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freeze-etch characterization of the teguments of three metacestodes: echinococcus granulosus, taenia crassiceps, and taenia taeniaeformis.the objective of this study was to characterize the teguments of metacestodes of echinococcus granulosus, taenia crassiceps, and taenia taeniaeformis using the freeze-etch technique. metacestodes of e. granulosus (19 mo old), t. crassiceps (28 days old), and t. taeniaeformis (34 days old) from gerbils, mice and rats, respectively, were fixed for 2 hr in 3% glutaraldehyde and then prepared for freeze-etching and thin sectioning by standard techniques. freeze-etch replicas of the teguments of all ...19836685176
schistosoma mansoni, s. haematobium, and s. japonicum: identification of genus-, species-, and gender-specific antigenic worm glycoproteins.immunoreactive glycoproteins of schistosoma mansoni, s. haematobium, and s. japonicum adult worms which are genus-, species-, and gender-specific, or react with sera of patients infected with other parasites, have been identified. schistosomes were metabolically labeled with [35s]methionine and the concanavalin-a binding glycoproteins that were immunoprecipitated with sera of patients infected with one of three species of schistosoma or trichinella spiralis, taenia solium, echinococcus granulosu ...19846692879
long term follow-up of human hydatid disease (echinococcus granulosus) treated with a high-dose mebendazole regimen.fifteen patients with inoperable hydatid disease (echinococcus granulosus) were treated with an initial 6-week high-dose mebendazole regimen with a follow-up ranging from 3-7 years. ten of 15 patients showed both objective and clinical improvement, although two of these 10 relapsed 1-6 years after completing therapy. simple, single cysts in the lung and liver showed the best response. multiple, complex cysts and bone cysts showed little or no objective improvement. one patient developed reversib ...19846696172
[hydatid cysts of the liver].single or multiple hydatid cysts of the liver are frequently observed in italy and the mediterranean countries. they are swellings caused by the hexacanth larva of a small cestode, echinococcus granulosus. this must be distinguished from e. multilocularis, which is responsible for alveolar cyst, and is common in central-eastern europe, but rare in italy. an account is given of the development cycle of the intermediate and final host, the protean symptomatology (dependent on the siting of the cys ...19846709221
[chemotherapy of human echinococcosis].in human echinococcosis, today as in the past, surgical removal of the parasite remains the treatment of choice. beside certain conditions, adjuvant chemotherapy with vermox is indicated for echinococcus multilocularis as well as echinococcus granulosus. a substantial decrease of the echinococcal parasite mass under mebendazole is not yet known. nevertheless jaundiced patients with diffuse liver infestation with echinococcus multilocularis became jaundice-free and able to work. mebendazole toler ...19846714007
issues in clinical parasitology: the management of hydatid cyst.echinococcal or hydatid cyst is not commonly seen in the united states; however, two cases were seen within a 6-month period at a southern teaching hospital, and it is endemic in many areas of the world. the causative organism, echinococcus granulosus, is a tapeworm of dogs; the normal intermediate hosts are hoofed animals. when man becomes infected enlarging cysts in the liver, lung, nervous system, or peritoneum may cause considerable morbidity. while surgery traditionally has been the treatme ...19846720661
[flubendazole in human echinococcus granulosus hydatidosis. preoperative care: parasit-pharmacologic study].the effect of flubendazole in human hydatic disease due to e. granulosus has been studied in 22 patients with one or several hydatic cysts of different sites. flubendazole has been given orally before surgery for a 10 days mean period, at 4 g daily in adults and 1 g daily in children. flubendazole has been titrated by radioimmuno-assay in sera, urines and bile taken 12 to 24 hours after the last ingestion and in the hydatic cyst (membranes and liquid). the scolex vitality was determined by direc ...19846722962
sequential morphologic changes in adult echinococcus granulosus during complement-mediated lysis in vitro. 19846735577
absence of echinococcus granulosus in dogs from central sulawesi (celebes) indonesia. 19846737166
membrane permeability of secondary hydatid cysts of echinococcus granulosus. determination of the water diffusional and osmotic permeability coefficients through a syncytial membrane.diffusional (pw) and osmotic (pf) water permeability coefficients were determined for the syncytial epithelium of larval echinococcus granulosus. pw was calculated from simultaneous influx measurements of tritiated water and n-[14c]butanol through the hydatid cyst wall. the total diffusional water permeability coefficient, p'w, was found to be 2.2 x 10(-4) cm s-1; which is similar to that previously reported by rotunno et al. (1974, j. parasitol. 60, 13-620). nevertheless, when p'f is corrected ...19846749186
isolation of fraction no. 10 from taenia saginata and evaluation of its specificity for the diagnosis of bovine cysticercosis.in an attempt to prove the specificity of the crude taenia saginata antigen for the immunodiagnosis of bovine cysticercosis, a major and highly immunogenic fraction (f10), responsible for the formation of the typical "long band" reaction in immunoelectrophoresis, has been isolated from t. saginata proglottides by immunoaffinity chromatography. the immunoabsorbent was prepared by coupling a specifically raised hyperimmune serum (his) anti-f10 to sepharose 4b. the purity of the isolated f10 was de ...19816798773
isolation of two lipoprotein antigens from the metacestodes of taenia hydatigena (pallas, 1766) and taenia multiceps (leske, 1780) and their evaluation in sero-diagnosis.two identical host contaminant-free lipoprotein antigens were isolated from the metacestodes of t. hydatigena and t. multiceps, of which antigen 1 was more reactive than antigen 2. these antigens appear to be identical with antigens b and a, respectively, and antigen 2 with arc 5 of echinococcus granulosus described by other workers. attempts to use these antigens in serodiagnosis showed that only in the indirect haemagglutination (iha) technique was there any increased sensitivity and specifici ...19826819681
hydatid disease in the western isles.an investigation into the incidence of hydatid disease was carried out in the hebridean islands of lewis and skye. the results showed that 20% of the sheep were infected and 10% of the dogs harboured echinococcus granulosus. sporadic human cases continue to occur but using serological tests we were unable to show evidence of subclinical infection in the population sampled.19836822726
resection versus pericystectomy in the treatment of hydatidosis of the liver.the surgical treatment of 87 hepatic cysts from echinococcus granulosus in 42 patients has been presented. the importance of determining the features of the cysts in each patient (age, number, location, and relation with vasculobiliary structures) in order to carry out the most appropriate treatment was stressed. radical surgery was preferred because marsupialization, a conservative technique still widely used, has a high complication rate (60 percent in this study) and a prolonged recovery time ...19836824138
an active intermediate host role for man in the life cycle of echinococcus granulosus in turkana, kenya.hydatid material removed at operation from 97 turkana was compared for fertility and viability with hydatid material removed from camels, cattle, sheep, and goats. the results showed that the human material was extremely fertile and viable, as was material from the camels, goats, and sheep. cattle cysts were invariably sterile, and the protoscoleces, when present, were comparatively less viable. the high incidence and fertility of hydatid cysts in the turkana, together with the lack of burial cu ...19836837848
[complications and late results following surgical treatment of echinococcus granulosus].the case histories of 110 patients with hydatid disease were evaluated for localisation of the cysts, operative treatment and postoperative complications. pericystectomy and hydatectomy were the most frequently performed operative procedures. 1 of 37 patients, who were followed up, showed a relapse (2.7%). if consideration is taken of the postoperative complications, hydatectomy is a suitable operative procedure for hydatid disease in comparison with pericystectomy.19836851754
comparative strobilar development of echinococcus granulosus of sheep origin from different geographical areas of australia in vivo and in vitro. 19836853016
echinococcus granulosus: hook-muscle systems and cellular organisation of infective oncospheres. 19836874228
echinococcus granulosus equinus: an ultrastructural study of the laminated layer, including changes on incubating cysts in various media.the microfibrillate component of the laminated layer of echinococcus granulosus equinus contains, except for the zone adjacent to the germinal layer, aggregates of electron-dense bodies displaying a sub-structure of electron-lucent spheres. the tegumentary syncytial cytoplasm contains randomly distributed electron-dense granules, many occurring near the apical plasmalemma, although exocytosis was rarely seen. granules, similar in size and sub-structure to the bodies of the aggregates, also occur ...19836877866
echinococcus granulosus equinus: an ultrastructural study of murine tissue response to hydatid cysts.peritoneal hydatids of echinococcus granulosus equinus of 9 months standing in balb/c mice occurred as free cysts or cysts within cyst masses. both showed wide variation in size and in host tissue response, and all had a well-developed laminated layer separating the host tissue response from the germinal layer. in the smallest cyst-mass cysts the host tissue response was present as remnants of the initial cellular attack involving eosinophils. slightly larger cyst-mass cysts possessed a primary ...19836877867
hydatid disease in the turkana district of kenya. iii. the significance of wild animals in the transmission of echinococcus granulosus, with particular reference to turkana and masailand in kenya.the results are given of a study on the role of wildlife in the transmission of echinococcus granulosus in the turkana and narok districts of kenya. a total of 76 wild carnivores belonging to three separate species was examined from turkana district. echinococcus adults were found in 11 of 38 silver-backed jackals (canis mesomelas) and in six of 22 golden jackals (canis aureus). this is the first record of golden jackals being infected with this parasite in kenya. none of 16 spotted hyaenas (cro ...19836882057
the susceptibility of the european red fox (vulpes vulpes) to infection with echinococcus granulosus of australian sheep origin.a detailed comparative study was made of the development of echinococcus granulosus of australia sheep origin in european red foxes (vulpes vulpes) and dogs. no significant differences in growth, segmentation or maturation of the parasite between dogs or foxes was found, although worm burdens were slightly lower in foxes than in dogs. it is concluded that although the role of foxes in the epidemiology of hydatidosis in australia may not be significant, foxes should nevertheless be regarded as po ...19836882058
a study of immunoregulation of balb/c mice by echinococcus granulosus equinus during prolonged infection.two models of intraperitoneal infection with e. granulosus equinus by protoscolices and by cyst passage in balb/c mice were used to provide mesenteric lymph node cells for adoptive cell transfer into syngeneic recipient normal responder mice. the cell transfer inocula were shown to have depleted thy-1 cells, but to be highly suppressive to the normal sheep erythrocyte response of the recipients. the nature of the depletion and non-specific suppression, and the infectious nature of the latter, ar ...19816972509
[a danish pathography from the 17th century (johan valentin wille) (author's transl)].a monograph written by the physician johan valentin wille in 1675 is discussed and commented. the monograph was published in th. bartholin acta medica, the first medical journal in denmark, and was in 1934 translated from latin into danish by the physician eiler høeg. it givees a detailed description of organ changes and internal parasites found in cattle and hare autopsied on sealand 1674-75. vermiform bodies found in the liver of cattle are undoubtedly identical with fasciola hepatica. the tum ...19806987614
field trials with nitroscanate against cestodes and nematodes in dogs.clinical trials were carried out to test the safety and efficacy of the anthelmintic, nitroscanate, against naturally acquired helminth infections. the trials involved 418 dogs, of which 252 were treated with one or other of two different tablet formulations of nitroscanate. a coarse particle formulation given in two doses each of 200 mg per kg reduced the worm burden of taenia spp and dipylidium caninum by 100 per cent and echinococcus granulosus by 94.1 per cent in farm dogs. given as a single ...19806990596
terelisa: the elisa test performed in terasaki plates.the use of terasaki (10 microliter samples) and microtitration (100 microliter samples) plates as the solid phase in enzyme immunoassays was compared. various antigens were used for coating the plates and antibodies present in human sera were evaluated using the same anti-human ig antibody labelled with either beta -galactosidase, alkaline phosphatase, peroxidase or glucose oxidase. the results obtained, either by scoring with the naked eye or by absorbance reading with appropriate densitometers ...19817031134
chemotherapy for larval echinococcosis in animals and humans: report of a workshop.mebendazole, its fluorine analogue flubendazole, and other benzimidazole derivatives are active against many gastrointestinal and tissue-stage helminths. this article reviews the published literature and proceedings of a workshop on the use of benzimidazoles against larval echinococcosis (hydatid disease). orally administered high doses (30-50 mg/kg body weight) of mebendazole given daily for 20-90 days to rodents or sheep infected with larval echinococcus granulosus cause damage of destruction ...19827041454
intraspecific variation in echinococcus granulosus: the australian situation and perspectives for the future.the current status of knowledge of speciation and strain differentiation in echinococcus is discussed, with particular reference to the australian scene. a new approach to the problem using morphological, developmental and biochemical differences is described and the importance of correct identification for the practical aspects of controlling the disease is stressed.19827043803
simulating strategies for control of echinococcus granulosus, taenia hydatigena and t. ovis.a deterministic model to compare various control strategies for parasites having two hosts is presented. when applied to echinococcus granulosus, taenia hydatigena and t. ovis, the model shows that maximum progress would be achieved when both the definitive and the intermediate host are treated simultaneously. this type of model may prove of value in studies on the control of other two-host parasites.19807052222
immune recognition of echinococcus granulosus. 1. parasite-activated, primary transformation by normal murine lymph node cells.culture of murine lymph node cells together with living protoscolices of echinococcus granulosus is described. the presence of the parasite induced potent blastic transformation in lymphocytes of unimmunized mice as indicated by tritiated thymidine incorporation. the response was markedly reduced by killing the parasite immediately prior to culture. no blastogenic activity was detectable in supernatants from living parasites cultured alone. protoscolices from artificially infected syngeneic mice ...19827058065
intermediary carbohydrate metabolism in protoscoleces of echinococcus granulosus (horse and sheep strains) and e. multilocularis.with few exceptions, the specific activities of the glycolytic enzymes and the steady-state content of glycolytic and associated intermediates in protoscoleces of the horse (e.g.h) and sheep (e.g.s) strains of echinococcus granulosus and the closely related e. multilocularis (e.m.) are very similar. phosphorylase, hexokinase, phosphofructokinase and pyruvate kinase catalyse non-equilibrium reactions and the patterns of activity for pyruvate kinase, phosphoenolypyruvate carboxykinase and malic en ...19827070845
echinococcus granulosus: the influence of mebendazole therapy on the ultrastructural morphology of the germinal layer of hydatid cysts in humans and mice.upon ultrastructural examination it was found that prolonged mebendazole therapy of patients with hydatid disease often resulted in complete necrosis of the germinal layer of echinococcus granulosus cysts. the affected cysts showed only remnants of degenerated germinal layer tissue including heterogeneous vesicular membranes, electron-dense amorphous structures, myeloid bodies, lipid globules, crystal-like precipitates and remnants derived from muscular tissue. examination of the hydatid cysts o ...19827072321
[echinococcus granulosus infecting lung and liver. report of a case (author's transl)]. 19827087270
the prevalence of dirofilaria immitis and other parasites in urban pound dogs in north-eastern victoria.the results of a survey investigating the prevalence of dirofilaria immitis and other helminth and protozoan infections in urban dogs in north-eastern victoria are presented. d. immitis was detected in 2.7% of the general population (8.3% of dogs older than 2 years). microfilariae of d. immitis were not detected in the peripheral circulation of 30% of dogs infected with the adult parasites. the most prevalent gastro-intestinal parasites were dipylidium caninum 57%; trichuris vulpis 41%; toxocara ...19827115227
the application of a food incorporating praziquantel in the treatment and control of echinococcus granulosus infections in dogs. 19827115229
the use of a cathodic antigen in the immunoelectrophoretic serodiagnosis of echinococcus granulosus in sheep.an antigen was prepared from purified sheep hydatid-cyst fluid by gel-filtration on sephadex g-200 followed by chromatography on deae-cellulose. in each case the first-peak material was used. this antigen, which migrated cathodically, was concentrated and used in immunoelectrophoretic analyses of 4x concentrated sera from sheep experimentally and naturally infected with echinococcus granulosus and from uninfected sheep. of 34 sheep with e. granulosus infection 31 were positive with the cathodic ...19827123842
possible factors influencing distribution and prevalence of echinococcus granulosus in utah.a descriptive epidemiologic study was designed to test an impression that infections of echinococcus granulosus in dogs, sheep, and human beings were concentrated in central utah, and to determine when and how the parasite was introduced into the state, which factors were involved in the distribution of the cestode, and which factors increased either a person's risk of infection of the number of people at risk. data were secured by review of available records, interviews, questionnaires, and fie ...19827124714
characterization of glutathione s-transferase activity in echinococcus granulosus.1. glutathione s-transferase activity has been characterized in protoscolices of echinococcus granulosus. 2. the conjugation reaction was activated by treatment of protoscolices with phenobarbital and beta-naphthoflavone. 3. protoscolices of e. granulosus contained the conjugation cofactor glutathione in the amount of 287 nmol/g fresh tissue. 4. conjugation may represent an important factor in the resistance of the parasite to drugs.19827128103
immunisation of lambs against echinococcus granulosus using antigens obtained by incubation of oncospheres in vitro. 19827134642
[echinococcus granulosus -- initial manifestation as anaphylactic shock]. 19827144499
a ten-year survey of hydatid disease (echinococcus granulosus) in children.during the period 1966-1976, 32 children from 6-16 years of age were admitted to the surgical service of nemazee hospital for hydatid disease. there were 16 males and 16 females. eighteen patients had cysts of the liver, 13 had cysts of the lungs and 1 had a cyst of the orbit. sixteen patients each had multiple cysts and 16 each had a single cyst. the location of multiple cysts were: liver in 10 patients, liver and kidney in 1, liver and cul-de-sac in 1, lungs in 3, lung and spine in 1 patient. ...19827146453
[epidemiology of echinococcosis in domestic animals and man in bulgaria].echinococcosis is an important agricultural and public health problem in the people's republic of bulgaria. organized and purposeful control since 1960 has proved to be effective. in the investigated period from 1975 to 1979 a decreased infestation with echinococcus granulosus of man and farm animals could be observed. infestation degree of sheep is below 20% in flat areas, whereas in mountainous regions it is well over 50%. the infestation of the population with echinococcosis is not equally di ...19827149329
a study of the microtriches of adult echinococcus granulosus by scanning electron microscopy. 19827160960
the effects of sustained release praziquantel on the survival of protoscolices of echinococcus granulosus equinus in laboratory mice. 19827171252
infectious agents in spinal epidural abscesses.of 27 cases of spinal epidural abscess, 19 were caused by bacteria, 7 by mycobacterium tuberculosis, and 1 by echinococcus granulosus. blunt trauma and cutaneous infections were the most frequent preceding events in bacterial cases. tuberculous abscess was usually the sole manifestation of reactivation of dormant tuberculosis. drug addiction, the most common cause in young adults, was associated with gram-negative infections. whatever the infectious agent, paraparesis for longer than 4 days led ...19807191067
maintenance of the life cycle of echinococcus granulosus in the laboratory following in vivo and in vitro development. 19817195632
orbital hydatid cyst.a six-year-old bantu child presented with a mass in the right orbital region, proptosis, and moderate pallor of the optic disk. orbital exploration revealed an unsuspected hydatid cyst. the characteristic histopathologic features of the organism echinococcus granulosus are emphasized.19807205519
effect of mebendazole against echinococcus granulosus and taenia hydatigena cysts in naturally infected sheep and relevance to larval tapeworm infections in man.the ability of three treatment schedules of mebendazole to kill well-established hydatid cysts was studied. pregnant sheep, naturally infected with echinococcus granulosus and/or taenia hydatigena, were treated daily with mebendazole at a dose rate of 50 mg/kg body weight for either five days, one month, or three months. at autopsy, seven months after the commencement of treatment, no evidence was found that the 5-day treatment schedule had any damaging effect on e. granulosus cysts. the effects ...19817210815
echinococcus granulosus: diagnosis of human hydatid disease by the indirect haemoagglutination reaction with antigens from hydatid fluid and scoleces. 19817228479
taeniasis, cysticercosis and echinococcosis/hydatidosis in nigeria: iv--prevalence of echinococcus granulosus infection in stray dogs.echinococcus granulosus (batsch, 1786; rudolphi, 1801) infections in stray dogs were recorded in the sudan and northern guinea zones at a prevalence rate of 6.2% and 1.2% respectively. the parasite was not recorded in any stray dog obtained from the bauchi plateau zone, although fewer dogs were examined from this zone. the high prevalence of echinococcosis in dogs reported in the sudan zone bears a true epidemiological relationship with the high prevalence of cystic hydatidosis recorded for slau ...19807229324
[the large island--impressions of a medical parasitological trip to madagascar].the course of an investigation trip through madagascar is sketched. it allowed to analyze the parasitological situation. it is explained with the sociological and economical facts and the unusual fauna of this island. the two biggest problems are malaria tropica and schistosomatosis (bilharziasis). further, ancylostoma, ascaris, taeniids, and wuchereria occur. plague is rare. fasciola gigantica has been introduced since short time only. introduction of other parasites must be feared. sleeping il ...19817235291
cytotoxicity of echinococcus granulosus cyst fluid in vitro.in a cytotoxicity assay, using rat spleen cells as target cells, echinococcus granulosus cyst fluid from cattle exerted a marked degree of cytotoxicity in vitro. when trypan blue exclusion or [3h]thymidine incorporation by concanavalin a stimulated spleen cells was used as a measure of cell viability, dialyzed cyst fluid showed maximum cell destruction up to a 1:8 dilution. the effect was dose and time dependent, cells being affected by 24 h after exposure to cyst fluid. the components responsib ...19817245844
hydatid disease in ethiopia: clinical survey with some immunodiagnostic test results.after surveying various regions of ethiopia using clinical examinations, filter paper indirect hemagglutination (iha) test, and hydatid skin test, a hyperendemic focus of hydatid disease (echinococcus granulosus) was found in southwestern ethiopia. two tribes, the dassanetch and nyangatom, in the lower omo river valley were found to have a particularly high prevalence of the disease. of 1,342 individuals from these two tribes 4.8% had palpable abdominal cysts and 15% had hepatomegaly. this contr ...19817258484
echinococcus granulosus: permeability of hydatid cysts to mebendazole in mice. 19817275474
vertebral hydatid disease: radiological assessment.the computed tomographic (ct) appearance and the pain radiographic and myelographic findings of vertebral hydatid disease (caused by echinococcus granulosus) in two patients are presented. ct proved to be more useful in the initial assessment and measurement of progress of this disease than conventional radiography and myelography.19817280247
experimental infection on sheep and pigs with echinococcus granulosus (batsch, 1786), and the origin of pouching in hydatid cysts. 19817282497
echinococcus granulosus cysts: early development in vitro in the presence of serum from infected sheep. 19817287291
[pharmacokinetic study of flubendazole in human hydatid disease caused by echinococcus granulosus. preliminary results (author's transl)].a pharmacokinetic study of flubendazole was carried out, using a radioimmunological method, in patients with hydatid disease about to undergo surgery; there was no evidence of drug penetration into the walls or contents of the cysts. another study, carried out simultaneously in subjects without hydatid disease, showed very high concentrations of the drug in liver tissue and in bile. these results should not be taken as arguments against the effectiveness of flubendazole, but should encourage the ...19817290973
hepatobronchial fistula caused by hydatid disease. the dunedin experience 1952-79.despite intensive hydatid control measures in new zealand, hepatopulmonary fistula resulting from infection by the echinococcus granulosus still occurs. although the patients may quickly become debilitated from coughing bile and pus and associated septic complications, appropriate surgical therapy is usually effective. the exact diagnosis may be obscure, but it is helped by a high index of suspicion. a retrospective study of eight patients seen over a 27-year period is presented, and principles ...19817292377
water and electrolyte balance in protoscoleces of echinococcus granulosus incubated in vitro: general procedures for the determination of water, sodium, potassium and chloride in protoscoleces. 19817309341
water and electrolyte balance in protoscoleces of echinococcus granulosus incubated in vitro: effect of metabolic inhibitors. 19817309342
treatment of hydatid cysts (echinococcus granulosus) by cetrimide (r).cetrimide (r) has been efficiently assessed against hydatid protoscolices as a scolicidal adjunct to hydatid surgery. it was tested in four concentrations (0.05, 0.1, 0.5 and 1%) against the protoscolices of hydatid cysts in sheep and cattle and in 0.1% concentration against the protoscolices of hydatid cysts in humans. for each concentration, three incubations of one, five and 10 min exposure were tested in vitro and in vivo on sheep and cattle cysts, and two successive incubations each of five ...19817324114
effect of praziquantel on adult echinococcus granulosus in vitro: scanning electron microscopy.the effect of praziquantel in vitro at concentrations of 5, 50 and 500 ppm for 1 h resulted in the progressive breakdown of the tegument and in morphologic distortion of adult echinococcus granulosus when compared to controls. scanning electron microscopy of all specimens treated in the various concentrations of praziquantel showed loss of most, if not all of the rostellar hooks and changes in the structure of the suckers. many of the tapeworms immediately detached from the host's gut upon being ...19817324547
resistance to echinococcus granulosus infection in lambs.a high level of resistance of oral infection with echinococcus granulosus eggs was stimulated in lambs by two or more subcutaneous injections of oncospheres given 14 days apart. the degree of resistance was significantly higher than that resulting from a single injection. resistance was apparently stimulated by the activated oncosphere or a stage of cyst development prior to 14 days of age. studies on oncospheres cultured in vitro in sera collected from animals during immunization or after oral ...19817328452
murine hybridoma-derived antibodies in the processing of antigens for the immunodiagnosis of hydatid (echinococcus granulosus) infection in sheep.mice experimentally infected with hydatid parasite echinococcus granulosus (secondary echinococcosis), were used as spleen cell donors for hybridoma production. two hybridomas were produced which secreted antibody with anti-e. granulosus cyst fluid (egcf) activity. radioimmunoassays comparing the binding of these hybridoma-derived antibodies to e. granulosus versus several other antigen preparations from sheep parasites showed that the antibodies had a high degree of specificity for the hydatid ...19817329721
experimental transmission of echinococcus granulosus of "camel-dog" origin to goats, sheep, cattle and donkeys.the infectivity of a camel strain of echinococcus granulosus experimentally raised in dogs for domesticated food and burden animals was investigated. of the animal species that were inoculated orally with gravid segments, hydatid cysts developed poorly in goats and sheep, less well in cattle and not at all in donkeys by six month post-inoculation. cysts were most frequently found in the lungs, although other organs such as liver and spleen also contained cysts. histological examination of affect ...19817333783
characterization of experimentally raised echinococcus granulosus of "camel-dog" origin. 19817333784
[encapsulation and shape of young hydatids of echinococcus granulosus in a spontaneous infection of pigs]. 19817347963
a morphological study on hydatids of echinococcus granulosus (batsch, 1786) from pigs. 19807364344
echinococcus granulosus in dogs in bangladesh.a survey was carried out during the period february 1975 to january 1978 to find the frequency of echinococcus granulosus in dogs. a total of 611 dogs originating from different places of the country were examined. the frequency of infection varied considerably, the highest (75.79%) being recorded in dogs in and around the slaughterhouses. the average frequency was 62.52%. the seasonal variations of the infection were determined, the lowest (47.09%) and the highest (78.37%) being january to marc ...19807369616
[evaluation of helminth intradermal tests in comparison to coproovoscopic surveys].1600 subjects connected with mining were divided into 6 groups and examined because of intestinal parasites. there were: surface miners underground miners, retired miners and two groups of school-children up to 14 years old and from 14-17 years old, and persons not connected with the miners' work. the one control group consisted of 150 persons with negative coproscopical investigations, the other consisted of 112 persons, who were clinically healthy. the coproscopical investigations, morphologic ...19807377624
[differential diagnosis of cysticerci of taenia hydatigena and hydatids of echinococcus granulosus].in combined infestations of pig by hydatids as well as larvae of taenia hydatigena macroscopic and histological differences were stated which are of great importance for differential diagnosis of these two infestations in veterinary practice.19807377625
experimental infection of mice with echinococcus granulosus equinus of british origin. 19807377878
ultrasonography of echinococcus granulosus cyst of the liver. 19807381292
serologic cross-reactions with sera from patients with echinococcosis and cysticercosis.serum specimens from persons with pathologically confirmed echinococcus granulosus hydatidosis and taenia solium cysticercosis were tested in indirect hemagglutination tests using both echinococcus and taenia antigens. there were cross-reactions with most sera, and it was not possible to distinguish specific reactions by comparing titers with the homologous or heterologous antigens. using immunoelectrophoresis or double diffusion tests with e. granulosus antigens, the echinococcus-specific arc 5 ...19807406111
echinococcus granulosus infection and malignancy. 19807407519
the lipids and phospholipids of hydatid protoscolices of echinococcus granulosus (cestoda). 19807409977
treatment of hydatid disease with mebendazole at frere hospital, east london. a preliminary report.the high incidence in south africa of hydatid disease caused by echinococcus granulosus is indicated. preliminary experience in the treatment of cysts with high doses of mebendazole (vermox; ethnor) is presented. the low toxicity of mebendazole as reported in the literature is confirmed.19807423294
hybridoma antibody immunoassays for the detection of parasitic infection: attempts to produce an immunodiagnostic reagent for a larval taeniid cestode infection.attempts have been made to produce discriminatory immunodiagnostic reagents for infections of sheep with the larval stages of taenia hydatigena, taenia ovis and echinococcus granulosus. using cells from mice hyperimmunized with t. hydatigena antigenic preparations for fusion, a hybridoma antibody which bound to t. hydatigena larval antigens, but not to t. ovis or e. granulosus larval antigens, was selected. a competitive radioimmunoassay using the labelled hybridoma antibody and a crude parasite ...19807436880
tissue reaction to spherical and lobular hydatid cysts of echinococcus granulosus (batsch, 1786).we have studied the tissue reaction to spherical and lobular cysts of echinococcus granulosus (batsch, 1786) on a set of 108 observations of hydatidosis in the liver and lung of pigs. typical of an early response of the host to a growing hydatid cyst is a local allergic inflammation characterized by a heavy infiltration of eosinophiles. if the infection is massive, sensitization increases the tissue reaction, and there is a marked exudation of eosinophiles into the pseudocyst which results in th ...19807450606
the effect of route of administration on the efficacy of praziquantel against echinococcus granulosus infections in dogs.dogs infected with echinococcus granulosus were treated with praziquantel by three routes and at different dosage rates. the results indicated that the dog was of similar efficacy when administered by the oral and intramuscular routes, but less effective by the subcutaneous route.19807455348
hydatid cyst of the pancreatic tail. ultrasonic features including application of the escudero-nemenow sign.pancreatic involvement by echinococcus granulosus is rare. a case of hydatid cyst of the tail of the pancreas is presented with the radiological and ultrasonic appearances. real-time ultrasound examination revealed an alteration in shape of the cyst with respiration, analogous to the escudero-nemenow sign in pulmonary hydatid cyst fluoroscopy.19817455858
[human echinococcosis in switzerland, 1984-1992].in a retrospective study (1984-1992), new cases of human echinococcosis were registered in switzerland based on information obtained from (a) questionnaires sent to 294 of the 300 acute hospitals in all parts of the country and to 17 institutes of pathology (268 answers form hospitals: 91%), and (b) from tracing back cases reported under the official notification system since 1 january 1988 by laboratories to the federal health office or recorded at the institute of parasitology in zurich. cases ...19957481657
further characterization of the 38 kda antigen from echinococcus granulosus (hydatid disease) cyst fluid: evidence for antigenic heterogeneity and reactivity with anti-p1 antibodies.a panel of 5 igm and 6 igg1 monoclonal antibodies (moabs) was produced against a band, eluted from a reducing sds-page gel, containing the 38 kda subunit of antigen 5 (ag 5) from echinococcus granulosus cyst fluid; seven of the moabs were shown subsequently to bind epitopes on ag 5 but none recognized phosphorylcholine or periodate-sensitive carbohydrate epitopes. differences in the fine specificity of the moabs were apparent and, upon reduction, heterogeneity in 38 kda components from hydatid c ...19957494641
cloning and immunological characterisation of echinococcus granulosus ferritin.a cdna clone of echinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. the deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin. e. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 da and a statistical isoelectric point of 7.6. functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. i ...19957501636
anti-tubulin immunohistochemistry study of echinococcus granulosus protoscolices incubated with albendazole and albendazole sulphoxide in vitro.tubulin content was immunohistochemically studied in echinococcus granulosus protoscolices incubated with albendazole and albendazole sulphoxide alone or in combination. tubulin immunoreactivity was very patent in the control protoscolices and was mainly located in the sucker region and the invagination channel of protoscolices. treated protoscolices always showed less immunoreactivity than did control protoscolices. we concluded that albendazole and its metabolite albendazole sulphoxide produce ...19957501645
[acute pancreatitis due to the rupture of an echinococcal cyst into the bile duct system].a 47-year-old woman with stones in the gall-bladder suddenly developed severe upper abdominal pain. cholesterol concentration was elevated, as were amylase (555 u/l) and lipase (408 u/l) concentrations, suggesting biliary pancreatitis. endoscopic retrograde cholangiography demonstrated a cyst, about 10 cm in diameter, in the left lobe of the liver, connected to the biliary tract system. ultrasonography and computed tomography additionally showed a smaller cyst in the right lobe. infection with e ...19947513277
identification and diagnostic value of a major antibody epitope on the 12 kda antigen from echinococcus granulosus (hydatid disease) cyst fluid.an igg1 monoclonal antibody (moab), designated c9e7h8, has been produced against an epitope on the 12 kda antigen of echinococcus granulosus cyst fluid, believed to represent the smallest subunit of antigen b. this moab, raised against purified 12 kda antigen eluted from a reducing sds-page gel, demonstrated strong binding to native sheep cyst fluid in elisa and recognition of all three subunits of antigen b (at 12, 16, 23 kda) by immunoblot under both reducing and non-reducing conditions. immun ...19947517028
cellular and humoral responses to antigenic subunits of echinococcus granulosus cyst fluid in hydatid patients.the hydatid fluid antigenic subunits which stimulate the proliferative response of t cells in pbmc isolated by hydatid patients were identified. in the same subjects, the serum igg antibody profiles were determined by immunoblotting to compare t cell activation and igg production. the study was carried out on 18 patients with clinically and serologically diagnosed hydatidosis and on ten healthy blood donors. to assess the proliferative response to the different antigenic subunits, sheep hydatid ...19947528907
chromatographic and antigenic properties of echinococcus granulosus hydatid cyst-derived glycolipids.the neutral and acidic fraction glycolipids of echinococcus granulosus metacestode tissue compartments were isolated, defined by their chromatographic and antigenic properties, and assessed as to their efficacy as antigens in the serodiagnosis of human hepatic cystic and alveolar echinococcosis, and other helminthiases. analyses were accomplished by thin-layer chromatography immunostaining and elisa. the neutral glycolipid fraction's major carbohydrate epitope was the same as or very similar to ...19937533281
serotoninergic and peptidergic nerve elements in the protoscolex of echinococcus granulosus (cestoda, cyclophyllidea).the localisation and distribution of 5-hydroxytryptamine (5-ht, or serotonin) and neuropeptides in the nervous system of the protoscolex of the hydatid organism echinococcus granulosus were determined by an indirect immunofluorescence technique. nerve-cell bodies immunoreactive for 5-ht occurred in the lateral ganglia and in association with the lateral longitudinal nerve cords. 5-ht immunostaining was also evident in the central nerve ring, in the rostellar nerves and in the nerve plexus innerv ...19947533908
epidemiological studies on cystic echinococcosis in china--a review.in the four decades from 1951 to 1990, the six provinces or autonomous regions (xinjiang, gansu, qinghai, ningxia, xizang and nei monggol) reported a total of 26,065 surgical cases of hydatid disease, most of which were reported in the recent decade. about one third of the patients was children and adolescents under 15 years old. so far, cystic hydatid infections of local origin have been confirmed in 22 provinces, autonomous regions and municipalities in the whole nation. findings of x-ray exam ...19957546341
an imported case of hepatic unilocular hydatid disease.a 38-year old man visited a private clinic complaining of epigastric discomfort for 2 months. a huge hepatic cyst was found by sonography and computerized tomography. an exploratory laparotomy was performed under the impression of hydatid disease. the cyst was successfully removed. a lot of living protoscolices of echinococcus granulosus were found from the cystic fluid under light microscopy. during the operation, however, the cyst was accidentally ruptured and the cystic fluid spilled out. the ...19957551803
effects of mebendazole, albendazole and praziquantel on alanine aminotransferase and aspartate aminotransferase of echinococcus granulosus cyst wall harbored in mice.alanine aminotransferase (alt) and aspartate aminotransferase (ast) of echinococcus granulosus cyst wall harbored in mice for 10-12 months was measured. the activities of the 2 enzymes were 3,430 +/- 1,370-4,160 +/- 1,790 and 1,560 +/- 840-2,890 +/- 1,470, respectively. when infected mice were treated ig with mebendazole (meb) 25 mg.kg-1.d-1 for 7-14 d, or 50 mg.kg-1.d-1 for 7 d, the alt activities of cyst walls of both collapsed and full cysts were inhibited significantly with average inhibitio ...19957554156
transmission dynamics of echinococcus granulosus, taenia hydatigena and taenia ovis in sheep in uruguay.a base-line survey was carried out on the transmission dynamics of echinococcus granulosus, taenia hydatigena and taenia ovis in sheep in the department of florida, uruguay. mean life expectancy at birth of the sheep population in this department was estimated at 3.5 and 4.8 years for male and female sheep, respectively. both intensity and prevalence of e. granulosus infection increased with age providing no evidence that immunity was acquired in the population. this revealed that e. granulosus ...19957558566
[endoparasite infection in stray and abandoned dogs in southern switzerland].at their entry into the animal domicile, "la stampa", in lugano (canton tessin), 217 stray dogs and 154 unwanted dogs were examined for infections with intestinal parasites, filariae, babesia and leishmania. the following techniques were used for detection of intestinal parasites: combined sedimentation-flotation, mifc technique and scotch tape adherence test. prevalences of helminth egg excretion in stray dogs and in unwanted dogs, respectively, were as follows: 34% and 22% for trichuris, 17% a ...19957569839
epidemiological studies on intestinal helminth parasites of rural and urban red foxes (vulpes vulpes) in the united kingdom.an epidemiological study of intestinal helminths in 843 foxes (vulpes vulpes) from southern england revealed the presence of 13 parasite species: five nematodes--toxocara canis (prevalence 55.9%), toxascaris leonina (1.5%), uncinaria stenocephala (68.0%), trichuris vulpis (0.5%) and capillaria aerophila (0.2%); four cestodes--taenia pisiformis (13.8%), taenia hydatigena (2.5%), echinococcus granulosus (0.1%) and dipylidium caninum (3.8%); two trematodes--brachylaima recurva (2.9%) and cryptocoty ...19957571336
[praziquantel in the treatment of human echinococcosis].in the period from 1989 to 1993, 73 patients with echinococcus granulosus cysts were treated by prazyquantel. to differentiate cysts tests of indirect immunofluoroscence and hemagglutination were used. the tests were performed during and after the completed treatment. during the treatment three patients were excluded so that 70 patients completed the treatment. fifty five (78.9%) patients were treated only with drug with a success of 67% (37 patients). the preventive treatment, due to cyst perfo ...19957571529
[a retroperitoneal hydatid cyst--a rare manifestation of echinococcus granulosus].a rare case of a retroperitoneal cyst of echinococcus granulosus in a male adult is presented. due to its extension and infiltration of important anatomical structures the hydatid cyst could not be completely removed by operation. therefore the patient was treated with albendazole for 10 months after surgery. the efficiency of this combined therapy was documented by ct-scan, specific blood tests and histopathological findings.19957571900
[hepatic hydatidosis in madagascar: a case of autochthonous origin].hydatid disease, due to the larval stage of echinococcus granulosus, is described for the second time at liver level, in madagascar. even with a low level of endemic animal disease and an uncontrolled population of stray dogs, the human parasitosis is very rare in madagascar. it is interesting to note that, since a hundred of years, the epidemiological status of this zoonosis has not really changed in the great island.19947575029
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