| activity of tribendimidine and praziquantel combination therapy against the liver fluke opisthorchis viverrini in vitro and in vivo. | opisthorchiasis, caused by the liver fluke opisthorchis viverrini, a food-borne trematode, is an important public health problem; however, only a single drug, praziquantel is available. we investigated tribendimidine-praziquantel combinations against o. viverrini in vitro and in vivo. the ic50 values of 0.16 μg/ml and 0.05 μg/ml were determined for praziquantel and tribendimidine, respectively, against adult o. viverrini in vitro. when o. viverrini was exposed to both drugs simultaneously (using ... | 2013 | 22892101 |
| examining landscape determinants of opisthorchis viverrini transmission. | liver fluke (opisthorchis viverrini, o.v.) infection, along with its associated cholangiocarcinoma, is a major public health problem in southeast asia. despite the vast amount of epidemiological research, human o.v. prevalence remains high and varies greatly across the region. this paper examines the landscape determinants that influence o.v. transmission in relation to the three hosts of its life cycle and identifies areas that require further research so as to advance the understanding of the ... | 2012 | 22890564 |
| immunosuppressive prednisolone enhances early cholangiocarcinoma in syrian hamsters with liver fluke infection and administration of n-nitrosodimethylamine. | chronic infection with opisthorchis viverrini for many years has been associated with the development of hepatobiliary diseases including cholangiocarcinoma. it is well known that inflammation is a key component of the tumor microenvironment, and that chronic inflammation plays an important role in tumorigenesis. therefore, in this study cholangiocarcinogenesis was induced in syrian hamsters in order to observe the cancer-related inflammation. the syrian hamsters were divided into 5 groups: unin ... | 2013 | 22851165 |
| carcinogenic human liver fluke: current status of opisthorchis viverrini metacercariae in nakhon ratchasima, thailand. | opisthorchis viverrini infection is a serious public-health problem in southeast asia. it is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. | 2012 | 22799311 |
| exome sequencing of liver fluke-associated cholangiocarcinoma. | opisthorchis viverrini-related cholangiocarcinoma (cca), a fatal bile duct cancer, is a major public health concern in areas endemic for this parasite. we report here whole-exome sequencing of eight o. viverrini-related tumors and matched normal tissue. we identified and validated 206 somatic mutations in 187 genes using sanger sequencing and selected 15 genes for mutation prevalence screening in an additional 46 individuals with cca (cases). in addition to the known cancer-related genes tp53 (m ... | 2012 | 22561520 |
| chromosomes and karyotype analysis of a liver fluke, opisthorchis viverrini, by scanning electron microscopy. | opisthorchis viverrini, a human liver fluke, has been categorized as the carcinogenic organism according to the strong association with carcinogenesis of cholangiocarcinoma (cca). the infection of this food-borne parasite is a major impact on the health of humans, especially cca patients in the northeast of thailand. taxonomy, morphology, epidemiology and molecular study of o. viverrini have been publicized increasingly but the precise karyotypic study is still incomplete. in this study, the chr ... | 2012 | 22504416 |
| exceptionally high prevalence of infection of bithynia siamensis goniomphalos with opisthorchis viverrini cercariae in different wetlands in thailand and lao pdr. | the carcinogenic liver fluke, opisthorchis viverrini, requires bithynia snail intermediate hosts in its life cycle. however, the prevalence of o. viverrini in snail intermediate hosts is typically low (< 1%). here, we examined b. siamensis goniomphalos from 48 localities in thailand and the lao people's democratic republic (lao pdr) and reported high-prevalence levels of o. viverrini. the highest-prevalence levels per locality were 6.93% (mean = 3.04%) in thailand and 8.37% (mean = 2.01%) in lao ... | 2012 | 22403318 |
| anti-inflammatory, antioxidant and hepatoprotective effects of thunbergia laurifolia linn. on experimental opisthorchiasis. | thunbergia laurifolia linn (rang chuet) possesses antioxidant and anti-inflammatory properties as well as anticancer activities. the aim of the present study was to evaluate the efficacy of t. laurifolia in reducing inflammation from pathological changes in syrian hamsters infected with the human liver fluke opisthorchis viverrini. hamster groups were also administered n-nitrosodimethylamine (ndma) and treated with t. laurifolia. light microscopic observation of histopathological changes, liver ... | 2012 | 22327320 |
| low efficacy of single-dose albendazole and mebendazole against hookworm and effect on concomitant helminth infection in lao pdr. | albendazole and mebendazole are increasingly deployed for preventive chemotherapy targeting soil-transmitted helminth (sth) infections. we assessed the efficacy of single oral doses of albendazole (400 mg) and mebendazole (500 mg) for the treatment of hookworm infection in school-aged children in lao pdr. since opisthorchis viverrini is co-endemic in our study setting, the effect of the two drugs could also be determined against this liver fluke. | 2012 | 22235353 |
| impaired antioxidant enzyme activity and increased dna repair enzyme expression in hamster liver tissues related to cholangiocarcinoma development. | a possible mechanism of liver fluke (opisthorchis viverrini; ov) -associated cholangiocarcinoma (cca) genesis may be imbalance in responses of antioxidant enzymes and/or dna repair enzymes which are the consequence of oxidative/nitrative stress, arising from inflammatory processes. this study aimed to investigate changes in the expression patterns of antioxidant enzymes, including superoxide dismutase 2 (sod2) and catalase (cat), as well as their activities in ov-associated hamster cca tissues. ... | 2012 | 23480773 |
| prevalence and co-infection of intestinal parasites among thai rural residents at high-risk of developing cholangiocarcinoma: a cross-sectional study in a prospective cohort study. | intestinal parasitic infections (ipis) are still important to the health of thai rural residents. ipis are the cause of many chronic diseases with, for example, opisthorchiasis resulting in progression to cholangiocarcinoma (cca). this cross-sectional study in a prospective cohort study aimed to examine the prevalence and co- infection of intestinal parasites among northeastern thai rural residents, recruited into the khon kaen cohort study (kkcs), and who were residing in areas of high-risk for ... | 2012 | 23464426 |
| development of a pcr assay for diagnosing trematode (opisthorchis and haplorchis) infections in human stools. | we developed a combined conventional polymerase chain reaction (pcr) and real-time pcr (qpcr)-based assay for detecting and discriminating between opisthorchis viverrini and haplorchis taichui parasite infections. the first pcr amplifies the mitochondrial cytochrome c oxidase subunit i (coi) genes of parasites, and differential diagnosis is achieved by performing qpcr with specific primers and sybr green i. the detection limit of the assay was found to be 2.0 × 10(2) plasmid copies in a test in ... | 2017 | 27821695 |
| genetic differences among haplorchis taichui populations in indochina revealed by mitochondrial cox1 sequences. | haplorchis taichui is an intestinal heterophyid fluke that is pathogenic to humans. it is widely distributed in asia, with a particularly high prevalence in indochina. previous work revealed that the lack of gene flow between three distinct populations of vietnamese h. taichui can be attributed to their geographic isolation with no interconnected river basins. to test the hypothesis that interconnected river basins allow gene flow between otherwise isolated populations of h. taichui, as previous ... | 2016 | 27411962 |
| opisthorchis viverrini and haplorchis taichui: development of a multiplex pcr assay for their detection and differentiation using specific primers derived from hat-rapd. | specific primers for the detection of opisthorchis viverrini and haplorchis taichui were investigated by using the hat-rapd pcr method. fourteen arbitrary primers (operon technologies) were performed for the generation of polymorphic dna profiles. the results showed that a 319 bp fragment generated from the opa-04 primer was expected to be o. viverrini-specific while a 256 bp fragment generated from the opp-11 primer was considered to be h. taichui-specific. based on each sequence data, two pair ... | 2012 | 22874527 |
| nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ... | 2017 | 28163802 |
| coinfection with helicobacter pylori and opisthorchis viverrini enhances the severity of hepatobiliary abnormalities in hamsters. | persistent infection with opisthorchis viverrini causes hepatobiliary abnormalities, predisposing infected individuals to cholangiocarcinoma (cca). in addition, helicobacter pylori is highly prevalent in most countries and is a possible risk factor for cca; however, its role in enhancing hepatobiliary abnormality is unclear. here, we investigated the effects of coinfection with h. pylori and o. viverrini on hepatobiliary abnormality. hamsters were divided into four groups: (i) normal, (ii) h. py ... | 2017 | 28138021 |
| nitrative and oxidative dna damage in infection-related carcinogenesis in relation to cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells, and result in the formation of oxidative and nitrative dna lesions, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in inflammation-mediated carcinogenesis. it has bee ... | 2016 | 28050219 |
| dna damage in inflammation-related carcinogenesis and cancer stem cells. | infection and chronic inflammation have been recognized as important factors for carcinogenesis. under inflammatory conditions, reactive oxygen species (ros) and reactive nitrogen species (rns) are generated from inflammatory and epithelial cells and result in oxidative and nitrative dna damage, such as 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) and 8-nitroguanine. the dna damage can cause mutations and has been implicated in the initiation and/or promotion of inflammation-mediated carcinogen ... | 2013 | 24382987 |
| epidemiological investigation of parasitic infection of schoolchildren from six elementary schools in sakon nakhon province, thailand. | we conducted an epidemiological study of intestinal parasitic infection in 572 schoolchildren aged 4 to 12 years old from six elementary schools in sakon nakhon province, thailand from june 2013 to august 2014. we collected fecal, blood, and urine samples to investigate parasitic infection and conducted a questionnaire survey. soil samples were examined for egg contamination. fecal examination, using the formalin-ether sedimentation method, revealed that 39% of schoolchildren were infected with ... | 2017 | 28289269 |
| serum antibody ig g and ig m titers for opisthorchis felineus correlate with eggs in faeces--a comprehensive study in chuvash republic, russia. | the cholangiocarcinoma is a. the risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of opisthorchiasis. for this reason the timely detection of opisthorchiasis is important for cholangiocarcinoma prevention. there are many studies which concern the detection of pathogenesis of opisthorchis viverrini infection but a little known about opisthorchis felineus. in this study we investigate a correlation of the eggs which are found in a faeces ... | 2016 | 26838224 |
| [role of opisthorchis felineus on induction of bile duct cancer]. | opisthorchis felineus (trematoda) is widespread in the russian federation, especially in siberia, and other countries of europe. infestation of endemic area population with o. felineus reaches 80%. on animal models of the infection of closely related opisthorchis viverrini combined with the nitrosamines' intake it has been shown that the parasite induces cholangiocarcinoma. however carcinogenic potential of o. felineus is still poorly studied. the present study is aimed to investigate the role o ... | 2015 | 26016330 |
| efficacy of moxidectin versus ivermectin against strongyloides stercoralis infections: a randomized controlled non-inferiority trial. | infections with strongyloides stercoralis are of considerable public health relevance. moxidectin, a well-established drug in veterinary medicine under consideration for regulatory submission for the treatment of onchocerciasis, might serve as alternative to the most widely used ivermectin. | 2017 | 28369530 |
| opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam. | opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ... | 2017 | 28242064 |
| preliminary genetic evidence of two different populations of opisthorchis viverrini in lao pdr. | opisthorchis viverrini is a major public health concern in southeast asia. various reports have suggested that this parasite may represent a species complex, with genetic structure in the region perhaps being dictated by geographical factors and different species of intermediate hosts. we used four microsatellite loci to analyze o. viverrini adult worms originating from six species of cyprinid fish in thailand and lao pdr. two distinct o. viverrini populations were observed. in ban phai, thailan ... | 2017 | 28238124 |
| effects of albendazole, artesunate, praziquantel and miltefosine, on opisthorchis viverrini cercariae and mature metacercariae. | to explore larvicidal effects of anthelmintic drugs on opisthorchis viverrini (o. viverrini) for alternative approach to interrupting its cycle for developing a field-based control program. | 2017 | 28237476 |
| social network analysis of food sharing among households in opisthorchiasis endemic villages of lawa lake, thailand. | consumption of raw fish is a well-documented risk factor for opisthorchis viverrini infection. sharing of food, especially raw fish recipes may influence the spread of disease through a community. using social network analysis of an ego network, we investigated food sharing among households in an opisthorchis-endemic area. network centrality properties were used to explain the differences in o. viverrini transmission and control between villages with a low and high prevalence of infection. infor ... | 2017 | 28188767 |
| efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomized parallel single-blind dose-ranging trial. | | 2017 | 28174906 |
| targeting hexokinase ii as a possible therapy for cholangiocarcinoma. | overexpression of hexokinase 2 (hkii) has been demonstrated in various cancers. a number of in vitro and in vivo studies in several cancers show the significance of hkii in many cellular processes including proliferation, metastasis and apoptosis. however, the role of hkii in opisthorchis viverrini (ov) associated cholangiocarcinoma (cca) is still unknown. in the present study, the expression and roles of hkii were determined in ov associated cca. the expression of hkii was investigated in 82 pa ... | 2017 | 28131825 |
| suspected cases of cholangiocarcinoma seen in reference hospitals in lao people's democratic republic. | cholangiocarcinoma (cca) poses a significant public health problem in southeast asia, with chronic opisthorchis viverrini infection being the main risk factor. epidemiological data on cca is not available for lao people democratic republic (lao pdr), though there is a high prevalence of o. viverrini infection in the country. a hospital record-based retrospective study was carried out in six referral hospitals (four in the capital city, one in savannakhet, one in pakse) to assess the number of su ... | 2016 | 27965165 |
| glycoproteomics analysis of plasma proteins associated with opisthorchis viverrini infection-induced cholangiocarcinoma in hamster model. | to apply lectin affinity chromatography and glycoproteomics-based lc-ms/ms to preliminarily investigate the possible potential plasma biomarkers of opisthorchis viverrini (ov)-associated cca in ov/dimethylnitrosamine (dmn)-induced cca hamster model. | 2016 | 27955744 |
| uncovering the pathogenic landscape of helminth (opisthorchis viverrini) infections: a cross-sectional study on contributions of physical and social environment and healthcare interventions. | helminth infections have proven recalcitrant to control by chemotherapy in many parts of southeast asia and indeed farther afield. this study isolates and examines the influence of different aspects of the physical and social environment, and uneven intervention effort contributing to the pathogenic landscape of human opisthorchis viverrini infections. | 2016 | 27926938 |
| seasonal and spatial environmental influence on opisthorchis viverrini intermediate hosts, abundance, and distribution: insights on transmission dynamics and sustainable control. | opisthorchis viverrini (ov) is a complex-life-cycle trematode affecting 10 million people in sea (southeast asia). human infection occurs when infected cyprinid fish are consumed raw or undercooked. ov requires three hosts and presents two free-living parasitic stages. as a consequence ov transmission and infection in intermediate and human hosts are strongly mediated by environmental factors and understanding how environmental variability influences intermediate host abundance is critical. the ... | 2016 | 27880787 |
| induction of regulatory t cells by opisthorchis viverrini. | opisthorchis viverrini causes public health problems in south-east asia. recently, tgf-β and il-10 have been reported to increase in o. viverrini-infected hamsters but the sources of these cytokines are still unknown. in this study, the cd4(+) t cells in infected hamsters were investigated. it was demonstrated that il-4(+) cd4(+) t cells were significantly increased in hamster spleens and mesenteric lymph nodes (mlns) during chronic infection. interestingly, il-10(+) cd4(+) t cells were also dis ... | 2016 | 27552546 |
| health informatics model for helminthiasis in thailand. | at the beginning of the new millennium, helminth infections continue to be prevalent, particularly among impoverished populations. this study attempts to create the first health informatics model of helminthiasis in thailand. the authors investigate how a health informatics model could be used to predict the control and eradication in a national control campaign. fish-borne helminthiasis caused by opisthorchis viverrini remains a major public health problem in many parts of south-east asia, incl ... | 2016 | 27666946 |
| efficacious and safe dose of praziquantel for the successful treatment of feline reservoir hosts with opisthorchiasis. | opisthorchiasis caused by opisthorchis viverrini is a major food-borne zoonosis in greater mekong sub-region. even though campaigns discouraging the consumption of raw fish have been launched to public, the disease still remains highly endemic. the unsuccessful eradication of the disease is probably because of the persistence of the parasite in animal reservoir hosts, particularly felids. praziquantel (pzq) is the drug of choice for morbidity control of opisthorchiasis in humans and animals. how ... | 2016 | 27576001 |
| morbidity assessment of opisthorchis viverrini infection in rural laos: i. parasitological, clinical, ultrasonographical and biochemical findings. | infections with the food-borne trematode opisthorchis viverinni are common in southeast asia. in lao pdr alone, two million people are supposed to be infected. opisthorchiasis may cause severe liver disease, eventually leading to cholangiocarcinoma. the objective of this study is to assess the eating habits, complaints, symptoms, signs and ultrasonographical findings in three different areas of savannakhet province. | 2016 | 27433131 |
| single-ascending-dose pharmacokinetic study of tribendimidine in opisthorchis viverrini-infected patients. | praziquantel is the only drug available for the treatment of opisthorchis viverrini infections. tribendimidine has emerged as a potential treatment alternative; however, its pharmacokinetic (pk) properties have not been sufficiently studied to date. via two phase iia dose-finding studies, 68 o. viverrini patients were treated with 25- to 600-mg doses of tribendimidine using 50- and 200-mg tablet formulations. plasma, blood, and dried blood spots (dbs) were sampled at selected time points. the tw ... | 2016 | 27431234 |
| prevalence of opisthorchis viverrini-like fluke infection in ducks in binh dinh province, central vietnam. | following the first report of opisthorchis viverrini infection in a domestic duck in phu my district of binh dinh province, central vietnam, many other cases were observed in the province. we determined the infection rate and intensity of o. viverrini infection in ducks in 4 districts of the province. a total of 178 ducks were randomly selected from 34 farms for examination of flukes in the liver and gall bladder. an infection rate of 34.3% (range 20.7-40.4% among districts) was found; the inten ... | 2016 | 27417094 |
| opisthorchis viverrini infection among people in the border areas of three provinces, northeast of thailand. | opisthorchis viverrini is still a serious problem in northeastern and northern thailand. active surveillance is required to determine updated data for further prevention and control planning. therefore, this study aimed to examine the prevalence and risk factors for o. viverrini in three provinces, northeastern thailand. a cross- sectional survey was conducted during october 2015 to march 2016 at kaeng sanam nang district of nakhon ratchasima province, waeng noi district of khon kaen province, a ... | 2016 | 27356720 |
| infectivity and development of opisthorchis viverrini metacercariae in immunosuppressed barbonymus gonionotus fingerlings (cyprinidae). | opisthorchiasis caused by opisthorchis viverrini infection, is a serious public health problem in lao pdr, cambodia, vietnam and thailand. cyprinid fish are required for the transmission of o. viverrini metacercariae to fish eating-mammal hosts including humans. the transmission of infective metacerariae is an important event, potentially resulting in the development of cholangiocarcinoma in cases of chronic opisthorchiasis. for a better understanding of the development of o. viverrini metacerca ... | 2016 | 27338181 |
| evaluation of cholangiocarcinoma risk and its related factors in wetland geographical communities of ubon ratchathani, thailand. | wetland geographical areas have a higher incidence of opisthorchis viverrini-associated cholangiocarcinoma (cca), confirmed by data from geographic information systems, than other areas. behavioral data also indicate that people in these areas traditionally eat uncooked freshwater fish dishes, a vehicle for o. viverrini infection. the best approach to reducing cca incidence is decreasing risk factors together with behavior alteration. evaluation of cca risk and its related factors are first need ... | 2016 | 27221857 |
| characterization and differential expression analysis of toxocara canis aquaporin-1 gene. | toxocara canis is an intestinal nematode of canids with a worldwide distribution, causing an important but neglected parasitic zoonosis in humans. aquaporins (aqp) are a family of water channel proteins, which function as membrane channels to regulate water homeostasis. in this study, the coding sequence of aquaporin-1 gene of t. canis (tc-aqp-1) was cloned and characterized. the obtained tc-aqp-1 coding sequence was 933 bp in length, which predicted to encode 311 amino acids. two conserved aspa ... | 2016 | 27215210 |
| cercarial emergence patterns for opisthorchis viverrini sensu lato infecting bithynia siamensis goniomphalos from sakon nakhon province, thailand. | opisthorchis viverrini sensu lato is a food-borne trematode which is classified as a class 1 carcinogen, with infection potentially leading to cholangiocarcinoma. snails of the genus bithynia act as the first intermediate hosts and an amplifying point in the parasite life cycle. in order to investigate seasonal effect on transmission dynamics of o. viverrini in bithynia siamensis goniomphalos, cercarial emergence and output profiles were monitored at different season. a total of 4533 snails orig ... | 2016 | 27154765 |
| upregulation of endothelial nitric oxide synthase (enos) and its upstream regulators in opisthorchis viverrini associated cholangiocarcinoma and its clinical significance. | endothelial nitric oxide synthase (enos) is an isoform of the enzyme nitric oxide synthase (nos) which is constitutively expressed in endothelial cells and plays important roles in vasodilation. we previously reported the importance of enos activation in cholangiocarcinoma (cca) tissues and cell lines. the present study aims to investigate the relative abundance of enos and phosphorylated enos (p-enos) and their upstream regulators vegfr3, vegfc, epha3 and ephrin-a1, in the opisthorchis viverrin ... | 2016 | 27143607 |
| chicken igy-based coproantigen capture elisa for diagnosis of human opisthorchiasis. | diagnosis of opisthorchis viverrini infection by conventional stool examination is increasingly difficult due to the low intensity of the infection after several rounds of control programmes in endemic regions as well as coinfections with intestinal flukes. therefore sensitive and specific diagnostic test is needed. in this study, a coproantigen sandwich elisa using recombinant o. viverrini cathepsin f (rov-cf) was developed. this sandwich elisa employing chicken igy raised against rov-cf in com ... | 2016 | 27140305 |
| prevalence and factors associated with opisthorchis viverrini infection in khammouane province, lao pdr. | opisthorchis viverrini (ov) liver flukes are common parasites found in central and southern laos and constitute a major public health problem in the country. laos people continue to have the habit of extensively consuming raw or half-cooked fish which are intermediate hosts. this study aimed to study the prevalence and factors associated with ov infection in the population of thakek district, khammouane province. this cross-sectional analytic study covered 237 subjects who filled out structured ... | 2016 | 27039810 |
| plasma peptidome as a source of biomarkers for diagnosis of cholangiocarcinoma. | cholangiocarcinoma (cca) is the bile duct cancer which constitutes one of the important public health problems in thailand with high mortality rate, especially in the opisthorchis viverrini (a parasite risk factor for cca) endemic area of the northeastern region of the country. this study aimed to identify potential biomarkers from the plasma peptidome by cca patients. peptides were isolated using 10 kda cut-off filter column and the flow-through was then used as a peptidome for lc-ms/ms analysi ... | 2016 | 27039742 |
| the complete mitochondrial genome of orthocoelium streptocoelium (digenea: paramphistomidae) for comparison with other digeneans. | orthocoelium streptocoelium is a common paramphistome species parasitizing the rumen and/or reticulum of small ruminants, leading to significant losses. this study first determined the complete mitochondrial (mt) genome of o. streptocoelium. the complete mt genome of o. streptocoelium was amplified, sequenced, assembled, analysed and then compared with those of other digeneans. the entire mt genome of o. streptocoelium is 13,800 bp in length, which is smaller than those of other digeneans except ... | 2017 | 27030161 |
| opisthorchis viverrini infections and associated risk factors in a lowland area of binh dinh province, central vietnam. | opisthorchiasis caused by opisthorchis viverrini is a major public health problem in the mekong basin in south east asia. it is associated with cholangiocarcinoma, a fatal cancer of the bile duct, which is very common in some areas of thailand and lao pdr. although there is evidence of opisthorchiasis in the central and southern provinces of vietnam, data are scarce and vietnam is often not considered an opisthorchiasis endemic area in the international literature. we conducted a cross-sectional ... | 2016 | 26872984 |
| re-examination of opisthorchis viverrini in nakhon ratchasima province, northeastern thailand, indicates continued needs for health intervention. | opisthorchis viverrini infection is associated with cholangiocarcinoma particularly in the cases of chronic or re-infection. this presents a serious health problem in northeastern and northern thailand. a community base approach is required for surveillance. therefore, in a pilot project, re-examination of o. viverrini infection was conducted in the 3 districts of nakhon ratchasima province, thailand, during june and october 2015. a total of 355 participants from a 194,152 population, was select ... | 2016 | 26838215 |
| andrographolide ameliorates beta-naphthoflavone-induced cyp1a enzyme activity and lipid peroxidation in hamsters with acute opisthorchiasis. | opisthorchis viverrini (ov) infection generates oxidative stress/free radicals and is considered as a primary cause ofcholangiocarcinoma since it primarily triggers sclerosing cholangitis. | 2016 | 26817235 |
| comparison of infectivity, metacercarial burden and host mortality induced by opisthorchis viverrini sensu lato cercariae from lao pdr compared with thailand in cyprinid fish, barbonymus gonionotus. | previous studies indicate that opisthorchis viverrini sensu lato (s.l.) is a species complex with strong associations to geographical regions, i.e., specific wetlands. the present study was conducted to compare the infectivity, establishment and metacercarial burden and survival of the fish host following exposure to o. viverrini s.l. cercariae isolates from two different river wetlands. | 2016 | 26740362 |
| nanoencapsulated curcumin and praziquantel treatment reduces periductal fibrosis and attenuates bile canalicular abnormalities in opisthorchis viverrini-infected hamsters. | this study investigated the effects of nanoencapsulated curcumin (nec) and praziquantel (pzq) treatment on the resolution of periductal fibrosis (pdf) and bile canalicular (bc) abnormalities in opisthorchis viverrini infected hamsters. chronic o. viverrini infection (ov) was initially treated with either pzq (op) and subsequently treated with nec (op+nec), curcumin (op+cur) or unloaded carriers (op+carrier) daily for one month. op+nec treatment reduced the pdf by suppression of fibrotic markers ... | 2016 | 26542278 |
| diagnosis of opisthorchis viverrini infection with handheld microscopy in lao people's democratic republic. | opisthorchiasis is a neglected tropical disease, yet it is of considerable public health importance in southeast asia given the predilection for chronically infected persons to develop cholangiocarcinoma. we evaluated a handheld microscope for the diagnosis of opisthorchis viverrini in a community-based setting in lao people's democratic republic in comparison with conventional light microscopy. in stool samples collected from 104 individuals, handheld microscopy revealed a sensitivity of 70.6% ... | 2016 | 26526923 |
| bithynia siamensis goniomphalos, the first intermediate host of opisthorchis viverrini in thailand. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains as medically important problem in thailand especially in the north-eastern part. infection with this parasite can lead to cholangiocarcinoma improvement. the highest prevalence of o. viverrini infection has been found in the northeast thailand and is associated with the high incidence rate of cholangiocarcinoma. to complete the life cycle of o. viverrini, the freshwater snails namely bithynia funiculata, bithynia siamensis s ... | 2015 | 26522291 |
| trematode infection of freshwater snail, family bithyniidae in thailand. | opisthorchis viverrini is restricted to and requires for its aquatic life cycle only bithynia snail as first intermediate host but many species of cyprinid fish as second intermediate hosts. a survey in thailand of trematode infection in freshwater snails of the family bithyniidae carried out during october 2008 - july 2009 found a total of 5,492 snails, classified into ten species distributed in various geographic areas. bithyniafuniculata and gabbia pygmaea were localized to the north, b. s. g ... | 2015 | 26521513 |
| community participation in cholangiocarcinoma prevention in ubon ratchathani, thailand: relations with age and health behavior. | a high prevalence of opisthorchis viverrini infection is usually found in wetland geographical areas of thailand where people have traditional behavior of eating uncooked freshwater fish dishes which results in cholangiocarcinoma (cca) development. there were several approaches for reducing opisthorchiasis-linked cca, but the prevalence remains high. to develop community participation as a suitable model for cca prevention is, firstly, to know what factors are related. we therefore aimed to inve ... | 2015 | 26514539 |
| review and current status of opisthorchis viverrini infection at the community level in thailand. | opisthorchis viverrini is remains a public health problem in thailand, particularly in the northeast and north regions which have the highest incidences of chonalgiocarcinoma (cca). o. viverrini causes the disease opithorchiasis, and its has been classified as a group 1 biological carcinogen. humans, dogs, and cats become infected with o. viverrini by ingesting raw or undercooked fish containing infective metacercariae. the first human cases of o. viverrini infection were reported in thailand 10 ... | 2015 | 26514452 |
| human trichostrongyliasis: a hospital case series. | trichostrongylus is a common nematode found to infect livestock throughout the tropics and can cause accidental zoonosis in humans. in the lao pdr and thailand, cases of human trichostrongyliasis have been reported sporadically but clinical data are limited. we retrospectively reviewed 41 cases of trichostrongyliasis who presented to srinagarind hospital, thailand from 2005 to 2012. the diagnosis of trichostrongyliasis was made by finding their eggs in the stool of patients. of the 41 cases revi ... | 2015 | 26513921 |
| anticancer activity of atractylodes lancea (thunb.) dc in a hamster model and application of pet-ct for early detection and monitoring progression of cholangiocarcinoma. | opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) is an important cancer in the great mekong region, particularly in thailand. limitations of treatment options and the lack of an effective diagnostic tool for early detection of cca are major concerns for the control of this type of cancer. the aim of the study was to investigate anti-cca activity of the ethanolic extract of atractylodes lancea (thunb.) dc., and the applicability of positron emission tomography-computed tomography (pet ... | 2015 | 26434829 |
| combination of praziquantel and aspirin minimizes liver pathology of hamster opisthorchis viverrini infection associated cholangiocarcinoma. | opisthorchiasis is one of the major risk factors for cholangiocarcinoma (cca) in northeastern thailand. an effective drug for killing this parasite is praziquantel. recently, several reports have shown that with frequent use, praziquantel may itself be a cca risk and can cause liver cell damage from an immunopathological response after parasite death. aspirin has many properties including anti-inflammation and anti-cancer. therefore, we use of aspirin (as) and praziquantel (pz) to improve hepato ... | 2016 | 26277607 |
| development and evaluation of pcr methods based on cytochrome c oxidase subunit one (cox1) and nadh dehydrogenase subunit one gene (nad1) to detect opisthorchis viverrini in human fecal samples. | opisthorchis viverrini is highly prevalent throughout southeast asia. chronic infection of this parasite leads to cholangiocarcinoma (cca), a fatal bile duct cancer. the early and accurate detection of this parasite is very important; therefore, new pcr methods targeting cytochrome c oxidase subunit one and nadh dehydrogenase subunit one gene to detect o. viverrini in fecal specimens have been developed. ninety o. viverrini-positive human fecal samples were used in this study. the pcr sensitivit ... | 2015 | 26239798 |
| levels of 8-oxodg predict hepatobiliary pathology in opisthorchis viverrini endemic settings in thailand. | opisthorchis viverrini is distinct among helminth infections as it drives a chronic inflammatory response in the intrahepatic bile duct that progresses from advanced periductal fibrosis (apf) to cholangiocarcinoma (cca). extensive research shows that oxidative stress (os) plays a critical role in the transition from chronic o. viverrini infection to cca. os also results in the excision of a modified dna lesion (8-oxodg) into urine, the levels of which can be detected by immunoassay. herein, we m ... | 2015 | 26230769 |
| mri and (1)h mrs findings of hepatobilary changes and cholangiocarcinoma development in hamsters infected with opisthorchis viverrini and treated with n-nitrosodimethylamine. | 3 t mri and (1)h mrs were useful for quantitative investigation of the serial development of hepatobiliary changes in opisthorchis viverrini infection in hamsters, and the differential diagnosis of cholangiocacinoma (cca) development from bile duct changes and normal condition is unclear. in this study, we investigated the serial development of hepatobiliary changes and ccagenesis in o. viverrini-infected and n-nitrosodimethylamine (ndma) treated hamsters (on group) using 3 t mri and (1)h mrs an ... | 2015 | 26117689 |
| risk factors for opisthorchis viverrini infection in nong khai province, thailand. | opisthorchis viverrini (ov) infection is the main risk factor for cholangiocarcinoma and is often found in northeastern thailand. the prevalence of ov infection and the incidence of cholangiocarcinoma are major public health problems in this region. | 2015 | 26107209 |
| lectin microarray-based sero-biomarker verification targeting aberrant o-linked glycosylation on mucin 1. | glycoform of mucin 1 (muc1) in cancerous cells changes markedly with cell differentiation, and thus, qualitative detection and verification of the muc1 glycosylation changes have potential diagnostic value. we have developed an ultrasensitive method to detect the changes in cholangiocarcinoma (cc), which produces muc1, and applied it in the diagnostics development. the focused glycan analysis using 43-lectin-immobilized microarray could obtain the glycan profiles of sialylated muc1 in 5 μl of se ... | 2015 | 26091356 |
| prevalence and risk factors for infection by opisthorchis viverrini in an urban area of mahasarakham province, northeast thailand. | the aim of this cross-sectional analytic study was to investigate the prevalence and risk factors for opisthorchis viverrini (ov) infection in an urban area of northeastern thailand. the participants were 254 household representatives aged 15 years or older living in the most urbanised part of chiang yuen municipality in mahasarakham province. all participants provided stool samples which were examined using the modified kato-katz procedure, and a structured interview questionnaire was used to c ... | 2015 | 26028068 |
| seasonal transmission of opisthorchis viverrini sensu lato and a lecithodendriid trematode species in bithynia siamensis goniomphalos snails in northeast thailand. | seasonal changes play roles in the transmission success of fish-borne zoonotic trematodes (fzt). this study examined the seasonal transmission patterns of opisthorchis viverrini sensu lato (s.l.) and a virgulate cercaria (family lecithodendriidae) in the snail intermediate host, bithynia siamensis goniomphalos in northeast thailand. snail samples were collected monthly during the rainy, cool, and hot seasons during 2012-2013 to determine the prevalence and intensity of larval trematode infection ... | 2015 | 25918210 |
| a microrna profile associated with opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma. | intrahepatic cholangiocarcinoma (icc) is a highly aggressive tumor of the bile duct, and a significant public health problem in east asia, where it is associated with infection by the parasite opisthorchis viverrini. icc is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority. | 2015 | 25903557 |
| quantitative changes in tumor-associated m2 macrophages characterize cholangiocarcinoma and their association with metastasis. | the tumor microenvironment (tme) includes numerous non-neoplastic cells such as leukocytes and fibroblasts that surround the neoplasm and influence its growth. tumor-associated macrophages (tams) and cancer-associated fibroblasts (cafs) are documented as key players in facilitating cancer appearance and progression. alteration of the macrophage (cd68, cd163) and fibroblast (α-sma, fsp-1) cells in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) was here assessed using liver tissues f ... | 2015 | 25854403 |
| plasma phosphoproteome and differential plasma phosphoproteins with opisthorchis viverrini-related cholangiocarcinoma. | this study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of opisthorchis viverrini (ov)-related cholangiocarcinoma (cca). plasma phosphoproteomes from cca patients (10) and non-cca subjects (5 each for healthy subjects and ov infection) were investigated using gel-based and solution-based lc-ms/ms. phosphoproteins in plasma samples were enriched and analyzed by lc-ms/ms. strap, panther, ipath, and mev programs were applied for the ide ... | 2015 | 25735322 |
| lc-ms/ms method for the determination of two metabolites of tribendimidine, deacylated amidantel and its acetylated metabolite in plasma, blood and dried blood spots. | tribendimidine has emerged as potential alternative to praziquantel in the treatment of opisthorchis viverrini infections. to support its clinical development program, a quantitative high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) assay was developed for tribendimidine's degradation product deacylated amidantel (dadt) and its acetylated metabolite adadt. analytical sample preparation included protein precipitation for blood and plasma, and direct processing of dried ... | 2015 | 25553533 |
| chronic opisthorchis viverrini infection and associated hepatobiliary disease is associated with iron loaded m2-like macrophages. | chronic opisthorchis viverrini-induced hepatobiliary disease is associated with significant leukocyte infiltration, including activated macrophages; however, the polarization of infiltrating macrophages remains to be fully characterized. in this study, we characterized macrophage polarization and phenotype in chronic o. viverrini-induced hepatobiliary disease in humans and hamsters using gene expression and histochemical analysis. chronic o. viverrini infection and associated hepatobiliary disea ... | 2014 | 25548425 |
| anti-inflammatory and anti-periductal fibrosis effects of an anthocyanin complex in opisthorchis viverrini-infected hamsters. | the pharmacological activities of herbal extracts can be enhanced by complex formation. in this study, we manipulated cyanidin and delphinidin-rich extracts to form an anthocyanin complex (ac) with turmeric and evaluated activity against inflammation and periductal fibrosis in opisthorchis viverrini-infected hamsters. the ac was prepared from anthocyanins extracted from cobs of purple waxy corn (70%), petals of blue butterfly pea (20%) and turmeric extract (10%), resulting in an enhanced free-ra ... | 2014 | 25447758 |
| reprint of "experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus". | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of barbonymus gonionotus fish on infection success of o. vive ... | 2015 | 25446173 |
| seasonal cercarial emergence patterns of opisthorchis viverrini infecting bithynia siamensis goniomphalos from vientiane province, lao pdr. | snail intermediate hosts play a pivotal role in maintaining the life cycles of trematodes, including opisthorchis viverrini. we investigated the emergence patterns of o. viverrini cercariae infecting bithynia siamensis goniomphalos at foci in an endemic area in vientiane province, lao pdr. | 2014 | 25442515 |
| traditional herbal medicine for the control of tropical diseases. | throughout history, traditional herbal medicine has afforded a rich repository of remedies with diverse chemical structures and bioactivities against several health disorders. a common issue of herbal medicine is the limitation of information on their pharmacological activities and their active constituents. traditionally, the use of herbal medicine has been based on empirical treatment and passed on from generation to generation with information available only in local journals. this prevents s ... | 2014 | 25425945 |
| selective immunostaining of mehlis' gland of opisthorchis viverrini by antibody against rat diacylglycerol kinase. | the mehlis' gland of opisthorchis viverrini was selectively and intensely immunopositive with an antibody against rat diacylglycerol kinase gamma, and its entire structure with associated radiating processes was clearly demonstrated by immuno-light microscopy. in immuno-electron microscopy, the immunopositive processes were revealed to contain many vesicles and vacuoles and the immunoreactive materials were deposited diffusely in the cytoplasm except for the vesicular interior. the present findi ... | 2014 | 25417499 |
| gene-environment interaction involved in cholangiocarcinoma in the thai population: polymorphisms of dna repair genes, smoking and use of alcohol. | cholangiocarcinoma (cca) is the most common malignancy in a northeast thai population. smoking and alcohol drinking are associated with the production of free radical intermediates, which can cause several types of dna lesions. reduced repair of these dna lesions would constitute an important risk factor for cancer development. we therefore examined whether polymorphisms in dna base-excision repair (ber) genes, xrcc1 g399a and ogg1 c326g, were associated with cca risk and whether they modified t ... | 2014 | 25335960 |
| multiparasitism and intensity of helminth infections in relation to symptoms and nutritional status among children: a cross-sectional study in southern lao people's democratic republic. | the occurrence and spatial distribution of intestinal helminth infection in children is fairly well understood. however, knowledge on how helminth infections govern intestinal morbidity is scarce. we conducted a cross-sectional study to assess and quantify the relationship between single and multiple species helminth infection with clinical and self-reported morbidity indicators and nutritional status in champasack province, southern lao people's democratic republic (lao pdr). a random sample of ... | 2015 | 25291046 |
| prevalence and risk factors for opisthorchis viverrini infections in upper northeast thailand. | opisthorchis viverrini is an ongoing public health problem in northeast thailand. despite continuous efforts for decades by healthcare organizations to overcome this problem, infection rates remain high. to enable related personnel to identify and address the various issues effectively, a cross-sectional study was performed to investigate prevalence and risk factors for opisthorchiasis. the target group was 3,916 thai residents of northeast thailand who were 15 or over. participants were recruit ... | 2014 | 25169496 |
| analysis of the population genetics of opisthorchis viverrini sensu lato in the nam ngum river wetland, lao pdr, by multilocus enzyme electrophoresis. | a previous population genetics study of opisthorchis viverrini from a locality in an endemic area in thailand found little genetic variation over time and second intermediate fish host species. since a similar comparative analysis is not available for lao pdr, we conducted a study of o. viverrini from different endemic foci in vientiane province, lao pdr, based on spatial, temporal and fish host species. a total of 620 adult o. viverrini originating from the nam ngum river wetland were analysed ... | 2014 | 25033811 |
| risk factors for opisthorchis viverrini and minute intestinal fluke infections in lao pdr, 2009-2011. | the purpose of this report is to compare the prevalence of opisthorchis viverrini/minute intestinal fluke (ov/mif) in lao people's democratic republic according to different regions and evaluate the effect of ingestion of raw freshwater fish on the infection. demographic data, behavioral data, and symptoms were obtained, and the kato-katz method was used for the diagnosis of ov/mif. in total, 3,046 individuals were enrolled. the risk factors associated with acquisition of ov/mif were raw fish co ... | 2014 | 24980495 |
| localization of the cystogenous glands of opisthorchis viverrini cercariae. | opisthorchiasis in northeastern thailand is an important etiology of cholangiocarcinoma. to form the infectious stage, free swimming cercariae penetrate cyprinid fish, shed their tails, and then secret a cystic substance to cover their larval stage to form metacercariae in the fish body. we determined the location of the cystogenous glands in opisthorchis viverrini cercariae. the cercariae and metacercariae were obtained from the naturally infected snail host, bithynia siamensis goniomphalos and ... | 2014 | 24968667 |
| experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus. | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of barbonymus gonionotus fish on infection success of o. viver ... | 2014 | 24780189 |
| intestinal parasitic infections in hiv-infected patients, lao people's democratic republic. | hiv infection is an emerging problem in laos. we conducted the first prospective study on intestinal parasites, including opportunistic protozoa, in newly diagnosed hiv infected patients, with or without diarrhea. the aims were to describe the spectrum of infections, to determine their prevalence and to assess their associations with diarrhea, cd4 cell count, place of residence and living conditions. | 2014 | 24662743 |
| establishment of an allo-transplantable hamster cholangiocarcinoma cell line and its application for in vivo screening of anti-cancer drugs. | opisthorchis viverrini (o. viverrini) is a well-known causative agent of cholangiocarcinoma (cca) in humans. cca is very resistant to chemotherapy and is frequently fatal. to understand the pathogenesis of cca in humans, a rodent model was developed. however, the development of cca in rodents is time-consuming and the xenograft-transplantation model of human cca in immunodeficient mice is costly. therefore, the establishment of an in vivo screening model for o. viverrini-associated cca treatment ... | 2013 | 24516278 |
| distribution and abundance of opisthorchis viverrini metacercariae in cyprinid fish in northeastern thailand. | to increase public health awareness for prevention of opisthorchiasis caused by eating raw freshwater fish, the distribution and abundance of opisthorchis viverrini metacercariae (ov mc) was investigated in freshwater fish obtained from 20 provinces in northeastern thailand between april 2011 and february 2012. a cross-sectional survey was conducted on 12,890 fish consisting of 13 species randomly caught from 26 rivers, 10 dams, and 38 ponds/lakes. fish, were collected in each of the rainy and w ... | 2013 | 24516277 |
| curcumin prevents bile canalicular alterations in the liver of hamsters infected with opisthorchis viverrini. | opisthorchis viverrini infection causes inflammation and liver injury leading to periductal fibrosis. little is known about the pathological alterations in bile canaliculi in opisthorchiasis. this study aimed to investigate bile canalicular alterations in o. viverrini-infected hamsters and to examine the chemopreventive effects of curcumin on such changes. hamsters were infected with o. viverrini and one group of animals was fed with 1% dietary curcumin supplement. animals were examined during t ... | 2013 | 24516276 |
| analysis of new biomarkers for cholangiocarcinoma. | cholangiocarcinoma is one of the most serious diseases in northeast thailand, where its incidence is reported to be the highest in the world. we tried to develop a new method to detect cholangiocarcinoma in the early stages using serum proteins. we found that after fluorescent labeling of the sugar moiety of serum proteins, a new peak was identified, which might be a promising marker for cholangiocarcinoma. | 2014 | 24446393 |
| histological confirmation of periductal fibrosis from ultrasound diagnosis in cholangiocarcinoma patients. | cholangiocarcinoma (cca) has no specific clinical signs and symptoms and non-specific bio- and tumor-markers in the early disease stage. usually patients present to tertiary care with advanced disease stage. in order to detect early cases of cca that may present as a mass, dilatation of intrahepatic duct or combination, ultrasonography is accepted as a powerful imaging tool. a smaller mass or bile duct segmental dilatation requires further imaging for characterization, including computerized tom ... | 2014 | 24420706 |
| synergistic effects of isomorellin and forbesione with doxorubicin on apoptosis induction in human cholangiocarcinoma cell lines. | chemotherapy for advanced cholangiocarcinoma (cca) is largely ineffective, but innovative combinations of chemotherapeutic agents and natural compounds represent a promising strategy. in our previous studies, isomorellin and forbesione, caged xanthones isolated from garcinia hanburyi, were found to induce cell cycle arrest and apoptosis in cca cell lines. the subject of our inquiry is the synergistic effect(s) of these caged xanthones with doxorubicin on growth inhibition and apoptosis induction ... | 2014 | 25866479 |
| field survey focused on opisthorchis viverrini infection in five provinces of cambodia. | opisthorchiasis is endemic in thailand and lao people's democratic republic and constitutes a major public health problem throughout the mekong basin. although cambodia is located in the mekong basin, the status of o. viverrini infection in that country was not previously clarified. this research was conducted to document the extent and distribution of o. viverrini infection in cambodia. | 2014 | 24342554 |
| histopathological changes in tissues of bithynia siamensis goniomphalos incubated in crude extracts of camellia seed and mangosteen pericarp. | the present study was performed to observe histopathological changes in tissues of bithynia siamensis goniomphalos (gastropoda, bithyniidae) incubated in crude extract solutions of camellia (camellia oleifera) seed and mangosteen (garcinia mangostana) pericarp, and furthermore to estimate the molluscicidal effects of 2 plant substances. substantial numbers of bithyniid snails were incubated in various concentrations of 2 plant solution for 24 hr. as the positive control, snails incubated in vari ... | 2013 | 24327779 |
| sex differences in opisthorchiosis and the development of cholangiocarcinoma in syrian hamster model. | worldwide, the highest incidence of cholangiocarcinoma (cca) is found in northeast thailand, the endemic area of opisthorchis viverrini infection. cumulated clinical data revealed that the majority of cca patients are men. however, many other types of cancers are more commonly found in women. in this study, we investigated the sex differences in the development of cca, induced by o. viverrini infection and n-nitrosodimethylamine administration, in syrian hamsters. histopathology, liver function ... | 2014 | 24318666 |
| environmental determinants of opisthorchis viverrini prevalence in northeast thailand. | opisthorchis viverrini infection is an important, human, parasitic disease along the middle and lower mekong river of southeast asia. despite vast efforts in epidemiological research, the wide geographical variation in o. viverrini prevalence remains unexplained. to investigate the potential influence of village location, prevalence data recorded during the year 2009 for 90 villages in northeast thailand were analysed by a geographical information systems approach based on a digital elevation mo ... | 2013 | 24258888 |
| dna barcode identification of freshwater snails in the family bithyniidae from thailand. | freshwater snails in the family bithyniidae are the first intermediate host for southeast asian liver fluke (opisthorchis viverrini), the causative agent of opisthorchiasis. unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. this is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. because dna barcoding, t ... | 2013 | 24223896 |
| temperature dependence of opisthorchis viverrini infection in first intermediate host snail, bithynia siamensis goniomphalos. | determining of the success of a parasite's infectiveness in its snail host clearly depends on environmental conditions. temperature, one of the most influential factors impinging on metabolism of cold-blooded animals, is believed to be an important factor in parasitic infection in snails. in order to elucidate the influence of temperature, sex and size of snails on infectivity of opisthorchis viverrini to its first intermediate host, bithynia siamensis goniomphalos, 960 snails were divided into ... | 2015 | 24161535 |
| milk fat globule epidermal growth factor 8 serves a novel biomarker of opisthorchiasis-associated cholangiocarcinoma. | milk fat globule epidermal growth factor 8 (mfg-e8) is a pleiotropic secreted glycoprotein to play roles in mediating immune tolerance and homeostasis maintenance and enhancing angiogenesis. to evaluate its value as a biomarker in opisthorchiasis-associated cholangiocarcinoma (cca), the present study investigated mfg-e8 expression kinetics during the tumorigenesis in opisthorchis viverrini infection-induced cca, and demonstrated its expression in the tumor tissues of cca patients and its serum l ... | 2014 | 24122204 |
| a combination of praziquantel and the traditional medicinal plant thunbergia laurifolia on opisthorchis viverrini infection and cholangiocarcinoma in a hamster model. | cholangiocarcinoma (cca) associated by opisthorchis viverrini remains a health problem in southeast asia including thailand. at present, there is still no efficient treatment for cca. thunbergia laurifolia is a traditionally used medicinal plant; its aqueous leave extract possesses the antioxidant activity and anti-inflammatory on hamster opisthorchiasis had been reported previously. here, we demonstrate the combined effects of the t. laurifolia extract plus antihelminthic drug, praziquantel (pz ... | 2013 | 24057695 |