| production of monoclonal antibody against a hemolysin (vp-trh) produced by vibrio parahaemolyticus. | the antigenicity of a hemolysin (vp-trh: vp-tdh related hemolysin) produced by kanagawa phenomenon-negative clinical isolates of vibrio parahaemolyticus was studied using monoclonal antibodies (mabs). a total of 12 hybridoma clones which produced mabs against vp-trh were established. all mabs contained the kappa light chain and were igg type. these mabs were divided into a minimum of 5 different specificity groups, including antibodies specific to vp-trh and common to both vp-trh and vp-tdh, a p ... | 1990 | 2332155 |
| incidence of urea-hydrolyzing vibrio parahaemolyticus in willapa bay, washington. | a high incidence (71.5%) of vibrio parahaemolyticus was found in samples of water, oysters, and sediment from a washington state estuary which produces a significant amount of commercial product. strains of v. parahaemolyticus capable of hydrolyzing urea comprised 58.4% of all v. parahaemolyticus isolates tested. values for fecal coliforms were within certification criteria for commercial harvest and were not correlated with levels of v. parahaemolyticus. | 1990 | 2339878 |
| improved fluorogenic assay for rapid detection of vibrio parahaemolyticus in foods. | an improved fluorogenic assay for the rapid detection of vibrio parahaemolyticus was developed. in the improved assay, the enrichment of v. parahaemolyticus was carried out in arabinose-glucuronate medium (0.5% arabinose, 0.25% glucuronate, 0.1% polypeptone, 0.1% yeast extract, 0.1% ammonium sulfate, 2% nacl, 2 micrograms of polymyxin b sulfate per ml, ph 8.5) at 37 degrees c. after the cultivation, the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substr ... | 1990 | 2339897 |
| fatalities associated with vibrio parahaemolyticus and vibrio cholerae non-o1 infections in florida (1981 to 1988). | vibrio infections constitute a continuing source of morbidity and mortality in florida. seven fatal infections caused by vibrio parahaemolyticus or v cholerae non-o1 were reported in florida between 1981 and 1988. review of those seven medical records and vibrio case investigation forms showed that although all patients died of sepsis, gastrointestinal signs and symptoms characterized the early illness in four patients, whereas the other three initially had painful swelling and/or lesions of the ... | 1990 | 2343327 |
| identification of 2-acetamido-3-o-(3-acetamido-3,6-dideoxy-beta-d-glucopyranosyl)-2-deoxy -d-galactopyranose isolated after degradation of the lipopolysaccharide from vibrio parahaemolyticus serotype o12. | | 1990 | 2346939 |
| bacterial aetiology of infantile diarrhoea in papua new guinea. | bacterial examination of stools of children with diarrhoea was carried out at port moresby, papua new guinea, where little information is available concerning the causative bacteria of diarrhoea. shigellae, enterotoxigenic escherichia coli, bacillus cereus, staphylococcus aureus, campylobacter jejuni, and aeromonas hydrophila were isolated with the decreasing frequency in that order. among these, enterotoxigenic e. coli and c. jejuni were isolated for the first time in this country. although vib ... | 1990 | 2362308 |
| pathogenesis of vibrio parahaemolyticus: intraperitoneal and orogastric challenge experiments in mice. | it has been noted that v. parahaemolyticus isolated from patients with food poisoning are almost always thermostable direct hemolysin (tdh)-positive, whereas, isolates from foods or environmental sources are usually tdh-negative. the virulence of v. parahaemolyticus in mice was examined by using intraperitoneal and orogastric challenge models, where the strains used were those isolated from patients and foods with food poisoning and included tdh-positive and -negative. the ld50 in mice was estim ... | 1990 | 2362562 |
| vibrio bacteremia in siriraj hospital. | from january 1983 to march 1988, 26 isolates of vibrio spp. were recovered from the blood of patients admitted to siriraj hospital. thirteen strains were identified as non 0-1 vibrio cholerae, 3 were vibrio vulnificus and 10 were vibrio spp. the medical records of 20 patients were available for clinical analysis. most of them were adult men with cirrhosis. clinical features included fever, abdominal pain, diarrhea, peritonitis, shock and skin lesions. some patients had a history of seafood consu ... | 1990 | 2380645 |
| [antibacterial and anti-hemolysin activities of tea catechins and their structural relatives]. | among catechins tested, (-)epigallocatechin (egc), (-)epicatechin gallate (ecg), (-) epigallocatechin gallate (egcg) inhibited the growth of staphylococcus aureus, vibrio cholerae o1 classical inaba 569b and el tor inaba v86. s. aureus was more sensitive than v. cholerae o1 to these compounds. egcg showed also a bactericidal activity against v. cholerae o1 569b. pyrogallol showed a stronger antibacterial activity against s. aureus and v. cholerae o1 than tannic and gallic acid. rutin or caffein ... | 1990 | 2381042 |
| [pharmacokinetic, bacteriological and clinical studies in the pediatric field on norfloxacin]. | we have evaluated norfloxacin (nflx), a fluoroquinolone agent, in tablet form for its efficacy and safety in the field of pediatrics. 1. mean serum concentrations of nflx following oral administration to 3 children at dose levels of 3.2 mg/kg, 3.7 mg/kg and 5.4 mg/kg were, respectively, 0.7 microgram/ml, 0.18 microgram/ml and 0.64 microgram/ml at 2-4 hours. mean serum half-lives (t1/2) of nflx were 2.5-2.9 hours and mean urinary recovery rates in the first 6 hours after administrations were 7.1- ... | 1990 | 2391751 |
| sex differences in hormonal responses of vasopressin pathways in the rat brain. | vasopressin (avp) immunoreactivity in cells and projections of the bed nucleus of the stria terminalis (bst) and medial amygdaloid nucleus (ma) depends on gonadal steroids. in addition, the avp projections from the bst show denser fiber staining in males than in females. to study whether these differences depend on different hormone levels in adulthood, male and female rats were gonadectomized and similarly treated with testosterone for 4 weeks prior to sacrifice. immunocytochemistry showed that ... | 1990 | 2394985 |
| incidence of vibrio parahaemolyticus in u.s. coastal waters and oysters. | oyster and seawater samples were collected seasonally from may 1984 through april 1985 from shellfish-growing areas in washington, california, texas, louisiana, alabama, florida, south carolina, virginia, and rhode island which had been designated as approved or prohibited by the national shellfish sanitation program. fecal coliforms counts, aerobic plate counts, and vibrio parahaemolyticus densities were determined for the samples. mean v. parahaemolyticus density was more than 100 times greate ... | 1990 | 2403249 |
| [identification of the causative agent of pasteurellosis]. | | 1985 | 2409344 |
| vertical transmission of enteric pathogens at birth. | to determine how frequently mothers infect their infants with enteric pathogens at birth, stools were collected from 75 thai mothers immediately before delivery and from their infants 24-48 h later. enteropathogenic escherichia coli (epec) were isolated from 25 of the 75 mothers just prior to delivery (32%), enteroviruses from six (8%), enterotoxigenic e. coli from five (7%), and shigella, salmonella and vibrio parahaemolyticus each from one (1%). epec serotype 0128:k67 was isolated from one mot ... | 1985 | 2409892 |
| characterization of an unique rna initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase ii-dependence, tissue specificity, and subcellular location. | we have identified an abundant transcript initiated upstream from the canonical cap site of human alpha 1 globin gene in bone marrow cells and in cos-7 cells transfected with an alpha 1 globin gene-containing plasmid. similar to the major alpha 1 globin transcript, this upstream rna is present almost exclusively in the cytoplasm of the transfected cos-7 cells. it is also synthesized efficiently in vitro by rna polymerase ii in the nuclear extracts prepared from a hela cell line and an erythroleu ... | 1985 | 2413422 |
| protective characteristics of the antigen common to pseudomonas and vibrio and significance of bacterial endotoxins in protection against selected bacterial infections. | the protective effect of different vaccines against the killing activity of pseudomonas aeruginosa and vibrio parahaemolyticus for mice was studied. cross experiments confirmed the high protective effect of the pseudomonas common antigen (original endotoxin protein) contained in pseudomonas vaccine, which, together with other soluble components, substantial afforded protection of mice against vibrios and thus confirmed the antigenic relationship between pseudomonas and vibrio. satisfactory resul ... | 1985 | 2413656 |
| natural killer cytotoxic factor induction by k562 cells in patients with advanced cancer: correlation with production of interferon. | advanced cancer patients were studied for their ability to produce natural killer cytotoxic factor (nkcf). of 23 patients with advanced epithelial cancers, 8 showed deficient natural killer (nk) activity, as measured in a standard 51cr release assay. lymphocytes from these patients did not generate nkcf (nonproducers) in the presence of k562 cells. in addition, 7 other patients whose nk activity was in the normal range did not generate nkcf. thus the deficiency in nkcf production was only partia ... | 1985 | 2415742 |
| serotonin and 5-hydroxyindoleacetic acid in brains of suicide victims. comparison in chronic schizophrenic patients with suicide as cause of death. | serotonin (5-ht) and 5-hydroxyindoleacetic acid concentrations were determined in various brain areas of nonschizophrenic suicide victims, chronic schizophrenic patients with or without suicide as the cause of death, and normal control subjects without psychiatric or neurologic disorders. serotonin concentrations in the basal ganglia were significantly elevated in suicide victims and chronic schizophrenic patients, as were 5-hydroxyindoleacetic acid concentrations in the occipital cortex. these ... | 1986 | 2423050 |
| growth of human mammary carcinoma cells from biopsy specimens in serum-free medium on extracellular matrix. | a method developed for the initiation and maintenance in primary culture of human normal mammary epithelial cells was adopted for the growth of epithelial cells from 45 primary human breast tumors. the cells were grown on a naturally produced extracellular matrix (ecm) or on regular tissue culture plastic in a serum-free medium containing growth supplements and high-density lipoprotein (hdl). successful enzymatic dissociation of the tumor biopsy into organoid structures and cell aggregates was c ... | 1986 | 2427456 |
| the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr. | a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo. | 1987 | 2435577 |
| relationship of myocardial metabolism and coronary flow: dependence on extracellular calcium. | the hypothesis that extracellular [ca2+] ([ca2+]e) influences the transmission of vasoregulatory information from the heart to coronary smooth muscle was examined. the isolated, retrogradely perfused rat heart was used to evaluate vascular reactivity to transient infusion of either 0.88 mm amytal (amobarbital), hypoxia (95% n2-5% co2), or 12 microm adenosine in the presence and absence of 2.5 microm verapamil. amytal alone enhanced coronary flow by 78% and lactate efflux by 10-fold, whereas it d ... | 1987 | 2446512 |
| definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides. | experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ... | 1987 | 2446556 |
| heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells. | cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ... | 1988 | 2446745 |
| bleomycin increases superoxide production in the most active alveolar macrophage subpopulation. | pig alveolar macrophages were separated into three subpopulations by density centrifugation. the most dense subpopulation of macrophages had the greatest rate of superoxide anion production of 4.4 +/- 2.6 nmol/1 x 10(6) cells/min. bleomycin had its greatest effect on this subpopulation by increasing the rate by nearly 100% in cells stimulated with phorbol myristate acetate or phorbol dibutyrate. bleomycin increased the maximum rate of superoxide production without changing the apparent km of 0.0 ... | 1987 | 2447024 |
| chinese hamster ovary cell lysosomes rapidly exchange contents. | we have used cell fusion to address the question of whether macromolecules are rapidly exchanged between lysosomes. donor cell lysosomes were labeled by the long-term internalization of the fluid-phase pinocytic markers, invertase (sucrase), lucifer yellow, fitc-conjugated dextran, or texas red-conjugated dextran. recipient cells contained lysosomes swollen by long-term internalization of dilute sucrose or marked by an overnight fitc-dextran uptake. cells were incubated for 1 or 2 h in marker-fr ... | 1987 | 2447096 |
| stimulation of macrophages by lipopolysaccharide of vibrio parahaemolyticus. | the effect of lipopolysaccharide (lps) from vibrio parahaemolyticus on the biochemical and phagocytic activities of murine peritoneal macrophages was determined. intraperitoneal treatment with different doses (0.5-25 micrograms) of v. parahaemolyticus lps markedly increased the cellular rna content as well as lysosomal enzyme activities of peritoneal macrophages. the treatment also stimulated the phagocytic activities of macrophages. these observations suggest that v. parahaemolyticus lps causes ... | 1987 | 2449599 |
| [catabolite repression of extracellular amylase synthesis in vibrio parahaemolyticus]. | | 1987 | 2452258 |
| monoclonal antibodies to o4 antigen of vibrio parahaemolyticus. | four monoclonal antibodies were prepared against o4 antigen of vibrio parahaemolyticus. all the antibodies were shown to be specific for o4 antigen by agglutination with heat-killed o-cells of the organism and precipitation with lps preparations. the inhibition experiments of the precipitations with various sugars and oligosaccharides suggested that the combining sites of these hybridoma antibodies were directed to an antigenic determinant structure containing----3 and----6 linked d-glucose, d-g ... | 1988 | 2453784 |
| comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus. | hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ... | 1988 | 2466545 |
| [molecular biology of keratins and their significance in the clinical diagnosis of tumors]. | keratins are the most heterogenic class of intermediary filament molecules and form tonofilaments in all types of epithelial cells. at least 19 different human keratins have been demonstrated. on the basis of biochemical and immunological criteria, these may be subdivided into two subtypes (type i and type ii). recent molecular biological investigations have provided considerable insight into the construction of the keratin molecule and the tonofilament structure and the corresponding gene struc ... | 1989 | 2467415 |
| [a new combination of o- and k-antigens of a vibrio parahaemolyticus strain isolated from a patient with diarrhea]. | vibrio parahaemolyticus strain 10260 was isolated from a patient with diarrhea. biochemical and serological characters of the strain were analyzed. this strain possessed a new combination of o- and k-antigens. from the results of this study, the serotype of v. parahaemolyticus strain 10260 was proposed as o1:k20. | 1989 | 2480459 |
| [bacterial etiologies of acute infantile diarrhea in dakar]. | systematic research into all the bacterial etiological agents responsible for acute diarrhoea was undertaken on 276 diarrhoea stools collected from children aged 0-5 years. particular attention was given to finding bacterial agents usually overlooked during routine checks: campylobacter, vibrionaceae and yersinia enterocolitica. | 1989 | 2491376 |
| immunocytochemical study of localization and traffic of thyroid peroxidase/microsomal antigen. | we studied the distribution of binding sites for anti-peroxidase monoclonal antibody and anti-microsomal antibodies on isolated human thyroid follicles and a human thyroid cell line. both open follicles and cells were incubated first with antibodies at +4 degrees c, then with colloidal gold labelled protein a. the topography of the binding sites for monoclonal anti-peroxidase antibody corresponded closely to the expected cell surface distribution of endogenous thyroid peroxidase since labelling ... | 1989 | 2491623 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | | 1989 | 2492809 |
| in vitro development of neospora caninum (protozoa: apicomplexa) from dogs. | the development of neospora caninum isolated from naturally infected dogs was examined in mammalian cell cultures. tachyzoites developed by endodyogeny when inoculated onto bovine monocyte or bovine cardiopulmonary artery endothelial cell cultures. tachyzoites were 5.0 by 2.0 microns and had a posteriorly located nucleus. cytopathogenic effects of parasite development consisted of the formation of holes in the cell monolayer associated with the rupture of infected host cells. serial passage of t ... | 1989 | 2493088 |
| interactive effects of nitrogen and copper on growth of cyanobacterium microcystis. | | 1989 | 2493272 |
| interleukin 2 receptor directed therapy in rat recipients of cardiac allografts: synergistic and antagonistic interactions with the gamma interferon network. | | 1989 | 2495637 |
| the immunodiagnosis of schistosoma mansoni infection in the different clinico-parasitological stages. | a total of 52 bilharzial mansoni patients were examined, 20 patients with early intestinal infection with living ova in stools, 20 patients with hepatosplenomegaly but without ascites, also with viable ova in stools, and 12 patients with hepatosplenomegaly and ascites without ova in stools; but in the rectal snip. also 20 normal persons were examined. an idt test was performed by s. mansoni adult worm antigen. s. mansoni adult worm and egg antigen extracts were used for performing ciep, ihat and ... | 1989 | 2504828 |
| homogeneity of a hemolysin(vh-rtdh) produced by clinical and environmental isolates of vibrio hollisae. | the characteristics of vh-rtdh, a hemolysin similar to vp-tdh of vibrio parahaemolyticus produced by clinical and environmental isolates of vibrio hollisae, were comparatively studied. all 7 strains of v. hollisae tested were found to produce indistinguishable vh-rtdh when they were examined by heat-stability test, western blotting analysis and conventional polyacrylamide slab gel electrophoresis. | 1989 | 2513246 |
| time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated cycles. | in this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ... | 1989 | 2515208 |
| the estimation of dietary protein intake in chronic renal failure. | forty-five adult clinic patients with chronic renal failure each supplied a 4-day weighed dietary record, a 24-h urine collection, and a nocturnal spot urine sample. total nitrogen (n) losses derived from the urines were corrected for proteinuria and non-urea nitrogen excretion. individual estimates of n intake were compared by correlation and assessing the level of agreement. daily urea n excretion derived from the spot sample correlated well with the 24-h collection p less than 0.001, but the ... | 1989 | 2515492 |
| a respiratory-driven and an artificially driven atp synthesis in mutants of vibrio parahaemolyticus lacking h+-translocating atpase. | mutants of vibrio parahaemolyticus lacking the h+-translocating atpase were isolated to evaluate both the role of this enzyme and the possibility of the involvement of other cation-translocating atpase in the energy transduction in this organism. dicyclohexylcarbodiimide-sensitive atpase activity which represents the h+-translocating atpase was not detected either in the membrane vesicles or in the cytosol of the mutants. three major subunits, alpha, beta and gamma, of the h+-translocating atpas ... | 1989 | 2522319 |
| isolation, characterization and immunological determination of basement membrane-associated heparan sulfate proteoglycan. | basement membrane-associated heparan sulfate proteoglycan (hspg) was extracted from isolated porcine glomerular basement membranes and purified by ion-exchange chromatography. the proteogycan was characterized by specific enzymatic digestions, by amino-acid analysis, by sds-polyacrylamide gel electrophoresis and by density gradient centrifugation. polyclonal antibodies were raised against the purified hspg in rabbits. antibodies were characterized by enzyme immunoassays, immunoprecipitation and ... | 1989 | 2533508 |
| use of synthetic ribosome binding site for overproduction of the 5b protein of insertion sequence is5. | insertion sequence is5 is a bacterial transposable element which contains three open reading frames designated 5a, 5b and 5c. although there was no detectable expression from the 5b open reading frame when it was preceded by the native promoter and ribosome binding site or by a tac promoter and the native ribosome binding site, we have overproduced a 5b protein both in vitro and in escherichia coli cells by using a tac promoter and a specially-designed synthetic ribosome binding site. beta-galac ... | 1989 | 2538799 |
| characterization of putrescine production in nongrowing vibrio parahaemolyticus cells in response to external osmolality. | nongrowing vibrio parahaemolyticus cells rapidly produced putrescine (put) from added arginine when subjected to a low osmotic stress. this phenomenon was characterized in connection with a regulatory mechanism of the responsible enzymes, arginine decarboxylase (adc) and agmatine ureohydrolase (auh). nacl, kcl, licl, sucrose, and glycerol were used as solutes to prepare the resuspending media with various osmolalities. regardless of whether the solutes were electrolytes or non-electrolytes, expo ... | 1989 | 2543889 |
| purification and characterization of membrane-bound 5'-nucleotidase of vibrio parahaemolyticus. | membrane-bound 5'-nucleotidase from vibrio parahaemolyticus was solubilized and purified using a nonionic detergent, heptyl-beta-d-thioglucoside, and was characterized. this enzyme required mg2+ for activity, maximum activity being observed at 5 and 20 mm mg2+ with amp and atp, respectively, as substrates. of the divalent cations tested, mn2+ and co2+ were able to replace mg2+ partially, whereas ca2+ was ineffective. zinc strongly inhibited the enzyme activity and ni2+ caused partial inhibition. ... | 1989 | 2546926 |
| cloning and expression of the 5'-nucleotidase gene of vibrio parahaemolyticus in escherichia coli and overproduction of the enzyme. | the gene encoding the membrane-bound 5'-nucleotidase of vibrio parahaemolyticus was cloned and expressed in escherichia coli. cells of e. coli harboring a plasmid, pnut5, which carries the 5'-nucleotidase gene were able to grow on atp as the sole source of carbon, although the original cells were not. the 5'-nucleotidase activity was detected in whole cells of e. coli harboring pnut5 and in membrane vesicles prepared from these cells. most properties of the 5'-nucleotidase produced in e. coli, t ... | 1989 | 2546929 |
| hypoxic changes in hippocampal neurons. | 1. reversible effects of brief periods of anoxia (replacing 95% o2-5% co2 with 95% n2-5% co2 for 2-4 min) were studied in ca1 neurons in hippocampal slices (from sprague-dawley rats), kept in an interface-type chamber at 33.5 degree. 2. the predominant voltage change during anoxia (n2) was a hyperpolarization, accompanied by a marked fall in resistance and excitability; synaptic potentials were also depressed, especially inhibitory postsynaptic potentials (ipsps). 3. in voltage-current (v-i) plo ... | 1989 | 2547034 |
| specificity of transposition of tn7 in vibrio parahaemolyticus. | tn7 was found to transpose at a high frequency from the plasmid, rp4::tn7, to the chromosome of vibrio parahaemolyticus. seven isolates carrying tn7 insertions were derived from three wild-type strains isolated from geographically distinct areas, and hindiii and bsteii dna digests of these strains were probed with a cole1::tn7 biotinylated probe. the results indicated that v. parahaemolyticus is similar to several other species which have been studied in having a highly preferred site of inserti ... | 1989 | 2550986 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| effect of glucocorticoids and limiting nursing on the carbohydrate digestive capacity and growth rate of piglets. | the influence of glucocorticoid administration and limited nursing on piglet carbohydrase enzyme development and subsequent growth was examined in three experiments using 371 piglets. treatments in the first two experiments were formed by the factorial arrangement of hydrocortisone (-hyd or +hyd) and limited nursing (-ln or +ln) imposed form d 14 to weaning (d 28). hydrocortisone was replaced by adrenocorticotropic hormone (acth) in the third experiment. growth rates were severely depressed by h ... | 1989 | 2556355 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| concept of the existence of human papillomavirus (hpv) dna in histologically normal squamous epithelium of the genital tract should be re-evaluated. | because of the crucial importance of guiding current thinking in the field of hpv epidemiology, the concept of the existence of hpv dna in histologically normal squamous epithelium was re-evaluated. a series of 102 randomly collected cervical punch biopsies, previously proved to contain the dna of hpv types 6, 11, 16, 18, 31 or 33 by in situ hybridization were subjected to analysis for the localization of hpv dna, i.e., whether found in the normal epithelium or at the lesion site only. this mate ... | 1989 | 2561041 |
| in-vivo effects of (e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine (mdl 72145) on amine oxidase activities in the rat. selective inhibition of semicarbazide-sensitive amine oxidase in vascular and brown adipose tissues. | one hour after mdl 72145 ((e)-2-(3',4'-dimethoxyphenyl)-3-fluoroallylamine) (2.5 mg kg-1) was given by intraperitoneal injection, the semicarbazide-sensitive amine oxidase (ssao) activity of rat aorta and brown adipose tissue measured in-vitro was reduced by more than 95% of its control value, whereas the monoamine oxidase (mao-a) activity remained virtually unaffected. the action of this drug on amine oxidases in the liver at this dose was less selective. the in-vitro effect of mdl 72145 on the ... | 1989 | 2565962 |
| effects of toxin isolated from the sea anemone bolocera tuediae on electrical properties of isolated rat skeletal muscle and cultured myotubes. | electrophysiological effects of a polypeptide toxin isolated from the sea anemone bolocera tuediae (bttx ii) on isolated fast and slow rat skeletal muscle and on cultured rat myotubes are described in this paper. nanomolar concentrations of bttx ii cause a concentration-dependent depolarization. this effect is prevented by the presence of tetrodotoxin. action potential duration is prolonged in all preparations investigated, with the fast muscle being the least sensitive. the positive overshoot a ... | 1989 | 2566207 |
| genome type analysis of adenoviruses: isolates from one year from the hannover area. | adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ... | 1989 | 2566302 |
| adherence targets of vibrio parahaemolyticus in human small intestines. | formalin-fixed human small intestinal mucosa with mucus coating, villi, and lymphoid follicle epithelium at the mucosal surface was used to test the adherence sites of clinically isolated (kanagawa phenomenon-positive) strains of vibrio parahaemolyticus. v. parahaemolyticus strains grown on cfa agar (supplemented with 3% nacl) for ca. 3 h at 37 degrees c possessed various levels of cell-associated hemagglutinins (has) which were detected with human or guinea pig erythrocytes. the observed adhere ... | 1989 | 2568344 |
| recombinant human erythropoietin treatment in patients on maintenance home haemodialysis. | | 1989 | 2570273 |
| campylobacter pylori, gastrin, acid secretion, and duodenal ulcers. | | 1989 | 2570297 |
| hydrogen peroxide mutagenicity towards salmonella typhimurium. | in bioassays conducted under controlled, comparable conditions, weak direct mutagenicity responses were observed for hydrogen peroxide in the standard (ames test) agar plate incorporation bioassay with salmonella typhimurium strains ta97, ta98, ta102, and ta1537, in a 20 min preincubation test with strains ta97, ta98, ta100, ta102, ta1537, and ta1538, and in a liquid incubation modification using strain ta1537. these results conclusively demonstrate that hydrogen peroxide is a weak mutagen, espe ... | 1989 | 2572065 |
| increased migration inhibition factor production by leukocytes of patients with herpes zoster given the leukocyte ultrafiltrate. | using the direct leukocyte migration inhibition assay, the cell-mediated immune response was followed in together 47 patients suffering from herpes zoster. of these, 19 persons were treated with partially purified and concentrated lysed leukocyte ultrafiltrate. enhancement and/or earlier production of the leukocyte migration inhibition factor--in the presence of varicella-zoster virus antigen--was observed by leukocytes from patients given the ultrafiltrate on days 2-3 since the onset of the ves ... | 1989 | 2576583 |
| the cardiovascular effect of intracerebroventricular endothelin in rats. | to investigate the cardiovascular action of endothelin within the central nervous system, we studied the effect of intracerebroventricular endothelin in conscious wistar rats. the endothelin increased blood pressure and the heart rate in a dose-related way. the increase in mean blood pressure produced by 100 ng/kg of endothelin (45 +/- 6 mmhg, mean +/- s.e.m., n = 10) was much greater than that produced by the same amount of intravenous endothelin (4 +/- 1 mmhg, n = 7). pretreatment with intrave ... | 1989 | 2576664 |
| effect of urapidil on the performance of ischaemic myocardium in anaesthetized dogs. | the effect of urapidil on the ischaemic myocardium was studied in eight anaesthetized dogs. stenosis of the left descending coronary artery reduced blood flow and systolic contraction of the post-stenotic myocardium by about 50%; the end-diastolic length of the post-stenotic myocardium and the end-diastolic pressure increased, while aortic pressure slightly decreased. subsequent administration of urapidil (0.25 + 0.25 + 0.5 + 1.0 mg/kg intravenously) did not affect the systolic shortening and en ... | 1989 | 2576670 |
| [determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method]. | use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ... | 1985 | 2578835 |
| overexpressed pp60c-src can induce focus formation without complete transformation of nih 3t3 cells. | nih 3t3 cells were transfected with plasmids containing moloney murine leukemia virus long terminal repeats and either chicken c-src or v-src genes. in contrast with the effects observed after transfection with plasmids containing c-src and avian retrovirus or simian virus 40 promoter-enhancers (h. hanafusa, h. iba, t. takeya, and f. r. cross, p. 1-8, in g. f. vande woude, a. j. levine, w. c. topp, and j. d. watson, ed., cancer cells, vol. 2, 1984; h. iba, t. takeya, f. r. cross, t. hanafusa, an ... | 1985 | 2582237 |
| [phylogenetic analysis of genes of the influenza virus. relationship between adaptability and neutrality]. | detailed phylogenetic analysis of the gene family of hemagglutinin h3 of influenza a-type virus was fulfilled, taking into account the domain structure of protein and positions of antigen determinants. the densities of distribution of fixed synonimic replacements between domains ha1 and ha2 were shown to be actually equal (rho (ha1) = rho (ha2], and those of nonsynonimic ones to be unequal: their ratios were rho (ha1): rho (ha2) = 2.8 for nonepidemic branches, and rho (ha1): rho (ha2) = 7.7 for ... | 1989 | 2583491 |
| evidence of immunologic cross-reactivity between hemolysins of vibrio hollisae and vibrio parahaemolyticus demonstrated by monoclonal antibodies. | | 1989 | 2584761 |
| comparative amino acid sequence analysis of hemolysins produced by vibrio hollisae and vibrio parahaemolyticus. | vibrio hollisae produces a hemolysin (vh-rtdh) that is related to the thermostable direct hemolysin of vibrio parahaemolyticus (vp-tdh). although both hemolysins are essentially similar biologically and immunologically, they differ markedly in heat stability; vp-tdh is heat stable, whereas vh-rtdh is heat labile. to elucidate the relationships between their characteristics and molecular structures, we analyzed the amino acid sequence of vh-rtdh and compared it with that of vp-tdh. vh-rtdh consis ... | 1989 | 2592352 |
| urease-positive, kanagawa-negative vibrio parahaemolyticus from patients and the environment in the pacific northwest. | we previously reported the occurrence of vibrio parahaemolyticus in patients and the environment in the pacific northwest. the present studies compare the biochemical characteristics, kanagawa hemolysin reactions, and plasmid profiles of 13 patient and 221 environmental isolates of the organism. classical biochemical testing of the isolates revealed similar reactions for the clinical and environmental strains, and analysis in agarose gels revealed that 13 to 15% of the isolates had plasmids. the ... | 1989 | 2592543 |
| emergence of a restricted bioserovar of vibrio parahaemolyticus as the predominant cause of vibrio-associated gastroenteritis on the west coast of the united states and mexico. | examination of 45 human fecal isolates of vibrio parahaemolyticus revealed the emergence of an unusual bioserovar (o4:k12, urease positive) associated with cases of gastroenteritis which appear to be domestically acquired on the west coast of the united states and mexico. | 1989 | 2592553 |
| advances in oculoplastic surgery. | ophthalmic plastic surgery, a sub-specialty within ophthalmology, encompasses diagnosis and treatment of diseases and abnormalities of the eyelids, orbit, and lacrimal systems. eyelid carcinoma is a more common entity as well as blepharoptosis seen in children and adults. it can usually be corrected by surgery involving the levator palpebrae superioris or a tarsofrontalis suspension using synthetic material or human tissue. retraction of the upper eyelid, the opposite of blepharoptosis, may be s ... | 1989 | 2592933 |
| exotic diarrhoeal problems and poliomyelitis. | exotic gastrointestinal infections continue to increase as world travel expands. many are debilitating and some are life-threatening. a heightened awareness of their significance and symptomatology could help to prevent unnecessary suffering or death. | 1989 | 2594659 |
| identification of n1-acetylnorspermidine in vibrio parahaemolyticus and an enzyme activity responsible for its formation. | n1-acetylnorspermidine [ch3conh(ch2)3 nh(ch2)3nh3] was identified in vibrio parahaemolyticus, which contains norspermidine as a major polyamine. this is the first example for the natural occurrence of monoacetylated unusual polyamine. the n1-acetylnorspermidine content was the highest 4 h after inoculation. incubation of norspermidine and acetyl coa with a cell extract from v. parahaemolyticus produced n1-acetylnorspermidine. a remarkable increase in specific activity of the acetyltransferase wa ... | 1989 | 2599358 |
| a mutant hemolysin with lower biological activity produced by a mutant vibrio parahaemolyticus. | a mutant toxin (m-tdh) of thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus was isolated from the culture of a strain of this organism mutagenized with n-methyl-n'-nitro-n-nitrosoguanidine. although the m-tdh had a molecular structure similar to the native vp-tdh, the m-tdh retained only about 7% residual hemolytic activity of the native toxin. furthermore, other biological activities of m-tdh, such as lethality in mice and enterotoxicity in rabbit ileal loops, were also weakened ... | 1989 | 2599363 |
| symptoms associated with diarrhoeal illness at san lazaro hospital, manila in 1983 and 1984. | the clinical features associated with various agents of diarrhoeal disease were studied using 2,836 patients admitted to san lazaro hospital, manila. three general patient groups were considered including single pathogen isolations, "multiple pathogen" isolations, and "no pathogen" isolations. in general, symptoms of diarrhoeal illness were found to be non-specific. however, shigella flexneri. vibrio parahemolyticus, and rotavirus were significantly associated with a number of prominent symptoms ... | 1989 | 2609210 |
| [morphometric and histologic studies of the significance of the paraventricular nucleus for the thyroid gland axis after ganglionectomy and cold exposure with special consideration of the pineal gland]. | to analyse the role of the peripheral sympathetic fibres for the nucleus paraventricularis, the nuclear size and the neurosecretory material were studied 30 d after bilateral cervical ganglionectomy (gx). in order to examine the influence of gx at normal temperature (24 degrees c) and exposure to cold (10 degrees c), rats were subjected to a 72 h exposure to cold before killing. additionally thyroxine (t4), serum thyrotropin (tsh), and pituitary tsh were investigated. 4 times a day (light-dark c ... | 1989 | 2610374 |
| three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout. | the nerve endings of normal hair of the rat's snout, partially digested with trypsin and hydrochloric acid, were studied by scanning electron microscopy. each lanceolate structure measured ca. 10 microns in length and was arranged around the hair follicle. these palisade-shaped nerve endings were situated almost beneath the sebaceous glands, ran upward, parallel to the axis of the hair follicle, and terminated in pointed shape. 2 kinds of cells, teloglia cell type i showing flat profile, and tel ... | 1989 | 2610376 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| effects of postnatal dexamethasone treatment on development of alveoli in adult rats. | we compared the effects of dexamethasone given to rats from age 4 to 14 days, on alveolar development at age 99 days. judged by similar nose-to-tail length and body weight, somatic growth was not altered by dexamethasone treatment. lung volumes in both air- and saline-filled lungs and mean chord length (lm) were increased, whereas alveolar surface area (sa) and surface-to-volume ratio (s/v) were diminished in dexamethasone-treated adult rats. these changes were associated with a significant redu ... | 1989 | 2612450 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| human lung membrane-bound neutral metallo-endopeptidase-catalyzed hydrolysis of bradykinin. | human lung membrane-bound neutral metallo-endopeptidase (nme; ec 3.4.24.11) has been purified; this enzyme occurred in two forms, nme-i and nme-ii. the total nme activity was purified 2,143-fold with the final specific activities for nme-i and nme-ii being 750 and 1,124, respectively. the two nme forms were resolved in the final purification step involving ion exchange; in all earlier steps including gel filtration and affinity chromatography (phenyl sepharose) both forms behaved similarly and e ... | 1989 | 2612455 |
| prp (proline-rich protein) genes linked to markers es-12 (esterase-12), ea-10 (erythrocyte alloantigen), and loci on distal mouse chromosome 6. | the closely linked proline-rich protein (prp) genes, coding for abundant salivary proteins, are located on distal mouse chromosome 6. they are part of a conserved linkage group that is represented on human chromosome 12p. two other markers, ea-10 and es-12, that were previously unassigned to a chromosome are closely linked to prp genes in the mouse. | 1989 | 2613230 |
| gene mapping by enzymatic amplification from flow-sorted chromosomes. | a new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. reliable amplification from as few as 200 chromosomes has been demonstrated. this method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, thy-1, and t3 delta and distal to the int-2 gene. t ... | 1989 | 2613232 |
| production and characterization of a compound inhibitory to vibrio parahaemolyticus from proteus vulgaris. | the production and characteristics of a compound in proteus vulgaris g cultures which was capable of inhibiting vibrio parahaemolyticus and other food-borne pathogens was investigated. production was influenced by medium composition, ph and temperature but not by the extent of aeration. the compound was most inhibitory at the optimum temperature for growth of v. parahaemolyticus. the inhibitor was most stable at ph 7.0 and inhibition occurred even after heating at 70 degrees c for 30 min and aft ... | 1989 | 2613590 |
| in-vitro activity of dr-3355, an optically active isomer of ofloxacin, against bacterial pathogens associated with travellers' diarrhoea. | the in-vitro activity of dr-3355, the s-(-)-isomer of ofloxacin, was determined against bacterial pathogens associated with travellers' diarrhoea. dr-3355 was highly active against isolates of enteropathogenic escherichia coli (mic90 0.05 mg/l), salmonella spp. (mic90 0.10 mg/l), shigella spp. (mic90 0.10 mg/l), campylobacter jejuni (mic90 0.39 mg/l) and vibrio parahaemolyticus (mic90 0.39 mg/l). the activity of dr-3355 against these bacteria was generally two- to eight-fold greater than that of ... | 1989 | 2613604 |
| standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera. | a non-competitive enzyme-linked immunoassay (elisa) has been standardized to supplement the in vivo potency test used for the quality control of inactivated tissue culture vaccines against rabies. the essentials of the elisa were: fixation of the virus in different dilutions of vaccine on the surface of microtitre plates; testing of the reference and up to six test vaccines on one plate; incubation with polyclonal antisera to rabies virus glycoprotein containing an excess of antibody; further in ... | 1989 | 2613705 |
| [the detection of vibrio parahaemolyticus and vibrio alginolyticus using modified culture media]. | studies have been completed for the detection of vibrio(v.) parahaemolyticus and v. alginolyticus in baltic sea fish. the author had prepared for that purpose a liquid culturing medium and a solid substrate. the percentage of positive findings is remarkable. the results so far recorded should be a good reason for further studies. | 1989 | 2619483 |
| haemolysins of vibrio parahaemolyticus strains passaged in mice. | some phenotypically non-haemolytic strains of v. parahaemolyticus were observed to produce haemolysins after two passages in mices. depending on the strain, both heat stable and heat labile haemolysins were induced. the heat stable haemolysin so induced, was immunologically different from the thermostable direct haemolysin produced by kanagawa positive strains of v. parahaemolyticus. | 1989 | 2620943 |
| isolation of the saprophytic strain of mc-3 and participation of the cell surface structure in predation. | from a predatory bacterium, mc-3, a mutant strain which lost predation ability was isolated by chance selection. biological properties of the mutant were the same as the parent except only saprophytic property. properties of the parent and the mutant strains of mc-3, such as bacteriolytic activity of the culture supernatant, digestion of peptidoglycan of the host bacteria, and growth by utilizing the host cells or their cytoplasmic substances, suggested that cell surface structure of the host ce ... | 1989 | 2622396 |
| [study on 438 strains of pathogenic vibrio and their resistance to drugs]. | this paper reported the monitoring result of pathogenic vibrio from 1461 diarrhoea outpatients in haikou city from april to november 1986. 438 strains of pathogenic vibri (29.98%) had been identified. among them the vibrio parahemolyticus (14.99%) was more common and the next was non-0:1 v. cholerae strains (11.84%). the features of attack-time, age, sex and clinical symptoms etc. were discussed. | 1989 | 2624932 |
| effects of food preservatives and local anesthetics on synthesis of outer membrane proteins in vibrio parahaemolyticus. | the effects of seven food preservatives including salicylate, benzoate, dehydroacetate, sorbate and propionate, and two local anesthetics, phenethyl alcohol and procaine, on the synthesis of major outer membrane proteins in vibrio parahaemolyticus strain 3283-61 were investigated. the synthesis of the outer membrane proteins a, b, c and e was remarkably inhibited by all the food preservatives except propionate and two additional outer membrane proteins with respective molecular weights of about ... | 1989 | 2626751 |
| isolation and fundamental properties of a phospholipase a2 inhibitor from the blood plasma of trimeresurus flavoviridis. | phospholipase a2 inhibitor was purified from the blood plasma of habu, trimeresurus flavoviridis, by sephadex g-200 gel filtration, deae-cellulose chromatography, and blue-sepharose cl-6b column chromatography. the purified inhibitor was shown to be a glycoprotein with a molecular weight of about 100k. it was found to consist of four subunits whose molecular weights were around 20-24k. in order to examine the inhibition mechanism of the inhibitor, the interaction of the inhibitor with a phosphol ... | 1989 | 2628432 |
| formation of hydroxynorspermidine from exogenously added 1,3-diaminopropan-2-ol in vibrio species. | when vibrio parahaemolyticus aq 3627 was grown in the presence of 1,3-diaminopropan-2-ol (oh-dap), a new compound accumulated in the cells. this was identified as hydroxynorspermidine (oh-nspd), n-(3-aminopropyl)-1,3-diaminopropan-2-ol, by gas chromatography-mass spectrometry and thin-layer chromatography. it was also synthesized enzymatically from oh-dap by a cell-free preparation from this strain. all other vibrio strains examined also showed the ability to synthesize this compound, but none o ... | 1989 | 2632061 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| studies on medicinal resources from livestock. i. anti-allergic effects of pig bile. (1). | anti-allergic activities of animal biles and commercially available bile acids were evaluated in experimental allergic disease models. pig bile exhibited marked preventive effects on the models of delayed-type hypersensitivity (type iv allergy), picryl chloride-induced contact dermatitis (pc-cd) and sheep red blood cells (srbc)-induced footpad swelling in mice. fel ursi (dried bear gallbladder) also had an inhibitory effect on pc-cd, whereas ox bile, chicken bile and bile acids had no effect on ... | 1989 | 2632089 |
| [the sympathetic and renin-angiotensin systems are complementary in blood pressure regulation]. | the consequences of a chronic destruction of sympathetic nerves or of the blockade of the renin angiotensin system (ras) on the blood pressure (bp) level and its spontaneous variability were studied using a computerized method which allows a continuous monitoring of bp in conscious unrestrained rats. guanethidine, used to lesion the sympathetic fibres did not alter the bp level but significantly enhanced its variability. on the opposite, angiotensin converting enzyme inhibition decreased the bp ... | 1989 | 2633862 |
| short chain fatty acid-induced colitis in mice. | short-chain fatty acids (e.g. acetic, propionic and butyric acid) are metabolic products produced by colonic bacteria. since intra-rectal instillation of dilute acetic acid to rats and cats has been reported to produce a diffuse colitis similar to the human disease, in our study we have investigated the effect of intra-rectal butyric acid in mice. male bka mice (n = 6) received 01 ml of dilute butyric acid by intra-rectal instillation which was flushed out with saline after 10 sec. control anima ... | 1989 | 2634625 |
| in vitro cultures of trabecular meshwork cells of the human eye as a model system for the study of cellular aging. | cells from the human trabecular meshwork providing a drainage system for the outflow of aqueous humour in the eye were isolated and propagated in monolayer culture. following serial subcultivation of the primary cultures, there was a gradual decline in the fraction of dividing cells with increasing population doubling level (pdl) resulting finally in growth cessation and disintegration of these 'senescent' cultures. the number of population doublings was at most 20. senescent cultures revealed r ... | 1989 | 2640083 |
| [effect of two stage corticotomy on maxillary protraction]. | for the purpose of clarifying the effects of two stage corticotomy on maxillary protraction, experimental studies by using of 5 male japanese monkeys (macaca fuscata) were performed. results from cast analysis, roentgenographic cephalometry and histological examinations were as follows. i. in experimental groups that received two stage corticotomy with maxillary protraction, apical base of maxillary bone protruded markedly. ii. in control groups that maxillary protraction was applied without cor ... | 1989 | 2640929 |
| ketopantoyl-lactone reductase from candida parapsilosis: purification and characterization as a conjugated polyketone reductase. | ketopantoyl-lactone reductase (2-dehydropantoyl-lactone reductase, ec 1.1.1.168) was purified and crystallized from cells of candida parapsilosis ifo 0708. the enzyme was found to be homogeneous on ultracentrifugation, high-performance gel-permeation liquid chromatography and sds-polyacrylamide gel electrophoresis. the relative molecular mass of the native and sds-treated enzyme is approximately 40,000. the isoelectric point of the enzyme is 6.3. the enzyme was found to catalyze specifically the ... | 1989 | 2644973 |