| beneficial rhizobacterium bacillus amyloliquefaciens sqr9 induces plant salt tolerance through spermidine production. | the inoculation of plants with plant growth-promoting rhizobacterium (pgpr) has been an effective strategy for enhancing plant salt tolerance to diminish the loss of agricultural productivity caused by salt stress, but the signal transmitted from bacteria to the plant under salt stress is poorly understood. in this study, the salt tolerance of arabidopsis thaliana and zea mays was enhanced by inoculation with bacillus amyloliquefaciens sqr9. using dialysis bags with different molecular weight cu ... | 2017 | 28291380 |
| effects of probiotic bacillus as a substitute for antibiotics on antioxidant capacity and intestinal autophagy of piglets. | the objective of this study was to evaluate effects of probiotic bacillus amyloliquefaciens (ba) as a substitute for antibiotics on growth performance, antioxidant ability and intestinal autophagy of piglets. ninety piglets were divided into three groups: g1 (containing 150 mg/kg aureomycin in the diet); g2 (containing 75 mg/kg aureomycin and 1 × 10(8) cfu/kg ba in the diet); g3 (containing 2 × 10(8) cfu/kg ba in the diet without any antibiotics). each treatment had three replications of ten pig ... | 2017 | 28244029 |
| beneficial effects of fermented sardinelle protein hydrolysates on hypercaloric diet induced hyperglycemia, oxidative stress and deterioration of kidney function in wistar rats. | this study investigated the potential effects of fermented sardinelle protein hydrolysates (fsphs) obtained by two proteolytic bacteria, bacillus subtilis a26 (fsph-a26) and bacillus amyloliquefaciens an6 (fsph-an6), on hypercaloric diet (hcd) induced hyperglycemia and oxidative stress in rats. effects of fsphs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. biochemical findings r ... | 2017 | 28242930 |
| combinatorial effect of mutagenesis and medium component optimization on bacillus amyloliquefaciens antifungal activity and efficacy in eradicating botrytis cinerea. | this work is directed towards bacillus amyloliquefaciens strain blb371 metabolite production for biocontrol of fungal phytopathogens. in order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. after three rounds of mutagenesis, a hyper active mutant, named m3-7, was obtained. it produces 7 fold more antifungal metabolites (1800au/ml) than the wild strain in mc medium. a hybrid design was applied to opt ... | 2017 | 28219523 |
| high-level extracellular protein production in bacillus subtilis using an optimized dual-promoter expression system. | we recently constructed a bacillus subtilis strain (cctcc m 2016536) from which we had deleted the srfc, spoiiac, npre, apre and amye genes. this strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, pamyq from bacillus amyloliquefaciens, should improve protein expression using this strain. this study was undertaken ... | 2017 | 28219382 |
| antifungal effect of volatile organic compounds produced by bacillus amyloliquefaciens cpa-8 against fruit pathogen decays of cherry. | the present work focuses on the antifungal effect of volatile organic compounds (vocs) produced by bacillus amyloliquefaciens cpa-8 against monilinia laxa, m. fructicola and botrytis cinera, three postharvest fruit pathogens of sweet cherry fruit. vocs were evaluated with a double petri dish assay against mycelial and colony growth of target pathogens. for this purpose, cpa-8 was grown on different media and cultured for 24 and 48 h at 30 °c before assays. data showed that mycelial growth inhibi ... | 2017 | 28213029 |
| selection of bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets. | we have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. the spore-forming bacillus species are widely used as probiotics in feed supplements for pigs. in this study, we evaluated the option for further functionalizing bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. we established ... | 2017 | 28204884 |
| improvement of levan production in bacillus amyloliquefaciens through metabolic optimization of regulatory elements. | levan is a functional homopolymer of fructose with considerable applications in food, pharmaceutical and cosmetic industries. to improve the levan production in bacillus amyloliquefaciens, the regulatory elements of sacb (encoding levansucrase) expression and levansucrase secretion were optimized. four heterologous promoters were evaluated for sacb expression, and the pgrac promoter led to the highest level for both sacb transcription and levansucrase enzyme activity. the levan production in the ... | 2017 | 28197690 |
| bacillus spp. isolated from puba as a source of biosurfactants and antimicrobial lipopeptides. | several products of industrial interest are produced by bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. this study aimed to investigate the potential of two bacterial isolates (p5 and c3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. phylogenetic analyses showed close relation of isolates p5 and c3 with bacillus amyloliquefaciens and bacillus thuringie ... | 2017 | 28197131 |
| loap is a broadly conserved antiterminator protein that regulates antibiotic gene clusters in bacillus amyloliquefaciens. | a valuable resource available in the search for new natural products is the diverse microbial life that spans the planet. a large subset of these microorganisms synthesize complex specialized metabolites exhibiting biomedically important activities. a limiting step to the characterization of these compounds is an elucidation of the genetic regulatory mechanisms that oversee their production. although proteins that control transcription initiation of specialized metabolite gene clusters have been ... | 2017 | 28191883 |
| application of bacteria from non-cultivated plants to promote growth, alter root architecture and alleviate salt stress of cotton. | cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. this process also eliminates beneficial bacteria on the seed surface. the goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt stress alleviating effects in seedlings. | 2017 | 28176460 |
| genomic and metabolic traits endow bacillus velezensis cc09 with a potential biocontrol agent in control of wheat powdery mildew disease. | bacillus velezensis cc09, which was isolated from healthy leaves of cinnamomum camphora and previously identified as bacillus amyloliquefaciens cc09, shows great potential as a new biocontrol agent, in control of many phytopathogenic diseases. to extend our understanding of the potential antifungal capacities, we did a whole genome analysis of strain cc09. result shows that strain cc09 has a relatively large genome size (4.17mb) with an average gc content of 46.1%, and 4021 predicted genes. thir ... | 2017 | 28164794 |
| acetylation and deacetylation for sucralose preparation by a newly isolated bacillus amyloliquefaciens wzs01. | sucralose is a non-nutritive artificial sweetener used in a broad range of foods and beverages. in the present study, bacillus amyloliquefaciens wzs01 was isolated, identified, and used as a catalyst both in regioselective acylation and deacetylation for sucralose preparation. bacterial cells were immobilized on polyurethane foam and utilized to synthesize sucrose-6-acetate regioselectively. the yield of sucrose-6-acetate was >95% with 60 mm sucrose after 22 h of reaction. free cells could hydro ... | 2017 | 28131539 |
| production and characterization of organic solvent-tolerant cellulase from bacillus amyloliquefaciens ak9 isolated from hot spring. | a cellulase-producing bacterium, designated as strain ak9, was isolated from a hot spring of tatta pani, azad kashmir, pakistan. the bacterium was identified as bacillus amyloliquefaciens through 16s rrna sequencing. cellulase from strain ak9 was able to liberate glucose from soluble cellulose and carboxymethyl cellulose (cmc). enzyme was purified through size exclusion chromatography and a single band of ∼47 kda was observed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page ... | 2017 | 28130767 |
| heterologous expression and enzymatic characterization of γ-glutamyltranspeptidase from bacillus amyloliquefaciens. | γ-glutamyltranspeptidase (ggt) catalyzes the cleavage of γ-glutamyl compounds and the transfer of γ-glutamyl moiety to water or to amino acid/peptide acceptors. ggt can be utilized for the generation of γ-glutamyl peptides or glutamic acid, which are used as food taste enhancers. in the present study, bacillus amyloliquefaciens smb469 with high ggt activity was isolated from doenjang, a traditional fermented soy food of korea. the gene encoding ggt from b. amyloliquefaciens smb469 (baggt469) was ... | 2017 | 28120195 |
| genome and transcriptome analysis of surfactin biosynthesis in bacillus amyloliquefaciens mt45. | natural bacillus isolates generate limited amounts of surfactin (<10% of their biomass), which functions as an antibiotic or signalling molecule in inter-/intra-specific interactions. however, overproduction of surfactin in bacillus amyloliquefaciens mt45 was observed at a titre of 2.93 g/l, which is equivalent to half of the maximum biomass. to systemically unravel this efficient biosynthetic process, the genome and transcriptome of this bacterium were compared with those of b. amyloliquefacien ... | 2017 | 28112210 |
| combinatorial effect of bacillus amyloliquefaciens ag1 biosurfactant and bacillus thuringiensis vip3aa16 toxin on spodoptera littoralis larvae. | spodoptera littoralis, one of the most serious and destructive agricultural pests in the world, is very susceptible to vip3 toxin. in order to develop a new efficient bioinsecticide and to prevent the development of resistance by the target pest, insecticidal activity of biosurfactant produced by bacillus amyloliquefaciens ag1 was evaluated against s. littoralis. bioassays revealed the susceptibility of the first instar larvae of this pest to ag1 biosurfactant with an lc50 of 245ng/cm(2). moreov ... | 2017 | 28093209 |
| adaptation of primocane fruiting raspberry plants to environmental factors under the influence of bacillus strains in western siberia. | in geographical locations with a short vegetative season and continental climate that include western siberia, growing primocane fruiting raspberry varieties becomes very important. however, it is necessary to help the plants to overcome the environmental stress factors. this study aimed to evaluate the impact of the pre-planting treatment of primocane fruiting raspberry root system with bacillus strains on the following plant development under variable environmental conditions. in 2012, bacillu ... | 2017 | 28092002 |
| isotryptophan from antarctic bacillus amyloliquefaciens pc3: purification, identification, characterization, and antifungal activity. | strain pc3 was isolated from antarctic seawater and identified as bacillus amyloliquefaciens. a compound with antifungal activity was purified from the fermentation supernatant of b. amyloliquefaciens pc3. its structure was determined to be (s)-2-amino-3-(1h-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (nmr) and high-resolution electrospray ionisation mass spectrometry (hr-esi-ms). isotryptophan exhibited good thermal stability ... | 2017 | 28044456 |
| design, synthesis and antibacterial activities of thiouracil derivatives containing acyl thiourea as seca inhibitors. | a series of novel thiouracil derivatives containing an acyl thiourea moiety (7a-7x) have been synthesized by structural modification of a lead seca inhibitor, 2. all the compounds have been evaluated for their antibacterial activities against bacillus amyloliquefaciens, staphylococcus aureus, and bacillus subtilis. compounds 7c, 7m, 7u, 7v exhibited promising activities against above bacteria. such four compounds were further tested for their inhibitory activity against seca atpase, and the resu ... | 2016 | 28041832 |
| potential of a new strain of bacillus amyloliquefaciens buz-14 as a biocontrol agent of postharvest fruit diseases. | the biocontrol potential of the bacillus amyloliquefaciens strain buz-14 was tested against the main postharvest diseases of orange, apple, grape and stone fruit. after characterizing the temperature and ph growth curves of strain buz-14, its in vitro antifungal activity was determined against botrytis cinerea, monilinia fructicola, m. laxa, penicillium digitatum, p. expansum and p. italicum. subsequently, in vivo activity was tested against these pathogens by treating fruit with cells, endospor ... | 2017 | 28040156 |
| design, synthesis and antimicrobial activities of thiouracil derivatives containing triazolo-thiadiazole as seca inhibitors. | a series of novel thiouracil derivatives containing a triazolo-thiadiazole moiety (7a-7l) have been synthesized by structural modifications on a lead seca inhibitor, 2. all the compounds have been evaluated for their antibacterial activities against bacillus amyloliquefaciens, staphylococcus aureus, and bacillus subtilis. compounds 7d and 7g were also tested for their inhibitory activities against seca atpase due to their promising antimicrobial activities. the inhibitory activity of compound 7d ... | 2017 | 28039774 |
| decreased formation of branched-chain short fatty acids in bacillus amyloliquefaciens by metabolic engineering. | to reduce the unpleasant odor during 1-deoxynojirimycin (dnj) production, the genes of leucine dehydrogenase (bcd) and phosphate butryltransferase (ptb) were deleted from bacillus amyloliquefaciens hz-12, and the concentrations of branched-chain short fatty acids (bcfas) and dnj were compared. | 2017 | 27999972 |
| high-level heat resistance of spores of bacillus amyloliquefaciens and bacillus licheniformis results from the presence of a spova operon in a tn1546 transposon. | bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. some spores may survive heating processes aimed at production of commercially sterile foods. recently, it was shown that a spova operon, designated spova(2mob), present on a tn1546 transposon in bacillus subtilis, leads to profoundly increased wet heat resistance of b. subtilis spores. such tn1546 transposon elements including the spova(2mob) operon were also found in several str ... | 2016 | 27994575 |
| spore-forming bacteria responsible for food spoilage. | this review explores the main spore-forming bacteria involved in the spoilage of various processed foods. bakery products are specifically spoiled by bacillus species, the dominant one being bacillus amyloliquefaciens, while different clostridium species classically contaminate refrigerated vacuum-packed meats. these two genera have also been isolated from milk products, even when milk is pasteurized, sterilized, dehydrated or fermented, according to heat treatment and storage temperature. final ... | 2016 | 27989764 |
| bacillus amyloliquefaciens sc06 alleviates the oxidative stress of ipec-1 via modulating nrf2/keap1 signaling pathway and decreasing ros production. | oxidative stress (os) plays a major role in the gastrointestinal disorders. although probiotics were reported to repress os, few researches compared the antioxidant ability of different bacillus strains and deciphered the mechanisms. to select a bacillus strain with higher antioxidant capacity, we used h2o2 to induce intestinal porcine epithelial cell 1 (ipec-1) os model. the most suitable h2o2 concentration and incubation time were determined by the half lethal dose and methyl thiazolyl tetrazo ... | 2017 | 27957629 |
| molecular dynamics and protein interaction studies of lipopeptide (iturin a) on α- amylase of spodoptera litura. | the small mottled willow moth (spodoptera litura) is one of the best-known agricultural pest insects. to understand the insecticidal activity, we have selected iturin a compound produced by bacillus amyloliquefaciens rhnk22 which showed the strongest and most common inhibitory effect on the spodoptera litura protein. in this work we have identified the action of iturin a on α- amylase is a major digestive enzyme of spodoptera litura using docking studies. a 3d model of α- amylase from spodoptera ... | 2017 | 27940096 |
| identification of root-secreted compounds involved in the communication between cucumber, the beneficial bacillus amyloliquefaciens, and the soil-borne pathogen fusarium oxysporum. | colonization of plant growth-promoting rhizobacteria (pgpr) is critical for exerting their beneficial effects on the plant. root exudation is a major factor influencing the colonization of both pgpr and soil-borne pathogens within the root system. however, the tripartite interaction of pgpr, plant roots, and soil-borne pathogens is poorly understood. we screened root exudates for signals that mediate tripartite interactions in the rhizosphere. in a split-root system, we found that root colonizat ... | 2017 | 27937752 |
| a trojan-horse peptide-carboxymethyl-cytidine antibiotic from bacillus amyloliquefaciens. | microcin c and related antibiotics are trojan-horse peptide-adenylates. the peptide part is responsible for facilitated transport inside the sensitive cell, where it gets processed to release a toxic warhead-a nonhydrolyzable aspartyl-adenylate, which inhibits aspartyl-trna synthetase. adenylation of peptide precursors is carried out by mccb thif-type nad/fad adenylyltransferases. here, we describe a novel microcin c-like compound from bacillus amyloliquefaciens. the b. amyloliquefaciens mccb de ... | 2016 | 27934031 |
| parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade-offs. | parasites and competitors are important for regulating pathogen densities and subsequent disease dynamics. it is, however, unclear to what extent this is driven by ecological and evolutionary processes. here, we used experimental evolution to study the eco-evolutionary feedbacks among ralstonia solanacearum bacterial pathogen, ralstonia-specific phage parasite, and bacillus amyloliquefaciens competitor bacterium in the laboratory and plant rhizosphere. we found that while the phage had a small e ... | 2017 | 27925169 |
| clarification of the antagonistic effect of the lipopeptides produced by bacillus amyloliquefaciens bpd1 against pyricularia oryzae via in situ maldi-tof ims analysis. | this study tried to clarify the antagonistic effect of the lipopeptides secreted by bacillus amyloliquefaciens strain bpd1 (ba-bpd1) against pyricularia oryzae cavara (po). to determine the major antifungal lipopeptides effective against po, single and dual cultures were carried out in solid-state media. the matrix-assisted laser desorption/ionization-time of flight imaging mass spectrometry (maldi-tof ims) was used to identify the most effective lipopeptide in situ. meanwhile, the morphology of ... | 2016 | 27918491 |
| production of bacillus amyloliquefaciens og and its metabolites in renewable media: valorisation for biodiesel production and p-xylene decontamination. | biosurfactants are important in many areas; however, costs impede large-scale production. this work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of bacillus amyloliquefaciens. initially, bacillus sp. strain 0g was renamed b. amyloliquefaciens subsp. plantarum (syn. bacillus velezensis) after analysis of the gyra and gyrb dna sequences. growth in modified landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and aceto ... | 2017 | 27912317 |
| bacillus species (bt42) isolated from coffea arabica l. rhizosphere antagonizes colletotrichum gloeosporioides and fusarium oxysporum and also exhibits multiple plant growth promoting activity. | colletotrichum and fusarium species are among pathogenic fungi widely affecting coffea arabica l., resulting in major yield loss. in the present study, we aimed to isolate bacteria from root rhizosphere of the same plant that is capable of antagonizing colletotrichum gloeosporioides and fusarium oxysporum as well as promotes plant growth. | 2016 | 27863465 |
| synthesis and characterization of silver nanoparticles using bacillus amyloliquefaciens and bacillus subtilis to control filarial vector culex pipiens pallens and its antimicrobial activity. | culex pipiens pallens are the most common mosquito's vector in asia. in order to protect the people from diseases, the anti-mosquito population is necessary that uses safe and new bio-pesticides such as bacteria-agnps. in our report, we used two kinds of bacteria to synthesize silver nanoparticles to examine the toxic effect on the larvae and pupae of cx. pipiens pallens and also used as antimicrobial activity. the biosynthesis of agnps and its characterization was carried out by uv-vis spectrop ... | 2016 | 27855517 |
| isolation and biochemical characterisation of a bacteriocin-like substance produced by bacillus amyloliquefaciens an6. | this study focuses on the isolation and characterisation of a peptide with bacteriocin-like properties from bacillus amyloliquefaciens an6. incubation conditions were optimised, and the effects of the incubation period and of carbon and nitrogen sources were investigated. the produced bacteriocin was partially purified with ammonium sulphate precipitation, dialysis and ultrafiltration and was then biochemically characterised. maximum bacteriocin production was achieved after 48h of incubation in ... | 2015 | 27842869 |
| identification of strain isolated from dates (phœnix dactylifera l.) for enhancing very high gravity ethanol production. | ethanol production from by-products of dates in very high gravity was conducted in batch fermentation using two yeasts, saccharomyces cerevisiae and zygosaccharomyces rouxii, as well as a native strain: an osmophilic strain of bacteria which was isolated for the first time from the juice of dates (phoenix dactylifera l.). the phylogenetic analysis based on the 16s ribosomal rna and gyrb sequence and physiological analysis indicated that the strain identified belongs to the genus of bacillus, b. ... | 2016 | 27838909 |
| effects of the dietary supplementation of mixed probiotic spores of bacillus amyloliquefaciens 54a, and bacillus pumilus 47b on growth, innate immunity and stress responses of striped catfish (pangasianodon hypophthalmus). | the study used the mixed probiotics of bacillus amyloliquefaciens 54a and b. pumilus 47b isolated from striped catfish (pangasianodon hypophthalmus) intestine aiming to stimulate growth performance, innate immunity, stress tolerance of striped catfish. the average weight gain (awg), specific growth rate (sgr), and feed conversion ratio (fcr) were analyzed after fish were fed the mixture of probiotics (b. amyloliquefaciens 54a and b. pumilus 47b) at concentrations of 1 × 10(8), 3 × 10(8), and 5 × ... | 2017 | 27836719 |
| use of bacillus amyloliquefaciens hz-12 for high-level production of the blood glucose lowering compound, 1-deoxynojirimycin (dnj), and nutraceutical enriched soybeans via fermentation. | 1-deoxynojirimycin (dnj) is an efficient α-glucosidase inhibitor (α-gi) with potential applications in the prevention and treatment of diabetes. in this study, 16 bacillus strains were screened for α-gi rate, and the strain hz-12 with the highest α-gi rate was identified as bacillus amyloliquefaciens through the analysis of physiological biochemical characteristics and 16s rdna sequence. by lc-ms/q-tof analysis, the α-gi component produced by b. amyloliquefaciens hz-12 was identified as dnj. soy ... | 2017 | 27826807 |
| improved growth and metabolite accumulation in codonopsis pilosula (franch.) nannf. by inoculation of bacillus amyloliquefaciens gb03. | codonopsis pilosula (franch.) nannf. is a traditional chinese herbal medicinal plant and a low-cost succedaneum for panax ginseng and contains various bioactivity components. in this work, we first evaluated the effects of the inoculation of the plant growth-promoting rhizobacteria bacillus amyloliquefaciens strain gb03 on growth and metabolite accumulation of c. pilosula. the results demonstrated that application of b. amyloliquefaciens gb03 significantly improved the growth of c. pilosula comp ... | 2016 | 27723315 |
| establishment and application of an analytical in-house database (ihdb) for rapid discrimination of bacillus subtilis group (bsg) using whole-cell maldi-tof ms technology. | members of the bacillus subtilis group (bsg) possess industrial applicability; unfortunately, b. subtilis and its phylogenetically closest species are indistinguishable from one another using 16s rdna sequencing, physiological and biochemical tests. matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is a relatively novel technique for the fast and reliable identification of microorganisms. the aim of this study was to construct a unique analytical in-hous ... | 2016 | 27507023 |
| properties of a fibrinolytic enzyme secreted by bacillus amyloliquefaciens rsb34, isolated from doenjang. | nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional korean fermented soy food. among them, rsb 34 showed the strongest activity and identified as bacillus amyloliquefaciens by 16s rrna and reca genes sequencing. during growth on lb up to 96 h, rsb34 showed the highest fibrinolytic activity (83.23 mu/μl) at 48 h. three bands of 23, 27, and 42 kda in size were observed when culture supernatant was analyzed by sds-page and 27 and 42 kda bands by fibrin zymography. a ... | 2016 | 27780963 |
| biological potential of bioorganic fertilizer fortified with bacterial antagonist for the control of tomato bacterial wilt and the promotion of crop yields. | the application of bacillus sp. in the biological control of plant soilborne diseases has been shown to be an environmentally friendly alternative to the use of chemical fungicides. in this study, the effects of bioorganic fertilizer (bof) fortified with bacillus amyloliquefaciens sqy 162 on the suppression of tomato bacterial wilt were investigated in pot experiments. the disease incidence of tomato wilt after the application of bof was 65.18% and 41.62% lower at 10 and 20 days after transplant ... | 2016 | 27381335 |
| activity, stability and 3-d structure of the cu(ii) form of a chitin-active lytic polysaccharide monooxygenase from bacillus amyloliquefaciens. | the enzymatic deconstruction of recalcitrant polysaccharide biomass is central to the conversion of these substrates for societal benefit, such as in biofuels. traditional models for enzyme-catalysed polysaccharide degradation involved the synergistic action of endo-, exo- and processive glycoside hydrolases working in concert to hydrolyse the substrate. more recently this model has been succeeded by one featuring a newly discovered class of mononuclear copper enzymes: lytic polysaccharide monoo ... | 2016 | 27722375 |
| effect of fructose on promoting fengycin biosynthesis in bacillus amyloliquefaciens fmb-60. | to explore the influence of carbohydrates on the production of fengycin by bacillus amyloliquefaciens fmb-60. | 2016 | 27611603 |
| artificial induction of genetic competence in bacillus amyloliquefaciens isolates. | to induce natural genetic competence in bacillus amyloliquefaciens isolates through overexpression of the master regulator, comk, from b. subtilis (comk bsu ). | 2016 | 27578391 |
| antioxidant and neuroprotective effects of doenjang prepared with rhizopus, pichia, and bacillus. | a new type of doenjang was manufactured by mixing soaked soybean, koji (rhizopus oryzae), cheonggukjang (bacillus amyloliquefaciens mj1-4 and b. amyloliquefaciens emd17), and pichia farinosa sy80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with r. oryzae. the mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. the extract of mixed culture doenjang showed h ... | 2016 | 27752498 |
| biological activities of peptide concentrates obtained from hydrolysed eggshell membrane byproduct by optimisation with response surface methodology. | the increase of hen egg consumption demands profitable applications for eggshells, including their membranes, in order to minimize environmental and public health problems that could result from their accumulation. this work presents an innovative application for eggshell membranes to obtain an added-value food ingredient that combines maximized ace-inhibitory and antioxidant activities. firstly, the use of acetic acid 5% (v/v); and 3-mercaptopropionic acid 1.25 m enabled 63% recovery of eggshel ... | 2016 | 27747356 |
| phes (*) , an effective host-genotype-independent counter-selectable marker for marker-free chromosome deletion in bacillus amyloliquefaciens. | aside from applications in the production of commercial enzymes and metabolites, bacillus amyloliquefaciens is also an important group of plant growth-promoting rhizobacteria that supports plant growth and suppresses phytopathogens. a host-genotype-independent counter-selectable marker would enable rapid genetic manipulation and metabolic engineering, accelerating the study of b. amyloliquefaciens and its development as both a microbial cell factory and plant growth-promoting rhizobacteria. here ... | 2017 | 27730334 |
| biological characteristics of bacillus amyloliquefaciens ak-0 and suppression of ginseng root rot caused by cylindrocarpon destructans. | the effect of bacillus amyloliquefaciens ak-0 (ak-0) on ginseng root rot disease caused by cylindrocarpon destructans was investigated. | 2017 | 27727491 |
| antibacterial polyketides from bacillus amyloliquefaciens associated with edible red seaweed laurenciae papillosa. | heterotrophic bacillus amyloliquefaciens associated with edible red seaweed, laurenciae papillosa was used to isolate antibacterial polyketide compounds. antibacterial activity studies integrated with the outcome obtained by polyketide synthetase (pks) coding genes established that seaweed-affiliated bacterial flora had a wide-ranging antibacterial activities and potential natural product diversity, which proved that the bacterium is valuable reservoir of novel bioactive metabolites. bioactivity ... | 2017 | 27719931 |
| a recessive pollination control system for wheat based on intein-mediated protein splicing. | a transgene-expression system for wheat that relies on the complementation of inactive precursor protein fragments through a split-intein system is described. the n- and c-terminal fragments of a barnase gene from bacillus amyloliquifaciens were fused to intein sequences from synechocystis sp. and transformed into wheat plants. upon translation, both barnase fragments are assembled by an autocatalytic intein-mediated trans-splicing reaction, thus forming a cytotoxic enzyme. this chapter focuses ... | 2017 | 27714617 |
| melatonin-producing endophytic bacteria from grapevine roots promote the abiotic stress-induced production of endogenous melatonin in their hosts. | endophytes form symbiotic relationships with plants and constitute an important source of phytohormones and bioactive secondary metabolites for their hosts. to date, most studies of endophytes have focused on the influence of these microorganisms on plant growth and physiology and their role in plant defenses against biotic and abiotic stressors; however, to the best of our knowledge, the ability of endophytes to produce melatonin has not been reported. in the present study, we isolated and iden ... | 2016 | 27708652 |
| whole genome shotgun sequence of bacillus amyloliquefaciens tf28, a biocontrol entophytic bacterium. | bacillus amyloliquefaciens tf28 is a biocontrol endophytic bacterium that is capable of inhibition of a broad range of plant pathogenic fungi. the strain has the potential to be developed into a biocontrol agent for use in agriculture. here we report the whole-genome shotgun sequence of the strain. the genome size of b. amyloliquefaciens tf28 is 3,987,635 bp which consists of 3754 protein-coding genes, 65 tandem repeat sequences, 47 minisatellite dna, 2 microsatellite dna, 63 trna, 7rrna, 6 srna ... | 2016 | 27688836 |
| bacillus amyloliquefaciens sb14 from rhizosphere alleviates rhizoctonia damping-off disease on sugar beet. | the use of biocontrol strains recently has become a popular alternative to conventional chemical treatments. a set of bacteria isolated from sugar beet rhizosphere and from roots and shoots of apple and walnut were evaluated for their potential to control sugar beet seedling damping-off caused by r. solani ag-4 and ag2-2.the results of in vitro assays concluded that three isolates, sb6, sb14, sb15, obtained from rhizosphere of sugar beet and five isolates, ap2, ap4, ap6, ap7, ap8, obtained from ... | 2016 | 27664740 |
| antagonist effects of bacillus spp. strains against fusarium graminearum for protection of durum wheat (triticum turgidum l. subsp. durum). | bacillus species are attractive due to their potential use in the biological control of fungal diseases. bacillus amyloliquefaciens strain blb369, bacillus subtilis strain blb277, and paenibacillus polymyxa strain blb267 were isolated and identified using biochemical and molecular (16s rdna, gyra, and rpob) approaches. they could produce, respectively, (iturin and surfactin), (surfactin and fengycin), and (fusaricidin and polymyxin) exhibiting broad spectrum against several phytopathogenic fungi ... | 2016 | 27664733 |
| characterization and evaluation of bacillus amyloliquefaciens strain wf02 regarding its biocontrol activities and genetic responses against bacterial wilt in two different resistant tomato cultivars. | bacillus amyloliquefaciens strain wf02, isolated from soil collected at wufeng mountain, taiwan, has siderophore-producing ability and in vitro antagonistic activity against bacterial wilt pathogen. to determine the impact of plant genotype on biocontrol effectiveness, we treated soil with this strain before infecting susceptible (l390) and moderately resistant (micro-tom) tomato cultivars with ralstonia solanacearum strain pss4. we also compared the efficacy of this strain with that of commerci ... | 2016 | 27646210 |
| near complete genome sequence of the animal feed probiotic, bacillus amyloliquefaciens h57. | bacillus amyloliquefaciens h57 is a bacterium isolated from lucerne for its ability to prevent feed spoilage. further interest developed when ruminants fed with h57-inoculated hay showed increased weight gain and nitrogen retention relative to controls, suggesting a probiotic effect. the near complete genome of h57 is ~3.96 mb comprising 16 contigs. within the genome there are 3,836 protein coding genes, an estimated sixteen rrna genes and 69 trna genes. h57 has the potential to synthesise four ... | 2016 | 27602182 |
| gene cloning and expression of cellulase of bacillus amyloliquefaciens isolated from the cecum of goose. | a kind of bacteria secreting cellulase and showing probiotic attributes was isolated from the cecum of goose and identified as bacillus amyloliquefaciens by analysis of 16s rrna gene sequence and named as b. amyloliquefaciens s1. in vitro assays, the enzymatic activity of the strain was determined by the reducing-sugar method, and the proper culture conditions of producing cellulase and some properties of the cellulase were investigated. the cultural mixture of the bacteria had a high cellulase ... | 2017 | 27575333 |
| anti-methicillin resistant staphylococcus aureus compound isolation from halophilic bacillus amyloliquefaciens mhb1 and determination of its mode of action using electron microscope and flow cytometry analysis. | the aim of this study was to purify, characterize and evaluate the antibacterial activity of bioactive compound against methicillin-resistant staphylococcus aureus (mrsa). the anti-mrsa compound was produced by a halophilic bacterial strain designated as mhb1. the mhb1 strain exhibited 99 % similarity to bacillus amyloliquefaciens based on 16s rrna gene analysis. the culture conditions of bacillus amyloliquefaciens mhb1 were optimized using nutritional and environmental parameters for enhanced a ... | 2016 | 27570306 |
| mutations in genes encoding antibiotic substances increase the synthesis of poly-γ-glutamic acid in bacillus amyloliquefaciens ll3. | poly-γ-glutamic acid (γ-pga) is an important natural biopolymer that is used widely in fields of foods, medicine, cosmetics, and agriculture. several b. amyloliquefaciens ll3 mutants were constructed to improve γ-pga synthesis via single or multiple marker-less in-frame deletions of four gene clusters (itu, bae, srf, and fen) encoding antibiotic substances. γ-pga synthesis by the δsrf mutant showed a slight increase (4.1 g/l) compared with that of the wild-type strain (3.3 g/l). the δituδsrf mut ... | 2017 | 27539744 |
| purification and characterization of a naringinase from a newly isolated strain of bacillus amyloliquefaciens 11568 suitable for the transformation of flavonoids. | an intracellular naringinase from bacillus amyloliquefaciens 11568 isolated from soil was purified, identified, and characterized. the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) analysis of the purified enzyme gave a single protein band corresponding to a molecular mass of 32kda. the optimum ph and temperature for naringinase and its α-l-rhamnosidase and β-d-glucosidase activities were ph 7.5 and 45°c, respectively. the enzymes were stable below 45°c between ph 3.5 and ... | 2017 | 27507445 |
| effects of mreb paralogs on poly-γ-glutamic acid synthesis and cell morphology in bacillus amyloliquefaciens. | actin-like mreb paralogs play important roles in cell shape maintenance, cell wall synthesis and the regulation of the d,l-endopeptidases, cwlo and lyte. the gram-positive bacteria, bacillus amyloliquefaciens ll3, is a poly-γ-glutamic acid (γ-pga) producing strain that contains three mreb paralogs: mreb, mbl and mrebh. in b. amyloliquefaciens, cwlo and lyte can degrade γ-pga. in this study, we aimed to test the hypothesis that modulating transcript levels of mreb paralogs would alter the synthes ... | 2016 | 27481703 |
| hypolipidemic, antiobesity and cardioprotective effects of fermented protein hydrolysates from sardinelle (sardinella aurita) in high-fat and fructose diet fed wistar rats. | the present study aims to evaluate the antiobesity, hypolipidemic and cardioprotective effects of fermented sardinelle (sardinella aurita) protein hydrolysates (fsphs) produced with two proteolytic bacteria, bacillus subtilis a26 (fsph-a26) and bacillus amyloliquefaciens an6 (fsph-an6). wistar rats were fed during 10weeks a standard laboratory diet, a high caloric diet (hcd) and a hcd coupled with the oral administration of sardinelle meat flour (smf) or fsphs. hcd caused hyperlipidemia and incr ... | 2016 | 27460865 |
| a complex inoculant of n2-fixing, p- and k-solubilizing bacteria from a purple soil improves the growth of kiwifruit (actinidia chinensis) plantlets. | limited information is available if plant growth promoting bacteria (pgpb) can promote the growth of fruit crops through improvements in soil fertility. this study aimed to evaluate the capacity of pgpb, identified by phenotypic and 16s rrna sequencing from a vegetable purple soil in chongqing, china, to increase soil nitrogen (n), phosphorus (p), and potassium (k) availability and growth of kiwifruit (actinidia chinensis). in doing so, three out of 17 bacterial isolates with a high capacity of ... | 2016 | 27445991 |
| inhibition of biofilm in bacillus amyloliquefaciens q-426 by diketopiperazines. | biofilm formation can make significant effects on bacteria habits and biological functions. in this study, diketopiperazines (dkps) produced by strain of bacillus amyloliquefaciens q-426 was found to inhibit biofilm formed in the gas-liquid interface. four kinds of dkps were extracted from b. amyloliquefaciens q-426, and we found that 0.04 mg ml(-1) dkps could obviously inhibit the biofilm formation of the strain. dkps produced by b. amyloliquefaciens q-426 made a reduction on extracellular poly ... | 2016 | 27430510 |
| [effects of interaction between vermicompost and probiotics on soil nronerty, yield and quality of tomato]. | in this study, we investigated the effects of two strains of probiotic bacteria (bacillus megaterium bm and bacillus amyloliquefaciens ba) combined with chemical fertilizers and vermicompost on the soil property, the yield and quality of tomato. the results showed that under the same nutrient level, vermicompost significantly increased the yield, soluble sugar and protein contents of fruit, the soil ph and available phosphorus when compared with chemical fertilizers. vermicompost combined with p ... | 2016 | 27396121 |
| phylogenetic distribution of extracellular guanyl-preferring ribonucleases renews taxonomic status of two bacillus strains. | the potential of microbial ribonucleases as promising antitumor and antiviral agents, determines today's directions of their study. one direction is connected with biodiversity of rnases. we have analyzed completed and drafted bacillus genomes deposited in genbank for the presence of coding regions similar to the gene of an extracellular guanyl-preferring rnase of bacillus amyloliquefaciens (barnase). orthologues of the barnase gene were detected in 9 species out of 83. all of these belong to "b ... | 2016 | 27373509 |
| characterization of a bacillus amyloliquefaciens strain for reduction of citrulline accumulation during soy sauce fermentation. | to reduce the amount of citrulline produced by arginine-consuming bacteria in the moromi mash during soy sauce production. | 2016 | 27323751 |
| biological control potential of bacillus amyloliquefaciens kb3 isolated from the feces of allomyrina dichotoma larvae. | most biocontrol agents for plant diseases have been isolated from sources such as soils and plants. as an alternative source, we examined the feces of tertiary larvae of the herbivorous rhino beetle, allomyrina dichotoma for presence of biocontrol-active microbes. the initial screen was performed to detect antifungal activity against two common fungal plant pathogens. the strain with strongest antifungal activity was identified as bacillus amyloliquefaciens kb3. the inhibitory activity of this s ... | 2016 | 27298603 |
| on the molecular structure of the amylopectin fraction isolated from "high-amylose" ae maize starches. | the amylopectin fractions from starch of a series of amylose-extender (ae) maize samples (hylon(®) v, vii and viii starches) were isolated and analysed for their molecular composition and structure. the fractions from all samples contained both a high and a low molecular weight fraction (hmf and lmf), of which lmf increased with the amylose content of the starch and appeared to have substantially more of long chains than hmf. a normal amylose-containing maize starch (nms), which served as a refe ... | 2016 | 27296443 |
| molecular characterization of alkaline protease of bacillus amyloliquefaciens sp1 involved in biocontrol of fusarium oxysporum. | an alkaline protease gene was amplified from genomic dna of bacillus amyloliquefaciens sp1 which was involved in effective biocontrol of fusarium oxysporum. we investigated the antagonistic capacity of protease of b. amyloliquifaciens sp1, under in vitro conditions. the 5.62 fold purified enzyme with specific activity of 607.69u/mg reported 24.14% growth inhibition of f. oxysporum. however, no antagonistic activity was found after addition of protease inhibitor i.e. pmsf (15mm) to purified enzym ... | 2016 | 27294522 |
| comparative proteomic analysis reveals intracellular targets for bacillomycin l to induce rhizoctonia solani kühn hyphal cell death. | bacillomycin l, a natural iturinic lipopeptide produced by bacillus amyloliquefaciens, is characterized by strong antifungal activity against a variety of agronomically important filamentous fungi including rhizoctonia solani kühn. to further understand its antifungal actions, proteomes were comparatively studied within r. solani hyphal cells treated with or without bacillomycin l. the results show that 39 proteins were alternatively expressed within cells in response to this lipopeptide, which ... | 2016 | 27267622 |
| functional role of bacteria from invasive phragmites australis in promotion of host growth. | we hypothesize that bacterial endophytes may enhance the competitiveness and invasiveness of phragmites australis. to evaluate this hypothesis, endophytic bacteria were isolated from p. australis. the majority of the shoot meristem isolates represent species from phyla firmicutes, proteobacteria, and actinobacteria. we chose one species from each phylum to characterize further and to conduct growth promotion experiments in phragmites. bacteria tested include bacillus amyloliquefaciens a9a, achro ... | 2016 | 27260154 |
| characterization of extracellular polymeric substances of bacillus amyloliquefaciens sqr9 induced by root exudates of cucumber. | bacillus amyloliquefaciens sqr9 is a plant growth-promoting rhizobacterium (pgprs) that forms biofilm on the roots of plants and protects them from a variety of pathogens. in this study, we reported the effect of root exudates produced by cucumber (cucumis sativus l.) at different developmental stages on the biochemical composition of the biofilm matrix of sqr9. the results showed that the amino acids present in the root exudates of cucumber were responsible for triggering biofilm formation of s ... | 2016 | 27254757 |
| tolerance induction of temperature and starvation with tricalcium phosphate on preservation and sporulation in bacillus amyloliquefaciens detected by flow cytometry. | the bacillus species have many applications in the preparation of various enzymes, probiotic, biofertilizer, and biomarkers for which the survival of resting cells and spore formation under different conditions are important. in this study, water and saline along with different mineral substances such as calcium carbonate, calcium phosphate, and silica were used for the detection of survival and preservation of bacillus amyloliquefaciens. the results showed intensive death of resting cells at 8 ... | 2016 | 27251036 |
| the crystal structure of the yknz extracellular domain of abc transporter yknwxyz from bacillus amyloliquefaciens. | bacillus possesses the peptide toxin sporulation-delaying protein (sdp), which can kill cells within a biofilm to support continued growth, thereby delaying the onset of biofilm sporulation. the four-component transporter yknwxyz acts as a major sdp efflux pump to protect cells against the endogenous sdp toxin, for which yknyz is a non-canonical atp-binding cassette (abc)-type transporter. yknyz consists of the following two components: (1) an individual protein (ykny) and (2) a respective perme ... | 2016 | 27243566 |
| isolation and characterization of a broad spectrum bacteriocin from bacillus amyloliquefaciens rx7. | we isolated a bacillus strain, rx7, with inhibitory activity against listeria monocytogenes from soil and identified it as bacillus amyloliquefaciens based on 16s rrna gene sequencing. the inhibitory activity was stable over a wide range of ph and was fully retained after 30 min at 80°c, after which it decreased gradually at higher temperatures. the activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-k, and trypsin, indicating its proteinaceous nature. this bacteriocin ... | 2016 | 27239477 |
| unraveling aspects of bacillus amyloliquefaciens mediated enhanced production of rice under biotic stress of rhizoctonia solani. | rhizoctonia solani is a necrotrophic fungi causing sheath blight in rice leading to substantial loss in yield. excessive and persistent use of preventive chemicals raises human health and environment safety concerns. as an alternative, use of biocontrol agents is highly recommended. in the present study, an abiotic stress tolerant, plant growth promoting rhizobacteria bacillus amyloliquefaciens (sn13) is demonstrated to act as a biocontrol agent and enhance immune response against r. solani in r ... | 2016 | 27200058 |
| a novel protein elicitor (peba1) from bacillus amyloliquefaciens nc6 induces systemic resistance in tobacco. | here we reported a novel protein elicitor from bacillus amyloliquefaciens nc6 induced systemic resistance (isr) in tobacco. the purification was executed by ion-exchange chromatography, native-page extraction and hplc, and the amino acid sequence was identified by mass spectrometry. this recombinant elicitor protein, expressed in escherichia coli by an e1 expression vector, had good thermal stability, and the elicitor caused a clearly defined hypersensitive response (hr) necrosis in tobacco leav ... | 2016 | 27194952 |
| the lipopeptide 6-2 produced by bacillus amyloliquefaciens anti-ca has potent activity against the biofilm-forming organisms. | both the whole cells and protoplasts of pseudomonas aeruginosa pao1 and bacillus cereus, two biofilm-forming bacteria, were disrupted by the lipopeptide 6-2 produced by bacillus amyloliquefaciens anti-ca. the lipopeptide 6-2 could also effectively inhibit the formation of biofilms and disperse pre-formed biofilms. live/dead staining of the biofilms grown in the absence or presence of the lipopeptide 6-2 showed that more dead bacterial cells in the presence of the lipopeptide than those in the ab ... | 2016 | 27184127 |
| effects of volatile organic compounds produced by bacillus amyloliquefaciens on the growth and virulence traits of tomato bacterial wilt pathogen ralstonia solanacearum. | the production of volatile organic compounds (vocs) by microbes is an important characteristic for their selection as biocontrol agents against plant pathogens. in this study, we identified the vocs produced by the biocontrol strain bacillus amyloliquefaciens t-5 and evaluated their impact on the growth and virulence traits of tomato bacterial wilt pathogen ralstonia solanacearum. the results showed that the vocs of strain t-5 significantly inhibited the growth of r. solanacearum in agar medium ... | 2016 | 27183998 |
| seed-borne endophytic bacillus amyloliquefaciens rwl-1 produces gibberellins and regulates endogenous phytohormones of oryza sativa. | some microorganisms are adapted to an endophytic mode, living symbiotically with plants through vertical transmission in seeds. the role of plant growth-promoting endophytes has been well studied, but those of seed-associated endophytic bacteria are less understood. the current study aimed to isolate and identify bacterial endophytes associated with rice (oryza sativa l. 'jin so mi') seeds, their potential to produce gibberellins (gas), and role in improving host-plant physiology. the isolated b ... | 2016 | 27182958 |
| biosynthesis of titanium dioxide nanoparticles using bacillus amyloliquefaciens culture and enhancement of its photocatalytic activity for the degradation of a sulfonated textile dye reactive red 31. | the present study aims at exploiting bacillus amyloliquefaciens for the biosynthesis of titanium dioxide nanoparticles and also investigates role of bacterial enzymes in the biosynthesis of titanium dioxide nanoparticles. bacterial synthesized as well as metal doped titanium dioxide nanoparticles were characterized by x-ray diffractometer (xrd), fourier transform infrared spectroscopy (ftir), transmission electron microscopy (tem), energy dispersive x-ray spectroscopy (edax). amylase activity (4 ... | 2016 | 27175828 |
| genome sequences of 12 spore-forming bacillus species, comprising bacillus coagulans, bacillus licheniformis, bacillus amyloliquefaciens, bacillus sporothermodurans, and bacillus vallismortis, isolated from foods. | here, we report the draft genomes of twelve isolates of five different bacillus species, all spore-forming, gram-positive bacteria. | 2016 | 27174261 |
| effects of microbial enzymes on starch and hemicellulose degradation in total mixed ration silages. | this study investigated the association of enzyme-producing microbes and their enzymes with starch and hemicellulose degradation during fermentation of total mixed ration (tmr) silage. | 2017 | 27165015 |
| biocontrol of geosmin-producing streptomyces spp. by two bacillus strains from chinese liquor. | streptomyces spp. producing geosmin have been regarded as the most frequent and serious microbial contamination causing earthy off-flavor in chinese liquor. it is therefore necessary to control the streptomyces community during liquor fermentation. biological control, using the native microbiota present in liquor making, appears to be a better solution than chemical methods. the objective of this study was to isolate native microbiota antagonistic toward streptomyces spp. and then to evaluate th ... | 2016 | 27161758 |
| bacillus nakamurai sp. nov., a black-pigment-producing strain. | two isolates of a gram-stain-positive, strictly aerobic, motile, rod-shaped, endospore-forming bacterium were identified during a survey of the bacillus diversity of the agriculture research service culture collection. these strains were originally isolated from soil and have a phenotype of producing a dark pigment on tryptic soy agar. phylogenetic analysis of the 16s rrna gene indicated that these strains were related most closely to bacillus subtilis subsp. inaquosorum (99.7 % similarity) and ... | 2016 | 27150918 |
| effects of fatty acids on the interfacial and solution behavior of mixed lipidic aggregates called solid lipid nanoparticles. | mutual miscibility of soylecithin, tristearin, fatty acids (fas), and curcumin was assessed by means of surface pressure-area isotherms at the air-solution interface in order to formulate modified solid lipid nanoparticles (sln). appearance of minima in the excess area (aex) and changes in free energy of mixing (∆g(0)ex) were recorded for systems with 20 mole% fas. modified slns, promising as topical drug delivery systems, were formulated using the lipids in combination with curcumin, stabilized ... | 2016 | 27150334 |
| influence of lipid composition, ph, and temperature on physicochemical properties of liposomes with curcumin as model drug. | the physicochemical properties of large unilamellar vesicles (luvs) were assessed with respect to lipid composition, ph, time, and temperature by monitoring their size, zeta potential, drug payload, and thermal behavior. a conventional thin film hydration technique was employed to prepare liposomes from soy phosphatidylcholine (spc), dipalmitoylphosphatidylcholine (dppc), dipalmitoylphosphatidylglycerol (dppg), and a 7:3 (m/m) mixture of dppc+dppg along with 30 mole% cholesterol in each combinat ... | 2016 | 27150333 |
| properties of an ionic liquid-tolerant bacillus amyloliquefaciens cmw1 and its extracellular protease. | an ionic liquid-tolerant bacterium, bacillus amyloliquefaciens cmw1, was isolated from a japanese fermented soybean paste. strain cmw1 grew in the presence of 10 % (v/v) 1-butyl-3-methylimidazolium chloride ([bmim]cl), a commonly used ionic liquid. additionally, strain cmw1 grew adequately in the presence of the hydrophilic ionic liquids 10 % (v/v) 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([emim]cf3so3) or 2.5 % (v/v) 1-butyl-3-methylimidazolium trifluoromethanesulfonate ([bmim]cf3s ... | 2016 | 27142029 |
| vegetative bacillus amyloliquefaciens cells do not confer protection against necrotic enteritis in broilers despite high antibacterial activity of its supernatant against clostridium perfringens in vitro. | in this study, the effect of bacillus amyloliquefaciens on clostridium perfringens was tested in vitro and in vivo. using an agar well diffusion assay, the inhibitory activity of b. amyloliquefaciens supernatant was analysed against a large collection of netb-positive and netb-negative c. perfringens strains. although strong growth inhibiting activity was detected against all c. perfringens isolates, it was significantly higher against virulent netb-positive c. perfringens strains compared with ... | 2016 | 27122203 |
| complete genome sequence of bacillus amyloliquefaciens b15 isolated from grape skin, a strain of strong inhibitory activity against fungi. | bacillus amyloliquefaciens b15 is a gram-positive, plant-associated bacterium which shows strong antifungal activity, isolated from grape skin in xinjiang, china. the genome of b. amyloliquefaciens b15 comprises a 4,006,754bp long circular chromosome containing 3991 protein coding genes and 109 rna genes. based on genomic analysis, we identified the giant gene clusters, nonribosomal peptidesynthetases (nrps), and polyketide synthases (pks), responsible for the biosynthesis of numerous bioactive ... | 2016 | 27114322 |
| characterization of innate immunity genes in the parasitic nematode brugia malayi. | the filarial nematode brugia malayi is one of the causative agents of lymphatic filariasis, a neglected tropical disease that affects 120 million people worldwide. the limited effectiveness of available anthelmintics and the absence of a vaccine have prompted extensive research on the interaction between brugia and its obligate bacterial endosymbiont, wolbachia. recent studies suggest that wolbachia is able to manipulate its nematode host immunity but relatively little is known about the immune ... | 2017 | 27110057 |
| effect of organic fertilizers prepared from organic waste materials on the production of antibacterial volatile organic compounds by two biocontrol bacillus amyloliquefaciens strains. | three organic fertilizers made of different animal and plant waste materials (bofs) were evaluated for their effects on the production of antibacterial volatile organic compounds (vocs) by two bacillus amyloliquefaciens strains sqr-9 and t-5 against the tomato wilt pathogen ralstonia solanacearum (rs). both strains could produce vocs that inhibited the growth and virulence traits of rs; however, in the presence of bofs, the production of antibacterial vocs was significantly increased. the maximu ... | 2016 | 27067079 |
| multiple effects of bacillus amyloliquefaciens volatile compounds: plant growth promotion and growth inhibition of phytopathogens. | biotic interactions through volatile organic compounds (voc) are frequent in nature. this investigation aimed to study the role of italic! bacillusvoc for the beneficial effects on plants observed as improved growth and pathogen control. four italic! bacillus amyloliquefacienssubsp. italic! plantarumstrains were screened for voc effects on italic! arabidopsis thalianacol-0 seedlings and italic! brassicafungal phytopathogens. voc from all four italic! bacillusstrains could promote growth of itali ... | 2016 | 27053756 |
| enhancement of fengycin production in bacillus amyloliquefaciens by genome shuffling and relative gene expression analysis using rt-pcr. | genome shuffling is an efficient approach for the rapid engineering of microbial strains with desirable industrial phenotypes. in this study, we used genome shuffling in an attempt to improve fengycin production of the wild-type strain bacillus amyloliquefaciens es-2-4. after 2 rounds of genome shuffling, a high-yield recombinant f2-72 (fmb72) strain that exhibited 8.30-fold increases in fengycin production was obtained. comparative analysis of synthetase gene (fena) expression was conducted bet ... | 2016 | 27035066 |
| obtaining a mutant of bacillus amyloliquefaciens xylanase a with improved catalytic activity by directed evolution. | this study aimed to obtain xylanase exhibiting improved enzyme properties to satisfy the requirements for industrial applications. the baxa gene encoding bacillus amyloliquefaciens xylanase a was mutated by error-prone touchdown pcr. the mutant, pcbaxa50, was screened from the mutant library by using the 96-well plate high-throughput screening method. sequence alignment revealed the identical mutation point s138t in xylanase (rebaxa50) produced by the pcbaxa50. the specific activity of the purif ... | 2016 | 26992794 |
| exploration of photocatalytic properties of microbially designed silver nanoparticles on victoria blue b. | victoria blue b (vbb) belongs to triphenylmethane group of dyes, which is widely used in textile industries. hence development of novel treatment methods are of considerable applications in its removal. in the current study, silver nanoparticles (agnps) formed by bacillus amyloliquefaciens was investigated for the degradation of vbb. interestingly, the uv-vis spectroscopy analysis of vbb-agnps treated samples showed a decrease in absorption at 615 nm, which is characteristic of pure vbb. this ti ... | 2016 | 26975321 |
| cyclic dipeptide cyclo(l-leucyl-l-prolyl) from marine bacillus amyloliquefaciens mitigates biofilm formation and virulence in listeria monocytogenes. | this study was intentionally focused on cyclo(l-leucyl-l-prolyl) (clp), a cyclic dipeptide with myriad pharmaceutical significance, to explore its antivirulence efficacy against the predominant foodborne pathogen,listeria monocytogenes(lm). minimum inhibitory concentration (mic) of clp against lm atcc 19111 was found to be 512 μg ml(-1) clp at sub-mics (64 128, 256 μg ml(-1)) demonstrated a profound non-bactericidal dose-dependent antibiofilm efficacy (on polystyrene and glass) against lm, which ... | 2016 | 26945590 |
| metabolite and mechanistic basis of antifungal property exhibited by endophytic bacillus amyloliquefaciens bmb 1. | plants are ubiquitously colonized by endophytic microorganisms which contribute significantly to plant health through production of plant growth regulators or disease suppression. in the present study, an endophytic bacterial isolate designated as bmb 1 with significant antifungal and plant growth promoting properties was isolated from the stem tissue of bacopa monnieri (l.) pennell. the isolate was studied in detail for the molecular and chemical basis of its bioactivity which proved it to have ... | 2016 | 26945577 |