auratus. we show that, following low salinity adaptation, the plasma t(4) concentration increases and branchial deiodination activities of t(4), t(3), and rt(3) decrease. moreover, branchial and hepatic enzyme activities that are putatively involved in thyroid hormone metabolism respond differentially in low salinity conditions. our results indicate the involvement of thyro ... | 2005 | 15891062 |
| duplication of growth hormone receptor (ghr) in fish genome: gene organization and transcriptional regulation of ghr type i and ii in gilthead sea bream (sparus aurata). | nucleotide sequences encoding for functional growth hormone receptors (ghr) are now available in salmonids (coho and masu salmon) and other fish orders. several authors have hypothesized a divergent evolution of salmonid ghrs, but the recent finding that a trout cdna is related to non-salmonid (ghr type i) rather than to salmonid ghrs (ghr type ii) points out a possible duplication of actively transcribed ghr genes. to address this issue, we search by rt-pcr for ghr type ii in trout, gilthead se ... | 2005 | 15862563 |
| ontogeny of osteonectin expression in embryos and larvae of sea bream (sparus auratus). | osteonectin (osn) is a glycoprotein which is implicated in development, bone formation and mineralisation, tumorigenesis, angiogenesis, and wound healing. regulation of its expression by hormones may be one of the mechanisms by which the endocrine system affects bone metabolism. as a first step to understanding osn function in fish, the gene expression of the recently cloned cdna for sea bream, sparus auratus, osteonectin (sbosn) was characterised during embryonic and larval development. sbosn m ... | 2005 | 15862559 |
| evaluation of dna damage in rainbow trout (oncorhynchus mykiss) and gilthead sea bream (sparus aurata) cryopreserved sperm. | cryopreservation causes several types of damage to spermatozoa, such as loss of plasma membrane integrity and functionality, loss of motility, and atp content, resulting in decrease of fertility rates. this spermatozoal damage has been widely investigated for several marine and freshwater fish species. however, not much attention has been paid to the nuclear dna. the objective of this study was to determine the degree to which cryopreservation induces spermatozoal dna damage in two commercially ... | 2005 | 15843004 |
| comparison of volatiles of cultured and wild sea bream (sparus aurata) during storage in ice by dynamic headspace analysis/gas chromatography-mass spectrometry. | cultured and wild sea bream were compared for differences in their volatile components over a 23 day storage period in ice. a total of 60 compounds in cultured and 78 compounds in wild sea bream were tentatively identified (in addition to this, there were 23 unknowns in cultured and 29 unknowns in wild sea bream volatiles). these included aldehydes, ketones, alcohols, aromatics, terpenes, furans, sulfur-containing compounds, an acid, and miscellaneous compounds. although selection of best fish i ... | 2005 | 15796602 |
| three peroxisome proliferator-activated receptor isotypes from each of two species of marine fish. | the cloning and characterization of cdnas and genes encoding three peroxisome proliferator-activated receptor (ppar) isotypes from two species of marine fish, the plaice (pleuronectes platessa) and the gilthead sea bream (sparus aurata), are reported for the first time. although differences in the genomic organization of the fish ppar genes compared with their mammalian counterparts are evident, sequence alignments and phylogenetic comparisons show the fish genes to be homologs of mammalian ppar ... | 2005 | 15790725 |
| isolation and characterization of piscine osteonectin and downregulation of its expression by pth-related protein. | the skeleton is the main source of osteonectin mrna in adults of the seawater teleost sea bream sparus auratus. it is expressed by cells forming the basement membrane of calcifying tissue indicating that, as in mammals, it may play a role in osteoblast differentiation. pthrp induced downregulation of osteonectin mrna in vitro in scales, a mineralizing tissue with bone-like metabolism. this indicates a means to redirect calcium to activities such as vitellogenesis when this ion is in high demand. | 2005 | 15765188 |
| hormonal regulation of hepatic igf-i and igf-ii gene expression in the marine teleost sparus aurata. | the present work aimed to determine whether gnrh potentiates the effect of growth hormone (gh) on insulin-like growth factors (igf-i and igf-ii) hepatic gene expression in sparus aurata liver. since several hepatic genes were shown to underlie direct regulation via the hepatic estrogen receptor, another aim was to extend our understanding of direct estrogen effects on liver igfs gene expression. pre-reproductive sea bream females were treated with gh, gnrh, estradiol-17beta, gh plus gnrh, and es ... | 2005 | 15736123 |
| na-dependent d-glucose transport by intestinal brush border membrane vesicles from gilthead sea bream (sparus aurata). | brush border membrane vesicles (bbmv) enriched in sucrase, maltase and alkaline phosphatase, and impoverished in na(+)-k(+)-atpase, were isolated from proximal and distal intestine of the gilthead sea bream (sparus aurata) by a mgcl(2) precipitation method. vesicles were suitable for the study of the characteristics of d-glucose apical transport. only one d-glucose carrier was found in vesicles from each intestinal segment. in both cases, the d-glucose transport system was sodium-dependent, phlo ... | 2004 | 15630546 |
| calcium handling in sparus auratus: effects of water and dietary calcium levels on mineral composition, cortisol and pthrp levels. | juvenile gilthead sea bream (sparus auratus l.; 10-40 g body mass) were acclimatized in the laboratory to full strength (34 per thousand) or dilute (2.5 per thousand) seawater and fed normal, calcium-sufficient or calcium-deficient diet for nine weeks. mean growth rate, whole-body calcium and phosphorus content and accumulation rates were determined, as well as plasma levels of ionic and total calcium, cortisol and parathyroid hormone related protein (pthrp; a hypercalcemic hormone in fish). whe ... | 2004 | 15498953 |
| stimulation of cortisol release by the n terminus of teleost parathyroid hormone-related protein in interrenal cells in vitro. | the mode of action of pthrp in the regulation of sea bream (sparus auratus) interrenal cortisol production was studied in vitro using a dynamic superfusion system. piscine (1-34)pthrp (10(-6)-10(-11) m) stimulated cortisol production in a dose-dependent manner. the ed50 of (1-34)pthrp was 2.8 times higher than that of (1-39)acth, and maximum increase in cortisol production in response to 10(-8) m of (1-34)pthrp was approximately 7-fold lower than for 10(-8) m of (1-39)acth. in contrast to (1-34) ... | 2005 | 15459121 |
| osmoregulatory action of 17beta-estradiol in the gilthead sea bream sparus auratus. | the osmoregulatory action of 17beta-estradiol (e2) was examined in the euryhaline teleost sparus auratas. in a first set of experiments, fish were injected once with vegetable oil containing e2 (1, 2 and 5 microg/g body weight), transferred 12h after injection from sea water (sw, 38 ppt salinity) to hypersaline water (hsw, 55 ppt) or to brackish water (bw, 5 ppt salinity) and sampled 12h later (i.e. 24 h post-injection). in a second experiment, fish were injected intraperitoneally with coconut o ... | 2004 | 15449347 |
| nutritional assessment of somatolactin function in gilthead sea bream (sparus aurata): concurrent changes in somatotropic axis and pancreatic hormones. | the role of somatolactin (sl) in the regulation of energy homeostasis in gilthead sea bream (sparus aurata) has been analysed. first, a down-regulation of plasma sl levels in response to gross shifts in dietary amino acid profile and the graded replacement of fish meal by plant protein sources (50%, 75% and 100%) has been observed. thus, the impaired growth performance with changes in dietary amino acid profile and dietary protein source was accompanied by a decrease in plasma sl levels, which a ... | 2004 | 15369843 |
| histological alterations in the liver of sea bream, sparus aurata l., caused by short- or long-term feeding with vegetable oils. recovery of normal morphology after feeding fish oil as the sole lipid source. | this study evaluated the effects of fish oil (fo) replacement by vegetable oils [soybean oil (so), rapeseed oil (ro), linseed oil (lo)] and subsequent feeding with fo on the liver morphology of sea bream. a short-term trial (3 months) and long-term trial (6 months) were carried out feeding sea bream with the following experimental diets: fo100%; so60% + fo40%; ro60% +fo40%; lo60% + fo40%; so + ro +lo60% + fo40%. finally, all groups from the long-term trial were fed with fo100% for 95 days (washo ... | 2004 | 15357712 |
| adaptation of lipid metabolism, tissue composition and flesh quality in gilthead sea bream (sparus aurata) to the replacement of dietary fish oil by linseed and soyabean oils. | linseed (lo) and soyabean (so) oils were evaluated as fish-oil (fo) substitutes in the diets of marketable-sized gilthead sea bream (sparus aurata). practical diets were designed factorially with the lipid added as follows (%): fo 100, lo 60+fo 40, lo 80+fo 20, so 60+fo 40, so 80+fo 20. the effects of experimental diets on growth, fatty acids patterns in liver and muscle, flesh quality variables and activities of selected enzymes involved in lipid synthesis and catabolism were determined at the ... | 2004 | 15230986 |
| molecular cloning of hepatic glucose-6-phosphatase catalytic subunit from gilthead sea bream (sparus aurata): response of its mrna levels and glucokinase expression to refeeding and diet composition. | to examine the relationship between structure and function of glucose-6-phosphatase (g6pase) in fish, we undertook molecular cloning and modulation of g6pase expression by starvation and refeeding on diets with different nutrient composition in the liver of the carnivorous fish, sparus aurata. a cdna encoding the full-length g6pase catalytic subunit from the liver of s. aurata was isolated. this cdna encodes a 350-amino acid protein, with low homology to the mammalian g6pase, although it contain ... | 2004 | 15193269 |
| the n-terminal sequence directs import of mitochondrial alanine aminotransferase into mitochondria. | herein, we report cloning and subcellular localization of two alanine aminotransferase (alt) isozymes, calt and malt, from liver of gilthead sea bream (sparus aurata). cho cells transfected with constructs expressing calt or malt as c- or n-terminal fusion with the enhanced green fluorescent protein (egfp) showed that calt is cytosolic, whereas malt localized to mitochondria. fusion of egfp to malt n-terminus or removal of amino acids 1-83 of malt avoided import into mitochondria, supporting evi ... | 2004 | 15147904 |
| glomerulonephritis and immunosuppression associated with dietary essential fatty acid deficiency in gilthead sea bream, sparus aurata l., juveniles. | an experiment was conducted to determine the effect of an essential fatty acid (efa) deficient diet on growth, immune status and renal morphology of juvenile gilthead sea bream, using two diets: a control diet containing 2% (dw) of n-3 high unsaturated fatty acid (n-3 hufa) and a diet formulated to be deficient in efa for this species and containing 0.5% dw of n-3 hufa (diet nfa). after 9 weeks of feeding the efa-deficient diet fish showed a reduction in growth compared with the control group (1 ... | 2004 | 15139908 |
| isolation of a novel aquaglyceroporin from a marine teleost (sparus auratus): function and tissue distribution. | the aquaporins (formerly called the major intrinsic protein family) are transmembrane channel proteins. the family includes the chip group, which are functionally characterised as water channels and the glp group, which are specialised for glycerol transport. the present study reports the identification and characterisation of a novel glp family member in a teleost fish, the sea bream sparus auratus. a sea bream aquaporin (sbaqp) cdna of 1047 bp and encoding a protein of 298 amino acids was isol ... | 2004 | 14978062 |
| development of two bone-derived cell lines from the marine teleost sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix gla protein and osteocalcin. | a growing interest in the understanding of the ontogeny and mineralization of fish skeleton has emerged from the recent implementation of fish as a vertebrate model, particularly for skeletal development. whereas several in vivo studies dealing with the regulation of bone formation in fish have been published, in vitro studies have been hampered because of a complete lack of fish-bone-derived cell systems. we describe here the development and the characterization of two new cell lines, designate ... | 2004 | 14762709 |
| life cycle of ceratothoa oestroides, a cymothoid isopod parasite from sea bass dicentrarchus labrax and sea bream sparus aurata. | ceratothoa oestroides (risso, 1826) (isopoda: cymothoida) is a protandric hermaphrodite parasite on a wide range of wild fish species. in recent years it has become a threat to cage-reared fish facilities, where high fish density provides optimal conditions for transmission. its impact on fish health and economical gain is significant, varying from growth retardation and decreased immunocompetency to direct loss due to mass mortalities of juvenile fishes. because of the sheltered location of the ... | 2003 | 14735926 |
| cloning and analysis of expression of a gilthead sea bream (sparus aurata) mx cdna. | in the current work, we have cloned and sequenced the full cdna for a mx protein in the gilthead sea bream (sparus aurata) by race pcr. the mx cdna of 2182 bp contained an open reading frame of 1857 bp that codes for a protein of 618 aa. within the coding sequence, characteristic features of mx proteins were found, such as a tripartite guanosine-5'-triphosphate (gtp)-binding motif (gxxxsgks/t, dxxg and t/nkxd), the signature of the dynamin family, lprg(s/k)givtr, and a sequence that codes for a ... | 2004 | 14675830 |
| dna damage induced by copper on erythrocytes of gilthead sea bream sparus aurata and mollusk scapharca inaequivalvis. | heavy metal pollution in rivers and its impact on aquatic ecosystems is a dynamic process. fish are ideal indicators of heavy metal contamination in aquatic systems because they occupy different trophic levels and are of different sizes and ages. in particular, copper is an essential trace metal for living organisms and it is present in all natural waters and sediments. in this paper, we report data on the effect of copper on dna erythrocytes from the teleost gilthead sea bream sparus aurata and ... | 2003 | 14674588 |
| hormonal regulation of vasa-like messenger rna expression in the ovary of the marine teleost sparus aurata. | the vasa gene is an important maternal regulator of primordial germ cell (pgc) development in both vertebrate and invertebrate models. it is also expressed in the mature gonads, but its role in these tissues is still unclear. in oviparous species, oogenesis is a complex process under hormonal control: estrogens, gonadotropins, and other hormones operate at different stages of oogenesis, regulating meiosis, vitellogenesis, follicle maturation, and egg release. the aim of this work is the determin ... | 2004 | 14613903 |
| annual variation of complement, lysozyme and haemagglutinin levels in serum of the gilthead sea bream sparus aurata. | | 2003 | 14550674 |
| biochemical changes during post-larval growth in white muscle of gilthead sea bream (sparus aurata) fed zinc-fortified diets. | | 2003 | 14535393 |
| apoptosis in sea bream sparus aurata eggs. | in the present study, the sea-bream sparus aurata, a pelagic egg spawner, was used as experimental model, in order to establish the occurrence of apoptosis in vertebrates with external reproduction. the same female ovulates floating and nonfloating eggs, but only the former, after fertilization, proceed to embryo development. the eggs were divided into floating and nonfloating and both were analyzed for the presence of several apoptosis markers. the results here reported provide evidence that th ... | 2003 | 14502608 |
| isolation of sparus auratus prolactin gene and activity of the cis-acting regulatory elements. | a sea bream prolactin (sbprl) gene was isolated using a prolactin cdna fragment, generated by pcr as a probe. the gene analyzed comprises 3.5 kb of dna containing five exons as described previously for other fish prl genes. analysis of 1.0 kb of the proximal promoter sequence reveals a consensus tataa box, up to seven (a/t)3ncat consensus motifs for binding of the pituitary-specific factor pit-1 and putative creb and gata binding sites. cho culture cells co-transfected with a sbprl promoter sequ ... | 2003 | 13129503 |
| expression and cellular localization of insulin-like growth factor-ii protein and mrna in sparus aurata during development. | the spatial localization of igf-ii protein and mrna was investigated during larval and postlarval developmental stages of the gilthead sea bream (sparus aurata) by immunohistochemistry and in situ hybridization, using specific antisera and riboprobes. steady-state levels of igf-ii mrna in larvae were determined by northern blot analysis and were found to be increased. immunoreactivity towards igf-ii was found in larval skin, muscle, gills, gut, olfactory epithelium and kidney. after metamorphosi ... | 2003 | 12904176 |
| growth hormone expression in ontogenic development in gilthead sea bream. | the pattern of expression of the growth hormone (gh) gene was studied during the early development of gilthead sea bream ( sparus aurata). the gh transcript was detected from the 2nd day of the larval stage onwards. in the next stages the expression level fluctuated, possibly due to different regulatory factors. the distribution of gh mrna studied by in situ hybridization (ish) was found to be pituitary specific. hybridization signals for gh mrna were detected for the first time in 4-day-old lar ... | 2003 | 12827495 |
| acclimation of s aurata to various salinities alters energy metabolism of osmoregulatory and nonosmoregulatory organs. | the impact of different environmental salinities on the energy metabolism of gills, kidney, liver, and brain was assessed in gilthead sea bream (sparus aurata) acclimated to brackish water [bw, 12 parts/thousand (ppt)], seawater (sw, 38 ppt) and hyper saline water (hsw, 55 ppt) for 14 days. plasma osmolality and levels of sodium and chloride presented a clear direct relationship with environmental salinities. a general activation of energy metabolism was observed under different osmotic conditio ... | 2003 | 12816745 |
| developmental ontogeny of prolactin and prolactin receptor in the sea bream (sparus aurata). | the expression of prl and its receptor (prlr) were characterised during sea bream embryonic and larval development, by semi-quantitative and quantitative rt-pcr, respectively, until 46 days post-hatch (dph). immunocytochemistry with antisera specific for sea bream prlr was carried out with larval sections from hatching up to 46 dph. a single transcript of prl (1.35 kb) and prlr (2.8 kb) identical to the transcripts previously characterised in adult tissue, are present in sea bream embryos and la ... | 2003 | 12812779 |
| differential expression and cellular localization of somatolactin-1 and -2 during early development in the gilthead sea bream. | the patterns of expression of the somatolactin 1 and 2 (sl1 and sl2) transcripts were studied during the early development of the gilthead sea bream (sparus aurata). gene expression of sl1 and sl2 were detected in embryos and in larvae, although both transcripts presented different levels of expression. the sl1 transcripts in contrast to the sl2 transcripts presented high expression levels in embryos and younger larvae. moreover, the sl2 transcripts were slightly present or absence in embryonic ... | 2003 | 12765646 |
| molecular characterisation of sea bream (sparus aurata) transforming growth factor beta1. | a transforming growth factor beta1 (tgf beta1) full length cdna was characterised and sequenced from the head kidney of sea bream (sparus aurata) previously challenged with a nodavirus. the cloned cdna of 1778bp contains a predicted open reading frame of 379 amino acids, which includes the mature peptide region of 112 amino acids. the regulating region of the peptide possesses four potential n-linked glycosylation sites (n-x-t/s), as well as an rgd integrin binding site, an rkkr tetrabasic cut s ... | 2003 | 12711274 |
| the regulatory action of estrogen and vasoactive intestinal peptide on prolactin secretion in sea bream (sparus aurata, l.). | the effect of estradiol-17beta (e(2)) implants on the in vitro secretion of prolactin (prl) and its modulation by vasoactive intestinal peptide (vip) in a marine teleost, sea bream (sparus aurata l.), was determined. experiments were conducted during winter and spring. during winter, fish (n=130, body weight 50-70 g) were randomly divided into 2 groups; control and e(2) treated (10 mg/kg, wet weight). fish were sacrificed after 7 days treatment and in vitro pituitary cultures in ringer bicarbona ... | 2003 | 12679088 |
| myostatin precursor is present in several tissues in teleost fish: a comparative immunolocalization study. | in this study, the distribution of myostatin was investigated during larval and postlarval developmental stages of sparus aurata(sea bream), solea solea(sole) and brachydanio rerio(zebrafish) by immunohistochemistry using antisera raised against a synthetic peptide located within the precursor region of sea bream myostatin. all the three species examined showed the strongest immunoreactivity in red skeletal muscle in juveniles and adults. during larval development of sea bream, strong staining w ... | 2003 | 12596043 |
| transient expression pattern of prolactin in sparus aurata. | using reverse transcription-polymerase chain reaction and in situ hybridization, the expression of the prolactin (prl) gene was determined during development in gilthead sea bream (sparus aurata) for the first time. the mrna for prl was detected from the second day of the larval stage onwards. this transcript was also located in the adenohypophysial cells, starting at four days post-hatching and was found to be pituitary-specific. moreover, the possible involvement of prl in asynchronous growth ... | 2003 | 12580762 |
| immunocytochemical and ultrastructural characterization of mammosomatotrope-, growth hormone-, and prolactin-cells from the gilthead sea bream (sparus aurata l., teleostei): an ontogenic study. | growth hormone (gh), prolactin (prl), and mammosomatotrope (ms) cells of gilthead sea bream, sparus aurata, a teleost fish, were studied in specimens from hatching to 15 months (adults) using conventional electron microscopy and an immunogold method using anti-tilapia gh sera and anti-chum salmon prl serum. ms cells, immunoreactive to both anti-gh sera and anti-prl sera, had been first identified in fish in a previous study in newly hatched larvae and in older larvae and juvenile specimens of sp ... | 2003 | 12520552 |
| nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (sparus aurata). | to examine the role of glucose-6-phosphatase (g6pase) in glucose homeostasis in the diabetes-like experimental model of carnivorous fish, we analysed postprandial variations and the effect of starvation, ration size and diet composition on the regulation of g6pase expression at the enzyme activity and mrna level in the liver of gilthead sea bream (sparus aurata). g6pase expression increased in long-term starved or energy-restricted fish. in contrast to data reported for other fish species, short ... | 2002 | 12493082 |
| endocrine mediators of seasonal growth in gilthead sea bream (sparus aurata): the growth hormone and somatolactin paradigm. | regulation of somatolactin (sl) and the somatotropic axis was examined year-around at three different stocking times (spring, summer, and autumn) in a mediterranean fish, the gilthead sea bream (sparus aurata). the overall timing of plasma growth hormone (gh) increase was similar among trials (late spring-early summer), but the range of variation year-around was different and followed changes in food intake. total plasma insulin-like growth factor-i primarily followed changes on growth rates, an ... | 2002 | 12392683 |
| co-expression of somatolactin mrnas in the pituitary gland of gilthead sea bream. | the expression of mrnas of the two types of somatolactin which have been found, up to the present, in the pituitary of gilthead sea bream (sparus aurata) have been analyzed by in situ hybridization (ish). the method of non-radioactive ish, which we optimize in this study, uses oligonucleotides labelled in the 5' end with biotin or dioxygenin as probes. this allows simple and double ish of high specificity and sensitivity to be performed. the distinct somatolactin oligoprobes used present a stron ... | 2002 | 12382144 |
| hormonal mechanisms regulating hepatic vitellogenin synthesis in the gilthead sea bream, sparus aurata. | experiments were carried out to study in vitro the effects of 17beta-estradiol (e(2)), homologous pituitary homogenate (hph), and recombinant red sea bream growth hormone (sbgh) on vitellogenin (vtg) secretion from cultured sea bream liver fragments. basal secretion of vtg was found to be significantly higher in the prespawning period, compared with sea bream liver in the spawning and postspawning periods. similarly, the sea bream liver obtained during the prespawning period responded more signi ... | 2002 | 12176724 |
| calcium balance in sea bream (sparus aurata): the effect of oestradiol-17beta. | in all teleost fishes vitellogenesis is triggered and maintained by oestradiol-17beta (e2) and is accompanied by an increase of blood plasma calcium and phosphate. the action of this hormone on calcium metabolism was investigated by treating fast-growing immature juvenile sea bream (sparus aurata) with coconut butter implants alone (control) or implants containing 10 microg/g e2. treatment with e2 induced the production of circulating vitellogenin, a 2.5-fold increase in plasma ionic ca2+ and a ... | 2002 | 12010645 |
| comparative quality assessment of cultured and wild sea bream (sparus aurata) stored in ice. | comparative quality assessment of cultured and wild sea bream stored in ice for up to 23 days was achieved by the monitoring of sensory quality, levels of nucleotide, nucleotide breakdown products, and texture by a texturometer. the changes in sensory quality of both raw and cooked fish were assessed using the modified tasmanian and torry schemes, respectively. k and related values (freshness indicators), namely, k, k(i), g, p, h, and f(r), were calculated. linear increases (r(2) > or = 0.99) in ... | 2002 | 11902954 |
| leptotheca sparidarum n. sp. (myxosporea: bivalvulida), a parasite from cultured common dentex (dentex dentex l.) and gilthead sea bream (sparus aurata l.) (teleostei: sparidae). | a new myxosporean, leptotheca sparidarum n. sp., is described from the trunk kidney of two sparid fish, sparus aurata and dentex dentex, in several culture facilities from the western mediterranean coasts. it is distinguished from all the previously described species by spore morphometrics. spores with two equal polar capsules and one binucleated sporoplasm. spore measurements from d. dentex were 5-7.1 micron long x 8.8-12.3 micron thick x 5.88-6.18 micron wide; polar capsules 2.6-3.5 micron in ... | 2009 | 11831771 |
| cyclic adpr and calcium signaling in sea bream (sparus aurata) egg fertilization. | the cell egg is in a state of quiescence and only after its fusion with the sperm, a series of pre-programmed metabolic processes will be activated, culminating with embryonic development. the egg/sperm fusion induces a transitory increase of ca(2+) in the cytoplasm, which is responsible for the activation of both precocious and late reactions. the release of ca(2+) occurs by stimulation of the ionic specific channels. in addition to ip(3), a new ca-release inducer was recently evidenced, cyclic ... | 2002 | 11803557 |
| gonadal steroidogenesis in response to estradiol-17beta administration in the sea bream (sparus aurata l.). | the sea bream (sparus aurata) is a protandrous hermaphrodite teleost fish in which estrogen administration induces testicular regression without influencing ovarian development. to analyze the changes in steroidogenesis of fish treated with two levels of estrogen (2 and 10 mg. kg(-1)) and untreated control fish, fragments of gonads were incubated with tritiated 17-hydroxyprogesterone and the metabolites identified. the ability to extract radioactivity decreased with incubation time and was lower ... | 2001 | 11703074 |
| parathyroid hormone-related protein: a calcium regulatory factor in sea bream (sparus aurata l.) larvae. | the effects of an n-terminal peptide (amino acids 1-38) of fugu parathyroid hormone-related protein (pthrp 1-38) on calcium regulation of larval sea bream were investigated in seawater (36 per thousand) and after transfer to dilute seawater (12 per thousand). exposure to pthrp 1-38 evoked a 1.5-fold increase in calcium influx in both full-strength and dilute seawater. calcium influx in dilute seawater-adapted larvae was roughly one-half that observed in full-strength seawater controls. pthrp 1-3 ... | 2001 | 11507001 |
| molecular cloning and sequence of gilthead sea bream (sparus aurata) alpha-skeletal actin: tissue and developmental expression. | in the present study, the sparus aurata alpha-skeletal actin was cloned from a mixed larvae complementary dna library. the clone isolated was 1523 bp long with an open reading frame of 1134 bp coding for a 377-amino acid protein. the deduced amino acid sequence of sea bream alpha-actin is identical to fugu alpha-actin-1. the expression of alpha-actin was initiated at the onset of segmentation. in adult fish, alpha-actin is expressed predominantly in white and red muscle. | 2001 | 11470440 |
| immunocytochemical and ultrastructural characterization of somatolactin cells from the gilthead sea bream (sparus aurata l., teleostei): an ontogenic study (from newly hatched to adults). | for the first time, somatolactin (sl) cells have been ultrastructurally identified and characterized during the ontogeny of gilthead sea bream, sparus aurata, using specimens ranging in age from hatching to 15 months. the sl cells were identified by an immunogold method using anti-cod sl serum. the sl-immunoreactivity was mostly located on the secretory granules of the cells, although some vesicles of variable size and shape with a medium electron-dense content, and some irregular secretory gran ... | 2001 | 11453162 |
| proliferation zones in the adult brain of a sequential hermaphrodite teleost species (sparus aurata). | teleost sex change is an important model to understand general principles of sexual differentiation and plasticity in the adult brain. the present study is the first to examine the proliferation zones in the adult brain of males, females and sex-changing individuals of a protandrous teleost species (sparus aurata), by means of 5-bromo-2-deoxyuridine immunocytochemistry. postnatal neurogenesis in the marine teleost brain was found in ventricular and subventricular areas of the brain that in most ... | 2000 | 11326136 |
| cultured fish cells metabolize octadecapentaenoic acid (all-cis delta3,6,9,12,15-18:5) to octadecatetraenoic acid (all-cis delta6,9,12,15-18:4) via its 2-trans intermediate (trans delta2, all-cis delta6,9,12,15-18:5). | octadecapentaenoic acid (all-cis delta3,6,9,12,15-18:5; 18:5n-3) is an unusual fatty acid found in marine dinophytes, haptophytes, and prasinophytes. it is not present at higher trophic levels in the marine food web, but its metabolism by animals ingesting algae is unknown. here we studied the metabolism of 18:5n-3 in cell lines derived from turbot (scophthalmus maximus), gilthead sea bream (sparus aurata), and atlantic salmon (salmo salar). cells were incubated in the presence of approximately ... | 2001 | 11269695 |
| pituitary and interrenal function in gilthead sea bream (sparus aurata l., teleostei) after handling and confinement stress. | dynamics of adrenocorticotropin (acth), alpha melanocyte-stimulating hormone (alpha-msh), n-acetylated-beta-endorphin (n-ac-beta-end), cortisol, and growth hormone (gh) were investigated in gilthead sea bream (sparus aurata) stressed by handling plus confinement. as indices of the secondary stress response, plasma levels of glucose, lactate, and plasma ions were monitored. within 1 h, plasma cortisol and acth levels increased above the control values but gh levels decreased. subsequently, at 24 ... | 2001 | 11254375 |
| histopathology of cultured sea bream sparus aurata infected with sanguinicolid trematodes. | the present study is the first report of a sanguinicolid infection affecting sea bream sparus aurata cultured in net cages in the ne of spain. the disease was associated with trickling mortalities during the cold season (1999 and 2000). examination of gill wet mounts of the affected population revealed that sanguinicolid infection was present in 82.6 and 100% of the fish sampled in 1999 and 2000, respectively. adult flukes, which were located in the kidney, were tentatively identified as members ... | 2001 | 11253874 |
| molecular components related to egg viability in the gilthead sea bream, sparus aurata. | in pelagic egg spawners, the production of large numbers of sinking eggs, unable to develop into embryos, represents one of the major limiting factors in controlled reproduction. the aim of this study is to elucidate the molecular differences between floating and nonfloating eggs at cytoplasmic and nuclear level. comparison of analyses between floating and nonfloating sea bream sparus aurata eggs evidenced differences in vitelline envelope protein components, such differences being probably rela ... | 2001 | 11170274 |
| cloning, characterization, and tissue distribution of prolactin receptor in the sea bream (sparus aurata). | the prolactin receptor (prlr) was cloned and its tissue distribution characterized in adults of the protandrous hermaphrodite marine teleost, the sea bream (sparus aurata). an homologous cdna probe for sea bream prlr (sbprlr) was obtained by rt-pcr using gill mrna. this probe was used to screen intestine and kidney cdna libraries from which two overlapping clones (1100 and 2425 bp, respectively) were obtained. these clones had 100% sequence identity in the overlapping region (893 bp) and were us ... | 2001 | 11161768 |
| identification of mammosomatotropes, growth hormone cells and prolactin cells in the pituitary gland of the gilthead sea bream (sparus aurata l., teleostei) using light immunocytochemical methods: an ontogenetic study. | growth hormone (gh) and prolactin (prl) immunoreactivities in the adenohypophysis of sparus aurata specimens from newly hatched until 48-months-old were detected using the peroxidase-antiperoxidase method. gh cells and prl cells, and cells that were immunoreactive to both gh and prl antisera, called mammosomatotropes (ms cells), were found. this is the first report on the identification of ms cells in fish, which were found in newly hatched and older larvae and juvenile specimens. gh and prl cel ... | 2000 | 11089933 |
| pituitary proopiomelanocortin-derived peptides and hypothalamus-pituitary-interrenal axis activity in gilthead sea bream (sparus aurata) during prolonged crowding stress: differential regulation of adrenocorticotropin hormone and alpha-melanocyte-stimulating hormone release by corticotropin-releasing hormone and thyrotropin-releasing hormone. | plasma levels of cortisol, growth hormone (gh), adrenocorticotropin hormone (acth), alpha-melanocyte-stimulating hormone (alpha-msh), n-acetyl-beta-endorphin, in vitro acth-stimulated cortisol secretion, and in vitro corticotropin-releasing hormone (crh)- and thyrotropin-releasing hormone (trh)-stimulated acth and alpha-msh secretion were investigated in gilthead sea bream exposed to high stocking density (30 kg m(-3)) for 23 days. within 3 days after the onset of crowding, plasma levels of cort ... | 2000 | 10936035 |
| alpha-msh acetylation in the pituitary gland of the sea bream (sparus aurata l.) in response to different backgrounds, confinement and air exposure. | msh is a pituitary hormone derived by post-translational processing from pomc and involved in stress and background adaptation. n-terminal acetylation of msh to monoacetyl alpha-msh or diacetyl alpha-msh increases the bioactivity of the peptide. the aim of this study was to characterize alpha-msh acetylation in the sea bream (sparus aurata l.) pituitary gland in response to the stressors air exposure and confinement, as well as in fish adapted for 15 days to a white, gray or black background. pi ... | 2000 | 10927632 |
| cloning and expression of an elongation factor-1alpha in sea bream (sparus aurata) larvae and adult tissue. | a clone encoding the polypeptide elongation factor ef-1alpha was isolated from a complementary dna library prepared from sea bream (spartus aurata) larvae 1 to 10 days after hatching. the deduced amino acid sequence is between 82% and 95% similar to ef-1alpha in other animal species. ef-1alpha messenger rna is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. this increase presumably re ... | 2000 | 10811957 |
| 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene expression is regulated by diet composition and ration size in liver of gilthead sea bream, sparus aurata. | modulation of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6pf-2-k/fru-2,6-p(2)ase) gene expression by diet composition and ration size was studied in the liver of gilthead sea bream, sparus aurata. from five different types of diet supplied to fish, those with either high carbohydrate/low protein or high carbohydrate/low lipid content stimulated 6pf-2-k/fru-2,6-p(2)ase expression at the levels of mrna, immunodetectable protein and kinase activity as well as promoting higher fructose-2, ... | 2000 | 10760583 |
| effect of diet composition and ration size on key enzyme activities of glycolysis-gluconeogenesis, the pentose phosphate pathway and amino acid metabolism in liver of gilthead sea bream (sparus aurata). | the effects of diet composition and ration size on the activities of key enzymes involved in intermediary metabolism were studied in the liver of gilthead sea bream (sparus aurata). high-carbohydrate, low-protein diets stimulated 6-phosphofructo 1-kinase (ec 2.7.1.11), pyruvate kinase (ec 2.7.1.40), glucose-6-phosphate dehydrogenase (ec 1.1.1.49) and 6-phosphogluconate dehydrogenase (ec 1.1.1.44) enzyme activities, while they decreased alanine aminotransferase (ec 2.6.1.2) activity. a high degre ... | 1999 | 10655969 |
| unilateral eye abnormalities in reared mediterranean gilthead sea bream. | eye abnormalities in reared gilthead sea bream (sparus aurata) were investigated clinically and by histological techniques. a significant number of fish had a cataract in one eye and ocular inflammation including gross exophthalmos. in a small number of fish gas bubbles were observed in the anterior chamber of the eye. histological examination provided no evidence of an infectious process and a possible behavioural aetiology is discussed. | 1999 | 10596872 |
| the effect of estrogen on the gonads and on in vitro conversion of androstenedione to testosterone, 11-ketotestosterone, and estradiol-17beta in sparus aurata (teleostei, sparidae). | the effects of estrogen on gonad morphology and steroidogenesis of sea bream, sparus aurata, a protandrous hermaphrodite teleost, were investigated. fish were treated in winter/spring for different periods with 17alpha-ethynylestradiol (ethe(2); experiment 1) and in summer with two doses of estradiol-17beta (e(2); experiment 2). estrogen was more effective in summer. its main effect on the gonad was inhibition of testicular growth and of male germ cell development beyond the spermatogonia stage, ... | 1999 | 10525362 |
| the stress response of the gilthead sea bream (sparus aurata l.) to air exposure and confinement. | we investigated short-term effects (up to 24 h) of air exposure and confinement, and long-term effects (up to 11 days) of confinement, to elucidate signalling pathways in the stress response of gilthead sea bream sparus aurata l. plasma glucose and lactate were taken as indicators of sympathetic activation, and alpha-melanocyte stimulating hormone (alpha-msh), adrenocorticotrophic hormone (acth) and cortisol as indicators of activation of the brain-pituitary-interrenal (bpi) axis. air exposure f ... | 1999 | 10495417 |
| seasonal changes of zinc, copper, and iron in gilthead sea bream (sparus aurata) fed fortified diets. | four groups of gilthead sea bream (sparus aurata) were fed diets with additional metal contents: a basal diet (diet a) contained zn at 60.9 +/- 1.9 mg/kg diet, cu at 3.9 +/- 0.9 mg/kg diet, and fe at 138.3 +/- 6.8 mg/kg diet; the other diets were supplemented with copper (20 mg/kg, diet b), iron (100 mg/kg, diet c), or zinc (300 mg/kg, diet d). two consecutive year-classes (0+ and 1+ age fish) from the same parent stock were examined. several fish tissues were analyzed for metal contents in five ... | 1999 | 10433345 |
| biogenic amines and sensory changes associated with the microbial flora of mediterranean gilt-head sea bream (sparus aurata) stored aerobically at 0, 8, and 15 degrees c. | changes in the concentrations of tyramine, agmatine, putrescine, cadaverine, spermidine, tryptamine, spermine, histamine, and trimethylamine were studied in parallel with the development of the microbial population during the storage of mediterranean gilt-head sea bream (sparus aurata) at three temperatures (0, 8, 15 degrees c). changes in sensory scores were also recorded. pseudomonads and h2s-producing bacteria were the dominant microorganisms. enterobacteriaceae and lactic acid bacteria were ... | 1999 | 10419215 |
| fatty acid metabolism in marine fish: low activity of fatty acyl delta5 desaturation in gilthead sea bream (sparus aurata) cells. | marine fish have an absolute dietary requirement for c20 and c22 highly unsaturated fatty acids. previous studies using cultured cell lines indicated that underlying this requirement in marine fish was either a deficiency in fatty acyl delta5 desaturase or c18-20 elongase activity. recent research in turbot cells found low c18-20 elongase but high delta5 desaturase activity. in the present study, the fatty acid desaturase/elongase pathway was investigated in a cell line (saf-1) from another carn ... | 1999 | 10380114 |
| cloning and sequencing of the gilthead sea bream estrogen receptor cdna. | we report here the complete nucleotide sequence of a cdna clone containing the full-coding sequence of the sparus aurata estrogen receptor (er) isolated from an expression library prepared from gilthead sea bream liver poly a+ rna. the library was screened using a single strand rainbow trout er cdna probe, corresponding to the c-d domain. the cdna sequence containing an insert of 2369 nucleotides was found to encode a protein of 579 amino acids. the 5'- and 3'-untranslated regions of the message ... | 1999 | 10376207 |
| dietary chromic oxide does not affect the utilization of organic compounds but can alter the utilization of mineral salts in gilthead sea bream sparus aurata. | this study was conducted to determine whether the level of chromic oxide supplemented to diets containing gelatinized starch as the carbohydrate source affects digestibility, body composition, growth performances, and liver enzyme activities in gilthead sea bream, sparus aurata. gilthead sea bream fingerlings were fed diets containing gelatinized corn starch as the carbohydrate source and several levels of chromic oxide (0, 5, 10 and 20 g/kg) for 6 wk. no effect of dietary chromium level was det ... | 1999 | 10222399 |
| red muscle development of gilthead sea bream sparus aurata (l.): structural and ultrastructural morphometry. | the transverse red and white muscle area, the superficial red muscle fibres area and their percentages of mitochondria (%mit), myofibrils (%myof) and sarcoplasm (%sarc) were determined in the mediterranean teleost gilthead sea bream, sparus aurata (l.). fish aged from hatching to 78 days were studied. the proportional growth of the red and white muscles was higher for the red muscle in the first half of the larval stage (1-35 days). then the opposite relationship was observed. the hypertrophic g ... | 1999 | 10208029 |
| glomerular disease associated with polysporoplasma sparis (myxozoa) infections in cultured gilthead sea bream, sparus aurata l. (pisces: teleostei). | polysporoplasma sparis infection was studied in gilthead sea bream from different mariculture systems of the spanish coasts. culture conditions influenced the infection dynamics, as the parasite appeared only in semi-intensive cultures and was not found in intensive closed systems nor in open ones. no clear seasonal pattern was observed. no fish weighing less than 51 g was found parasitized in any group. a statistically significant dependence between infection prevalence and host weight was obse ... | 1999 | 10205800 |
| cloning, expression, and tissue localisation of prolactin in adult sea bream (sparus aurata). | a major action of prolactin (prl) in teleost fish is the maintenance of hydromineral balance in euryhaline species in fresh water. the function of prl in marine teleosts is less certain and unlike euryhaline teleosts, such as tilapia and salmon, there is relatively little information about protein or gene structure. associated with studies to determine potential functions of prl, pituitary prolactin cdna has been cloned and sequenced from sea bream (sparus aurata), a marine teleost. the sequence ... | 1999 | 10094859 |
| negative covariance suggests mutation bias in a two-locus microsatellite system in the fish sparus aurata. | constraints on microsatellite length appear to vary in a species-specific manner. we know very little about the nature of these constraints and why they should vary among species. while surveying microsatellite variation in the mediterranean gilthead sea bream, sparus aurata, we discovered an unusual pattern of covariation between two closely linked microsatellite loci. one- and two-locus haplotypes were scored from pcr amplification products of each locus separately and both loci together. in a ... | 1998 | 9832532 |
| muscle development in gilthead sea bream (sparus aurata, l.) and sea bass (dicentrarchus labrax, l.): further histochemical and ultrastructural aspects. | the histochemical profiles--matpase and nadh-tr reactions--of the red and white muscle fibres of gilthead sea bream and sea bass were determined from the first week after hatching. modifications of the matpase technique by combinations of ph/time/molarity were carried out in order to compare the sensitivity of the myosin atpase of each muscle fibre type of the lateral muscle. results showed that the staining of muscle fibres was independent of small modifications in the technique. the intermedia ... | 1998 | 9741144 |
| acetyl salmon endorphin-like and interrenal stress response in male gilthead sea bream, sparus aurata. | the present study investigates the role of melanotrope proopiomelanocortin-derived peptide in the interrenal stress response to different stressors in male gilthead sea bream, sparus aurata. plasma cortisol and acetyl salmon endorphin (acetyl s-ep), as well as pituitary acetyl s-ep contents, were measured during two stress paradigms: (a) long-term (1-month) confinement and crowding, and (b) short-term (60-min) confinement, crowding, and manipulation. in addition, naltrexone, a highly specific op ... | 1998 | 9705580 |
| distribution of vasoactive intestinal peptide in the brain and hypothalamo-hypophysial system of the sea bream (sparus aurata). | | 1998 | 9629174 |
| insulin during the early postembryonic development of the gilt-head sea bream, sparus aurata: ultrastructural, immunohistochemical, and biochemical studies. | insulin was sought during the early postembryonic development of gilt-head sea bream, sparus aurata, using ultrastructural, immunohistochemical and biochemical techniques. the endocrine pancreas appeared at hatching (day 0) as a single cluster of morphologically similar cells. secretory granules formed from day 1 onwards but the cells could only be identified as insulin-producing b cells at the end of the endotrophic period (day 3-day 4). insulin-immunoreactive cells were detected in the pancrea ... | 1998 | 9570935 |
| the post-larval development of lateral musculature in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.). | fibre-type differentiation of lateral musculature has been studied in gilthead sea bream sparus aurata (l.) and sea bass dicentrarchus labrax (l.) during post-larval development using ultrastructural, histochemical and morphometric techniques. the study showed three muscle layers: red, intermediate (or pink) and white. initially, most of the red muscle showed low myosin atpase (m-atpase) activity fibres, whereas near the transverse septum some small high m-atpase activity fibres appeared and lat ... | 1998 | 9505442 |
| ontogeny of immunoreactive somatolactin cells in the pituitary of gilthead sea bream (sparus aurata l., teleostei). | this is the first report on the identification of somatolactin (sl) cells during the early developmental stages of the teleost fish sparus aurata. the sl cells were followed from newly hatched until 46 months. sl cells were immunocytochemically identified at light microscopical level with anti-cod sl in the developing pituitary using the peroxidase-antiperoxidase method. sl cells first appeared in newly hatched specimens, in which the pituitary gland lacked the neurohypophysis. they were scarce ... | 1997 | 9310314 |
| localization of igf-i and igf-i receptor mrna in sparus aurata larvae. | studies of the ontogeny of igf-i mrna during embryonic and larval development of the gilthead sea bream sparus aurata showed its expression during these early developmental stages. the present study applies in situ hybridization to localize igf-i and igf receptor mrnas in 16-day larvae of s. aurata. paraffin sections were hybridized with homologous rna probes labeled by [35s]utp. igf-i mrna expression was found mainly in chondrocytes, in both the gill arches and cranial cartilage, in skeletal mu ... | 1997 | 9268610 |