| effects of inhaled heparin on immunologic and nonimmunologic bronchoconstrictor responses in sheep. | the glycosaminoglycan heparin has been shown to block inositol triphosphate (ip3) receptors in various tissues. because ip3 is one of the pathways involved in stimulus-secretion coupling in mast cells, it is possible that heparin may inhibit airway anaphylaxis. thus, the purpose of the present investigation was to study the effect of inhaled heparin on immunologically and nonimmunologically induced bronchoconstriction in allergic sheep. specific lung resistance (srl) was measured in 15 sheep all ... | 1992 | 1546836 |
| the mitochondrial genomes of two nematodes, caenorhabditis elegans and ascaris suum. | the nucleotide sequences of the mitochondrial dna (mtdna) molecules of two nematodes, caenorhabditis elegans [13,794 nucleotide pairs (ntp)], and ascaris suum (14,284 ntp) are presented and compared. each molecule contains the genes for two ribosomal rnas (s-rrna and l-rrna), 22 transfer rnas (trnas) and 12 proteins, all of which are transcribed in the same direction. the protein genes are the same as 12 of the 13 protein genes found in other metazoan mtdnas: cyt b, cytochrome b; coi-iii, cytoch ... | 1992 | 1551572 |
| motor behavior and motor nervous system function in the nematode ascaris suum. | ascaris suum has a nervous system that is very simple both numerically and morphologically. it comprises only 298 neurons almost all of which are extremely simple in shape. extensive anatomical descriptions of the morphology of neurons and of their synaptic connections, together with the study, by using intracellular recording techniques, of their physiological properties, have led to a prediction of how the motor nervous system controls behavior. subsequent discovery of endogenous neuropeptides ... | 1992 | 1556637 |
| comparison between the mhc-restricted antibody repertoire to ascaris antigens in adjuvant-assisted immunization or infection. | genetic restrictions to the immune repertoire will be an important consideration in the development of anti-nematode vaccines. it has already been established that the major histocompatibility complex (mhc) limits responsiveness to nematode antigens in infection, but little is known of whether this also applies under other routes of sensitization, such as with adjuvants. the specificity of the antibody response was, therefore, compared in infection and adjuvant-assisted immunization using secret ... | 1992 | 1557231 |
| helminthiasis and cultural change in the peruvian rainforest. | the objective of this study was to identify the impact of cultural and ecological change on intestinal helminth infections in traditional indigenous communities in the peruvian rainforest and to identify the ways of transmission of helminth eggs. a remote indigenous settlement was compared with a more developed mestizo town. stool specimens as well as soil, dust, air and water were examined for ascaris and trichuris eggs. a significantly lower infection intensity of ascaris and trichuris could b ... | 1992 | 1560477 |
| soil contamination with ascaris lumbricoides eggs as an indicator of environmental hygiene in urban areas of north-east brazil. | the objective of this study was to detect the pathways of transmission of ascaris lumbricoides eggs in low-income households of two brazilian cities. an inexpensive flotation technique for egg isolation was applied for the determination of soil contamination in 227 households. the median contamination levels were 0.8 (indoor), 0.3 (backyard) and 0.6 (defaecation site) eggs per gram soil. the contamination levels showed no significant correlation with the presence or quality of latrines or flush ... | 1992 | 1560490 |
| effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs. | in experimental ascariosis (ascaris suum) of pigs a preparation of glucan combined with pig immunoglobulin and zinc was found to significantly elevate the levels of both t and b lymphocytes in the blood, and to significantly stimulate the phagocytic ability of monocytes and neutrophilic granulocytes in experimental pigs. the protective effect of the preparation, assessed by the reduction in the number of migrating ascarid larvae in the lungs of pigs was 65%. | 1992 | 1561757 |
| characterization of cdna clones for the alpha subunit of pyruvate dehydrogenase from ascaris suum. | the pyruvate dehydrogenase complex of the adult parasitic nematode ascaris suum functions in the reducing environment present in their anaerobic mitochondria. these organelles use fumarate and enoyl coas as terminal electron acceptors instead of oxygen. a lambda gt11 cdna library was constructed from rna isolated from adult ascarid muscle. partial clones for the pyruvate dehydrogenase alpha subunit were isolated by screening the lambda gt11 library with a specific antiserum. full-length clones ( ... | 1992 | 1565136 |
| purification and characterization of polyamine oxidase from ascaris suum. | the interconversion of polyamines in the parasite nematode ascaris suum by a novel type of polyamine oxidase was demonstrated. the nematode enzyme was clearly distinguishable from monoamine and diamine oxidases as well as from the mammalian polyamine oxidase, as shown by the use of the specific inhibitors pargyline, aminoguanidine and mdl 72527 respectively. all three inhibitors had no effect on the parasite polyamine oxidase, and the enzyme did not accept diamines such as putrescine, cadaverine ... | 1992 | 1567380 |
| fatal ascaris pancreatitis: an uncommon problem in the west. | gastrointestinal parasites, although an uncommon cause of biliary and pancreatic problems in western medicine, can produce fatal complications. a swiss patient presented with acute pancreatitis, with no evidence of gall stones or history of alcohol abuse. he died after a short fulminant illness. at necropsy, an ascaris lumbracoides was found impacted within the ampulla of vater and serves as a reminder that non-indigenous causes of biliary tract obstruction should not be overlooked. | 1992 | 1568664 |
| the onset and recovery from airway hyperresponsiveness: relationship with inflammatory cell infiltrates and release of cytotoxic granule proteins. | previous studies from our laboratory have demonstrated a temporal relationship between eosinophil influx into the airways and the onset of airway hyperresponsiveness to inhaled methacholine. the purpose of the present study was to extend this observation by evaluating changes in airway cellular composition and measuring the levels of granulocyte-derived mediators recovered in bal fluid during the onset and recovery from antigen-induced airway hyperresponsiveness. airway cellular composition, air ... | 1992 | 1571822 |
| purification of glutathione reductase from muscle of the adult parasitic nematode ascaris suum. | | 1992 | 1574090 |
| protein chromatographic study on adult ascaris lumbricoides, ascaris vitulorum and toxocara canis. | chromatographic analysis using sephacryl s-300 was done on protein extract of adult ascaris lumbricoides, a. vitulorum and toxocara canis worms. six fractions were separated from both ascaris species and nine from t. canis. fractions revealed a variety of molecular weights in relation to the three species of worms that ranged from 6,000-70,000 daltons. crude extract of t. canis showed cross reaction with both species of ascaris sera using double gel diffusion, while no reaction was obtained usin ... | 1992 | 1578165 |
| indirect fluorescent antibody test in diagnosis of acute fasciolitic syndrome. | 250 patients with fever, hepatomegaly and eosinophilia were investigated for parasitic infection by stool, urine, thick blood smear examination and sero-diagnosis (elisa for anti-toxocara and ifa for anti-fasciola). those with patent parasitic infections (41) and anti-toxocara seropositivity (10) were excluded from follow up. the other were 3 months followed up. the detected infections were 10 ascaris, 15 strongyloides, 20 toxocara, and 25 schistosoma mansoni. other 30 were positive for fasciola ... | 1992 | 1578173 |
| isolation of ascaris suum components which suppress ige antibody responses. | an extract from adult ascaris suum was fractionated on a sephadex g-200 column and individual elution fractions assessed for their ability to induce or suppress an ige antibody response in mice immunized simultaneously with a heterologous antigen (ovalbumin). the results showed that, whilst fractions eluted in the first peak suppressed the anti-ovalbumin ige antibody production, those eluted in the third peak induced a significant anti-ascaris ige response. the mixture of the two kinds of fracti ... | 1992 | 1582696 |
| ige-mediated rhesus monkey asthma: natural history and individual animal variation. | this report reviews the 20 years of experience with naturally occurring allergy in dogs and allergic rhesus monkeys. the primate model of allergy is characterized by ige-mediated cutaneous and respiratory responses to ascaris antigen. individual assessments of these monkeys have been carried out over several years and separate populations which are analogous to human ige-mediated allergic populations defined. these populations are as follows: (1) monkeys which have persistent and consistent ige- ... | 1992 | 1582707 |
| vascular permeability and airway narrowing during late asthmatic response in dogs treated with metopirone. | recently, we have developed an animal model of late asthmatic response (lar) by treating naturally sensitized dogs to ascaris suum antigen with the cortisol-synthesizing inhibitor, metopirone. by using this animal model, we examined the contribution of edema in the airway wall to the development of lar. to study whether airway microvascular leakage is increased in association with lar, we performed antigen challenge in dogs treated with metopirone. we measured the amount of extravasated evans bl ... | 1992 | 1583251 |
| polar zipper sequence in the high-affinity hemoglobin of ascaris suum: amino acid sequence and structural interpretation. | the extracellular hemoglobin of ascaris has an extremely high oxygen affinity (p50 = 0.004 mmhg). it consists of eight identical subunits of molecular weight 40,600. their sequence, determined by protein chemistry, shows two tandemly linked globin-like sequences and an 18-residue c-terminal extension. two n-linked glycosylation sites contain equal ratios of mannose/glucosamine/fucose of 3:2:1. electron micrographs suggest that the eight subunits form a polyhedron of point symmetry d4, or 42. the ... | 1992 | 1584800 |
| efficacy of an in-feed preparation of ivermectin against endoparasites and scabies mites in swine. | in 2 trials, the efficacy of an in-feed preparation of ivermectin was evaluated in 40 pigs naturally infected with endoparasites and sarcoptes scabiei var suis. treated pigs (n = 10 in each trial) were fed a ration containing 2 ppm ivermectin for 7 days, followed by consumption of a nonmedicated ration for the remainder of the trial. control pigs (n = 10 in each trial) were fed a complete, nonmedicated ration for the duration of the trial. pigs in trial a were monitored for 14 days after treatme ... | 1992 | 1586019 |
| surgical manifestations of ascariasis in childhood. | amongst 50 patients with surgical complications of ascaris lumbricoides 35 patients (70%) were treated conservatively and 15 patients (30%) required surgical intervention which included squeezing of worms to distal colon, resection and anastomosis of ileum, appendicectomy and peritoneal lavage. intestinal perforation was observed in 3 cases. two patients (4%) died because of postoperative complications. those presenting with subacute intestinal obstruction had no mortality or morbidity. although ... | 1992 | 1588117 |
| [the prevalence of intestinal parasites in primary school students in trabzon and the importance of education of parasite patients]. | hundred and forty stool samples obtained from fourth classes (10 years of age) of the primary school students were examined for intestinal parasites. in addition, effect of education and variety of education on parasitic diseases were investigated. of the 140 students 65% were found to be positive for intestinal parasites. ascaris lumbricoides 15.7%, trichuris trichiura 22.85%, giardia intestinalis 7.14%, ascaris lumbricoides + trichuris trichiura 15.7%, giardia intestinalis + trichuris trichiur ... | 1992 | 1588851 |
| the effect of repeated chemotherapy on age-related predisposition to ascaris lumbricoides and trichuris trichiura. | this study examines the persistence of predisposition to ascaris lumbricoides and trichuris trichiura during repeated chemotherapy in an urban community in kuala lumpur, malaysia. significant predisposition was observed over 2 periods of reinfection with and without age-standardization of data. analysis of different age groups indicated that predisposition was most strongly detectable in the younger age classes. the intensities of infection with both parasites were strongly correlated at each cy ... | 1992 | 1594301 |
| effects of cholinergic agents on the metabolism of choline in muscle from ascaris suum. | the incorporation of [methyl-14c]choline into the choline-containing compounds of ascaris suum muscle and the effects of acetylcholine and its agonists, carbachol and levamisole, on this incorporation were studied. previous experiments reported a stimulation of phosphatidylcholine (lecithin) metabolism upon the administration of acetylcholine. acetylcholine administered in vitro to a. suum muscle and body wall preparations resulted in a stimulation of phospholipase c activity that, in turn, prod ... | 1992 | 1597777 |
| child growth and schistosomiasis japonica in northeastern leyte, the philippines: cross-sectional results. | the association between schistosomiasis japonica and child growth was studied cross-sectionally in 1,561 males and females aged 4-19.9 years residing in an endemic region of northeastern leyte, the philippines. stature, weight, upper arm muscle area, and sum of triceps and subscapular skinfold thicknesses were measured and related to presence of schistosoma japonicum eggs in kato stool smears and to the intensity of infection assessed by quantitative egg count. the presence of hookworm, ascaris, ... | 1992 | 1599051 |
| a new approach to morbidity risk assessment in hookworm endemic communities. | the relationship between paired hookworm prevalence and mean intensity of infection data from geographically defined communities was examined. the results show that, in spite of major socio-economic and environmental differences between communities, the relationship is consistent and non-linear. a generalized value of k (the exponent of the negative binomial distribution) for hookworms was estimated to be 0.34, which is consonant with previous estimates from cross-sectional data. maximum likelih ... | 1992 | 1601081 |
| [liver abscesses due to ascaris lumbricoides]. | | 1992 | 1602887 |
| [ascaris suum infection as a cause of clinical problems in cattle?]. | a sudden decrease in milk yield, increased respiratory rate and occasional coughing were observed in dairy cows on two farms in spring 1991. pigs were also kept on these farms, and pastures grazed by the cattle had been fertilised with pig slurry. laboratory investigations of some of the cattle showed eosinophilia and high elisa titres of antibodies against ascaris suum. on one farm the clinical symptoms disappeared after the animals had been treated with oxfendazole and on the other farm the sy ... | 1992 | 1604447 |
| immunoepidemiology of ascaris lumbricoides: relationships between antibody specificities, exposure and infection in a human community. | the serum antibody responses of 124 people naturally exposed to ascaris lumbricoides infection were analysed by immunoprecipitation of radio-isotope labelled 3rd- and 4th-stage larval ascaris suum excretory and secretory antigens (l3/4 es). profiles of antigens recognized were visualized by polyacrylamide gel electrophoresis (sds-page), and the band intensities of the 12 major precipitated antigens were individually scored. most subjects were seropositive, but considerable variation was observed ... | 1992 | 1614731 |
| an unusual cause of nasogastric tube obstruction. | | 1992 | 1616147 |
| the gmp reductase gene of the nematode ascaris lumbricoides var. suum. | | 1992 | 1620164 |
| the two main rdna size classes of ascaris lumbricoides: comparison of transcription termination and spacer organization. | structural and functional analyses were carried out to compare transcription termination and intergenic spacer organization between the two heterogeneous ribosomal dna size classes of ascaris lumbricoides. by performing mung bean nuclease mapping in vivo, we localized the 3' end of the mature 26s rrna to the same position in both forms. this site coincides with the in vivo and in vitro transcription termination site of the 40s-precursor rrna from both rdna size classes. we demonstrate that the 3 ... | 1992 | 1620592 |
| mechanistic studies in the transcuticular delivery of antiparasitic drugs. ii: ex vivo/in vitro correlation of solute transport by ascaris suum. | using live, intact ascaris suum and a closed perfusion system, the absorption kinetics and tissue distribution of selected radiolabeled permeants were measured to determine the importance of the transcuticular pathway for drug absorption. the data support the conclusions established by previous in vitro transport studies which utilized excised cuticle-hypocuticle tissue preparations. the external surface of a. suum can be breached by drugs and the rate-determining barrier is the lipoidal hypocut ... | 1992 | 1625697 |
| dna synthesis in the early embryo of the nematode ascaris suum. | we have used microspectrofluorimetry to measure the rate of dna synthesis in the first two embryonic cell cycles of the parasitic nematode ascaris suum. the s-phase of the early ascaris cell cycles occupies at most 1 hr; g2 phase is prominent and occupies approximately 11 hr; no g1 phase could be detected. these results contrast with our previous measurements made with embryos of the free-living nematode caenorhabditis elegans, in which the earliest cell cycles consist of simple alternations bet ... | 1992 | 1628758 |
| abdominal complications of ascaris lumbricoides in children. | | 1992 | 1630003 |
| [hepatobiliary ascaridiasis in childhood: echographic diagnosis]. | ascaris lumbricoides infestation in the liver and biliary tract causes various clinical manifestations due to the migration of larvae and adult organisms. parasites in the bile ducts cause obstruction and such complications as cholecystitis, cholangitis and liver abscesses. we report the us findings of biliary ascaridiasis as observed in a series of 13 children over a 3-year period. the patients (6 females and 7 males) presented hepatosplenomegaly, abdominal distension, right subcostal pain; 3 o ... | 1992 | 1631339 |
| [immunostimulatory effects of certain substances in experimental ascaridiasis in pigs]. | the effects were studied of four immunomodulative substances of biological origin (purified glucan, glucan with porcine immunoglobulin and zinc, transfer factor and vitamin a) on t and b lymphocytes, along with their protective effects in pigs with experimental ascariasis. these substances activated t and b lymphocytes with different intensity. the nonspecific stimulation of these cells in the blood of pigs with the immunomodulative substances intensified the protective mechanisms; this resulted ... | 1992 | 1632051 |
| changes in cell-mediated immune responses after experimentally-induced anaphylaxis in dogs. | experimentally-induced type 1 hypersensitivities were induced in normal dogs to either ovalbumin or ascaris antigen. in vitro and in vivo cell-mediated immune responses were measured before sensitization and again at 1 and 6 days after induction of anaphylaxis by intravenous challenge with antigen. histamine-modulated lymphocyte functions, such as histamine-induced suppression, histamine co-mitogen induced blastogenesis and the in vivo cutaneous responses to intradermally injected mitogens decre ... | 1992 | 1632068 |
| intestinal parasite infection in the kampuchean refugee population 6 years after resettlement in canada. | a follow-up prevalence study was done in 1989 of the same kampuchean refugee population (247 subjects) that had been screened and treated for intestinal parasite infection 6 years earlier. a control group (102 subjects) included kampuchean refugees who had arrived in montreal at about the same time. these groups did not differ in age, sex, family size, or number of months spent in refugee camps. statistically significant prevalence differences were observed in the rescreened group between 1982-1 ... | 1992 | 1634813 |
| the pattern of human intestinal helminth infections in farming communities in different parts of ondo state, nigeria. | faecal samples collected from people inhabiting project areas of owena river basin and rural development authority at okitipupa, akure ifon and ajowa which are located in different geographical areas of ondo state, nigeria, were examined for helminth ova and larvae. the common worms were ascaris lumbricoides, trichuris trichiura and hookworms with average occurrences of 43.1% 23.4% and 17.0% respectively. infections were highest in subjects in the age groups of 6-10 and 11-15 years and lowest in ... | 1992 | 1637737 |
| ascaris suum: are trypsin inhibitors involved in species specificity of ascarid nematodes? | inhibitors of porcine trypsin were prepared from aqueous extracts of the parasitic nematodes ascaris suum (hogs) and ascaris lumbricoides (human). in this study three experiments were performed. (1) polyclonal antibodies were prepared against one isoform of trypsin inhibitor from each parasitic nematode. each antibody reacted with all isoforms from itself as well as all isoforms from the other parasite. (2) association equilibrium constants were measured by titrating host trypsins (porcine or hu ... | 1992 | 1639157 |
| cross-reactions of sera from toxocara canis-infected mice with toxascaris leonina and ascaris suum antigens. | the elisa method using larval excretory-secretory (e/s) products and homogenized toxocara canis, toxascaris leonina and ascaris suum adult worm extract were used to determine possible cross-reactions in balb/c and c57bl/10 mice, inoculated with embryonated eggs or adult worm extract of t. canis in single and multiple doses. when we used sera of mice infected with embryonated eggs of t. canis against different heterologous antigens, we observed no cross-reactions in balb/c mice against a. suum e/ ... | 1992 | 1639565 |
| crystallization of the nad-dependent malic enzyme from the parasitic nematode ascaris suum. | the malic enzyme from muscle mitochondria of the parasitic nematode ascaris suum is a tetramer of 65 kda monomers that catalyzes the oxidative decarboxylation of malate to pyruvate and co2 with nad cofactor as oxidant. this malic enzyme is critical to the nematode for muscle function under anaerobic conditions. unlike mammalian versions of the enzyme such as that found in rat liver, which require nadp as cofactor, the nematode version is an nad-dependent enzyme. we report the crystallization of ... | 1992 | 1640469 |
| moderate to heavy infections of trichuris trichiura affect cognitive function in jamaican school children. | a double-blind placebo trial was conducted to determine the effect of moderate to high loads of trichuris trichiura (whipworm) infection on the cognitive functions of 159 school children (age 9-12 years) in jamaica. infected children were randomly assigned to treatment or placebo groups. a third group of randomly selected uninfected children were assigned to a control for comparative purposes. the improvement in cognitive function was evaluated using a stepwise multiple linear regression, design ... | 1992 | 1641252 |
| on the distribution of a fluorescent ivermectin probe (4" 5,7 dimethyl-bodipy proprionylivermectin) in ascaris membranes. | the distribution of the fluorescent ivermectin derivative (4" 5,7 dimethyl bodipy proprionylivermectin, referred to here as bodipy ivermectin) was studied in muscle vesicle membranes from the parasite ascaris suum. incubation in bodipy ivermectin, at concentrations greater than 1 ng/ml, produced a dose-dependent fluorescence in the vesicles. the level of fluorescence was not depressed (but increased) by prior incubation with 1 microm unlabelled ivermectin. this observation suggested that most of ... | 1992 | 1641253 |
| morphology of the uterus of ascaris lumbricoides in the region where fertilization and formation of egg-shell occur. | morphology of the female reproductive system of ascaris lumbricoides l. was studied in the region starting with the junction between the oviduct and the uterus (o-u) up to the junction of both uterine branches into the vagina with regard to the process of fertilization and formation of egg-shells. in the o-u junction morphology differed in two following sections: a continuous simple squamous up to simple cuboidal epithelium, and simple cuboidal up to columnar epithelium with broad intercellular ... | 1992 | 1644348 |
| on the mechanism of penetration of ovicidal fungi through egg-shells of parasitic nematodes. decomposition of chitinous and ascaroside layers. | the decomposition of egg-shells of ascaris lumbricoides l. was studied microscopically using topochemical methods in a set of 32 strains of soil ovicidal fungi. it was found that even fungi displaying minimal chitinolytic activity in tests on purified chitin in vitro are able to dissolve chitin of egg-shells during the attack on live eggs. fungi without any chitinolytic activity penetrate probably only the mechanically damaged eggs. none of the studied fungi was capable of degrading enzymaticall ... | 1992 | 1644351 |
| helminth eggs in the sludge from three sewage treatment plants in czechoslovakia. | developmental stages of 8 species of parasitic helminths were found during examination of sludge from 3 sewage treatment plants in central bohemia, czechoslovakia. the eggs were comprised from ascaris sp., toxocara sp., toxascaris leonina, parascaris equorum, enterobius vermicularis, trichuris sp., capillaria sp. and hymenolepis spp. the quantity of these organisms in 100 g of dried sludge ranged between 24-105 eggs of ascaris sp., 12-47 eggs of toxocara sp., 0-8 eggs of toxascaris leonina, 0-1 ... | 1992 | 1644362 |
| the physiology and pharmacology of neuromuscular transmission in the nematode parasite, ascaris suum. | the organization of ascaris motoneurones and nervous system is summarized. there is an anterior nerve ring and associated ganglia, main dorsal and ventral nerve cords which run longitudinally, and a small set of posterior ganglia. cell bodies of motoneurones are found in the ventral nerve cord and occur in 5 repeating 'segments'; each contains 11 motoneurones. seven morphological types of excitatory or inhibitory motoneurone are recognized. each ascaris somatic muscle cell is composed of the con ... | 1991 | 1647516 |
| antigen-induced mediator release in primates. | we examined the release of bronchoactive mediators into the airways of allergic primates during the acute response to specific antigen inhalation. twelve adult male cynomolgus monkeys (macaca fascicularis) with a naturally occurring respiratory sensitivity to inhaled ascaris suum extract were anesthetized and intubated for each study. respiratory system resistance (rrs) and dynamic lung compliance (cldyn) were measured before and after antigen inhalation, and the release of mediators into the ai ... | 1991 | 1648317 |
| characterization of sulfidopeptide leukotriene responses in sheep tracheal smooth muscle in vitro. | we have investigated the effects of leukotrienes (lts) on isolated tracheal smooth muscle from sheep sensitive to ascaris suum antigen. ltc4 and ltd4 produced dose-dependent contractions of sheep trachea, but lte4 was virtually inactive. ym-17690, a non-analogous lt agonist, produced no contractile response up to 100 microm. indomethacin (5 microm) had no effect on ltc4- and ltd4-induced contractions. l-serine borate (45 mm), an inhibitor of gamma-glutamyl transpeptidase, shifted the dose-respon ... | 1991 | 1655181 |
| antagonist properties of arylaminopyridazine gaba derivatives at the ascaris muscle gaba receptor. | 1. in a previous study, it was shown that the potency order for two arylamino-pyridazine derivatives, sr95531 and sr95103, was different in ascaris suum when compared to vertebrate preparations. sr95531, the most potent analogue at the vertebrate gabaa receptor, was found to be very weak at antagonizing gaba responses in ascaris, but sr95103, approximately 20 times less potent than sr95531 in vertebrate preparations, was more potent than sr95531 in ascaris. these results suggested the existence ... | 1991 | 1658181 |
| novel regulation of pyruvate dehydrogenase phosphatase activity from mitochondria of the parasitic nematode, ascaris suum. | | 1991 | 1664050 |
| ipd-1151t: a prototype drug for ige antibody synthesis modulation. | ipd-1151t [(+/-)-[2-[4-(3-ethoxy-2-hydroxypropoxy)phenylcarbamoyl]-ethyl] dimethylsulfonium p-toluenesulfonate] inhibits not only antigen-induced histamine release from mast cells but also ige antibody formation. the present paper describes the inhibitory effect of ipd-1151t on the ige antibody formation. the ige antibody formation in balb/c mice which had been immunized with dinitrophenylated ascaris extract (dnp.as) plus alum was inhibited dose-dependently by ipd-1151t given p.o. the formation ... | 1991 | 1665310 |
| effect of nedocromil sodium on allergen-, paf-, histamine- and bradykinin-induced airways vasodilatation and pulmonary obstruction in the pig. | 1. the influence of nedocromil sodium on the nasal and bronchial effects induced by allergen, platelet-activating factor (paf), capsaicin, histamine and bradykinin aerosol challenge in ascaris-sensitized and pentobarbitone-anaesthetized pigs was studied. blood flow changes in the bronchial and nasal circulation were measured with ultrasonic flow probes around the supplying arteries, and vascular resistance was calculated. changes in pulmonary resistance (rpulm), dynamic compliance (cdyn), mean a ... | 1991 | 1665736 |
| propionyl-coa condensing enzyme from ascaris muscle mitochondria. ii. coenzyme a modulation. | the propionyl-coa condensing enzyme which catalyzes the first step in the biosynthesis of 2-methylbutyrate and 2-methylvalerate by ascaris muscle appears to exist in at least three forms in the mitochondria of this parasitic nematode. two forms, a and b, were separated by ion exchange chromatography on cm-sephadex. chromatography on phosphocellulose resulted in the recovery of one minor peak (i) and two major peaks with activity (ii and iii). a and b as well as i, ii, and iii differed in their s ... | 1991 | 1671327 |
| intestinal parasitic infections in schoolchildren of abha (asir), saudi arabia. | stool specimens from 1282 children between the age of 5 and 13 years attending 10 primary schools for boys in the city of abha, southwestern saudi arabia, were examined for the presence of intestinal parasites. of these, 313, (24.4%) were found infected with one or more species of 11 intestinal protozoa and helminths. the most common pathogenic protozoa being giardia lamblia (10.9%) followed by entamoeba histolytica (4.1%). the nonpathogenic protozoan, entamoeba coli had the highest prevalence r ... | 1991 | 1671621 |
| patterns of hookworm and ascaris infection in dar es salaam. | | 1991 | 1671626 |
| hallucinogenic and neuroleptic drug interactions with potential neurotransmitter receptors in parasitic nematodes. | receptors potentially involved in neurotransmitting have been characterised in the muscle tissue and in whole worms of the nematodes ascaris suum and onchocerca volvulus, respectively. binding studies revealed a high affinity for lsd with apparent kd values of 94 nm for a. suum and 120 nm for o. volvulus, whereas those of the neuroleptics haloperidol, spiperone and mianserin were found to be in the micromolar range. a variety of neurotransmitter antagonists, known to bind with high affinities ei ... | 1991 | 1674821 |
| effect of ascaris lumbricoides infection on brain enzymes. | | 1991 | 1681943 |
| the efficacy of treatment with albendazole. | albendazole, a benzimidazole derivative, was administered as a single dose of either 400 mg or 600 mg to two groups to ascertain the efficacy, tolerance and safety of the regimens. at a dose of 400 mg, a cure rate 35/36 (97%) against ascaris was found. at 600 mg, the cure rate was 21/30 (70%), significantly lower than the 400 mg rate. against trichuris, albendazole at 400 mg had a cure rate of 21/48 (44%), at 600 mg there was a cure rate of 29/43 (67%). mild side effects were noted in 7 individu ... | 1991 | 1685866 |
| effects of plumbagin on development of the parasitic nematodes haemonchus contortus and ascaris suum. | 1. plumbagin (5-hydroxy,2-methyl-1,4-napthoquinone) inhibited the motility and survival of haemonchus contortus first-stage larvae (l1) with an ed50 of 1 microgram/ml, but was less effective in preventing the development of h. contortus to infective third-stage larvae in a faecal slurry assay. 2. of the structural analogs tested, plumbagin was the most potent in preventing development of l1 followed in decreasing order of potency by 1,4-naphthoquinone, 5-hydroxy-1,4-napthoquinone (juglone) and 1 ... | 1991 | 1687553 |
| effects of the new antiallergic drug epinastine and ketotifen on repeated antigen challenge-induced airway hyperresponsiveness in rats. | airway hyperresponsiveness (ahr) is a critical component in bronchial asthma, probably associated with airway inflammation. the effects of epinastine (wal-801 cl, cas 80012-43-7), a new antiallergic drug, and ketotifen on ahr were investigated with a new ahr model in rats. male wistar rats which were actively sensitized with dnp-ascaris (dnp-asc) antigen, were challenged by inhaling dnp-asc 3 times every 48 h. the airway responsiveness to inhaled ach was determined 24 h after the last antigen ch ... | 1991 | 1687721 |
| potent inhibitory activity of hsr-6071, a new antiallergic agent, on passive cutaneous anaphylaxis (pca). | antiallergic effects of 6-(1-pyrrolidinyl)-n-(1h-tetrazol-5-yl)-2-pyrazinecarboxamide (hsr-6071), a newly synthesized agent, were investigated. the 48-hr homologous passive cutaneous anaphylaxis (pca) in rats was inhibited in a dose-dependent manner by i.v. and p.o. administration of the agent (ed50 = 0.0096 mg/kg and 0.18 mg/kg, respectively). the ige-mediated histamine release from rat peritoneal exudate cells was inhibited by hsr-6071, with an ic50 of 4.6 x 10(-10) m. regarding the non-immuno ... | 1990 | 1689783 |
| identification of the major ascaris allergen and its purification to homogeneity by high-performance liquid chromatography. | the major allergen from the body fluid of adult ascaris suum (abf) has been identified and purified to homogeneity using gel permeation high-performance liquid chromatography (hplc). the purity of the ascaris body fluid allergen (aba-1) was confirmed by hplc and sds-page. aba-1 appears to be a 25-kda dimer in its native form, which runs as a 10-kda monomer on sds-page under reducing conditions. the allergenicity of the hplc-purified protein was confirmed using isolated in vivo-sensitised mast ce ... | 1990 | 1690856 |
| studies on antiallergic agents. i. synthesis and antiallergic activity of novel pyrazine derivatives. | various pyrazine derivatives were synthesized and their antiallergic activity was examined. the inhibitory activity on allergic histamine release of the compounds bearing a 5-tetrazolyl group was more potent than that of the corresponding carboxyl derivatives. the introduction of -conh- or -nhco- between the pyrazine ring and the 5-tetrazolyl group as a spacer greatly enhanced the activity. n-(1h-tetrazol-5-yl)-2-pyrazinecarboxamide (i-3) was estimated to exhibit nearly the same potency as disod ... | 1990 | 1692517 |
| surface properties of membrane vesicles prepared from muscle cells of ascaris suum. | to facilitate biochemical, pharmacological, and biophysical studies on the membrane of the body muscle of ascaris suum, a method for preparing intact vesicles was developed. vesicles were prepared by incubating a muscle flap preparation with 1 mg/ml collagenase in a saline solution and then washing in saline without enzyme. the vesicles then formed gradually over the next hour as outgrowths of the original surface membrane from the bag region of the muscle. the vesicles were harvested readily by ... | 1990 | 1693673 |
| role of ascariasis in surgical abdominal emergencies in the rangoon children's hospital, burma. | patients aged 1 month to 12 years admitted with an acute abdominal surgical condition comprising 226 cases with and 206 cases without ascariasis, recorded in the operation theatre registers and in-patients clinical sheets, were studied in relation to morbidity, duration of operation and hospital stay and mortality. annually, 7.5% of laparotomies were due to complications of ascariasis. operations for ascariasis accounted for 10.6% of all hospital admissions for an acute abdominal emergency. also ... | 1990 | 1694645 |
| [antigenic preparations from opisthorchis and their use in immunodiagnosis. 1. the specificity of antigenic preparations from opisthorchis]. | immunochemical tests of various antigenic preparations derived from opisthorchis revealed specific components in metabolic, somatic and ovular specimens. furthermore, the study revealed a common somatic antigenic components in opisthorchis, echinococcus and fasciola. the specificity revealed makes the implementation of opisthorchis immunoreagents advisable for the diagnosis of opisthorchiasis. | 1990 | 1695991 |
| rhesus monkey airway responses to substance p. | the tachykinin, substance p (sp), was used as a model to study airway and cutaneous responses in a group of normal rhesus monkeys or animals with ige-mediated cutaneous reactions to ascaris antigen (aa) alone or animals with both ige-mediated cutaneous and airway responses to aa. aerosolized sp (10 mg/ml) resulted in airway responses qualitatively similar in duration and pulmonary function abnormalities to aa. these sp airway responses were most closely associated with the presence of ige antibo ... | 1990 | 1698721 |
| characterization of monoclonal antibodies to young-adult worms of angiostrongylus cantonensis. | three igg1 and one igg2a monoclonal antibodies were obtained by fusion of ns-1 cells with spleen cells of balb/c mice immunized with young-adult worm antigen of a. cantonensis. these 4 monoclonal antibodies were specific to a. cantonensis without cross-reactivity to any antigens of toxocara canis, ascaris suum, clonorchis sinensis, dirofilaria immitis, paragonimus westermani, gnathostoma spinigerum, strongyloides stercoralis and anisakis spp. detected by elisa. the affinity constants of monoclon ... | 1990 | 1701759 |
| a patch-clamp study of acetylcholine-activated ion channels in ascaris suum muscle. | acetylcholine-activated single-channel currents were recorded from cell-attached and inside-out patches of isolated muscle vesicles from ascaris suum. acetylcholine (1-10 mumols l-1) activated cation-selective channels of two amplitudes: 40-50 ps and 25-35 ps. both channels had linear i/v relationships and mean open durations independent of voltage. the larger conductance was analysed in detail to determine its open-, closed- and burst-time kinetics; the open and burst durations were composed of ... | 1990 | 1703557 |
| airway effects of inhaled bradykinin, substance p, and neurokinin a in sheep. | the effects of inhaled bradykinin (bk), substance p (sp), and neurokinin a (nka) on pulmonary resistance and airway responsiveness to carbachol were studied in conscious allergic sheep. inhaled bk (20 breaths, 0.1 to 5.0 mg.ml-1) caused dose-dependent increases in pulmonary resistance. neither inhaled sp nor nka (20 breaths, 0.1 to 1.0 mg.ml-1) produced significant bronchoconstriction in allergic sheep. however, the response to sp could be enhanced (p less than 0.05) by pretreatment with the neu ... | 1991 | 1704388 |
| anti-allergic effects of ketanserin on animal models of allergic reactions. | the aim of the present study is to investigate the effect of ketanserin, a potent and selective s2-serotonergic antagonist, on some anaphylactic reactions in mice, rats and guinea-pigs. ketanserin (1 and 5 mg/kg) inhibited both ige antibody-mediated 48 hr homologous passive cutaneous anaphylaxis and igg antibody-mediated 1.5 hr homologous passive cutaneous anaphylaxis in mice. in histamine-, serotonin- and ltc4-induced skin reactions in mice, ketanserin inhibited an increase of capillary permeab ... | 1990 | 1710442 |
| effect of puva radiation on anaphylactic histamine release from rat dermal tissues. | we have devised a new in vitro model of type i cutaneous anaphylaxis. male albino rats were sensitized with dnp-ascaris. abdominal skin was shaved, and thin, split-thickness slices of skin were cut with a dermatome. the dermis was excised and cut into 100 mg pieces. the dermal tissue was incubated with antigen in tyrode's solution for 30 min at 37 degrees c. antigen-induced histamine release from dermal tissue was measured fluorimetrically. using this system, we measured histamine release from p ... | 1991 | 1710861 |
| heterogeneity in the expression of surface-exposed epitopes among larvae of ascaris lumbricoides. | quantitative immunofluorescence was used to examine differences in the binding of antibody to the surfaces of individual living infective stage larvae of ascaris lumbricoides. using rabbit antisera, it was first established that larvae cultured for 48 h after artificial hatching were relatively uniform in their levels of antibody binding and in minimal exposure of epitopes expressed by later larval stages. aliquots from a pool of larvae were probed with serum from individual infected people livi ... | 1991 | 1711197 |
| association between histamine-containing mast cells and sensory nerves in the skin and airways of control and capsaicin-treated pigs. | the association between mast cells (visualized by routine staining and immunohistochemistry for histamine) and capsaicin-sensitive nerves (containing calcitonin gene-related peptide (cgrp) and substance p (sp] was studied in the pig. in the 1-ethyl-3(3-diethylaminopropyl)carbodiimide (edcdi)-fixed skin tissue, histamine-containing mast cells and cgrp/sp-positive nerves were found in close association around blood vessels. in the edcdi-fixed airway mucosa, only single histamine-containing mast ce ... | 1991 | 1714354 |
| the complete primary structure of a nematode alpha 2(iv) collagen and the partial structural organization of its gene. | we have isolated and characterized cdna and genomic dna clones which encode an alpha 2(iv) collagen chain from the parasitic nematode ascaris suum. in addition we have determined, by nucleic acid sequence analysis, the structural organization of approximately two-thirds of the gene. this analysis has shown that the gene contains at least 15 introns, and those that have been characterized range in size from 141 to 854 base pairs. the derived protein sequence contains 1763 amino acids and includes ... | 1991 | 1714907 |
| leukotrienes, thromboxane a2, and prostaglandins during systemic anaphylaxis in sheep. | we investigated the roles of eicosanoid mediators in acute systemic anaphylaxis in anesthetized sheep. sheep were sensitized with dinitrophenylated ascaris suum extract and were challenged with an intravenous injection of dinitrophenylated bovine serum albumin. during anaphylaxis, cyclooxygenase inhibitors eliminated the elevation of arterial plasma levels of thromboxane b2 and 6-ketoprostaglandin f 1 alpha but markedly elevated the levels of leukotriene e4 in lung lymph without significantly el ... | 1991 | 1716064 |
| studies on the ionic selectivity of the gaba-operated chloride channel on the somatic muscle bag cells of the parasitic nematode ascaris suum. | the reversal potential for the gaba-elicited hyperpolarization (egaba) of the muscle bag cells of the parasite nematode ascaris suum is near to the equilibrium potential for chloride ions (ecl) indicating that this is a chloride-mediated event. the value of egaba in the presence of different anions indicates the selectivity sequence of the gaba-operated chloride channel as scn- greater than br-, i- greater than clo3-, no3-, cl- greater than c2h5coo- greater than ch3coo-, hcoo-, ohch2ch2so3h-, ch ... | 1991 | 1716924 |
| an enzyme-linked immunosorbent assay for diagnostic detection of taenia saginata copro-antigens in humans. | an immunodiagnostic sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection of soluble taenia saginata antigens in stool samples (copro-antigens) of infected humans, using affinity-purified polyclonal antibodies obtained from rabbits hyperimmunized with excretory/secretory antigens derived from t. saginata maintained in vitro. investigation of operating characteristics showed very low cross-reactivity with crude antigens from helminths other than taenia, including dipy ... | 1991 | 1719664 |
| fatal respiratory obstruction due to ascaris lumbricoides--a case report. | | 1991 | 1719931 |
| effect of a novel antiallergic drug, pemirolast, on activation of rat peritoneal mast cells: inhibition of exocytotic response and membrane phospholipid turnover. | the effects of a potent antiallergic drug, pemirolast (tbx), on activation of signal-transducing phospholipase c and a2, were examined in rat peritoneal mast cells. tbx at concentrations between 10(-7) and 10(-5) g/ml effectively inhibited degranulation in response to both antigen and compound 48/80. at the same concentrations the drug markedly suppressed the formation of 1,2-diacylglycerol and phosphatidic acid, and the decrease in phosphatidylinositol, suggesting the prevention of phospholipas ... | 1991 | 1721611 |
| effects of ryo-kan-kyomi-sin-ge-nin-to extract on degranulation of and histamine release from rat mast cells. | the effects of ryo-kan-kyomi-sin-ge-nin-to (rksg)) extract, a medicinal agent traditionally used in china and japan for treatment of asthma, on the degranulation of and histamine release from rat mast cells were studied. at a concentration of 5 mg/ml rksg, degranulation of mast cells stimulated either by antigen (dnp-ascaris) or compound 48/80 was markedly suppressed. at a concentration of 1-5 mg/ml rksg, histamine release from mast cells due to application of either antigen or compound 48/80 wa ... | 1991 | 1722640 |
| larval exoantigens from ascarid nematodes are potent inducers of histamine release from human blood basophils. | histamine release (hr) from washed human blood cells after challenge with the excretory-secretory antigens (es) of the parasitic nematodes toxocara canis and ascaris suum was studied, employing a recently developed microfibre-based assay combined with hyperosmolar release media for maximal sensitivity. blood samples were obtained from 30 patients suspected of parasite infection and 11 healthy volunteers serving as controls. specific antibodies of ige, igg1 and igg4 subclasses were determined by ... | 1991 | 1723345 |
| structure of mitochondrial trna. | some mitochondrial (mt) trnas are presumed to have unusual secondary structures on the basis of dna sequence analyses. however there are few trnas whose structures have been investigated at rna level. thus we isolated some mt trnas to investigated their structures and also synthesized mt trna by run-off transcription. in these experiments, following results have been obtained. 1) bovine mt trna(ser)(agy) needs the interaction between the t-loop and d-arm for its aminoacylation activity. 2) the s ... | 1991 | 1726807 |
| dysfunction of nonadrenergic noncholinergic inhibitory system after antigen inhalation in actively sensitized cat airways. | we have investigated whether proteases released during antigen inhalation cause dysfunction of the nonadrenergic noncholinergic inhibitory nervous system (nancis). frequency-response (f-r) studies of nancis were performed before and after ascaris antigen (asc) inhalation using actively sensitized cats. nanc dilatatory effects were obtained by stimulating bilateral cervical vagi under cholinergic and beta-adrenergic blockade and serotonin-induced bronchoconstriction, and assessed by maximal perce ... | 1992 | 1731602 |
| molecular and biochemical aspects of nematode collagens. | collagens are major structural proteins of nematode cuticles and basement membranes (basal laminae). the collagen proteins that form these structures differ in their biochemical and physical properties and are encoded by distinct gene families. nematode basement membrane collagens are large proteins that show strong homology to basement membrane collagens of vertebrates. there appear to be 2 nonidentical basement membrane collagen genes in nematodes. cuticle collagens are about one-sixth the siz ... | 1992 | 1738051 |
| ascariasis mimicking an acute abdomen. | infection with the roundworm ascaris lumbricoides is common in children and may mimic an acute abdomen. the cases of two pediatric patients who presented to the emergency department with signs and symptoms of a surgical abdomen are presented to highlight the presentation of this infection. both cases were diagnosed by physical examination and radiographs and were treated successfully without surgical intervention. these cases illustrate the need for heightened awareness by the emergency physicia ... | 1992 | 1739217 |
| biochemical characterisation of the enzyme responsible for "activated l-serine sulphydrase" activity in nematodes. | the biochemical properties of the enzyme responsible for nematode "activated l-serine sulphydrase" activity (l-cysteine + r-sh----cysteine thioether + h2s) have been investigated using primarily the gastro-intestinal nematodes nippostrongylus brasiliensis and haemonchus contortus. the activated l-serine sulphydrase enzyme was found to be cytosolic in origin and exhibited maximal activity at ph 9.0. enzyme activity was widely distributed amongst the major tissues of adult female ascaris suum but ... | 1992 | 1740180 |
| in vitro damaging effect of leukocytes, serum, and peritoneal fluid on ascaris suum infective larvae. | peritoneal leukocytes, serum, and peritoneal fluid from ascaris suum-infected or noninfected mice were studied using 51cr release and larval mobility to determine if they had in vitro cytotoxicity for a. suum infective larvae. mouse serum or peritoneal fluid from infected and non-infected mice was added to leukocyte-larvae cultures to determine their effect on leukocyte damage to larvae. leukocytes from a. suum infected mice were cytotoxic for a. suum larvae in the presence of serum from infecte ... | 1991 | 1741924 |
| morphological and morphometric studies of the airways of sheep with acute airway hypersensitivity to inhaled ascaris suum. | the airways of 12 sheep with naturally-occurring allergic airway hypersensitivity, six of which had changes in both airway resistance and dynamic lung compliance (group a) and six of which had changes in only dynamic lung compliance (group b), were compared quantitatively with six non-reacting sheep (group c) in order to examine the relation between airway hypersensitivity and various morphological features thought to be related to airway hypersensitivity. compared to the non-reacting sheep (gro ... | 1991 | 1742207 |
| community control of ascaris lumbricoides in rural oyo state, nigeria: mass, targeted and selective treatment with levamisole. | a study to compare effects of mass, targeted and selective chemotherapy with levamisole (ketrax) as an action for the control of ascaris lumbricoides was carried out in three communities in rural oyo state, nigeria. selective treatment was applied in one village by treating the most heavily infected 20% of the inhabitants, targeted treatment in the second village involved children aged 2-15 years, while mass treatment was offered to all inhabitants excluding infants under 1 year and pregnant wom ... | 1991 | 1745554 |
| parasitic infections in young jamaicans in different ecological zones of the island. | data are presented for the first country wide prospective study on gastrointestinal tract parasitic infections done in jamaica. samples from 2,947 young jamaicans drawn from all ecological zones and from all parochial divisions of the island were analyzed. pica was practised by 8.6%. generally, prevalence of organisms was as follows: trichuris trichiura (12.3%), ascaris lumbricoides (9.5%), hookworm (2.2%), strongyloides stercoralis (0.3%), giardia lamblia (6.3%) and entamoeba coli (7.7%). sever ... | 1991 | 1750103 |
| [ascariasis: an unusual cause of cholangitis in our climate]. | | 1991 | 1752383 |
| lysophospholipase activity of ascaris suum-induced mouse peritoneal neutrophils and eosinophils. | lysophospholipase activity of mouse peritoneal neutrophils and eosinophils was studied to determine if neutrophils and eosinophils have lysophospholipase activity when treated with ascaris suum whole worm extract, if zymosan activated complement can induce increased lysophospholipase activity, or if the immune status of the host has an effect on lysophospholipase activity. neutrophils from noninfected or infected (immunized) mice were found to have increased lysophospholipase activity when treat ... | 1991 | 1753875 |
| antigenic cross-reactivity between necator americanus and ascaris lumbricoides in a community in papua new guinea infected predominantly with hookworm. | sero-epidemiological data are presented in which antigenic cross-reactivity between necator americanus and ascaris lumbricoides has been investigated in a community in papua new guinea infected predominantly with n. americanus. it is our contention that the antigenic cross-reactivity which undoubtedly exists between these species accounted for (i) a peak in antibody levels against n. americanus in 10-13 years old children (driven by infection with a. lumbricoides), and (ii) the maintenance of ap ... | 1991 | 1755061 |
| a controlled chemotherapeutic intervention trial on the relationship between ascaris lumbricoides infection and malnutrition in children. | a 2-year investigation, consisting of a controlled, non-blind, 3-monthly chemotherapeutic intervention trial, based on transmission dynamics of ascaris lumbricoides infection, was undertaken to prove or refute the role of a. lumbricoides as a contributor to childhood malnutrition. the study involved 1206 children aged 2-12 years in 21 villages in myanmar [burma] starting in august 1984. the intervention and non-intervention villages were comparable regarding almost all the important baseline var ... | 1991 | 1755063 |
| gastrointestinal parasitic infection in healthy jamaican carriers of htlv-i. | a subsample (1.6%; n = 13,260) of a healthy jamaican population of food-handlers, studied by murphy et al. (1991), who were serologically positive (n = 99) or negative (n = 113) for htlv-i was investigated for intestinal parasitic infection using coprological methods. helminth infection included ascaris lumbricoides (2.8%), trichuris trichiura (7.1%) and hookworms (6.1%). entamoeba coli was found in 21.8% of samples, while e. hartmanni, giardia lamblia, endolimax nana, iodamoeba bütschlii and ch ... | 1991 | 1758014 |
| phospholipids and protein kinase c in acetylcholine-dependent signal transduction in ascaris suum. | quantitatively, the major phospholipid in the muscle of the nematode ascaris suum was found to be phosphatidylcholine (lecithin). stimulation of ascaris muscle with acetylcholine or the agonists carbachol and levamisole increased the level of phosphorylcholine, 1,2-diacylglycerides and phosphatidic acid. increased levels of these compounds, together with the demonstration of phospholipase c activity, suggest that phospholipid hydrolysis may be associated with the ach response of the muscle via s ... | 1991 | 1762627 |
| structure of the polypeptide chain of extracellular hemoglobin from the nematode ascaris suum. | 1. ascaris suum extracellular hemoglobin is composed of eight identical single polypeptide chain subunits carrying two heme binding sites each. 2. limited trypsinolysis followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis gave a major band corresponding to half the molecular mass of an intact subunit. 3. peptide mapping of tryptic hydrolysates yielded 27 to 30 fluorescamine positive spots, about half the number of lysyl and arginyl residues in a polypeptide chain. 4. the findin ... | 1991 | 1764923 |