| facilitative actions of the protein kinase-c effector system on hormonally stimulated adenosine 3',5'-monophosphate production by swine luteal cells. | the exact nature of the interaction(s) between camp and calcium-sensitive phospholipid-dependent protein kinase-c effector pathways is not well understood in many tissues, including the ovary. in the present work we have evaluated the ability of protein kinase-c to modulate receptor-and nonreceptor-mediated camp generation in acute suspension cultures of swine luteal cells. cells were exposed to lh (1 micrograms/ml), forskolin (100 microm), cholera toxin (1 microgram/ml), pertussis toxin (100 ng ... | 1989 | 2551649 |
| cloning and expression of mouse-brain calmodulin as an activator of bordetella pertussis adenylate cyclase in escherichia coli. | cloning of higher eukaryotic genes has seldom been performed by complementation of a defective prokaryotic function. this is especially true in the case of functions that are normally absent from the prokaryotic host. we demonstrate here that it is possible to identify by complementation the cdna from mouse brain, which encodes calmodulin (cam) synthesis, in spite of the fact that the recipient strain, escherichia coli, does not normally harbour a cam function. a three-component cloning procedur ... | 1989 | 2551780 |
| analysis of the chromosomal location of two copies of a bordetella pertussis insertion sequence. | is481v1 and is481v2 are two copies of a bordetella pertussis insertion sequence element. we have shown that is481v1 is located within 3 kbp of the start of the adenylate cyclase gene whilst is481v2 is immediately adjacent to the end of the agglutinogen 2 gene and provides the stop codon for that gene. in addition, is481v1 and is481v2 were present at these two specific sites in nine strains of b. pertussis, including two phase iv strains which expressed neither adenylate cyclase nor agglutinogen ... | 1989 | 2552259 |
| [isolation of hemolytic cultures of bordetella pertussis intended for the production of acellular pertussis preparations and the characterization of their biological properties]. | the heterogeneity of the population of b. pertussis laboratory strains with respect to the capacity of individual clones to lyse erythrocytes has been established. the complete or partial reduction of the antigens under study in nonhemolytic bacteria has been shown. the use of hemolytic cultures for the preparation of acellular pertussis vaccine makes it possible to increase the content of b. pertussis toxin in these vaccines. | 1989 | 2552711 |
| gtp gamma s increases thrombin-mediated inositol trisphosphate accumulation in permeabilized human endothelial cells. | ca2+-mobilizing agonists stimulate phospholipase c-mediated phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol trisphosphate (ip3) formation in pulmonary as well as in peripheral vascular endothelial cells (ec). in general, it is believed that receptor-phospholipase c interactions involve a guanine nucleotide regulatory (g) protein. this interaction can be inhibited by bordetella pertussis toxin in certain cells. here we report that pertussis toxin catalyzes the [32p]adp ribosylation ... | 1989 | 2552882 |
| the bvga gene of bordetella pertussis encodes a transcriptional activator required for coordinate regulation of several virulence genes. | the bvg region of the respiratory pathogen bordetella pertussis coordinately regulates the expression of several unlinked virulence determinants in response to environmental signals. the dna sequence of the bvg region contains three genes (bvga, bvgb, and bvgc). transcription of a single-copy fusion consisting of the upstream region of a bvg-activated b. pertussis gene (fhab) attached to the promoterless lac operon in escherichia coli requires the entire bvgabc region in trans. activation of the ... | 1989 | 2553677 |
| analysis of bordetella pertussis virulence gene regulation by use of transcriptional fusions in escherichia coli. | the virulence regulon of bordetella pertussis includes a trans-acting regulatory locus, bvg, that is required for expression of several virulence factors. the virulence control system also responds to environmental signals. we have reconstructed a bvg-dependent regulatory system in escherichia coli by using bacteriophage lambda vectors carrying transcriptional fusions to laczya. single-copy laczya fusions to the b. pertussis fhab locus, which encodes the attachment factor filamentous hemagglutin ... | 1989 | 2553678 |
| adenylate cyclase toxin from bordetella pertussis. identification and purification of the holotoxin molecule. | bordetella pertussis adenylate cyclase (ac) toxin is a calmodulin-activated adenylate cyclase enzyme which has the capacity to enter eukaryotic target cells and catalyze the conversion of endogenous atp into cyclic amp. in this work, the ac holotoxin molecule is identified and isolated. it is a single polypeptide of apparent 216 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. monoclonal antibodies which immunoprecipitate ac activity from extracts of wild type b. p ... | 1989 | 2553737 |
| pertussis (whooping cough) toxin and bordetella pertussis whole-cell antibody levels in a healthy new zealand population. | enzyme linked immunosorbent assay (elisa) tests were used to measure igg antibody levels to bordetella pertussis whole-cell and to pertussis toxin in a healthy new zealand population. the percentage of individuals with measurable antibody to b pertussis whole-cell increased from 47.3% in the 5 year olds to 93.0% in the 40-49 age group, from which the percentage with antibody dropped to 88.7% in the 50-65 age group. the percentage of persons with antibody to the toxin increased with age from 17.5 ... | 1989 | 2554227 |
| a unique sequence of the bordetella pertussis toxin operon. | | 1989 | 2554254 |
| pertussis holotoxoid formed in vitro with a genetically deactivated s1 subunit. | the cytotoxicity of pertussis toxin, a multisubunit exotoxin produced by bordetella pertussis, is believed to be due to the adp-ribosyltransferase activity of the s1 subunit. we have previously described the recombinant expression of each of the five individual pertussis toxin subunits in escherichia coli and the production of an enzymatically deficient form of the s1 subunit by site-directed mutagenesis. we now report the in vitro assembly of holotoxin from native pertussis toxin b oligomer and ... | 1989 | 2554311 |
| bordetella pertussis adenylate cyclase inactivation by the host cell. | bordetella pertussis produces a calmodulin-dependent adenylate cyclase (ac) which acts as a toxin capable of penetrating eukaryotic cells and generating high levels of intracellular cyclic amp. transfer of target cells into b. pertussis ac-free medium leads to a rapid decay in the intracellular ac activity, implying that the invasive enzyme is unstable in the host cytoplasm. we report here that treatment of human lymphocytes with a glycolysis inhibitor and an uncoupler of oxidative phosphorylati ... | 1989 | 2554887 |
| bordetella pertussis adenylate cyclase: purification and characterization of the toxic form of the enzyme. | bordetella pertussis produces a calmodulin-sensitive adenylate cyclase (ac) which is an essential virulence factor in mammalian pertussis. here we report the purification and characterization of the toxic form of the enzyme, which penetrates eukaryotic cells and generates high levels of intracellular camp. this form was purified from an extract of b.pertussis strain carrying a recombinant plasmid which over-produced both enzymatic and toxic activities of the enzyme. western blot analysis of the ... | 1989 | 2555185 |
| preparation of filamentous hemagglutinin from bordetella pertussis and assay for serum antibodies to filamentous hemagglutinin and pertussis toxin for clinical and public health laboratories. | a procedure that is sufficiently simple and economical for use in clinical and public health laboratories for producing and purifying filamentous hemagglutinin (fha) and determining antibodies to this major antigen of bordetella pertussis in serum is described. high yields of fha (40 to 80 mg/liter) were obtained in the supernatant by cultivating b. pertussis in modified cl medium. the fha antigen was separated from pertussis toxin (pt) and other antigens by chromatography on hydroxylapatite. re ... | 1989 | 2556434 |
| isolation of a protein fraction from bordetella pertussis that facilitates entry of the calmodulin-sensitive adenylate cyclase into animal cells. | bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium. several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular camp levels [confer, d. l., & eaton, j. w. (1982) science 217, 948-950; shattuck, r. l., & storm, d. r. (1985) biochemistry 24,6323-6328]. however, the mechanism for entry of the catalytic subunit of the ade ... | 1989 | 2557896 |
| effect of adjuvants on igg and ige response to ovalbumin in rats. | specific and total igg and ige serum levels were measured in sprague-dawley rats after immunization with ovalbumin (oa). oa was administered in an aqueous solution, suspended in alum or in complete freund's adjuvant (cfa) with or without simultaneous administration of bordetella pertussis (bp). control groups received only saline, alum or cfa. specific igg, total igg and total ige were determined by elisa methods. specific ige was biologically evaluated. the highest specific igg and ige antibody ... | 1989 | 2558566 |
| cloning of the virulence regulatory (vir) locus of bordetella pertussis and its expression in b. bronchiseptica. | hybridisation with cosmid prmb2, containing the vir locus of bordetella pertussis, showed that the tn5 insertion in b. pertussis bp347 (vir-) was located with 1 2.7 kb ecori fragment. when subcloned, this fragment alone was unable to complement bp347, but a larger 8.0 kb region which included the 2.7 kb ecori fragment, did restore expression of virulence associated properties to bp347, and to avirulent phase variants of both b. pertussis and b. bronchiseptica. ecori-digested dna from stains of a ... | 1989 | 2558953 |
| the effect of cyclodextrin on lipopolysaccharide production in cultures of bordetella pertussis. | the effect of adding 500 micrograms of (2,6-0-dimethyl) beta-cyclodextrin (me-beta-cd) per ml of stainer-scholte (ss) medium in two-day shaker flask cultures of bordetella pertussis on the production of lipopolysaccharide (lps) was investigated. the amount of lps per 10(9) cells found in the supernatants of these cultures was either somewhat reduced or unaffected by comparison with the amounts in cultures grown in ss-medium alone. in addition, the time course of lps release from cultures of b. p ... | 1989 | 2559082 |
| analysis of separate isolates of bordetella pertussis repeated dna sequences. | two independent isolates of a bordetella pertussis repeated dna unit were sequenced and shown to be an insertion sequence element with five nucleotide differences between the two copies. the sequences were 1053 bp in length with near-perfect terminal inverted repeats of 28 bp, had three open reading frames, and were each flanked by short direct repeats. the two insertion sequences showed considerable homology to two other b. pertussis repeated dna sequences reported recently: is481 and a 530 bp ... | 1989 | 2559151 |
| phase variants of bordetella bronchiseptica arise by spontaneous deletions in the vir locus. | bordetella bronchiseptica is a common respiratory tract pathogen of many mammalian species. nucleotide sequences from the locus involved in coordinate regulation of b. pertussis virulence factors, vir, were shown to have a high degree of homology to chromosomal dna from virulent (vir+) and avirulent (vir-) strains of b. bronchiseptica. small deletions, 50 bp to 500 bp, within the vir locus were found in some of the vir- phase variants. the vir locus and the adjacent 5' portion of the fhab struct ... | 1989 | 2560120 |
| specific identification of bordetella pertussis by the polymerase chain reaction. | oligonucleotide primers were used to amplify specific dna regions of the bordetella pertussis genome by the polymerase chain reaction. one pair of primers, ptp1/ptp2, identified a 191-bp dna fragment located in the regulatory region of the pertussis toxin operon; a second pair of primers led to amplification of a 121-bp dna piece located in an insertion-like element specific to b. pertussis. both sets of primers were able to discriminate between the pathogen and related bordetella species; they ... | 1989 | 2560238 |
| invasive adenylate cyclase toxin of bordetella pertussis. | bordetella pertussis produces an adenylate cyclase which is a toxin. the enzyme penetrates eukaryotic cells and, upon activation by host calmodulin, generates high levels of intracellular camp; as a result bactericidal functions of immune effector cells are considerably impaired. the toxin is composed of a single polypeptide that possesses both the catalytic and the toxic functions. it penetrates the host cell directly from the plasma membrane and is concomitantly inactivated by a proteolytic de ... | 1989 | 2560273 |
| prevention of pertussis. | pertussis (whooping cough) is an acute respiratory disease caused by bordetella pertussis. it occurs worldwide and is an important cause of morbidity and mortality in areas where immunization rates are low, particularly among children less than 1 year of age. the characteristic presentation of pertussis is paroxysmal coughing followed by a long inspiratory effort that produces the classic whoop. lymphocytosis is frequently present. complications include pneumonia and seizures secondary to hypoxi ... | 1989 | 2560581 |
| specific ige-antibodies to bordetella pertussis antigens during whooping cough. | | 1989 | 2564540 |
| [use of an immunoblotting method for identifying the individual proteins in the antigenic complexes of bordetella pertussis]. | the composition of antigenic complexes isolated from the supernatant fluid of b. pertussis culture has been studied by means of immunoblotting techniques. in preparations obtained from b. pertussis strains 305 and 475 fragments of the molecule of fimbrial hemagglutinin, three subunits of b. pertussis toxin and agglutinogens 2 and 3 have been detected with the use of antisera to b. pertussis protective substances. | 1989 | 2568040 |
| use of the promoter fusion transposon tn5 lac to identify mutations in bordetella pertussis vir-regulated genes. | mutants of bordetella pertussis deficient in virulence-associated factors were identified by using the transposon tn5 lac. tn5 lac is a derivative of tn5 which generates promoter fusions for beta-galactosidase. tn5 lac insertions in the vir-regulated genes of b. pertussis were identified by selecting for kanamycin-resistant mutants that expressed beta-galactosidase when the vir-regulated genes were expressed but not when the vir-regulated genes were turned off. fourteen different mutations in vi ... | 1989 | 2569447 |
| lethal infection by bordetella pertussis mutants in the infant mouse model. | different aspects of lethal infection of infant mice with bordetella pertussis were examined. mutants deficient in vir-regulated genes were tested for the ability to cause a lethal infection in the infant mouse model. adenylate cyclase toxin-hemolysin and pertussis toxin were required to cause a lethal infection at low doses. mixed infection caused by challenging the mice with an equal number of pertussis toxin and adenylate cyclase toxin-hemolysin mutants at a dose at which neither alone was le ... | 1989 | 2572561 |
| serum igg, iga, and igm responses to pertussis toxin, filamentous hemagglutinin, and agglutinogens 2 and 3 after infection with bordetella pertussis and immunization with whole-cell pertussis vaccine. | class antibody responses to pertussis toxin (pt), filamentous hemagglutinin (fha), and agglutinogens 2 and 3 (agg 2/3) were studied in 114 patients with pertussis, 49 family contacts of patients with pertussis, and 139 infants attending vaccination clinics. igg responses to fha and pt were similar in patients with pertussis and in infants immunized three times with whole-cell pertussis vaccine. iga responses to fha and pt were greater in patients with pertussis than in vaccines, and a serum log1 ... | 1989 | 2572651 |
| serospecific protection of mice against intranasal infection with bordetella pertussis. | the ability of purified serospecific agglutinogens from bordetella pertussis to protect mice against intranasal infection has been examined. immunization with agglutinogen 2 protected mice against infection with 1.2.0 or 1.2.3 serotypes of b. pertussis, whereas immunization with agglutinogen 3 protected mice against infection with all serotypes. more importantly immunization with serospecific agglutinogen resulted in immune selection so that organisms recovered following infection did not expres ... | 1989 | 2573215 |
| serotyping bordetella pertussis strains. | the serotyping scheme for bordetella pertussis, developed in the 1950s, depends on the presence or absence of various strains of three major agglutinogens, two of which have been shown to be fimbrial proteins, and several minor agglutinogens, the biochemical nature of which is unknown. this article reviews the reasons for the confusion which has recently arisen over the nomenclature of the serotype antigens and proposes a simplified serotyping scheme based on the fimbrial components. | 1989 | 2575308 |
| release of histamine and arachidonate from mouse mast cells induced by glycosylation-enhancing factor and bradykinin. | stimulation of normal rat splenic t cells with pertussigen (lymphocytosis-promoting factor from bordetella pertussis) resulted in the release of a soluble factor that enhanced the assembly of n-linked oligosaccharides to ige-binding factors during their biosynthesis. the glycosylation-enhancing factor (gef) is a kallikrein-like enzyme and is purified by absorption to p-aminobenzamidine-agarose followed by elution with benzamidine. incubation of normal mouse mast cells with affinity-purified gef ... | 1985 | 2578523 |
| protective effects of human gamma-globulin preparations against experimental aerosol infections of mice with bordetella pertussis. | the protective effects of three types of pooled human gamma-globulin preparations (intact: gg, s-sulfonated: ggs, and pepsin-treated: ggp) for intravenous use against experimental aerosol infection of mice with bordetella pertussis have been evaluated. the gamma-globulin preparations gg and ggs showed significant protective activity whereas ggp did not, as evaluated by survival numbers, body weight gains and suppression of leucocytosis. gg and ggs but not ggp also possessed neutralizing activity ... | 1985 | 2578698 |
| acute experimental allergic encephalomyelitis in the mouse: immunopathology of the developing lesion. | to investigate the sequence of immunopathologic events during lesion formation in acute experimental allergic encephalomyelitis (eae), sjl/j mice were inoculated with isogeneic spinal cord in complete freund's adjuvant (cfa) and with bordetella pertussis on days 1 and 3 postinoculation (pi). mice were sampled at different time points pi and t cells, t-cell subsets. ia+ cells, ig+ cells, albumin, and ig deposits were localized in frozen sections by the avidin-biotin complex (abc) method and direc ... | 1985 | 2578895 |
| the effect of the castor bean toxin, ricin, on rat ige and igg responses. | ige responses are closely regulated in non-atopic humans and low ige responder animals. after an initial period of ige production following antigen exposure, ige synthesis appears to be actively suppressed. inhalation of the dust of castor beans induces persistent ige responses in atopic and non-atopic humans alike. this phenomenon was investigated in animals. hooded lister rats were immunized intraperitoneally with different preparations of castor bean. these had been heated for different lengt ... | 1989 | 2592006 |
| the effect of disodium 4-chloro-2-iminodibenzoate (cca) on ige levels and anaphylactic shock. | the effect of disodium 4-chloro-2,2-iminodibenzoate (cca) on ige antibody response was examined in c3h/a and (balb/c x c57bl/6j) f1 hybrid mice immunized with low doses of ovalbumin (oa) adsorbed on aluminium hydroxide gel. cca administered orally at the doses of 5 and 50 mg/kg/day reduced ige antibody production in these mice as determined by pca test. high doses of cca (100 mg/kg/day) given from day 7 before immunization of c57bl mice and during 1 week after immunization of mice with oa and bo ... | 1989 | 2596370 |
| inhibition of lymphocyte circulation in mice by pertussis toxin. | pertussis toxin (pt), a protein toxin of bordetella pertussis, also called pertussigen, has a wide range of biological activities, including the induction of lymphocytosis. this phenomenon was investigated by studying lymphocyte circulation in mice. lymph node cell suspensions were exposed to pt in vitro and then injected intravenously. a double radiolabel technique was employed, in which pt-treated and control cells were injected into the same animals. the protocol used in these experiments was ... | 1989 | 2613276 |
| hypothermia due to an ascending impairment of shivering in hyperacute experimental allergic encephalomyelitis in the lewis rat. | severe hypothermia and an ascending impairment of shivering are previously undescribed clinical signs in hyperacute experimental allergic encephalomyelitis (eae) in the lewis rat. these occurred in hyperacute eae induced by inoculation with guinea pig spinal cord homogenate and heat-killed bordetella pertussis. hypothermia was first detected on day 6-7 post-inoculation, within 12-24 h of the onset of neurological signs, and became more severe as the disease progressed. rectal temperatures less t ... | 1989 | 2614469 |
| bordetella adenylate cyclase is a virulence associated factor and an immunoprotective antigen. | bordetella pertussis and bordetella parapertussis are both causative agents of whooping cough outbreaks. although not expressing the pertussis toxin, b. parapertussis induces, in a murine model, an acute hemorrhagic edematous alveolitis, similar to that observed with b. pertussis. these data suggest that the pertussis toxin may only play an accessory role in the acute pulmonary syndrome observed during bordetella infection. both with b. pertussis and b. parapertussis, the ability to induce letha ... | 1989 | 2622329 |
| human cellular immune responses to bordetella pertussis infection. | we have compared the responses of peripheral blood leucocytes from three groups (i) patients suffering from pertussis (whooping cough), (ii) clinical staff caring for those patients and laboratory staff working with bordetella pertussis, and (iii) staff with no known recent contact with b. pertussis. in vitro stimulation with filamentous haemagglutinin (fha) caused significant increases in proliferation of only the patient group's lymphocytes. in vitro stimulation with pertussis toxin (pt) cause ... | 1989 | 2629905 |
| antigen-induced lung solute clearance in rats is dependent on capsaicin-sensitive nerves. | chemosensitive sensory nerves have an important effector role in the control of vascular permeability in rat airways after neurogenic inflammation. to investigate whether they also have a role in antigen-induced lung inflammation, we have studied the changes in lung solute clearance (lsc) in sensitized rats after aerosol challenge with allergen and the effect of prior capsaicin-induced denervation on these changes. sprague-dawley rats were immunized with egg albumin (ea), using aluminum hydroxid ... | 1989 | 2643901 |
| indirect immunofluorescence test and enzyme-linked immunosorbent assay for detection of campylobacter pylori. | an indirect immunofluorescence test (iif) has been developed for detecting campylobacter pylori in gastroduodenal biopsies. this test was compared with standard methods of c. pylori diagnosis, namely gram staining and urease test, in a study population of 226 patients; 121 of the biopsy specimens were cultured for c. pylori as well. c. pylori colonization was detected in 154 of 226 patients (68%) by at least one of these methods (iif, 96%; gram staining, 78%; urease test, 60%; cultivation, 55%). ... | 1989 | 2644295 |
| genetics of pertussis toxin. | pertussis toxin (pt) is the major virulence factor of bordetella pertussis. the cloning and nucleotide sequencing of the pt genes from b. pertussis, bordetella parapertussis and bordetella bronchiseptica has elucidated the evolution of the bordetella species and allowed considerable advances towards the understanding of their gene expression and the development of safer vaccines against pertussis. | 1989 | 2654538 |
| [catalytic component of calmodulin-independent adenylate cyclase from bovine brain. isolation and determination of partial amino acid sequence]. | the catalytic component of calmodulin-independent adenylate cyclase of cattle cerebral cortex was solubilized and purified to the homogeneous state. the conditions for preparative obtaining of the enzyme on the column with immobilized antibodies to adenylate cyclase were found. these antibodies were proved to interact with the calmodulin-independent rather than the calmodulin-dependent form of the enzyme. molecular mass of the calmodulin-independent adenylate cyclase determined electrophoretical ... | 1989 | 2662976 |
| effect of a phospho-oligosaccharidic putative insulin messenger on insulin release in rats. | the phospho-oligosaccharide extracted from rat liver and supposed to act as the insulin second messenger inhibits glucose-stimulated insulin release. in the present study, this phospho-oligosaccharide was found not to affect d-[u-14c]glucose oxidation and 45ca net uptake, but to inhibit insulin release evoked by either d-glucose or 2-ketoisocaproate in isolated rat islets. the relative extent of the latter inhibition was unaffected by either the concentration of d-glucose or the presence of dibu ... | 1989 | 2666215 |
| in vitro and in vivo activities of qa-241, a new tricyclic quinolone derivative. | the in vitro susceptibilities of 1,310 clinical isolates to qa-241, a novel tricyclic quinolone, were evaluated in comparison with susceptibilities to norfloxacin, ofloxacin, enoxacin, and ciprofloxacin. the mics of qa-241 for 90% of staphylococci, enterococcus faecalis isolates, and streptococcal species ranged from 1.56 to 6.25 micrograms/ml, and the activity of qa-241 was similar to those of norfloxacin and enoxacin but two to four times less potent than those of ofloxacin and ciprofloxacin. ... | 1989 | 2679369 |
| [pharmacokinetic and clinical studies on cefpodoxime proxetil dry syrup in the field of pediatrics]. | cefpodoxime proxetil (cpdx-pr, cs-807) is a new oral cephem derivative drug in which carboxylic acid was esterified to the 4-position of cpdx (oxime type cephem antibiotic). cpdx-pr is hydrolyzed mainly with esterase in intestinal wall and cpdx exists as an active form in body fluid. while there are numerous study reports using cpdx-pr in tablet forms in japan, the dry syrup formula for pediatric use was newly developed. the dry syrup of cpdx-pr was orally administered 20 minutes after meal to t ... | 1989 | 2681864 |
| mutants of pertussis toxin suitable for vaccine development. | immunization with chemically detoxified pertussis toxin can prevent severe whooping cough with an efficacy similar to that of the cellular pertussis vaccine, which normally gives unwanted side effects. to avoid the reversion to toxicity and the loss of immunogenicity that may follow chemical treatment of pertussis toxin, inactive toxins were constructed by genetic manipulation. a number of genetically engineered alleles of the pertussis toxin genes, constructed by replacing either one or two key ... | 1989 | 2683073 |
| induction of interstitial nephritis in rats by basement membrane of human origin. | a study was conducted to investigate nephritogenic tubular basement membrane antigens common to human and rat kidneys. brown norway (bn) rats were immunized with human renal basement membrane in complete freund's adjuvant simultaneously with bordetella pertussis vaccine. the immunized rats developed polyuria and increased levels of serum creatinine one week after the second immunization. renal histology at this time revealed marked, acute tubulointerstitial nephritis with linear deposition of ig ... | 1989 | 2688356 |
| prevalence of pertussis igg antibodies in children in palermo, italy. | the prevalence of igg antibodies to bordetella pertussis in a sample of 615 1-12-year-old unvaccinated children in palermo was estimated by elisa. the overall prevalence was 56%; it increased from 24% in one to three-year-old children to 67% in 11-12-year-old children (p less than 0.01). igg antibody prevalence was not associated with the father's years of schooling (or 1), nor with the family size (or 1.3; c.i. 95% = 0.8-2.2). for children aged one the three years, serological results showed th ... | 1989 | 2689344 |
| expression of pertussis toxin in bordetella bronchiseptica and bordetella parapertussis carrying recombinant plasmids. | pertussis toxin is produced only by strains of bordetella pertussis. cloned genes encoding pertussis toxin from b. pertussis were transferred into bordetella bronchiseptica and bordetella parapertussis by conjugation. these transconjugants expressed pertussis toxin at levels comparable to those expressed by b. pertussis. the toxin made by these strains was biologically active in the chinese hamster cell clumping assay, contained all five subunits, and was mostly periplasmic. toxin expression app ... | 1989 | 2707851 |
| calmodulin-independent bovine brain adenylate cyclase. amino acid sequence and nucleotide sequence of the corresponding cdna. | an individual catalytic component of calmodulin-independent adenylate cyclase has been isolated from bovine brain cortex. affinity chromatography on an immunosorbent was used. the amino acid sequence of adenylate cyclase as well as the corresponding nucleotide sequence of the cdna has been determined. cdna of adenylate cyclase encodes a protein consisting of 834 amino acid residues and the signal peptide (19 amino acid residues). a series of adenylate cyclase isoforms has been found. a homology ... | 1989 | 2776887 |
| in vitro induction of antigen specific antibody synthesis and proliferation of t lymphocytes with acellular pertussis vaccines, pertussis toxin and filamentous haemagglutinin in humans. | the in vitro response of human b- and t-lymphocytes to the acellular vaccines jnih-6 (containing pertussis toxoid and filamentous hemagglutinin), and jnih-7 (containing pertussis toxoid), and to the purified components jnih-4 (filamentous hemagglutinin) and jnih-5 (pertussis toxin) was investigated. pertussis toxoid and filamentous hemagglutinin induced specific ig synthesis in vitro in lymphocytes obtained from convalescent pertussis patients as target cells. the antigen-dependent ig production ... | 1989 | 2785517 |
| [the effect of histamine on the rosette-forming ability of t-lymphocytes upon immunization with dpt vaccine and its components]. | the influence exerted by histamine on the capacity of t-lymphocytes for spontaneous rosette formation in intact animals and in animals immunized with adsorbed dpt vaccine and its components has been studied. histamine at a concentration of 10(-3) m has been found to produce an inhibitory effect on the capacity of lymphocytes in the blood and spleen of guinea pigs immunized with adsorbed dpt vaccine and its components for spontaneous rosette formation. this effect of histamine has proved to be ev ... | 1989 | 2786302 |
| sequential study of the histopathology and cellular and humoral immune response during the development of an autoimmune orchitis in wistar rats. | wistar rats immunized with an homologous testicular homogenate (th) and complete freund's adjuvant, followed by i.v. injection of bordetella pertussis, developed an autoimmune orchitis (eao). animals were studied at 7, 16, 30, 50, and 80 days (d) after the first immunization. an important lesion of the testis only appeared at 50 d, increasing in severity and incidence (77%) at 80 d. lesions were characterized by a prevalent aspermatogenesis with tubular atrophy and mild interstitial mononuclear ... | 1989 | 2803528 |
| photolabeling of glu-129 of the s-1 subunit of pertussis toxin with nad. | uv irradiation was shown to induce efficient transfer of radiolabel from nicotinamide-labeled nad to a recombinant protein (c180 peptide) containing the catalytic region of the s-1 subunit of pertussis toxin. incorporation of label from [3h-nicotinamide]nad was efficient (0.5 to 0.6 mol/mol of protein) relative to incorporation from [32p-adenylate]nad (0.2 mol/mol of protein). label from [3h-nicotinamide]nad was specifically associated with glu-129. replacement of glu-129 with glycine or asparti ... | 1989 | 2807535 |
| enhancing effect of suspended particulate matter on the ige antibody production in mice. | suspended particulate matter (spm), suspended in the polluted environmental atmosphere, are perpetually inhaled into the human body and are considered to have profound effects on human health. this study investigated the enhancing effect of spm on the ige antibody production in mice. the ige antibody responses in mice immunized with intranasal administration of ovalbumin (oa) plus spm at 3-week intervals were higher than responses in the animals immunized with oa alone. when the dose of oa admin ... | 1989 | 2807574 |
| development of a rapid diagnostic test for pertussis: direct detection of pertussis toxin in respiratory secretions. | monoclonal antibodies (mab) were produced against the specific bordetella pertussis antigen pertussis toxin (pt). in preliminary studies, one mab (ib12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of pt in control experiments and to test its potential for direct detection of pt in nasopharyngeal secretion (np) specimens from patients with confirmed cases of whooping cough. the dot blot assay was able to detect pt at ... | 1989 | 2808670 |
| [pharmacokinetic and clinical studies of cefteram pivoxil granule in the pediatric field]. | cefteram pivoxil (cftm-pi), the pivaloyloxymethyl ester of cefteram (cftm) in which aminothiazol was also introduced into the 7 position of cephem nucleus, is a new oral cephem antibiotic. cftm-pi was absorbed through the intestines and hydrolyzed to cftm by esterases in the intestinal wall and existed in the body fluids as cftm. a tablet form of this drug has been released in japan and now a granular form for pediatric patients has been developed. we have determined mics of 5 drugs (cftm, cepha ... | 1989 | 2810762 |
| gene isolation by direct in situ camp binding. | the regulatory subunit of the camp-dependent protein kinase expressed in clones isolated by immunoscreening of a lambda gt11 cdna library from dictyostelium discoideum exhibits high affinity for camp [mutzel et al., proc. natl. acad. sci. usa 84 (1987) 6-10]. based on this property, we have developed a screening procedure to detect in situ camp-binding activity directly on phage plaques transferred to nitrocellulose filters. highly radioactive camp was synthesized using [alpha-32p]atp at 3000 ci ... | 1987 | 2826298 |
| multiple receptors linked to inhibition of adenylate cyclase accelerate na+/h+ exchange in neuroblastoma x glioma cells via a mechanism other than decreased camp accumulation. | alpha 2-adrenergic receptors, a population of receptors linked to inhibition of adenylate cyclase, accelerate na+/h+ exchange in ng108-15 neuroblastoma x glioma cells (isom, l. l., cragoe, e. j., jr., and limbird, l. e. (1987) j. biol. chem. 262, 6750-6757). we now report that two other receptor populations linked to inhibition of adenylate cyclase, muscarinic cholinergic and delta-opiate receptors, also alkalinize the interior of ng108-15 cells, as measured with the ph-sensitive fluorescent pro ... | 1987 | 2826423 |
| pharmacology of 5-hydroxytryptamine-1a receptors which inhibit camp production in hippocampal and cortical neurons in primary culture. | serotonin (5-hydroxytryptamine, 5-ht) inhibited the formation of camp promoted by vasoactive intestinal polypeptide, plus forskolin, in mouse hippocampal and cortical neurons in primary culture. the rank order of potencies of classical 5-ht1 agonists in inhibiting camp formation in hippocampal neurons was 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) greater than 5-carboxamidotryptamine (5-ct) greater than d-lysergic acid diethylamide greater than 5-ht greater than 5-methoxy-n,n-dimethyltry ... | 1988 | 2828913 |
| cardiovascular response to beta-adrenergic and muscarinic cholinergic receptor stimulation deteriorates in bordetella pertussis-vaccinated pigs. | | 1988 | 2833082 |
| activation of adenylate cyclase in rat fat cells promotes an increase in gtp content which controls the enzyme activity. | in previous studies, we demonstrated that the treatment of adipocytes with cholera toxin or bordetella pertussis toxin (iap) promoted an increase in the total guanosine triphosphate (gtp) content of the cells concomitant with the increase in cyclic adenosine monophosphate (amp) level and the resulting lipolysis. in the present studies, we show that the acute challenge of fat cells with 1 microm isoproterenol (ipne) is associated with a transient increase in gtp level (3-fold in 6 min). this incr ... | 1988 | 2833416 |
| endotoxin-associated protein: a potent stimulus for human granulocytopoietic activity which may be accessory cell independent. | proteins coextracted with endotoxin, termed endotoxin-associated protein (eap), have been shown to exert interleukin 1-like activities. the present studies demonstrate that eap also exerts potent granulopoietic colony-stimulating activity (csa) on human peripheral blood and bone marrow progenitor cells, comparable to that seen with various types of conditioned media. the csa observed with eap appeared to be heat (100 degrees c, 30 min) and trypsin resistant and partially pronase resistant. simil ... | 1988 | 2836311 |
| second messengers associated with the action of aii and dopamine d2 receptors in anterior pituitary. relationship with prolactin secretion. | we have shown that da receptors of the d2 subtype inhibit prolactin release by several mechanisms. da receptors inhibit cyclic amp production through a gtp binding protein sensitive to the bordetella pertussis toxin. however, this mechanism cannot be involved in the blockade of the aii stimulated prolactin secretion by da. this blockade is probably partly due to the inhibition of the aii-stimulated inositol phosphate production by da. this inhibition is also sensitive to the bordetella pertussis ... | 1988 | 2838617 |
| mechanism of neutrophil activation by naf, a novel monocyte-derived peptide agonist. | the rise in cytosolic free ca2+, shape change, superoxide formation, and granule exocytosis induced in human neutrophils by n-formyl-met-leu-phe (fmlp) and by a newly discovered activating peptide, neutrophil-activating factor, termed naf, were compared. naf was effective in the concentration range of 0.1-10 nm and was 10- to 100-fold more potent than fmlp. in qualitative terms, the single responses to either stimulus were remarkably similar: they showed virtually identical onset and initial kin ... | 1988 | 2840318 |
| cloning and expression of the calmodulin-sensitive bacillus anthracis adenylate cyclase in escherichia coli. | the adenylate cyclase gene of bacillus anthracis, encoding the edema factor, a component of anthrax toxin, has been cloned and expressed in escherichia coli. clones were selected by their capacity to complement the cyclase deficiency (cya-) of an e. coli strain expressing the eukaryotic protein calmodulin, an essential activator of b. anthracis adenylate cyclase. the protein expressed in e. coli was shown to exhibit adenylate cyclase activity only in the presence of calmodulin. experiments using ... | 1988 | 2841199 |
| actions of cyclic adenosine monophosphate on the cytodifferentiation of ovarian cells: studies in cultured swine granulosa cells using a novel exogenous adenylate cyclase from bordetella pertussis. | the regulation by camp of cholesterol side-chain cleavage activity and the synthesis of immunoisolated cytochrome p-450scc and adrenodoxin proteins was investigated in primary cultures of swine ovarian (granulosa) cells. administration of a novel adenylate cyclase toxin isolated from bordetella pertussis increased granulosa-cell camp accumulation up to 200-fold over basal. these effects were additive with those of fsh, forskolin, and cholera toxin. in contrast, bacterial extracts bp 347 and bp 3 ... | 1988 | 2843759 |
| interactions of protein kinase c with receptor- and non-receptor-mediated cyclic amp generation in swine granulosa cells. | interactions between signal transducing systems may be important in the integrated control of cellular processes in basal and hormonally regulated cells. the swine granulosa cell provides a model to study the interactions between the camp and calcium-lipid-dependent signaling pathways. to this end, porcine granulosa cells were incubated in monolayer culture for 1-4 days in the presence of fsh (200 ng/ml), forskolin (85 microm), or cholera toxin (3 micrograms/ml) with or without an activator of p ... | 1988 | 2846382 |
| leukotriene d4 receptor-mediated phosphoinositol hydrolysis and calcium mobilization in rat basophilic leukemic cells. | a basophilic leukemic cell line from rat (rbl-1) was used to characterize leukotriene d4 (ltd4) receptor-mediated biochemical and pharmacological effects. [3h]ltd4 binding to the plasma membrane enriched preparation was stereo-selective, specific and saturable. sodium ions and guanine nucleotides specifically regulated [3h]ltd4 binding to the membrane receptors. leukotriene e4 (lte4) and high affinity specific antagonists bound to the receptor with a rank-order potency equivalent to that for the ... | 1988 | 2846829 |
| [polymyxin b suppresses the stimulating action of pertussis vaccine on hematopoiesis in mice]. | the injection of killed whole-cell pertussis vaccine (pv), prepared from formulated bordetella pertussis strains 5574 and 305, into mice was shown to produce a stimulating effect on hematopoiesis. this effect was manifested by an increase in the number of endogenic colonies developing in the spleen of mice on days 5 and 9 after their irradiation in a sublethal dose, by the sharp stimulation of the proliferative activity of splenic colony-forming units (cfus) originating in the bone marrow and by ... | 1988 | 2851246 |
| suppression of leukotriene synthesis in human leukocytes by a urea extract of bordetella pertussis: evidence for mediation by adenylate cyclase toxin. | incubation of human leukocytes with a urea extract of bordetella pertussis led to inhibition of zymosan-induced leukotriene generation. the proteins in this extract are known to include an adenylate cyclase which suppresses certain defense mechanisms of leukocytes against bacterial invasion. the formation of leukotriene b4 and leukotriene c4, induced by serum-coated zymosan, was almost completely inhibited in the presence of 75 micrograms of urea-extracted proteins/ml cell suspension. this suppr ... | 1988 | 2853462 |
| outer membrane permeability in pseudomonas cepacia: diminished porin content in a beta-lactam-resistant mutant and in resistant cystic fibrosis isolates. | since beta-lactam resistance is a feature of pseudomonas cepacia isolates causing pulmonary infections in cystic fibrosis (cf), this study was undertaken to determine whether alterations in beta-lactam permeability mediate drug resistance in this species. a beta-lactam-susceptible non-cf isolate (strain 75-26), a resistant mutant derived from 75-26 by selection for cross-resistance to ciprofloxacin and ceftazidime, and two resistant cf isolates of p. cepacia were used. permeability constants wer ... | 1988 | 2855296 |
| biochemical properties of pertussis toxin. | pertussis toxin is an exotoxin produced by the organism bordetella pertussis. the toxin binds to receptors on the eukaryotic cell surface. after introduction into the eukaryotic cell, the toxin is activated by atp and subsequently adp-ribosylates a family of gtp-binding regulatory proteins interrupting signal transduction within the cell. we have examined the location of several critical sites on the toxin molecule. these sites include the receptor binding site and the atp binding site. the b ol ... | 1988 | 2856219 |
| antibodies to bordetella pertussis in human colostrum and their protective activity against aerosol infection of mice. | colostrum samples from indonesian mothers were assayed for antibodies which agglutinate bordetella pertussis and for antibodies to the filamentous hemagglutinin and the lymphocytosis-promoting factor of b. pertussis. agglutinins were assayed by a microtiter method, and 36 of 58 samples tested (62%) had titers above 1:10 (range, less than 1:10 to 1:160). an enzyme-linked immunosorbent assay detected anti-filamentous hemagglutinin in 39 of 60 samples (65%) and anti-lymphocytosis-promoting factor i ... | 1985 | 2857154 |
| an assay of bordetella pertussis adhesion to tissue-culture cells. | the ability of bordetella pertussis to bind to cell surfaces was determined with a simple, accurate, reproducible assay measuring the adhesion of radiolabelled bacteria to monolayers of hela cells. the rate of adhesion was approximately linear with time for at least 1 h. viable and radioactivity counts of bound bacteria correlated well. bacteria grown in the avirulent c-mode were markedly less adhesive than virulent x-mode cells. reductions in the level of attachment after treatment of bacteria ... | 1985 | 2857202 |
| specific binding of endotoxin to human monocytes and mouse macrophages: serum requirement. | specific binding of bordetella pertussis and neisseria meningitidis endotoxins to human monocytes and murine macrophages was demonstrated. binding of b. pertussis endotoxin could be inhibited by endotoxins of salmonella minnesota, escherichia coli, and klebsiella pneumoniae, the extent of inhibition being dependent on the origin of the lipopolysaccharides and on the origin of the mononuclear phagocytic cells. the binding of b. pertussis and n. meningitidis endotoxins which was mediated by the po ... | 1985 | 2857597 |
| neutralizing antibodies to pertussis toxin in whooping cough. | the development and duration of neutralizing antibodies (antitoxin) to pertussis toxin were studied in 38 patients with culture-verified infections due to bordetella pertussis and one patient with infection due to bordetella parapertussis. an in vitro neutralization test in microplate culture of chinese hamster ovary cells was used. an antitoxin response was recorded in 36 patients, the exceptions being two patients treated early with erythromycin (one of whom developed clinical pertussis two ye ... | 1985 | 2857752 |
| purification and characterization of fimbriae isolated from bordetella pertussis. | fimbriae were detached from bordetella pertussis by mechanical shearing and purified by successive precipitations with ammonium sulfate, phosphate buffer (ph 6.0), and magnesium chloride. in each of these purification steps, the fimbriae aggregated into bundles as seen by electron microscopy. these aggregates could be disaggregated at ph 9.5. by electron microscopy, the purified fimbriae appeared as long filaments with a diameter of 5 nm. sodium dodecyl sulfate-polyacrylamide gel electrophoresis ... | 1985 | 2859248 |
| bordetella pertussis invasive adenylate cyclase. partial resolution and properties of its cellular penetration. | the existence of an invasive adenylate cyclase in dialyzed urea extracts of the bacterium bordetella pertussis has been suggested recently. gel filtration of b. pertussis dialyzed urea extract shows that the invasive enzyme constitutes only a small portion of the total adenylate cyclase activity found in the extract. its size is different than the size of the two peaks of adenylate cyclase activity identified in the extract. ca2+ is absolutely required for the penetration of the invasive enzyme, ... | 1985 | 2859287 |
| bordetella pertussis: growth in stainless steel. | | 1985 | 2859289 |
| the effect of growth conditions on adenylate cyclase activity and virulence-related properties of bordetella pertussis. | growth of bordetella pertussis in stainer & scholte medium in which the nacl had been replaced by one of several inorganic or organic salts resulted in a large decrease in adenylate cyclase activity, histamine-sensitizing activity and in the amounts of two cell-envelope polypeptides of mr 28000 and 30000. although some variation between strains was observed, there was never a case where one of these properties was lost independently of the others. cultures in which these properties were lost had ... | 1985 | 2859346 |
| adenylate cyclase activity during phenotypic variation of bordetella pertussis. | during mgso4-induced modulation of bordetella pertussis, adenylate cyclase activity, histamine-sensitizing activity (hsa) and the major cell-envelope polypeptides with mr 28000 and 30000 (x polypeptides) were lost synchronously at a rate which could be accounted for by a simple growth-dilution effect. mgso4 and other compounds which induced the above phenotypic change caused little inhibition of adenylate cyclase activity. nicotinic acid was the sole exception and at 4.1 mm-caused 60% inhibition ... | 1985 | 2859347 |
| [cell motility in a system of mononuclear phagocytes from different strains of mice in the presence of bacteria and their components]. | the motility of organospecific macrophages of the abdominal cavity, spleen, lungs and liver, observed in the presence of serogroup a neisseria meningitidis polysaccharide, formalin-killed bordetella pertussis and acetone-treated salmonella typhimurium, has been studied. in these experiments cba, c57bl/6 and (cba x c57bl/6)f1 mice have been used. the in vitro study of the motility of cells in the system of mononuclear phagocytes in the presence of bacterial antigens has revealed that these cells ... | 1985 | 2859722 |
| [characteristics of the immune response to a single peroral administration of different doses of corpuscular pertussis vaccine]. | the effect of oral administrations of different doses of pertussis vaccine on the humoral and cell-mediated responses of systemic immunity and on the immunomorphological transformation of the mucous membrane of the small intestine was studied in (cba x c57bl/6)f1 mice. on day 28 after the administration of all the tested doses of pertussis vaccine the animals were found to have a high degree of protection from the development of meningoencephalitis induced by the inoculation of bordetella pertus ... | 1985 | 2859723 |
| [pathogenetic significance of bordetella pertussis toxin]. | | 1985 | 2860059 |
| stainer and scholte's pertussis medium with an alternative buffer. | a comparative study of the stainer and scholte chemically defined growth medium (ss) for bordetella pertussis, and a modification of it (mss), was made. the tris buffer which is reported to have adverse effects was replaced in mss with sodium glycerophosphate. by using generation time as a measure of the growth promoting properties of the media, we found that the mss was superior to ss for b. pertussis strain cn5612/67, which was used as a vaccine strain in norway. additionally by employing the ... | 1985 | 2860114 |
| antibody response of infants to haemophilus influenzae type b capsular polysaccharide combined with bordetella pertussis. | | 1985 | 2860187 |
| pertussis vaccine: present status and future prospects. | use of killed whole-cell bordetella pertussis vaccines has been a major factor in control of symptomatic whooping cough (pertussis). in the uk, diminished public confidence in the safety of this vaccine led to a reduction in vaccine acceptance which correlated with an increase in the incidence of pertussis. there is a need for acellular pertussis vaccines of low toxicity which, ideally, will prevent colonization and also protect against the disease symptoms. vaccine design can rely increasingly ... | 1985 | 2860757 |
| mixed outbreak of bordetella pertussis and bordetella parapertussis infection in finland. | the epidemiology of whooping cough in a vaccinated population was studied during an outbreak of paroxysmal cough in an elementary school with 258 pupils in turku, finland. nasopharyngeal specimens for isolation of bordetella pertussis and/or sera for elisa detection of antipertussis immunoglobulin m, a and g antibodies were taken from 94% of children who were prospectively followed for two months. bordetella pertussis was isolated in six patients, and 17 culture-positive cases with bordetella pa ... | 1985 | 2861090 |
| the role of serum in interleukin 1 production by human monocytes activated by endotoxins and their polysaccharide moieties. | lipopolysaccharides (lps) as well as polysaccharide (ps) moieties of bordetella pertussis and neisseria meningitidis endotoxins induced in vitro interleukin 1 (il 1) secretion by human monocytes as evaluated by the co-mitogenic assay on c3h/hej thymocytes. because of the role of serum in the specific binding of endotoxins to monocytes mediated by the polysaccharide region [12], experiments were undertaken to study the effect of serum on il 1 induction. although the presence of serum is not neces ... | 1985 | 2861158 |
| susceptibility of bordetella pertussis to doxycycline, cinoxacin, nalidixic acid, norfloxacin, imipenem, mecillinam and rifampicin. | the susceptibility of bordetella pertussis to doxycycline, cinoxacin, nalidixic acid, norfloxacin, imipenem (n-formimidoyl-thienamycin), mecillinam and rifampicin was studied by agar and broth dilution. there were discrepancies between mics registered on solid medium and in fluid medium. there seems to be a need for methodological studies on the antibiotic susceptibility of bord. pertussis using different techniques. none of the compounds tested seemed to be realistic alternatives to the well do ... | 1985 | 2861190 |
| characterization of two adhesins of bordetella pertussis for human ciliated respiratory-epithelial cells. | bordetella pertussis, the causative agent of whooping cough, adheres specifically to human ciliated respiratory-epithelial cells. in order to identify the adhesins of bordetella, we tested mutant strains, each deficient in one of several bacterial virulence factors, for the ability to adhere to single, human ciliated cells in vitro. loss of adherence was associated with lack of secretion of either of two surface antigens: filamentous hemagglutinin or pertussis toxin. these strains regained adher ... | 1985 | 2861240 |
| effect of prednisolone on the toxicity of bordetella pertussis for mice. | prednisolone, given orally or intraperitoneally before challenge, protected mice against the lethal effect of a crude cell extract of bordetella pertussis containing heat-labile toxin (hlt) as the major toxic component. prednisolone did not diminish the lethal toxicity of heated b. pertussis cell suspensions containing pertussis toxin and endotoxin but devoid of hlt. this suggests that the protective effect of the steroid was directed against the hlt. when live bacteria were injected intraperito ... | 1985 | 2861293 |
| a whole-organ perfusion model of bordetella pertussis adherence to mouse tracheal epithelium. | a whole-organ perfusion system was used to culture tracheas from adult swiss mice and test this system's adaptability for use in adherence assays for virulent bordetella pertussis. culture medium and bacterial suspensions flowed readily through the tracheal lumen, ciliary activity was maintained throughout the culture period, and scanning electron microscopy revealed retention of normal surface morphology. the number of adherent colony-forming units (cfu) per trachea was determined for all three ... | 1985 | 2862133 |
| selective regulation of chemotactic lymphokine production. i. selective potentiation of eosinophil chemotactic lymphokine production in alum hydroxy gel- and bordetella pertussis vaccine-treated guinea pigs. | delayed tissue eosinophilia in dnp-ovalbumin-induced allergic inflammatory skin lesions of guinea pigs was markedly enhanced by previous treatment with alum hydroxy gel (alum) or bordetella pertussis vaccine. this enhancement seemed due to increased production of a lymphocyte-derived eosinophil chemotactic factor (ecf) at the skin site. treatment of animals with alum potentiated antigen-induced in vitro ecf production by lymphoid cells from spleen and mesenteric lymph node of sensitized animals. ... | 1985 | 2864377 |
| genetic analysis of bacterial virulence determinants in bordetella pertussis and the pathogenetic yersinia. | | 1985 | 2865085 |
| one-dimensional peptide mapping of the subunits of pertussis toxin. | pertussis toxin (pertussigen) purified from the cytoplasmic fraction of bordetella pertussis strain 18334, phase 1, consisted of five subunits which included an additional subunit (s1a) not previously reported. subunits s1, s1a and s2 showed extensive structural homology when analysed by one-dimensional peptide mapping, indicating that the latter two were probably derived from proteolytic cleavage of the largest subunit, s1. subunits s3 and s4,5 generated only a limited number of peptides follow ... | 1985 | 2865327 |
| [cryofractographic study of intercellular junctions in the populations of agar-cultivated bordetella pertussis]. | the characteristic feature of replicas obtained from the freeze-fractures of b. pertussis unfixed cultures developing on casein charcoal agar for 1-7 days is the associative growth of highly polymorphic cells, ensured by the ramified system of intercellular connections (ic) formed by the derivatives of the outer layers of the cell wall. this proves that the associative location of bacterial cells, linked by numerous ic, in the preparation is not the artefact appearing in the process of their che ... | 1985 | 2866645 |
| [obtaining monoclonal antibodies to bordetella pertussis antigens]. | the immunization of mice with the protein fraction of b. pertussis strain 305 has made it possible to obtain hybridomas producing monoclonal antibodies to b. pertussis antigens. ascitic fluids containing monoclonal antibodies react in the elisa in high titers and actively agglutinate b. pertussis strains 305 and 475. | 1985 | 2866646 |