| [mutual effect of some parasitic fungi, rhizobium, and other microorganisms of lens (lens esculenta moench) and the biological possibilities of protection]. | | 1970 | 5537326 |
| the nutrition of two strains of erwinia carotovora, causal organism of stalk rot of maize in india. | | 1970 | 5537391 |
| induction of nonpigmented variants of erwinia herbicola by incubation at supraoptimal temperatures. | several strains of erwinia herbicola produce white variants at high frequency when grown in yeast beef broth at 37 c. the relationships of the variants of e. herbicola y46 to the parent strain have been studied with respect to cultural, morphological, physiological and serological features. all the white variants are auxotrophic, showing a requirement for thiamine, and no instance of reversion was observed. representative variants show reactions similar to the parent strain on treatment with bot ... | 1971 | 5540997 |
| the asparaginase activities of bacteria. | | 1971 | 5556681 |
| microbial enzymes in clinical investigation, diagnosis and therapy. | | 1971 | 5556685 |
| [agents of new cucumber bacterioses]. | | 1971 | 5559342 |
| symposium on microbial changes in foods. bacterial spoilage of vegetables and certain fruits. | | 1971 | 5564385 |
| erwinia species from human sources. | | 1971 | 5567724 |
| [serological diagnostic method without isolation of erwinia amylovora culture from a diseased plant]. | | 1967 | 5611662 |
| [susceptibility of different varieties of lupine and other bean plants to lupine bacteriosis agents]. | | 1967 | 5618081 |
| [phytopathogenic bacteria from the genera pseudomonas and erwinia as bacteriosis agents of plants in the ukraine]. | | 1967 | 5618098 |
| [growth of pectolytic enzymes in continuous cultivation]. | | 1967 | 5619842 |
| extracellular and intracellular polygllacturonic acid trans-eliminases of erwinia carotovora. | | 1968 | 5642600 |
| cell division in a species of erwinia. x. morphology of the nuclear body in filaments produced by growth in the presence of d-serine. | | 1968 | 5646832 |
| cell division in erwinia: inhibition of nuclear body division in filaments grown in penicillin or mitomycin c. | low concentrations of penicillin or mitomycin c in cultures of erwinia sp. inhibit cell division. electron-microscopic analysis of serial sections of these nondividing cells reveals that division of the nuclear body is also inhibited. | 1968 | 5657062 |
| oligogalacturonide trans-eliminase of erwinia carotovora. | | 1968 | 5671040 |
| cell division in a species of erwinia. xi. some aspects of the carbon and nitrogen nutrition of erwinia species. | | 1968 | 5724891 |
| cell division in a species of erwinia. xii. a study of nutritional influences in d-serine inhibition of growth and division of erwinia sp., and of certain specific sites of d-serine action. | | 1968 | 5724892 |
| a disc electrophoretic comparison of protein patterns of erwinia amylovora with other bacteria, including associated yellow forms. | | 1968 | 5729094 |
| the influence of growth rate on cellular size of erwinia carotovora. | | 1968 | 5736944 |
| dna base composition and taxonomy of phyopathogenic and other enterobacteria. | | 1969 | 5787000 |
| l-asparaginase production by various bacteria. | of 123 species of bacteria surveyed for l-asparaginase synthesis, erwinia aroideae nrrl b-138 provided the highest yields. | 1969 | 5797949 |
| some observations on the physiology of erwinia herbicola and its possible implication as a factor antagonistic to erwinia amylovora in the "fire-blight" syndrome. | | 1969 | 5798523 |
| l-asparaginase production by erwinia aroideae. | maximum yields of 1,250 iu (international unit)/g (dry weight of cells) of l-asparaginase were obtained in 8 hr from erwinia aroideae nrrl b-138. partial purification and concentration of the extracted l-asparaginase yielded a preparation with an activity of 275 iu/ml. only one l-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a ph optimum of 7.5 and a k(m) of 3 x 10(-3) m. | 1969 | 5803630 |
| determination of phloroglucinol in the presence of phloretin using a modified vanillin reagent. | | 1969 | 5821305 |
| filamentous forms of erwinia amylovora. | | 1965 | 5827066 |
| cell division in a species of erwinia. ix. electron microscopy of normally dividing cells. | grula, e. a. (oklahoma state university, stillwater), and gerald l. smith. cell division in a species of erwinia. ix. electron microscopy of normally dividing cells. j. bacteriol. 90:1054-1058. 1965.-cells of an erwinia species (an enterobacteriaceae) divide by concomitant invagination of the cell wall and membrane. it is concluded that the process is initiated and sustained by the cell membrane. | 1965 | 5847795 |
| numerical survey of some bacterial taxa. | focht, d. d. (iowa state university, ames), and w. r. lockhart. numerical survey of some bacterial taxa. j. bacteriol. 90:1314-1319. 1965.-a numerical analysis was made of 77 properties of each of 43 bacterial strains, representing 25 genera from 8 families in the orders eubacteriales and pseudomonadales. four major groups were found, related to one another at approximately the same level of similarity: (1) a large cluster containing the subgroups (1a) athiorhodaceae-spirillaceae, (1b) xanthomon ... | 1965 | 5848329 |
| use of secondary data in numerical taxonomy of the genus erwinia. | lockhart, w. r. (iowa state university, ames), and karen koenig. use of secondary data in numerical taxonomy of the genus erwinia. j. bacteriol. 90:1638-1644. 1965.-numerical methods were used to study 34 bacterial cultures, including several species of erwinia and selected representatives of other genera of the enterobacteriaceae. separate computations were made by use of varying amounts of secondary data (i.e., information regarding descriptive or quantitative characters applicable only to org ... | 1965 | 5854588 |
| cell division in a species of erwinia. 8. amino acid composition of the mucopeptide in dividing and non-dividing cells. | | 1965 | 5861280 |
| effects of certain penicillins on growth and cell division in a species of erwinia. | | 1965 | 5866124 |
| [nucleotide composition of erwina caratovora f. citrullis resistant to streptomycin]. | | 1965 | 5872783 |
| classification of enterobacteria based on overall similarity. | krieg, r. e. (iowa state university, ames), and w. r. lockhart. classification of enterobacteria based on overall similarity. j. bacteriol. 92:1275-1280. 1966.-a numerical study was made of 53 organisms, representing 12 genera of the family enterobacteriaceae and 4 members of the genus aeromonas. a total of 105 features was determined for each culture. matching coefficients were computed, and the organisms were sorted into phenetic groups by use of the "highest-link" criterion. the genera entero ... | 1966 | 5924265 |
| polygalacturonase of erwinia carotovora. | | 1966 | 5954797 |
| [lipidolytic activity of some groups of gram-negative, nonsporulated bacteria from marine environment]. | | 1966 | 5970796 |
| [bacterial diseases of lupine seeds and sprouts in the ukraine]. | | 1966 | 5993604 |
| cell wall degradation by a pectate transeliminase. | | 1967 | 6032851 |
| identity of gram negative, yellow pigmented, fermentative bacteria isolated from plants and animals. | | 1967 | 6045100 |
| bacteriophage studies of isolates from fire blight sources. | | 1967 | 6064341 |
| inhibition of phenol oxidation by erwinia carotovora in potato tuber tissue and its significance in disease resistance. | | 1967 | 6077708 |
| molecular cloning of pectate lyase genes from erwinia chrysanthemi and their expression in escherichia coli. | a genomic library of erwinia chrysanthemi ec16 was constructed in plasmid phc79, and seven putative pectate lyase (pl) clones in escherichia coli were selected on pectate agar. six of the recombinant cosmids contained a common psti fragment of ca. 8.2 kilobases (kb). subcloning of this fragment in either orientation into the psti site of plasmid pbr329 resulted in e. coli transformants that produced a pl of pi 9.8 which was indistinguishable from one of two pls produced by strain ec16. a 6.6-kil ... | 1984 | 6090392 |
| cloning and characterization of reca genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r. | the reca genes of proteus vulgaris, erwinia carotovora, shigella flexneri and escherichia coli b/r have been isolated and introduced into escherichia coli k-12. all the heterologous genes restore resistance to killing by uv irradiation and the mutagen 4-nitroquinoline-1-oxide in reca- e. coli k-12 hosts. recombination proficiency is also restored as measured by formation of lac+ recombinants from duplicated mutant lacz genes and the ability to propagate phage lambda derivatives requiring host re ... | 1984 | 6090408 |
| [role of carbohydrate-protein recognition in the process of bdellovibrio attaching to bacterial host cells]. | the authors discuss the role of carbohydrates in the surface layer of the cell wall in the process of bdellovibrio attachment to host-bacteria cells. the paper presents the results of inhibitory analysis using sugars conducted with two model systems as well as the data about the effectiveness of the interaction between parasite cells and host cells after the modification of their surface polysaccharide layer with concanavalin a and sodium periodate. | 1984 | 6090874 |
| [interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)]. | gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ... | 1980 | 6104464 |
| 3''-phosphoryldihydrostreptomycin produced by the inactivating enzyme of erwinia carotovora. | | 1980 | 6154682 |
| serological relationships among flagella of erwinia carotovor var. atroseptica and some e. carotovora var. carotovora serogorups. | the 2 erwinia carotovora var. atroseptica serogroups and 2 out of 16 e. carotovora var. carotovora serogroups previously established on the basis of diffusible somatic antigens were shown to be serologically related by agglutination procedures using whole cells. the common agglutinating antigen in serogroups i, iii, v, and xviii was heat labile and identified as the bacterial flagella by the fluorescent antibody staining procedure. a few strains in serogorups i and iii apparently lacked flagella ... | 1980 | 6156748 |
| siderophore production by phytopathogenic microbial species. | | 1982 | 6218783 |
| production and modification of mu (g-) phage particles in e. coli k12 and erwinia. | | 1983 | 6222938 |
| mutagenesis of erwinia carotovora subsp. carotovora with bacteriophage mu d1 (apr lac cts62): construction of his-lac gene fusions. | the bacteriophage mu d1(apr lac cts62 ) obtained from an escherichia coli double lysogen carrying the defective mu d1 phage and a mu-p1 hybrid phage was utilized as a vector for phage mutagenesis in erwinia carotovora subsp. carotovora. among ampicillin-resistant transductants. 1.4% were auxotrophs. the synthesis of beta-galactosidase was derepressed upon starvation for histidine in two different his-lac fusion strains. | 1984 | 6233263 |
| comparison of the lipoprotein gene among the enterobacteriaceae. dna sequence of erwinia amylovora lipoprotein gene. | a dna sequence of 816 base pairs encompassing the entire erwinia amylovora lipoprotein gene was determined. sequence comparison between e. amylovora, escherichia coli, and serratia marcescens suggests that the structure of the lipoprotein has been highly conserved under the constraint of efficient gene expression selecting promoter structure, mrna secondary structure, and codon usage in addition to the polypeptide function. the sequence also suggests that the lpp gene of the three bacteria diver ... | 1981 | 6257705 |
| utilization of a thermosensitive episome bearing transposon tn10 to isolate hfr donor strains of erwinia carotovora subsp. chrysanthemi. | a thermosensitive episome bearing the transposon tn10, f(ts)::tn10 lac+, has been successfully transferred from escherichia coli to several wild strains of the enterobacteria erwinia carotovora subsp. chrysanthemi, which are pathogenic on saintpaulia ionantha. in one of these strains, all of the characters controlled by this episome (lac+, tetr, tra+) were expressed, and its replication was stopped at 40 degrees c and above. at 30 degrees c, the episome was easily transferred between strains der ... | 1982 | 6277860 |
| direct dna repeat in plasmid r68.45 is associated with deletion formation and concomitant loss of chromosome mobilization ability. | plasmid r68.45 has been useful in promoting the transfer of chromosomal markers in bacteria of many genera. in donors harboring r68.45, chromosome-mobilizing ability (cma) may be lost without the loss of other plasmid markers. however, cma can be somewhat stabilized by maintenance of the donors in the presence of kanamycin (km). we isolated variants of r68.45 from four bacterial species of three genera. plasmid variants isolated included those without cma, without transfer function (tra) and cma ... | 1982 | 6277862 |
| the chromosomal origin of replication (oric) of erwinia carotovora. | the chromosomal dna replication origin (oric) of the plant pathogen erwinia carotovora has been isolated and sequenced. the minimal e. carotovora oric regional functional in escherichia coli is a 374 base pair region located on a 7.9 kilobase pair sali fragment which also contains a functional asna gene. differences between the nucleotide sequence of the minimal origin regions of e. carotovora and those of e. coli and salmonella typhimurium are clustered nucleotide substitutions, with regions of ... | 1982 | 6281738 |
| [genetic transfer of pap38, pap39, and pap41 plasmids into e. coli, erwinia, and hafnia strains]. | genetic transfer factors pap38, pap39 and pap41 can be transferred to e. coli, both typed and untypecd, as well as to erwinia and hafnia strains, with different frequency (10(-2) to 10(-8)). the transconjugates thus obtained possess donor activity and can transfer the factors they have received to recipient e. coli strains. after transfer these factors are stably maintained in a new host for at least 10 days. under the action of ethidium bromide or elevated temperature the elimination of the tra ... | 1982 | 6295035 |
| comparison of the lipoprotein gene among the enterobacteriaceae. dna sequence of morganella morganii lipoprotein gene and its expression in escherichia coli. | a dna sequence of 532 base pairs encompassing the entire morganella morganii lipoprotein gene (lpp) was determined. sequence comparisons of the m. morganii lpp gene with the lpp genes from escherichia coli, serratia marcescens, and erwinia amylovora reveal that the m. morganii lpp gene is more distantly related to the e. coli lpp gene than any of the other lpp genes examined. between the e. coli and m. morganii lpp genes, the following homologies were found: 44% in the promoter region (bases, -4 ... | 1983 | 6305973 |
| transposon tn5 mutagenesis in erwinia carotovora subsp. carotovora and e. carotovora subsp. atroseptica. | in matings between escherichia coli 2492(pjb4ji) and erwinia carotovora subsp. carotovora ecc71 and e. carotovora subsp. atroseptica eca12, kmr gms transconjugants were obtained at high frequencies, indicating instability of the mu-containing plasmid pjb4ji and transposition of tn5 into the recipient genome. this was verified by southern blot hybridization with prz102 dna containing tn5 as the 32p-labeled probe. examination of kmr gms transconjugants of ecc71 and eca12 disclosed that a proportio ... | 1984 | 6321435 |
| histological and ultrastructural changes in the lungs of guinea pigs exposed to the extract of erwinia herbicola. | | 1984 | 6336494 |
| [localization of putrescine in erwinia carotovora atcc 15713 cells]. | | 1983 | 6336577 |
| kinetic analysis of hepatotoxicity associated with antineoplastic asparaginases. | | 1983 | 6339039 |
| rhizobium infection and nodulation: a beneficial plant disease? | | 1983 | 6357057 |
| changes in properties of phytopathogenic bacteria effected by plasmid prd1. | transfer of plasmid prd1 from escherichia coli k 12j62 -1 to phytopathogenic bacteria, agrobacterium tumefaciens, xanthomonas beticola and erwinia carotovora subsp. carotovora caused changes in conjugant properties not determined by the plasmid and the emergence of the properties not present in the parent strains. clones have been obtained with intermediate properties between donor and recipient, including those with altered or lost virulence. in transconjugants of a. tumefaciens virulence incre ... | 1984 | 6375619 |
| relationship between skin tests and bronchial provocation tests with allergens found in organic dusts in patients with bronchial asthma. | a group of 153 patients with bronchial asthma were subjected to bronchial provocation and intracutaneous tests with allergen from the gram negative bacterium erwinia herbicola which commonly occurs in organic dusts. of this group, 80 persons were also examined with both tests with the allergen of aspergillus fumigatus and 96 persons with the house dust extract. the frequency of positive skin reactions to the tested allergens was much greater than that of the positive bronchial reactions. no sign ... | 1984 | 6382985 |
| the inhibition of lymphocyte mitogenesis by asparaginase: a still unexplained phenomenon. | | 1984 | 6389170 |
| mutants of erwinia chrysanthemi defective in secretion of pectinase and cellulase. | erwinia chrysanthemi produced several pectate lyases (ec 4.2.2.2) and endocellulases (ec 3.2.1.4) which were largely secreted into the culture medium. mutants deficient in the secretion mechanism for these enzymes were obtained by chemical and insertion mutagenesis. further study of one such mutant revealed that both enzyme activities were retained simultaneously within the periplasmic space. | 1984 | 6389513 |
| conjugal transfer of e. coli f'lac from erwinia chrysanthemi to pseudomonas syringae pv. glycinea and the apparent stable incorporation of the plasmid into the pv. glycinea chromosome. | the e. coli f'lac plasmid was transferred from an erwinia chrysanthemi hfr8 donor to a multiply-auxotrophic, rifampicin-resistant pseudomonas syringae pv. glycinea recipient. transfer occurred at a frequency of approximately 10(-5)/donor. stable transconjugants which were able to utilize lactose as the sole carbon source after several transfers would not donate the f'lac plasmid in detectable frequency to other pv. glycinea or e. coli recipients. the plasmid dna was shown to be integrated into t ... | 1984 | 6394959 |
| cellobiose metabolism in erwinia: genetic study. | the study of mutants of erwinia specifically unable to ferment cellobiose indicates that the mutations are clustered between arg and ile on the chromosome of this organism. in vivo cloning of the genes responsible for cellobiose utilization lead to a plasmid, pbec2, which complements all erwinia clb- specific mutants. when introduced into wild-type e. coli it allows this organism to use cellobiose, arbutin and salicin; it also complements bglb and bglc mutants of escherichia coli indicating that ... | 1984 | 6396494 |
| [isolation of mutants of the erwinia carotovora temperate phage 59 under the action of n-methyl-n'-nitro-n-nitrosoguanidine]. | | 1983 | 6400791 |
| [prediction of microorganism resistance to the immobilization process in polyacrylamide gel]. | it is shown that the immobilization of bacterial cells in polyacrylamide gel or their exposure to monomer acrylamide results in a quantitatively similar decrease of their viability. it is indicated that acrylamide treatment may be used as a test for measuring the resistance of microbial populations to polyacrylamide gel immobilization and predicting the survival rate of microorganisms incorporated. | 1983 | 6410373 |
| chromosome transfer and r-prime plasmid formation mediated by plasmid pulb113 (rp4::mini-mu) in alcaligenes eutrophus ch34 and pseudomonas fluorescens 6.2. | plasmid pulb113 (rp4::mini-mu), which contains the mini-mu transposon, promoted both homologous and heterologous gene transfer from pseudomonas fluorescens 6.2 and alcaligenes eutrophus ch34. homologous gene transfer in p. fluorescens 6.2 and a. eutrophus ch34 occurred at a frequency of 10(-4) to 10(-5), and recombinants inherited unselected recessive markers, suggesting a process of chromosome mobilization. loci involved in autotrophic growth were among those transferred in a. eutrophus. in het ... | 1983 | 6411681 |
| enzymatic synthesis of phenoxymethylpenicillin using erwinia aroideae enzyme. | enzymatic synthesis of phenoxymethylpenicillin from 6-aminopenicillanic acid and phenoxyacetic acid methyl ester was attempted by using partially purified alpha-acylamino-beta-lactam acylhydrolase i (alahase i) enzyme from erwinia aroideae nrrl b-138. the reaction rates were carefully followed by determination of 6-aminopenicillanic acid (6-apa), phenoxymethylpenicillin (pnv), phenoxyacetic acid (poa), phenoxyacetic acid methyl ester (pom), and phenoxyacetylglycine (pog) using high performance l ... | 1984 | 6438038 |
| conservation and variation of nucleotide sequences within related bacterial genomes: enterobacteria. | we have assessed the degree of relatedness of several portions of the escherichia coli genome to the corresponding portions of the genomes of representative enteric bacteria, using the southern transfer and hybridization technique (e. southern, j. mol. biol. 98:503-517, 1975). the degree of relatedness varied among the regions examined. judging both by the relative amounts of deoxyribonucleic acid in the various enteric genomes that are highly homologous and by the conservation of positions of r ... | 1980 | 6447143 |
| introduction of bacteriophage mu into bacteria of various genera and intergeneric gene transfer by rp4::mu. | the host range of coliphage mu was greatly expanded to various genera of gram-negative bacteria by using the hybrid plasmic rp4::mu cts, which is temperature sensitive and which confers resistance to ampicillin, kanamycin, and tetracycline. these drug resistance genes were transferred from escherichia coli to members of the general klebsiella, enterobacter, citrobacter, salmonella, proteus, erwinia, serratia, alcaligenes, agrobacterium, rhizobium, pseudomonas, acetobacter, and bacillus. mu phage ... | 1981 | 6450749 |
| bacterial survival in a dilute environment. | bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. this approach permitted separation of the lake isolates into two categories. members of the tribe klebsielleae were shown to have a prolonged survival rate of 40% or better after 24 h, whereas nonsurvivors were not viable for much longer than 24 ... | 1981 | 6454390 |
| [homology of the temperate viruses of a polylysogenic culture of erwinia carotova 268]. | | 1984 | 6499646 |
| isolation of l-cycloserine from erwinia uredovora. | an antibiotic which seems to be a cell wall synthesis-inhibitor was isolated from a bacteria strain identified as erwinia uredovora. the antibiotic was identified with l-cycloserine from its physico-chemical properties. this is the first example for isolation of l-cycloserine as a microbial product. | 1984 | 6501090 |
| utilization of plasmid pulb113 (rp4::mini-mu) to construct a linkage map of erwinia carotovora subsp. chrysanthemi. | we report experimental evidence that pulb113, an rp4::mini-mu plasmid, mediates chromosome transfer in a strain of erwinia carotovora subsp. chrysanthemi which does not accept the f episome. this allowed us to construct a genetic map of that strain by measuring the frequencies of cotransfer of different markers (thy, leu, pro, [his, trp], thya, rpsl, ile). | 1983 | 6574127 |
| the effect of freeze-drying on the quaternary structure of l-asparaginase from erwinia carotovora. | l-asparaginase (l-asparagine amidohydrolase, ec 3.5.1.1) from erwinia carotovora undergoes extensive dissociation from active tetramer to inactive monomers when freeze-dried. the monomeric state is stabilized by reconstitution of the freeze-dried enzyme with buffers of high ph and high ionic strength. some compounds, particularly sugars and sugar derivatives, prevent dissociation on freeze-drying, whereas others, such as urea and chaotropic ions, increase dissociation. the effects of additives a ... | 1983 | 6652094 |
| fluoride-induced filaments of erwinia carotovora. | sodium fluoride induces filamentous growth of erwinia carotovora when it is grown in liquid media containing aspartic acid only as the sole source of nitrogen. it is proposed that a stable complex of f(-)-mg2+-enzyme and po2(4) resulting in mg2+ deficiency and consequent inability of e. carotovora cells to oxidize aspartic acid normally, is responsible for the formation of filaments in the presence of fluoride ions. | 1983 | 6684838 |
| [enterobacter agglomerans bacteremia (erwinia herbicola)]. | | 1984 | 6727463 |
| the extraction and mechanism of a novel isomaltulose-synthesizing enzyme from erwinia rhapontici. | the single enzyme that mediates the bioconversion is demonstrated to be located in the cells' periplasmic space, a site that facilitates its use as an industrial biocatalyst, and to be a previously undescribed hexosyltransferase with four novel features. the enzyme is sucrose-specific, and has an intramolecular mechanism in which both glucose and fructose residues appear to be enzyme-bound. thirdly, it is reaction-non-selective, forming simultaneously isomaltulose and a second hitherto uncharact ... | 1984 | 6743261 |
| control of ph in liquid cultures of microorganisms with an ion-exchange resin. | | 1982 | 6754043 |
| [conjugational transfer of chromosomal markers in the erwinia chrysanthemi bacterial system. ii. characteristics of the donor strain of erwinia chrysanthemi vy1-10]. | some properties of the donor erwinia chrysanthemi vy1-10 strain are similar to those of hfr donors of escherichia coli. these are stable inheritance of the flac plasmid and the capacity for linear and polarized transfer of the chromosome from one orit site in the following order: o ... thr, leu ... pro ... his. in addition to the ability to transfer its own chromosomal markers, the donor e. chrysanthemi vy1-10 also transfers lac+ marker during initial conjugational steps. the lac+ marker can be ... | 1982 | 6759307 |
| [metabolism of dicarboxylic amino acids and their amides in microorganisms of the enteric group]. | | 1980 | 6778037 |
| improvement in the persistence of microbial asparaginase and glutaminase in the circulation of the rat by chemical modifications. | three enzymes used in cancer chemotherapy (asparaginases from escherichia coli and erwinia carotovora and glutaminase from achromobacter) were each reacted with four amino specific reagents (ethyl acetimidate, o-methylisourea, succinic anhydride, and formaldehyde/sodium borohydride). the half-lives of the modified enzymes measured in the blood of rats showed that guanidation, acetimidation and reductive alkylation were more likely to increase the persistence of the native enzymes than succinylat ... | 1981 | 6794649 |
| membrane-bound penicillinases in gram-positive bacteria. | the penicillinases of bacillus licheniformis, bacillus cereus, and staphylococcus aureus are related in structure and cellular localization to one another more closely than they are to penicillinases from gram-negative organisms. in the latter, penicillinases are almost exclusively found in the periplasm, while the gram-positive bacteria retain a substantial proportion as hydrophobic membrane-bound forms. we recently showed (nielsen, j. b. k., caulfield, m. p., and lampen, j. o. (1981) proc. nat ... | 1982 | 6802832 |
| hand carriage of aerobic gram-negative rods may not be transient. | in order to determine whether hand carriage of aerobic gram-negative rods is continuous we used the glove-handwash technique to sample the hands of two groups (four each) of health care workers with normal hands (surgical intensive care unit, medical ward) and one group (four) with hand dermatitis (hd) and a group (five) of control subjects -- secretaries with no exposure to patients. each subject was sampled repeatedly over three to six weeks. the mean number of samples for each group was 25.2, ... | 1982 | 6808057 |
| evaluation of three differential media for detection of enterobacter agglomerans (erwinia herbicola). | dextrin-fuchsine-sulfite medium (dfs), rimler-shotts agar (rs), and a new lysine-ornithine-mannitol agar (lom) were tested for detection of enterobacter agglomerans. in human stools, lom and dfs were most sensitive at coliform-to-e. agglomerans ratios of less than or equal to 10(2) and e. agglomerans inocula of greater than 10(2) per plate. both lom and dfs detected one strain in 254 stools, but rs proved to be inhibitory. | 1982 | 6809787 |
| enzyme therapy: problems and solutions. | | 1982 | 6810751 |
| aldohexuronate transport system in erwinia carotovora. | the biochemical and physiological aspects of hexuronate transport in erwinia carotovora were studied to approach the genetic regulation of the hexuronate degradative pathway in this bacterial species. an active transport system for glucuronate and galacturonate uptake exists in e. carotovora. the glucuronate entry reaction displayed saturation kinetics with an apparent km of 0.05 mm (at 25 degrees c; ph 7). galacturonate appeared to be a competitive inhibitor of glucuronate uptake with a ki of 0 ... | 1983 | 6841313 |
| ice nucleating activity of pseudomonas syringae and erwinia herbicola. | chemical and biological properties of the ice nucleating sites of pseudomonas syringae, strain c-9, and erwinia herbicola have been characterized. the ice nucleating activity (ina) for both bacteria was unchanged in buffers ranging from ph 5.0 to 9.2, suggesting that there were no essential groups for which a change in charge in this range was critical. the ina of both bacteria was also unaffected by the addition of metal chelating compounds. borate compounds and certain lectins markedly inhibit ... | 1983 | 6848483 |
| in vivo cloning of erwinia carotovora genes involved in the catabolism of hexuronates. | using the rp4::mini-mu pulb113 plasmid, an rp4 derivative carrying a deleted mu prophage which allows the plasmid to pick up any chromosomal dna segment to form r' plasmids, we cloned all of the genes of erwinia carotovora involved in the catabolism of the hexuronates and in the transport of these substrates. with the r' plasmids we isolated, we performed complementation analysis and found that, in the erwinia carotovora strain we used, the genes involved in the catabolism of the hexuronates are ... | 1983 | 6853444 |
| structure of erwinia carotovora temperate bacteriophage 59 and its dna. | the temperate phage 59 from erwinia carotovora and its dna were studied. the phage particles have an icosahedral head and a long noncontractile tail with a base plate. the virus dna makes up 50% of the total virus and exists as a linear molecule (molecular weight, 2.6 x 10(7)). a model of virus structural organization is presented. | 1983 | 6854737 |
| l-asparaginase pharmacokinetics and asparagine levels in cerebrospinal fluid of rhesus monkeys and humans. | l-asparaginase has been widely used for the treatment of acute lymphoblastic leukemia. therapeutic and toxic effects in the central nervous system have been noted with systemic treatment. in order to better define the relationship between l-asparaginase administration and cerebrospinal fluid (csf) asparagine levels, l-asparaginase and asparagine were measured in the csf of rhesus monkeys following intrathecal and i.v. administration. following intrathecal injection, the enzyme activity of escher ... | 1981 | 6895481 |
| plasmid pea566 from erwinia aroideae. | a new plasmid designated pea566 was isolated from erwinia aroideae. the molecular weight of the plasmid, as determined by neutral and alkaline sucrose gradient centrifugation, electron microscopy, and agarose gel electrophoresis, was 6.6 x 10(6). the plasmid replicated under relaxed control, had three cleavage sites for kpni restriction endonuclease, and no sites for ecori, bamhi, sali, psti, and hindiii. | 1980 | 6927815 |
| generalized transduction in the enterobacterial phytopathogen erwinia chrysanthemi. | bacteriophages induced by mitomycin treatment of erwinia chrysanthemi ks612 produced plaques on lawns of e. chrysanthemi ec183 and ks605. bacteriophage erch-12, purified from one such plaque, transferred an array of chromosomal genes (arg, leu, his, ser, thr, trp, ura) to appropriate recipient strains derived from e. chrysanthemi ec 183. recombinants were formed in the absence of cellular contact between donor and recipient bacteria and in the presence of deoxyribonuclease. ultraviolet irradiati ... | 1980 | 6931829 |
| plasmid colvbtrp maintenance in erwinia carotovora. | plasmid colvbtrp maintenance in erwinia carotovora cells was followed by measuring kinetics of elimination of plasmid genetic markers and loss of plasmid deoxyribonucleic acid. an e. carotovora mutant stably carrying plasmid colvbtrp was isolated. besides stable plasmid maintenance, the mutant showed altered sensitivity to male-specific phage ms2, sensitivity to drugs, and colony morphology. | 1981 | 6944305 |
| distribution of coenzyme b12-dependent diol dehydratase and glycerol dehydratase in selected genera of enterobacteriaceae and propionibacteriaceae. | the presence of diol dehydratase and glycerol dehydratase was shown in several bacteria of enterobacteriaceae grown anaerobically on 1,2-propanediol and on glycerol, respectively. diol dehydratases of enterobacteriaceae were immunologically similar, but distinct from that of propionibacterium freudenreichii. | 1980 | 6988416 |
| acceptance by erwinia spp. of r plasmid r68.45 and its ability to mobilize the chromosome of erwinia chrysanthemi. | r plasmid r68.45 was transferred in broth matings from escherichia coli to strains of erwinia amylovora, e. carotovora subsp. atroseptica, e. chrysanthemi, and e. herbicola (enterobacter agglomerans); the frequency of transfer ranged from 2 x 10(-8) to 5 x 10(-4) per input donor cell depending on the bacterial species. the drug resistance markers tet(+), amp(+), and kan(+) were stable in these erwinia species. transconjugants of erwinia spp., but not of the wild-type parent erwinia strains, acqu ... | 1980 | 6989797 |
| phosphatidylethanolamine distribution and fluidity in outer and inner membranes of the gram-negative bacterium erwinia carotovora. | 1. the distribution of phosphatidylethanolamine, the major lipid of erwinia carotovora, was investigated in intact bacteria, spheroplasts and outer- and inner-membrane preparations, with the amino-group reagent 2,4,6-trinitrobenzenesulphonic acid. only 4% was found on the external surface of the outer membrane with 30% on the internal surface, whereas the inner membrane had 27 and 38% on its external and internal surfaces respectively. some comparative studies were made with three other bacteri ... | 1980 | 6996674 |
| expression of klebsiella his and nif genes in serratia marcescens, erwinia herbicola and proteus mirabilis. | plasmid prd1, an r plasmid of the p incompatibility group which carries his and nif genes from klebsiella pneumoniae in addition to drug resistance markers derived from rp4, was transferred to his- mutants of serratia marcescens, erwinia herbicola and proteus mirabilis. his+ transconjugants were obtained at low but different frequencies according to recipient genus. transconjugants all acquired the drug resistance, and were nif+ in s. marcescens and e. herbicola, having acetylene-reducing activi ... | 1980 | 7000024 |