| heterologous expression of a recombinant lactobacillal β-galactosidase in lactobacillus plantarum: effect of different parameters on the sakacin p-based expression system. | two overlapping genes lacl and lacm (laclm) encoding for heterodimeric β-galactosidase from lactobacillus reuteri were previously cloned and over-expressed in the food-grade host strain lactobacillus plantarum wcfs1, using the inducible lactobacillal psip expression system. in this study, we analyzed different factors that affect the production of recombinant l. reuteri β-galactosidase. | 2015 | 25880197 |
| gut dysbiosis and detection of "live gut bacteria" in blood of japanese patients with type 2 diabetes. | mounting evidence indicates that the gut microbiota are an important modifier of obesity and diabetes. however, so far there is no information on gut microbiota and "live gut bacteria" in the systemic circulation of japanese patients with type 2 diabetes. | 2014 | 24824547 |
| experimental evaluation of host adaptation of lactobacillus reuteri to different vertebrate species. | the species lactobacillus reuteri has diversified into host-specific lineages implying a long term association with different vertebrates. strains from rodent lineages show specific adaptations to mice, but the processes underlying the evolution of l. reuteri in other hosts remain unknown. we administered three standardized inocula composed of strains from different host-confined lineages to mice, pigs, chickens and humans. the ecological performance of each strain in the gastrointestinal tract ... | 2017 | 28389535 |
| a flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic lactobacillus reuteri. | eosinophils are resident leukocytes of gut mucosa. here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, gram-positive lactobacillus reuteri, in studies performed ex vivo. the assay may be adapted for use in vivo. | 2017 | 28359737 |
| molecular and biopharmaceutical investigation of alginate-inulin synbiotic co-encapsulation of probiotic to target the colon. | colon targeting, as a site-specific delivery for oral formulation, remains a major challenge, especially for sensitive bioactive components such as therapeutic forms of phages, live attenuated virus, and prebiotics-probiotics association. synbiotics could be used to protect encapsulated probiotics during the gastrointestinal tract and control their release in the colon. to achieve these goals, effective prebiotics such as inulin could be combined with alginate-the most exploited polymer used for ... | 2017 | 28350268 |
| probiotic lactobacillus reuteri has antifungal effects on oral candida species in vitro. | background: an alternative approach for managing candida infections in the oral cavity by modulating the oral microbiota with probiotic bacteria has been proposed. objective: the aim was to investigate the antifungal potential of the probiotic bacterium lactobacillus reuteri (dsm 17938 and atcc pta 5289) against six oral candida species (c. albicans, c. glabrata, c. krusei, c. tropicalis, c. dubliniensis, and c. parapsilosis). design: the lactobacilli were tested for their ability to co-aggregat ... | 2017 | 28326154 |
| lactobacillus reuteri surface mucus adhesins upregulate inflammatory responses through interactions with innate c-type lectin receptors. | the vertebrate gut symbiont lactobacillus reuteri exhibits strain-specific adhesion and health-promoting properties. here, we investigated the role of the mucus adhesins, cmba and mub, upon interaction of l. reuteri atcc pta 6475 and atcc 53608 strains with human monocyte-derived dendritic cells (modcs). we showed that mucus adhesins increased the capacity of l. reuteri strains to interact with modcs and promoted phagocytosis. our data also indicated that mucus adhesins mediate anti- and pro-inf ... | 2017 | 28326063 |
| correction: identification and characterization of sulfated carbohydrate-binding protein from lactobacillus reuteri. | [this corrects the article doi: 10.1371/journal.pone.0083703.]. | 2017 | 28291819 |
| microbiome and metabolome modifying effects of several cardiovascular disease interventions in apo-e(-/-) mice. | there is strong evidence indicating that gut microbiota have the potential to modify, or be modified by the drugs and nutritional interventions that we rely upon. this study aims to characterize the compositional and functional effects of several nutritional, neutraceutical, and pharmaceutical cardiovascular disease interventions on the gut microbiome, through metagenomic and metabolomic approaches. apolipoprotein-e-deficient mice were fed for 24 weeks either high-fat/cholesterol diet alone (con ... | 2017 | 28285599 |
| draft genome sequence of lactobacillus reuteri 121, a source of α-glucan and β-fructan exopolysaccharides. | the probiotic bacterium lactobacillus reuteri 121 is a well-known producer of diverse homoexopolysaccharides (α-glucans and β-fructans) from sucrose and maltodextrins/starches of interest for food applications. here, we report the draft genome sequence of this strain, with a focus on carbohydrate-active enzymes. | 2017 | 28280024 |
| catechol glucosides act as donor/acceptor substrates of glucansucrase enzymes of lactobacillus reuteri. | previously, we have shown that the glucansucrase gtfa-δn enzyme of lactobacillus reuteri 121, incubated with sucrose, efficiently glucosylated catechol and we structurally characterized catechol glucosides with up to five glucosyl units attached (te poele et al. in bioconjug chem 27:937-946, 2016). in the present study, we observed that upon prolonged incubation of gtfa-δn with 50 mm catechol and 1000 mm sucrose, all catechol had become completely glucosylated and then started to reappear. follo ... | 2017 | 28258313 |
| structural analysis of rebaudioside a derivatives obtained by lactobacillus reuteri 180 glucansucrase-catalyzed trans-α-glucosylation. | the wild-type gtf180-δn glucansucrase enzyme from lactobacillus reuteri 180 was found to catalyze the α-glucosylation of the steviol glycoside rebaudioside a, using sucrose as glucosyl donor in a transglucosylation process. structural analysis of the formed products by maldi-tof mass spectrometry, methylation analysis and nmr spectroscopy showed that rebaudioside a is specifically α-d-glucosylated at the steviol c-19 β-d-glucosyl moiety (55% conversion). the main product is a mono-(α1 → 6)-gluco ... | 2017 | 28231561 |
| erratum to: effect of lactobacillus reuteri on cell viability and pge2 production in human gingival fibroblasts. | | 2017 | 28210874 |
| efficacy and safety of apt198k for the treatment of infantile colic: a pilot study. | comparing efficacy and safety of apt198k (xyloglucan plus heat-killed lactobacillus reuteri sgl01 and bifidobacterium brevis sgb01) versus a lactase dietary supplement as first-line treatment of infantile colic. | 2017 | 28114795 |
| crystal structure of 4,6-α-glucanotransferase supports diet-driven evolution of gh70 enzymes from α-amylases in oral bacteria. | food processing and refining has dramatically changed the human diet, but little is known about whether this affected the evolution of enzymes in human microbiota. we present evidence that glycoside hydrolase family 70 (gh70) glucansucrases from lactobacilli, synthesizing α-glucan-type extracellular polysaccharides from sucrose, likely evolved from gh13 starch-acting α-amylases, via gh70 4,6-α-glucanotransferases. the crystal structure of a 4,6-α-glucanotransferase explains the mode of action an ... | 2017 | 28065507 |
| effect of biochanin a on corn grain (zea mays) fermentation by bovine rumen amylolytic bacteria. | the objective was to determine the effect of biochanin a (bca), an isoflavone produced by red clover (trifolium pratense l.), on corn fermentation by rumen micro-organisms. | 2017 | 28055130 |
| the level of beta defensin-2 in saliva and its expression in parotid gland epithelial cells after probiotic (lactobacillus reuteri) induction to inhibit streptococcus mutans in caries. | the aim of this study was to prove that administrating l. reuteri probiotics can increase the level of bd-2 saliva and bd-2 expression in the epithelial parotid glands of wistar rats. | 2017 | 28042275 |
| characterisations of lactobacillus reuteri sk24.003 glucansucrase: implications for α-gluco-poly- and oligosaccharides biosynthesis. | glucansucrase was obtained from lactobacillus reuteri sk24.003 and its characterizations and in vitro biosynthesis for glucose polymer and oligosaccharides were investigated. the final specific activity of glucansucrase was 1.3iu/mg protein with 8.6-fold purification and 8.7% recovery. the molecular weight of purified enzyme was 166.0kda. the glucansucrase exhibited optimum activity at 30-35°c, whereas the maximum activity was obtained at ph 5.0-5.5. the double-charged ions including mg(2+), mn( ... | 2017 | 28041553 |
| redox balance in lactobacillus reuteri dsm20016: roles of iron-dependent alcohol dehydrogenases in glucose/ glycerol metabolism. | lactobacillus reuteri, a heterofermentative bacterium, metabolizes glycerol via a pdu (propanediol-utilization) pathway involving dehydration to 3-hydroxypropionaldehyde (3-hpa) followed by reduction to 1,3-propandiol (1,3-pdo) with concomitant generation of an oxidized cofactor, nad+ that is utilized to maintain cofactor balance required for glucose metabolism and even for oxidation of 3-hpa by a pdu oxidative branch to 3-hydroxypropionic acid (3-hp). the pdu pathway is operative inside pdu mic ... | 2016 | 28030590 |
| effect of lactobacillus reuteri on cell viability and pge2 production in human gingival fibroblasts. | emerging evidence suggests that probiotic therapy can play a role in the prevention and management of oral inflammatory diseases through immunomodulation and down-regulation of the inflammatory cascade. the aim of this in vitro study was to investigate the viability of human gingival fibroblasts (hgf) and its production of prostaglandin e2 (pge2), when exposed to supernatants of two mixed lactobacillus reuteri strains (atcc pta 5289 and dsm 17938). the experiments were conducted in the presence ... | 2016 | 28028690 |
| metabolic effects of lactobacillus reuteri dsm 17938 in people with type 2 diabetes: a randomized controlled trial. | to investigate the metabolic effects of 12-week oral supplementation with lactobacillus reuteri dsm 17938 in patients with type 2 diabetes on insulin therapy. | 2017 | 28009106 |
| effects of probiotic drop containing lactobacillus rhamnosus, bifidobacterium infantis, and lactobacillus reuteri on salivary streptococcus mutans and lactobacillus levels. | the aim of the present study was to evaluate the effect of a probiotic drop containing lactobacillus rhamnosus, bifidobacterium infantis, and lactobacillus reuteri on salivary counts of streptococcus mutans (sm) and lactobacillus (lb) in children 3-6 years of age. | 2017 | 27994413 |
| resetting microbiota by lactobacillus reuteri inhibits t reg deficiency-induced autoimmunity via adenosine a2a receptors. | regulatory t (t reg) cell deficiency causes lethal, cd4(+) t cell-driven autoimmune diseases. stem cell transplantation is used to treat these diseases, but this procedure is limited by the availability of a suitable donor. the intestinal microbiota drives host immune homeostasis by regulating the differentiation and expansion of t reg, th1, and th2 cells. it is currently unclear if t reg cell deficiency-mediated autoimmune disorders can be treated by targeting the enteric microbiota. here, we d ... | 2017 | 27994068 |
| potential of lactobacillus reuteri from spontaneous
sourdough as a starter additive for improving quality parameters of bread. | retardation of microbial spoilage of bread can be achieved by the use of spontaneous sourdough with an antimicrobial activity. this study was undertaken to identify lactic acid bacteria naturally occurring in spontaneous sourdough and use them for quality improvement and prolonging shelf life of rye, wheat and rye with wheat bread. identification of isolates from spontaneous sourdough by pyrosequencing assay showed that lactobacillus reuteri were dominant lactic acid bacteria. the isolates showe ... | 2016 | 27956866 |
| expression of an (engineered) 4,6-α-glucanotransferase in potato results in changes in starch characteristics. | starch structure strongly influences starch physicochemical properties, determining the end uses of starch in various applications. to produce starches with novel structure and exploit the mechanism of starch granule formation, an (engineered) 4, 6-α-glucanotransferase (gtfb) from lactobacillus reuteri 121 was introduced into two potato genetic backgrounds: amylose-containing line kardal and amylose-free mutant amf. the resulting starches showed severe changes in granule morphology regardless of ... | 2016 | 27911907 |
| lactobacillus reuteri dsm 17938 in the treatment of functional abdominal pain in children - rct study. | beneficial therapeutic effect of probiotics has been reported in children with the irritable bowel syndrome (ibs) but not consistently in other functional abdominal pain-related disorders. the aim of this study was to investigate the effect of lactobacillus (l.) reuteri dsm 17938 in the treatment of functional abdominal pain (fap) and ibs in children. | 2016 | 27906800 |
| asymmetric chemoenzymatic synthesis of 1,3-diols and 2,4-disubstituted aryloxetanes by using whole cell biocatalysts. | regio- and stereo-selective reduction of substituted 1,3-aryldiketones, investigated in the presence of different whole cell microorganisms, was found to afford β-hydroxyketones or 1,3-diols in very good yields (up to 95%) and enantiomeric excesses (up to 96%). the enantiomerically enriched aldols, obtained with the opposite stereo-preference by baker's yeast and lactobacillus reuteri dsm 20016 bioreduction, could then be diastereoselectively transformed into optically active syn- or anti-1,3-di ... | 2016 | 27878162 |
| presence of lactobacillus reuteri in saliva coincide with higher salivary iga in young adults after intake of probiotic lozenges. | the aim of this study was to compare the concentration of salivary immunoglobulin a (iga) and the selected interleukins (il)-1β, il-6, il-8 and il-10 in young individuals with presence and non-presence of lactobacillus reuteri in saliva after a three-week intervention with probiotic lozenges. the study group consisted of 47 healthy individuals aged 18-32 years with no clinical signs of oral inflammation. in a randomised, double-blind, placebo-controlled, cross-over trial participants ingested tw ... | 2017 | 27873545 |
| crosslinked, cryostructured lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol. | crosslinked, cryostructured monoliths prepared from lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3hpa), 3-hydroxypropionic acid (3hp) and 1,3-propanediol (1,3pdo). glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (pei/pva) were evaluated as crosslinkers; the latter gave highly stable prepa ... | 2017 | 27829124 |
| microbial lysate upregulates host oxytocin. | neuropeptide hormone oxytocin has roles in social bonding, energy metabolism, and wound healing contributing to good physical, mental and social health. it was previously shown that feeding of a human commensal microbe lactobacillus reuteri (l. reuteri) is sufficient to up-regulate endogenous oxytocin levels and improve wound healing capacity in mice. here we show that oral l. reuteri-induced skin wound repair benefits extend to human subjects. further, dietary supplementation with a sterile lys ... | 2017 | 27825953 |
| draft genome sequences of lactobacillus animalis strain p38 and lactobacillus reuteri strain p43 isolated from chicken cecum. | here, we present the genome sequence of lactobacillus animalis strain p38 and lactobacillus reuteri strain p43, both isolated from the cecum content of a 4-week old chicken fed a diet supplemented with the prebiotic β(1-4)galacto-oligosaccharide (gos). these indigenous lactobacillus isolates are potential probiotic organisms for poultry. | 2016 | 27811108 |
| microbes and oxytocin: benefits for host physiology and behavior. | it is now understood that gut bacteria exert effects beyond the local boundaries of the gastrointestinal tract to include distant tissues and overall health. prototype probiotic bacterium lactobacillus reuteri has been found to upregulate hormone oxytocin and systemic immune responses to achieve a wide array of health benefits involving wound healing, mental health, metabolism, and myoskeletal maintenance. together these display that the gut microbiome and host animal interact via immune-endocri ... | 2016 | 27793228 |
| changes in the diversity and composition of gut microbiota of weaned piglets after oral administration of lactobacillus or an antibiotic. | the gut microbiota plays important roles in the health and well-being of animals, and high-throughput sequencing facilitates exploration of microbial populations in the animal gut. however, previous studies have focused on fecal samples instead of the gastrointestinal tract. in this study, we compared the microbiota diversity and composition of intestinal contents of weaned piglets treated with lactobacillus reuteri or chlortetracycline (aureomycin) using high-throughput sequencing. nine weaned ... | 2016 | 27757509 |
| structural determinants of alternating (α1 → 4) and (α1 → 6) linkage specificity in reuteransucrase of lactobacillus reuteri. | the glucansucrase gtfa of lactobacillus reuteri 121 produces an α-glucan (reuteran) with a large amount of alternating (α1 → 4) and (α1 → 6) linkages. the mechanism of alternating linkage formation by this reuteransucrase has remained unclear. gtfo of the probiotic bacterium lactobacillus reuteri atcc 55730 shows a high sequence similarity (80%) with gtfa of l. reuteri 121; it also synthesizes an α-glucan with (α1 → 4) and (α1 → 6) linkages, but with a clearly different ratio compared to gtfa. i ... | 2016 | 27748434 |
| lactobacillus reuteri dsm 17938 plus vitamin d3 as ancillary treatment in allergic children with asthma. | | 2016 | 27720582 |
| synthesis of taste-active γ-glutamyl dipeptides during sourdough fermentation by lactobacillus reuteri. | this study aimed to assess whether peptides influence the taste of sourdough bread. γ-glutamyl dipeptides with known kokumi taste threshold, namely γ-glu-glu, γ-glu-leu, γ-glu-ile, γ-glu-phe, γ-glu-met, and γ-glu-val, were identified in sourdough by liquid chromatography-tandem mass spectrometry in mrm mode. γ-glutamyl dipeptides were found in higher concentrations in sourdough fermented with lactobacillus reuteri when compared to the chemically acidified controls. proteolysis was an important f ... | 2016 | 27641253 |
| lactobacillus reuteri and staphylococcus aureus differentially influence the generation of monocyte-derived dendritic cells and subsequent autologous t cell responses. | in early-life, the immature mucosal barrier allows contact between the gut microbiota and the developing immune system. due to their strategic location and their ability to sample luminal antigen, dendritic cells (dc) play a central role in the interaction of microbes and immune cells in the gut. here, we investigated how two bacteria associated with opposite immune profiles in children, that is, lactobacillus (l.) reuteri and staphylococcus (s.) aureus, influenced the differentiation of monocyt ... | 2016 | 27621814 |
| probiotic administration among free-living older adults: a double blinded, randomized, placebo-controlled clinical trial. | diseases of the digestive system have been found to contribute to a higher symptom burden in older adults. thus, therapeutic strategies able to treat gastrointestinal discomfort might impact the overall health status and help older adults to increase their overall health status and optimal functionality. | 2016 | 27612653 |
| the role of probiotics in prevention of oral diseases. | the dynamic development of knowledge in the field of probiotics was commenced at the beginning of the 20th century. since then, many ways of their possible usage in medicine have been established. in accordance with the who, probiotics are live microorganisms, which if applied in adequate amounts may benefit the host. among probiotics, fungi and bacteria are distinguished, and mechanisms of action of these organisms in the oral cavity and gut are parallel. application in dentistry, in prophylaxi ... | 2016 | 27594560 |
| oral lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 to reduce group b streptococcus colonization in pregnant women: a randomized controlled trial. | this study is to examine the effect of lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 taken orally before bedtime on group b streptococcus (gbs)-positive pregnant women with respect to becoming gbs negative. | 2016 | 27590374 |
| draft genome sequence of lactobacillus reuteri strain crl 1098, an interesting candidate for functional food development. | we report here the draft genome sequence of lactobacillus reuteri strain crl 1098. this strain represents an interesting candidate for functional food development because of its proven probiotic properties. the draft genome sequence is composed of 1,969,471 bp assembled into 45 contigs and an average g+c content of 38.8%. | 2016 | 27563038 |
| site directed mutagenesis of dextransucrase dsrm from weissella cibaria: transformation to a reuteransucrase. | glucansucrases produce α-glucans and gluco-oligosaccharides; the linkage type and molecular weight of glucans impacts their functionality. this study compared the catalytic specificities of dextransucrase dsrm from weissella cibaria 10m and derivatives of this enzymes with gtfa from lactobacillus reuteri tmw1.656. the n-variable region, which is dispensable for gtfa activity, was essential for dsrm activity. parallel amino acid substitutions in dsrm-δs and gtfa-δn indicated that the acceptor bin ... | 2016 | 27550198 |
| heat-killed lactobacillus reuteri gmnl-263 prevents epididymal fat accumulation and cardiac injury in high-calorie diet-fed rats. | high-calorie diet-induced obesity leads to cardiomyocyte dysfunction and apoptosis. impaired regulation of epididymal fat content in obese patients has been known to increase the risk of cardiac injury. in our study, a lactic acid bacteria, lactobacillus reuteri gmnl-263, was evaluated for its potential to reduce body weight and body fat ratio and to prevent heart injury in rats with high-fat diet-induced obesity. lactic acid bacteria supplementation restored the cardiac function and decreased t ... | 2016 | 27499689 |
| structural basis for the roles of starch and sucrose in homo-exopolysaccharide formation by lactobacillus reuteri 35-5. | lactic acid bacteria (lab) produce exopolysaccharides (eps) that are important for biofilm formation in the mammalian oral cavity and gastrointestinal tract. sucrose is a well-known substrate for homo-eps formation by lactobacillus reuteri glucansucrases (gs). starch is the main fermentable carbohydrate in the human diet, and often consumed simultaneously with sucrose. recently we have characterized l. reuteri strains that also possess 4,6-α-glucanotransferases (4,6-α-gtases) that act on starch ... | 2016 | 27474540 |
| probiotic lactobacilli modulate staphylococcus aureus-induced activation of conventional and unconventional t cells and nk cells. | lactobacilli are probiotic commensal bacteria and potent modulators of immunity. when present in the gut or supplemented as probiotics, they beneficially modulate ex vivo immune responsiveness. further, factors derived from several lactobacilli strains act immune regulatory in vitro. in contrast, staphylococcus aureus (s. aureus) is known to induce excessive t cell activation. in this study, we aimed to investigate s. aureus-induced activation of human mucosal-associated invariant t cells (mait ... | 2016 | 27462316 |
| regular consumption of lactobacillus reuteri-containing lozenges reduces pregnancy gingivitis: an rct. | this randomized controlled trial assessed the impact of lactobacillus reuteri on pregnancy gingivitis in healthy women. | 2016 | 27461133 |
| resonance raman spectroscopic study of the interaction between co(ii)rrinoids and the atp:corrinoid adenosyltransferase pduo from lactobacillus reuteri. | the human-type atp:corrinoid adenosyltransferase pduo from lactobacillus reuteri (lrpduo) catalyzes the adenosylation of co(ii)rrinoids to generate adenosylcobalamin (adocbl) or adenosylcobinamide (adocbi(+)). this process requires the formation of "supernucleophilic" co(i)rrinoid intermediates in the enzyme active site which are properly positioned to abstract the adeonsyl moiety from co-substrate atp. previous magnetic circular dichroism (mcd) spectroscopic and x-ray crystallographic analyses ... | 2016 | 27383231 |
| probiotics for irritable bowel syndrome: clinical data in children. | the purpose of this review was to summarize the evidence regarding probiotics treatment for pediatric ibs. | 2016 | 27380595 |
| beneficial effect of synbiotic supplementation on hepatic steatosis and anthropometric parameters, but not on gut permeability in a population with nonalcoholic steatohepatitis. | nonalcoholic fatty liver disease is the most prevalent chronic liver disease in western countries; it can progress to nonalcoholic steatohepatitis (nash), cirrhosis and hepatocarcinoma. the importance of gut-liver-adipose tissue axis has become evident and treatments targeting gut microbiota may improve inflammatory and metabolic parameters in nash patients. in a randomized, controlled clinical trial, involving 50 biopsy-proven nash patients, we investigated the effects of synbiotic supplementat ... | 2016 | 27367724 |
| folc2-mediated folate metabolism contributes to suppression of inflammation by probiotic lactobacillus reuteri. | bacterial-derived compounds from the intestinal microbiome modulate host mucosal immunity. identification and mechanistic studies of these compounds provide insights into host-microbial mutualism. specific lactobacillus reuteri strains suppress production of the proinflammatory cytokine, tumor necrosis factor (tnf), and are protective in a mouse model of colitis. human-derived l. reuteri strain atcc pta 6475 suppresses intestinal inflammation and produces 5,10-methenyltetrahydrofolic acid polygl ... | 2016 | 27353144 |
| doses lactobacillus reuteri depend on adhesive ability to modulate the intestinal immune response and metabolism in mice challenged with lipopolysaccharide. | the objective of this study was to evaluate the modulatory effects of lactobacillus reuteri zj617 and zj615, which have high and low adhesive abilities, respectively, and lactobacillus rhamnosus gg (lgg) on immune responses and metabolism in mice stimulated with lipopolysaccharide (lps). six c57bl/6 mice per group were orally inoculated with zj617, zj615 or lgg for one week (1 × 10(8) cfu/mouse) and i.p. injected with lps (10 mg/kg) for 24 h. compared with the lps stimulation group, zj615, zj617 ... | 2016 | 27323686 |
| effect of reuterin-producing lactobacillus reuteri coupled with glycerol on the volatile fraction, odour and aroma of semi-hard ewe milk cheese. | the effect of the biopreservation system formed by lactobacillus reuteri inia p572, a reuterin-producing strain, and glycerol (required for reuterin production), on the volatile fraction, aroma and odour of industrial sized semi-hard ewe milk cheese (castellano type) was investigated over a 3-month ripening period. the volatile compounds were extracted and analyzed by spme-gc-ms and cheese odour and aroma profiles were studied by descriptive sensory analysis. control cheese was made only with a ... | 2016 | 27289193 |
| lactobacillus reuteri treatment and dss colitis: new insight into the mechanism of protection. | | 2016 | 27228414 |
| heat-killed and live lactobacillus reuteri gmnl-263 exhibit similar effects on improving metabolic functions in high-fat diet-induced obese rats. | our objective was to investigate and compare the effects of heat-killed (hk) and live lactobacillus reuteri gmnl-263 (lr263) on insulin resistance and its related complications in high-fat diet (hfd)-induced rats. male sprague-dawley rats were fed with a hfd with either hk or live lr263 for 12 weeks. the increases in the weight gain, serum glucose, insulin, and lipid profiles in the serum and liver observed in the hfd group were significantly reduced after hk or live lr263 administration. feedin ... | 2016 | 27163114 |
| lactobacillus reuteri dsm 17938 for the management of functional abdominal pain in childhood: a randomized, double-blind, placebo-controlled trial. | to determine whether administration of lactobacillus reuteri dsm 17938 is beneficial in functional abdominal pain (fap) of childhood. | 2016 | 27156182 |
| biofilms of vaginal lactobacillus reuteri crl 1324 and lactobacillus rhamnosus crl 1332: kinetics of formation and matrix characterization. | adhesion and biofilm formation are strain properties that reportedly contribute to the permanence of lactobacilli in the human vagina. the kinetics of biofilm formation and the chemical nature of the biofilm matrix formed by lactobacillus reuteri crl (centro de referencia para lactobacilos culture collection) 1324 and lactobacillus rhamnosus crl 1332, vaginal beneficial strains, were evaluated in this work. crystal violet-stained microplate assay and techniques of epifluorescence, electron and c ... | 2016 | 27146055 |
| [non-antibiotic strategies to prevent the recurrence of uncomplicated urinary tract infections in women]. | the aim of all medical treatment is "primum nihil nocere" ("first, do no harm").restoring the integrity of intestinal microbiota and optimising the immune response in recurrent infections, especially in the urinary tract, are treatment alternatives which are closer to this target than the usual focus on antibiotic prevention of recurrence.in the future, antibiotics will continue to be recommended for the prevention of urinary tract infections on a case-by-case basis. however, the problems of an ... | 2016 | 27138235 |
| complete genome sequence of probiotic lactobacillus reuteri zlr003 isolated from healthy weaned pig. | lactobacillus reuteri zlr003 was isolated from the caecum mucosa of healthy weaned pigs with displaying probiotic properties in our laboratory. here, we present the complete genome sequence of l. reuteri zlr003, which consists of a circular 2,234,097bp chromosome (g+c content of 38.66%). such information will provide insights into the molecular mechanism of its probiotic activity and facilitate its application in animal production. | 2016 | 27130498 |
| lactobacillus reuteri supplements do not affect salivary iga or cytokine levels in healthy subjects: a randomized, double-blind, placebo-controlled, cross-over trial. | to evaluate the effect of daily ingestion of probiotic lactobacilli on the levels of secretory iga (siga) and selected cytokines in whole saliva of healthy young adults. | 2016 | 27104984 |
| lactobacillus reuteri increases mucus thickness and ameliorates dextran sulphate sodium-induced colitis in mice. | the aim of this study was to investigate whether two lactobacillus reuteri strains (rat-derived r2lc and human-derived atcc pta 4659 (4659)) could protect mice against colitis, as well as delineate the mechanisms behind this protection. | 2016 | 27096537 |
| characterization of the anti-inflammatory lactobacillus reuteri bm36301 and its probiotic benefits on aged mice. | the gut microbiota is playing more important roles in host immune regulation than was initially expected. since many benefits of microbes are highly strain-specific and their mechanistic details remain largely elusive, further identification of new probiotic bacteria with immunoregulatory potentials is of great interest. | 2016 | 27095067 |
| letter to the editor on 'enhancing vitamin b12 content in soy-yogurt by lactobacillus reuteri, ijfm. 206:56-59'. | | 2016 | 27085811 |
| probiotics [lgg-bb12 or rc14-gr1] versus placebo as prophylaxis for urinary tract infection in persons with spinal cord injury [prosciuttu]: a study protocol for a randomised controlled trial. | urinary tract infections [utis] are very common in people with spinal cord injury [sci]. utis are increasingly difficult and expensive to treat as the organisms that cause them become more antibiotic resistant. among the sci population, there is a high rate of multi-resistant organism [mro] colonisation. non-antibiotic prevention strategies are needed to prevent uti without increasing resistance. probiotics have been reported to be beneficial in preventing utis in post-menopausal women in severa ... | 2016 | 27084704 |
| lactobacilli require physical contact to reduce staphylococcal tsst-1 secretion and vaginal epithelial inflammatory response. | italic! staphylococcus aureusbiofilms can be found on vaginal epithelia, secreting toxins and causing inflammation. the co-vaginal species italic! lactobacilluscan alter staphylococcal-induced epithelial secretion of inflammatory cytokines and quench staphylococcal toxic shock syndrome toxin-1 secretion. it is hypothesized that these effects of lactobacilli require direct physical contact between lactobacilli, staphylococci and the epithelium. indeed, lactobacilli only reduced italic! s. aureus- ... | 2016 | 27060097 |
| lactobacillus reuteri 6475 increases bone density in intact females only under an inflammatory setting. | we previously demonstrated that short-term oral administration of the probiotic lactobacillus reuteri 6475 enhanced bone density in male but not female mice. we also established that l. reuteri 6475 enhanced bone health and prevented bone loss in estrogen-deficient female mice. in this study, we tested whether a mild inflammatory state and/or a long-term treatment with the probiotic was required to promote a positive bone effect in estrogen-sufficient female mice. | 2016 | 27058036 |
| glucansucrase gtf180-δn of lactobacillus reuteri 180: enzyme and reaction engineering for improved glycosylation of non-carbohydrate molecules. | glucansucrases have a broad acceptor substrate specificity and receive increased attention as biocatalysts for the glycosylation of small non-carbohydrate molecules using sucrose as donor substrate. however, the main glucansucrase-catalyzed reaction results in synthesis of α-glucan polysaccharides from sucrose, and this strongly impedes the efficient glycosylation of non-carbohydrate molecules and complicates downstream processing of glucosylated products. this paper reports that suppressing α-g ... | 2016 | 27052379 |
| intestinal removal of free fatty acids from hosts by lactobacilli for the treatment of obesity. | recent findings on the association of gut microbiota with various diseases, including obesity, prompted us to investigate the possibility of using a certain type of gut bacteria as a safe therapeutic for obesity. lactobacillus mutants with enhanced capacity in absorption of free fatty acids (ffas) were isolated to show reduced absorption of ffas by the administered host, attributing to inhibition of body weight gain and body fat accumulation as well as amelioration of blood profiles. consequentl ... | 2016 | 27047743 |
| impact of oral probiotics on neurodevelopmental outcomes in preterm infants. | the aim of the study was to evaluate the neurodevelopment outcomes of very low birth weight (vlbw) preterm infants supplemented with oral probiotics for the prevention of necrotizing enterocolitis (nec). | 2017 | 27045204 |
| effect of formula containing lactobacillus reuteri dsm 17938 on fecal microbiota of infants born by cesarean-section. | microbiota modulation by probiotics in infants born by cesarean (c)-section is poorly understood. we aimed at assessing the response of c-section-delivered infant microbiota to a formula containing lactobacillus reuteri deutsche sammlung von mikroorganismen und zellkulturen gmbh (dsm) 17938 and comparing it with that of vaginally delivered infants. | 2016 | 27035371 |
| harvesting the benefits of biofilms: a novel probiotic delivery system for the prevention of necrotizing enterocolitis. | probiotics reduce the incidence of necrotizing enterocolitis (nec) albeit only when administered at high frequency (at least daily). we have developed a novel probiotic delivery system in which probiotics are grown as a biofilm on microspheres, allowing enhanced efficacy with only a single treatment. | 2016 | 27032609 |
| isolation of lactobacillus reuteri from peyer's patches and their effects on siga production and gut microbiota diversity. | we previously reported that specific lactobacillus reuteri colonized within mouse peyer's patches (pp) effectively prevented high fat diet induced obesity and low-grade chronic inflammation. we further investigated the role of pp lactobacillus reuteri on siga production in rats in this study. | 2016 | 27030202 |
| postbiotic modulation of retinoic acid imprinted mucosal-like dendritic cells by probiotic lactobacillus reuteri 17938 in vitro. | lactobacilli are widely used as probiotics with beneficial effects on infection-associated diarrhea, but also used in clinical trials of e.g., necrotizing enterocolitis and inflammatory bowel diseases. the possibility of using probiotic metabolic products, so-called postbiotics, is desirable as it could prevent possible side effects of live bacteria in individuals with a disturbed gut epithelial barrier. here, we studied how lactobacillus reuteri dsm 17938 cell-free supernatant (l. reuteri-cfs) ... | 2016 | 27014275 |
| in vivo and in vitro detection of luminescent and fluorescent lactobacillus reuteri and application of red fluorescent mcherry for assessing plasmid persistence. | lactobacillus reuteri is a symbiont that inhabits the gastrointestinal (gi) tract of mammals, and several strains are used as probiotics. after introduction of probiotic strains in a complex ecosystem like the gi tract, keeping track of them is a challenge. the main objectives of this study were to introduce reporter proteins that would enable in vivo and in vitro detection of l. reuteri and increase knowledge about its interactions with the host. we describe for the first time cloning of codon- ... | 2016 | 27002525 |
| lactobacillus reuteri strains convert starch and maltodextrins into homoexopolysaccharides using an extracellular and cell-associated 4,6-α-glucanotransferase. | exopolysaccharides (eps) of lactic acid bacteria (lab) are of interest for food applications. lab are well-known to produce α-glucan from sucrose by extracellular glucansucrases. various lactobacillus reuteri strains also possess 4,6-α-glucanotransferase (4,6-α-gtase) enzymes. purified 4,6-α-gtases (e.g., gtfb) were shown to act on starches (hydrolysates), cleaving α1→4 linkages and synthesizing α1→6 linkages, yielding isomalto-/maltopolysaccharides (immp). here we report that also l. reuteri ce ... | 2016 | 26996545 |
| systematic review with meta-analysis: lactobacillus reuteri dsm 17938 for diarrhoeal diseases in children. | not all probiotics are equal. | 2016 | 26991503 |
| probiotics for the treatment of infantile colic: a systematic review. | to evaluate whether clinical data support the safety and efficacy of probiotics for the management of infantile colic. | 2016 | 26940647 |
| beneficial bacteria inhibit cachexia. | muscle wasting, known as cachexia, is a debilitating condition associated with chronic inflammation such as during cancer. beneficial microbes have been shown to optimize systemic inflammatory tone during good health; however, interactions between microbes and host immunity in the context of cachexia are incompletely understood. here we use mouse models to test roles for bacteria in muscle wasting syndromes. we find that feeding of a human commensal microbe, lactobacillus reuteri, to mice is suf ... | 2016 | 26933816 |
| immortalized mh-s cells lack defining features of primary alveolar macrophages and do not support mouse pneumovirus replication. | the sv-40-transformed mh-s cell line maintains some, but not all, features of primary alveolar macrophages (ams) from balb/c mice. we show here that mh-s cells produce inflammatory cytokines il-6 and cxcl10 in response to challenge with gram-positive lactobacillus reuteri, and to tlr2 and nod2 ligands pam3csk4 and mdp, respectively. in contrast, although wild-type ams are infected in vivo by pneumonia virus of mice (pvm), no virus replication was detected in mh-s cells. interestingly, the surfac ... | 2016 | 26916143 |
| glucosylation of catechol with the gtfa glucansucrase enzyme from lactobacillus reuteri and sucrose as donor substrate. | lactic acid bacteria use glucansucrase enzymes for synthesis of gluco-oligosaccharides and polysaccharides (α-glucans) from sucrose. depending on the glucansucrase enzyme, specific α-glucosidic linkages are introduced. gtfa-δn (n-terminally truncated glucosyltransferase a) is a glucansucrase enzyme of lactobacillus reuteri 121 that synthesizes the reuteran polysaccharide with (α1 → 4) and (α1 → 6) glycosidic linkages. glucansucrases also catalyze glucosylation of various alternative acceptor sub ... | 2016 | 26898769 |
| bacterial succession in the broiler gastrointestinal tract. | a feeding trial was performed with broilers receiving a diet of wheat-based feed (wbf), maize-based feed (mbf), or maize-based concentrates supplemented with 15% or 30% crimped kernel maize silage (ckms-15 or ckms-30, respectively). the aim of the study was to investigate the bacterial community compositions of the crop, gizzard, ileum, and cecum contents in relation to the feeding strategy and age (8, 15, 22, 25, 29, or 36 days). among the four dietary treatments, bacterial diversity was analyz ... | 2016 | 26873323 |
| the gram-negative bacterium azotobacter chroococcum ncimb 8003 employs a new glycoside hydrolase family 70 4,6-α-glucanotransferase enzyme (gtfd) to synthesize a reuteran like polymer from maltodextrins and starch. | originally the glycoside hydrolase (gh) family 70 only comprised glucansucrases of lactic acid bacteria which synthesize α-glucan polymers from sucrose. recently we have identified 2 novel subfamilies of gh70 enzymes represented by the lactobacillus reuteri 121 gtfb and the exiguobacterium sibiricum 255-15 gtfc enzymes. both enzymes catalyze the cleavage of (α1→4) linkages in maltodextrin/starch and the synthesis of consecutive (α1→6) linkages. here we describe a novel gh70 enzyme from the nitro ... | 2016 | 26868718 |
| structure modeling and functional analysis of recombinant dextransucrase from weissella confusa cab3 expressed in lactococcus lactis. | the dextransucrase gene from weissella confusa cab3, having an open reading frame of 4.2 kb coding for 1,402 amino acids, was amplified, cloned, and expressed in lactococcus lactis. the recombinant dextransucrase, wccab3-rdsr was expressed as extracellular enzyme in m17 medium with a specific activity of 1.5 u/mg which after purification by peg-400 fractionation gave 6.1 u/mg resulting in 4-fold purification. wccab3-rdsr was expressed as soluble and homogeneous protein of molecular mass, approxi ... | 2016 | 26861959 |
| a prebiotic matrix for encapsulation of probiotics: physicochemical and microbiological study. | this work aims to develop an encapsulated oral-synbiotic supplement by studying the effect of adding inulin in alginate beads and observing its ability to protect three probiotic strains: pediocucus acidilactici, lactobacillus reuteri and lactobacillus salivarius. beads of different inulin concentrations 0%, 5%, 10%, 15% and 20% (w/v) in 2% (w/v) alginate solution were prepared by the extrusion/ionotropic gelation method. polymer distribution within beads was characterised using confocal laser s ... | 2016 | 26805512 |
| influence of prebiotics on lactobacillus reuteri death kinetics under sub-optimal temperatures and ph. | eaten foodstuffs are usually fortified with prebiotic ingredients, such as inulin and oligofructose (fos). the main goal of this study was to evaluate the combined effects of inulin and fos with either suboptimal ph or storage temperature on the viability of lactobacillus reuteri dsm 20016. data were modeled through weibull equation for the evaluation of the microbiological shelf life and the survival time. prebiotics enhanced the microbiological shelf life and enhanced the survival time of the ... | 2016 | 26804428 |
| synthesis of new hyperbranched α-glucans from sucrose by lactobacillus reuteri 180 glucansucrase mutants. | α-glucans produced by glucansucrase enzymes of lactic acid bacteria attract strong attention as novel ingredients and functional biopolymers in the food industry. in the present study, α-helix 4 amino acid residues d1085, r1088, and n1089 of glucansucrase gtf180 of lactobacillus reuteri 180 were targeted for mutagenesis both jointly and separately. analysis of the mutational effects on enzyme function revealed that all d1085 and r1088 mutants catalyzed the synthesis of hyperbranched α-glucans wi ... | 2016 | 26688101 |
| histamine h2 receptor-mediated suppression of intestinal inflammation by probiotic lactobacillus reuteri. | probiotics and commensal intestinal microbes suppress mammalian cytokine production and intestinal inflammation in various experimental model systems. limited information exists regarding potential mechanisms of probiotic-mediated immunomodulation in vivo. in this report, we demonstrate that specific probiotic strains of lactobacillus reuteri suppress intestinal inflammation in a trinitrobenzene sulfonic acid (tnbs)-induced mouse colitis model. only strains that possess the hdc gene cluster, inc ... | 2015 | 26670383 |
| comparative genomics lactobacillus reuteri from sourdough reveals adaptation of an intestinal symbiont to food fermentations. | lactobacillus reuteri is a dominant member of intestinal microbiota of vertebrates, and occurs in food fermentations. the stable presence of l. reuteri in sourdough provides the opportunity to study the adaptation of vertebrate symbionts to an extra-intestinal habitat. this study evaluated this adaptation by comparative genomics of 16 strains of l. reuteri. a core genome phylogenetic tree grouped l. reuteri into 5 clusters corresponding to the host-adapted lineages. the topology of a gene conten ... | 2015 | 26658825 |
| infant colic-what works: a systematic review of interventions for breast-fed infants. | to determine the strength of evidence for commonly used interventions for colic in breast-fed and mixed-fed infants younger than 6 months. | 2016 | 26655941 |
| a randomized double-blind placebo-controlled trial of lactobacillus reuteri for chronic functional abdominal pain in children. | functional abdominal pain (fap) is one of the most common diseases, and large percentages of children suffer from it. | 2015 | 26635937 |
| the pan-genome of lactobacillus reuteri strains originating from the pig gastrointestinal tract. | lactobacillus reuteri is a gut symbiont of a wide variety of vertebrate species that has diversified into distinct phylogenetic clades which are to a large degree host-specific. previous work demonstrated host specificity in mice and begun to determine the mechanisms by which gut colonisation and host restriction is achieved. however, how l. reuteri strains colonise the gastrointestinal (gi) tract of pigs is unknown. | 2015 | 26626322 |
| high-fat-diet-induced obesity is associated with decreased antiinflammatory lactobacillus reuteri sensitive to oxidative stress in mouse peyer's patches. | diet-induced inflammation in the small intestine may represent an early event that precedes and predisposes to obesity and insulin resistance. this is related to decrease of lactobacilli in peyer's patches (pp) revealed in our previous study. the present study aimed to clarify specific changes of pp lactobacillus on the strain level and related biological activity. | 2016 | 26620713 |
| synbiotic approach restores intestinal homeostasis and prolongs survival in leukaemic mice with cachexia. | cancer cachexia is a multifactorial syndrome that includes muscle wasting and inflammation. as gut microbes influence host immunity and metabolism, we investigated the role of the gut microbiota in the therapeutic management of cancer and associated cachexia. a community-wide analysis of the caecal microbiome in two mouse models of cancer cachexia (acute leukaemia or subcutaneous transplantation of colon cancer cells) identified common microbial signatures, including decreased lactobacillus spp. ... | 2016 | 26613342 |
| colic in infants. | colic in infants leads one in six families (17%) with children to consult a health professional. one systematic review of 15 community-based studies found a wide variation in prevalence, which depended on study design and method of recording. | 2015 | 26581647 |
| effectiveness of lactobacillus reuteri dsm 17938 for the prevention of nosocomial diarrhea in children: a randomized, double-blind, placebo-controlled trial. | multiple studies of probiotics used to prevent nosocomial diarrhea have provided conflicting results. the effects likely depend on the probiotic strain and/or dosage. the aim of this study was to assess the effectiveness of lactobacillus reuteri dsm 17938 (l. reuteri; daily dose of 1 × 10 colony forming units) for preventing nosocomial diarrhea in children. | 2016 | 26551445 |
| immunomodulation of lactobacillus reuteri crl1324 on group b streptococcus vaginal colonization in a murine experimental model. | maternal group b streptococcus (gbs) colonization is a risk factor for infectious disease in newborns. one promising strategy is the modulation of vaginal defense to increase the host's ability to combat infection. | 2016 | 26547516 |
| characterization of the ecological role of genes mediating acid resistance in lactobacillus reuteri during colonization of the gastrointestinal tract. | rodent-derived strains of lactobacillus reuteri densely colonize the forestomach of mice and possess several genes whose predicted functions constitute adaptations towards an acidic environment. the objective of this study was to systematically determine which genes of l. reuteri 100-23 contribute to tolerance towards host gastric acid secretion. genes predicted to be involved in acid resistance were inactivated, and their contribution to survival under acidic conditions was confirmed in model g ... | 2016 | 26530032 |
| heat killed lactobacillus reuteri gmnl-263 reduces fibrosis effects on the liver and heart in high fat diet-hamsters via tgf-β suppression. | obesity is one of the major risk factors for nonalcoholic fatty liver disease (nafld), and nafld is highly associated with an increased risk of cardiovascular disease (cvd). scholars have suggested that certain probiotics may significantly impact cardiovascular health, particularly certain lactobacillus species, such as lactobacillus reuteri gmnl-263 (lr263) probiotics, which have been shown to reduce obesity and arteriosclerosis in vivo. in the present study, we examined the potential of heat-k ... | 2015 | 26516851 |
| the efficacy and safety of the probiotic bacterium lactobacillus reuteri dsm 17938 for infantile colic: a meta-analysis of randomized controlled trials. | to evaluate the efficacy and safety of lactobacillus reuteri dsm 17938 for treating infantile colic. | 2015 | 26509502 |
| characterization of the functional roles of amino acid residues in acceptor-binding subsite +1 in the active site of the glucansucrase gtf180 from lactobacillus reuteri 180. | α-glucans produced by glucansucrase enzymes hold strong potential for industrial applications. the exact determinants of the linkage specificity of glucansucrase enzymes have remained largely unknown, even with the recent elucidation of glucansucrase crystal structures. guided by the crystal structure of glucansucrase gtf180-δn from lactobacillus reuteri 180 in complex with the acceptor substrate maltose, we identified several residues (asp-1028 and asn-1029 from domain a, as well as leu-938, al ... | 2015 | 26507662 |
| infantile colic: recognition and treatment. | infantile colic is a benign process in which an infant has paroxysms of inconsolable crying for more than three hours per day, more than three days per week, for longer than three weeks. it affects approximately 10% to 40% of infants worldwide and peaks at around six weeks of age, with symptoms resolving by three to six months of age. the incidence is equal between sexes, and there is no correlation with type of feeding (breast vs. bottle), gestational age, or socioeconomic status. the cause of ... | 2015 | 26447441 |