| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| candida utilis and chlorella vulgaris counteract intestinal inflammation in atlantic salmon (salmo salar l.). | intestinal inflammation, caused by impaired intestinal homeostasis, is a serious condition in both animals and humans. the use of conventional extracted soybean meal (sbm) in diets for atlantic salmon and several other fish species is known to induce enteropathy in the distal intestine, a condition often referred to as sbm induced enteropathy (sbmie). in the present study, we investigated the potential of different microbial ingredients to alleviate sbmie in atlantic salmon, as a model of feed-i ... | 2013 | 24386162 |
| assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for cbp ethanol production. | many microorganisms possess enzymes that can efficiently degrade lignocellulosic materials, but do not have the capability to produce a large amount of ethanol. thus, attempts have been made to transform such enzymes into fermentative microbes to serve as hosts for ethanol production. however, an efficient host for a consolidated bioprocess (cbp) remains to be found. for this purpose, a synthetic biology technique that can transform multiple genes into a genome is instrumental. moreover, a strat ... | 2013 | 23374631 |
| a new search for thermotolerant yeasts, its characterization and optimization using response surface methodology for ethanol production. | the progressive rise in energy crisis followed by green house gas (ghg) emissions is serving as the driving force for bioethanol production from renewable resources. current bioethanol research focuses on lignocellulosic feedstocks as these are abundantly available, renewable, sustainable and exhibit no competition between the crops for food and fuel. however, the technologies in use have some drawbacks including incapability of pentose fermentation, reduced tolerance to products formed, costly ... | 2015 | 26388844 |
| diversity of fungal flora in raw milk from the italian alps in relation to pasture altitude. | the present paper explores the diversity of mycobiota inhabiting raw milk sampled at different altitudes (1400 m, 1800 m, 2200 m) from cows grazing alpine pastures of valle d'aosta (north-western italian alps). to this aim, multilocus sequencing was performed at barcodes commonly used for fungal identification (its1, d1/d2 domains of the 26s rrna gene, and part of the β-tubulin gene). a total of 31 species were detected, most of them yeasts, followed by moulds and by 2 sequences of macroscopic f ... | 2013 | 24024093 |
| feasibility of biohydrogen production from industrial wastes using defined microbial co-culture. | the development of clean or novel alternative energy has become a global trend that will shape the future of energy. in the present study, 3 microbial strains with different oxygen requirements, including clostridium acetobutylicum atcc 824, enterobacter cloacae atcc 13047 and kluyveromyces marxianus 15d, were used to construct a hydrogen production system that was composed of a mixed aerobic-facultative anaerobic-anaerobic consortium. the effects of metal ions, organic acids and carbohydrate su ... | 2015 | 25943991 |
| the evaluation of kefir pure culture starter: liquid-core capsule entrapping microorganisms isolated from kefir grains. | the main purpose of this study was to develop a pure culture starter for producing kefir. in order to accomplish starter recycling, yeasts (kluyveromyces marxianus strain, pichia kudriavzevii clone), lactic acid bacteria (lactobacillus kefiri strain f4aa, lactobacillus kefiri strain nm131-7, lactobacillus kefiri strain nm132-3, lactobacillus kefiri strain nm180-3, respectively), and acetic acid bacteria (acetobacter lovaniensis strain) were entrapped in liquid core capsules based on the distribu ... | 2016 | 26924844 |
| short communication: evaluation of the microbiota of kefir samples using metagenetic analysis targeting the 16s and 26s ribosomal dna fragments. | milk kefir is produced by fermenting milk in the presence of kefir grains. this beverage has several benefits for human health. the aim of this experiment was to analyze 5 kefir grains (and their products) using a targeted metagenetic approach. of the 5 kefir grains analyzed, 1 was purchased in a supermarket, 2 were provided by the ministry of agriculture (namur, belgium), and 2 were provided by individuals. the metagenetic approach targeted the v1-v3 fragment of the 16s ribosomal (r)dna for the ... | 2015 | 25828663 |
| genomic analyses of thermotolerant microorganisms used for high-temperature fermentations. | environmental adaptation is considered as one of the most challenging subjects in biology to understand evolutionary or ecological diversification processes and in biotechnology to obtain useful microbial strains. temperature is one of the important environmental stresses; however, microbial adaptation to higher temperatures has not been studied extensively. for industrial purposes, the use of thermally adapted strains is important, not only to reduce the cooling expenses of the fermentation sys ... | 2016 | 26566045 |
| the effect of lactic acid bacteria on cocoa bean fermentation. | cocoa beans (theobroma cacao l.) are the raw material for chocolate production. fermentation of cocoa pulp by microorganisms is crucial for developing chocolate flavor precursors. yeasts conduct an alcoholic fermentation within the bean pulp that is essential for the production of good quality beans, giving typical chocolate characters. however, the roles of bacteria such as lactic acid bacteria and acetic acid bacteria in contributing to the quality of cocoa bean and chocolate are not fully und ... | 2015 | 25889523 |
| yeasts are essential for cocoa bean fermentation. | cocoa beans (theobroma cacao) are the major raw material for chocolate production and fermentation of the beans is essential for the development of chocolate flavor precursors. in this study, a novel approach was used to determine the role of yeasts in cocoa fermentation and their contribution to chocolate quality. cocoa bean fermentations were conducted with the addition of 200ppm natamycin to inhibit the growth of yeasts, and the resultant microbial ecology and metabolism, bean chemistry and c ... | 2014 | 24462702 |
| influencing cocoa flavour using pichia kluyveri and kluyveromyces marxianus in a defined mixed starter culture for cocoa fermentation. | the potential impact of aromatic and pectinolytic yeasts on cocoa flavour was investigated using two defined mixed starter cultures encompassing strains of pichia kluyveri and kluyveromyces marxianus for inoculating cocoa beans in small scale tray fermentations. samples for microbial and metabolite analysis were collected at 12-24 hour intervals during 120 h of fermentation. yeast isolates were grouped by (gtg)5-based rep-pcr fingerprinting and identified by sequencing of the d1/d2 region of the ... | 2013 | 23866910 |
| microbial diversity and dynamics during the production of may bryndza cheese. | diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ... | 2014 | 24291178 |
| occurrence of mycotoxins and yeasts and moulds identification in corn silages in tropical climate. | this study was aimed to identify yeasts and moulds as well as to detect mycotoxin in corn silages in southern minas gerais, brazil. | 2016 | 26787003 |
| thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production. | nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at brazilian cerrado and identified up to species level on the basis of the gene sequencing of 5.8s-its and 26s ribosomal dna regions. four species were identified: kluyveromyces marxianus, aspergillus niger, aspergillus sydowii and aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. among them, th ... | 2017 | 26413077 |
| [inulinases from various producers: the features of their permolecular organization]. | the structural organization of inulinases from yeasts, fungi, and plants are researched. for studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. it is shown that inulinases from kluyveromyces marxianus and aspergillus niger form geterodimers and inulinases from tubers of helianthus tuberosus are present as both dimers an ... | 2014 | 25272747 |
| direct fermentation of raw starch using a kluyveromyces marxianus strain that expresses glucoamylase and alpha-amylase to produce ethanol. | raw starch and raw cassava tuber powder were directly and efficiently fermented at elevated temperatures to produce ethanol using the thermotolerant yeast kluyveromyces marxianus that expresses α-amylase from aspergillus oryzae as well as α-amylase and glucoamylase from debaryomyces occidentalis. among the constructed k. marxianus strains, yrl 009 had the highest efficiency in direct starch fermentation. raw starch from corn, potato, cassava, or wheat can be fermented at temperatures higher than ... | 2014 | 24478139 |
| investigation on culturable microflora in tibetan kefir grains from different areas of china. | four samples of tibetan kefir grains (tk-zjuj 01-04) from tibet and surrounding areas were investigated via phenotypic and genotypic methods to compare and analyze the diversity of culturable microflora among different origins. as a result, 4 genera of microorganisms from tk-zjuj01: bacillus subtilis (2.9 × 10(7) cfu/ml), lactococcus lactis (8.2 × 10(7) cfu/ml), kluyveromyces marxianus (3.0 × 10(6) cfu/ml), saccharomyces cerevisiae (9.0 × 10(6) cfu/ml); 4 genera from tk-zjuj02: lactobacillus kef ... | 2012 | 22860591 |
| antifungal properties of hypericin, hypericin tetrasulphonic acid and fagopyrin on pathogenic fungi and spoilage yeasts. | the role of hypericin-mediated photodynamic antimicrobial properties on pathogenic fungi and photodynamic therapy for human cancer cells is known. antifungal properties of hypericum perforatum l. (hypericaceae) and fagopyrum esculentum moench. (polygonaceae) extracts were also studied. the different polarities of solvents can cause complication in the identification of antifungal effects of separate biologically active compounds. in recent experimental work, we compared antifungal properties of ... | 2016 | 27564138 |
| yeast diversity in a traditional french cheese "tomme d'orchies" reveals infrequent and frequent species with associated benefits. | this study is aimed at unrevealing the yeast diversity of handmade cheese, tomme d'orchies, produced and marketed in the north of france. a total of 185 yeast colonies were isolated from the surface and core of this cheese. from these, 80 morphologically different colonies were selected and subjected to rep-pcr analysis. the isolates were clustered into six distinct groups based on their dna fingerprints. from each group, at least 30% of isolates were selected and identified to species level by ... | 2015 | 26338133 |
| two missense mutations, e123q and k151e, identified in the erg11 allele of an azole-resistant isolate of candida kefyr recovered from a stem cell transplant patient for acute myeloid leukemia. | we report on the first cloning and nucleotide sequencing of an erg11 allele from a clinical isolate of candida kefyr cross-resistant to azole antifungals. it was recovered from a stem cell transplant patient, in an oncohematology unit exhibiting unexpected high prevalence of c. kefyr. two amino acid substitutions were identified: k151e, whose role in fluconazole resistance was already demonstrated in candida albicans, and e123q, a new substitution never described so far in azole-resistant candid ... | 2014 | 24936404 |
| nomimicin, a new spirotetronate-class polyketide from an actinomycete of the genus actinomadura. | nomimicin (1), a new spirotetronate-class polyketide, was isolated from the culture broth of an actinomycete of the genus actinomadura. its structure was established by spectroscopic methods, and the absolute configuration was determined by a combination of noesy experiment, j-based configuration analysis and the modified mosher method. nomimicin (1) showed antimicrobial activity against micrococcus luteus, candida albicans and kluyveromyces fragilis. | 2012 | 22534651 |
| uncommon opportunistic yeast bloodstream infections from qatar. | eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ... | 2014 | 24934803 |
| microbial biogeography of the transnational fermented milk matsoni. | the fermented milk matsoni is a traditional, national food product of both georgia and armenia. little is known about the effects of biogeography and milk type on the microbial biodiversity of matsoni or the fungal composition of matsoni fermentations. high-throughput marker-gene sequencing was used to survey the bacterial and fungal communities of matsoni from different milk types and regions throughout armenia and georgia. results demonstrate that both production region and milk type influence ... | 2015 | 25998810 |
| phytase-producing capacity of yeasts isolated from traditional african fermented food products and phypk gene expression of pichia kudriavzevii strains. | phytate is known as a strong chelate of minerals causing their reduced uptake by the human intestine. ninety-three yeast isolates from traditional african fermented food products, belonging to nine species (pichia kudriavzevii, saccharomyces cerevisiae, clavispora lusitaniae, kluyveromyces marxianus, millerozyma farinosa, candida glabrata, wickerhamomyces anomalus, hanseniaspora guilliermondii and debaryomyces nepalensis) were screened for phytase production on solid and liquid media. 95% were a ... | 2015 | 25910031 |
| yeast dynamics during spontaneous fermentation of mawè and tchoukoutou, two traditional products from benin. | mawè and tchoukoutou are two traditional fermented foods largely consumed in benin, west africa. their preparations remain as a house art and they are the result of spontaneous fermentation processes. in this study, dynamics of the yeast populations occurring during spontaneous fermentations of mawè and tchoukoutou were investigated using both culture-dependent and -independent approaches. for each product, two productions were followed. samples were taken at different fermentation times and yea ... | 2013 | 23756236 |
| improving xylitol production at elevated temperature with engineered kluyveromyces marxianus through over-expressing transporters. | three transporter genes including kluyveromyces marxianus aquaglyceroporin gene (kmfps1), candida intermedia glucose/xylose facilitator gene (cigxf1) or glucose/xylose symporter gene (cigxs1) were over-expressed in k. marxianus yzj017 to improve xylitol production at elevated temperatures. the xylitol production of yzj074 that harbored cigxf1 was improved to 147.62g/l in erlenmeyer flask at 42°c. in fermenter, 99.29 and 149.60g/l xylitol were produced from 99.55 and 151.91g/l xylose with product ... | 2015 | 25465792 |
| manganese tolerance in yeasts involves polyphosphate, magnesium, and vacuolar alterations. | basidiomycetous and ascomycetous yeast species were tested for manganese tolerance. basidiomycetous cryptococcus humicola, cryptococcus terricola, cryptococcus curvatus and ascomycetous candida maltosa, kluyveromyces marxianus, kuraishia capsulata, lindnera fabianii and sacharomyces cerevisiae were able to grow at manganese excess (2.5 mmol/l), while the growth of basidiomycetous rhodotorula bogoriensis was completely suppressed. the lag phase duration increased and the exponential growth rate d ... | 2016 | 26646947 |
| cloning and characterization of an inulinase gene from the marine yeast candida membranifaciens subsp. flavinogenie w14-3 and its expression in saccharomyces sp. w0 for ethanol production. | the inu1 gene encoding an exo-inulinase from the marine-derived yeast candida membranifaciens subsp. flavinogenie w14-3 was cloned and characterized. it had an open reading frame of 1,536 bp long encoding an inulinase. the coding region of it was not interrupted by any intron. the cloned gene encoded 512 amino acid residues of a protein with a putative signal peptide of 23 amino acids and a calculated molecular mass of 57.8 kda. the protein sequence deduced from the inulinase gene contained the ... | 2015 | 25432793 |
| diversity of culturable yeasts in phylloplane of sugarcane in thailand and their capability to produce indole-3-acetic acid. | yeasts were isolated by the enrichment technique from the phylloplane of 94 samples of sugarcane leaf collected from seven provinces in thailand. all sugarcane leaf samples contained yeasts and 158 yeast strains were obtained. on the basis of the d1/d2 domain of the large subunit rrna gene sequence analysis, 144 strains were identified to 24 known species in 14 genera belonging to the ascomycota viz. candida akabanensis, candida dendronema, candida mesorugosa, candida michaelii, candida nivarien ... | 2014 | 24442819 |
| the predominance, biodiversity and biotechnological properties of kluyveromyces marxianus in the production of pecorino di farindola cheese. | pecorino di farindola is a handicraft cheese made by farmers on small scale using raw ewes' milk and pig rennet. in this study, yeast consortia were evaluated during pecorino di farindola making and ripening. molecular identification of 156 isolates was achieved by a combination of pcr-rflp of the 5.8s its rrna region and sequencing of the d1/d2 domain of the 26s rrna gene. kluyveromyces marxianus was the predominant species, while other species (pichia kudriavzevii, candida parapsilosis, candid ... | 2014 | 25038503 |
| determination of the total phenolic and anthocyanin contents and antimicrobial activity of viburnum opulus fruit juice. | viburnum opulus is a plant with fruits that are rich in biologically active substances, making it valuable to the food and pharmaceutical industries. here, we present our study of the total phenolic and anthocyanin contents and antimicrobial activity in the fruit juice of six v. opulus l. accessions. the cultivar 'krasnaya grozd' was notable for its exceptionally large amount of total phenolics, 1168 mg/100 g, with anthocyanins comprising 3-5 % of the total phenolic content. the evaluation of th ... | 2012 | 22865031 |
| effect of evolutionary adaption on xylosidase activity in thermotolerant yeast isolates kluyveromyces marxianus nire-k1 and nire-k3. | efficient use of xylose along with glucose is necessary for the economic production of lignocellulosic based biofuels. xylose transporters play an important role in the microorganisms for efficient utilization of xylose. in the present study, a novel method has been developed for a rapid assay of xylose transport activity in the xylose-utilizing isolates and other known yeasts. an assay was conducted to compare the activity of β-xylosidase using p-nitrophenyl-β-d-xylopyranoside (pnpx) in the int ... | 2016 | 27008328 |
| characterization of osmotolerant yeasts and yeast-like molds from apple orchards and apple juice processing plants in china and investigation of their spoilage potential. | yeasts and yeast-like fungal isolates were recovered from apple orchards and apple juice processing plants located in the shaanxi province of china. the strains were evaluated for osmotolerance by growing them in 50% (w/v) glucose. of the strains tested, 66 were positive for osmotolerance and were subsequently identified by 26s or 5.8s-its ribosomal rna (rrna) gene sequencing. physiological tests and rapd-pcr analysis were performed to reveal the polymorphism of isolates belonging to the same sp ... | 2015 | 26130165 |
| lipid production from hemicellulose and holocellulose hydrolysate of palm empty fruit bunches by newly isolated oleaginous yeasts. | palm empty fruit bunches (efbs) are abundant lignocellulosic wastes from palm oil mills. they are potential sources of sugars which can be converted to microbial lipids by oleaginous yeasts. to produce sugars from efb, two-step and one-step hydrolysis reactions were performed. in the first step, the use of diluted sulfuric acid (0.5 % w/v) hydrolyzed hemicelluloses and released mainly pentoses, and in the second step of hydrolysis of residual pulp using 2.5 % (w/v), sulfuric acid released more h ... | 2015 | 26026262 |
| role of hydrophobicity in adhesion of wild yeast isolated from the ultrafiltration membranes of an apple juice processing plant. | the role of cell surface hydrophobicity in the adhesion to stainless steel (ss) of 11 wild yeast strains isolated from the ultrafiltration membranes of an apple juice processing plant was investigated. the isolated yeasts belonged to four species: candida krusei (5 isolates), candida tropicalis (2 isolates), kluyveromyces marxianus (3 isolates) and rhodotorula mucilaginosa (1 isolate). surface hydrophobicity was measured by the microbial adhesion to solvents method. yeast cells and surfaces were ... | 2013 | 23837866 |
| determination of yeast diversity in ogi, mawè, gowé and tchoukoutou by using culture-dependent and -independent methods. | the maize based ogi and mawè and the sorghum based gowé and tchoukoutou are traditional, spontaneously fermented products widely consumed by the population of benin (west africa). yeast occurrence in the products, as sold on local markets at different locations, was studied using a combination of culture-dependent and independent methods. number of yeasts is varied from 3.75 log10 colony forming units (cfu)/g for ogi to 5.60 log10 cfu/g for tchoukoutou. isolated yeasts (236) were identified base ... | 2013 | 23727651 |
| biodiversity and probiotic potential of yeasts isolated from fura, a west african spontaneously fermented cereal. | fura is a spontaneously fermented pearl millet product consumed in west africa. the yeast species involved in the fermentation were identified by pheno- and genotypic methods to be candida krusei, kluyveromyces marxianus, candida tropicalis, candida rugosa, candida fabianii, candida norvegensis and trichosporon asahii. c. krusei and k. marxianus were found to be the dominant species. survival in ph 2.5 or in the presence of bile salts (0.3% (w/v) oxgall) and growth at 37°c were independently det ... | 2012 | 23072700 |
| yeasts from colombian kumis as source of peptides with angiotensin i converting enzyme (ace) inhibitory activity in milk. | this study investigated the possibility of using yeast strains in fermented milks to obtain products with high angiotensin i-converting enzyme (ace) inhibitory activity and low bitter taste. ninety-three yeast strains isolated from colombian kumis in different geographic regions were molecularly identified, and their milk fermentation performances were determined. molecular identification evidenced that galactomyces geotrichum, pichia kudriavzevii, clavispora lusitaniae and candida tropicalis, w ... | 2012 | 22938834 |
| isolation and characterisation of lactic acid bacteria from donkey milk. | during the last years the interest in donkey milk has increased significantly mainly because of its compelling functional elements. even if the composition and nutritional properties of donkey milk are known, its microbiota is less studied. this research communication aimed to provide a comprehensive characterisation of the lactic acid bacteria in raw donkey milk. rapd-pcr assay combined with 16s rdna sequencing analysis were used to describe the microbial diversity of several donkey farms in th ... | 2016 | 27600975 |
| genetic diversity of medically important and emerging candida species causing invasive infection. | genetic variation in the ribosomal dna (rdna) internal transcribed spacer (its) region has been studied among fungi. however, the numbers of its sequence polymorphisms in the various candida species and their associations with sources of invasive fungal infections remain poorly investigated. here, we characterized the intraspecific and interspecific its diversity of candida spp. strains collected from patients with bloodstream or oroesophageal candidiasis. | 2015 | 25887032 |
| yeasts from autochthonal cheese starters: technological and functional properties. | the aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties. | 2013 | 23600736 |
| protective effect of a mixture of kefir-isolated lactic acid bacteria and yeasts in a hamster model of clostridium difficile infection. | the objective of this work was to test the protective effect of a mixture (mm) constituted by kefir-isolated microorganisms (lactobacillus plantarum, lactobacillus kefir, lc. lactis, kluyveromyces marxianus and saccharomyces cerevisiae) in a hamster model of infection with clostridium difficile, an anaerobic gram-positive bacterium that causes diarrhoea. placebo or mm was administered ad libitum in drinking water from day 0 to the end of treatment. hamsters received orally 200 μg of clyndamicin ... | 2013 | 23542116 |
| kefir-isolated lactococcus lactis subsp. lactis inhibits the cytotoxic effect of clostridium difficile in vitro. | kefir is a dairy product obtained by fermentation of milk with a complex microbial population and several health-promoting properties have been attributed to its consumption. in this work, we tested the ability of different kefir-isolated bacterial and yeast strains (lactobacillus kefir, lb. plantarum, lactococcus lactis subps. lactis, saccharomyces cerevisiae and kluyveromyces marxianus) or a mixture of them (mm) to antagonise the cytopathic effect of toxins from clostridium difficile (tcda and ... | 2013 | 23217732 |
| characteristics of microbial biofilm on wooden vats ('gerles') in pdo salers cheese. | the purpose of this study was to characterize microbial biofilms from 'gerles' (wooden vats for making pdo salers cheese) and identify their role in milk inoculation and in preventing pathogen development. gerles from ten farms producing pdo salers cheese were subjected to microbial analysis during at least 4 periods spread over two years. they were distinguished by their levels of lactobacillus (between 4.50 and 6.01 log cfu/cm(2)), gram negative bacteria (between 1.45 and 4.56 log cfu/cm(2)), ... | 2012 | 22483401 |
| polymicrobial chronic endophthalmitis diagnosed by culture and molecular technique. | accurate etiological diagnosis is the key to prevention of ocular morbidity in endophthalmitis cases. a 66 year old male was suffering from chronic endophthalmitis post-cataract surgery. polymerase chain reaction examination on anterior chamber fluid was positive for propionibacterium acnes but negative for the panfungal genome. he was advised vitrectomy with intravitreal injections. polymerase chain reaction of vitreous aspirate was positive for p.acnes as well as panfungal genome. the vitreous ... | 2014 | 25008833 |
| taggiasca extra virgin olive oil colonization by yeasts during the extraction process. | the opalescent appearance of the newly produced olive oil is due to the presence of solid particles and microdrops of vegetation water in which the microorganisms from the olives' carposphere are trapped. present research has demonstrated that the microbiota of the fresh extracted olive oil, produced in the mills, is mainly composed of yeasts and to a lesser extent of molds. the close link between the composition of the microbiota of the olives' carposphere undergoing to processing, and that of ... | 2017 | 27889166 |
| coexistence of lactic acid bacteria and potential spoilage microbiota in a dairy processing environment. | microbial contamination in food processing plants can play a fundamental role in food quality and safety. in this study, the microbiota in a dairy plant was studied by both 16s rrna- and 26s rrna-based culture-independent high-throughput amplicon sequencing. environmental samples from surfaces and tools were studied along with the different types of cheese produced in the same plant. the microbiota of environmental swabs was very complex, including more than 200 operational taxonomic units with ... | 2015 | 26341209 |
| bioflavour production from tomato and pepper pomaces by kluyveromyces marxianus and debaryomyces hansenii. | bioflavours are called natural flavour and/or fragrance compounds which are produced using metabolic pathway of the microorganism and/or plant cells or their enzyme systems with bioengineering approaches. the aim of this study was to investigate bioflavour production from tomato and red pepper pomaces by kluyveromyces marxianus and debaryomyces hansenii. obtained specific growth rates of k. marxianus and d. hansenii in tomato pomace were 0.081/h and 0.177/h, respectively. the bioflavour profile ... | 2015 | 25614449 |
| construction of recombinant kluyveromyces marxianus ufv-3 to express dengue virus type 1 nonstructural protein 1 (ns1). | the yeast kluyveromyces marxianus is a convenient host for industrial synthesis of biomolecules. however, despite its potential, there are few studies reporting the expression of heterologous proteins using this yeast. here, we report expression of a dengue virus protein in k. marxianus for the first time. the dengue virus type 1 nonstructural protein 1 (ns1) was integrated into the k. marxianus ufv-3 genome at the lac4 locus using an adapted integrative vector designed for high-level expression ... | 2015 | 25085614 |
| susceptibility of species within the sporothrix schenckii complex to a panel of killer yeasts. | the sporothrix schenckii complex is the etiologic agent of sporotrichosis, a subacute or chronic mycosis which can affect humans and animals. killer yeasts have been used in the medical field for development of novel antimycotics and biotyping of pathogenic fungi. the action of 18 killer yeasts on the growth of 88 characterized s. schenckii, sporothrix globosa, sporothrix brasiliensis, and sporothrix mexicana clinical and environmental isolates was evaluated. killer studies were performed on pet ... | 2014 | 23686831 |
| impact of microbial cultures on proteolysis and release of bioactive peptides in fermented milk. | this study aimed at evaluating co-cultures of selected microorganisms for their proteolytic activity and capability to produce fermented milk enriched with ace-inhibitory (acei) peptides. selected yeasts (torulaspora delbruekii kl66a, galactomyces geotrichum kl20b, pichia kudriavzevii kl84a and kluyveromyces marxianus kl26a) and lactic acid bacteria strains (lactobacillus plantarum lat03, lb. plantarum klat01 and the not virulent enterococcus faecalis ke06) were screened as single cultures for t ... | 2014 | 24929726 |
| encapsulated whey-native yeast kluyveromyces marxianus as a feed additive for animal production. | whey is the main byproduct of the cheese industry. while the composition is variable, it retains up to 55% of milk nutrients. the beneficial features of whey indicates a promising source of new potentially probiotic strains for the development of food additives destined for animal production. the aim of this study was to identify kluyveromyces spp. isolated from whey, to study some probiotic properties and to select the best strain to be encapsulated using derivatised chitosan. kluyveromyces mar ... | 2017 | 28277172 |
| progress in the microbial production of s-adenosyl-l-methionine. | s-adenosyl-l-methionine (sam), which exists in all living organisms, serves as an activated group donor in a range of metabolic reactions, including trans-methylation, trans-sulfuration and trans-propylamine. compared with its chemical synthesis and enzyme catalysis production, the microbial production of sam is feasible for industrial applications. the current clinical demand for sam is constantly increasing. therefore, vast interest exists in engineering the sam metabolism in cells for increas ... | 2016 | 27465853 |
| molecular cloning and characterization of two ygl039w genes encoding broad specificity nadph-dependent aldehyde reductases from kluyveromyces marxianus strain dmb1. | two genes from kluyveromyces marxianus strain dmb1, ygl039w1 and ygl039w2, encode putative uncharacterized oxidoreductases that respectively share 42 and 44% identity with the saccharomyces cerevisiae s288c nadph-dependent methylglyoxal reductase (ec 1.1.1.283). to determine the enzymatic characteristics of their products, the two genes were expressed in recombinant escherichia coli cells, after which the ygl039w1 and ygl039w2 proteins were purified to homogeneity. in the presence of nadph, both ... | 2015 | 26223585 |
| characterization of the kluyveromyces marxianus strain dmb1 ygl157w gene product as a broad specificity nadph-dependent aldehyde reductase. | the open reading frame ygl157w in the genome of the yeast kluyveromyces marxianus strain dmb1 encodes a putative uncharacterized oxidoreductase. however, this protein shows 46% identity with the saccharomyces cerevisiae s288c nadph-dependent methylglyoxal reductase, which exhibits broad substrate specificity for aldehydes. in the present study, the ygl157w gene product (kmgre2) was purified to homogeneity from overexpressing escherichia coli cells and found to be a monomer. the enzyme was strict ... | 2015 | 25852994 |
| alcohol dehydrogenases from kluyveromyces marxianus: heterologous expression in escherichia coli and biochemical characterization. | kluyveromyces marxianus has recently become a species of interest for ethanol production since it can produce ethanol at high temperature and on a wide variety of substrates. however, the reason why this yeast can produce ethanol at high temperature is largely unknown. | 2014 | 24885162 |
| non-homologous end joining-mediated functional marker selection for dna cloning in the yeast kluyveromyces marxianus. | the cloning of dna fragments into vectors or host genomes has traditionally been performed using escherichia coli with restriction enzymes and dna ligase or homologous recombination-based reactions. we report here a novel dna cloning method that does not require dna end processing or homologous recombination, but that ensures highly accurate cloning. the method exploits the efficient non-homologous end-joining (nhej) activity of the yeast kluyveromyces marxianus and consists of a novel functiona ... | 2014 | 24307396 |
| a comparative study on galactoologosaccharide synthesis by selected beta-galactosidase preparations. | the transgalactosilating ability of beta-galactosidase preparations obtained from kluyveromyces fragilis, penicillium canescens, and escherichia coli was investigated. in samples of hydrolysates of 20% lactose solutions without or with a 2% addition of glucose, the galactose or fructose obtained with the use of the investigated preparations and the content of saccharides was determined with the hplc method. under experimental conditions, the highest quantity (56.0-64.1%) of galactooligosaccharid ... | 2003 | 24757791 |
| production of bioactive protein hydrolysate using the yeasts isolated from soft chhurpi. | the aim of this work was to study the production of bioactive protein hydrolysates using yeasts isolated from chhurpi. for this, a total of 125 proteolytic yeasts were isolated and molecular identification was carried out by analysis of the restriction digestion pattern generated by digesting the pcr amplified internal transcribed spacer region and 5.8s rrna gene (its1-5.8s-its2) using three endonucleases (haeiii, cfoi and hinfi). the results obtained showed that different proteolytic yeasts wer ... | 2016 | 27494105 |
| production of folate in oat bran fermentation by yeasts isolated from barley and diverse foods. | the focus of the research was to identify yeasts from barley kernels in order to study their folate production capability while maintaining high viscosity caused by soluble fibres in oat bran fermentation. | 2014 | 24916316 |
| microbial flora and some chemical properties of ersho, a starter for teff (eragrostis tef) fermentation. | ersho is a clear, yellow liquid that accumulates on the surface of fermenting teff-flour batter and is collected to serve as an inoculum for the next fermentation. the ph of ersho samples was about 3.5 and titratable acidity ranged between 3.1% and 5.7%. the mean aerobic mesophilic counts from four households varied between 6.9×10(6) and 1.3×10(8) c.f.u./ml and the aerobic bacterial flora consisted of bacillus spp. mean yeast counts ranged between 5.2×10(5) and 1.8×10(6) c.f.u./ml and comprised, ... | 1994 | 24420890 |
| biosynthesis of 2-phenylethanol from glucose with genetically engineered kluyveromyces marxianus. | 2-phenylethanol (2-pe) is an aromatic alcohol with a rose scent, which is used in the cosmetics, fragrance and food industries. 2-pe is produced in a few yeast strains by ehrlich pathway. in this study, kluyveromyces marxianus was genetically engineered for overproduction of 2-pe from glucose. about 1.0g/l of 2-pe was produced by overexpressing phenylpyruvate decarboxylase (aro10) and alcohol dehydrogenase (adh2) genes of saccharomyces cerevisiae. a similar level of 2-pe was also produced from e ... | 2014 | 24910335 |
| anti-salmonella activity and probiotic trends of kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 isolated from a french cheese, tomme d'orchies. | kluyveromyces marxianus s-2-05 and kluyveromyces lactis s-3-05 were recently isolated from a traditional french cheese, tomme d'orchies, and characterized here for their advantages using a different application perspective. first, we established their anti-salmonella activity and downregulation of the virulence sopd gene of salmonella enterica subsp. enterica serovar typhimurium, mainly in the presence of k. marxianus s-2-05. in addition to their antagonism, these non-saccharomyces yeasts were a ... | 2017 | 28366839 |
| enhanced fermentative performance under stresses of multiple lignocellulose-derived inhibitors by overexpression of a typical 2-cys peroxiredoxin from kluyveromyces marxianus. | bioethanol from lignocellulosic materials is of great significance to the production of renewable fuels due to its wide sources. however, multiple inhibitors generated from pretreatments represent great challenges for its industrial-scale fermentation. despite the complex toxicity mechanisms, lignocellulose-derived inhibitors have been reported to be related to the levels of intracellular reactive oxygen species (ros), which makes oxidoreductase a potential target for the enhancement of the tole ... | 2017 | 28360937 |
| utilization of inulin-containing waste in industrial fermentations to produce biofuels and bio-based chemicals. | inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, jerusalem artichoke, jicama, onion, wild yam, and yacón. to utilize inulin as its carbo ... | 2017 | 28341907 |
| identification of hexose kinase genes in kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction. | in yeast, the hexose assimilation is started at hexose phosphorylation. however, in kluyveromyces marxianus, the hexokinase (hxk) and glucokinase (glk) genes were not identified by experiments. meanwhile, the glucose-free fructose product requires more cost-efficient method. in this study, the kmhxk1 and kmglk1 genes were functionally identified through gene disruption, over-expression and recombinant enzymes characterization. both glucose and fructose assimilation ability decreased significantl ... | 2017 | 28338054 |
| transforming sugars into fat-lipid biosynthesis using different sugars in yarrowia lipolytica. | in an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as yarrowia lipolytica to convert waste materials into biofuels. here, we constructed two y. lipolytica strains that displayed both increased lipid accumulation and a more efficient use of biomass-derived sugars, including glucose, fructose, galactose, and inulin. the first strain, y. lipolytica ylz150, was derived from the french wild-type strain w29. it had inhibite ... | 2017 | 28303649 |
| comparative analysis of oxidative stress during aging of kluyveromyces marxianus in synthetic and whey media. | during the aging of yeast culture, kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. aged stationary phase cells were found to contain more reactive oxygen species. additionally, the level of oxidant is counteracted by the antioxidant defense system of the cells. comparison of 3-day-old culture of k. marxianus with 45-day stationary phase culture represents an increased level of ros inside the cells. moreover, a dec ... | 2017 | 28281180 |
| electron transport chain in a thermotolerant yeast. | yeasts capable of growing and surviving at high temperatures are regarded as thermotolerant. for appropriate functioning of cellular processes and cell survival, the maintenance of an optimal redox state is critical of reducing and oxidizing species. we studied mitochondrial functions of the thermotolerant kluyveromyces marxianus slp1 and the mesophilic off1 yeasts, through the evaluation of its mitochondrial membrane potential (δψm), atpase activity, electron transport chain (etc) activities, a ... | 2017 | 28181110 |
| genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in saccharomyces cerevisiae ir-2 for high-temperature ethanol production. | the pentose phosphate pathway (ppp) plays an important role in the efficiency of xylose fermentation during cellulosic ethanol production. in simultaneous saccharification and co-fermentation (sscf), the optimal temperature for cellulase hydrolysis of lignocellulose is much higher than that of fermentation. successful use of sscf requires optimization of the expression of ppp genes at elevated temperatures. this study examined the combinatorial expression of ppp genes at high temperature. the re ... | 2017 | 28181081 |
| erratum for inokuma et al., complete genome sequence of kluyveromyces marxianus nbrc1777, a nonconventional thermotolerant yeast. | | 2017 | 28153883 |
| oxidative stress and antioxidant response in a thermotolerant yeast. | stress tolerance is a key attribute that must be considered when using yeast cells for industrial applications. high temperature is one factor that can cause stress in yeast. high environmental temperature in particular may exert a natural selection pressure to evolve yeasts into thermotolerant strains. in the present study, three yeasts (saccharomyces cerevisiae, mc4, and kluyveromyces marxianus, off1 and slp1) isolated from hot environments were exposed to increased temperatures and were then ... | 2017 | 28094115 |
| trichoderma sp. spores and kluyveromyces marxianus cells magnetic separation: immobilization on chitosan-coated magnetic nanoparticles. | in the present study, the interactions between chitosan-coated magnetic nanoparticles (c-mnp) and trichoderma sp. spores as well as kluyveromyces marxianus cells were studied. by means of plackett-burman design, it was demonstrated that factors which directly influenced on yeast cells immobilization and magnetic separation were: inoculum and c-mnp quantity, stirring speed, interaction time, and volume of medium, while in the case of fungal spores, the temperature also was disclosed as an influen ... | 2016 | 28032818 |
| cellulose biorefinery based on a combined catalytic and biotechnological approach for production of 5-hmf and ethanol. | in this study, a combination of catalytic and biotechnological processes was proposed for the first time for application in a cellulose biorefinery for the production of 5-hydroxymethylfurfural (5-hmf) and bioethanol. hydrolytic dehydration of the mechanically activated microcrystalline cellulose over a carbon-based mesoporous sibunt-4 catalyst resulted in moderate yields of glucose and 5-hmf (21.1-25.1 and 6.6-9.4 %). 5-hmf was extracted from the resulting mixture with isobutanol and subjected ... | 2017 | 27995758 |
| chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast kluyveromyces marxianus. | cell wall polysaccharides from filamentous fungi and yeasts have been reported as antioxidant and antiproliferative polymers. thus, we evaluated these activities from cell wall polysaccharides from kluyveromyces marxianus cct7735. by using a centrifugal filter, a 203kda α-d-mannan (kmm-5) was obtained. kmm-5 exhibited no effect on hela cells and a weak antiproliferative activity against hep-g2 cells. in addition, at higher concentrations, it presented a cytotoxicity to the normal cell line, 3t3. ... | 2017 | 27987836 |
| engineered kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose. | xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. in this study, the pyruvate decarboxylase gene (kmpdc1) and glycerol-3-phosphate dehydrogenase gene (kmgpd1) of kluyveromyces marxianus yzj051 were disrupted to prevent ethanol and glycerol accumulation. the deficient growth of pdc disruption was rescued by overexpressing mutant kmmth1-δt. then pentose phosphate pathway and x ... | 2017 | 27955868 |
| kluyveromyces marxianus and saccharomyces boulardii induce distinct levels of dendritic cell cytokine secretion and significantly different t cell responses in vitro. | interactions between members of the intestinal microbiota and the mucosal immune system can significantly impact human health, and in this context, fungi and food-related yeasts are known to influence intestinal inflammation through direct interactions with specialized immune cells in vivo. the aim of the present study was to characterize the immune modulating properties of the food-related yeast kluyveromyces marxianus in terms of adaptive immune responses indicating inflammation versus toleran ... | 2016 | 27898740 |
| co-expression of two heterologous lactate dehydrogenases genes in kluyveromyces marxianus for l-lactic acid production. | lactic acid (la) is a versatile compound used in the food, pharmaceutical, textile, leather, and chemical industries. biological production of la is possible by yeast strains expressing a bacterial gene encoding l-lactate dehydrogenase (ldh). kluyveromyces marxianus is an emerging non-conventional yeast with various phenotypes of industrial interest. however, it has not been extensively studied for la production. in this study, k. marxianus was engineered to express and co-express various hetero ... | 2017 | 27867078 |
| isolation, identification and characterization of yeasts from fermented goat milk of the yaghnob valley in tajikistan. | the geographically isolated region of the yaghnob valley, tajikistan, has allowed its inhabitants to maintain a unique culture and lifestyle. their fermented goat milk constitutes one of the staple foods for the yaghnob population, and is produced by backslopping, i.e., using the previous fermentation batch to inoculate the new one. this study addresses the yeast composition of the fermented milk, assessing genotypic, and phenotypic properties. the 52 isolates included in this study revealed sma ... | 2016 | 27857705 |
| immobilization of yeast inulinase on chitosan beads for the hydrolysis of inulin in a batch system. | an extracellular inulinase was partially purified by ethanol precipitation and gel exclusion chromatography from a cell free extract of kluyveromyces marxianus. partially purified inulinase exhibited 420 iu/mg specific activity and it was immobilized on chitosan beads. activity yield of immobilized inulinase was optimized with glutaraldehyde concentration (1-5%), glutaraldehyde treatment time (30-240min), enzyme coupling-time (2-16h) and enzyme loading (5-30 iu) as functions. under the optimized ... | 2017 | 27845224 |
| modeling of exo-inulinase biosynthesis by kluyveromyces marxianus in fed-batch mode: correlating production kinetics and metabolic heat fluxes. | a metabolic heat-based model was used for estimating the growth of kluyveromyces marxianus, and the modified luedeking-piret kinetic model was used for describing the inulinase production kinetics. for the first time, a relationship was developed to relate inulinase production kinetics directly to metabolic heat generated, which corroborated well with the experimental data (with r (2) values of above 0.9). it also demonstrated the predominantly growth-associated nature of the inulinase productio ... | 2017 | 27844140 |
| selection of error-less synthetic genes in yeast. | conventional gene synthesis is usually accompanied by sequence errors, which are often deletions derived from chemically synthesized oligonucleotides. such deletions lead to frame shifts and mostly result in premature translational terminations. therefore, in-frame fusion of a marker gene to the downstream of a synthetic gene is an effective strategy to select for frame-shift-free synthetic genes. functional expression of fused marker genes indicates that synthetic genes are translated without p ... | 2017 | 27671945 |
| batch and fed-batch simultaneous saccharification and fermentation of primary sludge from pulp and paper mills. | primary sludge from a portuguese pulp and paper mill, containing 60% of carbohydrates, and unbleached pulp (as reference material), with 93% of carbohydrates, were used to produce ethanol by simultaneous saccharification and fermentation (ssf). ssf was performed in batch or fed-batch conditions without the need of a pretreatment. cellic(®) ctec2 was the cellulolytic enzymatic complex used and saccharomyces cerevisiae (baker's yeast or atcc 26602 strain) or the thermotolerant yeast kluyveromyces ... | 2016 | 27611735 |
| a kluyveromyces marxianus 2-deoxyglucose-resistant mutant with enhanced activity of xylose utilization. | thermotolerant ethanologenic yeast kluyveromyces marxianus is capable of fermenting various sugars including xylose but glucose represses to hamper the utilization of other sugars. to acquire glucose repression-defective strains, 33 isolates as 2-deoxyglucose (2-dog)-resistant mutants were acquired from about 100 colonies grown on plates containing 2-dog, which were derived from an efficient strain dmku 3-1042. according to the characteristics of sugar consumption abilities and cell growth and e ... | 2015 | 27611676 |
| draft genome sequence of the probiotic yeast kluyveromyces marxianus fragilis b0399. | here, we report the draft genome sequence of kluyveromyces marxianus fragilis b0399, the first yeast approved as a probiotic for human consumption not belonging to the genus saccharomyces the genome is composed of 8 chromosomes, with a total size of 11.44 mb, including mitochondrial dna. | 2016 | 27587830 |
| yeast derived from lignocellulosic biomass as a sustainable feed resource for use in aquaculture. | the global expansion in aquaculture production implies an emerging need of suitable and sustainable protein sources. currently, the fish feed industry is dependent on high-quality protein sources of marine and plant origin. yeast derived from processing of low-value and non-food lignocellulosic biomass is a potential sustainable source of protein in fish diets. following enzymatic hydrolysis, the hexose and pentose sugars of lignocellulosic substrates and supplementary nutrients can be converted ... | 2017 | 27558451 |
| high throughput, colorimetric screening of microbial ester biosynthesis reveals high ethyl acetate production from kluyveromyces marxianus on c5, c6, and c12 carbon sources. | advances in genome and metabolic pathway engineering have enabled large combinatorial libraries of mutant microbial hosts for chemical biosynthesis. despite these advances, strain development is often limited by the lack of high throughput functional assays for effective library screening. recent synthetic biology efforts have engineered microbes that synthesize acetyl and acyl esters and many yeasts naturally produce esters to significant titers. short and medium chain volatile esters have valu ... | 2016 | 27528369 |
| volatile metabolites produced from agro-industrial wastes by na-alginate entrapped kluyveromyces marxianus. | the aim of this study was to evaluate the effects of alginate entrapment on fermentation metabolites of kluyveromyces marxianus grown in agrowastes that served as the liquid culture media. k. marxianus cells entrapped in na-alginate were prepared using the traditional liquid-droplet-forming method. whey and pomaces from processed tomatoes, peppers, and grapes were used as the culture media. the changes in the concentrations of sugar, alcohol, organic acids, and flavor compounds were analyzed usi ... | 2017 | 27515464 |
| acquisition of the yeast kluyveromyces marxianus from unpasteurised milk by a kefir grain enhances kefir quality. | kefir is a fermented milk beverage consumed for nutritional and health tonic benefits in many parts of the world. it is produced by the fermentation of milk with a consortium of bacteria and yeast embedded within a polysaccharide matrix. this consortium is not well defined and can vary substantially between kefir grains. there are little data on the microbial stability of kefir grains, nor on interactions between microbes in the grain and in the milk. to study this, a grain was split, with one h ... | 2016 | 27369085 |
| differential rna-seq, multi-network analysis and metabolic regulation analysis of kluyveromyces marxianus reveals a compartmentalised response to xylose. | we investigated the transcriptomic response of a new strain of the yeast kluyveromyces marxianus, in glucose and xylose media using rna-seq. the data were explored in a number of innovative ways using a variety of networks types, pathway maps, enrichment statistics, reporter metabolites and a flux simulation model, revealing different aspects of the genome-scale response in an integrative systems biology manner. the importance of the subcellular localisation in the transcriptomic response is emp ... | 2016 | 27315089 |
| multilocus analysis reveals large genetic diversity in kluyveromyces marxianus strains isolated from parmigiano reggiano and pecorino di farindola cheeses. | in the present study, we have analysed the genetic diversity in kluyveromyces marxianus isolated from parmigiano reggiano and pecorino di farindola cheesemaking environment. molecular typing methods inter-rtl fingerprint and mtdna rflps, as well as, sequence diversity and heterozygosity in the intergenic region between kmssb1 and kmrio2 genes and analysis of the mating locus were applied to 54 k. marxianus strains. inter-rtl fingerprint revealed a large degree of genetic heterogeneity and cluste ... | 2016 | 27294555 |
| isolation, identification and oenological characterization of non-saccharomyces yeasts in a mediterranean island. | we isolated, identified and characterized yeast strains from grapes, and their fermented musts, sampled in the small island of linosa, where there are no wineries and therefore the possibility of territory contamination by industrial strains is minimal. by traditional culture-dependent methods, we isolated 3805 colonies, distinguished by molecular methods in 17 different species. five hundred and forty-four isolates were analysed for the main oenological characteristics such as fermentative vigo ... | 2016 | 27261362 |
| kluyveromyces marxianus as a host for heterologous protein synthesis. | the preferentially respiring and thermotolerant yeast kluyveromyces marxianus is an emerging host for heterologous protein synthesis, surpassing the traditional preferentially fermenting yeast saccharomyces cerevisiae in some important aspects: k . marxianus can grow at temperatures 10 °c higher than s. cerevisiae, which may result in decreased costs for cooling bioreactors and reduced contamination risk; has ability to metabolize a wider variety of sugars, such as lactose and xylose; is the fas ... | 2016 | 27260286 |
| simultaneous fermentation of glucose and xylose at elevated temperatures co-produces ethanol and xylitol through overexpression of a xylose-specific transporter in engineered kluyveromyces marxianus. | engineered kluyveromyces marxianus strains were constructed through over-expression of various transporters for simultaneous co-fermentation of glucose and xylose. the glucose was converted into ethanol, whereas xylose was converted into xylitol which has higher value than ethanol. over-expressing xylose-specific transporter scgal2-n376f mutant enabled yeast to co-ferment glucose and xylose and the co-fermentation ability was obviously improved through increasing scgal2-n376f expression. the pro ... | 2016 | 27240239 |
| enhanced exo-inulinase activity and stability by fusion of an inulin-binding module. | in this study, an inulin-binding module from bacillus macerans was successfully fused to an exo-inulinase from kluyveromyces marxianus, creating a hybrid functional enzyme. the recombinant exo-inulinase (rinu), the hybrid enzyme (rinuibm), and the recombinant inulin-binding module (ribm) were, respectively, heterologously expressed and biochemically characterized. it was found that both the inulinase activity and the catalytic efficiency (k cat/k m(app)) of the rinuibm were considerably higher t ... | 2016 | 27164865 |
| growth, ethanol production, and inulinase activity on various inulin substrates by mutant kluyveromyces marxianus strains nrrl y-50798 and nrrl y-50799. | economically important plants contain large amounts of inulin. disposal of waste resulting from their processing presents environmental issues. finding microorganisms capable of converting inulin waste to biofuel and valuable co-products at the processing site would have significant economic and environmental impact. we evaluated the ability of two mutant strains of kluyveromyces marxianus (km7 and km8) to utilize inulin for ethanol production. in glucose medium, both strains consumed all glucos ... | 2016 | 27130462 |
| immobilized kluyveromyces marxianus cells in carboxymethyl cellulose for production of ethanol from cheese whey: experimental and kinetic studies. | cheese whey fermentation to ethanol using immobilized kluyveromyces marxianus cells was investigated in batch and continuous operation. in batch fermentation, the yeast cells were immobilized in carboxymethyl cellulose (cmc) polymer and also synthesized graft copolymer of cmc with n-vinyl-2-pyrrolidone, denoted as cmc-g-pvp, and the efficiency of the two developed cell entrapped beads for lactose fermentation to ethanol was examined. the yeast cells immobilized in cmc-g-pvp performed slightly be ... | 2016 | 27126500 |
| a new method to determine optimum temperature and activation energies for enzymatic reactions. | a new method for determination of the optimum temperature and activation energies based on an idea of the average rate of enzymatic reaction has been developed. a mathematical model describing the effect of temperature on a dimensionless activity for enzyme deactivation following the first-order kinetics has been derived. the necessary condition for existence of the function extreme of the optimal temperature has been applied in the model. the developed method has been verified using the experim ... | 2016 | 27066801 |
| utilization of cheese whey using synergistic immobilization of β-galactosidase and saccharomyces cerevisiae cells in dual matrices. | whey is a byproduct of the dairy industry, which has prospects of using as a source for production of various valuable compounds. the lactose present in whey is considered as an environmental pollutant and its utilization for enzyme and fuel production, may be effective for whey bioremediation. the dairy yeast kluyveromyces marxianus have the ability to utilize lactose sharply as the major carbon source for the production of the enzyme. five strains were tested for the production of the β-galact ... | 2016 | 27059625 |