Publications

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phenotypic and molecular diversity of meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation.meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen candida guilliermondii, which causes about 2% of the human blood infections. this yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. the question if isolates from different sources are physiologically and genetically similar, or if the v ...201525791010
aromatic components produced by non-saccharomyces cerevisiae derived from natural fermentation of grape.aromatic components are important functional products during the wine fermentation process. in the current study, nine strains (y10, y5, y21, y2, y19, y16, y3, y13 and y4) of non-saccharomyces were isolated from cabernet sauvignon grape wine. aromatic components from cabernet sauvignon-fermented wine were determined the phylogenetic evolution status of different non-saccharomyces based on 26s rdna and d1/d2 sequence analysis and analysed by a gas chromatography-mass spectrometry analysis, and th ...201525677508
assessment of the factors contributing to the growth or spoilage of meyerozyma guilliermondii in organic yogurt: comparison of methods for strain differentiation.in this work we analyze the spoiling potential of meyerozyma guilliermondii in yogurt. the analysis was based on contaminated samples sent to us by an industrial laboratory over two years. all the plain and fruit yogurt packages were heavily contaminated by yeasts, but only the last ones, containing fermentable sugars besides lactose, were spoiled by gas swelling. these strains were unable to grow and ferment lactose (as the type strain); they did grow on lactate plus galactose, fermented glucos ...201527682098
enhanced expression of the codon-optimized exo-inulinase gene from the yeast meyerozyma guilliermondii in saccharomyces sp. w0 and bioethanol production from inulin.in the present study, after the exo-inulinase gene inu1 from meyerozyma guilliermondii was optimized according to the codon usage bias of saccharomyces cerevisiae, both the optimized gene inu1y and the native gene inu1 were ligated into the homologous integration expression vector pmirsc11 and expressed in saccharomyces sp. w0. it was determined that the inulinase activity of the recombinant yeast y13 with the optimized gene inu1y was 43.84 u/ml, which was obviously higher than that (31.39 u/ml) ...201425236803
disrupting the methionine biosynthetic pathway in candida guilliermondii: characterization of the met2 gene as counter-selectable marker.candida guilliermondii (teleomorph meyerozyma guilliermondii) is an ascomycetous species belonging to the fungal ctg clade. this yeast remains actively studied as a result of its moderate clinical importance and most of all for its potential uses in biotechnology. the aim of the present study was to establish a convenient transformation system for c. guilliermondii by developing both a methionine auxotroph recipient strain and a functional met gene as selection marker. we first disrupted the met ...201424700391
species of candida as a component of the nasal microbiota of healthy horses.respiratory infections are a common problem among equines and occur with variable rates of morbidity and mortality. although some fungal species are considered primary agents of respiratory tract infections in several mammals, their relevance in respiratory diseases of equines is frequently neglected. in the present study, we performed an active search for candida spp. in the nasal cavity of horses. the presence of candida spp. was investigated through the use of nasal swabs that were streaked o ...201323651178
candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics.candida guilliermondii (teleomorph meyerozyma guilliermondii) is an ascomycetous species belonging to the saccharomycotina ctg clade which has been studied over the last 40 years due to its biotechnological interest, biological control potential and clinical importance. such a wide range of applications in various areas of fundamental and applied scientific research has progressively made c. guilliermondii an attractive model for exploring the potential of yeast metabolic engineering as well as ...201323616192
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