| assessment of exposure to sexually transmitted agents other than human papillomavirus. | there are now a number of lines of evidence giving support for a causal role of high-risk oncogenic hpv types in cervical cancer. however, it is clear that only a minority of women with hpv 16 infection, for example, develop invasive cancer. therefore examination of whether sexually transmitted agents other than hpv are interacting in the genesis of cervical neoplasia is necessary. serological methods which accurately reflect past exposure to individual agents are ideal for epidemiological studi ... | 1992 | 1330921 |
| the use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. | ocular swabs from 30 consecutive patients with follicular conjunctivitis were screened for chlamydia trachomatis, herpes simplex virus, and adenovirus by a variety of laboratory techniques. for the detection of c. trachomatis, we compared two polymerase chain reaction methods, mccoy cell culture isolation, and the direct fluorescein-conjugated monoclonal antibody test. four of 30 patients tested positive for c. trachomatis by using both conventional and biotinylated polymerase chain reaction met ... | 1992 | 1334375 |
| cytologic evidence of the association of different infective lesions with dysplastic changes in the uterine cervix. | cervical smears from 4055 women were examined and classified as per who criteria. 873 (21.53%) smears revealed dysplastic changes. the dysplastic smears were further examined cytologically for the presence of chlamydia trachomatis, herpes simplex virus type-2, human papilloma virus, trichomonas vaginalis and candida albicans. chlamydia was found to be the commonest micro-organism associated with cervical dysplasia followed by herpes simplex virus type-2 and human papilloma virus. the association ... | 1992 | 1336729 |
| azithromycin in lower respiratory tract infections. | azithromycin is a new azalide antimicrobial agent which has a broad spectrum of activity against common lower respiratory tract pathogens including pneumococci, staphylococci, legionella species, mycoplasma and chlamydia species. in particular, it is more active against haemophilus influenzae than other macrolides. in comparison to other new macrolides, azithromycin achieves higher tissue and intracellular concentrations and these concentrations are sustained for several days after dosing due to ... | 1992 | 1336893 |
| azithromycin (sumamed-pliva) in the treatment of chlamydial genital tract infections in infertile marriages. | | 1992 | 1337473 |
| in vitro activity of sparfloxacin and other antimicrobial agents against genital pathogens. | the in vitro activity of sparfloxacin was determined for 60 strains of neisseria gonorrhoeae, 15 strains of chlamydia trachomatis and 40 strains each of gardnerella vaginalis, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ampicillin, azithromycin, clarithromycin, erythromycin, ofloxacin, temafloxacin and tetracycline. sparfloxacin was active against all the strains studied and appeared to be the most potent quinolone tested. sparfloxacin had the lowest mics against n. ... | 1992 | 1337506 |
| [an outbreak of ovine granular keratoconjunctivitis of chlamydial origin, in ivory coast]. | during the dry season 1990-1991, an outbreak of ovine granular keratoconjunctivitis occurred in côte-d'ivoire. the chlamydial etiology was demonstrated. all flocks were affected, with a morbidity rate of 30 to 70%. lesions of keratitis were observed in 5 to 15% of the sick animals. the treatment with auréomycine (nd-specia, ophthalmic ointment) was constantly efficient. | 1992 | 1338737 |
| similarity of chlamydia pneumoniae strains in the variable domain iv region of the major outer membrane protein gene. | dna was amplified by polymerase chain reaction from the gene encoding the major outer membrane protein (momp) of chlamydia pneumoniae in order to examine the relatedness of strains isolated from diverse geographical regions. primers for this reaction were chosen to span a 207-bp region comparable to that of the fourth variable segment of the momp gene of chlamydia trachomatis. among c. trachomatis, sequence heterogeneity is characteristic within variable sequence domain iv (vdiv) and correlates ... | 1992 | 1339411 |
| [biological products and laboratory reagents dehydrated by a rapid direct vacuum desiccation method. vi. studies of the preservation of microbial strains (c. burnetii, chlamydia psittaci, mycoplasma pneumoniae) and a laboratory reagent (para-nitrophenyl phosphate) by vacuum desiccation]. | | 1992 | 1340260 |
| [the prevalence of chlamydia trachomatis infections in gynecologic diseases]. | | 1992 | 1340263 |
| [urogenital infections produced by chlamydia trachomatis]. | | 1992 | 1340265 |
| the fitz-hugh-curtis syndrome in a man revealed by ectopic appendicitis. | | 1992 | 1341616 |
| molecular biology of chlamydiae. | | 1992 | 1341644 |
| recurrent tonsillitis. the role of chlamydia and mycoplasma. | the core tissue microflora of 40 patients who underwent tonsillectomies were examined with cultures, dna probe tests, enzyme immunoassays, and direct immunofluorescence antibody tests for chlamydia and mycoplasma. we believe this is the largest and most accurate prospective study that has examined the role of chlamydia and mycoplasma in the core tonsil tissue of patients with recurrent or chronic tonsillitis. the data strongly indicate that, unlike acute tonsillopharyngitis, chlamydia and mycopl ... | 1992 | 1341878 |
| [clinical laboratory studies in acute chlamydial, gonococcal and nonchlamydial nongonococcal salpingitis]. | the authors carried out a study on 135 patients, hospitalized for acute salpingitis, based on data of history, clinical and laboratory examination. the latter were analysed regarding the isolated etiological agent. | 1992 | 1342144 |
| [cervical and abdominal microbiology in infertile patients]. | the microbiology of twenty-nine asymptomatic infertile women was evaluated prospectively. samples were taken from the cervix and the endosalpinx during laparoscopy. cultures for aerobes, anaerobes, neisseria gonorrhoeae, mycoplasma, ureaplasma urealyticum and chlamydia trachomatis were performed. no association was found between the presence of germs in the abdomen or cervix and tubal abnormalities. history of previous iud use was associated with tubal disease. | 1992 | 1342453 |
| [our experience in the diagnosis of chlamydial cervicitis with the chlamydiasyme and chlamyset antigen tests]. | the authors analyse in the present study the results of the examination of 309 female patients for chlamydial infection, using chlamyset antigen (orion diagnostica--finland) and chlamydiasyme diagnostic kit (abbott labs--usa). chlamydial antigen was found in the endocervix of 15.2% of the examined cases. comparing the enzyme immunomethod chlamydiasyme with the immunofluorescent method chlamyset antigen the authors found 94.4% diagnostic accuracy. | 1992 | 1342550 |
| nonculture tests for genital tract chlamydial infection. what does the package insert mean, and will it mean the same thing tomorrow? | | 1992 | 1344066 |
| challenge of chlamydia research. | chlamydia is an obligate intracellular bacterial pathogen that severely challenges the patience and creativity of all its investigators--even to the point that some investigators have forsaken this field for more productive and fertile areas of research. the two principal difficulties that touch every aspect of chlamydial research are (a) that chlamydiae only grow within eukaryotic host cells and (b) there are limited genetic approaches available. despite these technical difficulties, the fundam ... | 1992 | 1344667 |
| [diagnosis of ocular chlamydiosis by gene amplification (polymerase chain reaction or p.c.r.)]. | identification of conjunctival chlamydioses could be very difficult since this disease is extremely latent. moreover previous instillation of topical antibiotic provokes a negativation of d.i.f. and of cultures. p.c.r. is very useful, it was demonstrative in 41 cases out of 47 cases; d.i.f. was positive only in 18 cases. p.c.r. is a very promising method to recognize chlamydiae; identification of different serovars is processing. | 1992 | 1344966 |
| berberine, a potential drug for trachoma. | the clinical serological response to topical treatment of trachoma with berberine an indigenous drug has been studied in 32 microbiologically confirmed cases. efficacy of berberine 0.2% when compared to sulfacetamide 20% was found to be superior in both the clinical course of trachoma and in achieving a fall in the serum antibody titers (p < 0.05) against chlamydia trachomatis in the treated patients. | 1992 | 1344968 |
| [health benefits of contraception]. | the benefits and side effects of various contraceptive methods are summarized. the condom is supposed to protect from hiv transmission. some studies claim that the condom protects from hiv at an effectiveness rate of 60%. in combination with a spermicide such as nonoxynol-9, it offers especially good protection against gonococci and chlamydia, according to investigations carried out in bangkok, thailand. similarly, chlorhexidine protects from hiv infections. a chinese study claimed that the cont ... | 1992 | 1345289 |
| [sensitivity and specificity of mouse peritoneal macrophages in the diagnosis of chlamydia trachomatis infections]. | we analyzed the sensitivity and specificity of mouse peritoneal macrophages (mpm) for the diagnosis of infection by chlamydia trachomatis. fifty seven samples were studied, 25 of them were from cervix and 32 were from bronchial aspiration, resulting 29 positive by immunofluorescence in mccoy cells and only 16 in mpm. the sensitivity and specificity for mpm were 55% and 96% respectively. we concluded that mccoy cells are the election cells for the diagnosis of chlamydia infection. however, the mp ... | 1992 | 1345308 |
| chlamydial infections: therapeutic options. | | 1992 | 1347588 |
| does infection occur with modern intrauterine devices? | it is difficult to determine if the iud increases the risk of pelvic inflammatory disease (pid) because simple clinical features are not consistently predictive and can have low specificity and sensitivity. the c-reactive protein and the erythrocyte sedimentation rate tests help with pid diagnosis, but only a laparoscopy can determine tubal involvement. in 1970, who's cooperative statistical programme found 2-year combined pid rates to range from 3.8 to 5.2/100 women with an iud. then who an ... | 1992 | 1347810 |
| urease-associated heat shock protein of helicobacter pylori. | helicobacter pylori urease is an extracellular, cell-bound enzyme with a molecular weight of approximately 600,000 (600k enzyme) comprising six 66k and six 31k subunits. a 62k protein is closely associated with the h. pylori urease, both in crude preparations and after gel filtration; this protein can be removed from the urease by ion-exchange chromatography without inactivating the enzyme. we purified this urease-associated protein and determined its n-terminal amino acid sequence. the sequence ... | 1992 | 1348725 |
| chlamydial and rickettsial transmission through tick bite in children. | | 1992 | 1348825 |
| amoxycillin/clavulanic acid (augmentin) compared with triple drug therapy for pelvic inflammatory disease. | sixty jordanian women with pelvic inflammatory disease (pid) were studied. of these, 31 were given oral amoxycillin/clavulanic acid (augmentin) for a mean duration of 8.4 days and 29 were given a standard triple drug regimen of oral ampicillin, intramuscular gentamicin and metronidazole tablets/pessaries for a mean duration of 7.2 days. bacterial culture (cervical and high vaginal swabs) was positive in every case, most often e. coli but sometimes more than one pathogen was isolated. no gonococc ... | 1992 | 1348989 |
| staphylococcus aureus in urine and false-positive immunoassay for chlamydia. | | 1992 | 1349121 |
| chlamydia pneumoniae infection and asthma. | | 1992 | 1349397 |
| chlamydia trachomatis infection in children with wheezing simulating asthma. | | 1992 | 1349398 |
| two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompa dna of chlamydia spp. | specific and sensitive amplification of major outer membrane protein (momp) gene (ompa) dna sequences of chlamydia species with various momp genotypes was achieved by a two-step polymerase chain reaction (pcr). degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial momp genes were used in a pcr to amplify a dna fragment of approximately 1,120 bp. a portion of this dna fragment was amplified in a second genus-specific re ... | 1992 | 1349899 |
| chlamydia and complexes. | | 1992 | 1349943 |
| use of nonoxynol-9 and reduction in rate of gonococcal and chlamydial cervical infections. | the spermicide nonoxynol-9 (n-9) has been used as a contraceptive for over 30 years, but the use of a vaginal spermicide and condoms for the prevention of sexually transmitted infections has not been examined in randomised studies. we report a single-blind randomised field trial to assess the effect of n-9 film on the rate of gonococcal and chlamydial cervical infection in women at high risk of these diseases. 343 women were randomly assigned to use either condoms and n-9 (186 women) or condoms ... | 1992 | 1350804 |
| false-positive immunoassay for chlamydia in urine. | | 1992 | 1350844 |
| vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia. | the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ... | 1992 | 1351005 |
| detection of chlamydia trachomatis dna in joints of reactive arthritis patients by polymerase chain reaction. | in 1986, chlamydia trachomatis elementary bodies were found by direct immunofluorescence (dif) in synovial-fluid cell deposits and synovial-membrane biopsy samples from five of eight patients with sexually acquired reactive arthritis (sara) but in none of eight controls with other types of arthritis. cells from the original slides (stored at 4 degrees c) have now been examined by a polymerase chain reaction (pcr) that amplifies dna for the major outer membrane protein of c trachomatis. chlamydia ... | 1992 | 1352016 |
| the possible role of chlamydia trachomatis in perineal suppurative hidradenitis. | to find out if there is an association between perineal suppurative hidradenitis and chlamydia trachomatis infection. | 1992 | 1352134 |
| chlamydia trachomatis and wheezing. | | 1992 | 1352610 |
| myocarditis caused by chlamydia pneumoniae (twar) and sudden unexpected death in a swedish elite orienteer. | | 1992 | 1353578 |
| [molecular epidemiology of chlamydia trachomatis by dna restriction endonuclease analysis]. | | 1992 | 1357211 |
| prevalence of specific antibodies to chlamydia pneumoniae (twar) in swedish orienteers. | | 1992 | 1357445 |
| biological properties and genetic analysis of the ompa locus in chlamydiae isolated from swine. | eight strains of chlamydia psittaci isolated from swine with pneumonia, pleuritis, pericarditis, and enteritis were characterized through analysis of the major outer membrane protein gene ompa by a two-step polymerase chain reaction, by their interactions with cells in culture, and by the morphologic features and ultrastructure of intracellular inclusions. amplified chlamydial ompa dna fragments were differentiated by restriction endonuclease digestion. chlamydial isolates were separated into 2 ... | 1992 | 1358014 |
| pcr detection and differentiation of chlamydia pneumoniae, chlamydia psittaci and chlamydia trachomatis. | a pcr-based system was developed for the detection and differentiation of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae. a conserved 145 bp fragment of the chlamydial omp1 gene was amplified from all three species. the three species were then differentiated from each other by digestion of this pcr product with restriction enzymes eco ri and either hind iii or pst i. the system was shown to work for two strains of c. pneumoniae, 11 strains of c. psittaci and 10 serovars of c. ... | 1992 | 1361961 |
| distinguishing chlamydia species by restriction analysis of the major outer membrane protein gene. | clinical isolates of chlamydia pneumoniae from diverse geographic locations and strains of other chlamydia species were typed by polymerase chain reaction (pcr) amplification of the major outer membrane protein (momp) gene followed by restriction fragment length polymorphism analysis of the product. use of synthetic primers corresponding to highly conserved regions of the momp gene resulted in amplification of a 1070 bp product in laboratory strains and clinical isolates of c. pneumoniae, c. tra ... | 1992 | 1361962 |
| gene typing of chlamydia trachomatis by polymerase chain reaction and restriction endonuclease digestion. | a portion of the major outer membrane protein (momp) gene from 15 chlamydia trachomatis serovars was amplified by polymerase chain reaction (pcr) and the product was analyzed by restriction fragment length polymorphism (rflp). a set of primers was used to amplify an 871 base pair gene fragment encompassing the 4 hypervariable regions of momp. alui digestion of the product gave distinctive patterns for the 15 serovars as demonstrated on silver-stained polyacrylamide gels. a triple digest with eco ... | 1992 | 1362824 |
| [infections and coronary heart disease]. | | 1991 | 1365349 |
| chlamydial infection in canada. | | 1992 | 1365519 |
| [various therapeutic protocols used in genital disease caused by chlamydia trachomatis]. | | 1991 | 1365577 |
| [action of ++clarithromycin against mycoplasma and chlamydia]. | | 1991 | 1365578 |
| [pelvic inflammatory disease and its treatment]. | | 1992 | 1366085 |
| unknown sequence amplification: application to in vitro genome walking in chlamydia trachomatis l2. | a recently described technique, 'chemical genetics' unknown sequence amplification method, which requires only one specific oligonucleotide, has broadened the applicability of the polymerase chain reaction to dna of unknown sequence. we have adapted this technique to the study of the genome of chlamydia trachomatis, an obligate intracellular bacterium, and describe modifications that significantly improve the utility of this approach. these techniques allow for rapid genomic analysis entirely in ... | 1991 | 1367216 |
| immunogenicity of a chimeric peptide corresponding to t helper and b cell epitopes of the chlamydia trachomatis major outer membrane protein. | the immunogenicity of a chimeric t/b cell peptide corresponding to antigenically characterized epitopes of the chlamydia trachomatis major outer membrane protein (momp) was studied in mice to further define its potential use in the development of a subunit vaccine in preventing blinding trachoma in humans. the chimeric peptide, designated a8-vdi, corresponds to a conserved momp t helper (th) cell epitope(s) (a8, residues 106-130) and serovar a vdi (residues 66-80), which contains the serovar-spe ... | 1992 | 1370528 |
| mycobacteria and human heat shock protein-specific cytotoxic t lymphocytes in rheumatoid synovial inflammation. | to study the cytotoxic capacity of mycobacteria-specific t lymphocyte lines and clones from sites of inflammation in patients with rheumatoid arthritis (ra). we also studied antigen specificity, surface phenotype, expression of t cell receptors (tcr), and hla restriction. | 1992 | 1371388 |
| a synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart. | the tetrasaccharide 3-deoxy-alpha-d-manno-2-octulosonic acid (alpha-kdo) (2----8)-alpha-kdo(2----4)-alpha-kdo(2----6)-beta glcnac, a partial structure of chlamydial lipopolysaccharide (lps) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial glycoconjugate antigen. mice were immunized with the glycoconjugate to prepare chlamydia-specific monoclonal antibodies. they were selected with chlamydia-specific lps antigens and ... | 1992 | 1372290 |
| characteristics of three vaginal flora patterns assessed by gram stain among pregnant women. vaginal infections and prematurity study group. | this study was undertaken to define the characteristics and persistence of vaginal flora in 7918 pregnant women at 23 to 26 weeks' gestation. vaginal smears were categorized as normal (predominant lactobacilli), intermediate (reduced lactobacilli), or positive for bacterial vaginosis. the women with normal flora were least likely to have elevated vaginal ph, amine odor, milky discharge, or colonization by gardnerella, bacteroides, or genital mycoplasmas. women with intermediate vaginal flora had ... | 1992 | 1372474 |
| chlamydial rrna in the joints of patients with chlamydia-induced arthritis and undifferentiated arthritis. | synovial fluid and synovial membrane specimens of 11 patients with chlamydia-induced arthritis (cia), 24 patients with undifferentiated arthritis (unda), 4 patients with post-enteritic reactive arthritis, 3 patients with lyme arthritis and 9 patients with rheumatoid arthritis were investigated for the presence of chlamydia trachomatis (c. trachomatis). a single stranded dna-probe was used for nucleic acid hybridization with ribosomal rna (rrna) from c. trachomatis. in 4 patients (cia = 1, unda = ... | 1992 | 1372544 |
| demonstration of chlamydial rna and dna during a culture-negative state. | trachoma is a common blinding disease of humans caused by ocular infections with chlamydia trachomatis. the cynomolgus monkey is a valuable primate model for the detection, pathobiology, and treatment of this infection. we have used this model system to compare the relative ability of tissue culture, direct fluorescence cytology, a modified polymerase chain reaction, and rna blotting to detect c. trachomatis following primary infection and reinfection over 34 weeks. six cynomolgus monkeys were g ... | 1992 | 1373404 |
| identification of chlamydia pneumoniae by dna amplification of the 16s rrna gene. | chlamydia pneumoniae is an important cause of respiratory disease in humans, but diagnosis of c. pneumoniae is hindered by difficulties in the in vitro growth of the organism. in order to improve detection and identification, we recently developed a polymerase chain reaction (pcr) assay which uses oligonucleotide primers specific for c. pneumoniae. the nucleic acid sequence was determined for the 16s rrna of c. pneumoniae, and regions in which c. pneumoniae differed from both chlamydia psittaci ... | 1992 | 1374077 |
| molecular evidence for the presence of chlamydia in the synovium of patients with reiter's syndrome. | there is much evidence indicating that chlamydial antigens in the synovium may be critical in the pathogenesis of reiter's syndrome (rs), but it is not known whether intact organisms are present in that tissue in any stage of the disease. the present study was undertaken to begin to address this question. | 1992 | 1374250 |
| immunoelectron microscopy of chlamydia psittaci with monoclonal antibodies. | an immunoelectron microscopic study was performed to determine the distribution of antigenic components on particles of chlamydia psittaci and infected cells using a number of monoclonal antibodies (mabs). of three anti-lipopolysaccharide (lps) antibodies (4d5, a2 and 4g5), two antibodies (4d5 and a2) reacted with the surface of reticulate bodies (rbs) but not with that of elementary bodies (ebs). the other antibody (4g5) reacted with both ebs and rbs. examination of infected cells in thin secti ... | 1992 | 1374830 |
| [reactive arthritis: pathogenesis, diagnosis and therapy]. | | 1992 | 1374987 |
| inapparent ocular infection by chlamydia trachomatis in experimental and human trachoma. | there is substantial indirect evidence which suggests that chlamydia trachomatis can generate inapparent, persistent infections in human. to confirm this directly, we examined ocular chlamydial infection in both the cynomolgus monkey model of trachoma and in patient samples from a trachoma-endemic area. in monkeys, ocular infection was studied over time using direct immunofluorescence cytology (dfa) and a molecular hybridization screening system which targets chlamydial ribosomal rna. in eleven ... | 1992 | 1375138 |
| monoclonal antibodies define genus-specific, species-specific, and cross-reactive epitopes of the chlamydial 60-kilodalton heat shock protein (hsp60): specific immunodetection and purification of chlamydial hsp60. | ocular and urogenital tract infections with chlamydia trachomatis can progress to chronic inflammatory diseases that produce blindness and tubal infertility. the pathophysiology of these chronic disease conditions is thought to be immunologically mediated, and the chlamydial 60-kda heat shock protein (hsp60) has been implicated as a major target antigen that stimulates the immunopathological response. the lack of chlamydial hsp60 antibodies and purified hsp60 has severely restricted studies to d ... | 1992 | 1375196 |
| unusual prevalence of the rare serovar da of chlamydia trachomatis in greece detected by monoclonal antibodies. | twenty-seven monoclonal antibodies (mab), eight with subclass-specificities and nineteen reacting with one or two c. trachomatis serovars, were developed and used to immunotype twenty-six clinical isolates from greek patients with chlamydial infections. twelve samples, first classified as serovar d, were identified as the recently established serovar da on the basis of their negative reaction with mab jg9, an mab previously shown to distinguish between d and da, and a new mab 114d9 with similar ... | 1992 | 1375784 |
| detection and antigenicity of chlamydial proteins that bind eukaryotic cell membrane proteins. | chlamydia psittaci proteins capable of binding eukaryotic cell membranes were identified and antigenically characterized. cell membrane proteins (cmp) of noninfected cells were labeled with biotin (b-cmp), then were extracted with 1% triton x-100. nitrocellulose membrane strips containing sodium dodecyl sulfate-polyacrylamide gel electrophoresis-resolved proteins of chlamydial elementary bodies (eb) were reacted with the b-cmp extract, followed by addition of streptavidin-conjugated horse radish ... | 1992 | 1378251 |
| clarithromycin. a review of its antimicrobial activity, pharmacokinetic properties and therapeutic potential. | clarithromycin is an acid-stable orally administered macrolide antimicrobial drug, structurally related to erythromycin. it has a broad spectrum of antimicrobial activity, similar to that of erythromycin and inhibits a range of gram-positive and gram-negative organisms, atypical pathogens and some anaerobes. significantly, clarithromycin demonstrates greater in vitro activity than erythromycin against certain pathogens including bacteroides melaninogenicus, chlamydia pneumoniae, chlamydia tracho ... | 1992 | 1379907 |
| keratitis in free-ranging koalas (phascolarctos cinereus) on magnetic island, townsville. | seventy free-ranging koalas (phascolarctos cinereus) from magnetic island (queensland, australia) underwent an ocular examination, blood collection and serological examination for chlamydia psittaci antibodies, and an examination of their teeth and genitalia. in 12 koalas long-standing unilateral keratitis was noted and in another 10 animals long-standing bilateral keratitis was observed. all animals were seronegative for chlamydia psittaci and apart from some nasal discharge and a variety of as ... | 1992 | 1380994 |
| fk-506-binding proteins from streptomycetes producing immunosuppressive macrolactones of the fk-506 type. | fk-506-binding proteins (fkbps), which in t cells are supposed to mediate the immunosuppressive effects of the compounds fk-506 and rapamycin, have been isolated from streptomyces chrysomallus, s. hygroscopicus subsp. ascomyceticus, and s. hygroscopicus. the latter two strains are producers of ascomycin (the ethyl analog of fk-506) and rapamycin, respectively. like the 12-kda fkbp in eukaryotic organisms such as humans, bovines, and saccharomyces cerevisiae, or the fkbps from gram-positive strep ... | 1992 | 1381710 |
| effective treatment of urethritis. a practical guide. | most cases of urethritis can be readily treated using recommended regimens. the most important causes of urethritis are chlamydia trachomatis and neisseria gonorrhoeae, and initial treatment is directed at them. optimal management requires obtaining a thorough sexual history, evaluation for objective clinical and laboratory evidence of infection, antimicrobial therapy directed towards the major aetiologies, and evaluation and treatment of sexual partners. treatment of gonorrhoea requires a singl ... | 1992 | 1382015 |
| arg276 of gsea, a chlamydia trachomatis kdo transferase, is required for the synthesis of the chlamydial genus-specific epitope in escherichia coli. | gsea is an enzyme from chlamydia trachomatis that can catalyse the addition of three 3-deoxy-d-manno-octulosonic acid (kdo) residues onto lipid a precursors. gsea is similar, and in a few stretches identical, in its amino acid sequence to kdta, an escherichia coli kdo transferase. in this study we altered an amino acid of gsea in a region that is identical between gsea and kdta to test its importance in the structure or catalytic activity of gsea. we found that when arg276 was changed to lys, il ... | 1992 | 1382035 |
| a novel 3-deoxy-d-manno-octulosonic acid transferase from chlamydia trachomatis required for expression of the genus-specific epitope. | dna cloned from chlamydia trachomatis is able to direct the formation of the genus-specific lipopolysaccharide epitope of chlamydiae in enteric gram-negative bacteria. we now demonstrate that a single c. trachomatis gene (gsea) is sufficient to impart this trait to escherichia coli. the deduced amino acid sequence of gsea shows 23% identity (66% similarity) to kdta, an e. coli gene that codes for a bifunctional enzyme catalyzing the addition of two 3-deoxy-d-manno-octulosonic acid (kdo) residues ... | 1992 | 1382060 |
| the role of lipopolysaccharide in the exposure of protective antigenic sites on the major outer membrane protein of chlamydia trachomatis. | a species-specific monoclonal igm antibody (mab) 9bf8 directed against the major outer membrane protein (momp) of chlamydia trachomatis neutralized several chlamydial serovars in a complement-independent manner. the presence of mg2+ ions negated the neutralization in serovars f, l1 and l2, but not in serovars a, b, e, d and k. the ability of monovalent fab-fragments of this mab to neutralize chlamydial infectivity in a mg-independent manner suggested that conformational alterations on the chlamy ... | 1992 | 1382112 |
| a nuclear magnetic resonance spectroscopic investigation of kdo-containing oligosaccharides related to the genus-specific epitope of chlamydia lipopolysaccharides. | the 1h- and 13c-nmr parameters, chemical shifts and coupling constants, for the pentasaccharide of the genus-specific epitope of chlamydia lipopolysaccharide and related di-, tri-, and tetra-saccharides have been measured and assigned completely using 1d and 2d techniques, and their structures have been confirmed. noe experiments indicated the preferred conformation of the pentasaccharide and the component oligosaccharides. the 3jh,h demonstrate a change in conformation by rotation of the c-6-c- ... | 1992 | 1382853 |
| [development of serodiagnostic kit "hitazyme chlamydia ab" for chlamydia trachomatis infections using extracted antigen]. | to develop eia kit with low cross-reactivity for the quantitative detection of anti-chlamydial antibodies, we examined the preparation of trachomatis antigens and its specificity to mouse antisera and human sera. the chlamydial elementary body (eb) purified from c. trachomatis l2/434/bu strain was treated by sarkosyl, dithiothreitol and sds by turns to obtain the soluble eb outer membrane (comc). sds-page showed that the major components of the comc were 96k, 60k and 39.5 kda peptides. the react ... | 1992 | 1383356 |
| seroepidemiologic study of hepatitis c virus in sexually transmitted disease risk groups. | to identify the importance of heterosexual activity as a possible route for the transmission of the hepatitis c virus (hcv), a screening of antibodies against hcv (anti-hcv) was performed in 200 sexually transmitted disease patients with different risks for incurring genital infections as well as in 100 registered prostitutes. out of all 300 persons tested, 14 cases of hcv infection were detected. anti-hcv was present in 3 of the prostitutes and in 11 of the std patients. evaluating known risk f ... | 1992 | 1384151 |
| [asymptomatic carriage of chlamydia trachomatis in men consulting for condylomata acuminata]. | a search for chlamydia trachomatis by cell culture was carried out in the urethra of 82 male patients consulting for condyloma acuminata at the clinical and biological centre for sexually transmissible diseases of the saint-louis hospital, paris. three patients had discreet urethral signs, but none had urethral discharge. cell culture was positive for c. trachomatis in 36 of the 82 patients (44 percent). this high prevalence suggests that c. trachomatis should systematically be looked for in the ... | 1992 | 1387948 |
| eukaryotic cells grown on microcarrier beads offer a cost-efficient way to propagate chlamydia trachomatis. | the use of microcarrier cell culture as a method for the in vitro propagation of the obligate intracellular bacterial parasite, chlamydia trachomatis, is described. the microcarrier beads proved to be a more cost-effective means to propagate c. trachomatis than traditional tissue culture flasks or roller bottles without sacrificing yields or infectivity. in addition, microcarrier cell culture was found to be a much simpler technique to study the intracellular development of these bacteria. | 1992 | 1389171 |
| chlamydia trachomatis antigen detection by chlamydiazyme combined with chlamydia blocking reagent verification. | several options exist for the detection of chlamydial infection in a routine laboratory setting. enzyme immuno assay (eia) technology offers rapid turn around of results and is less technically demanding than chlamydial cell culture. in addition, recently introduced eia confirmatory reagents have the potential to improve the accuracy of eia detection. we have evaluated one such confirmatory reagent (chlamydia blocking reagent, abbott laboratories) to determine the accuracy of the chlamydiazyme e ... | 1992 | 1391063 |
| pelvic inflammatory disease. | pelvic inflammatory disease is a common serious complication of the sexually transmitted pathogens neisseria gonorrhoeae and chlamydia trachomatis. there are more than 800,000 cases of pelvic inflammatory disease annually accounting for approximately 200,000 hospital admissions for acute and chronic infections. early accurate diagnosis and treatment are essential to prevent the serious sequelae including ectopic pregnancy, tubal disease infertility, chronic pain, and disability requiring multipl ... | 1992 | 1391639 |
| chlamydia trachomatis peritonitis: report of a patient presenting spontaneous regression of ascites. | a 36-year-old japanese woman complained of right hypochondralgia followed by ascites. paracentesis showed a turbid, straw-colored sterile exudate. computed tomography and magnetic resonance imaging of the abdomen revealed a left periuteric mass and ascites. the mass and ascites spontaneously regressed within a month with no specific treatment. later, after the patient had been discharged from hospital, immunofluorescence antibody titers for chlamydia trachomatis were successfully determined usin ... | 1992 | 1392190 |
| [detection of chlamydia trachomatis in clinical material 1989-1990]. | examination of 276 smears and scrapings from the uterine cervix of women before delivery and during the puerperium revealed in 28.0% positive findings. perinatal infection was confirmed by the presence of chlamydia trachomatis in 35.5% neonates with conjunctivitis and 27.3% positive smears from the nasopharynx. the authors used the direct immunofluorescence method. for immunofluorescent staining they used chlamyset of orion co., finland. | 1992 | 1394476 |
| chlamydia-induced arthritis. immunofluorescent antibody studies of the synovial fluid from 4 patients. | | 1992 | 1395231 |
| prevalence of chlamydia pneumoniae antibodies in hungary. | investigation of chlamydia pneumoniae (twar) antibodies in paired sera of 120 patients with various respiratory diseases revealed a prevalence of 4.2% of igg seroconversion. igg antibody without seroconversion was found in 83.3%. sera of ten patients showed titers as high as 512-1024 or above. children with no respiratory disease and blood donors in budapest had specific igg in 46.5% and 75.2% respectively. prevalence of igg antibody in children from the rural areas of hungary was about 50% lowe ... | 1992 | 1396727 |
| outbreak of chlamydia pneumoniae infection in four farm families. | the epidemiology of chlamydia pneumoniae infection was studied in an outbreak in four farm families living close together in denmark. eleven of 20 members of the families studied had bronchitis or pneumonia characteristic of chlamydia pneumoniae infection. serologic evidence of chlamydia pneumoniae as causative agent was strengthened by a high incidence of epidemic infection. transmission within families and a high frequency of disease versus asymptomatic infection are two findings which deviate ... | 1992 | 1396769 |
| detection of chlamydia trachomatis by the polymerase chain reaction in young patients with acute epididymitis. | specimens from 11 patients presenting with acute epididymitis were tested for the presence of chlamydia trachomatis by an enzyme immunoassay (eia), growth in mccoy cells and the polymerase chain reaction (pcr), and for other microorganisms by standard laboratory techniques. chlamydia trachomatis urethral infection was detected in four patients by tissue culture, in three patients by eia and in nine patients by pcr. these findings confirm the usually low detection rate of chlamydia trachomatis by ... | 1992 | 1396770 |
| chlamydial endocarditis: a report on ten cases. | over the period 1983-1990, 10 cases of infective native-valve endocarditis as a result of chlamydia were seen. all patients were men, with a mean age of 42 years, and none had a history of exposure to chlamydia psittaci. symptoms, such as weight loss and anorexia, with fever in eight cases, had persisted for at least 2 months before admission. haemodynamic failure was present in seven patients, and neurological signs in four. the aortic valve was involved in seven cases, the mitral valve in one ... | 1992 | 1396819 |
| a study of the incidence of urogenital chlamydia trachomatis in patients attending specialized departments of rome, milan and turin, italy. | a study of chlamydia trachomatis was conducted in 5270 subjects seen at clinics of the faculty of medicine and surgery at la sapienza university in rome, the s. anna hospital in turin, and the provincial maternity hospital institute in milan. in these areas, c. trachomatis was present in 5.8% of the cases examined; in addition it was present with statistically significant frequencies in cases of salpingitis (49.1) and epididymitis (21.7). it may also be found in cases of extrauterine pregnancy, ... | 1992 | 1397232 |
| inflammation on the cervical papanicolaou smear: the predictive value for infection in asymptomatic women. | the clinical significance of inflammation on the cervical papanicolaou (pap) smear of asymptomatic women is unknown. this study assessed the possible association between inflammation on pap smears with the presence of cervical/vaginal pathogens. | 1992 | 1397815 |
| complete sequence of the major outer membrane protein-encoding gene of chlamydia trachomatis serovar da. | the complete nucleotide sequence of the gene omp1 encoding the major outer membrane protein (momp) of chlamydia trachomatis serovar da was determined following amplification by the polymerase chain reaction. the most closely related complete sequence published to date is that encoding the c. trachomatis l1 momp. when the l1 and da momp deduced amino acid (aa) sequences were compared, 16 single-aa differences located mostly in the variable domains i, ii, and iv were detected. these regions contai ... | 1992 | 1398119 |
| the sequence of the gene encoding elongation factor tu from chlamydia trachomatis compared with those of other organisms. | nucleotide (nt) sequences encoding the elongation factor tu (ef-tu), trna(thr) and trna(trp) from chlamydia trachomatis have been determined. the environment of the ef-tu-encoding gene (tuf), between two trna gene sequences, suggests that it is part of a tufb locus. the nt sequence and the deduced amino acid (aa) sequence were aligned with comparable sequences from other organisms and the resulting data bases were used to infer phylogenies. phylogenetic trees based on aa sequences and nt sequenc ... | 1992 | 1398121 |
| chlamydia trachomatis and oral contraceptive use: a quantitative review. | chlamydia trachomatis is now recognised as a major sexually transmitted disease; oral contraceptive use is rapidly increasing particularly in developing countries. there are thus important public health implications of the many reports that isolation of c trachomatis is more frequent among users of oral contraceptives. the aim of this analysis was to assess the strength and consistency of this association by summarising published studies between 1972 and 1990. | 1992 | 1398654 |
| factors associated with genital chlamydial and gonococcal infection in females. | predictors of chlamydia and gonorrhoea can be used to increase the cost-effectiveness and acceptability of screening programmes, and allow targeting of control strategies. | 1992 | 1398655 |
| seroepidemiological and socioeconomic studies of genital chlamydial infection in ethiopian women. | to measure the prevalence of chlamydial genital infection in ethiopian women attending gynaecological, obstetric and family planning clinics; to identify the epidemiological, social and economic factors affecting the prevalence of infection in a country where routine laboratory culture and serological tests for chlamydial species are unavailable; to determine the risk factors for genital chlamydial infection in those with serological evidence of other sexually transmitted diseases. | 1992 | 1398656 |
| [ambulant atypical pneumonia: clinical diagnosis and therapy]. | atypical pneumonias are caused by mycoplasma, chlamydia and legionella species. from a clinical point of view the disease is comparable to influenza-like infections. macrolide antibiotics covered the species which are most important. especially new macrolides displayed better pharmacokinetic properties, allowing dose reduction and reduced frequency of intake. for legionella infections also therapy with modern quinolones is possible. | 1992 | 1398735 |
| effect of gamma interferon on resolution of murine chlamydial genital infection. | mice infected in the genital tract with the chlamydia trachomatis agent of mouse pneumonitis were treated with monoclonal rat anti-gamma interferon (anti-ifn-gamma) antibody to determine whether ifn-gamma participated in the resolution of the infection. in two experiments, anti-ifn-gamma antibody treatment resulted in significantly prolonged infections. in support of these data, passive administration of recombinant ifn-gamma to chronically infected nu/nu mice was able to bring about resolution ... | 1992 | 1398955 |
| staining of surface antigens of chlamydia trachomatis l2 in tissue culture. | surface labeling of chlamydial elementary and reticulate bodies in l929 cells infected with chlamydia trachomatis serotype l2 was monitored by using monoclonal antibodies (mab) against the major outer membrane protein and lipopolysaccharide (lps). different staining and fixation procedures were used to detect these surface antigens during the developmental cycle. anti-major outer membrane protein mab yielded a clear staining pattern of exclusively chlamydial inclusions independent of the fixatio ... | 1992 | 1398957 |
| identification of chlamydia trachomatis antigens by use of murine t-cell lines. | chlamydia-specific short-term t-cell lines were used in conjunction with immunoblot techniques to examine chlamydia trachomatis proteins for t-cell-stimulatory activity. this study was undertaken because of the known role of t cells in the resolution and pathogenesis of chlamydial infections. therefore, determination of which chlamydial proteins are t-cell antigens and whether they evoke protective immunity or contribute to immunopathology is crucial. immune lymph node cells were stimulated with ... | 1992 | 1398973 |
| prevalence of chlamydia psittaci infection among persons who work with birds. | | 1992 | 1399508 |
| disposition of roxithromycin in the epididymis after repeated oral administration. | the epididymal penetration of roxithromycin was studied in order to evaluate the drug for use in the treatment of epididymo-orchitis. seventeen patients hospitalized for surgery as part of treatment for prostatic adenoma or prostatic cancer were premedicated orally with roxithromycin 150 mg bd for three days followed by 150 mg pre-operatively (3 h before surgical incision). roxithromycin concentrations in serum and epididymis were determined by microbiological assay. the mean epididymal concentr ... | 1992 | 1399928 |