| an in vitro model to study bacterial invasion of periodontal tissues. | in periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. to study these mechanisms in greater detail, we used the pf hr-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. three gram-negative anaerobic periodontopathic organisms, bacteroides gingivalis, fusobacterium nucle ... | 1988 | 3422291 |
| tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells. | when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ... | 1987 | 3440028 |
| infectious and sexually transmitted diseases: implications for dental public health. | the acquired immunodeficiency syndrome (aids) has stimulated renewed attention toward infectious diseases and dental public health. currently, aids is defined as individuals with kaposi's sarcoma and/or pneumocystis carinii pneumonia and/or other life-threatening opportunistic infections (e.g., specific forms of tuberculosis or meningitis, candidal esophagitis), and associated immunosuppression that cannot be accounted for by another disease process and/or medications. as of january 1986, the ai ... | 1986 | 3457189 |
| desulfuration of cysteine and methionine by fusobacterium nucleatum. | fusobacterium nucleatum is a gram-negative anaerobic rod-shaped bacterium frequently isolated from human dental plaque. it is capable of the desulfuration of cysteine and methionine, resulting in the formation of sulfide and thiol volatiles, respectively. intact cells, as well as cell-free extracts produced by french pressure cell lysis of f. nucleatum, hydrolyzed radiolabeled cysteine to produce sulfide, pyruvic acid, and ammonia. the hydrolysis products of radiolabeled methionine were a volati ... | 1986 | 3458742 |
| frequency distributions of periodontal attachment loss. clinical and microbiological features. | the present investigation attempted to determine if the pattern of past periodontal destruction could be concisely summarized, and related to other clinical and microbiological parameters. 61 subjects between the ages of 12 and 61 years with destructive periodontal disease were evaluated at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. the frequency distribution of baseline attachment level measurements was computed for each individu ... | 1986 | 3462210 |
| ethology in animal quarters. | this contribution will be concerned with the interaction between environment, adaptability optimization and behaviour. animal laboratory experiments demand repeated measurements under identical environmental conditions. this is a prerequisite for the conventional statistical methodology used in order to clarify causal relationships involved in various biological functions. the understanding of biological functions is a necessary fundament for knowledge to prevent illness and to achieve a palliat ... | 1986 | 3469882 |
| certain bacterial species and morphotypes in localized juvenile periodontitis and in matched controls. | subgingival microflora in localized juvenile periodontitis (ljp) was examined by culture of certain bacterial species and by direct dark-field microscopy in affected and unaffected periodontal pockets of 11 patients and in their age- and sex-matched controls. only actinobacillus actinomycetemcomitans (a.a.) correlated to periodontal destruction and occurred most frequently at the affected ljp sites. a.a., bacteroides intermedius and fusobacterium nucleatum were isolated in proportions greater th ... | 1987 | 3473219 |
| a clinical, demographic and microbiologic study of anug patients in an urban dental school. | 35 anug patients were examined and compared clinically and demographically. plaque removed from ulcerated sites in 20 patients was cultured using quantitative anaerobic procedures and examined by electron and darkfield microscopy. patients were classified as having anug when presenting with ulceration and necrosis of interproximal papillae, pain and bleeding. the clinical symptoms of fetid odor, pseudomembrane formation, lymphadenopathy and elevated body temperature were present in 97%, 85%, 61% ... | 1987 | 3476504 |
| effect of periodontal therapy on specific antibody responses to suspected periodontopathogens. | the effects of clinically successful periodontal therapy were studied in juvenile periodontitis (jp) and rapidly progressive periodontitis (rp) patients and compared with periodontally healthy subjects (hs). serum samples were obtained in 35 hs prior to the study and in 12 of these subjects 3-4 years later. serum samples were obtained from 50 jp patients initially, 9 subjects immediately following surgical therapy and 29 of these subjects 3-4 years later. rp patients provided 46 initial serum sa ... | 1987 | 3476519 |
| structural determinants of activity of chlorhexidine and alkyl bisbiguanides against the human oral flora. | we assayed chlorhexidine and a series of its analogues, in which the chlorophenyl terminal substituents were replaced with alkyl chains, for their in vitro antimicrobial activity against the gram-negative and gram-positive oral bacteria. changes in antimicrobial activity were correlated with changes in agent structure for identification of structural criteria which may be important in the optimization of agent activity. chlorhexidine showed substantial antimicrobial activity against the gram-neg ... | 1987 | 3476581 |
| 4 beta-pdbu contracts parenchymal strip and synergizes with raised cytosolic calcium. | the effect of various concentrations of 4 beta-phorbol dibutyrate, (4-beta pdbu) phorbol myristate acetate (pma), and 4 beta-phorbol didecanoate (4 beta-pdd) were studied on the guinea-pig parenchymal strip. the order of potency, 4 beta-pdbu greater than pma greater than 4 beta-pdd, was the inverse of their lipid solubility. 4 beta-pdbu, 10(-9)-10(-4) m, caused a powerful, slow, sustained contraction starting within 2-3 min and reaching maximum in approximately 45 min, the maximum being 170% of ... | 1987 | 3478200 |
| distribution of alpha-amylase activity in selected broiler tissues. | in an examination of broiler alpha-amylase, significant variation in the serum enzyme activity level was noted, adult levels were lower than those of young chicks. analysis of alpha-amylase activity in various body fluids and tissues of 11-day and 7-week-old broilers indicated that the liver cannot be considered a source of alpha-amylase, although there was activity in both liver tissue and bile of 10 units/g wet weight and 35 units/100 ml, respectively. fluid from the oral cavity had low levels ... | 1986 | 3486408 |
| mucosal induction of systemic t cell tolerance by fusobacterium nucleatum. | the present study was undertaken to determine if mucosal presentation of the periodontopathic bacterium fusobacterium nucleatum could induce systemic tolerance. two separate protocols of mucosal priming were carried out. in the first, mice were gastrically intubated on 2 consecutive days; this was repeated 5 days later. in the second protocol, mice were similarly primed but received another priming dose after a further 7 days. positive control mice were similarly primed with sheep red blood cell ... | 1986 | 3488389 |
| extensive proliferation of mature connective-tissue type mast cells in vitro. | there are two phenotypically distinct subpopulations of mast cells in rodents: connective tissue-type mast cells (ctmc) and mucosal mast cells (mmc). these populations differ in their location, cell size, staining characteristics, ultrastructure, mediator content and t-cell dependency. several investigators recently reported a further subclass of mast cells which arise when normal mouse haematopoietic cells are cultured with interleukin-3 (il-3); il-3 is an activity similar or identical to mast- ... | 1986 | 3491321 |
| factors affecting antimicrobial susceptibility of fusobacterium species. | fifteen clinical isolates of fusobacterium species were studied to determine their quality of growth on five agar media, their susceptibility to penicillin, cephalothin, cefoxitin, and cefotaxime, the inoculum effect, and the presence of l forms and beta-lactamase. wilkins-chalgren agar supported confluent growth best, but fusobacterium nucleatum exhibited poor growth on all agar media. most isolates exhibited poor reproducibility of mic results with repeated agar dilution testing. however, most ... | 1987 | 3494743 |
| study of the growth factors for the mammary gland: epidermal growth factor and mesenchyme-derived growth factor. | the physiological importance of egf in the development of the mouse mammary gland during pregnancy and in spontaneous mammary tumorigenesis has been documented by a series of experimental results presented herein. in our study, we have taken a variety of experimental approaches including radioimmunoassay of egf in the submandibular gland and plasma, mammary gland organ/cell culture, egf receptor assay, sialoadenectomy and treatment with egf and egf antibodies to assess the role of egf in the mam ... | 1987 | 3498710 |
| intrathoracic mycotic aneurysm detected by indium-111 labelled autologous neutrophils with single photon emission computed tomography. | | 1987 | 3499001 |
| activity of a partially purified human bcgf on murine assays for b-cell stimulatory factors. i. bcgf ii-like activity of human bcgf. | to further characterize a human b-cell growth factor (bcgf) produced by phytohemagglutinin (pha) p-stimulated peripheral blood t cells, a partially purified preparation of this material was tested in a number of murine assays for b-cell stimulatory factors (bsf). human bcgf lacked murine bsf-1 activity as assessed via the induction of polyclonal proliferation of anti-igm-stimulated murine b cells; however, this material consistently augmented the proliferative response of murine b cells to anti- ... | 1987 | 3499989 |
| effect of treatment with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine/mptp/on mouse substantia nigra in vitro. fluorescence and electron microscopic examination. | | 1987 | 3501590 |
| a penicillinase-producing fusobacterium nucleatum strain isolated from blood culture. | | 1987 | 3501981 |
| comparative gene mapping: a valuable new tool for mammalian developmental studies. | technological advances in the 1970s encouraged the mapping of homologous gene loci in different mammalian species, including mouse and man. one hundred eighty-five homologous loci have now been mapped in these two species. conservation of linkage is sufficient to identify substantial segments of the two genomes that have been left intact since their divergence from a common ancestor. the recognition of these conserved segments allows experimental manipulation of mouse chromosomes or chromosomal ... | 1987 | 3502975 |
| [adherence of fusobacterium nucleatum to human cells]. | | 1987 | 3505564 |
| dna reactivity and in vitro cytotoxicity of the novel antitumor agent 1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (nsc-348948) in human embryo cells. | 1,5,2,4-dioxadithiepane-2,2,4,4-tetraoxide (cyclic-soso) is structurally a novel synthetic compound but may functionally act as an alkylating agent. the effects of cyclic-soso on dna were studied in imr-90 and va-13 human embryo cells by means of dna alkaline elution analysis. in contrast to a number of bifunctional alkylating agents, cyclic-soso produced no dna-dna interstrand cross-links in either cell line, even at concentration which produced a greater than 3 log cell kill. at equimolar conc ... | 1986 | 3509975 |
| a borrelia-specific monoclonal antibody binds to a flagellar epitope. | in immunofluorescence assays monoclonal antibody h9724 recognized eight species of the spirochetal genus borrelia but not representatives of the genera treponema, leptospira, and spirochaeta. we examined the reactivity of h9724 against subcellular components of borrelia hermsii, an agent of relapsing fever, and b. burgdorferi, the cause of lyme disease. h9724 bound to isolated periplasmic flagella of the two borreliae. in western blots the antibody reacted with the predominant protein in flagell ... | 1986 | 3516878 |
| [nosocomial infections caused by klebsiella pneumoniae in a neonatal unit and a unit for low-birth-weight infants]. | | 1986 | 3518962 |
| attachment level changes in destructive periodontal diseases. | the present communication attempts to summarize some of the features of attachment loss which are of interest to the clinician and the statistician analyzing data from clinical trials. these include the measurements employed to detect changes in attachment level, the nature of the destructive disease process and the effects of therapy on the attachment level measurements. although there are several difficulties associated with the attachment level measurement, at the present time it appears to b ... | 1986 | 3522651 |
| [monoclonal antibodies to the antigens of human hela tumor cells]. | a panel of 6 hybridomas "xejima" producing monoclonal antibodies specific to hela cells is prepared. monoclonal antibodies do not bind to antigens of human diploid fibroblasts, human continuous b- and t-lymphocytes and animal cell lines. the specificity of monoclonal antibodies to cellular antigens of 5 hela-like cell lines and 6 human tumour cells lines, not contaminated with hela cells, is determined. antibody containing ascitic fluid and culture media of hybridomas xejima-3, -12, -13, and -22 ... | 1986 | 3526672 |
| [monoclonal antibodies to the antigens of human hela tumor cells]. | a panel of 6 hybridomas "xejima" producing monoclonal antibodies specific to hela cells is prepared. monoclonal antibodies do not bind to antigens of human diploid fibroblasts, human continuous b- and t-lymphocytes and animal cell lines. the specificity of monoclonal antibodies to cellular antigens of 5 hela-like cell lines and 6 human tumour cells lines, not contaminated with hela cells, is determined. antibody containing ascitic fluid and culture media of hybridomas xejima-3, -12, -13, and -22 ... | 1986 | 3526672 |
| [sensitivity of anaerobes to 8 antibiotics]. | we studied the susceptibility of 282 obligate anaerobes to 8 antibiotics. the strains were isolated from clinical specimens taken during 1983 and 1984. minimal inhibitory concentrations (mics) were determined using a microplate method for 245 strains and agar dilution for 33 bacteroides asaccharolyticus strains. tested strains were as follows: 85 bacteroides fragilis, 71 clostridium, 19 fusobacterium nucleatum, 35 peptostreptococcus, 39 bacteroides oralis and bacteroides bivius and 33 bacteroide ... | 1986 | 3534760 |
| [origin, course and recession of an infectious disease, melioidosis, in temperate countries]. | | 1986 | 3535711 |
| induction of bacterial mutations by aminopyrazoles, compounds which cause mammary cancer in rats. | an aminopyrazole pd 71627 (5-amino-1,3-dimethyl-1h-pyrazol-4-yl) (2-fluorophenyl)methanone, and two amide derivatives, pd 108298, n-[4-(2-fluorobenzoyl)-1,3-dimethyl-1h-pyrazol-5-yl]-2- ([3-(2-methyl-1- piperidinyl)-propyl]amino) acetamide-(z)-2-butanedioate (1:2), and pd 109394, 2-(diethylamino)-n-[4-(2- fluorobenzoyl)-1,3-dimethyl-1h-pyrazol-5-yl]acetamide hydrochloride, proposed neuroleptic drugs, were found to elicit mammary adenocarcinomas in male rats after 13 weeks of treatment. these com ... | 1986 | 3536141 |
| immunohistochemical study of the distribution of vasotocin reacting neurons in avian diencephalon. | vasotocin (vt)- and neurophysin (nph)-containing neurons were immunocytochemically demonstrated in the diencephalon of the japanese quail (coturnix coturnix japonica), domestic fowl (gallus gallus), and pekin duck (anas platyrhynchos). in these three avian species, the immunoreactive cells are similarly distributed into three different diencephalic regions: lateral (l), periventricular (p), and dorsal diencephalic (dd). a different number of cell clusters can be easily identified in each region ... | 1986 | 3540108 |
| immunohistochemical study of the distribution of vasotocin reacting neurons in avian diencephalon. | vasotocin (vt)- and neurophysin (nph)-containing neurons were immunocytochemically demonstrated in the diencephalon of the japanese quail (coturnix coturnix japonica), domestic fowl (gallus gallus), and pekin duck (anas platyrhynchos). in these three avian species, the immunoreactive cells are similarly distributed into three different diencephalic regions: lateral (l), periventricular (p), and dorsal diencephalic (dd). a different number of cell clusters can be easily identified in each region ... | 1986 | 3540108 |
| mechanisms of beta-lactam resistance in anaerobic bacteria. | the known mechanisms of beta-lactam resistance in anaerobic bacteria involve production of beta-lactamases, alteration of penicillin-binding proteins, and blocking of the penetration of beta-lactams through the outer membranes. the most important factor in beta-lactam resistance is the production of beta-lactamase. beta-lactamases in various bacteroides, fusobacterium, and clostridium species have been described. beta-lactam resistance in bacteroides fragilis is most commonly mediated by the pro ... | 1986 | 3541135 |
| isotope exchange at equilibrium studies with rat muscle adenylosuccinate synthetase. | the kinetic mechanism of rat muscle adenylosuccinate synthetase was studied by determining the rates of isotope exchange at equilibrium. a random sequential binding mechanism was indicated for both the forward and reverse reactions. aspartate, adenylosuccinate, gdp, and pi were determined to bind in rapid equilibrium. gtp exchanges with both gdp and pi at the same rate, which is similar to the exchange rate of imp with adenylosuccinate. aspartate exchanges with adenylosuccinate at a higher rate ... | 1986 | 3542024 |
| [experimental therapy in chinese hamsters and rats infected with larval echinococcus multilocularis by using mebendazole, albendazole and ivermectin with brief review of chemotherapy of human multilocular echinococcosis]. | the effects of the mebendazole, albendazole and ivermectin on secondary multilocular echinococcosis in chinese hamsters infected with intraperitoneal inoculation of protoscolices and in rats infected with transportal inoculation of protoscolices were investigated. a reduction in weight of the hydatids greater than 95% was recorded in chinese hamsters intraperitoneally injected with mebendazole suspension. oral administration of mebendazole moderately inhibited the development of the hydatids. al ... | 1987 | 3546045 |
| single-dose chloroquine therapy for plasmodium falciparum in children in togo, west africa. | chloroquine, in a single dose of 10 mg of base/kg, was given orally to togolese children less than 5 years of age as primary therapy for plasmodium falciparum malaria. a simplified world health organization in vivo method was used, as was a sequential analysis procedure for determining if the drug trial was a success or failure. a total of 178 children in 3 regions were treated; 174 (98%) responded successfully, which required a greater than or equal to 75% reduction in parasites by day 2 and el ... | 1987 | 3555135 |
| evidence suggesting that prp is not the infectious agent in creutzfeldt-jakob disease. | it has been suggested that the infectious agents of scrapie and creutzfeldt-jakob disease (cjd) are 'prions' constituted by a protease resistant glycopeptide, prp. to analyze the role of prp in cjd infectivity we re-evaluated the biochemical characteristics of infectivity. first, when the infectious agent is not aggregated, infectivity is exquisitely sensitive to proteinase k treatment, and therefore a proteinase-k-resistant molecule (e.g. prp) is unlikely to contain information essential for ag ... | 1987 | 3556164 |
| ribosomal protein, histone and calmodulin mrnas are differently regulated at the translational level during oogenesis of xenopus laevis. | the localization of r-protein mrna in subcellular compartments has been analysed. it was observed that the mrna for a representative r-protein (l1) is diffuse in the cytoplasm, as shown by in situ hybridization experiments and that the distribution of rp-mrna between polysomes and light mrnps changes during oogenesis. in early oogenesis this mrna is found mostly in subpolysomal fractions, whereas at the beginning of vitellogenesis (stage ii) it becomes associated with polysomes where it remains ... | 1987 | 3556426 |
| x-ray scattering and diffraction by wet gels of aa amyloid fibrils. | systemic amyloidosis is characterized by the extracellular accumulation of protein fibrils with typical ultrastructural morphology. the persistence in vivo of amyloid fibrils, which is responsible for their serious clinical effects, has been thought to reflect the particular, specific conformation of peptide chains constituting the fibrils. on the basis of earlier structural studies this conformation is generally considered to be almost exclusively anti-parallel beta-sheets. we have re-examined ... | 1986 | 3561252 |
| [action of a weak alternating magnetic field on oxygen transport in the body in nonspecific inflammatory lung diseases]. | | 1987 | 3562070 |
| [action of a weak alternating magnetic field on oxygen transport in the body in nonspecific inflammatory lung diseases]. | | 1987 | 3562070 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced lipid peroxidation in hepatic and extrahepatic tissues of male and female rats. | | 1987 | 3566341 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced lipid peroxidation in hepatic and extrahepatic tissues of male and female rats. | | 1987 | 3566341 |
| effect of hypothermia on intestinal adaptation and carcinogenesis in the rat. | postoperative hyperplasia enhances experimental intestinal carcinogenesis, but the effects of nonsurgical adaptation are uncertain. the tropic and tumour-promoting potentials of moderate hypothermia were tested in two groups of male sprague-dawley rats housed at 10 degrees c for 30 weeks. one group (n = 10) received a 6-week course of azoxymethane (total dose 90 mg kg-1). the second group (n = 7) acted as hypothermic controls. another 2 groups maintained at 22 degrees c received azoxymethane (n ... | 1987 | 3567060 |
| abnormal calcium handling by platelets in thrombotic disorders. | this study presents a quantitative comparison of the free cytoplasmic calcium concentration ([ca2+]cyt) and the free concentration in the lumen of the dense tubules of the human platelet. the former was measured by the fluorescence of the high affinity indicator quin2 and latter by the fluorescence of chlorotetracycline (ctc). the ctc technique monitors calcium-ctc complex accumulation in the lumen of dense tubules and mitochondria when washed platelets were incubated in 2 mm ca2+. resting cytop ... | 1987 | 3581444 |
| bacteria-phagocyte interactions: fusobacterium-induced secretion of a neutrophil self-regulatory factor. | direct interaction between fusobacterium nucleatum and human neutrophils resulted in the secretion of a neutrophil self-regulatory factor(s). the secretion of this factor was bacteria specific, and depended on the integrity of the bacteria cell surface. factor secretion occurred within 15 min of bacteria--neutrophil interaction. pre-treatment of neutrophils with cytochalasin b but not sodium fluoride inhibited factor secretion. the factor was sensitive to trypsin and heat treatment. ultrafiltrat ... | 1987 | 3583320 |
| direct modulation of human neutrophil adherence by coaggregating periodontopathic bacteria. | direct interaction between fusobacterium nucleatum and pmns produced consistent enhancement of pmn adherence. in contrast, consistent suppression of pmn adherence was observed after direct interactions between pmns and bacteroides gingivalis or actinomyces viscosus. the modulatory effects of these periodontopathic bacteria on pmn function could be seen as early as 2 min after interaction. the effects were abrogated by alterations of bacterial structural integrity with heat, formalin or ultrasoni ... | 1987 | 3583410 |
| [proof of illegitimacy and identifying the true father after 70 years]. | a man born during the first world war doubted his legitimacy and believed his true father to be his mother's (since deceased) cohabitant. his and his sister's findings in less than or equal to 24 blood group systems produced a probability of sibship of w = 33%. the probability of half-sibship between the proband and two of the cohabitant's legitimate children was w = 82%, between him and two other children w = 70%, and between him and yet another two children w = 73%. these values raised doubts ... | 1987 | 3591019 |
| renal handling of thyroxine, 3,5,3'- and 3,3',5'-triiodothyronine, 3,3'- and 3',5'-diiodothyronine in man. | the 24-h urinary excretion and renal clearance of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), 3,3'-diiodothyronine (3,3'-t2), and 3',5'-diiodothyronine (3',5'-t2) were measured in 17 healthy subjects. the median urinary excretion was (pmol/24h) t4: 1242, t3: 828, rt3: 12.9, 3,3'-t2: 331, and 3',5'-t2: 5.8. the corresponding renal clearances were in median (ml/min) t4: 31, t3: 133, rt3: 15, 3,3'-t2: 683, and 3',5'-t2: 4.5. the clearances differed mutually (p less ... | 1987 | 3591221 |
| renal handling of thyroxine, 3,5,3'- and 3,3',5'-triiodothyronine, 3,3'- and 3',5'-diiodothyronine in man. | the 24-h urinary excretion and renal clearance of thyroxine (t4), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (rt3), 3,3'-diiodothyronine (3,3'-t2), and 3',5'-diiodothyronine (3',5'-t2) were measured in 17 healthy subjects. the median urinary excretion was (pmol/24h) t4: 1242, t3: 828, rt3: 12.9, 3,3'-t2: 331, and 3',5'-t2: 5.8. the corresponding renal clearances were in median (ml/min) t4: 31, t3: 133, rt3: 15, 3,3'-t2: 683, and 3',5'-t2: 4.5. the clearances differed mutually (p less ... | 1987 | 3591221 |
| the effects of stimulus area and adaptation temperature on warm and heat pain thresholds in man. | the effect of stimulus area and adaptation temperature on warm and heat pain threshold were studied in healthy human subjects using a contact thermal stimulator. the stimulus area was varied in the range of 1.3 to 11.8 cm. both the warm and heat pain thresholds decreased with increasing stimulus surface. heat pain and warm thresholds were equally affected by the stimulus surface. the adapting temperature was varied in the range 25-35 degrees c, and warm thresholds were elevated with increasing a ... | 1987 | 3596930 |
| [studies on dynamic posture in normal persons and scoliotic patients]. | dynamic posture of 127 normal males, 144 normal females and 68 scoliotic patients was studied by a system utilizing moiré topography, a video recorder and apple ii microcomputer system. the normal group was divided into five subgroups by age and the scoliotic group into three subgroups according to the cobb angle. the following results were obtained: the clockwise rotation during on-the-spot marching was larger in early juvenile females. the rotation movement during on-the-spot marching was almo ... | 1987 | 3598288 |
| does syphilis facilitate sexual acquisition of hiv? | | 1987 | 3599340 |
| [reconsideration of the 'bacteriological examination' of slaughter stock]. | a comparative trial was made by six meat inspection services to attain uniform indications for the 'bacteriological examination' of slaughtered animals in accordance with the 'meat inspection regulations' of the dutch 'meat inspection act'. the results of the study compelled the investigators to scrutinize these regulations. the 'bacteriological examination' which is prescribed, is designed to exclude septicaemic animals from human consumption. applying the rules will result in condemnation of a ... | 1987 | 3603543 |
| tunica media changes in the spontaneously hypertensive rat (shr). | the histological, ultrastructural, morphometrical and histochemical aspects of the arterial media were studied in young and aged shr, and compared to normotensive wistar kyoto rats. the diffuse thickening was the most characteristic feature of the hypertensive media. it seems due to three processes: early generalized hypertrophy of smooth muscle cells (smc); connective matrix neogenesis and smc proliferation, more evident in peripheral vasculature. the present paper discusses the following hyper ... | 1987 | 3606728 |
| node of ranvier formation along fibres regenerating through silicone tube implants: a freeze-fracture and thin-section electron microscopic study. | thin-section and freeze-fracture electron microscopy have been used to examine the morphogenesis of the node of ranvier in peripheral nerves regenerating through silicone tubes. a major question posed by this study is whether node formation in fibres regenerating across a gap recapitulates that occurring in normal development. node formation occurs concurrently with myelination and follows a similar spatial gradient of progression from a proximal to distal direction along the regenerated nerve. ... | 1987 | 3612184 |
| effects of intraneural injection of ricinus communis agglutinin-60 into rat vagus nerve. | the dorsal motor nucleus (dmn) of the rat was studied at various survival periods following an intraneural injection of ricinus communis agglutinin-60 (rca-60) into the vagus nerve at the mid-cervical region. no obvious structural changes were noted in the dmn 2 and 4 days after the injection of rca-60. at 5 and 6 days after the rca-60 injection, the larger neurons (measuring 19 x 12 microns) in the dmn underwent chromatolytic degeneration whereas the smaller ones (measuring 10 x 6 microns), cha ... | 1987 | 3612185 |
| [use of polypeptides from the vascular wall in the therapy of acute pancreatitis in rats in an experiment]. | | 1987 | 3615005 |
| marginal mandibulectomy for malignant disease: indications, rationale, and results. | the theoretical basis for performing marginal mandibular resection, as opposed to segmental resection, is discussed; and criteria are suggested. results are presented that suggest that, in the treatment of malignant disease around the jaw, although the mortality from other tobacco- and alcohol-related diseases remains high, good functional and cosmetic results can be achieved with no loss of local control. | 1987 | 3625019 |
| bioequivalence and pharmacokinetic profile of promethazine hydrochloride suppositories in humans. | a bioequivalence study of promethazine hydrochloride (10-[2-(dimethylamino)propyl]-phenothiazine monohydrochloride) was conducted in 20 male human subjects with the purpose of comparing, under blind condition, the human serum levels of promethazine in three different formulations. the formulations tested were a 50-mg promethazine hydrochloride polyethylene glycol suppository, a 50-mg promethazine hydrochloride cocoa butter-white wax suppository, and a 50-mg oral dose of promethazine hydrochlorid ... | 1987 | 3625487 |
| bioequivalence and pharmacokinetic profile of promethazine hydrochloride suppositories in humans. | a bioequivalence study of promethazine hydrochloride (10-[2-(dimethylamino)propyl]-phenothiazine monohydrochloride) was conducted in 20 male human subjects with the purpose of comparing, under blind condition, the human serum levels of promethazine in three different formulations. the formulations tested were a 50-mg promethazine hydrochloride polyethylene glycol suppository, a 50-mg promethazine hydrochloride cocoa butter-white wax suppository, and a 50-mg oral dose of promethazine hydrochlorid ... | 1987 | 3625487 |
| a computerized system for measuring detection sensitivity over the visual field. | a device is described for comprehensively measuring the capability of human operators to detect and react to visual signals occurring at a variety of locations throughout the functional visual field. the system is completely automated and computerized, and provides both documentation files and descriptive graphics of the operator's performance immediately upon completion of testing. sensitivity of detection can be measured for red, yellow, and green stimulus colors in a testing configuration whi ... | 1987 | 3632544 |
| kaposi's sarcoma: a trifactorial model. | to explain the epidemiology of kaposi's sarcoma, including its predominance in men, i present a theory based on three concurrent factors: immunodeficiency, exposure to a specific viral agent, and a recessive, highly prevalent x-linked regulatory gene. the x-linked locus is inferred from comparing the aberrant lymphaticovenous nature of immature lesions with absence of lymphaticovenous connections in xo karyotypes. the theory predicts that for heterosexual populations without sex differences in v ... | 1987 | 3647226 |
| multiple lineage colony growth from human marrow in plasma clot diffusion chambers. | marrow cells from ten healthy adult donors were cultured in plasma clot diffusion chambers implanted intraperitoneally into mice. host animals were conditioned by two injections of phenylhydrazine and 600 cgy of x-rays. cultures (5 x 10(4) cells/chamber) were continued for between 2 and 40 days and the chambers were retransplanted into new host animals every 5 days. following termination of cultures, plasma clots were stained with benzidine-hematoxylin and analyzed microscopically. erythroid, ne ... | 1987 | 3653298 |
| reacquisition of classical conditioning after removal of cerebellar cortex. | we trained rabbits with white noise and light conditioned stimuli and a face shock unconditioned stimulus for classical conditioning of the nictitating membrane response and then removed the ipsilateral cerebellar cortex by aspiration. larsell's hemisphere lobule vi was completely removed in 5 rabbits; the pattern of degeneration in the inferior olive matches the projections to hvi reported in experiments with horseradish peroxidase. all rabbits showed an initial abolition of conditioned respons ... | 1987 | 3653317 |
| the effect of corneal contact lenses on the oxygen tension in the anterior chamber of the rabbit eye. | the oxygen tension in the aqueous humor in the anterior chamber of rabbit eyes was measured continuously with a polarographic electrode. the normal oxygen tension in the anterior chamber was 23 +/- 2 mm hg (mean +/- sd, n = 4). a contact lens was then placed on the cornea for at least 10 min and the drop in oxygen tension recorded. a hard polymethylmethacrylate lens reduced the oxygen tension by 16 +/- 4 mm hg, and a larger hydroxyethylmethacrylate soft lens (soflens) decreased oxygen tension by ... | 1987 | 3654146 |
| synthesis and assembly of membrane skeletal proteins in mammalian red cell precursors. | the synthesis of membrane skeletal proteins in avian nucleated red cells has been the subject of extensive investigation, whereas little is known about skeletal protein synthesis in bone marrow erythroblasts and peripheral blood reticulocytes in mammals. to address this question, we have isolated nucleated red cell precursors and reticulocytes from spleens and from the peripheral blood, respectively, of rats with phenylhydrazine-induced hemolytic anemia and pulse-labeled them with [35s]methionin ... | 1987 | 3654760 |
| chemical composition and immunobiological activities of sodium dodecyl sulphate extracts from the cell envelopes of actinobacillus actinomycetemcomitans, bacteroides gingivalis and fusobacterium nucleatum. | the chemical composition and immunobiological activities in vivo and in vitro of sodium dodecyl sulphate extracts (sds-se) derived from periodontopathic bacteria (three strains of actinobacillus actinomycetemcomitans, two strains of bacteroides gingivalis, and one strain of fusobacterium nucleatum) were investigated. the main components of sds-se were protein and lipid, with negligible amounts of peptidoglycan and lipopolysaccharide. immunopotentiating activity was detected in both delayed-type ... | 1987 | 3655733 |
| attachment of oral bacteria to a basement-membrane-like matrix and to purified matrix proteins. | the purpose of this study was to investigate the adherence of oral bacteria to an in vitro basement-membrane-like matrix and to selected individual macromolecular constituents of this matrix. radiolabeled bacteria were incubated with basement-membrane-like matrices isolated from pf hr-9 cells. bacteroides gingivalis 33277, fusobacterium nucleatum fn-2, and actinobacillus actinomycetemcomitans ga3(a) bound to the matrix in the range of 44 to 70%, considerably higher than the ranges of a. actinomy ... | 1987 | 3666961 |
| [housing and health of farm animals]. | data on the relationship between housing systems and health in farm animals are relatively scarce. in dairy cattle and poultry, for which special housing systems are dominant in the netherlands, no important new figures are available. in veal calves, the group housing system is being investigated. preliminary results indicate that there are many problems in the first 6 to 8 weeks. more research has been done on pigs. it is now clear that the floor system is important not only with respect to leg ... | 1987 | 3672468 |
| enrichment of subgingival microflora on human serum leading to accumulation of bacteroides species, peptostreptococci and fusobacteria. | this study was undertaken to identify ecological factors that favour opportunistic pathogenic species in the subgingival microflora. in a first approach, human serum as a substitute for gingival exudate, was used for batch-wise enrichment of subgingival plaque. the microflora resulting after 5-6 enrichment steps consisted of black-pigmented and non-black-pigmented bacteroides species, peptostreptococcus micros and fusobacterium nucleatum as the main organisms. it is noted that the same group of ... | 1987 | 3674857 |
| hemagglutinating activity of lipopolysaccharides from subgingival plaque bacteria. | lipopolysaccharide (lps) preparations from the bacteroides species b. endodontalis, b. intermedius, b. denticola, and b. melaninogenicus and from fusobacterium nucleatum, haemophilus actinomycetemcomitans, capnocytophaga gingivalis, and eikenella corrodens directly agglutinated erythrocytes of some kinds of animals. lpss from the bacteroides species b. gingivalis, b. asaccharolyticus, b. corporis, and b. loescheii and from capnocytophaga ochracea did not possess any hemagglutinating activity. pr ... | 1987 | 3679548 |
| practolol inhibits human skin fibroblast cell mat hydroxyproline accumulation. | despite being poorly absorbed practolol (n-4-2-hydroxy-3-(1-methyl-ethyl)-amino propoxy phenyl acetamine) inhibited the accumulation of cell mat hydroxyproline, a measure of collagen synthesis, by human skin fibroblasts (dt2ph) in vitro, (id50 0.8 x 10(-3) m). the degree of inhibition was dependent on the concentration of practolol used and the incubation time. neither preinitiation of collagen synthesis nor omitting ascorbic acid from the incubation medium modified this inhibitory action. in co ... | 1987 | 3687592 |
| dna probes for identification of clinically important bacteroides species. | conventional procedures for identifying gram-negative anaerobes such as bacteroides spp. are cumbersome and time-consuming. a simpler approach would be to use dna probes to identify these organisms. since many different species of gram-negative anaerobes are isolated from clinical specimens, the most useful dna probes would be probes that identify a few clinically significant groups of anaerobes. to obtain such probes, we cloned hindiii-digested chromosomal dna from various bacteroides species a ... | 1986 | 3700617 |
| [lithotripsy by ultrashort pulsation. experimental study in renal lithiasis in the dog]. | a lithotriptor delivering ultrashort shockwaves and designed for use under ultrasonographic guidance has recently been developed. immersion in water is unnecessary. the device was experimented in renal lithiasis in dogs. twenty-two animals were treated. in seven, who served as controls, the safety of shots delivered in particular to the lungs, liver, bones and kidneys was demonstrated. furthermore, no cardiac rhythm disorders were recorded. a human stone was implanted in 15 animals. the stone wa ... | 1986 | 3707073 |
| local tolerance evaluation of intramuscular drug preparations. | | 1986 | 3709769 |
| sensitization with fusobacterium nucleatum targets antibody-dependent cellular cytotoxicity to mammalian cells. | incubation of mammalian tumor cells with either soluble of insoluble fractions (10 to 100 micrograms/ml) of fusobacterium nucleatum sensitizes them to the destructive activity of antibody-dependent cellular cytotoxicity (adcc) effector cells in the presence of anti-f. nucleatum antisera. all three types of adcc effector cells are capable of destroying f. nucleatum-sensitized target cells with varying degrees of effectiveness (lymphocytes much greater than monocytes greater than neutrophils). hyp ... | 1986 | 3710577 |
| bvd virus infection and embryo transfer. | | 1986 | 3716157 |
| absence of role of endothelium in the response of isolated porcine coronary arteries to acetylcholine. | isolated precontracted arteries of various vascular beds relax in response to acetylcholine only if the endothelium is present. one explanation for this is that this drug stimulates the endothelial cells to release a vasodilator substance that in turn relaxes the underlying smooth muscle. to determine whether this mechanism is concerned also in the acetylcholine contraction of isolated porcine coronary arteries transverse strips of the extramural part of the left circumflex artery were used for ... | 1986 | 3719611 |
| dietary protein, enhancement of n-nitrosomethylurea-induced mammary carcinogenesis, and their effect on hormone regulation in rats. | the effect of supplemental dietary protein (casein) fed with high fat diets was investigated using the n-nitrosomethylurea-induced mammary tumor model. isocaloric diets containing casein and corn oil at 19 and 15% (normal protein-high fat) or 33 and 15% (high protein-high fat) were fed ad libitum to sprague-dawley mother rats. female offspring continued on the diet. food consumption and growth curves were similar over the entire growth period. n-nitrosomethylurea (50 mg/kg body weight) or saline ... | 1986 | 3731097 |
| 3,3',5'-triiodothyronine inhibits iodothyronine-5'-deiodinating activity induced by 3,5,3'-triiodothyronine at equimolar concentrations in cultured fetal mouse liver. | to investigate the rt3 effect on iodothyronine-5'-deiodinating activity (i-5'-da) in cultured fetal mouse liver, liver on the 19th day of gestation, in which little or no i-5'-da was detected and, therefore, rt3 was very stable, was cultured in medium containing thyroid hormone-depleted fetal calf serum supplemented with cortisol, insulin, and various concentrations of iodothyronines. after 4-15 days of culture, i-5'-da in the homogenate was assessed by the amount of iodide released from outer r ... | 1986 | 3732155 |
| demonstration of 20k growth hormone in human plasma by gel electrophoretic-immunostaining-autoradiographic assay (geisaa). | a variant of human growth hormone (hgh), in which 15 amino acids are missing (commonly referred to as 20k-hgh in contrast to the traditional form which is referred to as 22k-hgh), is known to exist in human pituitary glands. however, lack of a method to measure it in blood has hindered investigations of its physiopathology. we have applied a newly-developed technique called geisaa for its detection in small volumes of human plasma. the method is based upon the lower mr of the variant and its abi ... | 1986 | 3732988 |
| the ionization behavior of bile acids in different aqueous environments. | the ionization behavior of cholic acid, deoxycholic acid, and chenodeoxycholic acid in a variety of physiologically important molecular environments was studied using 13c nmr spectroscopy. the apparent pka of the carboxyl group was determined from titration curves obtained from the dependence of the carboxyl carbon chemical shift on ph. using 90% 13c isotopic substitution of the carboxyl carbon, a complete titration curve was obtained for cholate at a concentration below its critical micelle con ... | 1986 | 3734630 |
| nitrogen conversion factors for the proteinaceous content of gums permitted as food additives. | nitrogen conversion factors for gum arabic (acacia senegal (l.) willd.), gum tragacanth (asiatic astragalus spp.), gum karaya (sterculia spp.), guar gum (cyamopsis spp.), locust bean (carob) gum (ceratonia spp.), tara gum (caesalpinia spp.), and xanthan gum (xanthomonas campestris) have been calculated from data for the amino acid compositions of their proteinaceous components. the factors derived differ from the arbitrary values (5.7 or 6.25) at present specified by international regulatory aut ... | 1986 | 3743832 |
| concentration-dependence of halothane metabolism in rabbits. | | 1986 | 3744878 |
| biochemistry of maternal milk in early lactation. | the analysis of more than 550 human milk samples showed that triglycerides (accounting for 96-99 per cent of the total lipids) increased from 2 to 3.5 g/100 ml mainly during the first week postpartum and remained constant thereafter. in contrast, both cholesterol and vitamin e concentrations decreased from 35 to 20 mg/100 ml and from 1.7 to 0.30 mg/100 ml, respectively. the phospholipids remained constant (40 mg/100 ml). the fatty acid composition of total lipids showed remarkable changes. mid-c ... | 1986 | 3744886 |
| diplostomum spathaceum (rud. 1819): effects of physical factors on the infection of rainbow trout (salmo gairdneri) by cercariae. | the relationship between infection rates of rainbow trout (salmo gairdneri) by diplostomum spathaceum and cercarial concentration, water flow rate and temperature were investigated by means of controlled infections within a flume. a linear relationship was obtained between cercarial concentration and mean abundance of metacercariae/fish. a biphasic relationship occurred between flow rate and abundance of metacercariae. within the confines of the flume, it was possible to control the infection ra ... | 1986 | 3748615 |
| [procedure for the biological testing of substances in the guinea pig subcutaneous test. i. the results of an improved semiquantitative assessment of the histological criteria]. | different materials were implanted into the subcutaneous connective tissue of guinea pigs to evaluate biotolerance by means of the tissue response. the investigations were carried out with reference materials and newly developed implant materials in short-term studies and in case control studies. the semiquantitative valuation standards lead mostly only to informing statements in the sense of a compatibility degree. alternative assertions are possible when comparing very different materials. | 1986 | 3751202 |
| spinal and supraspinal mechanisms for morphine-pentobarbital antinociceptive interaction in relation to cardiac acceleration response in rats. | in rat experiments, morphine-pentobarbital antinociceptive interaction affecting cardiac acceleration in response to somatic noxious stimulation was analyzed with the use of intrathecal and intracerebroventricular injections of morphine. cardiac acceleration response was induced by tail compression, and heart rate was monitored by electrocardiogram. pentobarbital, in a subanesthetic intravenous dose, antagonized the antinociceptive effect of morphine in relation to cardiac acceleration response ... | 1986 | 3754702 |
| epidemiologic studies on possible health effects of intake of pyrolyzates of foods, with reference to mortality among japanese seventh-day adventists. | to elucidate the effect of intake of mutagenic and/or carcinogenic pyrolysis products of proteins and amino acids on carcinogenesis in man, we have undertaken two epidemiologic cohort studies: one concerning the possible association of broiled fish consumption with cancer and the other concerning the cancer mortality among japanese seventh-day adventists. the main findings of these studies are described. | 1986 | 3757950 |
| [effects of ambroxol on pulmonary surfactant--analysis of the fatty acid composition of phosphatidylcholine in the sputum and normal respiratory tract fluid in rabbits]. | the mechanism of action of secretagogic expectorants (ex., ambroxol) has not been clarified. recently, attention has been directed to the relationship of their action to pulmonary surfactants. in the present study, the fatty acid (fa) composition of phosphatidylcholine (pc), which is the main physiological surfactant, was investigated using sputum, respiratory tract fluid, mucin-like substance, lung washings and lung tissue of rabbits. effects of ambroxol (20 mg/kg, i.s.) on several parameters s ... | 1986 | 3758876 |
| progesterone receptor synthesis and degradation in mcf-7 human breast cancer cells as studied by dense amino acid incorporation. evidence for a non-hormone binding receptor precursor. | we have used the technique of density labeling of proteins by biosynthetic incorporation of 2h, 13c, 15n (dense) amino acids to study the synthesis and degradation rates of the progesterone receptor in mcf-7 human breast cancer cells. in cells grown in the absence of progestin, sucrose gradient shift analyses reveal that it takes 17 h for the normal density progesterone receptor levels to be reduced to half the initial value, whereas in the presence of 10 nm of the synthetic progestin [3h]r5020, ... | 1986 | 3759961 |
| outer membrane proteins of fusobacterium nucleatum fev1. | outer membrane enriched material from six strains of fusobacterium nucleatum was analysed by sds-page. the protein profiles of all the strains were dominated by proteins with molecular masses of about 40 kda, and a very high degree of homology in relation to apparent molecular masses was observed. in all strains except fev1, one of the most dominant proteins exhibited heat modifiable properties, having an apparent molecular mass of about 38 kda and 42 kda when heated in sds at 50 and 100 degrees ... | 1986 | 3760817 |
| clinical, pathogenetic, and laboratory features of capnocytophaga infections. | granulocytopenia and oral mucosal defects have been reported to be important predisposing factors to recently recognized cases of capnocytophaga septicemia. the authors call attention to an apparent preponderance of these cases in the pediatric age group and emphasize laboratory features which they have found helpful in the diagnosis of capnocytophaga infections. thirteen patients with capnocytophaga infections were seen during a seven-year period. seven of these patients had capnocytophaga bact ... | 1986 | 3766465 |
| action of surfactants on porcine heart malate dehydrogenase isoenzymes and a simple method for the differential assay of these isoenzymes. | the cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (ctab), completely inactivates porcine heart cytoplasmic malate dehydrogenase (l-malate:nad+ oxidoreductase, ec 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. these concentrations are close to, or higher than, the critical micelle concentration of ctab. an increase in the ionic strength of the medium significantly retards the ctab-induced inactivation of the cytoplas ... | 1986 | 3768404 |
| acute hypokalemic effect of gossypol. | clinical trials had demonstrated that chronic ingestion of gossypol induced infertility in males. hypokalemia of various severities were reported in volunteers taking gossypol. the purpose of this study is to investigate the effects of acute gossypol infusion into alkalotic rats. plasma potassium concentration decreased from 4.08 to 2.87 mm after gossypol infusion with minimal change in urinary potassium excretion. the hypokalemic effect of gossypol was also observed in nephretomized rats. some ... | 1986 | 3768796 |
| acute yersinia infections in man increase intestinal permeability for low-molecular weight polyethylene glycols (peg 400). | the passive intestinal permeability of patients with acute diarrhoea was investigated with a liquid meal of oral rehydration fluid containing differently sized, low-molecular weight polyethylene glycols (peg 400). the recovery of test molecules was measured in 6 h specimens of urine. the subjects suffered from acute infections caused by yersinia bacteria accompanied by joint pains. they were investigated during the infection and at least 3-4 weeks later when they had recovered clinically. it was ... | 1986 | 3775268 |
| acute yersinia infections in man increase intestinal permeability for low-molecular weight polyethylene glycols (peg 400). | the passive intestinal permeability of patients with acute diarrhoea was investigated with a liquid meal of oral rehydration fluid containing differently sized, low-molecular weight polyethylene glycols (peg 400). the recovery of test molecules was measured in 6 h specimens of urine. the subjects suffered from acute infections caused by yersinia bacteria accompanied by joint pains. they were investigated during the infection and at least 3-4 weeks later when they had recovered clinically. it was ... | 1986 | 3775268 |
| [changes in the structure of pulmonary blood vessels and bronchi in progressive bronchial stenosis]. | | 1986 | 3781307 |