| yersinia pseudotuberculosis: report of a case with endoscopic findings. | yersinia pseudotuberculosis is an uncommon gastrointestinal pathogen, recognized as a causative agent in some cases of mesenteric adenitis. we report the clinical and endoscopic features of a case of a 6-year-old girl with abdominal pain and bloody diarrhea. colonoscopic examination revealed aphthous ulcers in the right colon, and mucosal biopsy culture grew yersinia pseudotuberculosis. this case documents right colonic ulcers and bloody diarrhea with yersinia pseudotuberculosis. | 1990 | 2182821 |
| pathways for the penetration of enteroinvasive yersinia into mammalian cells. | for many bacterial species, entry into mammalian cells is an important step toward establishing an infectious disease. genetic and molecular techniques have revealed many important features of the entry process. as an example of this approach, the enteric pathogen yersinia pseudotuberculosis has been used as a model system for bacterial penetration. this analysis has uncovered at least three different pathways for entry of the microorganism into cultured mammalian cells. these pathways differ in ... | 1990 | 2182969 |
| reactive arthritis following yersinia pseudotuberculosis infection. | | 1990 | 2190403 |
| the cytotoxic protein yope of yersinia obstructs the primary host defence. | it has previously been shown that the plasmid-encoded yope protein of yersinia pseudotuberculosis is a virulence determinant. in this study, hela cells, macrophages and mice were used as different model systems to determine the actual role of yope in the virulence process. the yope protein mediates a cytotoxic response on a confluent layer of hela cells. a prerequisite of this activity is that the pathogen binds to the cell surface. yope also induces a cytotoxic response on mouse macrophages whe ... | 1990 | 2191183 |
| genetic evidence that the yopa gene-encoded yersinia outer membrane protein yop1 mediates inhibition of the anti-invasive effect of interferon. | hep-2 cell monolayers were challenged with genetic variants of yersinia pseudotuberculosis ypiii(pib1) and yersinia enterocolitica w22708(pyl4). both strains were represented by (i) variants harboring the 70-kilobase virulence plasmid, (ii) their isogenic plasmid-cured derivatives, and (iii) two transposon mutants constructed by insertional inactivation of the plasmid genes encoding outer membrane protein yop1 and ca2+ dependency in strains ypiii(pib1) and w22708(pyl4). when the hep-2 cells were ... | 1990 | 2194966 |
| [monoclonal antibodies: immunobiotechnologic principles of production and prospects of use]. | | 1990 | 2197169 |
| identification of a seventh operon on plasmid rk2 regulated by the kora gene product. | broad-host-range incp plasmids possess a series of operons involved in plasmid maintenance, whose expression is coordinated by a series of regulators, most of which are encoded in a central regulatory operon. the nucleotide sequence of a new monocistronic operon located between coordinates 55.0 and 56.0 kb on the genome of the incp alpha plasmids rk2 and rp4 is presented. the operon encodes a 34 kda protein which has a net negative charge. transcription of the operon, designated by us kfra (korf ... | 1990 | 2197180 |
| [the basic features of the pathogenesis of pseudotuberculosis (yersiniosis)]. | a new pathogenetic pattern of pseudotuberculosis has been derived on the basis of original clinical, anatomical and experimental findings as well as literature data. careful consideration is given to: a basic mode of infection (alimentary), emergence of the primary affection with a rapid blood invasion by the agent (primary bacteremia), development of specific sensitization, multiorgan secondary focal impairment with lymphogenic dissemination of the infective agent (specific pseudotuberculous po ... | 1990 | 2204325 |
| [clinico-allergic indicators in children with yersinia infection]. | | 1990 | 2204873 |
| yersiniosis. | | 1990 | 2208969 |
| the role of chemosensitive muscle receptors in cardiorespiratory regulation during exercise. | several possible mechanisms leading to the cardiorespiratory adjustments to muscular exercise can be considered. activation of the cardiovascular and respiratory centers may result from: (1) direct or reflex action of circulating metabolites (humoral control); (2) cortical influxes (central drive); (3) nervous impulses from receptors in the contracting muscles (peripheral drive). information presently available focuses most of the interest upon the muscular drive. our studies on anesthetized ani ... | 1990 | 2212481 |
| effect of magnesium lithospermate b on urinary prostaglandins in rats with renal failure. | urinary excretion of prostaglandin e2 showed a significant increase following administration of magnesium lithospermate b [1] in rats with renal failure. in contrast, that of thromboxane b2 showed a significant decrease, indicating the improvement of renal failure. | 1990 | 2213036 |
| homologous dna sequences on the virulence plasmids of pathogenic yersinia and salmonella dublin lane. | yersinia and salmonella harbour plasmids that encode traits important for virulence, enabling both pathogenic genera to survive and grow in cells of the reticulo-endothelial organs during systemic infections. we have detected dna homology between the salmonella dublin virulence plasmid psdl2 and the plasmids of the pathogenic yersinia species pestis, pseudotuberculosis, and enterocolitica. three regions of psdl2 were found to share homology with the virulence plasmid pib1 of yersinia pseudotuber ... | 1990 | 2215215 |
| [transduction of chromosome and plasmid markers of bacteriophage p1 in pseudotuberculosis pathogen]. | a clone of bacteriophage p1 clr100 cml has been isolated capable of the general transduction in the cells of pseudotuberculosis causative agent. the genetic transfer of the 6 md pesticinogenicity plasmid by the bacteriophage has been used as a model to demonstrate the possibility of transduction. the bacteriophage used has been shown to be efficient in interspecies transduction between yersinia. | 1990 | 2215519 |
| yersiniosis in farmed deer. | samples from 77 chital (axis axis), 42 fallow (dama dama), 26 red (cervus elaphus), 7 rusa (cervus timorensis) and 1 sambar deer (cervus unicolor) were examined. yersinia pseudotuberculosis infection was diagnosed as the cause of death in 6 (23%) of the red and 23 (30%) of the chital deer. yersiniosis was the most common infectious cause of death diagnosed. affected deer were usually found moribund or dead, often with faecal staining of the perineum. gross pathology in chital included a fibrinou ... | 1990 | 2222364 |
| modification of the 31p magnetic resonance spectra of a rat tumour using vasodilators and its relationship to hypotension. | the effects of different doses of hydralazine and prostacyclin on the 31p magnetic resonance spectra of the lbds1 fibrosarcoma were investigated and related to their effects on mean arterial blood pressure (mabp) and heart rate. the effect of reducing mabp by bleeding the animals, via the tail artery, was also investigated. tumour spectral changes following high dose drug treatment (an increase in inorganic phosphate, a reduction in nucleotide triphosphates and a reduction in ph) were consistent ... | 1990 | 2223572 |
| modification of the 31p magnetic resonance spectra of a rat tumour using vasodilators and its relationship to hypotension. | the effects of different doses of hydralazine and prostacyclin on the 31p magnetic resonance spectra of the lbds1 fibrosarcoma were investigated and related to their effects on mean arterial blood pressure (mabp) and heart rate. the effect of reducing mabp by bleeding the animals, via the tail artery, was also investigated. tumour spectral changes following high dose drug treatment (an increase in inorganic phosphate, a reduction in nucleotide triphosphates and a reduction in ph) were consistent ... | 1990 | 2223572 |
| actinobacillus rossii sp. nov., actinobacillus seminis sp. nov., nom. rev., pasteurella bettii sp. nov., pasteurella lymphangitidis sp. nov., pasteurella mairi sp. nov., and pasteurella trehalosi sp. nov. | evidence from numerical taxonomic analysis and dna-dna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. the following new species are proposed: actinobacillus rossii sp. nov., from the vaginas of postparturient sows; actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; pasteurella bettii sp. nov., associated with human bartholin gland abscess and finger infections; pasteurella lymphangitidis sp. nov. (the blg group), w ... | 1990 | 2223608 |
| [plasmid dna transformation of yersinia pseudotuberculosis by electroporation]. | rendering cells permeable to dna molecules, electroporation involves the application of high intensity electric fields of short duration to reversibly change the biomembranes, which has been used successfully in some species of both gram negative and positive bacteria. we first reported the electro-transformation technics of yersinia pseudotuberculosis. the plasmid pjgx20 was constructed by cloning the 9.5 kb plasmid encoding pesticin into pst i site of pbr328. transformation efficiency of 103 t ... | 1990 | 2225008 |
| sequence, localization and function of the invasin protein of yersinia enterocolitica. | the inv locus of yersinia enterocolitica is sufficient to convert a non-invasive escherichia coli k12 strain into a microorganism that is able to penetrate cultured mammalian cells. the nucleotide sequence of inv reveals an open reading frame corresponding to an 835-amino-acid protein that is homologous to the invasin protein from yersinia pseudotuberculosis. a polyclonal antiserum elicited by a synthetic peptide corresponding to the c-terminal 88 amino acids of this open reading frame detected ... | 1990 | 2233250 |
| glycerol-blanked triglyceride assays with laboratory animals. | | 1990 | 2242600 |
| [acute renal failure in a generalized form of pseudotuberculosis in children]. | | 1990 | 2251062 |
| morphine attenuation of a conditioned emotional response in post-dependent rats. | rats placed in a test chamber where they had received repetitive shocks the previous day significantly reduced their motor activity; this was taken as indicator of a conditioned emotional response. morphine attenuated this conditioned suppression of motility, possibly due to a reduction of the anxiety associated with the expectation of the noxious stimuli. previous morphine dependence (20 mg/kg daily for 26 days) did not modify the effect of the opioid on the conditioned suppression of motility. ... | 1990 | 2253691 |
| mice and moles inhabiting mountainous areas of shimane peninsula as sources of infection with yersinia pseudotuberculosis. | a total of 1,835 yersinia spp. were isolated from 925 (60.5%) of 1,530 wild mice and from 139 (79.9%) of 174 moles living in mountainous areas of eastern shimane prefecture, japan. the yersinia spp. included 1,106 yersinia enterocolitica, 26 y. enterocolitica-like, 176 yersinia mollaretii, 149 yersinia frederiksenii, 70 yersinia intermedia, 231 yersinia kristensenii, 5 yersinia aldovae, and 72 yersinia pseudotuberculosis. human pathogenic y. enterocolitica was not isolated. y. pseudotuberculosis ... | 1990 | 2254420 |
| [from the parenchyma to the mucosa biopsy: significance and importance of local assays of antibiotics. a critical study]. | the authors discussed the relevance of antibiotic concentrations as measured in tissues and fluids of the respiratory tract. they analysed theoretical factors which influence tissue distribution of antibiotics as well as the results of studies carried out in animal and in human tissues. various factors may interfere with the results of antibiotic assays: but the data published by various groups were usually convergent: this confirms the significance of "tissue pharmacokinetics". new protocols ba ... | 1990 | 2270342 |
| [clinical characteristics of pseudotuberculosis]. | | 1990 | 2274827 |
| [two cases of yersinia pseudotuberculosis infection in children]. | yersinia pseudotuberculosis (y. pseudotuberculosis) infection is an intestinal infectious disease comparable in importance as those with campylobacter or salmonella. clinical symptoms of y. pseudotuberculosis infection vary. in this report, we will describe the clinical symptoms and immunological conditions of the patients with y. pseudotuberculosis infection, including 2 or our own cases. case 1 was a 4 years old male infant admitted to the hospital with major complaints of fever, diarrhea, and ... | 1990 | 2286788 |
| the pyv plasmid of yersinia encodes a lipoprotein, ylpa, related to trat. | a series of lipoproteins was detected in the membrane fraction of yersinia enterocolitica w227, a typical strain from serotype o:9. at least two of them, ylpa and ylpb, are encoded by the pyv plasmid. the sequence of ylpa reveals the presence of a typical lipoprotein signal peptide. the mature ylpa protein would be 223 residues long with a calculated molecular weight of 23798 for the proteic moiety of the molecule. ylpa shares 88% identical residues with the trat protein encoded by plasmid ped20 ... | 1990 | 2287280 |
| [glucocorticoid receptors in physiological states and in extreme conditions]. | this review discusses glucocorticoid hormone--receptor interaction in the animal and human organism in the normal, extreme (stress, radiation, head-down tilt, etc.) and pathological situations. particular attention is given to advanced lines of research into glucocorticoid receptors within the framework of space biology and medicine. it is stressed that the data about glucocorticoid receptors may form the key that can help better understanding of changes induced by exposure to the space environm ... | 1990 | 2292872 |
| a model of multivessel coronary artery disease using conscious, chronically instrumented dogs. | the present investigation was undertaken to develop a reproducible model of multivessel coronary artery disease including a total occlusion of one vessel and a stenosis of an adjacent vessel in chronically instrumented dogs. utilizing sterile techniques, we surgically prepared dogs for measurement of heart rate, arterial pressure, left ventricular pressure, and blood flow velocity in the left anterior descending (lad) and left circumflex (lcca) coronary arteries. a hydraulic occluder and ameroid ... | 1990 | 2292881 |
| transdermal delivery of bioactive peptides: the effect of n-decylmethyl sulfoxide, ph, and inhibitors on enkephalin metabolism and transport. | we investigated the effects of the nonionic surfactant, n-decylmethyl sulfoxide (ndms), ph, and inhibitors on the metabolism and the permeation of amino acids, dipeptides, and the pentapeptide enkephalin, through hairless mouse skin. an hplc gradient method was developed to identify the possible peptide and amino acid metabolites of leucine-enkephalin. ndms increased the permeability of all amino acids and peptides tested. at neural ph, the enzyme activity within the skin was such that no flux o ... | 1990 | 2293206 |
| electron microscopic evidence for in vivo extracellular localization of yersinia pseudotuberculosis harboring the pyv plasmid. | electron microscopic evidence is presented that bacteria harboring the virulence plasmid pyv from yersinia pseudotuberculosis are localized in extracellular sites during the course of infection in mice, often unambiguously undergoing active replication. virulent pyv+ bacteria, often seen adherent to platelets, severely restricted granuloma formation, creating necrotic microabscesses poorly populated with inflammatory cells. this contrasts with granulomas produced by pyv- bacteria, which appear t ... | 1990 | 2307522 |
| genetic analysis of the yope region of yersinia spp.: identification of a novel conserved locus, yera, regulating yope expression. | the yope gene of yersinia pseudotuberculosis was recently sequenced, and yope was identified as an indispensable virulence determinant when tested in a mouse model (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). in the study described here, the dna sequences of the yope genes of yersinia pestis ev76 and yersinia enterocolitica 8081 were determined and compared with that of the y. pseudotuberculosis gene. only two codons were found to differ, both leading to amino acid replacemen ... | 1990 | 2307658 |
| multiple beta 1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells. | mammalian cell receptors that promote entry of intracellular bacteria into nonphagocytic cells have not been identified. we show here that multiple members of the integrin superfamily of cell adhesion receptors bind the y. pseudotuberculosis invasin protein prior to bacterial penetration into mammalian cells. affinity chromatography of crude detergent extracts demonstrated that integrins containing the subunit structures alpha 3 beta 1, alpha 5 beta 1, and alpha 6 beta 1 bound to immobilized inv ... | 1990 | 2311122 |
| synchronous fluorescence spectroscopic, immunoaffinity chromatographic and 32p-postlabeling analysis of human placental dna known to contain benzo[a]pyrene diol epoxide adducts. | human placenta readily catalyzes the biotransformation of polycyclic aromatic hydrocarbons (pahs) and other carcinogens to reactive metabolites that can damage dna through formation of covalent adducts. placenta is widely available for epidemiologic studies and may be a useful dosimeter for carcinogen exposures in humans. however, previous studies of human placental dna have yielded discrepant results with respect to pah-dna adducts. in order to resolve some of the issues surrounding these discr ... | 1990 | 2322996 |
| rat toxicity studies with beta-sitosterol. | chronic administration of beta-sitosterol subcutaneously to rats for 60 days was well tolerated and there was no clear cut evidence of any gross or microscopic lesions either in the liver or kidney. liver and kidney function tests were assessed by determining the blood/serum parameters like haemoglobin, blood glucose, serum protein, serum bilirubin, serum cholesterol, serum gpt and serum got. all the parameters were in the normal range except serum protein and serum cholesterol. serum cholestero ... | 1990 | 2329812 |
| tibenelast, 5,6-diethoxybenzo(b)thiophene-2-carboxylic acid, sodium salt (ly186655), is an orally active anti-asthma compound in the guinea pig. | anaphylactic shock was induced in actively sensitized guinea pigs by free inhalation of a high dose of ovalbumin (10 mg/ml) aerosol. tibenelast (ly186655), 5,6-diethoxybenzo(b)-thiophene-2-carboxylic acid, sodium salt, proved to be a potent orally active compound against anaphylactic shock induced by high dose antigen aerosol. when a lower aerosol challenge (0.05 mg/ml) was employed, bronchoconstriction was observed with a concomitant increase in lung resistance (rl) and a fall in dynamic compli ... | 1990 | 2329918 |
| a highly efficient electroporation system for transformation of yersinia. | the various pathogenic yersinia species are not readily and efficiently transformed by classical methods. for this reason, the electroporation technique was applied for genetic transformation of these species. using optimal conditions, we were able to transform the six yersinia strains studied with the two most widely used groups of plasmids: psu2718 (a pacyc184 derivative) and pk19 (a puc19 derivative). only yersinia enterocolitica (y. e.) serotype 0:8 gave poor results (less than 5 x 10(2) tra ... | 1990 | 2332165 |
| [plasmids of yersinia pseudotuberculosis]. | plasmids with the sizes of 5.7; 51; 70-77; and 120-130 kb were found in six strains among the ten strains collection of yersinia pseudotuberculosis. the restriction endonucleases analysis. southern-blot hybridization and physical maps construction were performed for the plasmids. the 70-77 kb plasmids were found to be analogous to the ca2(+)-dependence plasmid pyvo19 from yersinia pestis ev76. the difference between the plasmids of this type is in the insertions or deletions located on the simil ... | 1990 | 2333080 |
| [conjugative r-plasmid resistance of the causative agents of yersinia infection]. | nature, structure, occurrence and drug resistance of 160 strains of y. pseudotuberculosis and 60 strains of y. enterocolitica isolated from various sources within 1986-1988 were studied. in the strains of y. pseudotuberculosis, the cell composition with respect to the requirements in calcium ions as well as the plasmid profiles with determination of the molecular weights of the plasmids in the antibiotic sensitive and resistant pathogens and r(+)-transconjugants were investigated. some molecular ... | 1990 | 2337369 |
| [hepato-renal polycystic disease and liver hydatidosis (a rare association)]. | a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ... | 1990 | 2346686 |
| relationship between dam's age at bull's birth and bull's genetic evaluation. | after accounting for year of birth of bulls, younger dams produced superior sons. bulls' pd, parent average, and pedigree index milk decreased with increasing age of dams at bull's birth. this appears to be due to decrease in maternal grandsire's pd milk and dam's cow index milk with increase in age of dam. younger dams have an advantage of potentially higher genetic merit due to genetic trend but have less information included in their evaluations. bull's latest parent average and pedigree inde ... | 1990 | 2365889 |
| [incidence of tuberculosis in the hunting tribe e-lun-chun in northeast china]. | the e-lun-chun, or ortochen, a national minority in north east china, consisted of 2,262 members in 1953. in the mid-17th century a russian invasion had displaced them from their original home in southward direction over the amur river towards the sin an lin mountain range. while coming into closer contact with neighbouring peoples, tuberculosis and other infectious diseases spread rapidly among the members of the tribe. under the japanese occupation (1932-1945) the incidence of pulmonary tuberc ... | 1990 | 2367435 |
| ciprofloxacin penetration into human breast milk: a case report. | the concentration of ciprofloxacin achieved in human breast milk following oral administration of a single 500-mg dose was investigated. a 24-year-old white woman, 17 days postpartum, was being treated for resolving acute renal failure. oral ciprofloxacin was prescribed for a suspected urinary tract infection. due to suspected pediatric toxicity and a lack of published information describing ciprofloxacin excretion in breast milk, the antibiotic was discontinued after one 500-mg dose. all breast ... | 1990 | 2375140 |
| tertiary structure of oxidized flavodoxin from an eukaryotic red alga chondrus crispus at 2.35-a resolution. localization of charged residues and implication for interaction with electron transfer partners. | the crystal structure of the oxidized form of a flavodoxin from an eukaryotic red alga, chondrus crispus, has been determined by multiple isomorphous replacement and anomalous scattering methods. a model of the 173 residues and flavin mononucleotide (fmn) has been refined by a restrained least squares method to a crystallographic r-factor of 22.6% using 6236 reflections between 6.0 and 2.35 a with f greater than 3 sigma f. this molecule has a sheet consisting of five parallel beta-strands with t ... | 1990 | 2394748 |
| effects of castration and testosterone treatment on the activity of testosterone-metabolizing enzymes in the brain of male and female zebra finches. | recently, we described the distribution of testosterone-metabolizing enzymes (i.e., aromatase, 5 alpha- and 5 beta-reductases) in the zebra finch (taeniopygia guttata) brain using a sensitive radioenzyme assay combined to the palkovits punch method. a number of sex-differences in the activity of these enzymes were observed especially in nuclei of the song-control system. the hormonal controls of these differences have now been analyzed by gonadectomizing birds of both sexes and by giving them a ... | 1990 | 2394994 |
| [activity of antibacterial preparations against the causative agent of pseudotuberculosis in vitro]. | antibacterial activity of 32 chemotherapeutics against 15 strains of yersinia ++pseudotuberculosis was studied in vitro. the majority of the strains or 80% were sensitive to penicillins, cephalosporins, monobactams, aminoglycosides, tetracyclins , anzamycins, fluorine derivatives of quinolonecarboxylic acid, levomycetin and a combination of trimethoprim and ++sulfamethoxazole. it was shown that with respect to the y. pseudotuberculosis strains with multiple antibiotic resistance, the fluorine de ... | 1990 | 2400288 |
| a heat-modifiable outer membrane protein carries an antigen specific for the species yersinia enterocolitica and yersinia pseudotuberculosis. | the outer membranes of gram-negative bacteria are considered to be of importance in host-bacteria interaction, in protective immunity, and occasionally in subclassification within a species. in this study, the outer membranes of several strains of yersinia enterocolitica and y. pseudotuberculosis were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). it was found that the appearance of the major proteins depended on the temperature at which they were solubilized i ... | 1985 | 2409152 |
| [different forms of a lipopolysaccharide-protein complex from yersinia pseudotuberculosis]. | two forms of lipopolysaccharide-protein complex with buoyant densities of 1,43 and 1,40 g/cm3 were found in the yersinia pseudotuberculosis cell wall. these forms have the similar monosaccharide, fatty acid and polypeptide compositions, but differ in the length of o-specific chains. the differences in density are stipulated by the different contents of the main components of the complex. both forms contain the related antigenic determinants but have some differences in the antigenic structure. t ... | 1985 | 2415136 |
| down-regulation of dopamine d-2, 5-ht2 receptors and beta-adrenoceptors in rat brain after prolonged treatment with a new potential antidepressant, lu 19-005. | lu 19-005 is a new phenylindan derivative with strong and equipotent inhibitory effect on dopamine (da), noradrenaline (na) and serotonin (5-ht) uptake. the adaptive effects of 2 weeks treatment with lu 19-005, on receptor binding in vitro and on d-amphetamine responsiveness in vivo have been investigated in rats. one or 3 days after the final dose the number of beta-adrenoceptors and of 5-ht2 and da d-2 receptors was decreased by 20-30%, whereas alpha 1-adrenoceptor number was slightly decrease ... | 1985 | 2418155 |
| characterization of receptors regulating secretory function in the fundic mucosa. | a model for a present view of the major pathways and receptors mediating function in the canine fundic mucosa is depicted in figure 1. gastrin has direct actions on the parietal cell and on the somatostatin cell; action on the parietal cell, but not somatostatin cell, is potentiated by histamine. in contrast, gastrin action on the somatostatin cell is potentiated by beta-adrenergic agonists. the potency of h2 blockers against gastrin may reflect blockage by these inhibitors of the stimulatory (p ... | 1986 | 2423027 |
| vagal primary afferent terminals in the dorsal motor nucleus of the rat: are they making monosynaptic contacts on preganglionic efferent neurons? | after horseradish peroxidase (hrp) application to the cut cervical vagus nerve in rats, labelled primary afferent terminals could be demonstrated in the dorsal motor nucleus at the ultrastructural level by a combined glucose oxidase-silver-gold intensification technique. some labelled boutons contacted labelled dendrites of preganglionic neurons. thus, the occurrence of a few monosynaptic primary afferent-preganglionic efferent contacts in the dorsal motor nucleus could be demonstrated. | 1986 | 2429236 |
| [effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties]. | thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ... | 1986 | 2430684 |
| the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr. | a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo. | 1987 | 2435577 |
| [selection of antigens for constructing immunoenzyme test systems for pseudotuberculosis]. | | 1987 | 2441126 |
| discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha. | the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ... | 1987 | 2441999 |
| topological mapping of complement component c9 by recombinant dna techniques suggests a novel mechanism for its insertion into target membranes. | cdna molecules coding for mouse and trout c9 have been isolated and the derived amino acid sequences compared with that of human c9. regions of high homology between the closely related species (mouse and human) correlate with putative domains in the protein structure supporting a model of c9 having five globular domains. comparison between the more distant species (trout and human) suggests regions of particular importance to c9 structure and function. in addition the three related sequences al ... | 1987 | 2443347 |
| a yersinia pseudotuberculosis protein which cross-reacts with hla-b27. | the most-debated question in the investigation of the spondyloarthropathies has been whether there is molecular mimicry between host hla-b27 antigens and the arthritis-causing pathogens. we have generated a monoclonal anti-hla-b27 antibody in our laboratory and have used a radioimmunoassay to screen a panel of bacterial species. two strains of yersinia pseudotuberculosis were found to be highly reactive. the cross-reactive yersinia component was identified by western blot to be a 19,000 componen ... | 1987 | 2444647 |
| a new look at uterine muscle contraction. | recent progress in our understanding of uterine smooth muscle contraction is reviewed. we no longer believe that actin-myosin interaction in the myometrium occurs through activation of the thin filament; but it is triggered by calcium-dependent phosphorylation of myosin in the thick filament. calcium is now thought to originate from both extracellular and intracellular sources. calcium can enter the cell through either a voltage- or a hormone-controlled calcium channel. the intracellular source ... | 1987 | 2446500 |
| immunosuppressive effects of isoprinosine in man: a comparison to chlorambucil effects in multiple sclerosis. | immunological and clinical functions were studied over a 2 year period in conjunction with a placebo controlled trial of isoprinosine and chlorambucil in 21 patients with exacerbating remitting multiple sclerosis. laboratory and clinical evaluations were performed at 3 month intervals and during relapses. in placebo-treated patients, the decrease in circulating t8+ cells was maximum during relapses, t lymphocyte function was impaired, and five of the six patients experienced clinical worsening. ... | 1987 | 2446758 |
| galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons. | galanin immunoreactivity was observed in nerve cell bodies and nerve fibres, but not in enteroendocrine cells, in the small intestine of the guinea-pig. nerve terminals were found in the myenteric plexus, in the circular muscle, in submucous ganglia, around submucous arterioles, and in the mucosa. lesion studies showed that all terminals were intrinsic to the intestine; those in myenteric ganglia arose from cell bodies in more orally placed ganglia. myenteric nerve cells were also the source of ... | 1987 | 2446770 |
| isolation of simian immunodeficiency virus from african green monkeys and seroepidemiologic survey of the virus in various non-human primates. | sixteen isolates of simian retrovirus closely related to human immunodeficiency virus (hiv) were obtained from healthy african green monkeys (agm) (cercopithecus aethiops). the first isolate was obtained from a monkey seropositive for hiv, and the others were isolated from monkeys harboring antibodies to the first isolate. these simian retroviruses were referred to as simian immunodeficiency virus from agm, siv[agm], due to their cross-reactivities with hiv structural proteins. these siv[agm] is ... | 1988 | 2447023 |
| changes of chromatin organization induced by phospholipids. | isolated nuclei represent a suitable model for studying the influence of exogenous phospholipids, normally found as minor chromatin components, on the nuclear structure, which, in turn, could be related to the observed modifications of dna and rna synthesis. the morphological modifications induced on chromatin rnp granules and nuclear matrix have been analyzed both with conventional thin sectioning and with an original method based on image analysis of freeze-fractured and replicated nuclear sam ... | 1987 | 2447872 |
| cloning and characterization of the methyl coenzyme m reductase genes from methanobacterium thermoautotrophicum. | the genes coding for methyl coenzyme m reductase were cloned from a genomic library of methanobacterium thermoautotrophicum marburg into escherichia coli by using plasmid expression vectors. when introduced into e. coli, the reductase genes were expressed, yielding polypeptides identical in size to the three known subunits of the isolated enzyme, alpha, beta, and gamma. the polypeptides also reacted with the antibodies raised against the respective enzyme subunits. in m. thermoautotrophicum, the ... | 1988 | 2448287 |
| [synthesis of lipid a analogs. preparation of serologically active glycolipids on the basis of beta-1,4-glucosaminobiose (chitobiose)]. | synthesis of beta-1,4-glucosaminobiose (chitobiose) 4'-phosphates n,n'-diacylated with (r)-3-hydroxymyristic acid is described, the structure being corroborated by 13c-nmr spectra. it was shown that activity of the components in various reactions with antibodies to lipid a from yersinia pseudotuberculosis is similar to the lipid a activity. | 1987 | 2449221 |
| activation of the mas oncogene during transfection of monoblastic cell line dna. | using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene. | 1988 | 2453759 |
| [the use of 13c-nmr spectra of lipopolysaccharide-protein complexes to determine the structure of o-specific polysaccharides]. | lipopolysaccharide-protein complexes are shown to be useful for elucidation of o-specific polysaccharide structures. this approach may be especially useful in studying polysaccharides with labile linkages in the molecule. | 1988 | 2454633 |
| frequency analysis of simian virus 40-specific cytotoxic t lymphocyte precursors in the high responder c57bl/6 mouse strain. | studies in this laboratory have shown that long term simian virus 40 (sv40)-specific cytolytic t lymphocyte (ctl) cultures established from the spleens of high responder c57bl/6 (b6; h-2b) mice exhibit a preference for the selection of h-2db-restricted ctl clones. in this study, we have investigated the basis for this selection. limiting dilution cultures were established using responder cells from the popliteal lymph nodes and the spleens of b6 mice immunized subcutaneously in the hind footpads ... | 1988 | 2459302 |
| [preparation from soviet materials of an immunoenzyme conjugate for detecting pseudotuberculosis]. | | 1988 | 2462119 |
| [differential isolation of low-molecular plasmids]. | several methods for the isolation of plasmid dna from yersinia pseudotuberculosis have been tried; the aim of the study has been a differentiated isolation of low-molecular plasmids from natural strains of bacteria containing plasmids with various molecular masses. the modifications of the known methods have been developed, that rule out the use of lysozyme for cell lysis and permit prepare clarified lysates containing mostly low-molecular plasmid dna from natural strains of bacteria containing ... | 1989 | 2470974 |
| [the structure of the o-specific polysaccharide chain of the lipopolysaccharide of yersinia pseudotuberculosis serovar vii]. | an o-specific polysaccharide of yersinia pseudotuberculosis serovar vii has been isolated and characterized. the polysaccharide consists of colitose, d-glucose and 2-acetamido-2-deoxy-d-galactose in the ratio 1 : 2 : 2. from the results of methylation analysis, partial acid hydrolysis, 1h and 13c nmr spectroscopy the structure of the repeating unit of the o-specific polysaccharide is deduced as follows: | 1989 | 2472792 |
| relationships between ventricular arrhythmias and plasma concentrations of me3202 (cm7857) in dogs. | two-stage coronary ligation-, digitalis-, and epinephrine (epi)-induced ventricular arrhythmias were used to establish minimum effective plasma concentrations of me3202 in dogs. 2-(2-diisopropylaminoethyl)-4-methyl-2-(2-pyridyl) pentanamide (me3202) showed antiarrhythmic effects against arrhythmias in all models. the minimum effective plasma concentrations for arrhythmias induced by 24-h coronary ligation, 48-h coronary ligation, digitalis, and epi were 4.8 +/- 1.0 [after 5 mg/kg intravenously ( ... | 1989 | 2475703 |
| [quantitative determination of determinant groups in specific polysaccharides from yersinia pseudotuberculosis (serostrain i b)]. | | 1985 | 2477671 |
| the bacterial outer membrane protein that reacts with anti-hla-b27 antibodies is the ompa protein. | a bacterial outer membrane protein of 35-kda mr has been reported to react with several anti-hla-b27 mab. here, we demonstrated that this protein showed the heat-modifiability of the ompa protein during sds-page. further, the protein was not detected in mutants of escherichia coli in which the expression of the ompa protein has been suppressed. the protein would be reexpressed when one of the mutants was transformed with an expression vector carrying the ompa gene. finally, the identity of the r ... | 1989 | 2478630 |
| [immunoelectrophoretic analysis of the antigenic composition of yersinia]. | the antigenic composition of 24. y. pseudotuberculosis newly isolated and reference strains, 7 y. enterocolitica strains, as well as y. pestis vaccine strain ev, has been studied by the method of immunoelectrophoresis in agar. the antigenic composition of these bacteria has been found to be complicated and to comprise not less than 8-11 antigens, and among them nonspecific protein antigens common for enterobacteria, the common generic antigen, the antigen common with y. pestis, as well as o-anti ... | 1989 | 2480038 |
| [stationary and migratory avifauna as reservoirs of salmonella, yersinia and campylobacter]. | domestic and wild animals have been always considered very important as reservoir of agents of human infections. particularly birds, because of their great mobility from a continent to another or within the limits of the same ecosystem may transfer pathogenic micro-organisms. the present survey was undertaken in order to evaluate the presence of campylobacter, yersinia and salmonella in migratory and permanent birds. during the period october 1986 to march 1988 intestinal loops were collected fr ... | 1989 | 2483644 |
| [cervical adenitis caused by yersinia pseudotuberculosis]. | | 1989 | 2490440 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | | 1989 | 2492809 |
| endothelial cells from human umbilical vein secrete functional transcobalamin ii. | transcobalamin ii (tcii) is a cobalamin (cbl) binding protein in the plasma that mediates the cellular uptake of cbl. although the synthesis of tcii by a variety of cultured mammalian cells and by some isolated perfused organs has been reported, no single tissue has been identified as the source of tcii in vivo. in this study, we demonstrate that cultured human umbilical vein endothelial cells secrete a protein that binds cn[57co]cbl, elutes from a sephacryl s-200 column in the same position as ... | 1989 | 2493194 |
| monitoring of intestinal transplant rejection. | our results show that maltase, sucrase, and lactase activity are present at a normal level in nonrejecting small bowel transplants after an initial postoperative decline. this confirms that the disaccharide absorbing capacity of these grafts is intact. in allogeneic bowel, however, the levels of maltase and sucrase decline as histologic rejection occurs. these results suggest that serial maltase, sucrase, and possibly lactase levels in allogeneic intestinal transplants may serve as a useful adju ... | 1989 | 2493205 |
| [population dynamics of yersinia pseudotuberculosis in association with the infusorian tetrahymena pyriformis]. | the decisive role of biotic factors in the preservation of the viability of y. pseudotuberculosis is shown. the results of experiments suggest that protozoa may serve as reservoir in the spread of y. pseudotuberculosis infection. | 1989 | 2496540 |
| [the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections]. | between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ... | 1989 | 2503908 |
| leukocyte and plasma n-laurylsphingosine deacylase (ceramidase) in farber disease. | severe deficiency of acid ceramidase activity (4-5% of normal) was demonstrated in cultured skin fibroblasts, leukocytes and plasma from a 1-year-old boy who was diagnosed as being affected with farber disease. determination of ceramidase activity in plasma was achieved by a highly sensitive assay employing a ceramide substrate containing radiolabeled c12 n-acyl moiety (n-lauryl). the enzyme activity in the parents' leukocytes and plasma was found to be reduced to 18-47% of the respective normal ... | 1989 | 2504515 |
| 5'-n-ethylcarboxamide[3h]adenosine binding sites of mouse mastocytoma p815 cell membranes: characterization and solubilization. | mouse mastocytoma p815 cell membranes were found to possess adenosine binding sites as assessed by using the adenosine agonist [3h]5'-n-ethylcarboxamideadenosine (neca). the kd and bmax for the [3h]neca binding at 0 degrees c were 380 nm and 17 pmol/mg of protein, respectively. the rank order of potency for inhibition of [3h]neca binding was neca greater than 5'-n-cyclopropylcarboxamideadenosine greater than 2-chloroadenosine greater than 2',5'-dideoxyadenosine greater than isobutylmethylxanthin ... | 1989 | 2504705 |
| 5'-n-ethylcarboxamide[3h]adenosine binding sites of mouse mastocytoma p815 cell membranes: characterization and solubilization. | mouse mastocytoma p815 cell membranes were found to possess adenosine binding sites as assessed by using the adenosine agonist [3h]5'-n-ethylcarboxamideadenosine (neca). the kd and bmax for the [3h]neca binding at 0 degrees c were 380 nm and 17 pmol/mg of protein, respectively. the rank order of potency for inhibition of [3h]neca binding was neca greater than 5'-n-cyclopropylcarboxamideadenosine greater than 2-chloroadenosine greater than 2',5'-dideoxyadenosine greater than isobutylmethylxanthin ... | 1989 | 2504705 |
| clinical manifestations of yersinia pseudotuberculosis infection in children. | the clinical manifestations of infection were analysed during an outbreak of 34 cases of yersinia pseudotuberculosis serotype ia infection. the diagnosis was based on the results of enzyme immunoassay and verified by stool culture in five cases. the first four patients were pupils from the same school, and information on any signs and symptoms of infection was obtained by questionnaire from pupils of the school. a blood sample was obtained from 101 children. strong igm and igg antibody responses ... | 1989 | 2506017 |
| time of insemination and its effect on in-vitro fertilization, cleavage and pregnancy rates in gnrh agonist/hmg-stimulated cycles. | in this prospective study, we compared the effect of delayed inseminations on fertilization, cleavage and pregnancy rates in two groups of patients. in one group, the ovarian stimulation was performed with a clomiphene citrate/human menopausal gonadotrophin/human chorionic gonadotrophin (cc/hmg/hcg) protocol. the other group was pre-treated with gonadotrophin-releasing hormone agonist (gnrha) and ovarian stimulation was carried out with an hmg/hcg protocol. under both protocols, a delay of 2, 4 ... | 1989 | 2515208 |
| transient, steady-state and rebreathing responses to carbon dioxide in man, at rest and during light exercise. | 1. the transient ventilatory response to co2, measured using short pulses at constant inflow, was compared with the steady-state response at rest and during exercise at 50 w, and with the rebreathing response at rest, in nine healthy subjects. at rest co2 was given at flow rates of 0.2 and 0.4 l min-1 and during exercise, to compensate for the smaller inhaled co2 fraction as ventilation increased, at flow rates of 0.4 and 0.8 l min-1. 2. we calculated two indexes of gain for the transient respon ... | 1989 | 2515277 |
| von recklinghausen's disease of nerves: a modern day social response. | we describe a patient with von recklinghausen's disease of nerves whose appearance provoked an emotive reaction in the close community of an rn ship akin to that reported in the case of sir frederick treves' "elephant man". the rn divisional system provided a valuable contribution to the resolution of the problems created. | 1989 | 2515278 |
| functional, histologic and ultrastructural characteristics of the portal hypertensive gastric mucosa. | | 1989 | 2519020 |
| seasonal patterns of rodents, fleas and plague status in the western usambara mountains, tanzania. | field and commensal rodents were live-trapped at three villages in an active focus of plague (yersinia pseudotuberculosis pestis) in lushoto district, western usambara mountains, tanga region, tanzania, from december 1983 to november 1984. their flea ectoparasites were collected, identified and counted. the rodent carcasses were serologically examined for specific plague antibodies and antigens, and bacteriologically examined for bipolar staining bacilli. a total of 1758 traps were set during th ... | 1989 | 2519642 |
| cloning and characterization of a pectate lyase gene from erwinia carotovora ec153. | a pel gene cloned from strain ec153 of erwinia carotovora encoded a pectate lyase that macerated plant tissue with moderate efficiency. this gene, called pel153, was sequenced and found to possess considerable homology with a pectate lyase gene from yersinia pseudotuberculosis. the yersinia protein, however, was truncated at the carboxyl terminal end relative to the erwinia gene product and had a lower isoelectric point. the erwinia pel153 gene was overexpressed in cells of escherichia coli, and ... | 1989 | 2520159 |
| presence of yersinia specific antibodies in hospitalized patients with arthritis and reiter syndrome. | a number of 50 patients admitted with arthritis and reiter syndrome were investigated to detect the presence of yersinia specific antibodies. the specific antibodies were evidenced in 5 patients (10%)--3 with antibodies to y. enterocolitica and 2 with antibodies to y. pseudotuberculosis, in titers greater than or equal to 1/160. the patients were followed in dynamics up to 3-4 months. the antibodies to y. enterocolitica persisted in time longer than those to y. pseudotuberculosis. for a better k ... | 1989 | 2520672 |
| the role of thromboxane a2 in experimental and human models of chronic glomerular damage. | | 1989 | 2526467 |
| characterization of three functional sites in alpha beta 1 dr of drw13. all three sites are potentially involved in major histocompatibility complex-peptide interaction. | an hla-dr product encoded by the hla-drw13/dw19 haplotype has been identified as the hla class ii molecule involved in antigen presentation to several influenza-specific helper t cell clones. three different functional sites were identified on this molecule by comparing the structure of hla-dr products of known sequences and their ability to efficiently present foreign antigen to the t cell clones. these functional sites were mapped on the recently proposed three-dimensional structure of hla cla ... | 1989 | 2529124 |
| ultrastructure of human granulosa cells obtained from follicular fluid aspirates. | samples of the granulosa cells obtained from a punctured human preovulatory follicles stimulated for in vitro fertilization purposes were studied by means of light and electron microscopic methods. early and late preluteinized granulosa cells were characterized by the presence of high amount of pale and homogeneous lipid droplets, moderate accumulation of glycogene and progressive development of smooth endoplasmic reticulum. a new type of granulosa cell containing dense-cored granules with fine ... | 1989 | 2533848 |
| during latency, herpes simplex virus type 1 dna is associated with nucleosomes in a chromatin structure. | latent herpes simplex virus type 1 dna has a nucleosomal structure similar to that of cellular chromatin, as determined by micrococcal nuclease digestion. all of the major regions, including the transcriptionally active region of the genome, were found to be associated with nucleosomes. such a chromatin structure is likely to be an important element in the control of herpes simplex virus type 1 gene expression during latency. | 1989 | 2536115 |
| bile acid profile in a dog with cholangiocarcinoma. | | 1989 | 2536512 |
| new approaches to vaccine development. | a survey of current approaches to the development of vaccines against enteric diseases illuminates several strategies for the rational design of mycobacterial vaccines. one approach is to identify virulence factors and, through the use of transposon-mediated gene inactivation, to produce mutants that lack these factors. such a strategy can provide important information on antigens important to protection. genetic deletion or modification of these virulence factors provides insight into both the ... | 1989 | 2540520 |
| [changes in the functional activity of polymorphonuclear leukocytes in pseudotubercular infection]. | the functional activity of polymorphonuclear leukocytes (pnl) of the peripheral blood of rabbits infected orally with y. pseudotuberculosis was studied. by day 5 of y. pseudotuberculosis infection the pronounced stimulation of the myeloperoxidase system with the appearance of respiratory explosion was registered with the use of the nitro blue tetrazolium (nbt) test. the nbt-positive leukocyte index was found to increase 3.32-fold in comparison with the initial data. according to the results of t ... | 1989 | 2540602 |