| [study of megateriopeptidase, an exocellular peptidase of bacillus megaterium. 3. biosynthesis and physiological function]. | | 1969 | 4983765 |
| a novel protoplast-bursting factor (surfactin) obtained from bacillus subtilis iam 1213. i. the effects of surfactin on bacillus megaterium km. | | 1970 | 4984210 |
| structure of the peptidoglycan in escherichia coli b and bacillus megaterium km. stereospecific synthesis of two meso-diaminopimelic acid peptides with the tetrapeptide subunit of bacterial cell wall peptidoglycan. | | 1970 | 4984729 |
| effect of an azo dye on germination and outgrowth of bacillus megaterium spores. | | 1970 | 4984736 |
| thermal stimulation of bacillus megaterium glucose-6-phosphate dehydrogenase. | | 1970 | 4984936 |
| divalent cation activation of deoxycholate-solubilized and -inactivated membrane reduced nicotinamide adenine dinucleotide oxidase of bacillus megaterium km. | after treating bacillus megaterium km membranes with 0.2% sodium deoxycholate, most of the membrane reduced nicotinamide adenine dinucleotide (nadh) oxidase was inactivated, and all of the membrane nadh-2,6 dichlorophenol indophenol oxidoreductase was solubilized. dilution of the deoxycholate-treated membranes in the presence of divalent cations restored almost all of the original membrane nadh oxidase. the effectiveness of the divalent cation activation decreased in the order ba(2+) > ca(2+) > ... | 1970 | 4985543 |
| [storage of reserve substances in bacillus megaterium. ii. studies of poly-beta-hydroxybutyric acid-free mutants]. | | 1969 | 4985561 |
| [storage of reserve substances in bacillus megaterium. i. studies of wild strains]. | | 1969 | 4985562 |
| thiostrepton, an inhibitor of 5os ribosome subunit function. | thiostrepton inhibits (14)c-leucine incorporation by intact cells of bacillus megaterium as well as (14)c-phenylalanine incorporation by a poly u-directed extract of escherichia coli. extracts of e. coli which are pretreated by incubation with thiostrepton cannot be reactivated by dialysis to more than 5% of their former activity. the 50s ribosome subunit appears to be the site of thiostrepton action, since protein-synthesizing activity can be restored to dialyzed pretreated extracts by suppleme ... | 1970 | 4985587 |
| activation energy for glucose-induced germination of bacillus megaterium spores. | the maximum germination rate of bacillus megaterium qm b1551 spores in glucose increased, and the lag before its attainment decreased, with increasing germination temperature. the activation energy for germination (mu = approximately 20 kcal/mole), based on rate or on lag, was consistent with an enzymatic mechanism. | 1970 | 4987307 |
| factors affecting deoxycholate inactivation and mg++ reactivation of bacillus megaterium km membrane nicotinamide adenine dinucleotide (reduced form) oxidase. | decay of the reduced nicotinamide adenine dinucleotide oxidase of bacillus megaterium km membranes was prevented by storage in 10% (v/v) glycerol or 0.4% bovine serum albumin. differential rates of solubilization of components of the oxidase system by 0.4% deoxycholate was demonstrable at 4 c. the amount of mg(++) necessary for maximal oxidase reactivation increased with increasing amounts of deoxycholate-inactivated oxidase. mg(++) activation of deoxycholate-inactivated oxidase was partially te ... | 1970 | 4988244 |
| [bacterial spore coat. ii. chemical structure of fractions isolated from the spore coat of bacillus megaterium]. | | 1970 | 4988341 |
| studies on membrane synthesis in bacillus megaterium km. | | 1970 | 4988529 |
| [bacterial spore coat. 3. morphological changes of the spore coat during germination of bacillus megaterium]. | | 1970 | 4988792 |
| [bacterial spore coat. 4. chemical changes of the spore coat during germination of bacillus megaterium]. | | 1970 | 4988793 |
| kinetic studies of the in vivo exchange of ribosome subunits in bacillus megaterium. | | 1970 | 4989227 |
| [enrichment, purification and characterization of a bacteriocin from bacillus megaterium 337]. | | 1970 | 4989281 |
| assay and properties of diaminopimelate epimerase from bacillus megaterium. | 1. diaminopimelate epimerase from a soluble extract of bacillus megaterium n.c.i.b. 7581 was purified about 25-fold by fractionation with ammonium sulphate and chromatography on calcium phosphate gel-cellulose. the product was impure but was unstable on further purification. 2. quantitative assay methods for the enzyme were devised in which meso- or ll-diaminopimelic acid may be the substrate. 3. between 25 degrees and 45 degrees at ph7.0 enzyme action leads to an equilibrium mixture containing ... | 1969 | 4989322 |
| inhibition of bacillus megaterium by a trimethylamine oxide-associated browning reaction product. | heated combinations of trimethylamine oxide (tmao) and culture media (tryptone, glucose, yeast extract broth or a defined minimal medium), or heated tmao and glucose, contained substance(s) that inhibited growth of bacillus megaterium. inhibition was expressed primarily as an increase of the lag phase of growth; the logarithmic growth rate was comparable to control cultures. the addition of unheated tmao to the culture media had no effect on growth. results suggested that tmao was decomposed dur ... | 1970 | 4989667 |
| [investigations on some enzymes in sporulation of bacillus megaterium]. | | 1969 | 4990090 |
| a specific inhibitor of megacin a from bacillus megaterium producing megacin a. | | 1970 | 4990329 |
| properties of flagellin from bacillus megaterium km and its association with the cytoplasmic membrane. | | 1970 | 4990516 |
| regulation of formation of proteases in bacillus megaterium. iv. the formation of the enzyme in the sporogenous strain km. | | 1970 | 4990870 |
| mucopeptide metabolism during growth and sporulation in bacillus megaterium. | | 1970 | 4991643 |
| aflatoxin b1 induction of lysogenic bacteria. | a technique for biological verification of aflatoxin b(1) was developed based on toxin-mediated induction of lysis in a lysogenic strain of bacillus megaterium nnrl b-3695. reduction of culture turbidity was determined at various concentrations of toxin. incubation of 1.1 x 10(-4) g (dry weight) of cells/ml of growth medium containing 25 mug of b(1) per ml at 37 c reduced initial turbidity 0.20 absorbance units in 4 hr. if the bacterial lysate of the lysogenic strain, after a 2-hr incubation wit ... | 1970 | 4991922 |
| [mode of action of megacin cx on bacillus megaterium km]. | | 1970 | 4991989 |
| methylated bases of bacillus megaterium km nucleic acids: comparison with escherichia coli. | parallel studies were performed with methionineless derivatives of escherichia coli 15 t(-) and bacillus megaterium km: t(-). methylated bases are present in the total cell ribonucleic acid (rna) of b. megaterium. the level of rna methylation in e. coli is about 60% greater than that in b. megaterium. although e. coli deoxyribonucleic acid (dna) was found to contain 0.12% 5-methylcytosine (5-mc) and 0.24% 6-methylaminopurine (6-ma), methylated bases were not detected in the dna of b. megaterium. ... | 1970 | 4992370 |
| intracellular distribution of ribosomes and polyribosomes in bacillus megaterium. | | 1970 | 4992640 |
| neutron activation analysis of manganese and sodium in bacterial cells. | the application of neutron activation analysis for mineral determinations in bacteria was investigated. elements considered here were manganese and sodium. the sporeformer bacillus megaterium atcc 19213 was utilized. with this method, the manganese and sodium levels of whole and ashed vegetative cells, sporulating cells, and free spores were determined. the culture medium was also analyzed for these two elements. the results indicate that neutron activation analysis is readily applicable to the ... | 1970 | 4992658 |
| [genetic transformation of selenium resistance and selenium reductase formation in bacillus megaterium]. | | 1970 | 4992817 |
| biochemical alterations in bacillus megaterium as produced by aflatoxin b1. | bacillus megaterium nrrl b-1368 cells and spores were produced on trypticase soy broth (tsb) and agar (tsa) containing 3.8 mug of aflatoxin b(1) per ml, analyzed for selected chemical constituents, and compared to cells and spores of b. megaterium produced on nontoxic trypticase soy media. there was an initial 30% kill of cells after inoculation into toxic tsb and during the first 3.5 hr of incubation followed by a logarithmic growth phase in which the generation time was 75 min as compared to 2 ... | 1971 | 4993227 |
| morphological alterations in bacillus megaterium as produced by aflatoxin b1. | morphologically abnormal cells were produced by bacillus megaterium nrrl b-1368 in response to aflatoxin b(1). filamentous forms were characterized by early granulation and unusually large and numerous deposits of poly-beta-hydroxybutyric acid within the cells. pantoyl lactone was without effect as a reversing agent for the observed inhibition of cell septum formation. b. megaterium cells and spores produced on toxic (3.8 mug of aflatoxin b(1) per ml) and nontoxic trypticase soy broth and trypti ... | 1971 | 4993228 |
| gene dosage for 5s ribosomal ribonucleic acid in escherichia coli ad bacillus megaterium. | the number of gene copies for 5s ribosomal ribonucleic acid (rrna), relative to that for 16 and 23s rrna, has been determined by deoxyribonucleic acid (dna)-rna hybridization for escherichia coli and bacillus megaterium. in both cases, the number of 5s rrna genes equals the number of 16 or 23s rrna genes. rapid procedures for preparing extremely highly purified dna suitable for dna-rna hybridization experiments and chemically pure 5s rrna are described. | 1971 | 4993318 |
| development of bacteriophage alpha: requirement for the integrity of one strand of parental deoxyribonucleic acid. | bacteriophage alpha-bacillus megaterium complexes are as sensitive as free phage to the decay of (32)p incorporated in parental phage deoxyribonucleic acid during the eclipse period. | 1970 | 4993333 |
| iron requirements and aluminum sensitivity of an hydroxamic acid-requiring strain of bacillus megaterium. | bacillus megaterium strain atcc 19213 secretes a ferric-chelating secondary hydroxamic acid, whereas a mutant (strain sk11) derived from it cannot produce a hydroxamate. strain sk11 could be cultivated in a sucrose-mineral salts medium (treated with chelex 100 to reduce trace metals) in the absence of added hydroxamate, if the inoculum was high. the lowest iron supplements necessary for maximal growth of both strains were equivalent (0.01 to 0.04 mug of iron per ml). addition of either aluminum ... | 1971 | 4993339 |
| synthesis of glutamate by a glutamine: 2-oxo-glutarate amidotransferase (nadp oxidoreductase) in bacillus megaterium. | | 1971 | 4993401 |
| [characteristics of bacillus megaterium metabolism in a medium with citric acid as the sole carbon source]. | | 1969 | 4993449 |
| [the effect of bentonite on serratia marcescens and bacillus megaterium in a mineral medium with gelatin]. | | 1970 | 4993683 |
| [utilization of bacillus megaterium h for selection of antineoplastic substances of plant origin]. | | 1970 | 4993970 |
| selenomethionine, a methyl donor for bacterial nucleic acids. | dl-selenomethionine is as efficient a methyl donor for nucleic acid methylation in both bacillus megaterium km and escherichia coli wwu as dl-methionine. | 1971 | 4994033 |
| deoxynucleoside kinases of bacillus megaterium km. | dialyzed extracts of bacillus megaterium km contain thymidine, deoxyadenosine, and deoxyguanosine kinase activities. thymidine kinase activity is best with deoxyadenosine triphosphate or deoxyguanosine triphosphate (dgtp) as the phosphoryl donor, whereas the best deoxyadenosine kinase activity is obtained with dgtp or adenosine triphosphate. deoxyguanosine kinase activity functions optimally with deoxycytidine triphosphate as the donor. although the thymidine kinase activity of crude extracts do ... | 1971 | 4994037 |
| the production of protease by dense suspensions of bacillus megaterium km sp. | | 1971 | 4994996 |
| bacillus megaterium neutral protease, a zinc-containing metalloenzyme. | | 1971 | 4995315 |
| electron microscopic observations on growing and dividing protoplasts of bacillus megaterium. | | 1970 | 4995447 |
| comparison of galactokinase induction in protoplasts and intact bacteria of bacillus megaterium 216. | | 1970 | 4995448 |
| [characterization of a cytoplasmic endopeptidase in bacillus megaterium undergoing sporulation]. | | 1971 | 4995615 |
| the functioning of the membranes of the cells of mesophilic and thermophilic varieties of bacillus megaterium. | | 1971 | 4995843 |
| effect of glutaraldehyde on protoplasts of bacillus megaterium. | | 1970 | 4995902 |
| surface charges of two types of bacillus megaterium spores differing in their response to n-butane. | | 1970 | 4995903 |
| total counts of bacillus megaterium and clostridium butyricum spore suspensions using a microlitre syringe. | | 1970 | 4995937 |
| ultrastructure of spore coats produced by lethal germination of spores of bacillus megaterium 9885. | | 1970 | 4995939 |
| timing of mucopeptide and phospholipid synthesis in sporulating bacillus megaterium. | | 1971 | 4996163 |
| properties of the cytoplasmic membrane of bacillus megaterium km dissolved in various reagents. | | 1971 | 4998056 |
| [growth inhibition in bacillus megaterium by h+ and oh- ions]. | | 1970 | 4998104 |
| [cell division behavior of bacillus megaterium during transition to carbohydrate starvation]. | | 1971 | 4998213 |
| [sporulation behavior of bacillus megaterium during carbohydrate starvation]. | | 1971 | 4998214 |
| streptomycin resistance in bacillus megaterium. | | 1971 | 4998253 |
| [characteristics of the growth of a periodic culture of bacillus megaterium]. | | 1971 | 4998579 |
| utilization of glyoxylate by acetate-grown bacillus megaterium km. | | 1971 | 4999342 |
| molecular sieving by the bacillus megaterium cell wall and protoplast. | passive permeabilities of the cell wall and protoplast of bacillus megaterium strain km were characterized by use of 50 hydrophilic probing molecules (tritiated water, sugars, dextrans, glycols, and polyglycols) which varied widely in size. weight per cent uptake values (r(w)) were measured at diffusional equilibrium under conditions that negated the influences of adsorption or active transport. plots of r(w) for intact cells as a function of number-average molecular weight ( m(n)) or einstein-s ... | 1971 | 4999413 |
| role of carbon and nitrogen sources in bacterial growth and sporulation. | a simple growth medium is reported, in which bacillus megaterium forms heat-stable spores, heat-labile spores, or only vegetative cells by changing the carbon and nitrogen source. studies carried out in such media could be very useful in elucidating biochemical events which lead to bacterial sporulation. | 1971 | 4999972 |
| metabolic activity of acetate-grown bacillus megaterium km. | | 1970 | 5000287 |
| active transport of iron in bacillus megaterium: role of secondary hydroxamic acids. | kinetics of radioactive iron transport were examined in three strains of bacillus megaterium. in strain atcc 19213, which secretes the ferric-chelating secondary hydroxamic acid schizokinen, (59)fe(3+) uptake from (59)fecl(3) or the ferric hydroxamate desferal-(59)fe(3+) was rapid and reached saturation within 3 min. in strain sk11, which does not secrete schizokinen, transport from (59)fecl(3) was markedly reduced; the two ferric hydroxamates desferal-(59)fe(3+) or schizokinen-(59)fe(3+) increa ... | 1971 | 5000305 |
| bactericidal action of 2-hydroxy-3-(cyclohexylpropyl)-1,4-naphthoquinone on bacillus megaterium. | the antimalarial drug, 2-hydroxy-3-(cyclohexylpropyl)-1,4-naphthoquinone (nq), at concentrations of approximately 10(-5)m (3 mug/ml), was bactericidal for the gram-positive bacterium, bacillus megaterium. only a few other gram-positive bacteria were sensitive to this drug. all growth inhibitory concentrations of nq were also bactericidal for b. megaterium, and even resting suspensions of cells were killed. the incorporation of radioactive-labeled leucine, thymidine, uracil, and diaminopimelic ac ... | 1971 | 5000306 |
| ribonucleic acid synthesis in bacteria treated with toluene. | escherichia coli and bacillus megaterium rendered permeable to ribonucleoside triphosphates by toluene treatment retain the capacity to synthesize discrete ribonucleic acid species. | 1971 | 5000311 |
| some features of the bacterial membrane studied with the aid of a new fractionation technique. | membranes of bacillus megaterium and other bacteria were bound to crystals of cadmium lauroylsarcosinate and were resolved into nine lipoprotein fractions by elution with potassium chloride and sodium deoxycholate solutions. the fractions differed widely in protein/lipid ratio. some major protein species were probably common to all the fractions, but the phospholipid composition showed some variation. electron-microscopic examination after negative staining revealed that material in certain frac ... | 1971 | 5000708 |
| distribution and correlation of events during thermal inactivation of bacillus megaterium spores. | aqueous suspensions of bacillus megaterium qm b1551 spores were heated at temperatures from 75 to 85 c. the rapid initial viability loss, followed by a more gradual, almost exponential decline, was not due to mixed populations with discrete heat resistances. the slight "tailing" below 0.01% survival was not the result of heat adaptation. loss of viability was more rapid than loss of dipicolinic acid (dpa) and germinability and, although these events could not be correlated by use of simple kinet ... | 1971 | 5001193 |
| formation of -cyanoalanine by o-acetylserine sulfhydrylase. | cell-free extracts of bacillus megaterium form beta-cyanoalanine (beta-cna)-(14)c from na(14)cn and l-cysteine, o-acetyl-l-serine or, to a lesser extent, l-serine. however, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of beta-cna from cysteine and cyanide. the formation of beta-cna is readily detected in extracts of cells grown in synthetic media with sulfate or l-djenkolic acid as sulfur sources; such cells also exhibit a ... | 1971 | 5001194 |
| antimicrobial actions of hexachlorophene: lysis and fixation of bacterial protoplasts. | hexachlorophene was found to be both a lytic and a fixative agent for protoplasts isolated from bacillus megaterium. concentrations of 50 to 100 mug of drug per mg of original cell dry weight were required to lyse 4.4 x 10(9) protoplasts (2 mg of original cell dry weight). at higher drug concentrations, protoplasts became fixed against osmotic stress and reduced in sensitivity to disruption by n-butanol. lower drug concentrations caused proportionate lysis in the protoplast population. intact ce ... | 1971 | 5001202 |
| -aminobutyric acid as a required germinant for mutant spores of bacillus megaterium. | germinated spores of bacillus megaterium qm b1551 were irradiated with ultraviolet light, and spore-forming survivors were screened for germination requirements. spore strains which failed to germinate in a variety of defined solutions germinative for spores of the parent strain were obtained. mutant spores germinated readily in solutions containing yeast extract or one of numerous complex preparations. gamma-aminobutyric acid, obtained from yeast extract by column chromatography, was shown to b ... | 1971 | 5001872 |
| heat resistance of vegetative form and spore antigens of bacillus megaterium "in situ" and in "solution". | | 1970 | 5002842 |
| calcium transport in bacillus megaterium. | | 1971 | 5003592 |
| relationship of lysogeny and megacinogeny in bacillus megaterium. | | 1971 | 5004080 |
| effect of chlorpromazine on cell wall biosynthesis and incorporation of orotic acid into nucleic acids in bacillus megaterium. | | 1971 | 5004771 |
| [dna composition of bacillus megaterium variants obtained by the action of phages]. | | 1971 | 5005569 |
| aspects of the action of novobiocin on staphylococcus aureus and bacillus megaterium. | | 1971 | 5005820 |
| sporulation of bacillus megaterium: roles of metal ions. | | 1971 | 5005823 |
| influence of food microorganisms on staphylococcal growth and enterotoxin production in meat. | forty-four microorganisms were studied for their influence on staphylococcal growth and enterotoxin production. inhibition was found to be more common than stimulation. two types of inhibition were observed: inhibition of staphylococcal growth, and inhibition of enterotoxin formation with no apparent effect on growth. by use of a plate test, 12 of the 44 food microorganisms were found to inhibit staphylococcal growth at 35 c. of the 12, 3 also inhibited growth at 25 c. no significant differences ... | 1966 | 5970822 |
| resistivity of spores to ultraviolet and gamma radiation while exposed to ultrahigh vacuum or at atmospheric pressure. | viability studies were conducted on microbial spores subjected to ultrahigh vacuum (uhv) in the 10(-9) to 10(-10) torr range. after 5 to 7 days in vacuum, they were exposed to ultraviolet (uv) or to gamma radiation either while still under vacuum or in the presence of dried air. among the four test organisms subjected to uhv and ultraviolet radiation, aspergillus niger was the most resistant; bacillus megaterium, b. subtilis var. niger, and b. stearothermophilus were about equally less resistant ... | 1967 | 6035043 |
| the cell wall peptidoglycan of bacillus megaterium km. i. studies on the stereochemistry of alpha, alpha'-diaminopimelic acid. | | 1967 | 6058125 |
| [site-specific endonuclease bmei from bacillus megaterium 216]. | a site-specific endonuclease bmei has been isolated from bacillus megaterium 216 by gel filtration on ultragel aca-44 with a subsequent chromatography on heparin-sepharose 6b. on the double-stranded dna the endonuclease recognizes the pentanucleotide sequence (formula: see text); and hydrolyzes it in the points shown by arrows. at gel filtration the endonuclease is eluted in the volume corresponding to a molecular mass of 60 000. | 1983 | 6091684 |
| molecular cloning of structural and immunity genes for megacins a-216 and a-19213 in bacillus megaterium. | a host-vector system was developed for molecular cloning in bacillus megaterium and used to clone the structural and immunity genes for megacins a-216 and a-19213. recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. restriction mapping of native megacinogenic plasmids and recombinant clones was used to construct physical and genetic maps of megacinogenic plasmids pbm309 and pbm113. limited sequence homology between pbm309 and pbm113 w ... | 1984 | 6094510 |
| identification of the altered ribosomal component responsible for resistance to micrococcin in mutants of bacillus megaterium. | a mutant strain of bacillus megaterium, arising spontaneously and resistant to micrococcin , possesses ribosomes which contain an altered form of protein bm-l11 (the homologue of escherichia coli protein l11). reconstitution analysis has revealed that the alteration to protein bm-l11 is the sole cause of resistance in this strain. | 1984 | 6144543 |
| comparative macromolecular composition of filaments and rods of a bacillus megaterium thermoconditional morphological mutant. | filaments of a thermosensitive bacillus megaterium mutant showed an altered macromolecular composition compared with salt-cured mutant cells and parental cells. filaments contained more peptidoglycan, polyglucose, poly-beta-hydroxy-butyrate, and deoxyribonucleic acid per unit of protein. the ribonucleic acid-to-protein ratio of filaments was similar to that of rods or salt-cured cells. filament formation seemed to be due to defective protein or ribonucleic acid metabolism. | 1980 | 6158510 |
| improved purification and some properties of megacin cx, a bacteriocin produced by bacillus megaterium. | megacin cx is a bacteriocidal protein, previously described by durner and mach (durner, k. (1970) z. allg. mikrobiol. 10, 93-102; durner, k. (1970) z. allg. mikrobiol. 10, 373-382; durner, k., and mach, f. (1966) zentralbl. bakteriol. parasitenkd. infektionskr. hyg. abt. i orig. 2, 120, 565-575), which is released into the medium during growth by bacillus megaterium strain 337. we have optimized the culturing conditions to achieve reproducibly high yields of this protein; under these conditions, ... | 1981 | 6161120 |
| [delayed hypersensitivity reaction in mice immunized with bacillus megaterium h]. | | 1981 | 6164906 |
| electron microscopic cytochemical study of cell-wall polysaccharides in bacillus subtilis and two strains of bacillus megaterium. | | 1982 | 6183439 |
| [comparison of the lytic effect of gramicidin s and its derivatives on bacillus megaterium and micrococcus lysodeikticus protoplasts]. | essential differences were found between the lytic action of gramicidin s. on bacillus megaterium protoplasts and that on micrococcus lysodeikticus protoplasts. when protoplasts were suspended in a sucrose solution in phosphate buffer, the lytic activity of gramicidin s toward b. megaterium protoplasts increased with a rise in the antibiotic concentration; the lytic action of gramicidin s on m. lysodeikticus protoplasts was characterized by a complex concentration dependence. gramicidin s deriva ... | 1982 | 6184603 |
| [use of rifampicin for directed modification of the nucleotide composition of the cells of bacillus megaterium and candida utilis]. | the object of this work was to study the effect of rifampicin on the physiological state of bacillus megaterium and candida utilis in chemostat and batch cultures. when b. megaterium is cultivated in a medium containing 1 microgram of rifampicin per litre, its metabolism changes, the pool of free nucleotides rises, and the content of rna drops in response to the specific action of the inhibitor. the modified state is observed throughout many generations. the action of the inhibitor is also non-s ... | 1983 | 6188945 |
| cloning of a new low-molecular-weight spore-specific protein gene from bacillus megaterium. | three ecori fragments of bacillus megaterium dna hybridized only under nonrestrictive conditions on southern blots to a probe containing the previously cloned gene for protein c, a small, acid-soluble spore protein (sasp) from b. megaterium. all three fragments were cloned in escherichia coli cells in plasmid pbr325, and after being transferred to an e. coli expression vector, one of the fragments (c-3) directed the synthesis of a new small, acid-soluble spore protein (termed c-3) immunologicall ... | 1984 | 6199341 |
| extracellular siderophores from aspergillus ochraceous. | a large number of iron-chelating compounds (siderophores) were isolated from supernatants of iron-deficient cultures of a mold isolate, subsequently identified as aspergillus ochraceous . siderophores in their iron chelate form were purified to homogeneity by using bio-gel p2, silica gel, and c-18 bonded silica gel (reverse-phase) columns. most of these compounds, as identified by 1h and 13c nuclear magnetic resonance spectroscopy and x-ray crystallography, belong to the ferrichrome family. the ... | 1984 | 6233261 |
| an investigation of membrane fluidity changes during sporulation and germination of bacillus megaterium k.m. measured by electron spin and nuclear magnetic resonance spectroscopy. | changes in membrane and macromolecular fluidity which may accompany the differentiation processes of sporulation and germination in bacillus megaterium k.m. are examined by electron spin and nuclear magnetic resonance spectroscopy. no change in membrane lipid fluidity is observed in isolated forespores up to stage vi. between stage vi and release of mature spores, the esr spectrum of doxylstearic acid spin labels becomes polycrystalline. this change in spectral fluidity is completely reversed du ... | 1980 | 6250594 |
| megacinogenic plasmid from bacillus megaterium 216. | ability to produce megacin a, a bacteriocin of b. megaterium, was transferred from the strain b. megaterium 216 into auxotrophic derivatives of the strain b. megaterium km via protoplast fusion and polyethylene-glycol-induced protoplast transformation by plasmid dna, respectively. a 30.9 megadalton plasmid was detected in cells with mega phenotype, and the loss of this phenotype was accompanied in each case with the elimination of that plasmid. the megacinogenic plasmid pbm309 has a single site ... | 1980 | 6258024 |
| characterization of the plasmids of bacillus megaterium: restriction endonuclease digestions, southern blotting analysis, and partial denaturation mapping. | | 1980 | 6261279 |
| mp13, a generalized transducing bacteriophage for bacillus megaterium. | the first generalized transducing bacteriophage reported for bacillus megaterium has been characterized. optimum conditions for lysate production and transduction procedures were established so that transducing frequencies of 8 x 10(-6) and higher are now possible. the phage, mp13, has a head diameter of 97 nm and a contractile tail (202 by 17 nm) and adsorbs to the periphery of the cell. mp13 was inactivated rapidly at 60 degrees c, but not at 55 degrees c, and was sensitive to toluene, ether, ... | 1982 | 6277850 |
| studies on bacterial cell wall inhibitors. x. properties of phosph-n-acetylmuramoyl-pentapeptide-transferase in peptidoglycan synthesis of bacillus megaterium and its inhibition by amphomycin. | the phospho-n-acetylmuramoyl-pentapeptide-transferase from bacillus megaterium km was characterized by the transfer reaction. the particulate enzyme preparation had the activity to transfer phospho-n-acetylmuramoyl-pentapeptide from udp-n-acetylmuramoyl-pentapeptide to undecaprenoid-1-ol-phosphate. the optimum ph for activity was about 8.5. the reaction required the presence of mg2+ and an sh-protector. with 25 mm mg2+ the maximum activity was observed. the reaction was reversible and so the add ... | 1982 | 6292151 |
| cloning of the gene for c protein, a low molecular weight spore-specific protein from bacillus megaterium. | the structural gene for c protein, a low molecular weight spore-specific protein from bacillus megaterium, has been cloned in escherichia coli. expression of the c-protein gene in e. coli requires an external transcription promoter and prevention of termination of transcription prior to transcription of all or part of the sequence coding for the c protein. the gene for the c protein is within a 5-kilobase dna fragment, but this fragment does not code for either of the other two major low molecul ... | 1983 | 6304701 |
| deletion mutants of megacinogenic plasmid pbm309 from bacillus megaterium. | a 46.8-kb plasmid, pbm309, of bacillus megaterium determines the production of a bacteriocin, megacin a, and confers immunity against this antibiotic on the host cells. the megacin a (mega) and megacin a-immunity (megaim) genes were mapped on the physical map of pbm309 by using its deletion derivatives. both genes were isolated as a 10.6-kb psti fragment and cloned in bacillus subtilis vector plasmid pbd9 for expression in b. megaterium. | 1983 | 6319232 |
| complete nucleotide sequence and start sites for transcription and translation of the bacillus megaterium protein c gene. | the nucleotide sequence of the bacillus megaterium protein c gene, encompassing the coding region and 341 base pairs of flanking regions, has been determined. the gene codes for a 72-residue protein whose predicted amino acid sequence is identical to that previously determined for protein c with the exception of an amino-terminal methionine predicted from the gene sequence, but not found in the mature protein. the translational initiation codon is preceded by an 11-base pair sequence highly comp ... | 1984 | 6327639 |
| combination of misonidazole and lipoic acid on the radiation sensitivity of bacillus megaterium spores. | the radiation sensitizing action of a combination of misonidazole and lipoic acid was investigated in bacillus megaterium spores at various oxygen concentrations. lipoic acid and misonidazole were applied in two concentrations (0.1 and 1.0 mmol/l) and four combinations were prepared from them. no uniform correlation was found, neither to the combination of the compounds nor to the gas conditions. the combination of two radiation sensitizing compounds with in all probability different action mech ... | 1984 | 6328893 |