| [vaccination with solcotrichovac in vaginal trichomoniasis]. | | 1987 | 3274669 |
| managing trichomonal vaginitis refractory to conventional treatment with metronidazole. | three patients with vulvovaginitis caused by trichomonas vaginalis, which was refractory to conventional treatment with metronidazole are described. the t vaginalis strain isolated from one patient was resistant to metronidazole (minimum inhibitory concentration (mic) more than 100 mg/l) under aerobic conditions, although under anaerobic conditions it was as susceptible as a normal reference strain. the effect of the concomitant use of other medication and the influence of other vaginal pathogen ... | 1988 | 3278971 |
| establishing a correct diagnosis of vulvovaginal infection. | vulvovaginitis is a common problem and a prime reason for physician consultation. the organism most frequently responsible for vulvovaginal infection is candida albicans, and in a large majority of cases identification of the causative organism and diagnosis are easy to establish. recurrent or persistent infection, however, presents a perplexing diagnostic problem that usually can be solved with the use of appropriate supportive laboratory tests and awareness of the distinguishing features of th ... | 1988 | 3284366 |
| practical problems of diagnosing trichomoniasis in women. | analysis of eight groups of data collected at varying intervals during a period of seven years showed fluctuations in the sensitivity of tests to diagnose trichomoniasis in women. the best results were obtained from fresh, correctly prepared diamond's medium, feinberg-whittington's medium, and modified squires and mcfadzean's medium (which showed 82% to 94% relative sensitivity). poor results were identified consistently in commercially prepared bushby medium from one source (40% relative sensit ... | 1988 | 3290090 |
| does lactobacillus vaccine for trichomoniasis, solco trichovac, induce antibody reactive with trichomonas vaginalis? | solco trichovac is a vaccine of lactobacillus acidophilus developed for treating trichomoniasis. the efficacy of the vaccine is reportedly due to cross reacting antibody being produced in people immunised with the lactobacillus bacteria. several techniques, including enzyme linked immunosorbent, indirect immunofluorescence, immunoblot, and radioimmunoprecipitation assays were used to assess the extent of antigenic cross reactivity between lactobacillus acidophilus and trichomonas vaginalis. data ... | 1988 | 3290091 |
| trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded rna virus. | trichomonas vaginalis isolates were examined for the presence of viral double-stranded rna (dsrna) and the property of phenotypic variation. only the heterogeneous isolates composed of mab-reactive and -nonreactive organisms, as determined by indirect immunofluorescence and flow cytofluorometry, and capable of phenotypic variation possessed the dsrna. both the positive and negative phenotype subpopulations separated from the heterogeneous parent contained equal amounts of the dsrna. loss of the ... | 1987 | 3298522 |
| [the value of clinical examination, microscopy and culture for candida and trichomonas vaginalis vaginitis]. | | 1987 | 3299944 |
| the use of a highly sensitive electrophoretic method to compare the proteinases of trichomonads. | a highly sensitive electrophoretic method involving gelatin-containing polyacrylamide gels has been used to analyse trichomonad proteinases. multiple forms, optimally active at ph 5-6, were present in all four species examined, but the species could be distinguished from one another by both quantitative and qualitative differences. the intestinal parasites, trichomitus batrachorum and pentatrichomonas hominis, had lower specific activities than the urogenital parasites, trichomonas vaginalis and ... | 1987 | 3302703 |
| [indirect immunofluorescence in the serodiagnosis of trichomonas vaginalis infections]. | by using immunofluorescent technique the authors have observed that high titer circulating antibodies reacting with membrane or cytoplasmic structures of t. vaginalis occur in the majority (42/45) of women presenting because of trichomonas vaginitis. only exceptionally high titer antibodies have been detected in serum of male subjects affected by urethral trichomoniasis. low titer antibodies are found in serum of noninfected subjects and are supposed to be induced by cross reacting flagellate gr ... | 1987 | 3304346 |
| subepithelial organisms in trichomonal cervicitis. | trichomonas vaginalis is usually described as a surface-dwelling, noninvasive organism. most studies of the pathogenicity of this organism have been derived from cytologic studies of uterine cervical epithelium. we employed specific immunoperoxidase techniques that allow the identification of organisms in cytologic and histologic specimens. in a case of trichomonal cervicitis these organisms were demonstrated both on the epithelial surface and in subepithelial tissues. interpretations and implic ... | 1987 | 3311667 |
| [urethritis caused by trichomonas vaginalis in men. epidemiology]. | the interviews of sexually transmitted diseases (std) done in the dispensario "martínez anido" of madrid in the period understood between september of 1984 and september of 1985 are revised. among all of the 1,370 surveys, the trichomonal urethritis in the male, represents 2.2% of all of the urethritis and 4.6% of the nongonococcal urethritis in the male. mean age of patients was 35 years, the social-economic level was inferior than those which we observed in others std, all of the cases were he ... | 1987 | 3312865 |
| laboratory diagnosis of trichomonas vaginalis among women attending a venereal diseases control division. first report. | | 1987 | 3326100 |
| [correlation between the concentration of secretory antibodies and the distribution of vaginal flora]. | | 1987 | 3327137 |
| [evaluation of the usefulness of a lyophilized culture medium used for growing trichomonas vaginalis in the diagnosis of vaginal candidiasis]. | | 1987 | 3330720 |
| sexually transmitted diseases and sexual behavior in urban adolescent females attending a family planning clinic. | specimens from the lower genital tract of 102 sexually active urban adolescent females were tested for the common sexually transmitted diseases (stds). the results were correlated with concomitant clinical, demographic, and historic data. forty-one percent (42/102) were infected with one or more of the following organisms: chlamydia trachomatis (26/102), trichomonas vaginalis (13/102), neisseria gonorrhoeae (10/102), yeast (6/102), condyloma (5/102), and herpes simplex virus (1/44). mixed infect ... | 1988 | 3335474 |
| influence of gender in pathogenesis of trichomoniasis in congenitally athymic (nude) mice. | evaluation of abscesses appearing in male and female euthymic and athymic (nude) balb/c mice after subcutaneous injection of trichomonas vaginalis in the dorsal region showed that females were more susceptible than males. female euthymic mice, however, were more susceptible than male athymic mice, and splenectomised athymic males were more susceptible than non-splenectomised athymic males. f1 female athymic mice were the most susceptible, as their abscesses reached a peak size five days earlier ... | 1988 | 3346023 |
| [trichomonas vaginalis infection. frequency and diagnosis in women]. | | 1988 | 3353949 |
| association of coexisting vaginal infections and multiple abusers in female children with genital warts. | all 11 girls less than 12 years of age presenting to the pediatric clinic between 1980 and 1986 with genital warts were evaluated by the child protection team for possible sexual abuse. nine of the 11 girls had condylomata acuminata, and two had verruca vulgaris. ten of the 11 had historical and/or physical evidence other than the warts that confirmed sexual abuse. six girls had coexisting vaginal infections such as bacterial vaginosis and infections due to various pathogens including neisseria ... | 1988 | 3358240 |
| treatment failure in trichomonas vaginalis infections in females. i. concentrations of metronidazole in plasma and vaginal content during normal and high dosage. | concurrent estimation by high pressure liquid chromatography (hplc) of the concentrations of metronidazole in plasma and vaginal content in 12 patients with infections due to trichomonas vaginalis who did not respond to normal and high-dose treatment has shown that the two levels are closely related to each other and to the dose. individual idiosyncrasies in absorption of drug from the gut lumen or in its transfer into the vaginal content are unlikely to be the cause of treatment failure. | 1988 | 3360695 |
| trichomonal vaginitis: evaluation of serological tests and identification of immunoreactive surface peptides. | an indirect haemagglutination assay (iha) with polysaccharide and protein antigens of trichomonas vaginalis and an enzyme linked immunosorbent assay (elisa) were used to test for antibodies against t vaginalis in 58 women with trichonomal vaginitis and 48 with non-specific vaginitis. eleven antibody positive sera were used in a radioimmunoprecipitation assay (ripa) to identify surface peptides that elicited antibody responses in infected women. the serological tests were less sensitive than biol ... | 1988 | 3384427 |
| [metronidazole-resistant trichomoniasis and successful therapy following high dosage]. | a patient is reported who suffered for several months from a trichomonas vaginalis infection that was resistant to the usual low-dose treatment with 5-nitro-imidazole derivatives. following various ineffective therapeutic trials, the agent was isolated in order to determine its sensitivity to 5-nitro-imidazole. the resistance of the isolate to metronidazole was confirmed in vitro and in an animal experiment; the patient was therefore treated with high daily doses of metronidazole, 3 x 750 mg ora ... | 1988 | 3384665 |
| treatment failure in trichomonas vaginalis infections in females. ii. in-vitro estimation of the sensitivity of the organism to metronidazole. | we have developed a standard procedure to measure the susceptibility of trichomonas vaginalis to metronidazole in vitro, by controlling the state of oxygenation of the test cultures, the size of the inocula in terms of infectivity, and providing multiple tests at each drug concentration. estimates were made of the in-vitro sensitivity to metronidazole of t. vaginalis isolated from 11 randomly selected patients, all, except for three defaulters, known to have been treated successfully, and from s ... | 1988 | 3391860 |
| pathogenicity of tritrichomonas mobilensis: subcutaneous inoculation in mice. | the standard "subcutaneous mouse assay" was used to investigate the inherent pathogenicity of tritrichomonas mobilensis, an intestinal parasite of squirrel monkeys. c57b1/6 mice given subcutaneous bilateral inocula of t. mobilensis died by day 4 postinoculation with lesions too small to be measured. control mice similarly inoculated with pathogenic and nonpathogenic strains of trichomonas vaginalis survived the challenge and produced lesions on day 6 with mean volumes in agreement with previous ... | 1988 | 3418457 |
| genetic differentiation and biochemical polymorphism among trichomonads. | isoenzyme electrophoresis was used to study levels of genetic differentiation among strains and clones of trichomonas gallinae, trichomonas vaginalis, tritrichomonas foetus, tetratrichomonas gallinarum, and pentatrichomonas hominis. strain variation was found within t. gallinae, t. vaginalis, and t. foetus, however, levels of enzyme polymorphism were greater in t. gallinae than in t. vaginalis or t. foetus. isoenzyme genotypes were not a stable property of t. gallinae clones cultivated in vitro. ... | 1988 | 3418458 |
| a new metabolic labelling medium for trichomonas vaginalis and tritrichomonas foetus using 35s methionine. | a metabolic labelling medium was devised for trichomonas vaginalis and tritrichomonas foetus utilizing 35s methionine. t. vaginalis cultured for 24h in the medium took up approximately 27% of the available label and increased greater than two fold in number. counts per microgram of protein were 32,555 +/- 10% between different strains or identical strains in different labelling runs. t. foetus took up approximately 5% of the available label and increased greater than two fold in 24h. this result ... | 1986 | 3485292 |
| etiology of cervical inflammation. | we studied the relationships of selected microbial, clinical, demographic, and behavioral variables to mucopurulent cervicitis in two clinical settings, a sexually transmitted disease clinic and a student health clinic. from each clinic, we studied a group of women referred for suspected mucopurulent cervicitis and a representative sample of other women attending the clinic. after the women were stratified by patient group and summary odds ratios for all groups were obtained, mucopurulent cervic ... | 1986 | 3485379 |
| trichomonas vaginalis: electrophoretic analysis and heterogeneity among isolates due to high-molecular-weight trichomonad proteins. | the protein composition of trichomonas vaginalis isolates was evaluated using one-dimensional and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. at least two hundred intrinsically labeled and about 30 major extrinsically labeled proteins of molecular weights less than 120,000 were resolved by isoelectric focusing and electrophoresis. in general, long-term grown and fresh isolates of t. vaginalis gave similar fluorograms of the readily detectable prote ... | 1986 | 3485537 |
| tioconazole 2% cream in the treatment of trichomonas vaginalis or mixed vaginal infections. | twenty patients with documented t. vaginalis infections, six of whom were simultaneously infected with c. albicans and one with g. vaginalis, were treated topically (intravaginally) once daily with 5 g of 2% tioconazole vaginal cream (trosyd, pfizer) for 3 consecutive days in an open, non-comparative study. at the first follow-up visit (about 7 days post-treatment), 95% (19/20) of the patients were cured; 95% (18/19) of patients remained cured with respect to the baseline infection at the long-t ... | 1986 | 3485546 |
| isolation of trichomonas vaginalis: a simple diagnostic medium for use in developing countries. | | 1986 | 3485756 |
| growth and cytopathogenicity of trichomonas vaginalis in tissue cultures. | the primary purpose of this study was to identify the mammalian tissue cultures which were most suitable for investigations of the cytopathogenicity of trichomonas vaginalis. a recently isolated strain of the organism was inoculated into 15 different tissue cultures which were maintained in an appropriately modified growth medium. proliferation of the protozoon was accompanied by the progressive disintegration of cell culture monolayers. initial focal lesions consisting of detached cells and an ... | 1986 | 3486191 |
| in vitro drug susceptibility and doses of metronidazole required for cure in cases of refractory vaginal trichomoniasis. | there are currently no laboratory or clinical guidelines for the identification and treatment of disease caused by metronidazole-resistant strains of trichomonas vaginalis. fifty-three isolates of t. vaginalis from cases of refractory vaginitis in the united states (26 states) and canada were tested for aerobic and anaerobic metronidazole susceptibility, and after various dosages of metronidazole, the therapeutic outcomes were evaluated for 31 of these cases. the mean aerobic metronidazole susce ... | 1986 | 3486237 |
| ferredoxin-dependent reduction of nitroimidazole derivatives in drug-resistant and susceptible strains of trichomonas vaginalis. | the inhibitory effect of a range of nitroimidazole-derivatives on h2 production by metronidazole resistant (cdc-85) and susceptible (c1-nih) trichomonas vaginalis strains was investigated. the 2-, 4-, and 5-nitro-derivatives used had one-electron reduction potentials within the range -250 to -525 mv. nitroimidazole concentrations giving 50% inhibition of h2 production (kih2) for compounds with one-electron reduction potentials in the range -250 to -425 mv were found to be similar for both strain ... | 1986 | 3486660 |
| persistent trichomonas vaginalis infection due to a metronidazole-resistant strain. | | 1986 | 3486706 |
| incubation time, second blind passage, and cost considerations in the isolation of trichomonas vaginalis. | in a group of 247 symptomatic and asymptomatic women, 75 (30.3%) were found to harbour trichomonas vaginalis; 39 (52%) infections were detected by wet mount and 36 (48%) were detected by culture only. of the latter, 27 produces a positive culture in 3 days or less and 9 were positive, on average, at day 6. the break even cost of culture was $6.75 per patient. among 70 wet mount-negative, primary culture-negative specimens that received a blind passage, another 3 (4.2%) positive cultures were det ... | 1986 | 3487552 |
| vaginal trichomoniasis in sharkyia governorate. | | 1986 | 3487598 |
| metronidazole-resistant clinical isolates of trichomonas vaginalis have lowered oxygen affinities. | oxygen affinities of metronidazole susceptible and resistant isolates of the parasitic flagellate protozoon trichomonas vaginalis were determined by mass spectrometric methods. apparent o2km values for the respiration of non-proliferating cell suspensions were about 10-fold higher for metronidazole resistant strains than for the susceptible strains c1-nih or nyh-286. simultaneous monitoring of hydrogen evolution in the presence of increasing o2 tensions enabled apparent ki values for h2 to be de ... | 1986 | 3487729 |
| morphological studies of the cytotoxicity of trichomonas vaginalis to normal human vaginal epithelial cells in vitro. | in vitro cultured monolayers of normal human vaginal epithelial cells were incubated with axenic cultures of trichomonas vaginalis. two strains freshly isolated from patients with trichomoniasis and one strain that had been maintained in axenic culture for several years were studied. freshly isolated trichomonads showed amoeboid movements, adherence to epithelial cell surfaces, and were cytotoxic to epithelial cells in vitro. in contrast, the laboratory strain maintained for years in axenic cult ... | 1986 | 3488256 |
| [symptomatic and asymptomatic vaginitis. microbiological, clinical and therapeutic aspects]. | | 1986 | 3488870 |
| monoclonal antibody to a major glycoprotein immunogen mediates differential complement-independent lysis of trichomonas vaginalis. | an immunoglobulin g type 2a (igg2a) monoclonal antibody (mab), designated c20a3, which reacts with a highly immunogenic trichomonad membrane glycoprotein (approximately 270,000 daltons), produced complement-independent cytolysis of trichomonas vaginalis organisms. time- and temperature-dependent lysis of parasites was observed following incubation of washed, live t. vaginalis with certain concentrations of c20a3 igg. differential killing of trichomonal isolates and clones of a given isolate by c ... | 1986 | 3488964 |
| frequency and epidemiologic associations of different types of vaginitis in symptomatic women in greece. | | 1986 | 3489617 |
| the double-stranded rna in trichomonas vaginalis may originate from virus-like particles. | a linear 5.5-kilobase double-stranded rna, identified in many strains and isolates of the parasitic protozoan trichomonas vaginalis in a previous study, is found largely intact in ribonuclease-treated homogenates of the parasite. it can be pelleted with membranes from the homogenate at 12,500 x g and further purified in cscl buoyant density-gradient centrifugations. the purified sample contains the double-stranded rna as well as one major protein with an estimated molecular mass of 85 kda in nad ... | 1986 | 3489942 |
| [in vitro activity of the combination of mercurobutal and sodium lauryl sulfate on trichomonas]. | | 1986 | 3489971 |
| [transmission of trichomonas vaginalis in swimming pools?]. | trichomonas vaginalis could not be isolated from the water of a highly frequented swimming pool of the city of zurich. the protozoon t. vaginalis may show a slight degree of motility; even after 20 minutes exposure to the chlorinated water of a swimming pool; but it looses its infectivity within seconds after exposure to water of the swimming pool. it is not yet known how trichomonads are able to survive in the secretions of the vagina and on contaminated objects (fomites) in dressing rooms. | 1986 | 3490073 |
| [observation on the effect of mandelic amide on trichomonas vaginalis by transmission electron microscopy]. | | 1986 | 3490327 |
| antiprotozoal effects of some hydrazones. | antiprotozoal effects of hydrazones derived from compounds with 1 to 4 carbon atoms were studied on the model organism trichomonas vaginalis isolated from a female patient with acute urogenital trichomoniasis. | 1986 | 3490421 |
| rapid assay for immunological detection of trichomonas vaginalis. | trichomoniasis is a common sexually transmitted disease with an estimated incidence of 4 million to 8 million cases a year in the united states. the most commonly used method of diagnosis is a direct microscopic observation (wet mount) of vaginal secretions and, although both rapid and inexpensive, the sensitivity of this technique is generally 50 to 70%. we developed an indirect enzyme-linked immunosorbent assay (elisa) for the detection of trichomonas vaginalis which is both rapid and sensitiv ... | 1986 | 3490489 |
| immunoglobulin isotypes of anti-trichomonas vaginalis antibodies in patients with vaginal trichomoniasis. | studies of anti-trichomonas vaginalis antibodies in patients with vaginal trichomoniasis were undertaken in attempts to identify the predominant antibody isotype produced and to delineate clinically significant antigens. the total antibody content of serum samples from 23 patients was determined by an enzyme-linked immunosorbent assay (elisa) that employed anti-human immunoglobulin and isotype-specific antisera. the immunochemical reactivity of these antibodies was examined by western blot analy ... | 1986 | 3490492 |
| prevalence of trichomonas vaginalis in some filipino women. | vaginal specimens obtained from 1,284 hospitality girls and 87 expectant mothers were examined for trichomonas vaginalis by first examining material collected from vaginal swabs and after incubation in feinberg and whittington culture medium. twenty-four percent of the specimens examined, shortly after the cotton-tip swab was placed into 1 ml culture medium, were positive by direct microscopic examination and 37% positive following 3 to 5 days incubation at 37 degrees c. only 3 of the specimens ... | 1986 | 3491430 |
| the detection of leukocyte esterase activity in urine with a new reagent strip. | an evaluation of the ames leukostix reagent strips for the detection of leukocyte esterase activity in urine was undertaken to determine the interlot precision and between reader reliability, to compare leukostix and chemstrip ln results, and to determine if the ames leukostix reagent strip provides an alternative to, or supplement for, the microscopic detection of leukocytes. a total of 412 urines were analyzed with the ames and bio-dynamics reagent strips. the sensitivity and specificity of le ... | 1987 | 3492135 |
| an agar culture technique to quantitate trichomonas vaginalis from women. | in subjects with trichomoniasis the number of trichomonas vaginalis in vaginal discharges or secretions is unknown. the presence of t. vaginalis was evaluated in 177 consecutive female patients attending an inner city sexually transmitted disease clinic by patient history, wet mount, and broth culture. t. vaginalis was quantitated by a novel agar culture technique. of the 177 women, 86 (49%) were positive for t. vaginalis by either wet mount or culture. clinical symptoms were not reliable for ma ... | 1987 | 3492572 |
| intravenous therapy of metronidazole-resistant trichomonas vaginalis. | metronidazole resistance is an uncommon cause of treatment failure of trichomonas vaginalis. high-dose intravenous metronidazole was used successfully to treat a patient in whom repeated oral therapy with this agent had failed. metronidazole resistance was documented by aerobic cultures for susceptibility; anaerobic cultures failed to indicate resistance. metronidazole serum levels were documented. with failure of repeated oral therapy, intravenous metronidazole may be indicated if there is no e ... | 1987 | 3492696 |
| experimental transmission of trichomonas vaginalis in laboratory animals and the chemistry of vaginal exudates. | experimental transmission of trichomonas vaginalis into laboratory was carried out. the protein and glucose levels of infected vaginal exudates were also investigated. subcutaneous lesions were produced in the mice with influx of neutrophils and macrophages and sloughing of epidermal parts. rats, seem to be refractory to this infection. compared with normal persons, the protein and glucose levels were higher and richer in exudates of patients with t. vaginalis infection. | 1986 | 3493997 |
| urethral trichomoniasis in men. | three hundred twenty-five men with confirmed urethral trichomoniasis were seen at sexually transmitted diseases clinics in harare, zimbabwe, in 1983-1984. the mean age of these patients was 30.4 years. the most common symptoms were urethral discharge and urethral irritation. symptoms in 252 patients (78%) had been present for more than four weeks. only 1.5% of 5548 patients with nontrichomonal nongonococcal urethritis had had symptoms for this length of time. in most patients with trichomoniasis ... | 1987 | 3494323 |
| trichomonas species: homocysteine desulphurase and serine sulphydrase activities. | homocysteine desulphurase (ec 4.4.1.2) and serine sulphydrase (ec 4.2.1.22) activities in various lines of trichomonas vaginalis, both metronidazole resistant and sensitive, and other trichomonad species were assessed. t. vaginalis contained the highest homocysteine desulphurase and serine sulphydrase activities of all the species. although the levels of the enzyme activity in t. vaginalis isolates differed, no correlation between the activities and sensitivity to metronidazole was apparent. t. ... | 1987 | 3494628 |
| determination of estrogen and androgen receptors in trichomonas vaginalis and the effects of antihormones. | trichomonas vaginalis, a common genital pathogen, was found to possess both specific estrogen and specific androgen receptors. these 4.3 s macromolecules were proteinaceous in nature. both metronidazole-resistant and metronidazole-sensitive strains possessed both types of sex hormone-binding proteins. the estrogen receptor binding was competitively inhibited by the antiestrogen tamoxifen citrate, and the androgen binding was competitively inhibited by the antiandrogen cyoctol. the presence of th ... | 1987 | 3495180 |
| the effects of inhibitors of sulphur-containing amino acid metabolism on the growth of trichomonas vaginalis in vitro. | a range of inhibitors of enzymes catalysing the metabolism of sulphur-containing amino acids were tested for efficacy against trichomonas vaginalis in vitro. sinefungin, tubercidin, ara a, bithionol, hexachlorophene, dichlorophene and 5-azacytidine were found to be effective antitrichomonal agents. combinations of any two of these inhibitors were, in most cases, no more effective than one inhibitor used alone, but marked synergy was apparent with monothioglycerol and methionine. none of the inhi ... | 1987 | 3495529 |
| detection of trichomonas vaginalis antigen in women by enzyme immunoassay. | an enzyme immunoassay (eia) was developed for the detection of trichomonas vaginalis antigen in vaginal swabs. four hundred and eighty two women attending a sexually transmitted disease (std) clinic were tested; 44 (9.1%) were positive by culture, 32 (6.6%) were positive by wet film examination, and 54 (11.2%) were considered to be positive for trichomonal antigen by eia. taking culture as the reference method, the eia had a sensitivity of 93.2% and a specificity of 97.5%. the predictive value o ... | 1987 | 3495554 |
| therapeutic activities of nitrothiazoles against trichomonads. | certain nitrothiazole derivatives, such as niridazole (ba 32644, ambilhar), exert pronounced activities against several trichomonad strains when tested under anaerobic conditions comparable to those of the nitroimidazole derivatives. few compounds of the nitrothiazole group, however, are markedly less active in vitro. the in vivo activity of the nitrothiazole derivatives as determined in mice infected subcutaneously with t. foetus, is as good as that of metronidazole. a nitrothiazole derivative ... | 1987 | 3496102 |
| human serologic response to subcellular antigens of trichomonas vaginalis. | trichomonas vaginalis organisms were processed by differential centrifugation to yield defined subcellular fractions. the various fractions were processed by methods to enhance solubility of membranes and other components. the soluble materials were analyzed for immunogenicity by a quantitative kinetic enzyme-linked immunosorbent assay (k-elisa) and the enzyme-linked immunoelectrotransfer blot (eitb) technique using human patient serum. the principal antibodies in the human serum were directed t ... | 1987 | 3496443 |
| l-methionine catabolism in trichomonads. | trichomonas vaginalis growing in complex medium produced volatile thiols at a rate of 0.7 nmol min-1 (mg protein)-1 and the parasite suspended in pbs with l-methionine excreted volatile thiols, including methanethiol, and alpha-keto acid. cell-free extracts of the parasite also produced volatile thiols from l-methionine, at the rate of 5.4 nmol min-1 (mg protein)-1. thiol production was not detectable with living cells or cell-free extracts of tritrichomonas foetus, trichomitus batrachorum or pe ... | 1987 | 3496535 |
| activities of metronidazole and niridazole against trichomonas vaginalis clinical isolates. | the activities of niridazole and metronidazole against trichomonas vaginalis c1-nih and six isolates of clinical origin were compared using the in-vitro assay technique. under the standard anaerobic assay conditions both metronidazole and niridazole were highly effective at low concentrations against all the strains used. however niridazole, in contrast to metronidazole, was equally effective in the aerobic assay, a feature which may be exploited in the chemotherapy of patients with refractory t ... | 1987 | 3497149 |
| cell culture compared with broth for detection of trichomonas vaginalis. | trichomonas vaginalis can be grown in cell culture. we studied the growth kinetics of t. vaginalis in mccoy cell culture compared with that in a conventional broth medium (diamond tyi-s-33 medium supplemented with 10% heat-inactivated bovine serum [tyi]). in the presence of mccoy cells and two parts cell culture medium to one part tyi, a peak concentration of 2 x 10(6) to 6 x 10(6) t. vaginalis per ml was consistently achieved with inocula as low as three t. vaginalis cells per ml. without cells ... | 1987 | 3497171 |
| [comparative immunoelectrophoretic studies of total water-soluble extracts of trichomonas vaginalis, t. tenax and t. hominis]. | an antigen characterization was carried out by the method of two-dimensional immunoelectrophoresis, and on this basis the antigen community and the antigenic differences between the 3 trichomonas species parasitic in man were investigated. in the homologous antigen-antibody-systems a maximum number of precipitation curves is formed--21 in t. vaginalis and 20 each in t. tenax and t. hominis. according to our setting of the experiment t. vaginalis has 5 specific antigens in regard to t. tenax and ... | 1987 | 3497593 |
| [the mechanism of action of monofunctional and bifunctional hydrazones on trichomonas vaginalis]. | | 1987 | 3497691 |
| [in vitro effect of tinidazole in trichomonas vaginalis and trichomonas hominis]. | | 1987 | 3497735 |
| comparative study of ferredoxin-linked and oxygen-metabolizing enzymes of trichomonads. | 1. the activities of pyruvate:methyl viologen oxidoreductase (ec 1.2.7.1), hydrogenase (ec 1.18.99.1), nadh:methyl viologen oxidoreductase (ec 1.6.99.3), nadph:methyl viologen oxidoreductase (ec 1.6.99.1), nadh oxidase (ec 1.6.99.3) and nadph oxidase (ec 1.6.99.1) were determined for trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum. 2. the three trichomonad species were found to differ significantly, especially with respect to nadh oxidase and nadh:methyl viologen oxidore ... | 1987 | 3497772 |
| isolation of trichomonas vaginalis from cycloheximide-treated mccoy cells. | | 1987 | 3497805 |
| trichomonas vaginalis nyh286 phenotypic variation may be coordinated for a repertoire of trichomonad surface immunogens. | trichomonas vaginalis isolate nyh286 was fractionated with immunoglobulin g of sera from patients with trichomoniasis. subpopulations of trichomonads with phenotypes of either patient serum-immunoglobulin g reactive (ps+) or nonreactive (ps-) were obtained. flow cytofluorometry of ps+ and ps- subpopulations with a monoclonal antibody called c20a3 which reacts with a high-molecular-weight immunogen of t. vaginalis gave corresponding fluorescent (positive) and nonfluorescent (negative) phenotypes. ... | 1987 | 3497876 |
| venereal trichomoniasis: role of men. | it has been suggested that high zinc concentrations found in men may prevent trichomonas vaginalis from being established in the male reproductive tract. in this investigation t vaginalis was readily killed at concentrations of zinc that occur in the prostatic fluid of healthy men (minimum trichomonacidal concentration (mtc) of 6.4 mmol/l). t vaginalis was also shown to be killed by human prostatic extracts as well as by human seminal fluid, even when the zinc content was much lower than the mtc ... | 1987 | 3498677 |
| isolation of a stable clone of the ameboid-adherent (aa) variant of trichomonas vaginalis. | | 1987 | 3498811 |
| the dsrna of trichomonas vaginalis is associated with virus-like particles and does not correlate with metronidazole resistance. | twelve metronidazole-resistant and twelve metronidazole-susceptible strains of trichomonas vaginalis were tested for the presence of dsrna. three resistant and five susceptible strains were found to contain dsrna which indicated that metronidazole resistance does not correlate with the absence of dsrna. electron microscopy showed the homogenates of all dsrna-positive strains to contain virus-like particles 32-38 nm in diameter, while no such particles were found in the dsrna-negative strains. a ... | 1987 | 3499366 |
| [the clinical significance of trichomonas vaginalis infection in male urogenital organs]. | | 1987 | 3499538 |
| probing the active sites of aspartate: 2-oxoglutarate aminotransferases from trichomonas vaginalis and pig heart cytoplasm using substrate analogues. | 1. series of structural analogues of the substrates and products of the aspartate: 2-oxoglutarate aminotransferase reaction have been tested as reversible inhibitors of the purified aspartate aminotransferases from the protozoon trichomonas vaginalis and from pig heart cytoplasm. 2. the results highlight differences and similarities between the active site regions of the two enzymes which are relevant to a better understanding of the nature of the enzyme/substrate interactions which influence su ... | 1987 | 3500014 |
| effect of d-propranolol on growth and motility of flagellate protozoa. | propranolol inhibits sperm motility and has been considered as a spermicide contraceptive. in view of the inhibitory effects of d-propranolol on sperm flagellar activity, we have investigated its effect on motility and growth of two human flagellate, protozoan parasites. d-propranolol had a dose-dependant inhibitory effect on motility of giardia lamblia and trichomonas vaginalis with ed50s of 0.38 and 0.66 mmol/l respectively, d-propranolol also inhibited growth of both parasites with ed50s of 0 ... | 1987 | 3500164 |
| purification and characterization of pyruvate: ferredoxin oxidoreductase from the anaerobic protozoon trichomonas vaginalis. | the pyruvate: ferredoxin oxidoreductase from the anaerobic protozoon trichomonas vaginalis is an extrinsic protein bound to the hydrogenosomal membrane. it has been solubilized and purified to homogeneity, principally by salting-out chromatography on sepharose 4b. low recoveries of active enzyme were caused by inactivation by o2 and the irreversible loss of thiamin pyrophosphate. it is a dimeric enzyme of overall mr 240,000 and subunit mr 120,000. the enzyme contains, per mol of dimer, 7.3 +/- 0 ... | 1987 | 3500709 |
| [trichomonacidal drugs. 6. 2,4-dichloro derivatives of piperidino- and piperazinyl-1,3,5-triazine]. | the nucleophilic substitution of one chlorine atom in cyanuric chloride (1) by piperidine derivatives (2a-c) leads to the 6-substituted 2,4-dichloro-1,3,5-triazines (3a-c). reaction of 1 with piperazine derivatives (4a-c) yields the substituted 2,4-dichloro-6-(1-piperazinyl)-1,3,5-triazines (5a-c). structures of type 3 and 5 may be characterized by the spectroscopic data. in the mass spectra, the degradation of 3c leading to the base peak comprises the cleavage into a 2,4-dichlorotriazinyl radic ... | 1987 | 3501724 |
| a rapid method for isolation of double stranded rna. | a rapid and simple method for the isolation and purification of dsrna is presented. the crucial step of this method is the extraction of proteins and dna with acid phenol. after the extraction, only rna is left in the aqueous phase. ssrna contamination of the rna preparation can be greatly reduced when ammonium sulfate is present during the extraction. | 1987 | 3501861 |
| [action of metronidazole on the parasite trichomonas vaginalis cultured in vitro]. | | 1987 | 3501875 |
| enzyme-linked immunosorbent assay for the diagnosis of trichomoniasis in women. | an enzyme-linked immunosorbent assay-(elisa) using whole trichomonas vaginalis as antigen was developed for measurement of serum antibody to t. vaginalis. sera from six women who denied ever having had genital contact were used as the negative control. of 38 women with proved t. vaginalis infection, 25 (66%) had elevated elisa values. values were usually very stable over weeks to months of follow-up. among a matched comparison group of 38 women attending the same clinic who did not have t. vagin ... | 1987 | 3501903 |
| a comparison of various methods for the determination of viability of parasitic flagellates. | to find out a simple, rapid, accurate, and reliable viability assay for in vitro anti-protozoa drug testing, a comparison among the various methods for the determination of flagellate viability was made. micro-colony forming efficiency up to 90% for three different flagellates was obtained by the agar plate method. macro-colony forming efficiency obtained by the culture tube method was generally lower (20-45%). viability determined by trypan blue exclusion correlated closely with viability deter ... | 1987 | 3502376 |
| [cytotoxic activity of trichomonas vaginalis supernatants toward cellular monolayers]. | in the present paper we analyzed the cytopathogenic activity of 3 recently isolated strains of t. vaginalis on cellular monolayers of human and animal origin. proliferation of protozoon was accompanied by the progressive and total disintegration of cellular monolayers. supernatants obtained by incubating cellular monolayers and trichomonas or trichomonas alone showed a similar cytopathic effect. a less lytic activity was shown with supernatants containing fetal calf serum. cytopathogenicity star ... | 1986 | 3502600 |
| incidence of motile, curved anaerobic rods (mobiluncus species) in vaginal secretions. | aerobic and anaerobic cultures as well as a gram stain and wet mount preparation were made of vaginal swabs taken from various groups of women including those with vaginal discharge. the bacteria commonly found in cultures were lactobacilli, coryneforms, staphylococcus epidermidis and facultative streptococci. anaerobes were isolated from 75% (475 of 632) of specimens. the incidence of trichomonas vaginalis, candida species, gardnerella vaginalis and mobiluncus species in the five groups of wome ... | 1987 | 3502614 |
| [cervico-vaginal pathogens and contraception: microbiological observations]. | the authors relate the results about 172 vaginal and cervical swabs, in women with or without oral/local (iud) contraception, with or without vaginosis/vaginitis. gardnerella vaginalis was always prevalent; the authors observed an high correlation between cervical iud and vaginal gardnerella, more than iud and cervical chlamydia trachomatis. bacterial associations in gardnerella vaginalis (mobiluncus, obligate anaerobes) are related; incidence of candida and trichomonas vaginalis are reported; c ... | 1987 | 3508298 |
| [muco-purulent cervicitis: a frequent but little-known pathology. clinical and laboratory considerations]. | in order to assess the frequency of cervicitis, to investigate its aetiological causes and to open the debate upon this subject, the authors examined 144 not hospitalized women aging between 18 and 47. they were all in their fertile period and not pregnant. the diagnosis of mpc was given on the basis of the evaluation of the number of polymorphonuclear leukocytes (pnl) found on the endocervical smears, which had been previously coloured by the gram method: the patients with an average number of ... | 1987 | 3509731 |
| role of divalent cations, ph, cytoskeleton components and surface charge on the adhesion of trichomonas vaginalis to a polystyrene substrate. | the process of adhesion of three different strains of trichomonas vaginalis to a polystyrene substrate was analysed. the process of adhesion was dependent on the time of incubation and the ph of the phosphate-buffered solution (pbs) in which the parasites were suspended. the highest indices of adhesion were observed after an incubation time of 60 min at ph 6.6. the adhesion index increased when the parasites were incubated in the presence of culture media or when ca++ or mg++ was added to the pb ... | 1987 | 3509860 |
| in vitro inhibition of trichomonas vaginalis growth by some ortho-phenacyloxy-benzyl alcohols and their derivatives. | some phenacyl ethers of different ortho-hydroxy-benzyl alcohols and analogues have been synthesized and tested for the in vitro activity towards trichomonas vaginalis. the most active compounds had a minimum inhibitory concentration of 6.25 micrograms/ml and appeared to be of a certain interest as representative of a new type of anti-trichomonas substances not containing a nitro group. | 1987 | 3509886 |
| immunogenic proteins of trichomonas vaginalis as demonstrated by the immunoblot technique. | fifteen clinical isolates of trichomonas vaginalis were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with transfer to nitrocellulose and by immunoblots probed with human sera. all t. vaginalis isolates showed similar banding patterns by coomassie brilliant blue and silver staining of the electrophoresis gels and by amido black staining of the nitrocellulose. however, by the immunoblot technique, differences in banding patterns were noted, particularly in the high-molecu ... | 1986 | 3510170 |
| monoclonal antibodies against trichomonas vaginalis. | spleen lymphocytes obtained from mice immunized with trichomonas vaginalis atcc 30001 were fused with p3-x63-ag8-653 mouse myeloma in order to produce hybridoma-secreting antibodies against t. vaginalis associated antigens. six hybridoma cloned cell lines were established; three produced igg1, two produced igg2a, and one produced igm monoclonal antibody. these six monoclonal antibodies showed binding to seven isolated strains of t. vaginalis but did not bind to giardia lamblia. three of those mo ... | 1986 | 3514432 |
| monoclonal antibody to a major surface glycoprotein immunogen differentiates isolates and subpopulations of trichomonas vaginalis. | to produce monoclonal antibodies (mabs) to highly immunogenic membrane proteins of trichomonas vaginalis nyh286, the sera of subcutaneously infected balb/c mice were first monitored for antibody to trichomonad surface proteins. the sera possessed antibody to one major surface protein by 7 days and antibody to numerous other trichomonad membrane proteins by 4 weeks postinfection. a hybridoma was then generated that synthesized an mab, designated c20a3, which reacted to a parasite-derived glycopro ... | 1986 | 3514466 |
| an immunofluorescent study of the microtubule organization in trichomonas vaginalis using antitubulin antibodies. | the flagellated protozoon trichomonas vaginalis, parasite of the human urogenital tract, possesses a well developed microtubule system organized in highly differentiated structures. we have shown by immunoblotting that monospecific anti-sheep brain tubulin antibodies are able to react with the microtubular tubulin of t. vaginalis. these antibodies were used to study the microtubular system of t. vaginalis both in interphase and mitosis by indirect immunofluorescence. the interphase microtubular ... | 1986 | 3514904 |
| evaluation of fluorescein-conjugated monoclonal antibody test to detect chlamydia trachomatis endocervical infections in adolescent girls. | we evaluated the fluorescein-conjugated monoclonal antibody (fa) test for screening for chlamydia trachomatis endocervical infection in a general adolescent clinic. three hundred sixty-three consecutive adolescent girls, ages 13 to 20 years (mean 17.3 years) were examined. forty-five (12%) fa smears had insufficient cells. reason for visit included non-lower genital tract-related disorders in 241 (76%) girls, and lower genital tract-related disorders in 77 (24%). c. trachomatis was isolated by t ... | 1986 | 3517270 |
| immunotherapeutic effect of the lactobacillus vaccine, solco trichovac, in trichomoniasis is not mediated by antibodies cross reacting with trichomonas vaginalis. | according to the producers of the lactobacillus vaccine, solco trichovac, its therapeutic effect in trichomoniasis is achieved by antibodies that are induced by the vaccination and cross react with trichomonas vaginalis. common antigens of lactobacillus acidophilus from solco trichovac vaccine and t vaginalis were therefore sought by three different seroreactions. immune serum against lacidophilus obtained by vaccinating two healthy human volunteers and two rabbits with the original solco tricho ... | 1986 | 3522408 |
| adherence of trichomonas vaginalis to cell culture monolayers. | the in vitro adherence to wish cells of a pathogenic trichomonas vaginalis strain was studied with a method utilizing thymidine-labeled protozoa. a marked dose-related adherence was observed. glutaraldehyde fixed trichomonads were not adherent. the presence of fetal calf serum during the assay did not influence attachment. concanavalin a inhibited adherence of protozoa. complete or partial inhibition of adherence was achieved by preincubating wish cells with lactobacillus fermentum or streptococ ... | 1986 | 3527701 |
| vulvar disorders in the prepubertal female. | inspection of the vulva should be a routine part of well child care. detection of poor perineal hygiene permits the establishment of good hygiene practices, which may prevent development of vulvovaginitis. condylomata acuminata, molluscum contagiosum, herpetic vulvitis, and vulvovaginitis secondary to neisseria gonorrhoeae, gardnerella vaginalis, chlamydia trachomatis, and trichomonas vaginalis arouse suspicion of child sexual abuse, which must be addressed. atopic dermatitis, psoriasis, lichen ... | 1986 | 3529014 |
| aetiology of urinary symptoms in sexually active women. | two hundred and fifty six unselected women, 50 of whom had urinary symptoms (frequency of urination or dysuria, or both), and who were attending a department of genitourinary medicine, were investigated. the urinary symptoms were associated both with pyuria and the isolation of undoubted pathogens from midstream urine (msu) specimens. no associations were found between urinary symptoms and the isolation of neisseria gonorrhoeae or chlamydia trachomatis from the urethra or cervix; the recovery of ... | 1986 | 3533756 |
| [treatment with ofloxacin (ru 43280) of uncomplicated bacterial urethritis in males]. | ofloxacin is a new fluoroquinolone with in vitro activity against the three main urethritis-causing pathogens: i.e. neisseria gonorrhoeae, chlamydia trachomatis and ureaplasma urealyticum. 18 adult males with acute uncomplicated urethritis took 200 mg ofloxacin by mouth twice daily for 7 (non-chlamydial urethritis) or 14 (chlamydial urethritis) days. 12 patients had n. gonorrhoeae; 2 had h. parainfluenzae, 1 had e. coli and 5 had c. trachomatis. urethral cultures were obtained before treatment a ... | 1986 | 3534731 |
| an 88,000-mr giardia lamblia surface protein which is immunogenic in humans. | human anti-giardia lamblia sera specifically immunoprecipitated an 88,000-mr surface protein from radioiodinated trophozoites, establishing this protein as a potentially important immunogen in humans. a mouse monoclonal antibody (gl-1) was isolated which immunoprecipitated the same 88,000-mr surface protein recognized by the human sera. gl-1 gave uniform fluorescent staining of the cell surface and flagella of g. lamblia trophozoites from the portland 1 and wb strains as well as fresh clinical i ... | 1986 | 3536744 |
| detection of trichomonas vaginalis in vaginal specimens by direct immunofluorescence assay. | vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect trichomonas vaginalis. the organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. | 1986 | 3537000 |
| is the papanicolaou smear useful for diagnosing sexually transmitted diseases? | to examine the validity of cervical cytologic diagnosis of sexually transmitted diseases, we reviewed the literature and calculated the efficacy of the papanicolaou smear as a diagnostic technique. the predictive value of a positive test varied widely, but was generally higher for trichomonas vaginalis (0.81-1.00) than for other organisms. for chlamydia trachomatis, the predictive value of a positive test ranged from 0.40-1.00. cytologic diagnosis of sexually transmitted diseases has not been st ... | 1987 | 3554060 |
| [condylomata acuminata-associated std infections of the urethra of the male. a comparative epidemiologic study]. | urethral swabs from 322 men without urethritis, 73 of whom had venereal warts (condylomata acuminata, ca) and 249 who had none, were investigated between 1981 and 1984 by microscope and culture for the presence of neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma species, trichomonas vaginalis, garderella vaginalis, b streptococci, aerobic pathogenic bacteria and candida species. the isolation frequencies in patients with and without ca were as follows: n. gonorrho ... | 1987 | 3557979 |