| antioxidant pathways are up-regulated during biological nitrogen fixation to prevent ros-induced nitrogenase inhibition in gluconacetobacter diazotrophicus. | gluconacetobacter diazotrophicus, an endophyte isolated from sugarcane, is a strict aerobe that fixates n(2). this process is catalyzed by nitrogenase and requires copious amounts of atp. nitrogenase activity is extremely sensitive to inhibition by oxygen and reactive oxygen species (ros). however, the elevated oxidative metabolic rates required to sustain biological nitrogen fixation (bnf) may favor an increased production of ros. here, we explored this paradox and observed that ros levels are, ... | 2010 | 20697694 |
| nutritional value of sugarcane silage enriched with corn grain, urea, and minerals as feed supplement on growth performance of beef steers grazing stargrass. | the objectives were to evaluate the silage quality of sugarcane silage enriched (as fed) with corn grain ground (10%), urea (1.5%), and mineral premix (0.5%) and its effects as a feed supplement on growth performance of beef steers grazing stargrass. firstly, in micro-silages, whole sugarcane enriched with corn grain, urea, and minerals (wsce) were ensilaged by 0, 20, 40, and 60 days. crude protein (cp) and lactic acid (la) increased linearly (p < 0.05) and true protein decreased linearly (p < 0 ... | 2011 | 20711753 |
| bacterially expressed dsrna protects maize against scmv infection. | rna interference (rnai) is a sequence-specific, posttranscriptional gene silencing (ptgs) process in plants that is mediated by dsrna homologous to the silenced gene(s). in this study, we report an efficient method to produce dsrna using a bacterial expression system. two fragments of the sugarcane mosaic virus (scmv) cp (coat protein) gene were amplified by rt-pcr, and cloned into the inverted-repeat cloning vector puccrnai. the two recombinant plasmids were transformed individually into e. col ... | 2010 | 20734050 |
| fermentation of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing. | this work aims to evaluate the fermentability of cellulosic hydrolysates obtained by enzymatic saccharification of sugarcane bagasse pretreated by hydrothermal processing using candida guilliermondii fti 20037 yeast. the inoculum was obtained from yeast culture in a medium containing glucose as a carbon source supplemented with rice bran extract, cacl(2).2h(2)o and (nh(4))(2)so(4) in 50 ml erlenmeyer flasks, containing 20 ml of medium, initial 5.5 ph under agitation of an orbital shaker (200 rpm ... | 2010 | 20740373 |
| production of cellulases and xylanases under catabolic repression conditions from mutant pr-22 of cellulomonas flavigena. | derepressed mutant pr-22 was obtained by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) mutagenic treatment of cellulomonas flavigena pn-120. this mutant improved its xylanolytic activity from 26.9 to 40 u mg(-1) and cellulolytic activity from 1.9 to 4 u mg(-1); this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. either glucose or cellobiose was added to cultures of c. flavigena pn-120 and mutant pr-22 induced with sugarcan ... | 2011 | 20803244 |
| kinetics of lime pretreatment of sugarcane bagasse to enhance enzymatic hydrolysis. | the objective of this work was to determine the optimum conditions of sugarcane bagasse pretreatment with lime to increase the enzymatic hydrolysis of the polysaccharide component and to study the delignification kinetics. the first stage was an evaluation of the influence of temperature, reaction time, and lime concentration in the pretreatment performance measured as glucose release after hydrolysis using a 2(3) central composite design and response surface methodology. the maximum glucose yie ... | 2011 | 20803263 |
| a new glycosidic flavonoid from jwarhar mahakashay (antipyretic) ayurvedic preparation. | the aqueous extract of jwarhar mahakashay ayurvedic preparation (from the roots of hemidesmus indicus r. br., rubia cordifolia l., cissampelos pareira l.; fruits of terminalia chebula retz., emblica officinalis gaertn., terminalia bellirica roxb., vitis vinifera l., grewia asiatica l., salvadora persica l. and granules of saccharum officinarum l.) has been used as a traditional antipyretic. experimental studies confirmed its antipyretic-analgesic effect with very low ulcerogenicity and toxicity. ... | 2010 | 20814525 |
| pretreatment of synthetic dairy wastewater using the sophorolipid-producing yeast candida bombicola. | the presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. in the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-producing yeast candida bombicola in a laboratory-scale bioreactor under batch, fed-batch, and continuous modes of operation. to support the yeast growth, the wastewater was suppleme ... | 2011 | 20821070 |
| heat shock modulates phosphorylation status and activity of nucleoside diphosphate kinase in cultured sugarcane cells. | nucleoside diphosphate kinase (ndpk) is involved in the regeneration of nucleoside triphosphates (ntps) through its phosphotransferase activity via an autophosphorylating histidine residue. additionally, autophosphorylation of serine and/or threonine residues is documented for ndpks from various organisms. however, the metabolic significance of serine/threonine phosphorylation has not been well characterized. in this study we report the cloning and characterization of ndpki from cultured sugarca ... | 2010 | 20821213 |
| bioconversion of novel substrate saccharum spontaneum, a weedy material, into ethanol by pichia stipitis ncim3498. | rising fuel prices and environmental issues have paved the way for the exploration of cellulosic ethanol. however, challenges involving substrate hydrolysis and cost-effectiveness still limit the efficient bioconversion and utilization of cellulosic ethanol. we aimed to evaluate a cheaper and abundantly available wild sugarcane variety, saccharum spontaneum, as the raw substrate for bioconversion of ethanol by pichia stipitis ncim3498. three different strategies for substrate hydrolysis using ac ... | 2011 | 20822899 |
| improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents. | inhibitory lignocellulose hydrolysates were treated with the reducing agents dithionite and sulfite to achieve improved fermentability. addition of these reducing agents (in the concentration range 5.0-17.5 mm) to enzymatic hydrolysates of spruce wood or sugarcane bagasse improved processes based on both shf (simultaneous hydrolysis and fermentation) and ssf (simultaneous saccharification and fermentation). the approach was exemplified in ethanolic fermentations with saccharomyces cerevisiae and ... | 2011 | 20822900 |
| isotachophoresis-based sample preparation of cellulases in sugarcane juice using bovine serum albumin as a model protein. | the foremost requirement of quantification of cellulases expressed in genetically modified sugarcane is an efficient sample clean-up. this work investigates the feasibility of isotachophoresis for this purpose. an electrolyte system comprising a leading electrolyte of 10mm formic acid at ph 9.0 and a terminating electrolyte of 10mm β-alanine was devised and used to perform isotachophoresis of cellulases. the use of a simple front cutting method removed a majority of interfering species in the ju ... | 2010 | 20833399 |
| sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection. | the aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. all experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in brazil for cattle feed. the process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. these parameters were applied to model the power required for an impeller to operate a ... | 2010 | 20844924 |
| biological detoxification of different hemicellulosic hydrolysates using issatchenkia occidentalis cctcc m 206097 yeast. | this work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using issatchenkia occidentalis cctcc m 206097 yeast. tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentrati ... | 2011 | 20844925 |
| structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae). | the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ... | 2011 | 20849253 |
| comparative evaluations of cellulosic raw materials for second generation bioethanol production. | to evaluate sugar recoveries and fermentabilities of eight lignocellulosic raw materials following mild acid pretreatment and enzyme hydrolysis using a recombinant strain of zymomonas mobilis. | 2010 | 20849394 |
| categorizing sugarcane cultivar resistance to the sugarcane aphid and yellow sugarcane aphid (hemiptera: aphididae). | sugarcane (saccharum spp.) in louisiana is colonized by two aphid species, the sugarcane aphid, melanaphis sacchari (zehntner), and the yellow sugarcane aphid, sipha flava (forbes) (hemiptera: aphididae). the main problem associated with m. sacchari is transmission of sugarcane yellow leaf virus, a casual agent of yellow leaf disease whose absence has been added to certification standards for micropropagated sugarcane in louisiana. greenhouse studies were conducted to categorize dominant commerc ... | 2010 | 20857758 |
| succinic acid production from sugarcane bagasse hemicellulose hydrolysate by actinobacillus succinogenes. | succinic acid, a four-carbon diacid, has been the focus of many research projects aimed at developing more economically viable methods of fermenting sugar-containing natural materials. succinic acid fermentation processes also consume co(2), thereby potentially contributing to reductions in co(2) emissions. succinic acid could also become a commodity used as an intermediate in the chemical synthesis and manufacture of synthetic resins and biodegradable polymers. much attention has been given rec ... | 2010 | 20882312 |
| seroprevalence of toxoplasmosis, leptospirosis and brucellosis in sugarcane field-workers in trinidad and tobago. | sugarcane field-workers, like rice field-workers, livestock farmers and abattoir workers are known to be occupationally exposed to zoonotic agents. the study determined the seroprevalence of immunoglobulins to toxoplasma gondii (igm), leptospira spp (igm) and brucella abortus (igg) in sugarcane field-workers across weighing stations in the island of trinidad. in addition, the association of risk factors to infections by the three zoonoses was investigated. blood samples were collected from conse ... | 2010 | 20931907 |
| genomic and evolutionary features of the spi-1 type iii secretion system that is present in xanthomonas albilineans but is not essential for xylem colonization and symptom development of sugarcane leaf scald. | xanthomonas albilineans is the causal agent of sugarcane leaf scald. interestingly, this bacterium, which is not known to be insect or animal associated, possesses a type iii secretion system (t3ss) belonging to the injectisome family salmonella pathogenicity island 1 (spi-1). the t3ss spi-1 of x. albilineans shares only low similarity with other available t3ss spi-1 sequences. screening of a collection of 128 plant-pathogenic bacteria revealed that this t3ss spi-1 is present in only two species ... | 2011 | 20955079 |
| pectin lyase production by recombinant penicillium griseoroseum strain 105. | recombinant penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (pl) under the transcriptional control of the strong gpda promoter of aspergillus nidulans. our aim was to evaluate pl production by recombinant p. griseoroseum strain 105 in submerged fermentation system bioreactors biofloiii and biofloiv using 2 or 10 l working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. pl ... | 2010 | 20962906 |
| unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator. | the potential of using vector-free minimal gene cassettes (mgcs) with a double terminator for the enhancement and stabilization of transgene expression was tested in sugarcane biolistic transformation. the mgc system used consisted of the enhanced yellow fluorescent protein (eyfp) reporter gene driven by the maize ubiquitin-1 (ubi) promoter and a single or double terminator from nopaline synthase (tnos) or/and cauliflower mosaic virus 35s (35st). transient eyfp expression from tnos or 35st singl ... | 2010 | 20967448 |
| [fermentation of two insoluble dietary fibers by colonic microflora in vitro]. | to investigate the rule of insoluble dietary fiber fermentation in intestinal tract. | 2010 | 21033443 |
| transgenic sugarcane plants expressing high levels of modified cry1ac provide effective control against stem borers in field trials. | to improve transgene expression level, we synthesized a truncated insecticidal gene m-cry1ac by increasing its gc content from 37.4 to 54.8%, based on the codon usage pattern of sugarcane genes, and transferred it into two sugarcane cultivars (roc16 and yt79-177) by microprojectile bombardment. the integration sites and expression pattern of the transgene were determined, respectively, by southern, northern and western blot analyses. the transgenic sugarcane lines produced up to 50 ng cry1ac pro ... | 2010 | 21046242 |
| production of fibrolytic enzymes by aspergillus japonicus c03 using agro-industrial residues with potential application as additives in animal feed. | solid-state fermentation obtained from different and low-cost carbon sources was evaluated to endocellulases and endoxylanases production by aspergillus japonicus c03. regarding the enzymatic production the highest levels were observed at 30 °c, using soy bran added to crushed corncob or wheat bran added to sugarcane bagasse, humidified with salt solutions, and incubated for 3 days (xylanase) or 6 days (cellulase) with 70% relative humidity. peptone improved the xylanase and cellulase activities ... | 2010 | 21046416 |
| microbial production of xylitol from d-xylose and sugarcane bagasse hemicellulose using newly isolated thermotolerant yeast debaryomyces hansenii. | a thermotolerant yeast capable of fermenting xylose to xylitol at 40°c was isolated and identified as a strain of debaryomyces hansenii by its sequencing. this paper reports the production of xylitol from d-xylose and sugarcane bagasse hemicellulose by free and ca-alginate immobilized cells of d. hansenii. the efficiency of free and immobilized cells were compared for xylitol production from d-xylose and hemicellulose in batch culture at 40°c. the maximum xylitol produced by free cells was 68.6 ... | 2010 | 21067918 |
| production of rhamnolipids in solid-state cultivation using a mixture of sugarcane bagasse and corn bran supplemented with glycerol and soybean oil. | rhamnolipid biosurfactants are attracting attention due to their low toxicity, high biodegradability, and good ecological acceptability. however, production in submerged culture is made difficult by severe foaming problems. solid-state cultivation (ssc) is a promising alternative production method. in the current work, we report the optimization of rhamnolipid production by pseudomonas aeruginosa ufpeda 614 on a solid substrate containing sugarcane bagasse and corn bran. the best rhamnolipid pro ... | 2010 | 21080163 |
| steam pretreatment and fermentation of the straw material "paja brava" using simultaneous saccharification and co-fermentation. | pretreatment, enzymatic hydrolysis and simultaneous saccharification and fermentation (ssf) of the south american straw material paja brava were investigated. suitable process conditions for an so₂-catalyzed steam pretreatment of the material were determined and assessed by enzymatic digestibility of obtained fiber slurries for 72 h at a water insoluble solids (wis) content of 2%. the best pretreatment conditions obtained (200 °c, 5 min holding time and 2.5% so₂) gave an overall glucose yield fo ... | 2010 | 21081285 |
| bioethanol production from dedicated energy crops and residues in arkansas, usa. | globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. lignocellulosic biomass of four dedicated energy crops [giant reed (arundo donax l.), elephantgrass (pennisetum purpureum (schumach), miscanthus × giganteus (illinois clone), and (clone q42641) {hybrid of miscanthus sinensis anderss. and miscanthus sacchariflorus (maxim)}, hack. called giant miscanthus, and sugarcane clone us 84-1028 (saccharum l. spp. hybrid)] an ... | 2011 | 21086455 |
| development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process. | the present study verified an applicable technology of xylitol bioconversion as part of the integration of co-product generation within second-generation bioethanol processes. a newly isolated yeast strain, candida tropicalis jh030, was shown to have a capacity for xylitol production from hemicellulosic hydrolysate without detoxification. the yeast gives a promising xylitol yield of 0.71 g(p) g(s)(-1) from non-detoxified rice straw hydrolysate that had been prepared by the dilute acid pretreatme ... | 2010 | 21095119 |
| gluconacetobacter diazotrophicus levansucrase is involved in tolerance to nacl, sucrose and desiccation, and in biofilm formation. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane, which expresses levansucrase, a fructosyltransferase exoenzyme with sucrose hydrolytic and levan biosynthetic activities. as a result of their physical properties, the levan can provide protection against stress caused by abiotic or biotic factors and participate in the formation of biofilms. in this study, we investigated the construction and function of a levansucrase-defective mutant of g. diazotrophic ... | 2010 | 21103984 |
| developmental and photosynthetic regulation of δ-endotoxin reveals that engineered sugarcane conferring resistance to 'dead heart' contains no toxins in cane juice. | the phosphoenolpyruvate (pep) carboxylase is regulated at the levels of transcription and post-translation in c4 plants in light and abundantly accumulates in leaf mesophyll cells. we report here developmental and photosynthetic regulation of stably accumulated bacillus thuringiensis δ-endotoxin under the control of pep-c promoter in transgenic sugarcane. in young leaves of plants, the transprotein is accumulated to 39% of the levels in mature leaves (135 ng mg(-1)), and is induced with the cell ... | 2010 | 21104030 |
| the effect of biomass moisture content on bioethanol yields from steam pretreated switchgrass and sugarcane bagasse. | this study aimed to determine the effect of moisture content of three different feedstocks on overall ethanol yield. switchgrass and sugarcane bagasse from two sources were either soaked in water (∼80% moisture) or left dry (∼12% moisture), and half each of these were impregnated with 3%w/w so(2) and all were steam pretreated. the twelve resulting substrates were compared based on overall sugar recovery after pretreatment, cellulose conversion following enzymatic hydrolysis, and ethanol yield fo ... | 2010 | 21109425 |
| simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant escherichia coli strain mm160. | hexose and pentose sugars from phosphoric acid pretreated sugarcane bagasse were co-fermented to ethanol in a single vessel (sscf), eliminating process steps for solid-liquid separation and sugar cleanup. an initial liquefaction step (l) with cellulase was included to improve mixing and saccharification (l+sscf), analogous to a corn ethanol process. fermentation was enabled by the development of a hydrolysate-resistant mutant of escherichia coli ly180, designated mm160. strain mm160 was more res ... | 2010 | 21111615 |
| a specific, robust, and automated method for routine at-line monitoring of the concentration of cellulases in genetically modified sugarcane plants. | bagasse is one of the waste crop materials highlighted as commercially viable for cellulosic bio-ethanol production via enzymatic conversion to release fermentable sugars. genetically modified sugarcane expressing cellobiohydrolases (cbh), endoglucanase (eg), and β-glucosidases (bg) provide a more cost-effective route to cellulose breakdown compared to culturing these enzymes in microbial tanks. hence, process monitoring of the concentration profile of these key cellulases in incoming batches of ... | 2010 | 21136205 |
| weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'biofuel'. | lignocellulosic materials are the most abundant renewable organic resources (~200 billion tons annually) on earth that are readily available for conversion to ethanol and other value-added products, but they have not yet been tapped for the commercial production of fuel ethanol. the lignocellulosic substrates include woody substrates such as hardwood (birch and aspen, etc.) and softwood (spruce and pine, etc.), agro residues (wheat straw, sugarcane bagasse, corn stover, etc.), dedicated energy c ... | 2010 | 21181146 |
| bioconversion of saccharum spontaneum (wild sugarcane) hemicellulosic hydrolysate into ethanol by mono and co-cultures of pichia stipitis ncim3498 and thermotolerant saccharomyces cerevisiae-vs(3). | the lignocellulosic biomass is a low-cost renewable resource for eco-benign liquid fuel 'ethanol'. to resolve the hydrolysis of mixed sugars in lignocellulosic substrate saccharum spontaneum, the microbial co-cultures of pichia stipitis ncim 3498 and thermotolerant saccharomyces cerevisiae-vs(3) were analyzed for efficient bioconversion of mixed sugars into ethanol. among the hydrolysis conditions, the acid hydrolysis released maximum sugars along with furans, phenolics and acetic acid. the acid ... | 2010 | 21185411 |
| quantitative proteomic analysis of the interaction between the endophytic plant-growth-promoting bacterium gluconacetobacter diazotrophicus and sugarcane. | gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium that colonizes sugarcane. in order to investigate molecular aspects of the g. diazotrophicus-sugarcane interaction, we performed a quantitative mass spectrometry-based proteomic analysis by (15)n metabolic labeling of bacteria, root samples, and co-cultures. overall, more than 400 proteins were analyzed and 78 were differentially expressed between the plant-bacterium interaction model and control cultures. a comparative analy ... | 2011 | 21190439 |
| identification of new candidate pathogenicity factors in the xylem-invading pathogen xanthomonas albilineans by transposon mutagenesis. | xanthomonas albilineans is a xylem-invading pathogen that produces the toxin albicidin that blocks chloroplast differentiation, resulting in disease symptoms of sugarcane leaf scald. in contrast to other xanthomonads, x. albilineans does not possess a hypersensitive response and pathogenicity type iii secretion system and does not produce xanthan gum. albicidin is the only previously known pathogenicity factor in x. albilineans, yet albicidin-deficient mutant strains are still able to efficientl ... | 2011 | 21190440 |
| genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan. | bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ... | 2010 | 21193815 |
| genomic evidence of intraspecific recombination in sugarcane mosaic virus. | the sugarcane mosaic virus (scmv) of the genus potyvirus, which primarily affects maize, sugarcane, sorghum, abaca, and grasses, occurs worldwide and causes significant economic loss. using the full genome sequences of scmv and several recombination detection methods, in this study we report that recombination is the major driving force in the evolution and emergence of several new variants of scmv. we reported eight highly significant (p < 0.001) recombination break points, majority of which ar ... | 2010 | 21193953 |
| the genome sequence of e. coli w (atcc 9637): comparative genome analysis and an improved genome-scale reconstruction of e. coli. | escherichia coli is a model prokaryote, an important pathogen, and a key organism for industrial biotechnology. e. coli w (atcc 9637), one of four strains designated as safe for laboratory purposes, has not been sequenced. e. coli w is a fast-growing strain and is the only safe strain that can utilize sucrose as a carbon source. lifecycle analysis has demonstrated that sucrose from sugarcane is a preferred carbon source for industrial bioprocesses. | 2011 | 21208457 |
| bioaccumulation of the synthetic dye basic violet 3 and heavy metals in single and binary systems by candida tropicalis grown in a sugarcane bagasse extract medium: modelling optimal conditions using response surface methodology (rsm) and inhibition kinetics. | single and binary effects of dye basic violet 3 and heavy metals, 'namely', pb(ii) and cd(ii), were investigated for their role in dye and heavy metal bioaccumulation by candida tropicalis that was grown in a sugarcane bagasse extract medium containing 8 g/l, 16 g/l or 24 g/l of sugar. the optimum ph was found to be 4.0 in the single system and 5.0 in the binary system. a central composite design was successfully used to analyse the experimental results. four numerical correlations that were fit ... | 2010 | 21215516 |
| biotechnology tools in agriculture: recent patents involving soybean, corn and sugarcane. | the technological opportunities opened up by biotechnology in agriculture are diverse, including plant breeding, the partial or total relief of pesticides chemicals usage, the improvement of soil fertility, the improvement of the quality attributes of various foods. specifically, various tricks of biotechnology can be used for higher seed yield, resistance to diseases and insects, better stems and roots, tolerance to drought and heat, and better agronomic quality. a number of recent works consid ... | 2011 | 21226663 |
| short distance movement of genomic negative strands in a host and nonhost for sugarcane mosaic virus (scmv). | in order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication, both positive and negative sugarcane mosaic virus (scmv) strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one mexican potyviral isolate (scmv-ver1). intermediary replication forms, such as the negative viral strand, seem to only move a short distance as surveyed by rt-pcr analysis and elisa in differen ... | 2011 | 21232133 |
| ammonium carboxylate production from sugarcane trash using long-term air-lime pretreatment followed by mixed-culture fermentation. | sugarcane trash (st) was converted to ammonium carboxylates using a novel bioprocessing strategy known as long-term air-lime pretreatment/mixed-culture fermentation. at mild conditions (50°c, 5 weeks, 1-atm air, and excess lime loading of 0.4 g ca(oh)(2)/(g dry biomass)), air-lime pretreatment of st had moderate delignification (64.4%) with little loss in polysaccharides. without employing detoxification, sterility, expensive nutrients, or costly enzymes, the feedstock (80% treated st/20% chicke ... | 2010 | 21232948 |
| digestive physiology and characterization of digestive cathepsin l-like proteinase from the sugarcane weevil sphenophorus levis. | sugarcane is an important crop that has recently become subject to attacks from the weevil sphenophorus levis, which is not efficiently controlled with chemical insecticides. this demands the development of new control devices for which digestive physiology data are needed. in the present study, ion-exchange chromatography of s. levis whole midgut homogenates, together with enzyme assays with natural and synthetic substrates and specific inhibitors, demonstrated that a cysteine proteinase is a m ... | 2011 | 21256130 |
| production of astaxanthin from cellulosic biomass sugars by mutants of the yeast phaffia rhodozyma. | astaxanthin is a potential high-value coproduct in an ethanol biorefinery. three mutant strains of the astaxanthin-producing yeast phaffia rhodozyma, which were derived from the parent strain atcc 24202 (ucd 67-210) and designated jtm166, jtm185, and ssm19, were tested for their capability of utilizing the major sugars that can be generated from cellulosic biomass, including glucose, xylose, and arabinose, for astaxanthin production. while all three strains were capable of metabolizing these sug ... | 2011 | 21274657 |
| use of soybean vinasses as a germinant medium for a geobacillus stearothermophilus atcc 7953 sterilization biological indicator. | a novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (bi). various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained bi. media performance was evaluated by viability and heat resistance (d(121 °c) value) according to regulatory standards. a medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) p ... | 2011 | 21336685 |
| [sugarcane biopolymer membrane: experimental evaluation in the middle ear]. | new developments on biomaterials are important in surgery. the behavior of a new membrane produced from sugarcane will be evaluated in the middle ear of rats. | 2011 | 21340188 |
| production and optimization of cellulase enzyme using aspergillus niger usm ai 1 and comparison with trichoderma reesei via solid state fermentation system. | novel design solid state bioreactor, fermsostat, had been evaluated in cellulase production studies using local isolate aspergillus niger usm ai 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. under optimised ssf conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30°c; aeration at 4 l/h · g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 u/g of filter paper activity (fpase) was obtained. at the same time, comparative studies of the enzyme ... | 2010 | 21350665 |
| effect of reduced sulfur compounds on the fermentation of phosphoric acid pretreated sugarcane bagasse by ethanologenic escherichia coli. | the addition of reduced sulfur compounds (thiosulfate, cysteine, sodium hydrosulfite, and sodium metabisulfite) increased growth and fermentation of dilute acid hydrolysate of sugarcane bagasse by ethanologenic escherichia coli (strains ly180, emfr9, and mm160). with sodium metabisulfite (0.5mm), toxicity was sufficiently reduced that slurries of pretreated biomass (10% dry weight including fiber and solubles) could be fermented by e. coli strain mm160 without solid-liquid separation or cleanup ... | 2011 | 21353535 |
| immunotherapeutic effects of some sugar cane (saccharum officinarum l.) extracts against coccidiosis in industrial broiler chickens. | present paper reports the effects of aqueous and ethanolic extracts of sugar cane (saccharum officinarum l.) juice and bagasse, respectively on protective immune responses in industrial broiler chickens against coccidiosis. immunotherapeutic efficacies of the extracts were measured by evaluating their effect on body weight gain, oocyst shedding, lesion score, anti-coccidial indices, per cent protection and elicited serum antibody responses against coccidiosis. results revealed a significantly lo ... | 2011 | 21354144 |
| d(-)-lactic acid production by leuconostoc mesenteroides b512 using different carbon and nitrogen sources. | sugar concentration from sugarcane juice and yeast autolysate increased lactic acid production more than the other agro-industrial substrates tested. the concentrations of these two components were further optimized using the plackett-burman design and response surface method. a second-order polynomial regression model estimated that a maximal lactic acid production of 66.11 g/l would be obtained when the optimal values of sugar and yeast autolysate were 116.9 and 44.25 g/l, respectively. to val ... | 2011 | 21360091 |
| anaerobic thermophilic fermentation for carboxylic acid production from in-storage air-lime-treated sugarcane bagasse. | wet storage and in situ lime pretreatment (50 °c, 1-atm air, 56 days, excess lime loading of 0.3 g ca(oh)(2)/g dry biomass) of sugarcane bagasse (4,000 g dry weight) was performed in a bench-scale pile pretreatment system. under thermophilic conditions (55 °c, nh(4)hco(3) buffer, methane inhibitors), air-lime-treated bagasse (80 wt.%) and chicken manure (20 wt.%) were anaerobically co-digested in 1-l rotary fermentors by a mixed culture of marine microorganisms (galveston, tx). during four-stage ... | 2011 | 21365471 |
| duplex-immunocapture-rt-pcr for detection and discrimination of two distinct potyviruses naturally infecting sugarcane (saccharum spp. hybrid). | a sensitive duplex-immunocapture-rt-pcr (d-ic-rt-pcr) technique was developed for detection and discrimination of taxonomically distinct sugarcane streak mosaic virus (scsmv) and sugarcane mosaic virus (scmv) that naturally infect sugarcane. d-ic-rt-pcr was performed using polyclonal antisera for capture of virions. oligo 5'-d(t)18(agc)-3' as a common reverse primer for both viruses and virus specific forward primers, 5'-aagtggttaaacgcctgtgg-3' and 5'-atgtc(ga)aagaa(ga)atgcgcttgc-3' were used fo ... | 2011 | 21365999 |
| improvement of ethanol production using saccharomyces cerevisiae by enhancement of biomass and nutrient supplementation. | optimization of ethanol production through addition of substratum and protein-lipid additives was studied. oilseed meal extract was used as protein lipid supplement, while rice husk was used as substratum. the effect of oil seed meal extract and rice husk was observed at varying concentration of medium sugar from 8% to 20%. of the three oil seed meal extracts used, viz. groundnut, safflower, and sunflower, safflower was found to be most efficient. the use of oilseed meal extract at 4% was found ... | 2011 | 21373793 |
| a rare autosomal recessive condition, congenital erythropoietic porphyria, found in the canefield rat rattus sordidus gould 1858. | congenital erythropoietic porphyria (cep) is a rare autosomal recessive condition that has been reported in humans and in some animals, in which uroporphyrin 1 is deposited in the bones, teeth and urine, resulting in pink coloration and fluorescence of the tissues and urine under long-wave ultraviolet (uv) light. we observed red teeth in nine of 450 canefield rats (rattus sordidus) captured in a small, isolated patch of sugarcane in tully, north queensland, australia. the skeletons of these anim ... | 2008 | 21396071 |
| enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of pichia kudriavzevii. | the thermotolerant yeast strain isolated from sugarcane juice through enrichment technique was identified as a strain of pichiakudriavzevii (issatchenkiaorientalis) through molecular characterization. the p. kudriavzevii cells adapted to galactose medium produced about 30% more ethanol from sugarcane juice than the non-adapted cells. the recycled cells could be used for four successive cycles without a significant drop in ethanol production. fermentation in a laboratory fermenter with galactose ... | 2011 | 21398115 |
| peroxisomal polyhydroxyalkanoate biosynthesis is a promising strategy for bioplastic production in high biomass crops. | polyhydroxyalkanoates (phas) are bacterial carbon storage polymers with diverse plastic-like properties. pha biosynthesis in transgenic plants is being developed as a way to reduce the cost and increase the sustainability of industrial pha production. the homopolymer polyhydroxybutyrate (phb) is the simplest form of these biodegradable polyesters. plant peroxisomes contain the substrate molecules and necessary reducing power for phb biosynthesis, but peroxisomal phb production has not been explo ... | 2011 | 21447054 |
| biodegradation of basic violet 3 by candida krusei isolated from textile wastewater. | basic violet 3 (bv) belongs to the most important group of synthetic colorants and is used extensively in textile industries. it is considered as xenobiotic compound which is recalcitrant to biodegradation. as candida krusei could not use bv as sole carbon source, experiments were conducted to study the effect of cosubstrates on decolorization of bv in semi synthetic medium using glucose, sucrose, lactose, maltose, yeast extract, peptone, urea and ammonium sulphate. maximum decolorization (74%) ... | 2011 | 21479732 |
| mutagenic activity of airborne particulate matter (pm(10)) in a sugarcane farming area (araraquara city, southeast brazil). | brazil contains 25% of the total land planted with sugarcane in the world and is thus one of the major producers. the annual burning of sugarcane fields prior to harvesting emits huge amounts of pyrogenic particles. biomass burning is an important primary and secondary source of aerosol particles. the presence of carbonaceous particles in the inhalable size range makes it important to study this fraction in view of the possible effects on human health and the climate. in this study, the mutageni ... | 2011 | 21481367 |
| biological control of red rot in sugarcane by native pyoluteorin-producing pseudomonas putida strain nh-50 under field conditions and its potential modes of action. | background: rhizobacteria have a good potential to suppress soilborne diseases, but their efficacy against sugarcane pests is rarely reported. bacterial strains isolated from the rhizosphere of sugarcane were evaluated for their potential to suppress red rot disease on two susceptible varieties, co-1148 and spf-234, under field conditions. the strains were also characterised for the production of secondary metabolites associated with their antagonistic activity. results: one out of four strains, ... | 2011 | 21495154 |
| production of sophorolipid glycolipid biosurfactants from sugarcane molasses using starmerella bombicola nbrc 10243. | biosurfactants (bs) are produced by a variety of microorganisms from renewable resources, and have unique properties compared to chemical surfactants. in order to attain efficient production of bs from low-cost materials, we focused our attention on the use of sugarcane molasses. fifteen yeast strains that are known as bs producers were examined for bs productivity from a culture medium consisting of only molasses and water. among the strains tested, only starmerella bombicola nbrc 10243 produce ... | 2011 | 21502725 |
| prevalence of plasmid mediated pesticide resistant bacterial assemblages in crop fields. | three crop fields namely paddy sugarcane and tomato exposed to bavistin [methyl (1h-benzimidazol-2-yl) carbomate], monocrotophos[dimethyl(e)-1-methyl-2-(methyl-carbamoyl) vinyl phosphate] and kinado plus [(ez)-2-chloro-3-dimethoxyphosphinoyloxy-x1, x1-diethylbut-2-enamide], respectively were chosen for the present investigation to know the bacterial population and degradation of pesticides. the chemical nature of the soil and water samples from the pesticide contaminated fields was analysed alon ... | 2010 | 21506482 |
| injection of air into the headspace improves fermentation of phosphoric acid pretreated sugarcane bagasse by escherichia coli mm170. | microaeration (injecting air into the headspace) improved the fermentation of hemicellulose hydrolysates obtained from the phosphoric acid pretreatment of sugarcane bagasse at 170°c for 10min. in addition, with 10% slurries of phosphoric acid pretreated bagasse (180°c, 10min), air injection into the headspace promoted xylose utilization and increased ethanol yields from 0.16 to 0.20g ethanol/g bagasse dry weight using a liquefaction plus simultaneous saccharification and co-fermentation process ... | 2011 | 21531547 |
| molecular analysis of the complete genomic sequences of four isolates of gooseberry vein banding associated virus. | the presence of gooseberry vein banding associated virus (gvbav), a badnavirus in the family caulimoviridae, is strongly correlated with gooseberry vein banding disease in ribes spp. in this study, full-length genomic sequences of four gvbav isolates from different hosts and geographic regions were determined to be 7649-7663 nucleotides. these isolates share identities of 96.4-97.3% for the complete genomic sequence, indicating low genetic diversity among them. the gvbav genome contains three op ... | 2011 | 21533750 |
| f(2) screening for resistance to pyramided bacillus thuringiensis maize in louisiana and mississippi populations of diatraea saccharalis (lepidoptera: crambidae). | background: in the mid-southern region of the united states, sugarcane borer, diatraea saccharalis (f.), is a major target pest of transgenic maize expressing bacillus thuringiensis (bt) proteins. novel transgenic maize technologies containing two or more pyramided bt genes for controlling lepidopteran pests have recently become commercially available. insect resistance management (irm) is an important issue in the sustainable use of bt crop technologies. the objective of this study was to deter ... | 2011 | 21538799 |
| broad distribution and phylogeny of anaerobic endophytes of cluster xiva clostridia in plant species including crops. | endophytic clostridia present on various plants as obligate anaerobes were surveyed by terminal restriction fragment length polymorphism (trflp) analysis specific to the clostridial 16s rrna gene. endophytic clostridia were detected in 10 plant types: sugarcane, cultivated rice, corn, tobacco, soybean, bermuda grass, tall fescue, and three mangrove species. phylogenetically, cluster xiva clostridia were detected more frequently than cluster i clostridia in aerial parts. isolation of clostridia f ... | 2008 | 21558691 |
| infection, multiplication and evaluation of the nitrogen-fixing ability of herbaspirillum sp. strain b501gfp1 in sugarcane stems inoculated by the vacuum infiltration method. | the present study was conducted with the aim of finding a suitable delivery method for introducing endophytic bacteria into new sugarcane stalks. stem pieces of cultivars nif8 and ni15 were inoculated with herbaspirillum sp. strain b501gfp1 by the vacuum infiltration method. the inoculated bacteria showed higher levels of nitrogenase activity in cultivar ni15 than nif8. the population density immediately after inoculation (10(7) cfu g [fresh weight](-1)) showed that strain b501gfp1 successfully ... | 2008 | 21558698 |
| impact of organic fertilizers with and without chemical fertilizers on soil chemical properties and the establishment of nitrogen-fixing bacteria in the rhizosphere. | effects of organic fertilizers with and without the application of chemical fertilizers for seven years as part of a wheat-pearl millet cropping sequence on soil chemical properties and the establishment of nitrogen fixing bacteria in the rhizosphere were examined. the application of farmyard manure, poultry manure, and sugarcane filter cake alone or in combination with chemical fertilizers improved the soil organic c, total n, p, and k status. larger populations of azotobacter chroococcum and r ... | 2008 | 21558724 |
| laboratory and field evaluation of metarhizium anisopliae var. anisopliae for controlling subterranean termites. | the efficacy of the metarhizium anisopliae strain arsef 6911 was determined in the laboratory and field against two sugarcane pests, microtermes obesi holmgren and odontotermes obesus rambur (termitidae: isoptera). the susceptibility of both termite species to different conidial suspensions (1 × 10(10), 1 × 10(8), 1 × 10(6) and 1 × 10(4) conidia/ml) was determined in laboratory. all conidial suspensions were able to induce mortality. termite mortality caused by the fungal suspensions was dose de ... | 2011 | 21584407 |
| global warming, plant paraquat resistance, and light signal transduction through nucleoside diphosphate kinase as a paradigm for increasing food supply. | light signal transduction was studied in extracts of mycelia of the fungus neurospora crassa, and the third internodes of dark-grown pisum sativum cv alaska. both processes increased the phosphorylation of nucleoside diphosphate kinase (ndpk). ndpk may function as a carrier of reduction equivalents, as it binds nadh, thereby providing electrons to transform singlet oxygen to superoxide by catalases (cat). as the c-termini of ndpk interact with cat which receive singlet oxygen, emitted from photo ... | 2011 | 21603975 |
| isolation of pseudozyma churashimaensis sp. nov., a novel ustilaginomycetous yeast species as a producer of glycolipid biosurfactants, mannosylerythritol lipids. | an ustilaginomycetous anamorphic yeast species isolated from the leaves of saccharum officinarum (sugarcane) in okinawa, japan, was identified as a novel pseudozyma species based on morphological and physiological aspects and molecular taxonomic analysis using the d1/d2 domains of the large subunit (26s) rrna gene and the internal transcribed spacer 1 (its1)-5.8s-its2 regions. the name pseudozyma churashimaensis sp. nov. was proposed for the novel species, with jcm 16988(t) as the type strain. i ... | 2011 | 21606002 |
| candida middelhoveniana sp. nov., a new yeast species found on the rhizoplane of organically cultivated sugarcane. | a novel yeast species within the metschnikowiaceae is described based on a strain from the sugarcane (saccharum sp.) rhizoplane of an organically managed farm in rio de janeiro, brazil. the d1/d2 domain of the large subunit ribosomal rna gene sequence analysis showed that the closest related species were candida tsuchiyae with 86.2% and candida thailandica with 86.7% of sequence identity. all three are anamorphs in the clavispora opuntiae clade. the name candida middelhoveniana sp. nov. is propo ... | 2011 | 21614495 |
| ethanol production by a new pentose-fermenting yeast strain, scheffersomyces stipitis ufmg-imh 43.2, isolated from the brazilian forest. | the ability of a recently isolated scheffersomyces stipitis strain (ufmg-imh 43.2) to produce ethanol from xylose was evaluated. for the assays, a hemicellulosic hydrolysate produced by dilute acid hydrolysis of sugarcane bagasse was used as the fermentation medium. initially, the necessity of adding nutrients (mgso(4) ·7h(2) o, yeast extract and/or urea) to this medium was verified, and the yeast extract supplementation favoured ethanol production by the yeast. then, in a second stage, assays u ... | 2011 | 21626536 |
| co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus for simultaneous biosynthesis of xylanase and laccase under solid-state fermentation. | co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 was evaluated for the production of xylanase-laccase mixture under solid-state fermentation (ssf) condition. growth compatibility between mutant p. oxalicum sau(e)-3.510 and white rot fungi (p. ostreatus mtcc 1804, trametes hirsuta mtcc 136 and pycnoporus sp. mtcc 137) was analyzed by growing them on potato dextrose agar plate. extracellular enzyme activities were determined spectrophotometrically. under ... | 2011 | 21642024 |
| optimization of fibrolytic enzyme production by aspergillus japonicus c03 with potential application in ruminant feed and their effects on tropical forages hydrolysis. | fibrolytic enzyme production by aspergillus japonicus c03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. a 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. tropical forages were hydrolyzed by a. japonicus c03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. optimal production to xylanase was obtained with soybean bran added ... | 2011 | 21647681 |
| responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae. | genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ... | 2011 | 21667271 |
| nutrient budgets (c, n and p) and trophic dynamics of a brazilian tropical estuary: barra das jangadas. | this paper focuses on the nutrient dynamics of a tropical estuary on the northeastern brazilian coast, studied using the loicz biogeochemical budgeting protocol. we describe the methodology and assumptions underlying this model. input data (monthly for rainfall, evaporation, river discharge, and concentrations of salt, phosphorus and nitrogen) were obtained during field campaigns in the barra das jangadas estuary (bje) over a 5 years period (1999 to 2003). mass balance results indicate large inp ... | 2011 | 21670871 |
| bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. | a gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated sc-n012(t), was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. the strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus bacillus. sequence analysis of the 16s rrna gene of sc-n012(t) revealed the closest match (98.9% pair wise similarity) with bacillus clausii dsm 8716(t). however, dna- ... | 2011 | 21671194 |
| differential gene expression in sugarcane in response to challenge by fungal pathogen ustilago scitaminea revealed by cdna-aflp. | differential gene expression in sugarcane during sugarcane-ustilago scitaminea interaction was conducted in a smut-resistant genotype. using cdna-aflp along with silver staining, a total of 136 transcript-derived fragments (tdfs) were found to be differentially expressed in response to challenge by u. scitaminea. forty tdfs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by rt-pcr analysis. these results demonstrated that the expressio ... | 2011 | 21792273 |
| sugarcane glycoproteins may act as signals for the production of xanthan in the plant-associated bacterium xanthomonas albilineans. | visual symptoms of leaf scald necrosis in sugarcane (saccharum officinarum) leaves develop in parallel to the accumulation of a fibrous material invading exocellular spaces and both xylem and phloem. these fibers are produced and secreted by the plant-associated bacterium xanthomonas albilineans. electron microscopy and specific staining methods for polysaccharides reveal the polysaccharidic nature of this material. these polysaccharides are not present in healthy leaves or in those from disease ... | 2011 | 21791980 |
| chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice. | chronic ethanol consumption is a major public health problem throughout the world. we investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice. | 2011 | 21789394 |
| development of a low-cost sterilization biological indicator using bacillus atrophaeus by solid-state fermentation. | the production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. the goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. the performance of three different inexpensive supports (vermiculite, sand, and sugarcane bagasse) was assessed by determining bacillus atrophaeus sporulation during solid-state fermentation and by assessing the direct u ... | 2011 | 21785930 |
| lipid production from yarrowia lipolytica po1g grown in sugarcane bagasse hydrolysate. | this study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (dscbh) as an alternative carbon source to culture yarrowia lipolytica po1g for microbial oil and biodiesel production. sugarcane bagasse hydrolysis with 2.5% hcl resulted in maximum total sugar concentration (21.38g/l) in which 13.59g/l is xylose, 3.98g/l is glucose, and 2.78g/l is arabinose. detoxification of scbh by ca(oh)(2) neutralization reduced the concentration of 5-hydroxymethylfurfural and fur ... | 2011 | 21757339 |
| poly-lactic acid synthesis for application in biomedical devices - a review. | bioabsorbable polymers are considered a suitable alternative to the improvement and development of numerous applications in medicine. poly-lactic acid (pla,) is one of the most promising biopolymers due to the fact that the monomers may produced from non toxic renewable feedstock as well as is naturally occurring organic acid. lactic acid can be made by fermentation of sugars obtained from renewable resources as such sugarcane. therefore, pla is an eco-friendly product with better features for u ... | 2011 | 21756992 |
| leviserpin: a serine peptidase inhibitor (serpin) from the sugarcane weevil sphenophorus levis. | serine peptidase inhibitors (serpins) form a superfamily of proteins covering abroad spectrum of different biological functions. here we describe the inhibitory characterization of leviserpin, the first serpin from the sugar cane weevil sphenophorus levis. leviserpin was able to inhibit bovine trypsin by the formation of the covalent complex serpin-peptidase, demonstrated by sds-page and mass spectroscopy analysis. we also have determined the cleavage site at the reactive center loop, by the ana ... | 2011 | 21748377 |
| production of bioethanol, methane and heat from sugarcane bagasse in a biorefinery concept. | the potential of biogas production from the residues of second generation bioethanol production was investigated taking into consideration two types of pretreatment: lime or alkaline hydrogen peroxide. bagasse was pretreated, enzymatically hydrolyzed and the wastes from pretreatment and hydrolysis were used to produce biogas. results have shown that if pretreatment is carried out at a bagasse concentration of 4% dm, the highest global methane production is obtained with the peroxide pretreatment ... | 2011 | 21689929 |
| high molecular variability of sugarcane bacilliform viruses in guadeloupe implying the existence of at least three new species. | thirty-five unique partial sugarcane bacilliform virus (scbv) sequences extending over 529bp were identified in sugarcane samples from guadeloupe diagnosed by immunocapture-pcr (ic-pcr) using specific badnavirus primers. phylogenetic analysis of these sequences along with the two known genome sequences of sugarcane bacilliform mor virus (scbmv) and sugarcane bacilliform im virus (scbimv) revealed high molecular variability in the scbv genome. seven phylogenetic groups, named a to g, were charact ... | 2011 | 21741419 |
| effects of d-003 (10 mg/day) on bone mineral density of the lumbar spine and femoral neck in postmenopausal women: a randomized, double-blinded study. | increased osteoclast activity is a pivotal finding in osteoporosis. this increase is mediated via the mevalonate-to-cholesterol pathway, which is involved in producing the intermediates required for osteoclast activity. d-003, a mixture of high molecular weight sugarcane wax acids, has been shown to inhibit cholesterol synthesis prior to mevalonate production, resulting in a reduction of bone loss and resorption in ovariectomized rats. moreover, previous studies have demonstrated that short-term ... | 2011 | 21716593 |
| plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania. | in sub-saharan africa, some individuals infected with malaria are also infected with helminths. however, the magnitude and distribution of such coinfections in relation to eco-systems remains poorly defined. this study was undertaken to determine the prevalence of plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania. | 2011 | 21741929 |
| production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid b, from sugarcane juice by ustilago scitaminea nbrc 32730. | mannosylerythritol lipids (mels) are glycolipid biosurfactants excreted by fungal strains. they show not only excellent surface-active properties but also versatile biochemical actions. ustilago scitaminea nbrc 32730 has been reported mainly to produce a mono-acetylated and di-acylated mel, mel-b, from sucrose as sole carbon source. in order to make biosurfactant production more efficient, we focused our attention on the use of sugarcane juice, one of the most economical resources. the fungal st ... | 2011 | 21737925 |
| complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of sugarcane mosaic virus. | sugarcane mosaic virus (scmv) is the most prevalent virus causing maize dwarf mosaic disease in northern china. a scmv isolate, bd8, was obtained from the maize showing dwarf and mosaic symptoms in baoding, china. the complete genomic sequence of bd8 is 9,576 nucleotides (nt) excluding the poly(a) tail. it contains one single open reading frame of 9,192-ánt and encodes a large polyprotein of 3,063-áamino acids (aa), flanked by a 5'-untranslated region (utr) of 148-ánt and a 3'-utr of 236-ánt. th ... | 2011 | 21833715 |
| differential protein expression in sugarcane during sugarcane-sporisorium scitamineum interaction revealed by 2-de and maldi-tof-tof/ms. | to understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. two sugarcane varieties, nco376 and ya71-374, were used in this study. by applying 2-dimensional electrophoresis (2-de), the protein expression profile of sugarcane after inoculating with sporisorium scitamineum was analyzed. in total, 23 differentially expressed proteins were identified by maldi-tof-tof/ms. bioinformatics analysis revealed t ... | 2011 | 21822403 |
| scientific challenges of bioethanol production in brazil. | bioethanol (fuel alcohol) has been produced by industrial alcoholic fermentation processes in brazil since the beginning of the twentieth century. currently, 432 mills and distilleries crush about 625 million tons of sugarcane per crop, producing about 27 billion liters of ethanol and 38.7 million tons of sugar. the production of bioethanol from sugarcane represents a major large-scale technology capable of producing biofuel efficiently and economically, providing viable substitutes to gasoline. ... | 2011 | 21735264 |
| multitemporal observations of sugarcane by terrasar-x images. | the objective of this study is to investigate the potential of terrasar-x (x-band) in monitoring sugarcane growth on reunion island (located in the indian ocean). multi-temporal terrasar data acquired at various incidence angles (17°, 31°, 37°, 47°, 58°) and polarizations (hh, hv, vv) were analyzed in order to study the behaviour of sar (synthetic aperture radar) signal as a function of sugarcane height and ndvi (normalized difference vegetation index). the potential of terrasar for mapping the ... | 2010 | 22163387 |
| comparative study of the areas of osteochondral defects produced in the femoral condyles of rabbits treated with gel of sugarcane biopolymer. | to measure the healed areas of osteochondral defects produced in femoral condyles of rabbits filled with biopolymer sugar cane gel and to compare these with those of the control group at 90, 120 and 180 days. | 2011 | 21952662 |
| Molecular Identification of Fusarium Species in Gibberella fujikuroi Species Complex from Rice, Sugarcane and Maize from Peninsular Malaysia. | The objective of this study was to identify Fusarium species in the Gibberella fujikuroi species complex from rice, sugarcane and maize as most of the Fusarium species in the species complex are found on the three crops. Isolates used were collected from the field and obtained from culture collection. The Fusarium isolates were initially sorted based on morphology and identifications confirmed based on the DNA sequence of the translation elongation factor 1-a (TEF-1a) gene. Based on the closest ... | 2011 | 22072914 |
| production of succinic acid from sucrose and sugarcane molasses by metabolically engineered escherichia coli. | sucrose-utilizing genes (csckb and csca) from escherichia coli ko11 were cloned and expressed in a metabolically engineered e. coli kj122 to enhance succinate production from sucrose. kj122 harboring a recombinant plasmid, pkjsuc, was screened for the efficient sucrose utilization by growth-based selection and adaptation. kj122-pkjsuc-24t efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. succinate concentrations of 51 ... | 2012 | 22023966 |