| catfish hybrid ictalurus punctatus × i. furcatus exhibits higher resistance to columnaris disease than the parental species. | we present experimental data on susceptibility to columnaris disease, caused by the bacterium flavobacterium columnare, in hybrid catfish (female channel catfish ictalurus punctatus × male blue catfish i. furcatus) (c×b). under our experimental conditions, c×b hybrids were significantly more resistant to columnaris disease caused by the highly virulent strain of f. columnare bgfs-27 (genomovar ii) than channel catfish and blue catfish. channel and blue catfish cumulative mortalities after immers ... | 2012 | 22885516 |
| transcriptomic signatures of attachment, nf-κb suppression and ifn stimulation in the catfish gill following columnaris bacterial infection. | outbreaks of columnaris disease (flavobacterium columnare) are common in wild and cultured freshwater fish worldwide. disease occurrences, particularly those caused by virulent genomovar ii isolates, in aquaculture species such as channel catfish can be devastating. in contrast to other important aquaculture pathogens, little is known about host immune responses to columnaris. adhesion of f. columnare to gill tissue has been correlated in some previous studies to virulence and host susceptibilit ... | 2012 | 22669032 |
| susceptibility of fish and turtles to three ranaviruses isolated from different ectothermic vertebrate classes. | ranaviruses have been associated with mortality of lower vertebrates around the world. frog virus 3 (fv3)-like ranaviruses have been isolated from different ectothermic vertebrate classes; however, few studies have demonstrated whether this pathogen can be transmitted among classes. using fv3-like ranaviruses isolated from the american bullfrog lithobates catesbeianus, eastern box turtle terrapene carolina carolina, and pallid sturgeon scaphirhynchus albus, we tested for the occurrence of interc ... | 2014 | 24895866 |
| a leukocyte immune-type receptor subset is a marker of antiviral cytotoxic cells in channel catfish, ictalurus punctatus. | channel catfish, ictalurus punctatus, leukocyte immune type receptors (litrs) represent a multigene family that encodes ig superfamily proteins that mediate activating or inhibitory signaling. in this study, we demonstrate the use of mab cc41 to monitor viral cytotoxic responses in catfish and determine that cc41 binds to a subset of litrs on the surface of catfish clonal ctls. homozygous gynogenetic catfish were immunized with channel catfish virus (ccv)-infected mhc-matched clonal t cells (g14 ... | 2016 | 26856701 |
| development and evaluation of a loop-mediated isothermal amplification assay for the detection of channel catfish virus. | | 2015 | 25594335 |
| detection of channel catfish virus in cage-cultured pangasius hypophthalmus (sauvage, 1878) in malaysia. | | 2014 | 24117659 |
| establishment, characterization and viral susceptibility of two cell lines derived from leopard wrasse macropharyngodon geoffroy. | two new fish cell lines were established from skin (lwsk) and fin (lwfn) of leopard wrasse macropharyngodon geoffroy. these cells grew optimally at 25° c in leibovitz-15 medium supplemented with 10% foetal bovine serum. proliferation of m. geoffroy cells remained serum dependent up to cell passage 16, and cell-plating efficiency ranged from 12 to 16%. karyotypic analysis of these new cell lines at cell passage 8 indicated that both cell lines remained diploid with a peak chromosomal count of 144 ... | 2013 | 23991874 |
| pathogen recognition receptors in channel catfish: ii. identification, phylogeny and expression of retinoic acid-inducible gene i (rig-i)-like receptors (rlrs). | vertebrates including teleost fish have evolved an array of pathogen recognition receptors (prrs) for detecting and responding to various pathogen-associated molecular patterns (pamps), including toll-like receptors (tlrs), nucleotide-binding domain, leucine-rich repeat containing receptors (nlrs), and the retinoic acid inducible gene i (rig-i) like receptors (rlrs). as a part of the series of studies targeted to characterize catfish prrs, we described 22 nlr receptors in the sister contribution ... | 2012 | 22387588 |
| microbial quality and safety of x-ray irradiated fresh catfish (ictalurus punctatus) fillets stored under co2 atmosphere. | the effect of low-dose x-ray irradiation (0, 2, and 3 kgy) on pathogens, spoilage bacteria (psychrotrophic, anaerobic, and lactic acid bacteria), and physicochemical parameters of iced catfish fillets stored under carbon dioxide (co(2) ) atmosphere were studied. irradiation of 2 or 3 kgy eliminated all listeria monocytogenes (4.8 log cfu/g) and salmonella typhimurium (4.7 log cfu/g) while co(2) atmosphere alone showed less than 1 log reduction on salmonella typhimurium. there was no recovery of ... | 2012 | 22901245 |
| ontogenetic characterization of the intestinal microbiota of channel catfish through 16s rrna gene sequencing reveals insights on temporal shifts and the influence of environmental microbes. | aquaculture recently overtook capture fisheries as the largest producer of food fish, but to continue increasing fish production the industry is in search of better methods of improving fish health and growth. pre- and probiotic supplementation has gained attention as a means of solving these issues, however, for such approaches to be successful, we must first gain a more holistic understanding of the factors influencing the microbial communities present in the intestines of fish. in this study, ... | 2016 | 27846300 |
| characterization of the gut microbiota of three commercially valuable warmwater fish species. | due to the strong influence of the gut microbiota on fish health, dominant bacterial species in the gut are strong candidates for probiotics. this study aimed to characterize the gut microbiota of channel catfish ictalurus punctatus, largemouth bass micropterus salmoides and bluegill lepomis macrochirus to provide a baseline for future probiotic studies. | 2014 | 24529218 |
| prosthenystera oonastica n. sp. (digenea: callodistomidae) from ictalurid catfishes in southeastern united states and molecular evidence differentiating species in the genus across americas. | prosthenhystera oonastica n. sp. is described as a cryptic species from the gall bladder of three ictalurid catfishes, ictalurus punctatus (rafinesque), ictalurus furcatus (valenciennes), and pylodictis olivaris (rafinesque), in rivers in the southeastern united states. the species was originally named by wilmer a. rogers in 1979 but never formally described. material used for the description consists of two specimens of roger's original material and ten new specimens. we found no significant mo ... | 2015 | 25557746 |
| [connection of hepcidin genes from two fish species and their expression in pichia pastoris]. | hepcidin are small cationic peptides with antibacterial activity expressed mainly in the liver of living organisms, and they play important roles in the host's immune response against microbial invasion and regulation of iron metabolism. thus, they are considered to be good substitutes for traditional antibiotics. it is a good choice that the antimicrobial peptides are prepared by recombinant dna expression. in the present study, two hepcidin mature peptide cdnas from channel catfish (ictalurus ... | 2015 | 26571689 |
| suppression and restoration of primordial germ cell marker gene expression in channel catfish, ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: potential for reversible transgenic sterilization. | complementary dna overexpression and short hairpin rna interference approaches were evaluated for decreasing expression of primordial germ cell (pgc) marker genes and thereby sterilizing channel catfish, ictalurus punctatus, by delivering knockdown constructs driven by a constitutive promoter from yeast and a copper transport protein gene into fish embryos by electroporation. two pgc marker genes, nanos and dead end, were the target knockdown genes, and their expressions, along with that of an o ... | 2015 | 26341409 |
| a dietary dairy/yeast prebiotic and flaxseed oil enhance growth, hematological and immunological parameters in channel catfish at a suboptimal temperature (15°c). | channel catfish raised in the southern united states require two growing seasons to reach market size. growing seasons are separated by a cool period of about 3 months when feed intake and growth are greatly reduced. a cool-weather feeding strategy to improve feed intake, growth or health of catfish might improve survival and reduce the time needed to achieve market size. we conducted a feeding trial with channel catfish at a suboptimal temperature (15°c) to determine the effects of supplementin ... | 2015 | 25743337 |
| effects of transgenic sterilization constructs and their repressor compounds on hatch, developmental rate and early survival of electroporated channel catfish embryos and fry. | channel catfish (ictalurus punctatus) embryos were electroporated with sterilization constructs targeting primordial germ cell proteins or with buffer. some embryos then were treated with repressor compounds, cadmium chloride, copper sulfate, sodium chloride or doxycycline, to prevent expression of the transgene constructs. promoters included channel catfish nanos and vasa, salmon transferrin (tf), modified yeast saccharomyces cerevisiae copper transport protein (mctr) and zebrafish racemase (rm ... | 2015 | 25367204 |
| adaptation and cultivation of permanent fish cell line cco in serum-free medium and influence of protein hydrolysates on growth performance. | in this work we describe the adaptation of channel catfish ovary (cco) cell line to commercially available ultra culture serum-free medium by gradual reduction of serum concentration from 10 to 0 %. with this approach we obtained cco cells fully adapted to serum-free conditions in 32 days. growth, nutritional and morphological characteristics of these cells remained unchanged when compared to the control group kept in the presence of serum. additionally, three commercially available protein hydr ... | 2016 | 24993608 |
| effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (ictalurus punctatus). | a study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3 % for 7 weeks. each diet was provided to 10 channel catfish specimens (5.82 ± 0.13 g initial weight) replicated 3 times in individual 250 l fiberglass tanks. some hematologic parameters, leukocyte phagocytic activity, and intestinal morphology w ... | 2012 | 22437370 |
| genome sequence of the fish pathogen yersinia ruckeri sc09 provides insights into niche adaptation and pathogenic mechanism. | yersinia ruckeri is the etiologic agent of enteric red mouth disease (erm), a severe fish disease prevailing in worldwide aquaculture industries. here we report for the first time the complete genome of y. ruckeri (yersinia ruckeri) sc09, a highly virulent strain isolated from ictalurus punctatus with severe septicemia. sc09 possesses a single chromosome of 3,923,491 base pairs, which contains 3651 predicted protein coding sequences (cds), 19 rrna genes, and 79 trna genes. among the cds, we have ... | 2016 | 27089334 |
| the outer membrane proteins of stenotrophomonas maltophilia are potential vaccine candidates for channel catfish (ictalurus punctatus). | channel catfish (ictalurus punctatus) is an important agricultural fish that has been plagued by stenotrophomonas maltophilia (s. maltophilia) infections in recent years, some of them severe. the outer membrane proteins (omps) of s. maltophilia are one of the most immunogenic and highly conserved candidates for vaccine development in aquaculture. the present study investigated omps of s. maltophilia as vaccine on immune response and disease resistance against s. maltophilia of channel catfish an ... | 2016 | 27574827 |
| interleukin-8 holds promise to serve as a molecular adjuvant in dna vaccination model against streptococcus iniae infection in fish. | dna vaccines had been widely used in animal models against various viral infections, while it was not so convincing for many infectious diseases especially bacterial disease in aquaculture. interleukin-8(il-8) as one of the cxc chemokines, its immunological role and adjuvant potential which had been proved in mammals were rarely reported in fish species. in this study, recombination plasmid pcdna3.1/il-8(pcil-8) was conducted and the capacity of il-8 as molecular adjuvant was explored from sever ... | 2016 | 27911873 |
| cpsj gene of streptococcus iniae is involved in capsular polysaccharide synthesis and virulence. | the capsular polysaccharides are an important virulence factor of streptococcus iniae, protecting the bacterium from destruction and clearance by the immune system. the cpsj gene encodes a putative udp-glucose epimerase involved in the capsule synthesis system. to determine the role of the cpsj protein in the production of the capsule, a δcpsj mutant was generated and analyzed by comparing its growth performances and virulence with those of the wild type (wt) strain. the δcpsj mutant had longer ... | 2016 | 27535839 |
| molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (ictalurus punctatus) against streptococcus iniae. | interleukin-8 (il-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. however, there are fewer reports about the characterization and adjuvant effects of il-8 in fish. in this study, cloning and sequence analysis of il-8 coding region of channel catfish (ictalurus punctatus) were conducted, mature il-8(rtil-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine enco ... | 2016 | 27373470 |
| adjuvant immune enhancement of subunit vaccine encoding pscpi of streptococcus iniae in channel catfish (ictalurus punctatus). | channel catfish (ictalurus punctatus) is an important agricultural fish that has been plagued by streptococcus iniae (s. iniae) infections in recent years, some of them severe. c5a peptidase is an important virulent factor of s. iniae. in this study, the subunit vaccine containing the truncated part of c5a peptidase (pscpi) was mixed with aluminum hydroxide gel (ah), propolis adjuvant (pa), and freund's incomplete adjuvant (fia). the immunogenicity of the pscpi was detected by western-blot in vi ... | 2015 | 26602918 |
| channel catfish (ictalurus punctatus) protein disulphide isomerase, pdia6: molecular characterization and expression regulated by bacteria and virus inoculation. | protein disulfide isomerases (pdis) are thought to aid protein folding and assembly by catalyzing formation and shuffling of cysteine disulfide bonds in the endoplasmic reticulum (er). currently, increasing evidence suggests pdis play an important role in host cell invasion and they are relevant targets for the host immune response. however the roles of specific pdis in teleosts are little known. here, we characterized the protein disulfide isomerase family a, member 6 (pdia6) from channel catfi ... | 2012 | 22561356 |
| persistence of viral rna in fish infected with vhsv-ivb at 15°c and then moved to warmer temperatures after the onset of disease. | smallmouth bass, micropterus dolomieu lacepède, bluegill, lepomis macrochirus rafinesque (coppernose strain), koi carp, cyprinus carpio l., and channel catfish ictalurus punctatus (rafinesque), were infected by intraperitoneal injection with viral haemorrhagic septicaemia virus genotype ivb (vhsv-ivb) at 15 °c. when clinical signs of disease developed, one-third of the fish was moved to 20°c and one-third to 25°c. mortality in challenged fish at all three temperatures ranged from 25 to 45% in sm ... | 2012 | 22582793 |
| heterogeneity in levels of serum neutralizing antibodies against viral hemorrhagic septicemia virus genotype ivb among fish species in lake st. clair, michigan, usa. | the presence of neutralizing antibodies against viral hemorrhagic septicemia virus (vhsv-ivb) was investigated in sera of 13 fish species collected from lake st. clair, michigan, usa, a vhsv-endemic water body. we tested 297 sera collected may 2004-june of 2010, using a complement-dependent 50% plaque neutralization test (50% pnt). neutralizing antibodies were detected in 23% (67/297) of the samples. the highest overall antibody prevalence (85%, 34/40) and mean positive antibody titer (12,113 ± ... | 2012 | 22493115 |
| immune responses of channel catfish following the stimulation of three recombinant flagellins of yersinia ruckeri in vitro and in vivo. | innate immunity is initiated depending on the recognition of certain protein receptors termed pattern recognition receptors (prrs) of pathogen-associated molecular patterns (pamps) to protect the host from various invading pathogens. as one of the most powerful pamps, flagellin is the major structural component of the flagellum that provides the main force for bacterial motility in flagellated microorganisms. the genome of the y. ruckeri strain sc09 contains three flagellin genes, which encode t ... | 2017 | 28235583 |
| complete genome sequence of the fish pathogen yersinia ruckeri strain sc09, isolated from diseased ictalurus punctatus in china. | yersinia ruckeri sc09 is a gram-negative bacterium isolated from a moribund ictalurus punctatus collected in jianyang, china. here, we report the complete genome sequence of this microorganism to facilitate the investigation of its pathogenicity and to reevaluate its taxonomic position. | 2015 | 25573927 |
| a review of fish lectins. | lectins have been reported from various tissues of a diversity of fish species including japanese eel, conger eel, electric eel, bighead carp, gibel carp, grass carp, arabian gulf catfish, channel catfish, blue catfish, catfish, pike perch, perch, powan, zebrafish, toxic moray, cobia fish, steelhead trout, japanese trout, atlantic salmon, chinook salmon, olive rainbow smelt, rainbow smelt, white-spotted charr, tilapia, blue gourami, ayu, potca fish, spanish mackerel, gilt head bream, tench, roac ... | 2015 | 25929869 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| phylogenetic study of ameiurus melas based on complete mitochondrial dna sequence. | in our research, 16 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial dna (mtdna) of ameiurus melas. the total length of the mitochondrial genome is 16 512 bp and deposited in the genbank with accession no. kt804702. the gene arrangement and transcriptional direction were similar to other bony fishes which contained 37 genes (13 protein-coding genes, 2 ribosomal rna, and 22 transfer rnas) and a major non-coding control region. the g contents were ... | 2016 | 26679296 |
| vascular corrosion replicas of chemo-baroreceptors in fish: the carotid labyrinth in ictaluridae and clariidae. | scanning electron microscopy of vascular corrosion replicas and light microscopy revealed a pair of highly vascularized tissues, the carotid labyrinths, in the dorsal head region of the channel catfish, ictalurus punctatus, the black bullhead, i. melas, and the walking catfish, clarias batrachus. the labyrinth consists of a myriad of arterioles that arise from the common carotid artery immediately distal to the origin of the common carotid from the efferent branchial (epibranchial) artery of the ... | 1981 | 7273113 |
| comparative evaluation of the cytotoxicity sensitivity of six fish cell lines to four heavy metals in vitro. | to establish the potential use of cell cultures as a simple and sensitive biological tool to detect environmental pollutants, six cell lines established from several fish species including gcf (grass carp fins), cik (ctenopharyngodon idellus kidney), epc (epithelioma papulosum cyprini), cco (channel catfish ovary), bb (brown bullhead caudal trunk) and fhm (fathead minnow muscle) were tested and compared for their cytotoxic sensitivity to four heavy metals: cadmium (cd), chromium (cr), zinc (zn), ... | 2008 | 17931828 |
| conservation of a glutathione s-transferase in marine and freshwater fish. | we have previously reported the isolation and cloning of glutathione s-transferase (gst) cdnas from two marine fish, english sole (pleuronectes vetulus) and starry flounder (platichthys stellatus), that exhibited > 95% identity to plaice (pleuronectes platessa) gst-a aquatic toxicol., 44, 171-182]. in the present study, we have used reverse transcription-polymerase chain reaction (rt-pcr) analysis to isolate a 471 nucleotide gst-like cdna from largemouth bass (micropterus salmoides) liver. seque ... | 2003 | 11460686 |
| comparative metabolism and excretion of benzo(a)pyrene in 2 species of ictalurid catfish. | differential susceptibility of polycyclic aromatic hydrocarbon (pah)-mediated liver cancer exists in two related species of ictalurid catfish. two hypotheses are addressed in this study to explain this difference. specifically, the relatively insensitive channel catfish 1) do not produce mutagenic pah metabolites, and/or 2) they more quickly eliminate pahs due to greater phase ii enzyme activities than the more sensitive brown bullhead. livers and bile were collected from each species 6, 24, 72, ... | 2000 | 11053542 |
| the behavioral detection of binary mixtures of amino acids and their individual components by catfish. | the question of whether a binary mixture of amino acids is detected by fish as a unique odor or whether the qualities of the individual components are retained within the mixture was investigated in channel (ictalurus punctatus) and brown bullhead (ameiurus nebulosus) catfish, species that are highly similar in their olfactory receptor and behavioral responses to amino acid odorants. catfish respond with greater appetitive food-searching (swimming) behavior to amino-acid-conditioned olfactory st ... | 2000 | 11023851 |
| comparative in vitro and in vivo benzo[a]pyrene-dna adduct formation and its relationship to cyp1a activity in two species of ictalurid catfish. | we have measured the formation and persistence of benzo[a]pyrene (bap)-dna adducts in the liver of two closely related species of fish, the brown bullhead (ameriurus nebulosus) and the channel catfish (ictalurus punctatus) using the 32p-postlabeling method. liver microsomal ethoxyresorufin-o-deethylase (erod) activity, arylhydrocarbon hydroxylase (ahh) activity, and in vitro microsome-mediated dna binding were all significantly higher in the channel catfish. in an in vivo time-course experiment, ... | 1998 | 9512731 |
| glutathione-dependent defense in channel catfish (ictalurus punctatus) and brown bullhead (ameriurus nebulosus). | glutathione-dependent defense against xenobiotic toxicity is a multifaceted phenomenon that has been well characterized in mammals. this study undertakes a comparison of two benthic fish species, the channel catfish and brown bullhead, in terms of characteristics of the glutathione system. the channel catfish, a species that has seldom been observed to express pollutant-mediated neoplasia in field studies, was observed to have significantly higher constitutive levels of hepatic total glutathione ... | 1994 | 7523070 |
| sphaerospora infection of american catfishes (ictalurus punctatus and i. nebulosus) in europe. | the occurrence in europe of a sphaerospora species described in north america is reported. based upon its morphological characteristics, the parasite could be identified with the species s. hankai described from brown bullhead in canada. this parasite was found to infect channel catfish (ictalurus punctatus) cultured in farm ponds in italy and brown bullhead (ictalurus nebulosus) living in the supply channels of fish ponds in hungary. the spores and sporogonic developmental stages were situated ... | 1992 | 1298164 |
| organization of inner ear endorgan projections in the goldfish, carassius auratus. | cytoarchitectural analysis of the octavolateralis area of the goldfish, carassius auratus, reveals that as in other teleosts, five first-order octaval nuclei are present: the anterior octaval, magnocellular, descending, tangential, and posterior octaval nuclei. the descending nucleus appears to be anatomically specialized relative to that of the halecomorph amia calva and many teleosts in that a large dorsomedial subpopulation of the nucleus lies medial to nucleus medialis, a first-order lateral ... | 1994 | 8038983 |
| acanthocephala from lake fishes in wisconsin: ecology and host relationships of pomphorhynchus bulbocolli (pomphorhynchidae). | pomphorhynchus bulbocolli linkins in van cleave, 1919, was considerably more common in fishes of the river-connected tichigan lake than of the landlocked silver lake, southeastern wisconsin. it is reported from 17 species of principal, accessory, and occasional definitive hosts (new record in moxostoma carinatum) and from 13 species of paratenic hosts (new records in amia calva, ictalurus punctatus, lepomis cyanellus, and pomoxis nigromaculatus). infection patterns were influenced by fish specie ... | 1987 | 3585622 |
| characterization of cdna encoding immunoglobulin light chain of the mandarin fish (siniperca chuatsi). | immunoglobulin light chain cdna sequences of a perciform fish, the mandarin fish siniperca chuatsi were amplified from head kidney mrna by reverse transcription (rt)-pcr and race methods using degenerated primer and gene specific ones. in cdna sequences of the vl region, nucleotide exchanges were present mainly within cdrs, although a lesser degree of variability was also found in frs. moreover, the length of cdr1 and cdr3 in the mandarin fish is shorter than in most other fish species. in the m ... | 2003 | 12969639 |
| molecular cloning and characterization analysis of immunoglobulin m heavy chain gene in european eel (anguilla anguilla). | in this study, the immunoglobulin m heavy chain gene of european eel (anguilla anguilla) was cloned and analyzed. the full-length cdna of the igm heavy chain gene (genbank accession no. ef062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. the igm heavy chain was composed of leader peptide (l), variable domain (vh), ch1, ch2, hinge, ch3, ch4, and c-terminus and two novel continuous putative n-glycosylation sites were found close to the second cysteine of ch3 in a. anguil ... | 2009 | 19013650 |
| sequence and expression of androgen receptor and estrogen receptor gene in the sex types of protogynous wrasse, halichoeres trimaculatus. | sex steroid hormones play important roles in sex change and behavior of wrasses. their actions are considered to be mediated through the nuclear hormone receptors. in this study, to elucidate the roles of estrogen receptor (er) and androgen receptor (ar) in the reproduction of the protogynous wrasse, halichoeres trimaculatus, ar and er genes were partially cloned using 5'- and 3'-race and their transcript levels in the gonads and the brain were measured by competitive rt-pcr. the amino acid sequ ... | 2002 | 12383444 |
| in vivo and in vitro cyp1b mrna expression in channel catfish. | our goal was to study the induction of cyp1b mrna expression in channel catfish (ictalurus punctatus). cyp1b belongs to the cytochrome p450 superfamily of genes, is involved in the oxidation of endogenous and exogenous compounds, and could potentially be a useful biomarker in fish for exposure to ahr ligands. the full-length catfish cyp1b cdna is 2417 nt to the polya tail and encodes a putative protein of 536 amino acids. it has 67% amino acid similarity to carp and zebrafish cyp1b and 68% simil ... | 2006 | 16697458 |
| handling and storage procedures have variable effects on fatty acid content in fishes with different lipid quantities. | it is commonly assumed that the most accurate data on fatty acid (fa) contents are obtained when samples are analyzed immediately after collection. for logistical reasons, however, this is not always feasible and samples are often kept on ice or frozen at various temperatures and for diverse time periods. we quantified temporal changes of selected fa (μg fame per mg tissue dry weight) from 6 fish species subjected to 2 handling and 3 storage methods and compared them to fa contents from muscle t ... | 2016 | 27479304 |
| tissue alkaline phosphatase activity in selected freshwater teleosts. | 1. kinetic studies of tissue alkaline phosphatase activity were carried out on eight species of teleost fishes taken from the mississippi river. 2. liver alkaline phosphatase activity expressed in mumoles of p-nitrophenol released per minute per gram of protein were as follows: micropterus salmoides, 20.31; stizostedion vitreum, 19.22; pomoxis nigromaculatus, 18.76; lepisosteus platostomus, 16.26; esox lucius, 16.14; hiodon tergisus, 13.83; ictalurus punctatus, 10.87; aplodinotus grunniens, 7.94 ... | 1978 | 318337 |
| experimental bolbophorus damnificus (digenea: bolbophoridae) infections in piscivorous birds. | in order to determine potential definitive hosts of the digenetic trematode, bolbophorus damnificus, two american white pelicans (pelecanus erythrorhynchos), two double-crested cormorants (phalacrocorax auritus), two great blue herons (ardea herodias), and two great egrets (ardea alba) were captured, treated with praziquantel, and fed channel catfish (ictalurus punctatus) infected with b. damnificus metacercariae. patent infections of b. damnificus, which developed in both american white pelican ... | 2009 | 19617478 |
| evolution of gustatory reflex systems in the brainstems of fishes. | the great number of species of teleosts permits highly specialized forms to evolve to occupy particular niches. this diversity allows for extreme variations in brain structure according to particular sensory or motor adaptations. in the case of the taste system, goldfish (carassius auratus l., 1758) and some carps have evolved a specialized intraoral food-sorting apparatus along with corresponding specializations of gustatory centers in the brainstem. a comparison of circuitry within the complex ... | 2009 | 20160963 |
| descending neural projections to the spinal cord in the channel catfish, ictalurus punctatus. | retrograde transport of horseradish peroxidase was used to determine the descending projections to the spinal cord in an otophysan fish, the channel catfish, ictalurus punctatus. the majority of cells projecting to the spinal cord are located in the reticular formation, which is organized into rhombomeric segments. vestibulospinal neurons are located in the descending, magnocellular, and tangential octaval nuclei, as well as in the medial octavolateralis nucleus of the lateral line system. cells ... | 1998 | 9776078 |
| afferent and efferent connections of nucleus praeeminentialis in the channel catfish: a reevaluation. | nucleus praeeminentialis (npr) is an isthmic nucleus that has been described in the brains of electrosensory teleost fishes and a single non-electrosensory species. the nucleus receives axon collaterals of ascending medullary sensory lemniscal neurons. axons of npr neurons project in turn back down onto those same populations of medullary projection neurons via a descending parallel fiber system (the molecular layer or cerebellar crest). thus npr forms a link in a sensory feedback loop that modu ... | 1998 | 9553693 |
| connections of octaval and lateral line nuclei of the medulla in the goldfish, including the cytoarchitecture of the secondary octaval population in goldfish and catfish. | cytoarchitectural analyses combined with injections of the tracer horseradish peroxidase in various structures in the brain of the goldfish, carassius auratus, have defined some of the major components of acoustic and lateral line mechanosensory circuits between the medulla and midbrain. the main acoustic receptor in carassius, the saccule, is known to provide a major input to the dorsomedial zone of the descending octaval nucleus. the dorsomedial zone in turn projects bilaterally to the seconda ... | 1996 | 8680846 |
| diencephalic gustatory connections in the channel catfish. | vertebrate gustatory systems include a tertiary ascending pathway from a secondary gustatory nucleus in the hindbrain to several forebrain nuclei. this connection is prominent in catfish, corresponding to their highly developed sense of taste. iontophoretic injections of horseradish peroxidase were used to identify the specific target nuclei of the tertiary gustatory pathway in channel catfish and to characterize those nuclei by their respective connections. efferents from the secondary gustator ... | 1993 | 7506715 |
| the primary octaval nuclei and inner ear afferent projections in the otophysan ictalurus punctatus. | physical coupling between the swimbladder and the inner ear is believed to contribute to the enhanced hearing abilities of otophysans relative to those of teleosts lacking comparable otic specializations. we hypothesized that the auditory circuitry of otophysans might also exhibit derived features [mccormick and braford, 1988]. as a first test of this hypothesis, we examined the normal anatomy and inner ear inputs of the primary octaval nuclei in the catfish ictalurus punctatus. from nissl-stain ... | 1993 | 8324624 |
| ascending general visceral pathways within the brainstems of two teleost fishes: ictalurus punctatus and carassius auratus. | the primary general visceral nucleus in goldfish (carassius auratus) and catfish (ictalurus punctatus) is located at the ventroposterior boundary of the vagal gustatory lobe and receives coelomic visceral, but not gustatory inputs. the neuronal tracer horseradish peroxidase (hrp) was employed to visualize sources of input to and ascending projections from the primary general visceral nucleus in these species. in addition, immunocytochemical techniques were utilized to define the cytological divi ... | 1992 | 1629401 |
| afferent connections of the optic tectum in channel catfish ictalurus punctatus. | horseradish peroxidase was injected unilaterally into the optic tectum of the channel catfish, ictalurus punctatus. the sources of tectal afferents were thereby revealed by retrogradely labeled neurons in various brain centers. retrogradely labeled cells were seen in both the ipsilateral and contralateral telencephalon. the superficial pretectal area was labeled on both sides of the brain. ipsilateral projections were also observed coming from the entopeduncular nucleus. both the anterior thalam ... | 1990 | 2078856 |
| visual system of the channel catfish (ictalurus punctatus): iii. fiber order in the optic nerve and optic tract. | in channel catfish the ganglion cell axons leave the retina via a ring of approximately 13 separate optic papillae. each papilla serves an area of retina extending from the central zone of the retina to the periphery. papillae located at a dorsal position in the ring serve exclusively dorsal retina. ventrally located papillae, however, have an exaggerated peripheral retinal representation, so that they serve mostly ventral retina but also some areas of peripheral retina dorsal to the nasal and t ... | 1988 | 3360990 |
| central projections of the glossopharyngeal and vagal nerves in the channel catfish, ictalurus punctatus: clues to differential processing of visceral inputs. | transganglionic transport of horseradish peroxidase was used to trace the pattern of medullary terminations of the glossopharyngeal and vagal nerve complex in the channel catfish, ictalurus punctatus. the glossopharyngeal root terminates centrally in the anterior end of the vagal lobe except for two fascicles that terminate in separate regions of the nucleus intermedius of the facial lobe. vagal nerve branches innervating regions of the oropharynx terminate in an overlapping, segmental fashion t ... | 1987 | 3680629 |
| topography of retinodiencephalic projections in adult channel catfish. | in order to determine the topography of retinal axons in diencephalic nuclei of ictalurus punctatus, horseradish peroxidase was applied to the optic papillae. the topography was found to be similar in all nuclei in that the terminal endings of ventral retinal axons innervated rostrodorsally whereas dorsal retinal axons innervated caudoventrally. in general, the retinal topography in the diencephalon corresponded with the topography of retinal axons in the optic tracts. these data suggest a relat ... | 1986 | 3768716 |
| the visual system of the channel catfish (ictalurus punctatus). i. retinal ganglion cell morphology. | horseradish peroxidase was applied to lesions in the optic nerve of catfish (ictalurus punctatus). the retinae were processed to reveal hrp-labelled ganglion cells. the histochemical techniques employed allowed fine details of the dendritic arbor to be resolved. flat-mounted retinae were examined and the following characteristics were noted in individual ganglion cells: soma area, shape, and depth; number and diameter of major dendrites; shape, area, and depth(s) within the inner plexiform layer ... | 1986 | 3711375 |
| three distinct morphological classes of receptors in fish olfactory organs. | three morphologically distinct classes of receptor-neurons are proposed: (1) type i ciliar cells, (2) microvillar cells and (3) type ii ciliar cells. retrograde transport of horseradish peroxidase by axons in the olfactory nerve to the olfactory organs of goldfish (carassius auratus) and channel catfish (ictalurus punctatus) provided evidence that these axon-bearing cells are present in the organs of both species. goldfish olfactory organs were also studied with scanning electron microscopy, dis ... | 1984 | 6199384 |
| the ipsilateral retinotectal projection in normal and albino channel catfish. | the retinal projection to the ipsilateral optic tectum was examined first in normal channel catfish. after unilateral optic nerve section and horseradish peroxidase (hrp) application, a sparse retinal projection was seen in the ipsilateral optic tectum. when hrp was applied to the ipsilateral rostral tectum, labeled ganglion cells were observed in the ventrotemporal quadrant of the ipsilateral eye. electrophysiological mapping of the ipsilateral projection confirmed that cells in the temporal re ... | 1983 | 6856199 |
| retinofugal pathways in juvenile and adult channel catfish, ictalurus (ameiurus) punctatus: an hrp and autoradiographic study. | the retinal projections of the juvenile and adult channel catfish, ictalurus (ameiurus) punctatus, were studied by using horseradish peroxidase (hrp) and autoradiography. the contralateral optic tract sends fibers to the suprachiasmatic nucleus (scn) and divides into lateral (lot) and medial optic tracts (mot). in the adult fish, the former is thicker than the latter, whereas in the juvenile form, the reverse is true. the mot curves laterally and divides into eight to 15 medial fascicles of the ... | 1982 | 7130469 |
| telencephalic efferents in channel catfish, ictalurus punctatus: projections to the olfactory bulb and optic tectum. | telencephalic projections to the olfactory bulb and optic tectum were examined with horseradish peroxidase (hrp) methods in catfish. olfactory bulb afferents arise from the mitral cell layer of the contralateral olfactory bulb and the ipsilateral telencephalon. the majority of labelled cells appear within the ventral and dorsal nuclei of area ventralis telencephali and two divisions of the central zone of area dorsalis telencephali. excepting one division of the dorsal central zone, all afferent ... | 1981 | 7326567 |
| molecular immune response of channel catfish immunized with live theronts of ichthyophthirius multifiliis. | the parasite ichthyophthirius multifiliis (ich) has been reported in various freshwater fishes worldwide and results in severe losses to both food and aquarium fish production. the fish surviving natural infections or immunized with live theronts develop strong specific and non-specific immune responses. little is known about how these immune genes are induced or how they interact and lead to specific immunity against ichthyophthirius multifiliis in channel catfish ictalurus punctatus. this stud ... | 2016 | 27044331 |
| residues of organochlorine insecticides, polychlorinated biphenyls, and heavy metals in biota from apalachicola river, florida, 1978. | seventy-seven composite samples composed of largemouth bass ( micropterus salmoides ), channel catfish ( ictaluras punctatus), threadfin shad ( dorosoma petenense ), asiatic clam (corbicula fluminea ), burrowing mayfly ( hexagenia sp.), water snake ( natrix spp.), and little green heron ( butorides virescens ) were collected from upper and lower reaches of the apalachicola river, florida, in 1978 for residue analysis of organochlorine insecticides, pcbs (polychlorinated biphenyls), and metals. c ... | 2013 | 6427177 |
| serotonergic synapses modulate generation of spikes from retinal ganglion cells of teleosts. | serotonin [5-hydroxytryptamine (5-ht)] is a common neurotransmitter/neuromodulator found widely in the nervous system. cellular morphology and retinal distribution of serotonergic amacrine cells in the channel catfish (ictalurus punctatus) retina are identified using monoclonal anti-5ht antibody. these cells receive ribbon synapses from off-center (hyperpolarizing) bipolar cells as well as conventional synapses with other non-serotonergic amacrine cells. output synapses from the serotonergic cel ... | 2009 | 19938208 |
| fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish. | there is a need to develop simple, rapid, and accurate methods for assessing health in fish populations. in this study we demonstrate that use of fluorescein, a nontoxic fluorescent dye, can rapidly and easily detect the presence of skin ulcers in all fish tested, including rainbow trout (oncorhynchus mykiss), channel catfish (ictalurus punctatus), goldfish (carassius auratus), and hybrid striped bass (morone saxatilis male x m. chrysops female). exposure of fish to as little as 0.10 mg fluoresc ... | 2002 | 12450204 |
| complete nucleotide sequence of the cytochrome b gene of channel catfish ictalurus punctatus and comparison of sequence homology among channel catfish and other fishes. | to clarify the phylogenic relationship of channel catfish with other fishes, the cytochrome b (cyt b) gene of the catfish was cloned and sequenced. channel catfish (ictalurus punctatus) belonging to the family ictaluridae in the order siluriformes showed a 78.4-87.4% similarity to all but one fish of the family cyprinidae and river loach crossostoma lacustre of the family balitoridae in the order cypriniformes in which genes had already been sequenced, and a 97.2% similarity to the goldfish (car ... | 2001 | 11258463 |
| bifurcation analysis of nonlinear retinal horizontal cell models. i. properties of isolated cells. | 1. bifurcation theory is used to study properties of nonlinear analytical and computational models of isolated retinal horizontal cells. the analytical model is based on the published data of shingai and christensen describing steady-state i-v characteristics of horizontal cells isolated from catfish (ictalurus punctatus) retina. the computational model is based on i-v characteristics of distinct macroscopic membrane currents observed in horizontal cells isolated from goldfish (carassius auratus ... | 1989 | 2769357 |
| acute toxicity of nitrofurazone to channel catfish, ictalurus punctatus, and goldfish, carassius auratus. | | 1987 | 3814851 |
| molecular cloning, sequencing and tissue expression of vasotocin and isotocin precursor genes from ostariophysian catfishes: phylogeny and evolutionary considerations in teleosts. | basic and neutral neurohypophyseal (nh) nonapeptides have evolved from vasotocin (vt) by a gene duplication at the base of the gnathostome lineage. in teleosts, vt and it are the basic and neutral peptides, respectively. in the present study, vt and it precursor genes of heteropneustes fossilis and clarias batrachus (siluriformes, ostariophysi) were cloned and sequenced. the channel catfish icatalurus punctatus nh precursor sequences were obtained from est database. the catfish nh sequences were ... | 2015 | 26029040 |
| comparison of cytotoxicity induced by 17α-ethynylestradiol and diethylstilbestrol in fish cco and mammalian cho-k1 cell lines. | the effects of synthetic estrogens 17α-ethynylestradiol (ee2) and diethylstilbestrol (des) were compared on cell proliferation and morphology in channel catfish ovary (cco) and chinese hamster ovary (cho-k1) cells. ee2 exposure (0.1 and 0.5 μg/ml) induced stimulatory effect on cco and cho-k1 cell proliferation, while higher concentrations (1-10 μg/ml) showed cytotoxic effects. increase in des concentrations mainly resulted in dose-depended increase in cytotoxicity in both cell lines. morphologic ... | 2011 | 21327609 |
| physiological responses to short-term fasting among herbivorous, omnivorous, and carnivorous fishes. | we explored the integrated role of dietary specialization and feeding periodicity on the response of the gastrointestinal tract of teleosts fishes to short-term (7-10 days) fasting and refeeding. fasted and fed herbivorous grass carp (ctenopharyngodon idella), omnivorous channel catfish (ictalurus punctatus), and carnivorous largemouth bass (micropterus salmoides) were compared for digestive organ masses, intestinal morphology, gastrointestinal ph, and the specific activities and total intestina ... | 2014 | 24604291 |
| ig heavy chain genes and their locus in grass carp ctenopharyngodon idella. | the cdna and genomic sequences of igd and igz were characterized in grass carp ctenopharyngodon idella in the present study, and with the identification of a bac clone covering zeta, mu, and delta genes, the igh locus containing these ig genes and other v, d, j genes was also illustrated in this fish. secretory and membrane-bound igz were identified, with two transmembrane exons spliced within the ch4 exon, as reported in igm of mammals and igz in other teleost fish. the first and second constan ... | 2010 | 20558301 |
| infection and immunity against ichthyophthirius multifiliis in zebrafish (danio rerio). | ichthyophthirius multifiliis, causing white spot disease, is a serious pathogen in aquaculture as well as for the ornamental fish industry. in carp, channel catfish and rainbow trout the immune responses against the parasite have been partly elucidated and these species are able to acquire a high level of immunity against the disease. zebrafish are however, known to be more resilient towards the disease than channel catfish but the pathology and the ability to obtain protection is unknown. in th ... | 2016 | 27567935 |
| determination of eugenol in fish and shrimp muscle tissue by stable isotope dilution assay and solid-phase extraction coupled gas chromatography-triple quadrupole mass spectrometry. | in this study, we developed a new method for the accurate quantification of eugenol in fish samples based on stable isotope dilution assay (sida) and solid-phase extraction (spe) coupled gas chromatography-triple quadrupole mass spectrometry (sida-spe-gc-ms/ms). due to the difference of matrix effect (me), it was difficult to determine accurately the level of eugenol residue in different fish and shrimp samples based on external standard calibration method. sida was applied to compensate matrix ... | 2016 | 27531030 |
| editing of the luteinizing hormone gene to sterilize channel catfish, ictalurus punctatus, using a modified zinc finger nuclease technology with electroporation. | channel catfish (ictalurus punctatus) is the most important freshwater aquaculture species in the usa. genetically enhanced fish that are sterile could both profit the catfish industry and reduce potential environmental and ecological risks. as the first step to generate sterile channel catfish, three sets of zinc finger nuclease (zfn) plasmids targeting the luteinizing hormone (lh) gene were designed and electroporated into one-cell embryos, different concentrations were introduced, and the cel ... | 2016 | 26846523 |
| temperature and species-specific effects on ß3-adrenergic receptor cardiac regulation in two freshwater teleosts: channel catfish (ictalurus punctatus) and common carp (cyprinus carpio). | β₃-adrenergic receptors (ar) are important in teleost cardiovascular regulation. to date, it is unknown whether temperature acclimation changes ß₃-ar functionality and consequently the involvement of this ar subtype in teleost cardiac regulation. common carp (cyprinus carpio) were acclimated at 12 °c or 23 °c (minimum 3 weeks) after which cardiovascular variables (cardiac output (q), stroke volume (sv) and heart rate (fh)) were measured upon injection of the ß₃-ar agonist, brl(37344), and antago ... | 2015 | 25882086 |
| effectiveness of a sediment time critical removal action-pcb reduction in fish tissue, surface water, and sediment via wet excavation. | documenting successful remediation of polychlorinated biphenyl (pcb)-contaminated sediments is limited; potentially due to inadequate monitoring methods, complexities associated with the environment, and selected remedial techniques. at some sites, absence of appropriate baseline and postremoval monitoring limits proper evaluation of remedial efficacy. accurate interpretation of interactions between media, space, time, species, lipid content, and remedial technique requires robust study design a ... | 2015 | 25070026 |
| polybrominated diphenyl ether metabolism in field collected fish from the gila river, arizona, usa--levels, possible sources, and patterns. | polybrominated diphenyl ethers (pbdes) were determined in fish collected from the gila river, arizona, a tributary of the colorado river in the lower part of the colorado river basin. fish samples were collected at sites on the gila river downstream from hayden, phoenix, and arlington, arizona in late summer 2003. the gila river is ephemeral upstream of the phoenix urban area due to dams and irrigation projects and has limited perennial flow downstream of phoenix due to wastewater and irrigation ... | 2013 | 22939514 |
| detection of temporal trends of α- and γ-chlordane in lake erie fish communities using dynamic linear modeling. | dynamic linear modeling (dlm) analysis was performed to identify the long-term temporal trends of two toxic components of the technical chlordane pesticide, α- and γ-chlordane, in skinless-boneless muscle tissues of a number of sport fish species in lake erie. our analysis considers the fish length as a covariate of the chlordane concentrations. the α-chlordane models for the coho salmon, channel catfish, rainbow trout, and common carp showed continuously decreasing trends during the entire 30+ ... | 2011 | 21536330 |
| characterization of a mannose-binding lectin from channel catfish (ictalurus punctatus). | mannose-binding lectin (mbl) is an important component of innate immunity capable of activating the lectin pathway of the complement system. a mbl gene was isolated from channel catfish (ictalurus punctatus). the deduced protein contains a canonical collagen-like domain, a carbohydrate recognition domain (crd), and a neck region similar to mammalian mannose-binding lectin. the catfish mannose-binding lectin crd contains the epn motif shown previously to mediate mannose specificity. the catfish m ... | 2012 | 21524427 |
| molecular characterization of complement factor i reveals constitutive expression in channel catfish. | the complement system in vertebrates plays a crucial role in immune defense via recognition and removal of pathogens. complement is tightly regulated by a group of both soluble and cell-associated proteins. complement factor i is a soluble serine protease that regulates multiple pathways in complement activation. in this work, a complement factor i transcript was isolated and sequenced from channel catfish (ictalurus punctatus) liver after screening expressed sequence tags. the full-length cdna ... | 2009 | 19540919 |
| chemical contaminants, health indicators, and reproductive biomarker responses in fish from the colorado river and its tributaries. | common carp (cyprinus carpio), black bass (micropterus spp.), and channel catfish (ictalurus punctatus) were collected from 14 sites in the colorado river basin (crb) to document spatial trends in accumulative contaminants, health indicators, and reproductive biomarkers. organochlorine residues, 2,3,7,8-tetrachlorodibenzo-p-dioxin-like activity (tcdd-eq), and elemental contaminants were measured in composite samples of whole fish, grouped by species and gender, from each site. selenium (se) and ... | 2007 | 17418376 |
| mercury concentrations in fish from lake meredith, texas: implications for the issuance of fish consumption advisories. | we examined how length of fish is related to mercury concentrations in muscle tissue of seven species of fish from lake meredith, texas and determined how sex and growth rate are related to mercury concentration in walleye (sander vitreus). flathead catfish (pylodictis olivaris), walleye and white bass (morone chrysops) had the highest concentrations of mercury and channel catfish (ictalurus punctatus), common carp (cyprinus carpio), river carpsucker (carpiodes carpio) and gizzard shad (dorosoma ... | 2006 | 17054010 |
| use of the p,p'-ddd: p,p'-dde concentration ratio to trace contaminant migration from a hazardous waste site. | for approximately 50 years, beginning in the 1920s, hazardous wastes were disposed in an 11-hectare area of the marine corps base (mcb) quantico, virginia, usa known as the old landfill. polychlorinated biphenyls (pcbs) and ddt compounds were the primary contaminants of concern. these contaminants migrated into the sediments of a 78-hectare area of the potomac river, the quantico embayment. fish tissue contamination resulted in the mcb posting signs along the embayment shoreline warning fisherme ... | 2006 | 16758288 |
| biochemical effects of lead, zinc, and cadmium from mining on fish in the tri-states district of northeastern oklahoma, usa. | we assessed the exposure of fish from the spring and neosho rivers in northeast oklahoma, usa, to lead, zinc, and cadmium from historical mining in the tri-states mining district (tsmd). fish (n = 74) representing six species were collected in october 2001 from six sites on the spring and neosho rivers influenced to differing degrees by mining. additional samples were obtained from the big river, a heavily contaminated stream in eastern missouri, usa, and from reference sites. blood from each fi ... | 2005 | 16117126 |
| concentrations of cadmium, lead, and zinc in fish from mining-influenced waters of northeastern oklahoma: sampling of blood, carcass, and liver for aquatic biomonitoring. | the tri-states mining district (tsmd) of missouri (mo), kansas (ks), and oklahoma (ok), usa, was mined for lead (pb) and zinc (zn) for more than a century. mining ceased more than 30 years ago, but wastes remain widely distributed in the region, and there is evidence of surface- and groundwater contamination in the spring river-neosho river (sr-nr) system of northeastern ok. in october 2001, we collected a total of 74 fish from six locations in the sr-nr system that included common carp (cyprinu ... | 2005 | 15981034 |
| molecular cloning of proopiomelanocortin cdna and multi-tissue mrna expression in channel catfish. | channel catfish (ictalurus punctatus) proopiomelanocortin (pomc) cdna was cloned to investigate its structure, evolution, and expression in different tissues. pomc is an important gene in the hypothalamus-pituitary-adrenal axis, the main mediator of the stress response. pomc gene was isolated from a pituitary cdna library and nucleotide sequence was determined. pomc cdna is composed of 1164 nucleotides with a 639 nucleotide open reading frame encoding a protein of 212 amino acids. catfish pomc p ... | 2004 | 15201069 |
| channel catfish follicle-stimulating hormone and luteinizing hormone: complementary dna cloning and expression during ovulation. | gonadotropins are important regulators of reproduction. to develop molecular resources for production of recombinant gonadotropins, we have cloned and sequenced complementary dna encoding the channel catfish (ictalurus punctatus) follicle-stimulating hormone (fsh) and luteinizing hormone (lh), which encode 132 and 140 amino acid proteins, respectively. the deduced amino acid sequences of the catfish fsh and lh are highly conserved with those cloned from other teleosts. both the fsh and the lh we ... | 2001 | 14961331 |
| molecular identification and expression analysis of tumor necrosis factor in channel catfish (ictalurus punctatus). | a tumor necrosis factor (tnf) alpha-like gene, encoding a propeptide of 230 amino acids and a mature (soluble) peptide of 162 amino acids, was identified in channel catfish (ictalurus punctatus). while the catfish protein shared features in common with both mammalian tnfalpha and tnfbeta homologs, overall sequence identity/similarity was slightly higher vs. tnfalpha genes when mature tnf sequences were compared. phylogenetic analysis placed catfish and other fish tnf sequences within their own c ... | 2003 | 12880635 |
| bioavailability and toxicity of dietborne copper and zinc to fish. | to date, most researchers have used dietborne metal concentrations rather than daily doses to define metal exposure and this has resulted in contradictory data within and between fish species. it has also resulted in the impression that high concentrations of dietborne cu and zn (e.g. > 900 mg kg(-1) dry diet) are relatively non-toxic to fish. we re-analyzed existing data using rations and dietborne metal concentrations and used daily dose, species and life stage to define the toxicity of dietbo ... | 2002 | 12161165 |
| inhibition of erythrocyte delta-aminolevulinic acid dehydratase (alad) activity in fish from waters affected by lead smelters. | we assessed the effects on fish of lead (pb) released to streams by smelters located in trail, bc (canada), e. helena, mt, herculaneum, mo, and glover, mo. fish were collected by electrofishing from sites located downstream of smelters and from reference sites. blood from each fish was analyzed for delta-aminolevulinic acid dehydratase (alad) activity and hemoglobin (hb), and samples of blood, liver, or carcass were analyzed for pb, zinc (zn), or both. fish collected downstream of all four smelt ... | 2002 | 12139078 |
| ethoxyresorufin-o-deethylase (erod) activity in fish as a biomarker of chemical exposure. | this review compiles and evaluates existing scientific information on the use, limitations, and procedural considerations for erod activity (a catalytic measurement of cytochrome p4501a induction) as a biomarker in fish. a multitude of chemicals induce erod activity in a variety of fish species, the most potent inducers being structural analogs of 2,3,7,8-tetracholordibenzo-p-dioxin. although certain chemicals may inhibit erod induction/activity, this interference is generally not a drawback to ... | 2000 | 10955715 |
| transcriptional activities in the pituitaries of channel catfish before and after induced ovulation by injection of carp pituitary extract as revealed by expressed sequence tag analysis. | expressed sequence tag (est) analysis was adopted to address physiological changes after injection of carp pituitary extract for induction of ovulation. ests were analyzed from cdna libraries constructed from mrna isolated from channel catfish (ictalurus punctatus) pituitaries before and after induction of ovulation by injection of carp pituitary extract. one hundred randomly picked clones were analyzed. of the sequences generated, a large percentage (59%) of ests were identified as known genes ... | 1998 | 9801455 |
| gonadotropin alpha-subunit glycoprotein from channel catfish (ictalurus punctatus) and its expression during hormone-induced ovulation. | complementary dna (cdna) encoding the channel catfish (ictalurus punctatus) gonadotropin (gth) alpha-subunit glycoprotein was cloned by polymerase chain reaction (pcr) from a plasmid library made from pituitary rna. complete cdna cloning was achieved by carrying out two pcr reactions: one with an upstream sense primer plus the universal sequencing primer, located downstream of the poly(a) sequence of the cdna in the plasmid vector, to amplify the downstream portion of the cdna; the other with a ... | 1997 | 9284560 |
| characterization of hepatic metallothionein expression in channel catfish (ictalurus punctatus) by reverse transcriptase polymerase chain reaction. | measurements of metallothioneins (mts) can be labour intensive and require expensive probes and or analytical instrumentation. in contrast, the reverse transcriptase polymerase chain reaction (rt-pcr0 has provided a relatively sensitive and simple mechanism to measure expression of mt in sentinel species in aquatic environments. the objectives of this study were to characterize expression of hepatic mt in a commonly occurring fish species of the southeastern united states, the channel catfish (i ... | 1997 | 23899176 |
| nutrition of aquaculture species. | dietary requirements for amino acids and fatty acids have been reported for channel catfish (ictalurus punctatus), salmonids (oncorhynchus spp.), common carp (cyprinus carpio), tilapias (oreochromis spp.), and eel (anguilla japonicus). most of the vitamin and mineral requirements are available for channel catfish and salmonids, and some are available for common carp, tilapia, eel, and other finfish and crustaceans. from this available information, cost-effective feeds can be formulated for the m ... | 1991 | 1778835 |