| isolation and identification of 3,6-dideoxy-3-(l-glyceroyl-amino)-d-glucose. a constituent of the antigenic polysaccharide of eubacterium saburreum, strain v5. | 3,6-dideoxy-3-(l-glyceroylamino)-d-glucose has been identified, for the first time, as a sugar component of the antigenic polysaccharide of eubacterium saburreum strain v5, principally by n.m.r. and mass spectrometry. | 1980 | 7407797 |
| biosynthesis of vitamin b12. experiments with the anaerobe eubacterium limosum and some labelled substrates. | 1. the anaerobic microorganism eubacterium limosum (dsm 20 402), grown in the presence of l-[methyl-14c]methionine, produces vitamin b12 not only labeled in the corrin ring, but also in the 5,6-dimethylbenzimidazole moiety. the vitamin b12 obtained in a similar experiment with l-[methyl-13c]methionine showed nine signals in the 13cv nmr spectrum. seven of these signals are due to the seven 'extra' methyl groups of the corrin ring, two signals originate from the two methyl groups of the 5,6-dimet ... | 1980 | 7408871 |
| surface vs core-tonsillar aerobic and anaerobic flora in recurrent tonsillitis. | specimens from both the surface and the core of tonsils from 23 children with recurrent tonsillitis were cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all patients. the predominant anaerobic isolates were bacteroides sp, fusobacterium nucleatum, anaerobic gram-positive cocci, and eubacterium sp. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, beta-hemolytic streptococci, and haemophilus sp. beta- ... | 1980 | 7411827 |
| structural studies of the eubacterium saburreum strain o 2 antigen. | | 1980 | 7417964 |
| role of alpha-tocopherolquinol in biohydrogenation. | | 1980 | 7422159 |
| isolation and characterization of fecal bacteria capable of 16 alpha-dehydroxylating corticoids. | for more than a decade it has been known that the fecal flora of humans and rats includes organisms capable of 16 alpha-dehydroxylating corticoids, but their identity has remained unknown. to isolate these organisms, mueller-hinton agar plates were seeded with fresh feces from proteus-free rats and incubated anaerobically. on an average, 1 of every 35 colonies consisted of organisms synthesizing 16 alpha-dehydroxylase. isolation of the individual colonies yielded two obligate anerobes, strains 1 ... | 1980 | 7425626 |
| agglutinins to bacteria in crohn's disease. | sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ... | 1980 | 7429299 |
| faecal bacterial flora in cancer of the colon. | selective aerobic and anaerobic plate media were employed to isolate the predominant faecal flora of patients with cancer of the colon (cc), cancer with non-gastrointestinal involvement (ngi), and with non-malignant diseases (n). the cc and n groups did not differ significantly in either total aerobic or anaerobic counts. the cc group did have a significantly lower anaerobic/aerobic ratio compared with the n group (2.42 vs. 2.96, p less than 0.05). this was the result of a greater predominance o ... | 1980 | 7429334 |
| effect of temperature, humidity and exposure to oxygen on the survival of anaerobic bacteria. | the relative effect of humidity, temperature and exposure to oxygen on the survival of 11 strains of bacteriodes spp., fusobacterium necrophorum, eubacterium lentum, clostridium ramosum, peptococcus asaccharolyticus and peptostreptococcus anaerobius was evaluated. suspensions of the test strains were applied to membrane filters either in empty sterile petri dishes or on plates of non-nutrient agar to provide dry or moist conditions. the filters were stored aerobically or anaerobically in an atmo ... | 1980 | 7431377 |
| 7 alpha-dehydroxylation of cholic acid by cell extracts of eubacterium species v.p.i. 12708. | nadh:flavin oxidoreductase and 7 alpha-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of eubacterium species v.p.i. 12708. assays of 7 alpha-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of nad+ plus fadh2. the ph optima of 7 alpha-dehydroxylase activity in whole cells and cell extracts were 7.0. the similar induction pattern of these two enzymes and the apparent cofactor requirements f ... | 1980 | 7435421 |
| fermentation of 1,2-o-iso-propylidene-d-glucofuranose ("monoacetone glucose") by anaerobic bacteria. | various species of clostridium, bacteroides, propionibacterium and eubacterium were incubated in a 1% solution of 1,2-o-iso-propylidene-d-glucofuranose in a peptone-yeast-extract (py) medium according to the vpi-technique. the volatile and non volatile acids were investigated by gas-liquid chromatography. all microorganisms showed a pattern of vfa different from that one produced by the glucose containing medium. in most microorganisms the formation of acetic acid was suppressed while the produc ... | 1980 | 7456850 |
| growth of cholesterol-reducing eubacterium on cholesterol-brain agar. | an agar medium containing 5% cholesterol has been developed for the isolation, enumeration, and characterization of cholesterol-reducing strains of eubacterium. | 1980 | 7458310 |
| exploring the mycoplasma capricolum genome: a minimal cell reveals its physiology. | we report on the analysis of 214kb of the parasitic eubacterium mycoplasma capricolum sequenced by genomic walking techniques. the 287 putative proteins detected to date represent about half of the estimated total number of 500 predicted for this organism. a large fraction of these (75%) can be assigned a likely function as a result of similarity searches. several important features of the functional organization of this small genome are already apparent. among these are (i) the expected relativ ... | 1995 | 7476192 |
| effect of proteolytic enzymes on the lysis and growth of oral bacteria. | previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ... | 1994 | 7478762 |
| a comparison of thermodynamic foldings with comparatively derived structures of 16s and 16s-like rrnas. | to increase our understanding of the dynamics and complexities of the rna folding process, and therewith to improve our ability to predict rna secondary structure by computational means, we have examined the foldings of a large number of phylogenetically and structurally diverse 16s and 16s-like rrnas and compared these results with their comparatively derived secondary structures. our initial goals are to establish the range of prediction success for this class of rrnas, and to begin comparing ... | 1995 | 7489516 |
| 16s rdna analysis reveals phylogenetic diversity among the polysaccharolytic clostridia. | small subunit rdna sequences were determined for 13 mesophilic, polysaccharolytic, mainly cellulolytic species of the genus clostridium and one cellulolytic eubacterium species. sequences were compared to those of 36 representatives of mesophilic and thermophilic clostridia, including those of nine thermophilic polysaccharolytic species published previously. the majority of strains group with 23s rrna clusters i and iii, while the others group with the thermophilic polysaccharolytic clostridia, ... | 1993 | 7505248 |
| experimental approaches for detecting self-splicing group i introns. | | 1993 | 7505383 |
| the presence of peptidoglycan-polysaccharide complexes in the bowel wall and the cellular responses to these complexes in crohn's disease. | interestingly, using a monoclonal antibody, peptidoglycan-polysaccharide complexes (ppc) were detected intracellularly in the mucosa and submucosa of the bowel wall of crohn's disease (cd) patients. ppc are the main constituents of the gram-positive bacterial cell wall. these ppc were however detected in the normal bowel wall also. therefore, in this study the hypothesis that an enhanced immune responsiveness to bacterial antigens plays a pivotal role in the induction or the chronicity of cd was ... | 1994 | 7515336 |
| two types of 16s rrna gene are found in campylobacter helveticus: analysis, applications and characterization of the intervening sequence found in some strains. | in the recently described species campylobacter helveticus, two sizes of pcr amplicon were detected with primers homologous to conserved regions of the 16s rrna gene. a conventionally sized gene was sequenced from the type strain, nctc 12470, placing the new species as phylogenetically related to c. upsaliensis and the thermotolerant campylobacters. this nucleotide sequence enabled pcr primers to be designed for use in rapid molecular identification of c. helveticus and its closest phylogenetic ... | 1994 | 7516795 |
| an intervening sequence (ivs) in the 16s rrna gene of the eubacterium helicobacter canis. | pcr amplicons enlarged by approximately 250bp were generated from the 16s rrna (rrs) genes of certain strains of the recently described helicobacter species, h. canis. the dna sequence of the rrs gene of one such strain was determined, and it was shown that an intervening sequence (ivs) of 235bp followed nucleotide 199 in the rrs sequence. in four other h. canis strains, identical or similar ivss were found, always at the same location in the rrs gene. the secondary structures of the rna transcr ... | 1994 | 7518076 |
| contribution of structural elements to thermus thermophilus ribonuclease p rna function. | we have performed a deletion and mutational analysis of the catalytic ribonuclease (rnase) p rna subunit from the extreme thermophilic eubacterium thermus thermophilus hb8. catalytic activity was reduced 600-fold when the terminal helix, connecting the 5' and 3' ends of the molecule, was destroyed by deleting 15 nucleotides from the 3' end. in comparison, the removal of a large portion (94 nucleotides, about one quarter of the rna) of the upper loop region impaired function only to a relatively ... | 1994 | 7525270 |
| techniques for controlling variability in gram staining of obligate anaerobes. | identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ... | 1995 | 7538512 |
| regulation of arylsulfate sulfotransferase from a human intestinal bacterium by nucleotides and magnesium ion. | arylsulfate sulfotransferase (asst) from a human intestinal bacterium stoichiometrically catalyzed the transfer of a sulfate group from phenylsulfate esters to phenolic compounds. pentachlorophenol, one of the selective inhibitors of phenol sulfoconjugation in mammalian tissues, inhibited both phenol and tyramine sulfation by asst. nucleotide triphosphates such as atp, gtp, utp and ctp, and pyrophosphate inhibited the asst activity, whereas mg2+ and mn2+ activated the enzyme and prevented its in ... | 1995 | 7542320 |
| evidence for the placement of the gram-negative catonella morbi (moore and moore) and johnsonella ignava (moore and moore) within the clostridium subphylum of the gram-positive bacteria on the basis of 16s rrna sequences. | comparative 16s rrna analysis was used to determine the phylogenetic positions of catonella morbi and johnsonella ignava, which are members of two monospecific genera of gram-negative anaerobic bacilli isolated from human gingival crevices. both of these genera were found to belong to cluster xiva (m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994) of the clostridium subphyl ... | 1995 | 7547310 |
| a retrospective review of cases of anaerobic empyema and update of bacteriology. | we conducted a retrospective study to update the bacteriology of 46 cases of anaerobic empyema that were originally studied between 1976 and 1993 at the wadsworth anaerobic bacteriology clinical research laboratory (los angeles). anaerobic bacteriologic studies were completed for all 46 pleural fluid specimens, and aerobic bacteriologic studies were completed for 41 of these specimens. thirty-seven clinical charts were available for review. a total of 161 anaerobic isolates (3.5 per patient) rep ... | 1995 | 7548560 |
| phosphorylation in halobacterial signal transduction. | regulated phosphorylation of proteins has been shown to be a hallmark of signal transduction mechanisms in both eubacteria and eukarya. here we demonstrate that phosphorylation and dephosphorylation are also the underlying mechanism of chemo- and phototactic signal transduction in archaea, the third branch of the living world. cloning and sequencing of the region upstream of the chea gene, known to be required for chemo- and phototaxis in halobacterium salinarium, has identified chey and cheb an ... | 1995 | 7556066 |
| motility and thermotactic responses of thermotoga maritima. | thermotoga maritima, a thermophilic eubacterium, is motile at temperatures ranging from 50 to 105 degrees c. the cells are propelled by a single flagellum which most of the time spins clockwise. changes in the swimming direction ("tumbles") are achieved by short reversals of the direction of filament rotation. the average speed of swimming cells depends on the temperature, reaching a maximum value of about 60 microns/s at 85 degrees c. the cells show a thermotactic response to temporal temperatu ... | 1995 | 7559332 |
| [in vitro activity of carbapenems (biapenem, imipenem and meropenem) and some other antibiotics against strict anaerobic bacteria]. | during 1994, the in vitro antibiotic susceptibility of 306 anaerobic bacteria was performed in 4 hospitals, by the reference agar dilution method. among the 129 b. fragilis group strains, only two b. fragilis strains were resistant to the three carbapenems and all beta-lactams, even combined with beta-lactamase-inhibitors while metronidazole resistance could not be detected. evolution in antibiotic resistance rates could be assessed only for piperacillin whose resistance rates increased to 20%. ... | 1995 | 7567117 |
| a nuclear gene of eubacterial origin in euglena gracilis reflects cryptic endosymbioses during protist evolution. | genes for glycolytic and calvin-cycle glyceraldehyde-3-phosphate dehydrogenase (gapdh) of higher eukaryotes derive from ancient gene duplications which occurred in eubacterial genomes; both were transferred to the nucleus during the course of endosymbiosis. we have cloned cdnas encoding chloroplast and cytosolic gapdh from the early-branching photosynthetic protist euglena gracilis and have determined the structure of its nuclear gene for cytosolic gapdh. the gene contains four introns which pos ... | 1995 | 7568085 |
| ruminal microbial digestion in free-living, in captive lichen-fed, and in starved reindeer (rangifer tarandus tarandus) in winter. | in free-living (fl) reindeer eating a natural mixed winter diet dominated by lichens, captive (cf) reindeer fed pure lichens ad libitum, and cf reindeer subsequently starved for 1 day (cs1 reindeer) or 4 days (cs4 reindeer), the dominant rumen anaerobic bacteria were characterized, their population densities were estimated, and ruminal ph and volatile fatty acid concentrations were determined. in the fl reindeer, the total median viable anaerobic bacterial population ranged from 18 x 10(8) to 35 ... | 1995 | 7574599 |
| intestinal floras of populations that have a high risk of colon cancer. | the fecal floras of polyp patients, japanese-hawaiians, north american caucasians, rural native japanese, and rural native africans were compared. the polyp patients and japanese-hawaiians were considered to be groups at high risk of colon cancer, and the rural native japanese and rural native africans were considered to be groups at low risk. the north american caucasians were found to have a flora composition intermediate between these two groups. fifteen bacterial taxa from the human fecal fl ... | 1995 | 7574628 |
| cloning and characterization of senc, a gene involved in both aerobic respiration and photosynthesis gene expression in rhodobacter capsulatus. | the purple nonsulfur photosynthetic eubacterium rhodobacter capsulatus is a versatile organism that can obtain cellular energy by several means, including the capture of light energy for photosynthesis as well as the use of light-independent respiration, in which molecular oxygen serves as a terminal electron acceptor. in this study, we have identified and characterized a novel gene, senc, mutations in which affect respiration as well as the induction of photosynthesis gene expression. the prote ... | 1995 | 7592491 |
| cellular source, activation and inhibition of dental plaque collagenase. | dental plaque is the major aetiological factor in periodontal diseases and contains several proteolytic enzymes. the origin of these proteinases is, however, poorly studied. this study was undertaken to characterize collagenase present in dental plaque of adult periodontitis patients. vertebrate-type rather than bacterial-derived collagenase activity was detected in extracts of both supra- and subgingival dental plaque extracts of adult periodontitis patients. dental plaque collagenase was found ... | 1995 | 7593702 |
| factors affecting the occurrence of bacteremia associated with tooth extraction. | this study examined the factors that affect the occurrence of bacteremia associated with tooth extraction and the kinds of bacteria causing this bacteremia. bacteremia was found in 132 (72.1%) of 183 patients who had one or more teeth extracted for various reasons. bacteremia occurred more frequently when teeth were extracted because of inflammatory dental diseases. the occurrence of bacteremia also increased with the number of teeth extracted and the age of the patients. when the volume of bloo ... | 1995 | 7594761 |
| nucleotide sequence and chromosomal localization of the gene encoding the old yellow enzyme from kluyveromyces lactis. | a 6.6 kb genomic dna fragment from the yeast kluyveromyces lactis was isolated. sequence analysis of this fragment revealed the presence of two incomplete open reading frames (orfs) in one strand, one coding for the carboxyl terminus of the plasma membrane h(+)-atpase and the other for the amino terminus of an unidentified product. in the complementary strand, a full-length orf which encodes for a protein homologous to the yeast nadph-dependent old yellow enzyme was found. the deduced amino acid ... | 1995 | 7597850 |
| expression of the bile acid-inducible nadh:flavin oxidoreductase gene of eubacterium sp. vpi 12708 in escherichia coli. | the intestinal microorganism eubacterium sp. vpi 12708 synthesizes a bile acid-inducible nadh:flavin oxidoreductase (nadh:for) which presumably functions in the 7 alpha-dehydroxylation of cholic acid to deoxycholic acid. the baih gene encoding nadh:for was subcloned into an iptg-inducible expression vector, pbaih2.2. escherichia coli dh5 alpha cells transformed with pbaih2.2 expressed 10-fold higher levels of nadh:for upon induction with iptg than did eubacterium sp. vpi 12708 cells induced with ... | 1995 | 7599167 |
| the effect of a probiotic on faecal and liver lipid classes in rats. | the effect of a probiotic composed of bacillus, lactobacillus, streptococcus, saccharomyces and candida species (each at 10(7-8) colony-forming units (cfu)/g rice bran), given at a level of 150 g/kg diet for 6 weeks, on lipid metabolism was examined in the faeces, serum and liver of male rats. liver weight decreased 35% in the rats fed on a high-fat, high-cholesterol diet containing the probiotic. total cholesterol concentration in the serum was significantly lower in the probiotic group than in ... | 1995 | 7626589 |
| [nosocomial infections in present-day traumatological-orthopedic hospitals]. | nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ... | 1995 | 7626997 |
| a thermolabile triosephosphate isomerase from the psychrophile vibrio sp. strain ant-300. | we report the isolation of a gene encoding triosephosphate isomerase (tim; ec 5.3.1.1) from vibrio sp. strain ant-300, a psychrophilic marine eubacterium that grows optimally at 7 degrees c. the deduced primary sequence of this isomerase is 50% identical to escherichia coli tim and 37% identical to the isomerase of the psychrotroph moraxella sp. ta137. transformation with this gene allowed growth of a tim-deficient e. coli strain on selective media, but only at temperatures below 30 degrees c. t ... | 1995 | 7639513 |
| bacteremia in conjunction with endodontic therapy. | this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ... | 1995 | 7641631 |
| convergent evolution of amino acid usage in archaebacterial and eubacterial lineages adapted to high salt. | chemical composition and physical properties of the total protein of haloferax mediterranei, a halophilic archaebacterium requiring high salt concentration for growth, of halomonas elongata, a halotolerant eubacterium able to grow at any concentration of salt, and of escherichia coli b, a eubacterium related to h. elongata, unable to grow at high salt concentration, were compared using robust standard biochemical methods. the distribution of amino acid abundancies in the bulk protein from h. elo ... | 1995 | 7652205 |
| [serologic examinations for the detection of antibodies against eubacterium suis in swine]. | determination of antibodies against eubacterium suis (e. suis) in the serum of pigs was carried out by an indirect immunofluorescence test. therefore 35 sows with urinary tract infections as well as 43 healthy control sows were investigated. e. suis could be detected in the urine of 19 sows. serological results were compared with the bacteriological examinations. to reach a specificity of 100% for detection of animals actually infected with e. suis, titres from 1:16++ can be accepted as positive ... | 1995 | 7660097 |
| interaction of the rumen fungus orpinomyces joyonii with megasphaera elsdenii and eubacterium limosum. | the degradation and fermentation of microcrystalline cellulose were studied in monoculture of the polycentric anaerobic fungus orpinomyces joyonii and in co-cultures with the rumen bacteria megasphaera elsdenii and eubacterium limosum. more than 25% of cellulose hydrolysis products (glucose and cellodextrins) were released by the fungus into the medium after 8 d of cultivation. these products were metabolized by bacteria in mixed cultures. in co-culture with the fungus m. elsdenii and e. limosum ... | 1995 | 7662333 |
| refolding of glutamate dehydrogenase from bacillus acidocaldarius after guanidinium chloride-induced unfolding. | the hexameric nad-dependent glutamate dehydrogenase from the thermophilic eubacterium bacillus acidocaldarius is the first glutamate dehydrogenase which spontaneously refolds in vitro. after 24 h unfolding in 6 m guanidinium chloride at 20 degrees c, refolding was achieved by dilution of the denaturant. the yield of reconstitution obtained in the presence of 1,4 dithio-dl-threitol in the unfolding/refolding mixture was 75%. the unfolding/refolding equilibria have been studied by fluorescence, ci ... | 1995 | 7663395 |
| identification of proteolytic rumen bacteria isolated from new zealand cattle. | the protease activities of 212 strains of rumen bacteria isolated from new zealand cattle grazing pasture were measured. thirty-seven per cent of strains had activity greater than or equal to the proteolytic rumen bacterium prevotella ruminicola and 43 of these isolates were identified by morphology, carbon source utilization, gram stain, biochemical tests and fermentation end-product analysis. hierarchical cluster analysis showed that the strains formed four clusters: cluster a contained 26 str ... | 1995 | 7665388 |
| structure of peptidoglycan from thermus thermophilus hb8. | the composition and structure of peptidoglycan (murein) extracted from the extreme thermophilic eubacterium thermus thermophilus hb8 are presented. the structure of 29 muropeptides, accounting for more than 85% of total murein, is reported. the basic monomeric subunit consists of n-acetylglucosamine-n-acetylmuramic acid-l-ala-d-glu-l-orn-d-ala-d-ala, acylated at the delta-nh2 group of orn by a gly-gly dipeptide. in a significant proportion (about 23%) of total muropeptides, the n-terminal gly is ... | 1995 | 7665471 |
| detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease. | the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ... | 1995 | 7675524 |
| the metabolism of phenylalanine and leucine by a cell suspension of eubacterium brachy and the effects of metronidazole on metabolism. | degradation of phenylalanine and leucine by resting cells of eubacterium brachy atcc 33089 were studied under strict anaerobic conditions. the effects of metronidazole and air on the metabolism were also studied. the organism principally produced phenylpropionate and isocaproate from phenylalanine and leucine, respectively. other products were cinnamate and phenylacetate from phenylalanine, and alpha-ketoisocaproate and isovalerate from leucine. the organism also produced hydroxylated end-produc ... | 1994 | 7695510 |
| mode of transformation of [1-15n]5-hydroxybenzimidazole and [1-15n]5-hydroxy-6-methylbenzimidazole into the 5,6-dimethylbenzimidazole moiety of vitamin b12. | the transformation of [1-15n]5-hydroxybenzimidazole and [1-15n]5-hydroxy-6-methylbenzimidazole into the 5,6-dimethylbenzimidazole moiety of vitamin b12 by eubacterium limosum-cultures was studied. the vitamin b12 obtained was exclusively 15n-labeled in n-1 of the base part, as revealed by nmr-measurements. this indicates that either the unsubstituted 5,6-dimethylbenzimidazole presumably formed is not released from the enzyme until the ribose-5'-phosphate substituent is introduced, or that the pr ... | 1994 | 7695841 |
| positive and negative associations between bacterial species in dental root canals. | significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ... | 1994 | 7700162 |
| phylogenetic analysis of 70 kd heat shock protein sequences suggests a chimeric origin for the eukaryotic cell nucleus. | the evolutionary relationships between archaebacteria, eubacteria and eukaryotic cells are of central importance in biology. the current view is that each of these three groups of organisms constitutes a monophyletic domain, and that eukaryotic cells have evolved fom an archaebacterial ancestor. recent studies on a number of highly conserved protein sequences do not, however, support this view and raise important questions concerning the evolutionary relationships between all extant organisms, p ... | 1994 | 7704574 |
| of archae and eo: what's in a name? | | 1995 | 7708656 |
| similarity of "core" structures in two different glycans of tyrosine-linked eubacterial s-layer glycoproteins. | previously, the repeating-unit structure of the s-layer glycoprotein from the eubacterium bacillus alvei ccm 2051 has been determined to be [-->3)-beta-d-galp-(1-->4)-[alpha-d-glcp-(1-->6)-]-beta-d-manpnac- (1-->]n (e. altman, j.-r. brisson, p. messner, and u. b. sleytr, biochem. cell biol. 69:72-78, 1991). nuclear magnetic resonance spectroscopic reexamination of this glycan reveals that the o-antigen-like domain of the polysaccharide is [see text] connected with the s-layer polypeptide through ... | 1995 | 7721708 |
| model structure of the omp alpha rod, a parallel four-stranded coiled coil from the hyperthermophilic eubacterium thermotoga maritima. | omp alpha is an outer-membrane protein that spans the periplasmic space of the hyperthermophilic eubacterium thermotoga maritima. the molecule contains a globular head with an apparent diameter of 8 nm and a rod-shaped tail of 40 nm length. the sequence of the globular domain is homologous to a conserved region of cell wall-bound proteins and probably attaches omp alpha to the peptidoglycan. the sequence of the rod domain resembles that of coiled coil proteins and ends in a transmembrane segment ... | 1995 | 7731042 |
| a prokaryotic origin for light-dependent chlorophyll biosynthesis of plants. | flowering plants require light for chlorophyll synthesis. early studies indicated that the dependence on light for greening stemmed in part from the light-dependent reduction of the chlorophyll intermediate protochlorophyllide to the product chlorophyllide. light-dependent reduction of protochlorophyllide by flowering plants is contrasted by the ability of nonflowering plants, algae, and photosynthetic bacteria to reduce protochlorophyllide and, hence, synthesize (bacterio) chlorophyll in the da ... | 1995 | 7731978 |
| enzymic studies on the animal and intestinal bacterial metabolism of geniposide. | geniposide, a main iridoid glucoside of gardenia fruit, is transformed to genipin, a genuine choleretic, in vivo in rats (aburada et al., j. pharmacobio-dyn., 1, 81 (1978)). as geniposide was not hydrolyzed to any metabolite by rat liver homogenate, which has beta-d-glucosidase and esterase activities, beta-d-glucosidases in intestinal bacteria seem to be required for an exhibition of its choleretic action. the crude extract of eubacterium sp. a-44, a human intestinal anaerobe, hydrolyzed genipo ... | 1994 | 7735197 |
| effect of h2-receptor antagonists on bile acid metabolism. | several reports have been presented concerning pronounced overgrowth of bacteria in gastric juices of patients treated with h2-receptor antagonists. however, there has been no report concerning influence of h2-receptor antagonists on jejunal flora. thus, to investigate the influence and its effect on bile acid metabolism, this study was performed: 1) to examine whether patients with gastric ulcers who have been treated with h2-receptor antagonists have positive bile acid breath tests due to bact ... | 1995 | 7735920 |
| glycine metabolism in anaerobes. | some strict anaerobic bacteria catalyze with glycine as substrate an internal stickland reaction by which glycine serves as electron donor being oxidized by glycine-cleavage system or as electron acceptor being reduced by glycine reductase. in both cases, energy is conserved by substrate level phosphorylation. except for the different substrate-activating proteins pb, reduction of sarcosine or betaine to acetyl phosphate involves in eubacterium acidaminophilum the same set of proteins as observe ... | 1994 | 7747933 |
| congruent evolution between whiteflies (homoptera: aleyrodidae) and their bacterial endosymbionts based on respective 18s and 16s rdnas. | whiteflies (family aleyrodidae) possess heritable eubacterial endosymbionts sustained in specialized organ-like structure called mycetomes. comparisons of distances between the ash whitefly, siphoninus phillyreae, and two biotypes ("a" and "b") of the sweetpotato whitefly, bemisia tabaci, based on sequence analysis of genes for 18s rrnas (rdnas), were equivalent to the distances represented by the 16s rdnas of their respective endosymbionts. this finding indicates that evolutionary divergence in ... | 1993 | 7763374 |
| anaerobic biodegradation of methyl esters by acetobacterium woodii and eubacterium limosum. | the ability of acetobacterium woodii and eubacterium limosum to degrade methyl esters of acetate, propionate, butyrate, and isobutyrate was examined under growing and resting-cell conditions. both bacteria hydrolyzed the esters to the corresponding carboxylates and methanol under either condition. methanol was further oxidized to formate under growing but not resting conditions. unlike the metabolism of phenylmethylethers, no h2 requirement was evident for ester biotransformation. the hydrolysis ... | 1994 | 7765371 |
| hypocholesterolemic effect of eubacterium coprostanoligenes atcc 51222 in rabbits. | recently, a unique bacterium, eubacterium coprostanoligenes atcc 51222, that reduces cholesterol to coprostanol was isolated. because coprostanol is absorbed poorly, it was hypothesized that oral administration of eu. coprostanoligenes might decrease cholesterol concentration in blood because the micro-organisms will decrease the absorption of endogenous and dietary cholesterol by conversion to coprostanol. to test the hypothesis, three adult new zealand white rabbits received 4 ml of eu. copros ... | 1995 | 7766068 |
| expression in escherichia coli and characterization of a bile acid-inducible 3 alpha-hydroxysteroid dehydrogenase from eubacterium sp. strain vpi 12708. | we have previously cloned and sequenced three members of a bile acid-inducible gene family from eubacterium sp. strain vpi 12708 that encode 27,000-m(r) polypeptides. two copies of these genes (baia1 and baia3) are identical, while the third copy (baia2) encodes a polypeptide sharing 92% amino acid identity with the baia1 and baia3 gene products. we have overexpressed the baia1 gene in escherichia coli and analyzed the expressed activity. thin-layer chromatography of 14c-labeled bile acid produc ... | 1995 | 7766153 |
| dna repair mutants of rhodobacter sphaeroides. | the genome of the photosynthetic eubacterium rhodobacter sphaeroides 2.4.1 comprises two chromosomes and five endogenous plasmids and has a 65% g+c base composition. because of these characteristics of genome architecture, as well as the physiological advantages that allow this organism to live in sunlight when in an anaerobic environment, the sensitivity of r. sphaeroides to uv radiation was compared with that of the more extensively studied bacterium escherichia coli. r. sphaeroides was found ... | 1995 | 7768798 |
| purification, cloning, and sequencing of archaebacterial pyrophosphatase from the extreme thermoacidophile sulfolobus acidocaldarius. | cytoplasmic pyrophosphatases are indispensible for the function of cellular bioenergetics. from the extreme thermoacidophilic archaeon sulfolobus acidocaldarius, situated at one of the lowest branches of the phylogenetic tree, a cytosolic pyrophosphatase has been isolated and purified 200-fold to electrophoretic homogeneity by combining ion-exchange and gel-exclusion chromatography. the native enzyme consists of a homotetramer of 71 kda apparent molecular mass; the subunit displays an apparent m ... | 1995 | 7771779 |
| [the therapy of urinary tract infections in sows]. | fifteen sows spontaneously infected with eubacterium suis were treated with enrofloxacin and, if necessary, with ampicillin. before and after treatment different blood and urine parameters were determined with regard to renal function and the condition of the urinary bladder was examined by cystoscopy. besides results of a controlled treatment in a breeding herd with a high frequency of urinary tract infections are referred. duration of treatment with appropriate dosage should be at least 10 day ... | 1995 | 7781534 |
| transposon mutagenesis in halophilic eubacteria: conjugal transfer and insertion of transposon tn5 and tn1732 in halomonas elongata. | molecular genetic studies of halophilic eubacteria have been limited by the lack of a suitable method for mutagenesis. to overcome this, we established a transposon mutagenesis procedure for the ectoine-producing, halophilic bacterium halomonas elongata. we used suicide plasmids psup101 and psup102-gm to introduce the transposons tn5 and tn1732 respectively into h. elongata via escherichia coli sm10 mediated conjugation. our finding that h. elongata is sensitive to aminoglycoside antibiotics at ... | 1995 | 7781978 |
| isolation and characterization of bile acid 7-dehydroxylating bacteria from human feces. | methods for isolation of fecal 7 alpha-dehydroxylating bacteria are presented. a total of 219 strains were isolated from feces of healthy humans, and their ability to 7-dehydroxylate cholic, chenodeoxycholic, and ursodeoxycholic acids were examined. of all the isolates, 14 strains were found to be capable of eliminating the hydroxy group at c-7 alpha and/or c-7 beta. all the isolates were strictly anaerobic, gram-positive rods. thirteen isolates were non-sporeforming bacteria showing certain sac ... | 1995 | 7783673 |
| effects of the lipid-saccharide complex and unsaponifiable matter from sunflowers on liver lipid metabolism and intestinal flora in rats. | the effects of the flower lipid-saccharide complex and unsaponifiable matter (1 g/kg of diet) from the sunflower on liver lipid metabolism and intestinal flora was studied in rats given cholesterol-enriched diets. after six weeks of feeding, the microsomal cholesterol concentration in the liver had been significantly reduced with the sunflower diet. the ratio of cholesterol/phospholipid was also reduced by the sunflower diet. the 3-hydroxy-3-methylglutaryl coenzyme a reductase activity of the su ... | 1995 | 7787300 |
| abnormal colonic microbial function in patients with rheumatoid arthritis. | the aim of this study was to examine the microflora-associated characteristics (macs) of faecal samples of patients with rheumatoid arthritis (ra) and to evaluate the actions of sulphasalazine (sasp) on these macs. the conversion of cholesterol to coprostanol, the production of urobilinogen, the degradation of faecal tryptic activity (fta) and of beta-aspartylglycine were measured in faecal samples from 19 patients treated with sasp and 21 patients not treated with this medication. a control gro ... | 1994 | 7801055 |
| purification, properties and structural aspects of a thermoacidophilic alpha-amylase from alicyclobacillus acidocaldarius atcc 27009. insight into acidostability of proteins. | the alpha-amylase from the thermoacidophilic eubacterium alicyclobacillus (bacillus) acidocaldarius strain atcc 27009 was studied as an example of an acidophilic protein. the enzyme was purified from the culture fluid. on an sds/polyacrylamide gel, the protein an apparent molecular mass of 160 kda, which is approximately 15% higher than that predicted from the nucleotide sequence. the difference is due to the enzyme being a glycoprotein. deglycosylation or synthesis of the enzyme in escherichia ... | 1994 | 7813489 |
| absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium. | to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ... | 1994 | 7814703 |
| enzymatic sulfation of glycosides and their corresponding aglycones by arylsulfate sulfotransferase from a human intestinal bacterium. | a novel type of arylsulfate sulfotransferase (asst) from a predominant human intestinal bacterium catalyzes the stoichiometric transfer of a sulfate group from phenolic sulfate esters to phenols. we clarified that polyphenols were better substrates of this enzyme than the corresponding glycosides. additionally, a coumarin derivative, esculetin, was sulfated by asst at the 6-position to give 6-monosulfate. therefore, asst is more useful for the preparation of sulfated polyphenols at their specifi ... | 1994 | 7820100 |
| splenic granulomas in broiler chickens produced experimentally by inoculation with eubacterium tortuosum. | eubacterium tortuosum, a gram-positive anaerobic filamentous bacillus, was isolated from splenic and hepatic granulomas of a 56-day-old slaughtered chicken. this isolate was injected intravenously into two groups of 2-week-old broiler chickens, which were necropsied 19 days later. five of 15 chickens injected with 5 x 10(6) colony-forming units of a 48-hour culture of e. tortuosum developed splenic granulomas typical of those seen in chickens at slaughter. no lesions were observed in chickens gi ... | 1994 | 7832715 |
| increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes. | a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ... | 1994 | 7851086 |
| expression of rat liver adohcy hydrolase in a rhodobacter capsulatus ahcy mutant restores pigment formation and photosynthetic growth. | an amino acid alignment of fourteen s-adenosylhomocysteine hydrolases shows that sequences from six photosynthetic species and one species possibly derived from algae have an internal 36 to 41 amino acid sequence that is not present in hydrolase sequences from seven non-photosynthetic species. in the photosynthetic eubacterium rhodobacter capsulatus, the stlb1 strain has a disrupted hydrolase gene, and hydrolase activity is not detectable. photopigment synthesis and photosynthetic growth are sig ... | 1995 | 7857275 |
| the gingival immune response to periodontal pathogens in juvenile periodontitis. | a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ... | 1994 | 7870467 |
| degradation of immunoglobulin g by periodontal bacteria. | several subgingival microorganisms were tested for their ability to utilize human immunoglobulin g (igg) as a substrate for growth. this was done using a protein-free chemically defined medium, supplemented with igg. stimulation of growth was observed for capnocytophaga ochracea, porphyromonas asaccharolytica, porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, prevotella oralis, lactobacillus catenaforme and streptococcus intermedius. immunoelectrophoresis, sodium dodec ... | 1994 | 7870469 |
| establishment of a microbiologically acceptable daily intake of antimicrobial drug residues. | a model is presented to calculate the microbiologically acceptable daily intake (adim) of antibiotic residues in food products. the adim calculation is based on mic values for indicator bacteria escherichia coli, bacteroides fragilis, bifidobacterium spp. and eubacterium spp., established under gut-like conditions in an in vitro simulation model. the maximum residue level (mrl) for residues in food products can be derived from the adim. four phases can be distinguished in this gastro-intestinal ... | 1994 | 7871699 |
| sulfation of parabens and tyrosylpeptides by bacterial arylsulfate sulfotransferases. | arylsulfate sulfotransferase purified from eubacterium a-44 has higher specific activity than the enzymes from klebsiella k-36 and haemophilus k-12. propylparaben and butylparaben were good substrates among several parabens. the antibacterial activity of parabens was reduced by the sulfation of the phenolic hydroxy group. tyrosine-containing peptides, kyotorphin, enkephalin and cholecystokinin non-sulfate, were effective as acceptor substrates by a-44, k-36 and k-12 sulfotransferases. | 1994 | 7874051 |
| major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes. | two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ... | 1994 | 7874880 |
| ubiquitin in the prokaryote anabaena variabilis. | the ubiquitin-dependent pathway for protein degradation has been found to play a major role in controlling protein turnover in the cell. ubiquitin is one of the most conserved proteins yet identified, and up until now it has been thought to be present only in eukaryotes and archaebacteria. this is the first report on the detection and purification of ubiquitin from a eubacterium, the cyanobacterium anabaena variabilis. the purification procedure included a heat denaturing step, fractionated ammo ... | 1995 | 7876111 |
| altered monovinyl and divinyl protochlorophyllide pools in bchj mutants of rhodobacter capsulatus. possible monovinyl substrate discrimination of light-independent protochlorophyllide reductase. | in land plants in particular, it has been well established that chlorophyll intermediates, mg-protoporphyrin, mg-protoporphyrin monomethylester, protochlorophyllide, and chlorophyllide occur as monovinyl and divinyl forms. the pool of monovinyl and divinyl intermediates differ according to species, age of tissue, and light regime. in this study, we investigated the monovinyl and divinyl characteristics of protochlorophyllide and chlorophyllide in the purple non-sulfur photosynthetic eubacterium ... | 1995 | 7876113 |
| [effect of biapenem (l-627) on fecal flora in gnotobiotic mice and children]. | biapenem (l-627), a novel injectable carbapenem antibiotic, was studied with regard to its effect on mice inoculated with four types of bacteria and on the intestinal flora of pediatric patients. l-627 was given i.m., 40 mg/kg once daily for 5 consecutive days, to mice inoculated enterically with four types of bacteria (escherichia coli, enterococcus faecalis, bacteroides fragilis, and bifidobacterium breve). except for a mild decrease in e. coli, there were no major fluctuations in viable bacte ... | 1994 | 7877249 |
| assessing horizontal transfer of nifhdk genes in eubacteria: nucleotide sequence of nifk from frankia strain hfpcci3. | the structural genes for nitrogenase, nifk, nifd, and nifh, are crucial for nitrogen fixation. previous phylogenetic analysis of the amino acid sequence of nifh suggested that this gene had been horizontally transferred from a proteobacterium to the gram-positive/cyanobacterial clade, although the confounding effects of paralogous comparisons made interpretation of the data difficult. an additional test of nif gene horizontal transfer using nifd was made, but the nifd phylogeny lacked resolution ... | 1995 | 7877490 |
| cellular fatty acids and aldehydes of oral eubacterium. | the cellular fatty acids and aldehydes of oral eubacterium species were determined by gas chromatography-mass spectrometry. e. brachy and e. lentum contained mainly branched-chain fatty acids, whereas the others contained straight-chain acids. e. brachy, e. lentum, e. yurii ssp. yurii, e. yurii spp. margaretiae, e. limosum, e. plauti and e. aerofaciens also contained aldehydes with even carbon numbers. in addition to species-specific components, the compositional ratios of fatty acids and aldehy ... | 1995 | 7896079 |
| origin of d-alanine present in urine of mutant mice lacking d-amino-acid oxidase activity. | urine of mutant ddy/dao- mice lacking d-amino-acid oxidase contained 13 times more d-alanine than that of normal ddy/dao+ mice. because d-alanine is a component of bacterial cell walls, the possibility that the urinary d-alanine came from intestinal bacteria was examined. in ddy/dao- mice that were made germ free at birth and reared in a germ-free environment, the quantity of urinary d-alanine was found to be at a low level comparable to that of the normal mice. when these germ-free mice were ma ... | 1993 | 7901999 |
| anaerobic susceptibility testing. slight differences in inoculum size can make a difference in minimum inhibitory concentrations. | in the present study, we compared the actual inoculum density from inoculated broth microdilution wells to the targeted inoculum size (10(5) cfu/well) when the inoculum was prepared using a mcfarland nephelometer to achieve the standard density. three target inoculum sizes (10(5), 5 x 10(5), and 10(6) cfu/well) were used to compare the effect of slight inoculum size increases of both atcc and clinical strains of anaerobes on mics of various antimicrobials. actual colony counts of bacteroides fra ... | 1994 | 7924212 |
| cloning and sequencing of a rhodothermus marinus gene, bgla, coding for a thermostable beta-glucanase and its expression in escherichia coli. | a gene library of the thermophilic eubacterium, rhodothermus marinus, strain 21, was prepared in puc18 and used to transform escherichia coli. of 5400 transformants, two produced halos on lichenan plates after congo-red staining. restriction mapping showed that the two clones shared an overlapping 1200-bp dna fragment, which was used for dna sequencing. five potential methionine (met) translational-initiation codons were identified. a putative signal peptide of 30 amino acids was identified with ... | 1994 | 7925416 |
| the sequence of the single 16s rrna gene of the thermophilic eubacterium rhodothermus marinus reveals a distant relationship to the group containing flexibacter, bacteroides, and cytophaga species. | rhodothermus marinus, a gram-negative heterotrophic marine thermophile, has been the subject of several recent studies. isolation, sequencing, and analyses of a 16s rrna gene have shown that r. marinus diverges sharply from major bacterial phyla and is most closely allied to the flexibacter-cytophaga-bacteroides group. further analyses revealed that the r. marinus chromosome contains a single rrna operon with a 16s-23s intergenic region coding for trna(ile) and trna(ala). | 1994 | 7928984 |
| detection of intestinal flora-derived bacterial antigen complexes in splenic macrophages of rats. | we studied the presence of bacterial antigens in rat tissues. we produced a monoclonal antibody (mab 2e9) directed against intestinal flora-derived peptidoglycan-polysaccharide complexes from human and rat feces. with several immunological techniques, the specificity of 2e9 for this bacterial product was demonstrated. using 2e9 in an immunohistological assay, we were able to show the presence of bacterial products in macrophages in the red pulp of spleens of conventional lewis rats. however, we ... | 1994 | 7930525 |
| from initiation to elongation: comparison of transcription by prokaryotic and eukaryotic rna polymerases. | multisubunit rna polymerases in prokaryotes and eukaryotes share an evolutionarily conserved core. here, we compare the processes of promoter recognition, transcription initiation and transcript elongation by human rna polymerase ii and by the rna polymerase of the eubacterium escherichia coli. although these two polymerases have diverged widely in structure, important functions have been conserved, suggesting that the basic mechanisms of rna transcription are similar in eukaryotes and prokaryot ... | 1994 | 7940759 |
| variations in concentrations of bacterial metabolites, enzyme activities, moisture, ph and bacterial composition between and within individuals in faeces of seven healthy adults. | variations between and within individuals, and correlations between concentrations of bacterial metabolites, including putrefactive products, ammonia and short chain fatty acids (scfas), enzyme activities, moisture and ph, as well as bacterial composition, were studied in faecal samples from seven healthy adults over a period of 7 months. large variations, both between and within individuals, were observed in concentrations of putrefactive products. although values for ammonia, scfas, enzyme act ... | 1994 | 7961190 |
| cloning, dna sequencing, and characterization of a nifd-homologous gene from the archaeon methanosarcina barkeri 227 which resembles nifd1 from the eubacterium clostridium pasteurianum. | l. sibold, m. henriquet, o. possot, and j.-p. aubert (res. microbiol. 142:5-12, 1991) cloned and sequenced two nifh-homologous open reading frames (orfs) from methanosarcina barkeri 227. phylogenetic analysis of the deduced amino acid sequences of the nifh orfs from m. barkeri showed that nifh1 clusters with nifh genes from alternative nitrogenases, while nifh2 clusters with nifh1 from the gram-positive eubacterium clostridium pasteurianum. the n-terminal sequence of the purified nitrogenase com ... | 1994 | 7961410 |
| increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria. | a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ... | 1994 | 7979307 |
| the phylogeny of the genus clostridium: proposal of five new genera and eleven new species combinations. | the 16s rrna gene sequences of 34 named and unnamed clostridial strains were determined by pcr direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- g + c-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of the clostridia. the results of our phylogenetic analysis corroborate and extend previous findings in show ... | 1994 | 7981107 |
| improved purification of arylsulfate sulfotransferase from human intestinal bacterium by using polyclonal antibody. | arylsulfate sulfotransferase (asst) from a human intestinal bacterium stoichiometrically catalyzed the transfer of the sulfate group of phenylsulfate esters to phenolic compounds. polyclonal antibodies against asst were obtained from rabbit sera. these antisera did not inhibit asst activity. asst was recognized by the igg fraction of the antisera, but rat liver phenol sulfotransferase did not show cross-reactivity to asst on western blot (immunoblot) analysis. the asst was purified by an anti-as ... | 1994 | 7982888 |
| [scanning electron microscopic studies of the urinary bladder of sows with special reference to a eubacterium suis infection]. | scanning electron microscopy was performed on bladder biopsies from 20 sows with cystitis. results were compared with those of healthy control animals. biopsy specimens were taken from nine sows infected only with commensal bacteria (e. coli, streptococci, staphylococci) and from 11 sows, where eubacterium suis (e. suis) was involved in urinary tract infection and had caused a haemorrhagic cystitis. increased losses of normal superficial cells covered with microplicae could be seen depending on ... | 1994 | 7985429 |
| molecular cloning and sequence analysis of the proba operon from an extremely thermophilic eubacterium thermus thermophilus. | a 3.6 kb dna fragment carrying the thermus thermophilus proba region, which encodes the first two steps in the proline biosynthetic pathway, was cloned from the thermus thermophilus gene library, and its complete nucleotide sequence was determined. the deduced amino acid sequence of gamma-glutamyl kinase (40,657 da), the product of prob gene, and gamma-glutamyl phosphate reductase (48,747 da), the product of proa gene, showed 44.1% and 44.4% identity to those of escherichia coli, respectively. t ... | 1994 | 7988899 |
| relationship between clinical symptoms and enzyme-producing bacteria isolated from infected root canals. | the object of this study was to determine the correlation between clinical symptoms and the activity of enzymes such as collagenase, chondroitinase, and hyaluronidase produced by bacteria isolated from infected root canals. the materials examined consisted of 28 teeth with apical periodontitis from 25 patients. bacteria producing collagenase or chondroitinase and hyaluronidase were found to be significantly related to subacute clinical symptoms involving percussion pain. the frequency of bacteri ... | 1994 | 8006569 |
| [bacteria of the genus eubacterium and their participation in steroid metabolism]. | | 1994 | 8017116 |