| prostaglandin i2 synthase in the lymphatic endothelium of rat liver as revealed by preembedding immunoelectron microscopy. | localization of prostaglandin i2 synthase-like immunoreactivity (pgi2si) in the lymphatic endothelium of rat liver was investigated by means of preembedding immunoelectron microscopy. vesicular and multivesicular bodies with pgi2si were closely apposed to golgi complexes of the endothelial cells. membrane-bounded pgi2si structures including vesicular and dense bodies were also present beneath the endothelial plasmalemma on both luminal and abluminal sides. other organelles within the endothelial ... | 1989 | 2657228 |
| cytoskeletal proteins including myofilaments in human tumors. | | 1989 | 2660368 |
| [the occurrence and significance of chlamydia psittaci and coxiella burnetii in dogs and cats. a study of the literature]. | with this review of the literature it is demonstrated that not only infections with chl. psittaci and c. burnetii do occur in dogs and cats in the federal republic of germany but also clinical diseases caused by these agents may occur. especially in diseases with uncharacteristic symptoms both bacteria have to be taken into consideration as causative agents. since both agents cause zoonotic infections a correct diagnosis is of special importance, especially if the owner of a diseased dog or cat ... | 1989 | 2662958 |
| zidovudine-induced fever. | zidovudine (3'-azido-3'deoxythymidine, azt, retrovir) is the first antiretroviral drug to demonstrate clinical efficacy for symptomatic human immunodeficiency virus infection. in a large, placebo-controlled trial, nausea and hematologic toxicity, but not fever, occurred more frequently in zidovudine- than in placebo-treated patients. however, in an open label study, fever severe enough to halt zidovudine administration occurred in 10% of 70 acquired immune deficiency syndrome (aids) patients rec ... | 1989 | 2666640 |
| biologically active casein peptides implicated in immunomodulation. | maternal milk should not only be considered as a nutrient, but also as a protecting agent against aggressions from the neonate's new environment. breast-feeding facilitates transmission of a passive immunity by multifunctional factors which have a direct effect on the neonate's resistance to bacterial and viral infections. among these factors are the main milk proteins, the caseins: during enzymic digestion of human and bovine caseins, immunomodulating peptides are released. corresponding synthe ... | 1989 | 2668358 |
| titration of coxiella burnetii in buffalo green monkey (bgm) cell cultures. | titration of coxiella burnetii, strain nine mile, phase i, and strain frankfurt, phase ii, in buffalo green monkey (bgm) cover slip cell cultures yielded reproducible infectivity titers after centrifugation of infected cell cultures (3000 g, 30 min, 37 degrees c) and a subsequent 4-day incubation at 37 degrees c in minimal essential medium (mem). compared to other titration procedures (plaque assays, titration in embryonated chicken eggs), this technique proved to be fast, less laborious and eco ... | 1989 | 2669791 |
| interleukin-1 potentiates granulopoiesis and thrombopoiesis by producing hematopoietic factors in vivo. | in vivo administration of recombinant human interleukin-1 beta (rhu il-1 beta) selectively enhanced the recovery from granulocytopenia and thrombocytopenia caused by whole body irradiation, in a dose dependent manner. since il-1 itself in vitro had no colony-stimulating activity (csa), we studied whether il-1 can produce hematopoietic factors in vivo, which in turn will promote granulopoiesis and thrombopoiesis. serum from il-1 injected mice showed marked granulocyte/macrophage csa (gm-csa), but ... | 1989 | 2671565 |
| [the gray rat (rattus norvegicus) as a carrier of infectious causative agents in siberia and the far east]. | literary data and antiplague institutions' reports demonstrate that norway rats carry over 24 nosological forms and groups of infectious diseases: plague, tularemia, pseudotuberculosis, intestinal yersiniosis, salmonellosis, erysipeloid, listeriosis, leptospirosis, pasteurellosis, brucellosis, dysentery, paratuberculosis, hemorrhagic nephroso-nephritis, omsk hemorrhagic fever and q fever, tick-borne and japanese encephalitis, lymphocytic choriomeningitis, tick-borne rickettsiosis and rickettsial ... | 1989 | 2674644 |
| [q fever in guinea-bissau. 1 case]. | q fever is seldom reported in west africa. the case of a man returning from guinea bissau who presented an acute lobar pneumonia with fever, headache, hematuria and hepatitis was confirmed by high titers of antibody in phase ii indirect immuno-fluorescence which appeared on the twelfth day of fever. treatment with erythromycin was continued by doxycyclin, and complete resolution of all signs was promptly obtained. coxiella burnetii might thus be responsible of cases of unexplained fever with res ... | 1989 | 2680135 |
| [tolerance and reverse tolerance of nicotine on spontaneous motor activity]. | repeated administration of nicotine induces tolerance and/or reverse tolerance of spontaneous motor activity in rodents. these effects of nicotine on the spontaneous motor activity have been shown to be influenced by drug factors (e.g., doses, elapsed time after injection, period of treatment), circadian rhythm, age, sex, strain and species of animals. although nicotinic acetylcholine receptors in the brain participate in the effects of nicotine, the development of tolerance and reverse toleranc ... | 1989 | 2680832 |
| interrelationships between digestive proteolytic activities and production and quantitation of toxins in pseudomembranous colitis induced by clostridium difficile in gnotobiotic mice. | clostridium difficile pathogenicity is related to in vivo production of toxins, and it is of great interest to detect toxins produced in biological samples. several reports have shown that proteases in stools interfere with immunological methods for quantitation of toxin a. the purpose of this work was to estimate the relationship between the proteases and the c. difficile toxins produced in a gnotobiotic mouse model of pseudomembranous cecitis. cecal proteolytic activities hydrolyzed toxin a, a ... | 1989 | 2680988 |
| [lesions of the cardiovascular system in q fever]. | | 1989 | 2681990 |
| phosphoroselenoate oligodeoxynucleotides: synthesis, physico-chemical characterization, anti-sense inhibitory properties and anti-hiv activity. | oligodeoxynucleotides with a phosphorus atom in which one of the non-bridging oxygen atoms is substituted by selenium were prepared and investigated with respect to their antisense properties. a general synthesis of phosphoroselenoate analogs of oligonucleotides is described using potassium selenocyanate as the selenium donor. the compounds, characterized by 31p nmr, were shown to decompose to phosphate with a half-life of ca. 30 days. melting temperatures of duplexes between poly(ra) or poly(ri ... | 1989 | 2682524 |
| [serologic studies of domestic animals for listeriosis, q-fever, and brucellosis in cairo]. | first serological investigations among domestic animals (donkey, goat, sheep, swine, dog) and rats (rattus norvegicus) carried out in the surroundings of persons from waste disposal sites in cairo indicate the occurrence of listeriosis, q fever and brucellosis. | 1989 | 2684762 |
| [the phase variation of coxiella burnetii (theory and practice)]. | | 1989 | 2686315 |
| animal health research in the small ruminant collaborative research support program. | disease is a major constraint in small ruminant production systems in lesser-developed countries throughout the world. animal health projects have been an integral part of the small ruminant collaborative research support program (sr-crsp) from its inception. at the onset, these projects were oriented toward herd health care and veterinary extension activities. later, all the projects developed a sharper focus in that they were directed to more basic studies of infectious disease. diseases curre ... | 1989 | 2687219 |
| hydrophobic grid membrane filter/mug method for total coliform and escherichia coli enumeration in foods: collaborative study. | twenty-four laboratories participated in a collaborative study to validate a hydrophobic grid membrane filter (hgmf) method incorporating the use of 4-methylumbelliferyl-beta-d-glucuronide (mug) for enumeration of total coliform and escherichia coli bacteria in foods by comparing its performance against the aoac 3-tube mpn method (46.013-46.016). raw milk, raw ground poultry, whole egg powder, cheese powder, and ground black pepper were included in the study. the total coliform methods did not d ... | 1989 | 2687225 |
| isolation of constitutive mutations affecting the proline utilization pathway in saccharomyces cerevisiae and molecular analysis of the put3 transcriptional activator. | the enzymes of the proline utilization pathway (the products of the put1 and put2 genes) in saccharomyces cerevisiae are coordinately regulated by proline and the put3 transcriptional activator. to learn more about the control of this pathway, constitutive mutations in put3 as well as in other regulators were sought. a scheme using a gene fusion between put1 (s. cerevisiae proline oxidase) and galk (escherichia coli galactokinase) was developed to select directly for constitutive mutations affec ... | 1989 | 2689861 |
| clinical aspects and prevention of q fever in animals. | the natural reservoir of coxiella burnetii encompasses many free-living vertebrates but the major risk of human infection arises through contact with infected ruminant livestock and their contaminated products. the organism has a remarkable affinity for the ruminant placenta and mammary gland but the great majority of naturally-occurring infections are asymptomatic. however, the potential of c. burnetii to cause abortion has been demonstrated experimentally and observed in the field while more r ... | 1989 | 2691272 |
| [treatment of pneumonia caused by legionella, mycoplasma, chlamydiae and rickettsia using ofloxacin]. | to asses the efficacy and the safety of ofloxacin as therapy of pneumonia caused by intracellular pathogens, 35 patients were studied (26 male, 9 female, mean age: 52.5 +/- 16.6 years). causative pathogens were chlamydia psittaci (n = 13), legionella pneumophila (n = 10), mycoplasma pneumoniae (n = 7) and coxiella burnetii (n = 5). ofloxacin was administered orally in 32 cases (200 mg b.i.d. in 80% of cases) and by i.v. route in 3 cases. all patients were cured without any side effects. in concl ... | 1989 | 2691969 |
| [null alleles of yeast sup1 and sup2 genes: interaction with cytoplasmic determinants]. | | 1989 | 2693034 |
| [the biological properties of rickettsia burnetii isolated in the northwestern ukrainian ssr]. | biological properties of two strains of the burnet rickettsia isolated in the natural foci of the q fever in the northwest of the ukrainian ssr are studied. strain "gishin" was characterized by high virulence for the laboratory animals and considerably exceeded the strain "politsa" in the virulent properties. antigen of phase i prepared from the strain "politsa" was highly active in revealing the corresponding antibodies. the high-sensitive reaction of indirect immunofluorescence has shown the p ... | 1989 | 2695792 |
| effect of sodium artesunate on malaria infected human erythrocytes. | oxidative stress in malaria infected human erythrocytes is augmented and the anti-oxidant system is attenuated as compared with normal rbc's. exacerbation of intra-erythrocytic oxidative stress might provide a means to kill the parasites. sodium artesunate (sa), an effective chinese anti-malaria drug, markedly increased the levels of active oxygen species and production of malonyldialdehyde in normal red blood cells and, to a greater extent, in malaria infected red blood cells. sa caused a remar ... | 1989 | 2698476 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| streptococcus pneumoniae endocarditis presenting as an epidural abscess. | streptococcus pneumoniae is an important pathogen in many serious infections, such as pneumonia, sinusitis, and meningitis. it uncommonly causes infective endocarditis and only rarely produces spinal epidural abscesses. this report describes a patient who had pneumococcal infection presenting as an acute epidural abscess and who was subsequently found to have endocarditis. s. pneumoniae was cultured from both blood and abscess material. a high level of clinical suspicion of endocarditis is impor ... | 1989 | 2704929 |
| prenatal ontogenesis of pro-opiomelanocortin in the mouse central nervous system and pituitary gland: an in situ hybridization and immunocytochemical study. | pro-opiomelanocortin (pomc) mrna detected by in situ hybridization and pomc/acth (adrenocorticotropin)-containing neurons detected by immunocytochemistry were first observed in the presumptive arcuate nucleus of embryonic mouse brain on gestational day 10.5 (e10.5). immunostained fibers were also evident on e10.5 in the lateral and dorsal diencephalon. in these areas, a dense network of processes developed by e11.5 and extended into the mesencephalon. fibers were detected in the myelencephalon a ... | 1989 | 2706773 |
| q fever pneumonia. | pneumonia is one of several clinical syndromes that results from inhalation of coxiella burnetii. this microorganism, the etiologic agent of "q" (query) fever, infects a wide range of animals and insects. cattle, sheep, goats, and cats are the reservoirs whereby this agent is spread to humans. high concentrations of c burnetii are present in the placenta and at parturition, the organism is shed into the environment to be inhaled by humans. following an incubation period that ranges from four to ... | 1989 | 2711056 |
| effects of inducers of differentiation on protein kinase c and cmp-n-acetylneuraminic acid:lactosylceramide sialyltransferase activities of hl-60 leukemia cells. | exposure of hl-60 leukemia cells to either 12-o-tetradecanoylphorbol-13-acetate (tpa), dimethylsulfoxide (dmso), exogenous gangliosides gm3, gm1, or bovine brain ganglioside mixture (bbg) resulted in a marked inhibition of the growth of cells. the order of the inhibitory potency was tpa greater than gm3 greater than dmso greater than bbg greater than gm1. in contrast, sulfatides were without effect on cellular replication. treatment of hl-60 cells with tpa or gm3 induced differentiation along th ... | 1989 | 2715723 |
| [treatment of atypical pneumonia with josamycin]. | in the present study the clinical efficacy and tolerance of josamycin (1 g every 12 hours) was prospectively evaluated for the empirical therapy of patients below 70 years with community acquired pneumonia of atypical presentation (ap), without respiratory failure, radiological cavitation or risk factors of pharyngeal colonization by gram-negative bacilli. during a 28-month period 168 patients adequate for the study were included. the etiological diagnosis was established in 56 cases (33.3%); in ... | 1989 | 2716415 |
| osteoarticular infection due to coxiella burnetii. | | 1989 | 2723460 |
| the ability of mononuclear cells to coagulate blood in response to coxiella burnetii. | endocarditis is a rather frequent complication of q fever caused by coxiella burnetii. we examined the ability of phase i (virulent) or phase ii (avirulent) c. burnetii to coagulate blood in the presence of human blood mononuclear cells in vitro. after incubation for 4 h, virulent phase i c. burnetii was an effective stimulant for mononuclear cells. since this interaction is a potent trigger of blood coagulation through the extrinsic pathway, it could be responsible for the local deposition of f ... | 1989 | 2724327 |
| induction of experimental thyroid dysfunction in rats with implantable pellets of thyroxine or propylthiouracil. | subcutaneously implanted pellets containing the thyroid hormone thyroxine or the thyrotoxic agent propylthiouracil were used to induce hyper- or hypothyroidism in rats. the results obtained were compared to those produced by daily subcutaneous injection of these substances. the thyroxine pellets caused substantial elevation of serum thyroxine concentrations for at least 25 days, whereas the propylthiouracil pellets caused a pronounced decrease of serum thyroxine concentrations. changes in heart ... | 1989 | 2724991 |
| effects of perinatal exposure to aspartame on rat pups. | possible effects of perinatal exposure to l-aspartyl-l-phenylalanine methyl ester (aspartame) on rat pups were investigated. adult female rats, and later their pups, were exposed, via their drinking water, to aspartame (0.007%, 0.036%, 0.18% or 0.9% w/v) or phenylalanine (0.45% w/v) for 12 days prior to conception until the pups were 38 days old. control rats were given plain water. the adults exposed to aspartame consumed an average of 14, 68, 347 and 1614 mg/kg/day of aspartame and those expos ... | 1989 | 2725436 |
| prospects for a vaccine against hiv. | | 1989 | 2725655 |
| the correlation of electroretinographic and histopathological findings in the eyes of mice infected with the 79a strain of scrapie. | retinal degeneration was produced using the 79a strain of scrapie injected intracerebrally into mice of the mm inbred strain, and studied by electroretinography and light and electron microscopy during the preclinical and clinical phases of the disease. the changes in the electroretinogram (erg), detectable in some mice at 118 days postinjection (d.p.i.), appeared to coincide with the earliest morphological evidence of photoreceptor damage. in the early stages there was a progressive reduction o ... | 1989 | 2725831 |
| q fever pneumonia: a review of 164 community-acquired cases in the basque country. | one hundred and sixty four cases of q fever pneumonia are reviewed. coxiella burnetti is responsible for 18.8% of pneumonias acquired in the community in our region with an extremely high seasonal variation. 91% of the cases occur between january and june. 88.5% of the patients are less than 40 yrs of age and 77% are male. the most common clinical symptoms are high fever, cough, cephalalgia and myalgias. 46.5% of the patients have no respiratory symptoms although 34% of the cases report pleural ... | 1989 | 2731605 |
| interferon antibodies in patients with chronic hbv infection treated with recombinant interferon. | in different trials, a total of 46 adults and 12 children suffering from chronic hepatitis b virus (hbv) and who were hbv-dna- and hbeag-positive were treated with recombinant interferon-alpha (rifn alpha)-2a. the interferon was administered intramuscularly in different doses ranging from 1.5 mu to 20 mu/m2 of body surface, two or three times weekly during 4-6 months. specific detection of anti-ifn antibodies by enzymoimmunoassay (eia), radioimmunoassay (ria) and biological assays during treatme ... | 1989 | 2732449 |
| igg and igm responses in high and low antibody-selected lines of chickens. | kinetics of igg and igm as measured by 2-mercaptoethanol-resistant (mer) and susceptible (mes) antibodies to sheep erythrocytes, respectively, were determined as correlated responses in lines of chickens selected for high (ha) and low (la) antibody response to sheep erythrocytes. primary response patterns for total, mer, and mes antibody differed according to the genetic line. total antibody increased rapidly, peaked, and persisted at moderate levels in line ha, whereas both peak and persistency ... | 1989 | 2732455 |
| immunocytochemical localization of adrenodoxin in bovine adrenal cortex by protein a-gold technique. | to investigate the intracellular localization of the enzymes that are involved in steroid hormone synthesis, an immunocytochemical study of the distribution of adrenodoxin in cells of the bovine adrenal cortex was carried out by the post-embedding immunostaining method and by immuno-cryoultramicrotomy in combination with the protein a-gold technique. gold particles were seen on the matrix and the inner membrane of all the mitochondria examined, which have typical vesicular or tubulo-vesicular cr ... | 1989 | 2737927 |
| upstream regulatory elements of murine alpha 4-interferon gene confer inducibility and cell type-restricted expression. | we have identified and functionally characterized dna sequences that are required for the inducible and cell-restricted expression of the murine alpha 4-interferon gene. hybrid plasmids in which the alpha 4 promoter region or its 5' deletions were inserted upstream of the cat gene were constructed, and the expression of these hybrid genes was studied in mouse l-cells both in permanent and transient assays with comparable results. inducible expression was not affected by deletions up to -109; how ... | 1989 | 2738062 |
| alternative routes for the establishment of surface polarity during compaction of the mouse embryo. | during the process of compaction, mouse 8-cell blastomeres flatten upon each other and polarize along an axis perpendicular to cell contacts. if the process of flattening is prevented, polarization can still occur, but does so in a lower proportion of cells than for control populations, and without the normal contact-directed orientation. we compared contact-directed and noncontact-directed processes to see if they involve common mechanisms. in nonflattened cells, surface polarization was favore ... | 1989 | 2744237 |
| serological investigations of the hedgehog (erinaceus europaeus) in styria. 2. report. | from 1975 to 1982 investigations of 64 hedgehogs for anthropozoonoses and infectious diseases were carried out in all regions of styria, austria. the serological investigations revealed positive results against rickettsiosis, (q-fever and rmsf-groups antigen), (ornithosis, toxoplasmosis, listeriosis, against adenoviruses and herpesvirus). other studies by nosek et al. (1967); troger et al. (1976); kaaserer et al. (1976) and stunzner et al. (1976) report on the hedgehog (erinaceus europaeus) as i ... | 1989 | 2744467 |
| [incidence of coxiella burnetii and rickettsiae from the rmsf group in the sub-alpine region in austria]. | investigations were carried out on small mammals to determine further distribution of coxiella burnetii and rickettsia rmsf-group in the alpine and sub-alpine regions. in altitudes of 1,200-2,400 m antibodies against clethrionomys glareolus, apodemus flavicollis, sorex alpinus, sorex minutus and microtus agrestis were demonstrated. these findings emphasize that in the entire alpine and sub-alpine regions of austria the occurrence of c. burnetii and rmsf-group rickettsia is to be expected. | 1989 | 2744470 |
| serological investigations of small mammals in waste disposal sites in austria. 2nd report. | in serological investigations of small mammals in waste disposal sites, in austria, antibodies were demonstrated against q-fever, rmsf-rickettsia, listeriosis, tbe (fsme) and west-nile virus. | 1989 | 2744474 |
| susceptibility of coxiella burnetii to pefloxacin and ofloxacin in ovo and in persistently infected l929 cells. | the relative lack of efficacy of the antibiotic treatment of chronic q fever endocarditis justifies the further evaluation of the susceptibility of coxiella burnetii to the modern quinolone antibiotics. we evaluated the efficacies of pefloxacin and ofloxacin in controlling the nine mile isolate of c. burnetii by using an embryonated egg assay and persistently infected l929 cells in culture. pefloxacin was effective in controlling the intracellular parasite at a concentration of 50 micrograms per ... | 1989 | 2751278 |
| phylogenetic diversity of the rickettsiae. | small subunit rrna sequences have been determined for representative strains of six species of the family rickettsiaceae: rickettsia rickettsii, rickettsia prowazekii, rickettsia typhi, coxiella burnetii, ehrlichia risticii, and wolbachia persica. the relationships among these sequences and those of other eubacteria show that all members of the family rickettsiaceae belong to the so-called purple bacterial phylum. the three representatives of the genus rickettsia form a tight monophyletic cluste ... | 1989 | 2753854 |
| concomitant human infection due to rickettsia conorii and coxiella burnetii. | | 1989 | 2760493 |
| the establishment of a clinical diagnostic method of the visual evoked potentials (veps) in the cat: the effects of recording electrode positions, stimulus intensity and the level of anesthesia. | the establishment of characteristic wave pattern of visual evoked potentials (veps) in the cat was attempted in order to be aid for clinical veterinary practitioners with the evaluation of visual dysfunction. 1) the position where the largest response of the veps was detected was close to the midline of the occipital area in normal cats. 2) the veps consisted of three components with latencies within 100 msec after flash stimulus. 3) flash stimuli of higher illumination produced veps of shorter ... | 1989 | 2761147 |
| c-terminal peptide alcohol, acid and amide analogs of desulfato hirudin54-65 as antithrombin agents. | analogs of the antithrombin peptide hirudin54-65 with c-terminal modifications have been synthesized in order to examine the requirements for alpha-thrombin inhibition. the c-terminal residue, gln65, could be replaced with l-amino acids or amino alcohols with neutral or charged hydrophilic side chains without greatly affecting the peptide's antithrombin potency as determined by inhibition of thrombin-induced clot formation in human plasma in vitro. derivatives with d- or l-amino carboxamides at ... | 1989 | 2763270 |
| effects of idebenone on memory impairment induced in ischemic and embolization models of cerebrovascular disturbance in rats. | two rat models of memory impairment in passive avoidance learning induced by cerebrovascular disturbance, were established to estimate the effects of a cerebral metabolic enhancer, idebenone. transient and global cerebral ischemia in rats, produced by 4-vessel occlusion for 200 s immediately after the acquisition trial of passive avoidance learning, shortened the latencies in the retention test trial performed 24 h later. this retrograde amnesia was reversed significantly by idebenone administer ... | 1989 | 2764639 |
| morphologic and morphometric studies on tumor necrosis produced by radiotherapy, and hyperthermia singly and in combination. | the purpose of the present study was to evaluate the therapeutic effects of hyperthermia associated with radiotherapy on neoplasia. two transplanted experimental tumors (undifferentiated carcinoma of mouse breast and sarcoma 37) were used. a protocol was followed that included, for both models, four groups of animals: 1) control group; 2) radiotherapy group; 3) hyperthermia group; 4) radiotherapy associated with hyperthermia group. the animals were sacrificed after therapy according to a differe ... | 1989 | 2770309 |
| [influence of electric stimulation of the medulla oblongata on the smooth muscle tone of the airway in cats]. | changes in the tracheal smooth muscle tone have been studied in 30 cats anaesthetized with a chloralose-urethane mixture and paralyzed with succinyl choline bromide during electrical stimulation of the medulla oblongata. constrictor responses were evoked from the regions including the caudal portion of the dorsal motor nucleus of the vagus, nucleus ambiguous and neighbouring structures of the reticular formation. dilatory responses of the tracheal smooth muscle were observed during stimulation o ... | 1989 | 2770913 |
| [recurrent pericarditis with effusion caused by q fever. presentation of a case]. | we present a case report of recurrent pericarditis with pericardial effusion and high levels of coxiella burnetti antibodies (phase ii), excluding other possible etiologies. the rarity of this disorder is commented and the necessity of be considered this etiological possibility in a second time, particularly in patients who are susceptible to suffer q-fever disease. | 1989 | 2772370 |
| severe disease in a dairy herd associated with acute infection with bovine virus diarrhoea virus, leptospira harjo and coxiella burnetii. | between november 1984 and february 1985, a serious outbreak of pyrexia, diarrhoea, agalactia, metritis and severe weight loss affected most of the recently calved cows in a 183-cow dairy herd in norfolk. fifteen cows died and 20 were culled. forty cows aborted during or after the outbreak, and many of them produced mummified fetuses; 18 calves were stillborn and three others died soon after birth. necropsy of three affected cows revealed ulceration of the gastrointestinal tract similar to that s ... | 1989 | 2773203 |
| pericarditis associated with tick-borne q fever. | a case of pericarditis associated with q fever is described. transmission was probably via an arthropod vector, which was most likely the kangaroo tick amblyomma triguttatum. complete recovery occurred in association with the use of non-steroidal anti-inflammatory agents only. this is a rare presentation of q fever implicating transmission by a novel vector. | 1989 | 2775046 |
| the occurrence of hiv antibodies in drug addicts autopsied at the university institute of forensic medicine in copenhagen in 1987. | in 1987 a total of 89 intravenous drug addicts were admitted to medico-legal autopsy at the university institute of forensic medicine in copenhagen. totally 10 cases were reactive by the elisa test and five of these, or 7% of the total material tested, could be confirmed by the immuno-blotting test. the other five cases must be considered as false positive, possibly due to antibodies against cellular antigens. among the five real positive cases, four were known before-hand while in one case the ... | 1989 | 2777161 |
| repair of sublethal damage in two human tumor cell lines grown as multicellular spheroids. | multicellular tumor spheroids (mts) provide a suitable in vitro model to study radiation sensitivity of tumor cells. two cell lines of human origin, obtained from a neuroblastoma (nb-100) and a squamous cell carcinoma (hn-1), were exposed to graded doses (4-9 gy) of radiation with 18 mv photons. radiation was applied either as a single or as a split dose with an interval of 6 hr to determine the extent of sublethal damage repair. treated spheroids regrew at approximately the same growth rate as ... | 1989 | 2777647 |
| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | | 1989 | 2780954 |
| antibiotic susceptibilities of two coxiella burnetii isolates implicated in distinct clinical syndromes. | antibiotic susceptibility testing of two isolates of the q-fever agent, coxiella burnetii, was performed with recently and persistently infected l929 fibroblast cells. the two genetically distinct isolates, nine mile and priscilla, are implicated in two different clinical disease syndromes, acute and chronic q fever, respectively. we compared the efficacies of rifampin, doxycycline, and five 4-quinolone compounds (ciprofloxacin, difloxacin, ofloxacin, norfloxacin, and pefloxacin) in reducing per ... | 1989 | 2782856 |
| human tonsillar dendritic cell-induced t cell responses: analysis of molecular mechanisms using monoclonal antibodies. | dendritic cells, isolated from human tonsillar tissue, were found to be potent stimulators of the sodium periodate t cell oxidative mitogenesis reaction. monoclonal antibodies against cd2, cd4, cd11a, cd18, lfa-3, icam-1, class i and class ii major histocompatibility complex (mhc) inhibited t cell proliferation in this response, whereas antibodies against cd8, cd11b, cd11c and cd16 had no effect. further, antibodies against cd2, cd11a, cd18, lfa-3 and icam-1 inhibited the early dendritic cell-t ... | 1989 | 2786468 |
| non-suppurative bacterial arthritis diagnosed by fine-needle aspiration biopsy. | | 1989 | 2799306 |
| [incidence of coxiella burnetti and chlamydia psittaci in cattle in southern bohemia]. | in 1986 and 1987 in six districts and 22 localities of the southern bohemian region, and in one district and one locality of the western bohemian region the total of 747 head of cattle was examined by a microagglutination method (mar) and complement fixation method (kfr) for antibodies to coxiella burnetii and chlamydia psittaci. the positivity of c. burnetii detection was 44.4% in mar and 16.5% in kfr. the positivity of chl. psittaci detection was 7.1%. the widespread incidence and highly posit ... | 1989 | 2800248 |
| q fever urban cases in romania. | q fever urban sporadic cases in romania in the 1981-87 period are reviewed as concerns their incidence, seasonality and epidemiological data. urban cases represented 76.8% as against 23.2% rural cases from the 134 q fever sporadic cases detected in this period. cases were distributed throughout all months with peaks during april (18.4% of cases) and june (19.4%). infections were not related to contact with livestock or domestic birds or with raw milk drinking. cases could not be identified as q ... | 1989 | 2802967 |
| steroidogenesis in liposomal system containing adrenal microsomal cytochrome p-450 electron transfer components. | purified adrenal microsomal p-450c21 and/or p-45017 alpha,lyase were incorporated with purified nadph-cytochrome-p-450 reductase into liposome membranes composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine at a molar ratio of 5:3:1. the rate dependences of reduction of liposomal p-450c21 in the fast phase as well as progesterone hydroxylation activities of p-450c21 and p-45017 alpha,lyase on the reductase concentration in the liposome membranes suggested that electron ... | 1989 | 2804111 |
| [differentiated changes in the activity of the sympathetic nerves induced by glycopenia in spontaneously hypertensive rats]. | 2-deoxy-d-glucose (2-dg) evokes an increase in the efferent discharge rate of the adrenal nerve in anesthetized rats and rabbits. a 63% increase in adrenal sympathetic nerve activity (adrsna) occurred within 15 min after 125 mg/kg 2-dg administration in alert animals, no changes being revealed in the renal sympathetic nerve activity (rensna), blood pressure (bp) or the heart rate (hr). additional administration of 375 mg/kg 2-dg (cumulative dose 500 mg/kg) led to an over 120% increase in the adr ... | 1989 | 2806664 |
| endotoxicosis induced by coxiella burnetii lipopolysaccharide stimulates a ribosomal protein s6 kinase: some properties of the partially purified enzyme. | guinea pig endotoxicosis induced by lipopolysaccharide from coxiella burnetii nine mile phase i stimulates phosphorylation of liver ribosomal protein s6, with a 50% increase at 12 h postinoculation. the responsible protein kinase (s6pk) has been partially purified from liver; its activity is independent of cyclic amp and of ca2+ plus phosphatidyl serine or diacylglycerol. the preparation has an apparent optimum concentration of 20 mm mg2+, while ca2+ and mn2+ are each inhibitory at 2 mm. the app ... | 1989 | 2807543 |
| role of adenosine in the gastric blood flow response to pentagastrin in the rat. | we tested the hypothesis that the increase in gastric mucosal blood flow during pentagastrin-stimulated acid secretion in the rat is mediated partly by endogenously generated adenosine. in in vivo microscopic studies, topical 10(-5) to 10(-3) m adenosine dose-dependently dilated the submucosal arterioles, the vessels that control mucosal blood flow. the adenosine receptor antagonist 8-phenyltheophylline, significantly reduced adenosine's vasodilatory response. an adenosine analog with a high a2 ... | 1989 | 2810111 |
| role of adenosine in the gastric blood flow response to pentagastrin in the rat. | we tested the hypothesis that the increase in gastric mucosal blood flow during pentagastrin-stimulated acid secretion in the rat is mediated partly by endogenously generated adenosine. in in vivo microscopic studies, topical 10(-5) to 10(-3) m adenosine dose-dependently dilated the submucosal arterioles, the vessels that control mucosal blood flow. the adenosine receptor antagonist 8-phenyltheophylline, significantly reduced adenosine's vasodilatory response. an adenosine analog with a high a2 ... | 1989 | 2810111 |
| effects of cisapride on isolated guinea pig colon. | effects of cisapride were studied on the whole segments and muscle strips isolated from the guinea pig ascending colon. in whole segment preparations, external application of cisapride (0.4-10 microm) produced relaxation and inhibited spontaneous and evoked intraluminal pressure changes. intraluminal application (1-10 microm) increased the pressure response. in many circular muscle strips (18 preparations out of 20), cisapride (0.3-1 microm) increased the mechanical activity, particularly when t ... | 1989 | 2810941 |
| serological evidence of q fever in cattle in malawi. | the serological prevalence of coxiella burnetii in cattle in malawi is unknown. serum samples from 200 malawian zebu cattle were tested for c. burnetii antibodies using the complement fixation test. the percentage of positive and suspicious titres was 1.5% and 5% respectively. | 1989 | 2812706 |
| coxiella burnetii infection in prosthetic devices. | | 1989 | 2814166 |
| isolation of haemophilus somnus from bovine milk. | | 1989 | 2815520 |
| urolith analysis. | | 1989 | 2815527 |
| urolith analysis. | | 1989 | 2815527 |
| interstitial deletion of (17)(p11.2). a microdeletion syndrome. another example. | another example of del (17)(p11.2) in a 3-year-old boy with psychomotor retardation, broad face, midface hypoplasia, prognathism, and behavioural anomalies was diagnosed clinically and confirmed by prometaphase analysis. it seems that this new microdeletion syndrome may not be so rare. | 1989 | 2817780 |
| similarities and differences in the regulation of hepatic cytochrome p-450 enzymes by diabetes and fasting in male rats. | the effects of streptozotocin-induced diabetes and fasting on hepatic cytochrome p-450 enzymes in sexually mature male rats were studied by immunochemical techniques and enzyme assays. the level of cytochrome p-450ac (an acetone/ethanol inducible form), 65 pmol/mg microsomal protein in control rats, increased 4- to 5-fold in diabetic rats and 3- and 5-fold in fasting rats. in contrast, p-450 ut-a (a male specific form) decreased drastically from 295 pmol/mg in the control group to about 10% of t ... | 1989 | 2818618 |
| 'ring' granulomas in cytomegalovirus hepatitis. | a liver biopsy specimen obtained from a patient with cytomegalovirus hepatitis demonstrated "ring" granulomas similar to those previously described as characteristic of acute coxiella burnetii infection. q fever is not the only infectious disease in which fibrinoid rings may form within granulomatous foci. | 1987 | 2820347 |
| in vivo pharmacology of ici 198,615: a novel, potent and selective peptide leukotriene antagonist. | the in vivo pharmacology of ici 198,615, a highly potent and selective peptide leukotriene (lt) receptor antagonist, is described. in a conscious guinea pig model, ici 198,615 provided dose-related antagonism of aerosol ltd4-induced dyspnea when administered p.o., i.v. or by aerosol. the pharmacologic half-lives of ici 198,615 by these routes of administration were greater than 16 hr, 68 min and 34 min, respectively. in pulmonary mechanics studies in which the effects of i.v. or aerosol administ ... | 1987 | 2824752 |
| paraventricular nucleus lesion prevents yawning and penile erection induced by apomorphine and oxytocin but not by acth in rats. | the effect of electrolytic lesion of the paraventricular nucleus of the hypothalamus (pvn) on yawning and penile erection induced by apomorphine, oxytocin and adrenocorticotropic hormone (acth1-24) was studied in male rats. in sham-operated rats, apomorphine (50 micrograms/kg s.c.), oxytocin (30 ng i.c.v.), and acth1-24 (10 micrograms i.c.v.) significantly increased the number of yawning and penile erection episodes. in pvn-lesioned rats, apomorphine- and oxytocin-, but not acth-induced response ... | 1987 | 2825910 |
| role of the autonomic nervous system, renin-angiotensin system, and arginine vasopressin during the onset and maintenance of acth hypertension in sheep. | the roles of the autonomic nervous system, renin-angiotensin system, and arginine vasopressin (avp) during the onset of acth-induced hypertension were investigated in conscious sheep. autonomic ganglion blockade or combined adrenergic and cholinergic receptor blockade demonstrated that an intact sympathetic nervous system was not essential for the development or maintenance of the hypertension. autonomic blockade augmented the pressor response to acth, indicating that baroreceptor-mediated refle ... | 1987 | 2825944 |
| role of the autonomic nervous system, renin-angiotensin system, and arginine vasopressin during the onset and maintenance of acth hypertension in sheep. | the roles of the autonomic nervous system, renin-angiotensin system, and arginine vasopressin (avp) during the onset of acth-induced hypertension were investigated in conscious sheep. autonomic ganglion blockade or combined adrenergic and cholinergic receptor blockade demonstrated that an intact sympathetic nervous system was not essential for the development or maintenance of the hypertension. autonomic blockade augmented the pressor response to acth, indicating that baroreceptor-mediated refle ... | 1987 | 2825944 |
| reduction of inositol triphosphate in retinal microvessels by glucose and restimulation by myo-inositol. | the formation of inositol phospholipids (ipls) and inositol phosphate esters (ipes) in response to glucose was studied in isolated retinal microvessels from porcine eyes. retinal microvessels incubated from 60 hr with myo-[3h]inositol were sequentially extracted to obtain ipls and ipes. [3h]inositol-labelled ipls were deacylated to produce the corresponding glycero derivatives. both deacylation products and water-soluble ipes were monitored by anion-exchange chromatography. in the presence of hi ... | 1987 | 2828091 |
| activation of the renin angiotensin system in short aortic cross-clamp times. | | 1988 | 2831013 |
| serological investigations for some bacterial and viral pathogens in fallow deer (cervus dama) and wild boar (sus scrofa) of the san rossore preserve, tuscany, italy. | sera of 43 fallow deer (cervus dama) of the san rossore preserve (tuscany, italy) were examined for antibodies against eight pathogens; one proved positive for brucella sp., 21 for listeria monocytogenes, 34 for chlamydia psittaci, three for coxiella burnetii, one for infectious bovine rhinotracheitis virus, 11 for parainfluenza-3 virus, 25 for bovine viral diarrhea virus and six for bovine respiratory syncytial virus. no age and sex difference in the positivity rates and titers was evidenced, w ... | 1988 | 2832622 |
| viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in finland. | during an outbreak of paralytic poliomyelitis in finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater helsinki district and in 13 other cities or towns all over the country. after the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 m ... | 1988 | 2833160 |
| two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance. | insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ... | 1988 | 2834824 |
| two mutant alleles of the insulin receptor gene in a patient with extreme insulin resistance. | insulin receptor complementary dna has been cloned from an insulin-resistant patient with leprechaunism whose receptors exhibited multiple abnormalities in insulin binding. the patient is a compound heterozygote, having inherited two different mutant alleles of the insulin receptor gene. one allele contains a missense mutation encoding the substitution of glutamic acid for lysine at position 460 in the alpha subunit of the receptor. the second allele has a nonsense mutation causing premature cha ... | 1988 | 2834824 |
| epstein-barr virus infection and hepatic fibrin-ring granulomas. | hepatic fibrin-ring granulomas were the main histological finding in the liver of a 38-year-old man with epstein-barr virus primary infection. the patient presented with fever, hepatomegaly, icterus, abnormal liver tests, autoimmune hemolytic anemia, and mononucleosis syndrome. there was neither enanthema nor lymphadenopathy or splenomegaly. serologic tests disclosed an epstein-barr primary infection profile: anti-viral capsid antigen igm antibodies and anti-early antigen antibodies were present ... | 1988 | 2836298 |
| suspected superphosphate poisoning in calves. | | 1987 | 2838010 |
| inhibition of phagocytosis and interleukin-1 production in pulmonary macrophages from rats with sialodacryoadenitis virus infection. | to test whether or not sialodacryoadenitis virus (sdav) infection in rats affects pulmonary macrophage function, we intranasally inoculated pathogen-free f344 rats with sdav and collected alveolar and interstitial macrophages 5 d later. we assessed fc receptor-mediated attachment and phagocytosis by phase-contrast microscopic examination of monolayers of alveolar and interstitial macrophages incubated with zymosan, nonopsonized sheep erythrocytes, or erythrocytes opsonized with rabbit antisheep- ... | 1988 | 2841399 |
| [experimental study on liver cancer--effect of intra-arterial injection of adriamycin-lipiodol emulsion on vx2 liver tumor]. | | 1988 | 2846718 |
| norepinephrine and gtp-gamma-s increase myofilament ca2+ sensitivity in alpha-toxin permeabilized arterial smooth muscle. | a new method for preparing permeabilized smooth muscle fibers from rabbit mesenteric artery has been developed using alpha-toxin, a transmembrane pore-making exo-protein produced by staphylococcus aureus. after alpha-toxin treatment the fibers developed tension as a function of ca2+ concentration (ec50 = 890 nm). but they could not contract without added atp, indicating atp is permeable. when the sarcoplasmic reticulum was loaded with 5 x 10(-7) m ca2+ solution, ne induced a transient contractio ... | 1988 | 2849434 |
| three-dimensional structure of renal na,k-atpase determined from two-dimensional membrane crystals of the p1 form. | electron microscopy and image processing were used to reconstruct a three-dimensional model of membrane-bound monomeric renal na,k-atpase from negatively stained two-dimensional crystals of the p1 type. correlation methods were applied to obtain projection averages which were aligned by a phase difference minimization procedure. the self-consistency of the reconstruction process was high as determined by correlation between experimental projections and projections of the calculated model. the th ... | 1988 | 2850317 |
| characterization of enhanced intestinal permeability; electrophysiological study on the effects of diclofenac and ethylenediaminetetraacetic acid. | the mechanism of the enhancing effects of diclofenac sodium and disodium ethylenediaminetetraacetate (edta) on intestinal permeability to sulphanilic acid were investigated in the rat isolated jejunum by an electrophysiological technique. the results suggested that diclofenac sodium increased the mucosal-to-serosal flux rate of sulphanilic acid by enhancing both transcellular and paracellular permeabilities while the effect of edta was located only in the paracellular pathway. | 1985 | 2863363 |
| kawasaki disease in european adult associated with serological response to coxiella burneti. | | 1985 | 2863526 |
| mothers can prepare and use rice-salt oral rehydration solution in rural bangladesh. | a feasibility study of rice-salt solution for oral rehydration (or) was conducted in a village in the chandpur area of rural bangladesh in 1983. 305 mothers of children aged 0-4 years were interviewed with a pretraining questionnaire to find out about the availability of rice. the mothers were then trained and encouraged to use rice-salt or solution to treat patients with diarrhoea. evaluation of training was assessed by means of field diarrhoea surveillance, case follow-up, rice-salt or solutio ... | 1985 | 2863551 |
| kawasaki disease and coxiella burneti. | | 1985 | 2864572 |
| diagnosis and control of rickettsial diseases. | common isolation procedures on chick embryos and laboratory animals are not of great importance for routine diagnosis of rickettsioses. detection of rickettsiae in skin lesions by immunofluorescence technique allows early diagnosis of rocky mountain spotted fever (rmsf). broad spectrum of methods is at disposal for serological diagnosis of rickettsial diseases. their choice is determined by laboratory equipment, professionality of laboratory staff, economy and simplicity of the given test. thoug ... | 1985 | 2864838 |
| histological changes in mouse liver and spleen caused by different coxiella burnetii antigenic preparations. | the effect was examined on mouse liver and spleen (inbred line a) of intraperitoneal (i.p.) inoculation of phase i coxiella burnetii (c.b.) cells either untreated or treated with chloroform-methanol (cm) mixture in comparison to the trichloracetic acid extract (tcae) from phase i c.b.) cells. the phase i c.b. cells were highly toxic as manifested by marked hepatosplenomegaly accompanied with hyperplastic, degenerative and necrotic changes in the liver. by contrast, phase i cm--treated c.b. cells ... | 1985 | 2866695 |
| q fever pneumonia associated with exposure to wild rabbits. | 4 patients with atypical pneumonia and a history of exposure to wild rabbits were found to have antibodies to coxiella burnetii but not to the other organisms also commonly associated with atypical pneumonia. 10 (45%) of 22 snowshoe hares caught in the area where 1 of the patients snared his rabbits had antibodies to coxiella burnetii. q fever ought to be included in the differential diagnosis of pneumonia acquired following exposure to wild rabbits. | 1986 | 2868348 |