| Acylhomoserine lactone production by bacteria associated with cultivated mushrooms. | The main bacterial pathogens of cultivated mushroom as well as mushroom-associated bacteria, which were isolated from Agaricus bisporus, Pleurotus ostreatus and Pleurotus eryngii mushroom niches, were evaluated for the production of N-acyl-L-homoserine lactones (AHLs) by using four bioreporters. Furthermore, identification of AHLs by LC-ESI-FTICR MS was performed on culture filtrates of selected pathogens and mushroom-associated bacteria strains, which resulted in inducing at least one of the fo ... | 2011 | 21942309 |
| effects of postharvest pulsed uv light treatment of white button mushrooms (agaricus bisporus) on vitamin d(2) content and quality attributes. | pulsed uv light (puv) was investigated as a means to rapidly increase vitamin d(2) (d(2)) content in fresh button mushrooms (agaricus bisporus). d(2) was found to increase to over 100% rda/serving following 3 pulses (1 s). following 12 pulses, d(2) began to approach a maximum concentration of 27 μg/g dw. the d(2) produced with 3 pulses decreased from 11.9 to 9.05 μg/g dw after 3 days of storage; however, d(2) levels remained nearly constant after this point throughout an 11-day shelf life study. ... | 2011 | 22132934 |
| Loosening of globular structure under alkaline pH affects accessibility of ß-lactoglobulin to tyrosinase-induced oxidation and subsequent cross-linking. | Globular proteins such as ß-lactoglobulin (BLG) are poorly accessible to enzymes. We have studied susceptibility of BLG to oxidation by Trichoderma reesei (TrTyr) and Agaricus bisporus (AbTyr) tyrosinases and subsequent intermolecular cross-linking with respect to pH-induced structural changes. We evaluated pH-induced structural changes in BLG using circular dichroism, tryptophan fluorescence and small angle X-ray scattering (SAXS) measurements, where after these results were correlated with the ... | 2011 | 22112399 |
| Antioxidant properties of extracts of wild medicinal mushroom species from Croatia. | Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric de ... | 2011 | 22135877 |
| chemical composition and nutrition value of dried cultivated culinary-medicinal mushrooms from turkey. | dietary fiber, raw fiber, fructose, glucose, sucrose, maltose, vitamin a (retinol), b1 (thiamine), b2 (riboflavin), b6 (pyridoxine), and niacin contents of dried cultivated mushroom species agaricus bisporus (white and brown), lentinus edodes, and pleurotus ostreatus were determined and evaluated for nutrient and chemical composition. assays of dried mushroom samples were carried out after the drying process. dried shiitake samples showed the highest dietary fiber and raw fiber content (23.23 +/ ... | 2011 | 22164765 |
| Homology models of four Agaricus bisporus tyrosinases. | Partially purified tyrosinase from the white button mushroom Agaricus bisporus is available commercially and is a widely used experimental model for the study of tyrosinase. The structure of an H(2)L(2) tetrameric form of the mushroom enzyme was recently determined by X-ray crystallography. In this structure the two H subunits originate from the PPO3 gene, and the two L subunits are formed by a protein of unknown function with a lectin-like fold. However, the X-ray structures and oligomeric stat ... | 2012 | 22119959 |
| differential effects of shiitake- and white button mushroom-supplemented diets on hepatic steatosis in c57bl/6 mice. | shiitake mushrooms (sms) have been used in asia for treatment and/or prevention of chronic diseases and hypercholesterolemia. previously, we observed a diet supplemented with 5% sm resulted in a twofold increase in plasma il-6 levels in dba arthritic mice. an elevation in plasma il-6 has also been implicated in the pathogenesis fatty liver disease. thus, the aim of this study was to investigate the effect of sm supplemented-diet on hepatic steatosis. in study 1, eight-week old female c57bl/6 mic ... | 2011 | 21925564 |
| biophysical properties of chitosan/sirna polyplexes: profiling the polymer/sirna interactions and bioactivity. | chitosans are naturally occurring polymers widely used in life science to mediate intracellular uptake of nucleic acids such as sirna. four chitosans of fungal origin (agaricus bisporus; molecular weights mw=44, 63, 93 and 143kda) were used in this study and profiled for size, viscosity and hydrodynamic radius using gel permeation chromatography (gpc). polyplexes made of these chitosans and sirna were developed and optimized for transfection efficacy in vitro. the characteristics of these polypl ... | 2011 | 21884740 |
| the medicinal agaricus mushroom cultivated in brazil: biology, cultivation and non-medicinal valorisation. | sun mushroom is a cultivated mushroom extensively studied for its medicinal properties for several years and literature abounds on the topic. besides, agronomical aspects were investigated in brazil, the country the mushroom comes from, and some studies focus on the biology of the fungus. this review aimed to present an overview of the non-medicinal knowledge on the mushroom. areas of commercial production and marketing trends are presented. its specific fragrance, taste, nutritional value and p ... | 2011 | 22005742 |
| Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study. | Herein, it was reported and compared the chemical composition and nutritional value of the most consumed species as fresh cultivated mushrooms: Agaricus bisporus (white and brown mushrooms), Pleurotus ostreatus (oyster mushroom), Pleurotus eryngii (King oyster mushroom), Lentinula edodes (Shiitake) and Flammulina velutipes (Golden needle mushroom). Shiitake revealed the highest levels of macronutrients, unless proteins, as also the highest sugars, tocopherols and PUFA levels, and the lowest SFA ... | 2011 | 22056333 |
| Diversity in the ability of Agaricus bisporus wild isolates to fruit at high temperature (25°C). | The button mushroom Agaricus bisporus commercially cultivated requires 16-19 °C during the fruiting period. Wild strains are also present in natural habitat, and in light of their wide range of geographic distribution reported, from boreal region to tropical region, questions on the development adaptation to temperature arose. Isolates from various geographic areas were screened for their ability to fruit at higher temperature (FHT ability) than commercial cultivars. The FHT trait discriminated ... | 2011 | 22036296 |
| First Preliminary Report on Isolation and Characterization of Novel Acinetobacter spp. in Casing Soil Used for Cultivation of Button Mushroom, Agaricus bisporus (Lange) Imbach. | Despite evaluation of large number of agroindustrial wastes for their use as casing material for Agaricus bisporus (Lange) Imbach cultivation, scant attention has been given to the importance of biological properties of casing materials. In the present study, an attempt was made to characterize the bacterial flora in casing layer, namely, Farm Yard Manure (FYM) and Spent Mushroom Substrate/spent compost (SMS/SC) (FYM+SC, 3?:?1) and FYM and Vermi Compost (VC) (FYM+VC, 3?:?1), employing partial 16 ... | 2011 | 22007222 |
| Long-Distance Translocation of Protein during Morphogenesis of the Fruiting Body in the Filamentous Fungus, Agaricus bisporus. | Commercial cultivation of the mushroom fungus, Agaricus bisporus, utilizes a substrate consisting of a lower layer of compost and upper layer of peat. Typically, the two layers are seeded with individual mycelial inoculants representing a single genotype of A. bisporus. Studies aimed at examining the potential of this fungal species as a heterologous protein expression system have revealed unexpected contributions of the mycelial inoculants in the morphogenesis of the fruiting body. These contri ... | 2011 | 22163014 |
| Dietary intake of Agaricus bisporus white button mushroom accelerates salivary immunoglobulin A secretion in healthy volunteers. | OBJECTIVE: Secretory immunoglobulin A (SIgA) acts as the first line of adaptive humoral immune defense at mucosal surfaces. A lack of SIgA or the inability to produce antigen-specific SIgA can lead to an increased risk of infections. Dietary intake may improve mucosal immunity by accelerating SIgA secretion. This study investigated the effect of dietary intake of Agaricus bisporus white button mushroom (WBM) on salivary IgA (sIgA) secretion in healthy subjects. METHODS: Twenty-four healthy volun ... | 2011 | 22113068 |
| kinetics and thermodynamics of the thermal inactivation of polyphenol oxidase in an aqueous extract from agaricus bisporus. | the kinetics and thermodynamics of the thermal inactivation of polyphenol oxidase (ppo) in an aqueous extract from mushroom agaricus bisporus (j.e. lange) imbach was studied, using pyrocatechol as a substrate. optimal conditions for enzymatic studies were determined to be ph 7.0 and 35-40 °c. the kinetics of ppo-catalyzed oxidation of pyrocatechol followed the haldane model with an optimum substrate concentration of 20 mm. thermal inactivation of ppo was examined in more detail between 50 and 73 ... | 2011 | 22148350 |
| direct electrochemistry of dopamine on gold-agaricus bisporus laccase enzyme electrode: characterization and quantitative detection. | direct electrochemistry of a new laccase enzyme immobilized on gold and its application as a biosensor for dopamine (da) are investigated by voltammetry and electrochemical impedance spectroscopy. the sensor demonstrated a redox adsorption behavior with e(0')=+180mv vs. ag/agcl for immobilized agaricus bisporus laccase (lacab) enzyme. the mpa platform was assembled on au with and without utilization of ultrasounds. excellent results were obtained by using the enzyme electrode fabricated based on ... | 2012 | 22137204 |
| the bioavailability of ergothioneine from mushrooms (agaricus bisporus) and the acute effects on antioxidant capacity and biomarkers of inflammation. | background: ergothioneine (et) is a sulfur containing amino acid that functions as an antioxidant. mushrooms are a primary source of et containing from 0.4 to 2.0mg/g (dry-weight). the bioavailability of et from mushrooms in humans remains unclear. objective: we evaluated the bioavailability of et in healthy men (n=10) in a pilot study, using a randomized, cross-over, dose-response, postprandial time-course design, conducted at the general clinical research center at pennsylvania state universit ... | 2011 | 22230474 |
| evaluation of indigenous potent mushroom growth promoting bacteria (mgpb) on agaricus bisporus production. | mushrooms such as agaricus bisporus, are cultivated for food worldwide. fruit body initiation in agaricus bisporus is a phase change from the vegetative to the reproductive stage which depends on the presence of a casing layer with particular physical, chemical and microbiological properties. the phase change is achieved practically by environmental manipulation and the presence of naturally occurring bacteria such as pseuodomonas putida. in this study, 274 individual bacterial isolates were col ... | 2011 | 22806784 |
| storage and preservation of temperate mushroom cultures, agaricus bisporus and pleurotus florida. | two temperate mushroom cultures namely agaricus bisporus (u-3) and pleurotus florida (pau-5) were evaluated for their physiological (linear growth and biomass production), biochemical (β-1,4 endoglucanase production) and fruiting behaviour after preservation in 10% (v/v) glycerol and storage at room temperature (25-35°c), -20°c and -196°c for 6 months with the objective of establishing the recovery/changes in these fungi after storage. studies indicated that the viability and recovery of a. bisp ... | 2011 | 22654171 |
| the cultivation of the mushroom agaricus bisporus (champignon): micro-organisms and preservability. | cultivation of agaricus bisporus mushrooms requires the use of substrates that are potentially dangerous from the microbiological point of view, such as chicken and horse manure. microorganisms can pose risks to consumers and workers, and generate lower profits. packaging of fresh mushrooms with impermeable films is used to extend their shelf life but creates anaerobic and humidity conditions that could favour the growth of microorganisms such as listeria monocytogenes and clostridium botulinum. ... | 2011 | 22508614 |
| bioremediation of fungicides by spent mushroom substrate and its associated microflora. | experiments were conducted both under in vitro and in situ conditions to determine the biodegradation potential of button mushroom spent substrate (sms) and its dominating microbes (fungi and bacteria) for carbendazim and mancozeb, the commonly used agricultural fungicides. during 6 days of incubation at 30 ± 2°c under broth culture conditions, highest degradation of carbendazim (17.45%) was recorded with b-1 bacterial isolate, while highest degradation of mancozeb (18.05%) was recorded with tri ... | 2011 | 22282605 |
| extraction optimization and bioactivity of exopolysaccharides from agaricus bisporus. | response surface methodology was used to optimize the extraction parameters of eps produced by agaricus bisporus mj-0811 in submerged culture. the optimal levels for ethanol concentration (85%, v/v), ph (8) and precipitation time (22 h) were determined, and eps production was estimated at 2.71 g/l. the actual yield of eps under these conditions was 2.69 g/l. in addition, the antioxidant activity of eps was investigated by measuring its scavenging ability on superoxide radicals and hydroxyl radic ... | 2012 | 23399195 |
| capacity for colonization and degradation of horse manure and wheat-straw-based compost by different strains of agaricus subrufescens during the first two weeks of cultivation. | the potential of agaricus subrufescens strains to colonize and transform horse manure and wheat-straw-based mushroom compost under the physico-chemical conditions typically used for agaricus bisporus was assessed. lignocellulolytic activities, h2o2 production and substrate transformation (assessed via cp/mas nmr of (13)c) for certain a. subrufescens strains were similar or even greater than those obtained for an a. bisporus strain used as control. moreover, the functional diversity of the microb ... | 2012 | 23357087 |
| the first report on mushroom green mould disease in croatia. | green mould disease, caused by trichoderma species, is a severe problem for mushroom growers worldwide, including croatia. trichoderma strains were isolated from green mould-affected agaricus bisporus (button or common mushroom) compost and pleurotus ostreatus (oyster mushroom) substrate samples collected from croatian mushroom farms. the causal agents of green mould disease in the oyster mushroom were t. pleurotum and t. pleuroticola, similar to other countries. at the same time, the pathogen o ... | 2012 | 23334043 |
| ultrasonic-assisted extraction and in vitro antioxidant activity of polysaccharides from agaricus bisporus. | to optimize ultrasonic-assisted extraction parameters of polysaccharides from agaricus bisporus and evaluate antioxidant activities of a. bisporus polysaccharides. | 2012 | 23320369 |