| partial characterisation of the inhibitory substances produced by streptococcus oralis and related species. | the production of inhibitory substances was sought by deferred antagonism. streptococcus oralis, streptococcus mitis and streptococcus sanguis produced bactericidal concentrations of hydrogen peroxide, but no detectable bacteriocin. actinomycetes and neisseria pharyngis were particularly susceptible to the lethal action of hydrogen peroxide. streptococcus milleri produced bactericidal concentrations of hydrogen peroxide and bacteriocin-like substances. s. oralis strains were inhibited by the bac ... | 1988 | 3216798 |
| dangers of an uncomplicated tooth extraction. a case of streptococcus sanguis meningitis. | | 1988 | 3224048 |
| interaction between wild-type, mutant and revertant forms of the bacterium streptococcus sanguis and the bacterium actinobacillus actinomycetemcomitans in vitro and in the gnotobiotic rat. | in vitro, streptococcus sanguis inhibits the growth of actinobacillus actinomycetemcomitans, a presumed aetiological agent of localized juvenile periodontitis. when provided with glucose and good aeration, a growing culture of strep. sanguis was found to produce hydrogen peroxide at concentrations in excess of the maximum ld50 reported for strains of a. actinomycetemcomitans. this concentration of hydrogen peroxide also inhibits the growth of the producer organism. a mutant of strep. sanguis was ... | 1988 | 3228385 |
| [anaphylactoid purpura and streptococcus sanguis--culture medium of streptococcus sanguis induce c3c binding activity on vascular endothelial cells]. | | 1988 | 3230655 |
| interactions between plaque bacteria: final report on project. | | 1988 | 3245810 |
| [a clinical evaluation of sultamicillin fine granules in the treatment of meibomianitis]. | this study was performed to evaluate the clinical efficacy and the safety of sultamicillin (sbtpc) fine granules in the treatment of patients with meibomianitis. a dose of 375 mg sbtpc granules was orally given to 10 patients with meibomianitis after each meal, three times a day. the results obtained are summarized as follows. efficacies were rated as good in 7 cases, fair in 2 and poor in 1 with an efficacy ratio of 70.0%. no side effects were observed throughout the study. the organisms isolat ... | 1988 | 3249376 |
| a case of polymicrobial infective endocarditis involving neisseria mucosa occurring in an intravenous drug abuser. | the incidence of polymicrobial endocarditis has increased markedly in recent years, in association with the increasing level of abuse of intravenous drugs. neisseria mucosa, an upper respiratory tract commensal, is a rare cause of infective endocarditis. we report the first case of polymicrobial infective endocarditis involving neisseria mucosa occurring in an intravenous drug abuser. | 1988 | 3250412 |
| evidence for the existence of two classes of corncob (coaggregation) receptor in fusobacterium nucleatum. | | 1988 | 3254470 |
| isolation and characterization of a non-adherent mutant of streptococcus sanguis g9b. | | 1988 | 3254471 |
| effect of hemoglobin and of ferric ammonium citrate on the virulence of periodontopathic bacteria. | | 1988 | 3254476 |
| bacterial agglutinin activity in the saliva of human identical and fraternal twins. | the major factor in human saliva responsible for the specific aggregation of oral streptococci is a high molecular-weight glycoprotein (agglutinin). to determine if the level of this glycoprotein in whole and parotid saliva was genetically determined, agglutinin activity for streptococcus sanguis and streptococcus mutans in saliva obtained from identical and fraternal twins was compared. evidence for the heritability of agglutinin activity and also parotid flow rate and total protein was obtaine ... | 1988 | 3257085 |
| cell surface mutants of streptococcus sanguis with altered adherence properties. | | 1988 | 3268752 |
| [isolation and identification of bacteria lytic against streptococcus sanguis from dental plaque of young children]. | | 1988 | 3269906 |
| [the effect of air-powder abrasive instrument on prosthodontic materials]. | | 1988 | 3274139 |
| microbiological and immunological studies of adult periodontitis in patients with noninsulin-dependent diabetes mellitus. | the subgingival microflora and serum antibody response was examined in periodontitis patients with noninsulin-dependent diabetes mellitus (niddm), impaired glucose tolerance (igt), and normal glucose tolerance (ngt). the predominant cultivable microflora was determined for subgingival plaque sampled from two deep periodontal pockets in each of eight adult periodontitis patients with niddm. indirect immunofluorescence for bacteroides intermedius, bacteroides gingivalis, and haemophilus actinomyce ... | 1988 | 3276868 |
| isolation and structure of a new ansamycin antibiotic kanglemycin a from a nocardia. | | 1988 | 3281924 |
| [endocarditis prophylaxis with amoxicillin, clindamycin or erythromycin? serum bacteriostatic and bactericidal effect against streptococcus viridans]. | the effectiveness of antibiotic prophylaxis against endocarditis was assessed by testing the bactericidal and bacteriostatic action in serum of 12 healthy volunteers who had taken the recommended antibiotics according to laid down guidelines. blood was obtained from these subjects every two hours for 12 hours after oral intake of amoxicillin (3 g as a single dose), clindamycin (600 mg a single dose) or erythromycin (1.5 g plus another 0.5 g after six hours), the serum being tested against three ... | 1988 | 3292185 |
| cloning of the gene encoding streptococcal immunoglobulin a protease and its expression in escherichia coli. | we have identified and cloned a 6-kilobase-pair segment of chromosomal dna from streptococcus sanguis atcc 10556 that encodes immunoglobulin a (iga) protease activity when cloned into escherichia coli. the enzyme specified by the iga gene in plasmid pjg1 accumulates in the periplasm of e. coli mm294 cells and has a substrate specificity for human iga1 identical to that of native s. sanguis protease. hybridization experiments with probes from within the encoding dna showed no detectable homology ... | 1988 | 3294181 |
| the effect of adherent oral streptococci on the subsequent adherence of candida albicans to acrylic in vitro. | | 1987 | 3294954 |
| pyruvate oxidase in streptococcus sanguis under various growth conditions. | | 1987 | 3295684 |
| the effect of sucrose on the colonization of acrylic by candida albicans in pure and mixed culture in an artificial mouth. | the roles of sucrose and microbial interactions in the colonization of acrylic by candida albicans in an artificial mouth were investigated. the acrylic was colonized by pure cultures of the organism but a visible plaque was not produced whether or not sucrose was present. salivary bacteria had no effect in the absence of sucrose but when sucrose was present the numbers of c. albicans were significantly increased. it is suggested that this phenomenon is related to the production of extracellular ... | 1987 | 3301780 |
| effect of protease on cell surface structure, hydrophobicity and adhesion of tufted strains of streptococcus sanguis biotypes i and ii. | protease treatment of s. sanguis biotype i strains (aki and cr311) and s. sanguis biotype ii strains (cn3410 and psh1b) reduced cell surface hydrophobicity of all strains by up to 87.7% and reduced adhesion to saliva coated hydroxyapatite beads (s-sha) by up to 90%. all four strains carried lateral tufts of fibrils of two lengths on the cells. tuft fibrils and hydrophobicity were lost simultaneously from s. sanguis ii cn3410 when these properties were monitored simultaneously over 3 h. a rutheni ... | 1987 | 3302619 |
| a simplified diagnostic system for cultural detection and enumeration of streptococcus mutans. | a simple dip-slide test (cariescreen sm) based on msb selective agar was devised for detection and quantitation of streptococcus mutans in oral samples. for this test, a bacitracin tablet is dissolved in a vial containing buffered saline diluent. paraffin-stimulated saliva is collected in this diluent vial. a dip slide which incorporates a modified msb agar (minus bacitracin) is immersed briefly in the diluted saliva. after addition of a co2-generating tablet, the screw-cap dip slide is closed t ... | 1987 | 3305618 |
| microbial aspects of preventive regimes in patients with overdentures. | the aim of this study was to test effects of preventive regimes using fluoride and chlorhexidine to prevent caries and periodontal diseases in 34 patients with overdentures. the patients, who were treated with immediate overdentures, were distributed at random into three experimental groups. in these groups, different gels--a placebo, a fluoride, and a chlorhexidine-fluoride gel--were tested by daily application. supragingival plaque samples from selected surfaces of two abutment teeth were take ... | 1987 | 3305620 |
| the growth of oral bacteria on saliva. | the present experiments were aimed at studying the degradation of salivary glycoproteins by the oral microflora. to this end, s. sanguis i strain ny476 and s. sanguis ii (s. mitior) strain ny581 were grown continuously in human-whole saliva. under these conditions, the strains produced a variety of cell-associated hydrolytic activities, including glycosidases, exo- and endopeptidases, and esterases. s. sanguis ii generally exhibited higher levels of enzyme activity than did s. sanguis i, in part ... | 1987 | 3305628 |
| streptococcus mutans, lactobacilli and streptococcus sanguis in plaque from abutment teeth of cemented and of loose retainers. | | 1987 | 3308095 |
| causes and control of dental caries. | | 1987 | 3309652 |
| the effect of smokeless-tobacco extracts on the growth of oral bacteria of the genus streptococcus. | aqueous tobacco extracts were used to supplement a basic salts solution (bss) and a microbial medium. thin-layer chromatography revealed sucrose in only one of four extracts. discs saturated with extracts (0.1-50 mg/ml) failed to inhibit growth of any of the micro-organisms. supplementation (10 mg/ml) of bss with the tobaccos lacking sucrose resulted in augmented growth of streptococcus mutans, streptococcus salivarius and streptococcus sanguis whereas the sucrose-containing brand augmented only ... | 1987 | 3310979 |
| novobiocin-resistant mutants of streptococcus sanguis with reduced cell hydrophobicity and defective in coaggregation. | mutants of streptococcus sanguis resistant to novobiocin (novr-mutants) were isolated after mutagenesis of strain challis with ethyl methanesulphonate. the resistance phenotype was transferred by dna-mediated transformation back into the parent strain at high frequency suggesting resistance was due to mutation(s) in a single gene or in closely-linked genes. cells of novr-mutants had normal morphology and secreted similar proteins to the wild-type strain. however, mutant cultures had slower growt ... | 1987 | 3312487 |
| a simple method for determining extracellular polysaccharide-producing ability of oral streptococci. | a simple method is described for determining the types of extracellular polysaccharides produced by oral streptococci. the method yields quantitative results in which the amount of polysaccharides produced is normalised with respect to dna. results correlate well with previously published data for oral streptococci, except in the case of streptococcus oralis for which there is no previously published data. all species within the mutans group produced both soluble and insoluble glucan; while solu ... | 1987 | 3316938 |
| evaluation of evidence related to streptococci in the etiology of kawasaki disease. | | 1987 | 3321074 |
| investigations on the binding of erythrogenic toxin a of streptococcus pyogenes on human peripheral blood lymphocytes. i. light and electron microscopical demonstration of cell surface receptors using colloidal gold-labelled toxin. | receptors for erythrogenic toxin a (eta) of streptococcus pyogenes (strain ny-5) were demonstrated on human peripheral blood lymphocytes by binding of eta-gold conjugates to the cell surface. the specificity of the binding was proved in control experiments. the dark-red granules observed by light microscopy on unfixed cells were revealed by transmission electron microscopy to be patches of gold particles. by light microscopy on samples from 48 blood donors a mean value of 34 +/- 13% of eta-recep ... | 1987 | 3326374 |
| use of lytic bacteriophage for actinomyces viscosus t14v as a probe for cell surface components mediating intergeneric coaggregation. | a lytic bacteriophage for actinomyces viscosus t14v (the reference strain for actinomyces coaggregation group a) was isolated from raw sewage. this phage, designated bf307, also lysed the t14v-derived nonfimbriated mutant pk455-2 as well as a. viscosus mg-1 and t14av but not the other serotype 2 or serotype 1 strains of this species that were tested or any of nine actinomyces naeslundii isolates. phages bf307 belonged to bradley morphological group c and was similar in appearance to the a. visco ... | 1988 | 3335409 |
| streptococcus sanguis surface antigens and their interactions with saliva. | saliva-binding molecules of streptococcus sanguis and their receptors were investigated. streptococcal cell surfaces were extracted with a barbital buffer and examined immunochemically. strains g9b and blackburn, which adhere specifically to saliva-coated hydroxyapatite via immunologically related adhesins, possess 80-, 62-, and 52-kilodalton (kda), and 52-, 42-, and 29-kda polypeptides, respectively, which correlate with adhesion to saliva-coated hydroxyapatite. nonadherent strains adh- and m-5 ... | 1988 | 3335410 |
| effect of nutritional constraints on the biosynthesis of the components of the phosphoenolpyruvate: sugar phosphotransferase system in a fresh isolate of streptococcus mutans. | a procedure for the purification of enzyme i (ei) and the protein hpr, the general components of the phosphoenolpyruvate:sugar phosphotransferase system, from streptococcus mutans serotype c is presented. the method was also applied successfully to the purification of ei and hpr from streptococcus salivarius, streptococcus sobrinus, and streptococcus sanguis. using specific antibodies obtained against the proteins purified from s. mutans dr0001, we determined quantitatively by rocket electrophor ... | 1988 | 3338847 |
| response of a streptococcus sanguis strain to arginine-containing peptides. | for dental plaque organisms such as streptococcus sanguis, the ecological importance of the ability to utilize arginine as an energy source has been established in previous studies. the present investigation was undertaken to determine the ability of a strain of s. sanguis to process unsubstituted arginine-containing peptides. the organism was grown under glucose-limited conditions in a chemically defined medium, and peptide was added to washed, resting cells in a ph-stat at ph 7.0. filtrates ta ... | 1988 | 3343053 |
| cloning of a streptococcus sanguis adhesin which mediates binding to saliva-coated hydroxyapatite. | chromosomal dna from a salivary aggregating strain of streptococcus sanguis 12 was partially digested with psti and ligated into the plasmid vector puc18 and transformed into escherichia coli jm83. a total of 1,700 recombinant clones of e. coli were examined by a colony immunoassay with antisera raised against either s. sanguis 12 whole cells or s. sanguis 12 surface fibrils. five clones which reacted with one or the other antiserum were shown to be unique by western blotting (immunoblotting) an ... | 1988 | 3356463 |
| nitrate- and nitrite-reducing bacteria in the achlorhydric stomach. | the microbial composition of samples of gastric juice from eight achlorhydric patients was determined by aerobic and rigorously anaerobic culture techniques. bacteria from 16 genera were commonly isolated, but representatives of only three genera, (streptococci, neisseriae and haemophili) were isolated from every patient. nitrate and nitrite were both reduced by veillonellae, haemophili, staphylococci, corynebacteria, lactobacilli, flavobacteria and fusobacteria, but the potential rate of nitrat ... | 1988 | 3357192 |
| relatively penicillin-resistant streptococcus sanguis endocarditis in an adolescent. | | 1988 | 3357720 |
| a polysaccharide from streptococcus sanguis 34 that inhibits coaggregation of s. sanguis 34 with actinomyces viscosus t14v. | coaggregation between actinomyces viscosus t14v and streptococcus sanguis 34 depends on interaction of a lectin on a. viscosus t14v with a cell surface carbohydrate on s. sanguis 34. this carbohydrate was isolated, and its chemical makeup was established. the carbohydrate remained attached to s. sanguis 34 cells through extraction with triton x-100 and treatment with pronase. it was cleaved from the cell residue by autoclaving and purified by differential centrifugation and column chromatography ... | 1988 | 3360742 |
| predictors of endocarditis in isolates from cultures of blood following dental extractions in rats with periodontal disease. | rats with periodontitis and catheter-induced aortic valve vegetations underwent dental extractions. cultures of blood obtained 1 min later showed polymicrobial bacteremia in 19 of 19 rats, mostly due to viridans streptococci (18 of 19), morganella (15 of 19), group g streptococci (13 of 19), and staphylococcus aureus (10 of 19). viridans streptococci circulated in higher numbers than did group g streptococci and s. aureus (p less than .01). three days after dental extractions, 18 of 20 rats had ... | 1988 | 3361156 |
| acute vertebral osteomyelitis complicating streptococcus sanguis endocarditis. | the first well documented case of acute pyogenic vertebral osteomyelitis presenting as the initial manifestation of streptococcus sanguis endocarditis is reported. the importance of suspecting vertebral osteomyelitis in the presence of disc infection and the diagnostic value of imaging procedures are underlined. | 1988 | 3365031 |
| intergeneric rosettes: sequestered surface recognition among human periodontal bacteria. | the human oral bacteria, streptococcus sanguis and bacteroides loescheii, when mixed in equal numbers in vitro, formed large settling coaggregates. as the relative number of each cell type was changed, coaggregates became smaller until at cell-type ratios of 10 to 1, rosettes formed. rosettes consisting of a streptococcal cell in the center surrounded by bacteroides cells exhibited surface recognition properties of only the bacteroides, which coaggregated with many other cell types such as actin ... | 1988 | 3377498 |
| cloning and expression of a streptococcus sanguis surface antigen that interacts with a human salivary agglutinin. | human saliva contains a high-molecular-weight glycoprotein (agglutinin) which binds to specific streptococci in a calcium-dependent reaction leading to the formation of bacterial aggregates. we report the cloning of a gene encoding a surface antigen from streptococcus sanguis m5 and show that the expressed protein inhibits agglutinin-mediated aggregation and specifically binds the salivary agglutinin in a calcium-dependent fashion. clones isolated from the immunological screening of s. sanguis m ... | 1988 | 3410546 |
| variant streptococcus sanguis as an etiological agent of kawasaki disease. | | 1987 | 3423077 |
| the hydrophobicity of 'viridans' streptococci isolated from the human mouth. | the hydrophobicity of human oral streptococci was measured with the hexadecane assay modified by the incorporation of polyethylene glycol 6000. large variability in the hydrophobicity between cultures of some strains grown on different occasions was observed whereas other strains were less variable. the variation in hydrophobicity was significantly reduced by growing the cells in continuous culture in a chemostat under glucose-limiting conditions. the streptococcus mutans strains used all had lo ... | 1987 | 3436855 |
| failure of post-bacteraemia delayed antibiotic prophylaxis of experimental rabbit endocarditis. | twenty-one rabbits had a cannula placed in the left ventricle and 48 h later 1 ml of a culture of streptococcus sanguis nctc 7864 was injected into an ear vein. six hours later, 400 mg/kg of amoxycillin was administered intramuscularly to 17 animals, the others acting as non-antibiotic treated controls. two of the 17 rabbits died during the experimental period, and the cannula was incorrectly placed in another two. five of the remaining 13 animals developed endocarditis. all controls developed e ... | 1987 | 3440772 |
| adhesion of oral streptococci from a flowing suspension to uncoated and albumin-coated surfaces. | a flow cell system was developed which allowed the study of bacterial adhesion to solid substrata at well-defined shear rates. in addition, the system enabled the solid surfaces to be coated with a proteinaceous film under exactly the same shear conditions. in this flow cell system, adhesion of three strains of oral streptococci from a phosphate-buffered solution onto three different substrata was studied as a function of time in the absence and presence of a bovine serum albumin (bsa) coating a ... | 1987 | 3446750 |
| effects of highly concentrated stannous fluoride and chlorhexidine regimes on human dental plaque flora. | the aim of this study was to determine the effect of an intensive antimicrobial treatment on the number of streptococcus mutans, streptococcus sanguis, actinomyces viscosus/actinomyces naeslundii, and the total colony-forming units (cfu) in plaque. the dentition of human volunteers was treated in a dental office with either chlorhexidine (5%) or stannous fluoride (8%). following the office treatment with chlorhexidine, selected volunteers rinsed daily at home for seven or 49 days with chlorhexid ... | 1986 | 3455699 |
| characterization of human dental plaque formed on hydrogen-ion-sensitive field-effect transistor electrodes. | the purpose of this study was to evaluate (with scanning electron microscopy and microbiological characterization) the bacterial deposits which accumulate on hydrogen-ion-sensitive field-effect transistor electrodes (ph-isfet) under conditions normally employed for telemetric monitoring of changes in human dental plaque ph. electrodes were mounted in a carrier appliance which was worn for two, four, and six days. the plaque ph response to a sucrose solution increased with the age of the plaque, ... | 1986 | 3457051 |
| persistence and spread of the orally-implanted bacterium streptococcus sanguis between persons. | transformant strains of streptomycin-resistant streptococcus sanguis persisted 2.5 years after oral implantation in 6 out of 11 subjects. natural transmission of these bacteria to spouses had occurred in 5 of the 6 infected subjects. at follow-up examinations, the median value of streptomycin-resistant strep. sanguis per ml saliva was 226,000 c.f.u. in the infected subjects and 352,000 c.f.u. in their spouses. the median values of streptomycin-resistant strep. sanguis as a percentage of the tota ... | 1986 | 3458434 |
| enamel demineralization by acid produced from endogenous substrate in oral streptococci. | bovine enamel blocks were covered by a bacterial cell mass and held in the mouths of human volunteers by a palatal appliance, to study the effects of acid production from endogenous bacterial substrate on the ph of the mass and on enamel demineralization. variables were cells with and without endogenous substrate, rinsing with 10 per cent glucose or sucrose solution, or no rinsing and the type of organism. acid production from endogenous substrate by streptococcus mutans strain ib-1600, due, at ... | 1986 | 3459413 |
| secretory and serum antibody responses to oral bacterial antigens associated with recurrent aphthous ulceration. | | 1986 | 3459829 |
| the influence of diet on the growth of streptococcal bacteria on the molar teeth of monkeys (macaca fascicularis). | the rates of regrowth of bacteria in developmental grooves on these teeth were determined. plaque was removed from palatal grooves up to 96 h after cleaning and the number of bacteria and of individual streptococcal species were determined; streptococcus sanguis, streptococcus mutans and streptococcus mitior were the major ones. in monkeys fed one of three different diets, the numbers of each species reached stable levels 18-24 h after tooth cleaning. the median doubling times of the streptococc ... | 1986 | 3467667 |
| effect of monoclonal antibodies against lipoteichoic acid from the oral bacterium streptococcus mutans on its adhesion and plaque-accumulation in vitro. | five monoclonal antibodies directed against streptococcus mutans strain jbp lipoteichoic acid (lta) were characterized. they were all similarly reactive with the immunizing lta-containing extract, with intact strep. mutans jbp cells and with lta purified from lactobacillus casei. immobilized anti-lta antibodies removes lta from lta-containing extracts. the binding of antibodies to lta was inhibited by the aqueous extract but not by the organic extract of de-acylated lta, indicating reactivity wi ... | 1986 | 3467668 |
| characteristics of a hydrogen peroxide-forming pyruvate oxidase from streptococcus sanguis. | | 1987 | 3473418 |
| colonization of the human oral cavity by a streptococcus mutans mutant producing increased bacteriocin. | streptococcus mutans strain jh1005 is a mutant that produces levels of bacteriocin activity three-fold-elevated than those produced by its parent, jh1001. a single infection regimen with jh1005 was found to result in persistent colonization of the teeth of all three adult subjects tested. this is a significant improvement over jh1001, which required multiple exposures in order to colonize the teeth of humans reliably. the levels of total cultivable bacteria and indigenous s. sanguis were not aff ... | 1987 | 3476580 |
| relationship between dental plaque indices and bacteria in dental plaque and those in saliva. | a variety of indices has been developed for the quantitation of dental plaque. the aim of this study was to determine the correlation between the löe plaque index and the number of bacteria on the same tooth. furthermore, the effect of plaque accumulation on the salivary counts of some dental plaque organisms was estimated. twenty volunteers were asked to abstain from all oral hygiene for a one-week period. clinical indices and bacteriological samples were taken at the start and at the end of th ... | 1987 | 3476622 |
| isolation of bacteria lytic against streptococcus sanguis atcc 10558 from human dental plaque. | | 1987 | 3477320 |
| microbiology of the early colonization of human enamel and root surfaces in vivo. | this study describes the predominant early microflora on human teeth on the basis of microbiologic identification of 1742 fresh isolates. the isolates were obtained from four dental students who carried test pieces of enamel and root surface in the oral cavity for 4, 8, 12, and 24 h. during the experimental periods oral hygiene was discontinued. under equal conditions root surfaces were more heavily colonized than were enamel surfaces. however, the composition of the microbiota was the same. wit ... | 1987 | 3477852 |
| isolation, chemical and biological characterization of sulphated glycoproteins synthesized by rat buccal and palatal minor salivary glands in vivo and in vitro. | 35s-labelled sulphated glycoproteins (sgp) were isolated from these glands after the incorporation of radiosulphate in vivo and in vitro by fractionation of tissue and medium extracts on sepharose 4b and partial purification by deae-sephacel anion exchange chromatography. fractions were assessed for purity by sds-page and by cellulose-acetate electrophoresis. molecular weights ranged from 34,000 to 5 x 10(6). it was notable that the molecular size of sgp from the in vitro media was generally low ... | 1987 | 3479079 |
| lysis of streptococcus sanguis by an extracellular enzyme from the bacterium streptococcus mutans from human dental plaque. | the ability of crude extracellular enzyme produced by the oral bacterium streptococcus mutans al7-1 to lyse living cells of streptococcus sanguis atcc 10556, 10557 and 10558 was examined. this enzyme showed lytic activity of living cells and cell walls of only strep. sanguis atcc 10558 strain and severed at random the long chains of this strain of living cells. early log phase cells of this strain were more sensitive to this lytic enzyme than were late-log phase cells. in view of these results, ... | 1986 | 3479961 |
| effects of naf and snf2 on growth, acid and glucan production of several oral streptococci. | the effects of low concentrations of these fluorides on streptococcus sobrinus, streptococcus mutans, streptococcus salivarius, streptococcus mitior and streptococcus sanguis were investigated. without fluoride, mutans streptococci (strep. mutans and strep. sobrinus) produced more acid than the other strains; both fluorides reduced acid production in all strains. naf had little effect on growth; snf2 decreased growth in all strains, most evidently in the strep. mutans. in all growth conditions, ... | 1987 | 3482350 |
| bacterial peritonitis following endoscopic variceal sclerotherapy. | | 1987 | 3498663 |
| [studies on the adherence of oral streptococci on solid surface]. | | 1987 | 3505567 |
| chlorhexidine affects arginine metabolism as well as glycolysis in a strain of streptococcus sanguis. | | 1987 | 3507629 |
| safety and antibacterial properties of controlled release snf2. | two clinical studies were performed to evaluate the safety and efficacy of an intracoronal device for the controlled release of snf2. snf2-polycarboxylate cement, containing approximately 72 mg f-, was placed in subjects' molars requiring two-surface restorations. in the trial for safety, eight subjects had the restoration in place for 34 days. the salivary fluoride levels were elevated to a mean of 0.3/10(6) over the experimental period. urinary fluoride levels were only above baseline levels d ... | 1986 | 3511199 |
| effect of antimicrobial agents on root surface caries, alveolar bone loss, and microflora in rice rats. | two antimicrobial agents, 9-aminoacridine (0.2%) and minocycline (0.2%), were evaluated for their efficacy in inhibiting root surface caries, bone loss, and microflora in rice rats. a solution of 5000 ppm fluoride was used as a positive control for the inhibition of root surface caries, and double-distilled water was used as a negative control group. each rat was treated by having its molar teeth swabbed 2 x per day with the prescribed agent in its group for nine weeks. root caries reduction in ... | 1986 | 3517096 |
| differentiation of streptococcus sanguis and s. mitior by whole-cell rhamnose content and possession of arginine dihydrolase. | whole-cell rhamnose concentrations were measured in 48 strains of streptococci resembling streptococcus sanguis and s. mitior. physiological characteristics were tested by the api-20/strep system, and it was found that "typical" s. sanguis (arginine positive, aesculin positive) contained significant amounts of rhamnose, while "typical" s. mitior (arginine negative, aesculin negative) contained very low or undetectable amounts of rhamnose. both groups contained dextran-positive and dextran-negati ... | 1986 | 3517336 |
| gingivitis. | gingivitis is caused by substances derived from microbial plaque accumulating at or near the gingival sulcus; all other suspected local and systemic etiologic factors either enhance plaque accumulation or retention, or enhance the susceptibility of the gingival tissue to microbial attack. microbial species specifically associated with gingival health include streptococcus sanguis 1, s. d-7, and fusobacterium naviforme. bacteria involved in the etiology of gingivitis include specific species of s ... | 1986 | 3522644 |
| fibrinolytic therapy in bacterial endocarditis: experimental studies in dogs. | an investigation was undertaken to assess the value of adding streptokinase therapy to standard penicillin treatment of artificially induced infective endocarditis due to streptococcus sanguis. in pigs a low-grade infection could be induced which closely simulated streptococcal endocarditis in man, but for technical reasons this model proved unsatisfactory for further experimentation. in dogs the induction of s. sanguis endocarditis produced a more severe illness with a short natural history. do ... | 1986 | 3525170 |
| humoral responses and cross-reactivity to viridans streptococci in recurrent aphthous ulceration. | it has been proposed that recurrent aphthous ulceration (rau) is caused by hypersensitivity to oral streptococci. this hypothesis is based on reports that rau patients have increased levels of circulating igg antibodies against oral streptococci, and that rabbit antisera prepared against oral streptococci are cross-reactive with oral mucosa. using indirect immunofluorescence, we have investigated both of these reported phenomena. end-point titers of serum antibodies against three different strai ... | 1986 | 3525629 |
| construction of plasmid vectors for the detection of streptococcal promoters. | plasmid vectors have been constructed for detecting dna fragments that exhibit promoter activity in streptococcus sanguis. the plasmids are able to replicate in both s. sanguis and escherichia coli, and contain an erythromycin resistance marker which is expressed in both hosts. selection for promoter activity is dependent upon the insertion of appropriate dna fragments upstream from a promoterless chloramphenicol acetyl transferase gene (cat) from staphylococcus aureus. to facilitate this insert ... | 1986 | 3536665 |
| randomized prospective study of ceftazidime versus ceftazidime plus cephalothin in empiric treatment of febrile episodes in severely neutropenic patients. | in a prospective randomized study, ceftazidime monotherapy was compared with a combination of ceftazidime plus cephalothin in 102 febrile neutropenic patients. thirty bacteriologically documented infections, of which 23 were bacteremias, in 48 clinically assessable patients were treated with ceftazidime alone. twenty-four bacteriologically proven infections, of which 18 were bacteremias, in 42 clinically assessable patients were treated with a combination of ceftazidime and cephalothin. the clin ... | 1987 | 3551829 |
| characterization of lectinlike surface components on capnocytophaga ochracea atcc 33596 that mediate coaggregation with gram-positive oral bacteria. | the interactions between capnocytophaga ochracea atcc 33596 and streptococcus sanguis h1, actinomyces naeslundii pk984, or actinomyces israelii pk16 are dependent on specific recognitions between heat-sensitive adhesins on c. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfaces of these gram-positive coaggregation partners. the coaggregation of c. ochracea with each of these three organisms was inhibited by l-rhamnose and d-fucose and to a lesser exten ... | 1987 | 3570460 |
| characterization of a rat salivary sialoglycoprotein complex which agglutinates streptococcus mutans. | rat saliva agglutinated streptococcus mutans ingbritt and nctc 10449 and streptococcus sanguis nctc 7864 but not s. mutans nctc 10921, gs 5, or lm 7, streptococcus sobrinus 6715-13 or omz 65, or streptococcus cricetus hs 6, as measured turbidometrically. the specificity of agglutination by rat saliva was the same as that by human saliva. agglutination was associated with a mucin complex (rat salivary agglutinin complex [rs-a]) of sulfated sialoglycoproteins, with a trace of associated lipid and ... | 1987 | 3570462 |
| cell-to-cell interaction of streptococcus sanguis and propionibacterium acnes on saliva-coated hydroxyapatite. | cell-to-cell interaction (coaggregation) between propionibacterium acnes pk93 and streptococcus sanguis dl1 was measured on saliva-coated hydroxyapatite beads (sha) at bacterial concentrations between 1.3 x 10(6) and 6.7 x 10(8) cells per ml. four hundredfold more dl1 than pk93 cells adhered to the saliva-coated beads, and the adherence of s. sanguis was proportional to cell input. sha precoated with 3 x 10(8) dl1 cells bound 75 to 80% of available pk93 cells at all input amounts tested, up to a ... | 1987 | 3570474 |
| streptococcus sanguis ii (viridans) prosthetic valve endocarditis with myocardial, splenic and cerebral abscesses. | | 1987 | 3577142 |
| [purification and characterization of a new amphipathic antigen from streptococcus sanguis atcc 10557]. | | 1987 | 3584404 |
| fatal multivalvular endocarditis due to streptococcus milleri and streptococcus sanguis. | | 1987 | 3595587 |
| role of sialic acid in the kinetics of streptococcus sanguis adhesion to artificial pellicle. | evaluation of the kinetics of adhesion of streptococcus sanguis 10556 to saliva-coated hydroxylapatite revealed that sialic acid played a role in the formation of a stable cell-substratum complex. in a previous paper (m. m. cowan, k. g. taylor, and r. j. doyle, j. dent. res. 65:1278-1283, 1986) the adhesion was found to take place in two distinct stages: a reversible equilibrium, probably governed by long-range forces, followed by a transition to higher-affinity binding. in the present study, ar ... | 1987 | 3596799 |
| differential medium for mixed cultures of alpha-hemolytic streptococci from blood. | alpha-hemolytic streptococci (ahs) were isolated from blood cultures from 100 patients, and species were identified by the ruoff and kunz scheme. when isolates were inoculated onto sheep blood agar, all 100 cultures appeared to be pure, with identifications based on colonial morphology and gram stain. when isolates were subcultured onto mitis salivarius agar (msa), mixtures of two species of ahs were detected in 10 cultures from patients (10%). these mixed cultures would have been reported as pu ... | 1987 | 3597746 |
| responses of platelets to strains of streptococcus sanguis: findings in healthy subjects, bernard-soulier, glanzmann's, and collagen-unresponsive patients. | the ability of endocarditis and dental strains of streptococcus sanguis to induce platelet aggregation in plasma (prp) from normal subjects were examined and compared to responses of prp with known platelet membrane glycoprotein (gp) and response defects. s. sanguis strains differed in their ability to induce normal prps to aggregate. strains that induced prp aggregation in more than 60% of donors were significantly faster agonists (mean lag times to onset of aggregation less than 6 min) than th ... | 1987 | 3603413 |
| septic discitis as a presentation of endocarditis. | | 1987 | 3626915 |
| structure of a new hexasaccharide from the coaggregation polysaccharide of streptococcus sanguis 34. | the major constituent of a coaggregation polysaccharide from streptococcus sanguis 34 is a hexasaccharide, isolated as the alditol. the proposed structure is alpha-d-galpnac-(1----3)-beta-l-rhap-(1----4)-beta-d-glcp-(1----6) -beta-d-galf- (1----6)-beta-d-galpnac-(1----3)-d-galol, based upon g.l.c.-m.s. of alditol acetates and partially methylated alditol acetates, f.a.b.-m.s., 1h-n.m.r. spectroscopy, g.l.c.-m.s. of trimethylsilylated (+)- and (-)-2-butyl glycosides, and cleavage by alpha-n-acety ... | 1987 | 3652075 |
| the utility of osler's nodes in the diagnosis of infective endocarditis. | the etiology of osler's nodes remains controversial. we describe a patient with endocarditis in whom streptococcus sanguis was grown from the biopsy of such a lesion. bacterial embolic events are likely to be the major pathogenetic event in the development of osler's nodes, and recognition of the etiology of infective endocarditis may be found through biopsy of them. | 1987 | 3652766 |
| enzymatic modification of glycocalyx in the treatment of experimental endocarditis due to viridans streptococci. | the presence of abundant surface polysaccharide, or glycocalyx, on viridans streptococci has been associated with failure to eradicate the organism from experimental cardiac vegetations during penicillin treatment. the role of glycocalyx in retarding sterilization was tested by in vivo administration of dextranase, an endohydrolase that attacks internally situated alpha (1-6) linkages. dextranase and penicillin, either singly or in combination, were used to treat experimental endocarditis. after ... | 1987 | 3655403 |
| aggregation of streptococcus sanguis biotypes i and ii by parotid saliva: a comparison between peritrichously fibrillar and tufted strains. | twelve strains of streptococcus sanguis biotype i and seven strains of streptococcus sanguis biotype ii carrying either peritrichous fibrils or tufts of fibrils, were examined for their susceptibility to aggregation by parotid saliva. salivary aggregation was evaluated using a spectrophotometric measurement of sedimentation to assess clump size. a clear distinction emerged between structural sub-groups. irrespective of biotype, strains carrying peritrichous fibrils aggregated strongly whilst tuf ... | 1987 | 3657600 |
| genetic relationships among the oral streptococci. | genetic relationships and species limits among the oral streptococci were determined by an analysis of electrophoretically demonstrable variation in 16 metabolic enzymes. fifty isolates represented 40 electrophoretic types, among which the mean genetic diversity per locus was 0.857. mannitol-1-phosphate dehydrogenase was not detected in isolates of the sanguis species complex, and glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were absent in species of the mutans complex. ... | 1987 | 3667531 |
| [infectious endocarditis in a patient with osteogenesis imperfecta]. | | 1987 | 3669792 |
| the isolation of cross-reactive monoclonal antibodies: hybridomas to streptococcal antigens cross-reactive with mammalian basement membrane. | based upon the assumption that post-streptococcal sequelae are the result of cross-reactive antibodies, hybridomas were prepared from the spleens of mice immunized with group a type 12 streptococcal cell membranes (scm) specifically to screen for such cross-reactive antibodies. one fusion produced a cell population displaying antibodies reactive to both scm and glomerular basement membrane (gbm) antigens as demonstrated by elisa technique. ascites produced by this cell population also showed rea ... | 1987 | 3679253 |
| one and two doses of cephradine in the prophylaxis of experimental streptococcal endocarditis. | the efficacy of cephradine in the prophylaxis of rabbit streptococcus sanguis endocarditis was investigated. three days after cardiac catheterization and prior to challenge with s. sanguis, rabbits received either 1000 mg/kg (ten animals) or 500 mg/kg cephradine intramuscularly. infective endocarditis was prevented in only 30% of the animals. the addition of a second dose of cephradine (100 mg/kg) 8 h after an initial dose of 400 mg/kg did not prevent streptococcal endocarditis in 80% of animals ... | 1987 | 3680090 |
| environmental ph as a factor in the competition between strains of the oral streptococci streptococcus mutans, s. sanguis, and "s. mitior" growing in continuous culture. | strains of streptococcus mutans (biotype 1), streptococcus sanguis, and streptococcus mitior have been grown in mixed continuous culture in a semidefined medium under glucose limitation at a growth rate of d = 0.1 h-1. the effect of varying the environmental ph on the proportions of the different populations within the community has been determined. initially the populations were allowed to reach steady state at ph 7.0 when s. sanguis was dominant with s. mutans and "s. mitior" maintaining simil ... | 1987 | 3690424 |
| variable colonization by oral streptococci in molar fissures of monoinfected gnotobiotic rats. | germfree sprague-dawley rats, fed a high-sucrose diet, were monoinfected with strains of streptococcus mitis, streptococcus sanguis, and streptococcus mutans. viable cell recoveries from six molar teeth, considered to reflect mainly bacterial colonization of intact fissures, were in the order of 10(6), 10(7), and 10(8) cfu, respectively. some of the implications of the variation of bacterial plaque-forming ability in the rat model are discussed. | 1986 | 3699897 |
| use of the photoaffinity cross-linking agent n-hydroxysuccinimidyl-4-azidosalicylic acid to characterize salivary-glycoprotein-bacterial interactions. | the present study has utilized the iodinatable cross-linking agent n-hydroxysuccinimidyl-4-azidosalicylic acid (asa) to examine the specific interaction between the proline-rich glycoprotein (prg) of human parotid saliva and streptococcus sanguis g9b. the binding of 125i-asa-prg to streptococcus sanguis g9b displayed saturation kinetics, reversibility and was inhibited by unlabelled prg. inhibition studies with other glycoproteins and saccharides indicated that binding was mediated by a bacteria ... | 1986 | 3707547 |
| high-performance liquid chromatographic analysis of bacterial fatty acid composition for chemotaxonomic characterization of oral streptococci. | a high-performance liquid chromatographic method was developed to analyze the fatty acid composition of bacterial lipids. after saponification of lipids extracted from bacteria, the liberated fatty acids were labeled with a fluorescence reagent, 4-bromomethyl-7-acetoxycoumarin, followed by reversed-phase high-performance liquid chromatographic separation and fluorescence detection. all bacterial fatty acids were simultaneously separated within 30 min and sensitively determined. this method was a ... | 1986 | 3722368 |
| comparative capacity of orally administered amoxicillin and parenterally administered penicillin-streptomycin to protect rabbits against experimentally induced streptococcal endocarditis. | a single-intramuscular-dose immunization regimen with a penicillin g-streptomycin combination was compared with three oral-dose amoxicillin regimens for the capacity to prevent streptococcus sanguis infections of experimentally induced valvular heart lesions in rabbits. challenge doses of 10(4), 10(6), and 10(8) cfu of a strain of s. sanguis equally susceptible to penicillin and amoxicillin were used in this study. measured by recovery of test organisms from endocardial lesions, the lowest conce ... | 1986 | 3729348 |
| kinetics of adhesion of the oral bacterium streptococcus sanguis ch3 to polymers with different surface free energies. | the kinetics of adhesion of streptococcus sanguis ch3 from suspension to polymers with different surface free energies were studied by using three bacterial concentrations (2.5 x 10(7), 2.5 x 10(8), and 2.5 x 10(9) cells per ml-1). substratum surface free energies (gamma s) ranged from 18 to 120 erg cm-2. the kinetics of bacterial adhesion to these surfaces showed a typical two-step adhesion process, indicating an equilibrium in both steps. in the initial adhesion step (step 1), low equilibrium ... | 1986 | 3729392 |
| preparation of a sialic acid-binding protein from streptococcus mitis ks32ar. | a recent report has identified a lectin on the surfaces of several strains of streptococcus mitis and streptococcus sanguis with specificity for an n-acetylneuraminic acid alpha 2,3-galactose-beta 1,3-n-acetylgalactosamine sequence (p.a. murray, m.j. levine, l.a. tabak, and m.s. reddy, biochem. biophys. res. commun. 106:390-396, 1982). in the present study, purification and characterization of this sialic acid-binding protein (sabp) was begun. a clinical isolate of s. mitis was grown to mid stat ... | 1986 | 3733221 |
| graft infection and bacteremia with a tolerant l-form of streptococcus sanguis in a patient receiving hemodialysis. | i report a case of a tolerant l-form streptococcus sanguis infection involving an artificial vascular access site that was probably acquired from a nonhuman source; this is the first report of such an infection in a human. gram stains from the infected access site and blood cultures revealed an aberrant form which could only be recovered by passage through hypertonic sucrose media before being reisolated and subsequently identified as s. sanguis. the same organism was isolated from a pet dog of ... | 1986 | 3745426 |