| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| unusual methylobacterium fujisawaense infection in a patient with acute leukaemia undergoing hematopoietic stem cell transplantation: first case report. | microorganisms of the genus methylobacterium are facultative methylotrophic, gram-negative rods that are ubiquitous in nature and rarely cause human disease, mostly in subjects with preexisting causes of immune depression. methylobacterium fujisawaense, first proposed as a new species in 1988, has never been reported as a bacterial agent of human infections so far. here we describe a case of m. fujisawaense infection in a relapsed acute leukaemia undergoing unrelated allogeneic hematopoietic ste ... | 2010 | 20396386 |
| adhesion and biofilm formation on polystyrene by drinking water-isolated bacteria. | this study was performed in order to characterize the relationship between adhesion and biofilm formation abilities of drinking water-isolated bacteria (acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staphylococcus sp.). adhesion was assessed by two distinct methods: thermodynamic prediction of adhesion potential by quantifying hydrophobicity and the free energy of adhesion; and by microtiter plate assays. biofilms w ... | 2010 | 20405208 |
| [key functional genes of aerobic methylolbacteria capable of degrading dichloromethane]. | | 2010 | 20411663 |
| identification of a methylase required for 2-methylhopanoid production and implications for the interpretation of sedimentary hopanes. | the rise of atmospheric oxygen has driven environmental change and biological evolution throughout much of earth's history and was enabled by the evolution of oxygenic photosynthesis in the cyanobacteria. dating this metabolic innovation using inorganic proxies from sedimentary rocks has been difficult and one important approach has been to study the distributions of fossil lipids, such as steranes and 2-methylhopanes, as biomarkers for this process. 2-methylhopanes arise from degradation of 2-m ... | 2010 | 20421508 |
| organising metabolic networks: cycles in flux distributions. | metabolic networks are among the most widely studied biological systems. the topology and interconnections of metabolic reactions have been well described for many species. this is, however, not sufficient to understand how their activity is regulated in living organisms. these descriptions depict a static set of possible chains of reactions, with no information about the dynamic activity of reaction fluxes. cyclic structures are thought to play a central role in the homeostasis of biological sy ... | 2010 | 20435049 |
| functional investigation of methanol dehydrogenase-like protein xoxf in methylobacterium extorquens am1. | methanol dehydrogenase-like protein xoxf of methylobacterium extorquens am1 exhibits a sequence identity of 50 % to the catalytic subunit mxaf of periplasmic methanol dehydrogenase in the same organism. the latter has been characterized in detail, identified as a pyrroloquinoline quinone (pqq)-dependent protein, and shown to be essential for growth in the presence of methanol in this methylotrophic model bacterium. in contrast, the function of xoxf in m. extorquens am1 has not yet been elucidate ... | 2010 | 20447995 |
| [distribution of bacteria of methylobacterium genus in the terrestrial biotopes of the antarctic region]. | methylotrophs distribution has been studied in the terrestrial biotopes (moss, lichen, grass, soil, sludge of lakes) on the islands of galindez, barkhans, irizar, uruguay, jalour, petermann, berthelot, cruls, king george, corner, skua located in the pacific sector of the antarctic region, as well as in analogous biotopes on the western shore of the antarctic peninsula basing on a complex of diagnosis features the isolated pink-pigmented strains, which facultatively use methanol and realize the s ... | 2009 | 20455426 |
| [microbiological analysis of terrestrial biotopes of the antarctic region]. | microbiological analysis has been made of 120 samples from biotopes of the western coast of the antarctic peninsula (rasmussen cope, tuxen cope, waugh mountain), argentine archipelago islands (galindez, skua, corner, barchans, irizar, uruguay, cluls, three little pigs, king-george), as well as neighbouring islands (petermann--on the north, a group of jalour islands--on the east, berthelot--on the south-east); and more remote islands (darboux, lippmann, booth). it was found out that the total num ... | 2010 | 20455435 |
| enrichment and cultivation of pelagic bacteria from a humic lake using phenol and humic matter additions. | individual bacterial populations are known to respond differently toward substrate availability. to test how the availability of either pure phenol or natural humic matter (hm) selects for specific pelagic bacteria phylotypes from a humic lake (lake grosse fuchskuhle, northeastern germany), we used culture-dependent and -independent approaches. using a batch approach, the bacterial community composition (bcc) differed depending on both the quantity and the quality of added substrates. using a di ... | 2010 | 20459514 |
| methylobacterium marchantiae sp. nov., a pink-pigmented, facultatively methylotrophic bacterium isolated from the thallus of a liverwort. | a pink-pigmented, facultatively methylotrophic bacterium, designated strain jt1(t), was isolated from a thallus of the liverwort marchantia polymorpha l. and was analysed by using a polyphasic approach. comparative 16s rrna gene sequence analysis placed the strain in a clade with methylobacterium adhaesivum ar27(t), methylobacterium fujisawaense dsm 5686(t), methylobacterium radiotolerans jcm 2831(t) and methylobacterium jeotgali s2r03-9(t), with which it showed sequence similarities of 97.8, 97 ... | 2011 | 20495043 |
| colonization of rice and spodoptera frugiperda j.e. smith (lepidoptera: noctuidae) larvae by genetically modified endophytic methylobacterium mesophilicum. | the colonization of spodoptera frugiperda j.e. smith larvae and rice seedlings by genetically modified endophytic bacterium methylobacterium mesophilicum, and also the possible transfer of this bacterium to inside the larva's body during seedlings consumption were studied. the data obtained by bacterial reisolation and fluorescence microscopy showed that the bacterium colonized the rice seedlings, the larva's body and that the endophytic bacteria present in seedlings could be acquired by the lar ... | 2010 | 20498973 |
| a new extreme environment for aerobic anoxygenic phototrophs: biological soil crusts. | biological soil crusts improve the health of arid or semiarid soils by enhancing water content, nutrient relations and mechanical stability, facilitated largely by phototrophic microorganisms. until recently, only oxygenic phototrophs were known from soil crusts. a recent study has demonstrated the presence of aerobic representatives of earth's second major photosynthetic clade, the evolutionarily basal anoxygenic phototrophs. three canadian soil crust communities yielded pink and orange aerobic ... | 2010 | 20532732 |
| production of the chiral compound (r)-3-hydroxybutyrate by a genetically engineered methylotrophic bacterium. | in this study, a methylotrophic bacterium, methylobacterium rhodesianum mb 126, was used for the production of the chiral compound (r)-3-hydroxybutyrate (r-3hb) from methanol. r-3hb is formed during intracellular degradation of the storage polymer (r)-3-polyhydroxybutyrate (phb). since the monomer r-3hb does not accumulate under natural conditions, m. rhodesianum was genetically modified. the gene (hbd) encoding the r-3hb-degrading enzyme, r-3hb dehydrogenase, was inactivated in m. rhodesianum. ... | 2010 | 20581197 |
| isolation and characterization of new poly(3hb)-accumulating star-shaped cell-aggregates-forming thermophilic bacteria. | this study aimed at isolating thermophilic bacteria that utilize cheap carbon substrates for the economically feasible production of poly(3-hydroxybutyrate), poly(3hb), at elevated temperatures. | 2010 | 20584099 |
| temporal bacterial diversity and detection of putative methanotrophs in surface mats of lonar crater lake. | the phylogenetic diversity of bacterial communities in microbial mats of two different seasons from saline and hyperalkaline lonar lake was investigated using 16s rrna gene library analysis. arthrospira (cyanobacteria) related clones (>80% of total clones) dominated libraries of both the seasons. clear differences were found in both the seasons as the operational taxonomic units (otus) related to fusibacter (lai-1 and lai-59) and tindallia magadiensis (lai-27) found in post-monsoon were not foun ... | 2010 | 20586073 |
| monitoring the plant epiphyte methylobacterium extorquens dsm 21961 by real-time pcr and its influence on the strawberry flavor. | besides its influence on plant growth and health, plant-associated bacteria exert an impact on fruit quality. methylotrophic bacteria can enhance the biosynthesis of strawberry flavor compounds, especially the two furanoid compounds 2,5-dimethyl-4-hydroxy-2h-furanone (dmhf) and 2,5-dimethyl-4-methoxy-2h-furanone in vitro. here, we report the selection and characterization of methylobacterium extorquens dsm 21961, a strain that was able to enhance the furanone content ad planta under greenhouse c ... | 2010 | 20662926 |
| [resistance to uv radiation of microorganisms isolated from the rock biotopes of the antarctic region]. | microbiological analysis of terrestrial biotopes of the antarctic region has shown, that vertical rocks of the antarctic islands open for the sun were characterized by special microcenoses. the wide distribution of pigmented microorganisms in the rock antarctic samples, a higher frequency of their occurrence, the total number and biologic diversity, than in other antarctic biotopes, has been demonstrated. for the first time the presence of bacteria and yeast, resistant to high doses of uv radiat ... | 2010 | 20695223 |
| competitiveness of diverse methylobacterium strains in the phyllosphere of arabidopsis thaliana and identification of representative models, including m. extorquens pa1. | facultative methylotrophic bacteria of the genus methylobacterium are consistently found in association with plants, particularly in the phyllosphere. to gain a better understanding of the mechanisms underlying the dispersal and occurrence of methylobacterium on plants, diverse strains were isolated, identified, and studied with regard to their competitiveness on the model plant arabidopsis thaliana. as a basis for this study a comprehensive collection of methylobacterium isolates was establishe ... | 2010 | 20700590 |
| production of functionalized polyhydroxyalkanoates by genetically modified methylobacterium extorquens strains. | methylotrophic (methanol-utilizing) bacteria offer great potential as cell factories in the production of numerous products from biomass-derived methanol. bio-methanol is essentially a non-food substrate, an advantage over sugar-utilizing cell factories. low-value products as well as fine chemicals and advanced materials are envisageable from methanol. for example, several methylotrophic bacteria, including methylobacterium extorquens, can produce large quantities of the biodegradable polyester ... | 2010 | 20846434 |
| biotechnological potential of the ethylmalonyl-coa pathway. | the ethylmalonyl-coa pathway is central to the carbon metabolism of many α-proteobacteria, like rhodobacter sphaeroides and methylobacterium extorquens as well as actinomycetes, like streptomyces spp. its function is to convert acetyl-coa, a central carbon intermediate, to other precursor metabolites for cell carbon biosynthesis. in contrast to the glyoxylate cycle--another widely distributed acetyl-coa assimilation strategy--the ethylmalonyl-coa pathway contains many unique coa-ester intermedia ... | 2010 | 20882276 |
| streamlined pentafluorophenylpropyl column liquid chromatography-tandem quadrupole mass spectrometry and global (13)c-labeled internal standards improve performance for quantitative metabolomics in bacteria. | streamlined quantitative metabolomics in central metabolism of bacteria would be greatly facilitated by a high-efficiency liquid chromatography (lc) method in conjunction with accurate quantitation. to achieve this goal, a methodology for lc-tandem quadrupole mass spectrometry (lc-ms/ms) involving a pentafluorophenylpropyl (pfpp) column and culture-derived global (13)c-labeled internal standards (i.ss.) has been developed and compared to hydrophilic interaction liquid chromatography (hilic)-ms/m ... | 2010 | 20950815 |
| methanol assimilation in methylobacterium extorquens am1: demonstration of all enzymes and their regulation. | methylobacterium extorquens am1 is an aerobic facultative methylotrophic α-proteobacterium that can use reduced one-carbon compounds such as methanol, but also multi-carbon substrates like acetate (c₂) or succinate (c₄) as sole carbon and energy source. the organism has gained interest as future biotechnological production platform based on methanol as feedstock. | 2010 | 20957036 |
| microbial quality and nutritional aspects of norwegian brand waters. | the microbiological quality of the five leading brands of norwegian bottled still waters was investigated. all brands were free for the enteric indicator organisms and named pathogens whose absence is demanded in current quality directives. the relatively nutrient-poor agar r₂a revealed large heterogeneous bacterial populations which grew slowly, or not at all, on clinical media specified for use in substrate-utilization approaches to identification. the main approach used for identification was ... | 2010 | 21095035 |
| a systems biology approach uncovers cellular strategies used by methylobacterium extorquens am1 during the switch from multi- to single-carbon growth. | when organisms experience environmental change, how does their metabolic network reset and adapt to the new condition? methylobacterium extorquens is a bacterium capable of growth on both multi- and single-carbon compounds. these different modes of growth utilize dramatically different central metabolic pathways with limited pathway overlap. | 2010 | 21124828 |
| characterization of a protein-generated o₂ binding pocket in pqqc, a cofactorless oxidase catalyzing the final step in pqq production. | pqq is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. the final step of pqq formation is catalyzed by pqqc, a cofactorless oxidase. this study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding and activating pocket in pqqc. the active site variants h154n, y175f,s, and r179s were studied with the goal of defining the site of o(2) binding and activation. using apo-glucose ... | 2011 | 21155540 |
| biofilm-forming bacteria with varying tolerance to peracetic acid from a paper machine. | biofilms cause runnability problems in paper machines and are therefore controlled with biocides. peracetic acid is usually effective in preventing bulky biofilms. this study investigated the microbiological status of a paper machine where low concentrations (≤15 ppm active ingredient) of peracetic acid had been used for several years. the paper machine contained a low amount of biofilms. biofilm-forming bacteria from this environment were isolated and characterized by 16s rrna gene sequencing, ... | 2010 | 21161323 |
| nanoscale ion-pair reversed-phase hplc-ms for sensitive metabolome analysis. | in this article, we introduce a method using nanoscale ion-pair reversed-phase high-performance liquid chromatography (nano-ip-rp-hplc) hyphenated to nanoelectrospray ionization high-resolution mass spectrometry (nano-esi-hrms) to separate and identify metabolites in cell extracts. separation of metabolites was performed on a 100 μm i.d. c18 column with tributylamine (tba) as the ion-pairing reagent and methanol as the eluent. basic ph (9.4) of the mobile phase was critical to achieve sufficient ... | 2010 | 21166460 |
| methylobacterium soli sp. nov. a methanol-utilizing bacterium isolated from the forest soil. | a gram-negative, pink-pigmented, non-spore-forming rod shaped, methanol-utilizing bacterium, strain yim 48816(t), was isolated from forest soil collected from sichuan province, china. strain yim 48816(t) can grow at 4-37 °c, ph 5.0-7.0 and 0% nacl (w/v). based on 16s rrna gene sequence similarity studies, it belonged to the genus methylobacterium, and formed a phyletic line. the 16s rrna gene sequence similarities were 96.2% to methylobacterium mesophilicum dsm 1708(t) and 96.0% to methylobacter ... | 2011 | 21222033 |
| autoregulation of nodulation interferes with impacts of nitrogen fertilization levels on the leaf-associated bacterial community in soybeans. | the diversities leaf-associated bacteria on nonnodulated (nod(-)), wild-type nodulated (nod(+)), and hypernodulated (nod(++)) soybeans were evaluated by clone library analyses of the 16s rrna gene. to analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitrogen (sn) (15 kg n ha(-1)) or heavy nitrogen (hn) (615 kg n ha(-1)) fertilization. under sn fertilization, the relative abundance of alphaproteobacteria was significantly higher in ... | 2011 | 21239540 |
| influence of methylobacterium on iron translocation in plants. | iron metabolism in plants is essential to maintain optimal growth and iron nutrition is dependent on uptake of iron from the environment and movement of iron in the plant tissues. we have examined the translocation of iron in plant leaves following foliar application of feedta to vicia faba and zea mays. using radiolabeled iron, we observed that iron translocation is stimulated by products of methylobacterium mesophylicum and by the cytokinin, kinetin. when cytokinins were applied to leaves alon ... | 2011 | 21240540 |
| molecular structure of la3+-induced methanol dehydrogenase-like protein in methylobacterium radiotolerans. | la(3+) and not ca(2+) increases methanol dehydrogenase (mdh) activity in methylobacterium radiotolerans nbrc15690. la(3+)- and ca(2+)-mdh-like proteins were found to be homodimeric (a(2)) and heterotetrameric (a(2)ß(2)), respectively. n-terminal amino acid sequences of these proteins revealed that la(3+)- and ca(2+)-mdh-like proteins were encoded by xoxf and mxafi, respectively. | 2011 | 21256798 |
| [composite biodegradable materials based on polyhydroxyalkanoate]. | conditions for the processing and mixing of biodegradable polymers at temperatures less than their thermal destruction (130-150 degrees c) using standard equipment have been identified. the structure of the polyhydroxybutyrate/valerate (phb/v) copolymer has been revealed and peculiarities of the crystal phase formation at different monomer ratios have been investigated. it was shown that pure phb with molecular mass 180-270 kda has elastic module approximately 1.2 gpa, strength approximately 25 ... | 2010 | 21261076 |
| cellulase production by pink pigmented facultative methylotrophic strains (ppfms). | pink pigmented facultative methylotrophs (ppfm) isolated from water samples of cooum and adyar rivers in chennai and soil samples of forests located in various districts of tamil nadu, india were screened for cellulase production using carboxymethylcellulose agar (cmc agar) medium. the strains showed wide variations in the production of clearing zones around the colonies on cmc agar medium flooded with congo red. cmcase and filter paper assays were used to quantitatively measure the cellulase ac ... | 2011 | 21274656 |
| silica-immobilized methylobacterium sp. np3 and acinetobacter sp. pk1 degrade high concentrations of phenol. | to immobilize methylobacterium sp. np3 and acinetobacter sp. pk1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. | 2011 | 21291480 |
| molecular analysis of autochthonous microbiota along the digestive tract of juvenile grouper epinephelus coioides following probiotic bacillus pumilus administration. | aims: to evaluate the diversity of dominant autochthonous microbiota along the digestive tract of juvenile epinephelus coioides following the dietary administration of probiotic bacillus pumilus for 60 days. methods and results: polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) with subsequently sequencing analysis was used to assess the gut microbiota. generally similar dgge patterns were observed in the foregut, midgut and hindgut of e. coioides, while the similari ... | 2011 | 21294823 |
| chloride-associated adaptive response in aerobic methylotrophic dichloromethane-utilising bacteria. | aerobic methylotrophic bacteria able to grow with dichloromethane (dcm) as the sole carbon and energy source possess a specific glutathione s-transferase, dcm dehalogenase, which transforms dcm to formaldehyde, used for biomass and energy production, and hydrochloric acid, which is excreted. evidence is presented for chloride-specific responses for three dcm-degrading bacteria, methylobacterium extorquens dm4, methylopila helvetica dm6 and albibacter methylovorans dm10. chloride release into the ... | 2011 | 21298685 |
| cloning and characterization of indolepyruvate decarboxylase from methylobacterium extorquens am1. | for the first time for methylotrophic bacteria an enzyme of phytohormone indole-3-acetic acid (iaa) biosynthesis, indole-3-pyruvate decarboxylase (ec 4.1.1.74), has been found. an open reading frame (orf) was identified in the genome of facultative methylotroph methylobacterium extorquens am1 using blast. this orf encodes thiamine diphosphate-dependent 2-keto acid decarboxylase and has similarity with indole-3-pyruvate decarboxylases, which are key enzymes of iaa biosynthesis. the orf of the gen ... | 2010 | 21314613 |
| facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium. | we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ... | 2011 | 21374057 |
| methylobacterium gossipiicola sp. nov., a pink-pigmented facultative methylotrophic bacteria isolated from cotton phyllosphere. | a pink-pigmented, aerobic, facultatively methylotrophic, gram-negative rod shaped bacterium (gh-105t) isolated from the phyllosphere of cotton, from coimbatore (tamilnadu, india) was studied for its taxonomic position. by 16s rrna gene sequence similarity comparison, it was shown that the isolate clearly grouped within the methylobacterium cluster. on the basis of pairwise comparison of the 16s rrna gene sequences, isolate gh-105t was most closely related to the type strains of methylobacterium ... | 2011 | 21378138 |
| isolation of methanobactin from the spent media of methane-oxidizing bacteria. | chalkophores are low molecular mass modified peptides involved in copper acquisition in methane-oxidizing bacteria (mob). a screening method for the detection of this copper-binding molecule is presented in chapter 16. here we describe methods to (1) maximize expression and secretion of chalkophores, (2) concentrate chalkophores from the spent media of mob, and (3) purify chalkophores. | 2011 | 21419927 |
| protection of arabidopsis thaliana against leaf-pathogenic pseudomonas syringae by sphingomonas strains in a controlled model system. | diverse bacterial taxa live in association with plants without causing deleterious effects. previous analyses of phyllosphere communities revealed the predominance of few bacterial genera on healthy dicotyl plants, provoking the question of whether these commensals play a particular role in plant protection. here, we tested two of them, methylobacterium and sphingomonas, with respect to their ability to diminish disease symptom formation and the proliferation of the foliar plant pathogen pseudom ... | 2011 | 21421777 |
| nodulation of crotalaria podocarpa dc. by methylobacterium nodulans displays very unusual features. | crotalaria are plants of the fabaceae family whose nodulation characteristics have been little explored despite the recent discovery of their unexpected ability to be efficiently nodulated in symbiosis with bacteria of the genus methylobacterium. it has been shown that methylotrophy plays a key role in this unusual symbiotic system, as it is expressed within the nodule and as non-methylotroph mutants had a depleting effect on plant growth response. within the nodule, methylobacterium is thus abl ... | 2011 | 21422120 |
| evaluation of pink-pigmented facultative methylotrophic bacteria for phosphate solubilization. | thirteen pink-pigmented facultative methylotrophic (ppfm) strains isolated from adyar and cooum rivers in chennai and forest soil samples in tamil nadu, india, along with methylobacterium extorquens, m. organophilum, m. gregans, and m. komagatae were screened for phosphate solubilization in plates. p-solubilization index of the ppfms grown on nbrip-bpb plates for 7 days ranged from 1.1 to 2.7. the growth of ppfms in tricalcium phosphate amended media was found directly proportional to the glucos ... | 2011 | 21445558 |
| isolation and genetic characterization of aurantimonas and methylobacterium strains from stems of hypernodulated soybeans. | the aims of this study were to isolate aurantimonas and methylobacterium strains that responded to soybean nodulation phenotypes and nitrogen fertilization rates in a previous culture-independent analysis (ikeda et al. isme j. 4:315-326, 2010). two strategies were adopted for isolation from enriched bacterial cells prepared from stems of field-grown, hypernodulated soybeans: pcr-assisted isolation for aurantimonas and selective cultivation for methylobacterium. thirteen of 768 isolates cultivate ... | 2011 | 21512309 |
| production of poly-β-hydroxybutyrate (phb) by methylobacterium organophilum isolated from a methanotrophic consortium in a two-phase partition bioreactor. | the biodegradation of methane, a greenhouse gas, and the accumulation of poly-β-hydroxybutyrate (phb) were studied using a methanotrophic consortium and an isolated strain thereof. the specific rates for methane consumption were 100 and [formula: see text] for the isolate and the consortium, respectively. also the effect of including 10% (vv(-1)) of silicone oil in a two-phase partitioning bioreactor (tppb) was assayed for the elimination of 1% methane in air stream. tppb allowed a 33-45% increa ... | 2011 | 21530080 |
| detection and isolation of chloromethane-degrading bacteria from the arabidopsis thaliana phyllosphere, and characterization of chloromethane utilization genes. | chloromethane gas is produced naturally in the phyllosphere, the compartment defined as the aboveground parts of vegetation, which hosts a rich bacterial flora. chloromethane may serve as a growth substrate for specialized aerobic methylotrophic bacteria, which have been isolated from soil and water environments, and use cmu genes for chloromethane utilization. evidence for the presence of chloromethane-degrading bacteria on the leaf surfaces of arabidopsis thaliana was obtained by specific quan ... | 2011 | 21545604 |
| endophytic bacteria in the rice plant. | endophytic bacteria are defined as bacteria detected inside surface-sterilized plants or extracted from inside plants and having no visibly harmful effects on the plants. various kinds of endophytic bacteria, such as pantoea, methylobacterium, azospirillum, herbaspirillum, burkholderia and rhizobium etc., have been found inside rice plants. this minireview summarizes and discusses recent studies of endophytic bacteria residing in rice plants, focusing on flora, origin, movement, and interaction ... | 2008 | 21558696 |
| rice seeds as sources of endophytic bacteria. | endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ... | 2009 | 21566368 |
| diversity of bacteria at healthy human conjunctiva. | purpose. ocular surface (os) microbiota contributes to infectious and autoimmune diseases of the eye. comprehensive analysis of microbial diversity at the os has been impossible because of the limitations of conventional cultivation techniques. this pilot study aimed to explore true diversity of human os microbiota using dna sequencing-based detection and identification of bacteria. methods. composition of the bacterial community was characterized using deep sequencing of the 16s rrna gene ampli ... | 2011 | 21571682 |
| tonb-dependent receptors in nitrogen-fixing nodulating bacteria. | tonb-dependent receptors (tbdrs) allow gram-negative bacteria to uptake scarce resources from competitive environments with very high affinity. early reports on tbdrs focused on the uptake of siderophore-iron complexes but recent studies have showed that the spectrum of ligands includes sugars, vitamins, heme, and other non-ferrous cations. to investigate the possible roles of tbdrs in nitrogen-fixing, nodulating bacteria, a bioinformatics approach was adopted to identify their presence in the g ... | 2010 | 21576856 |
| loss of allostery and coenzyme b(12) delivery by a pathogenic mutation in adenosyltransferase. | atp-dependent cob(i)alamin adenosyltransferase (atr) is a bifunctional protein: an enzyme that catalyzes the adenosylation of cob(i)alamin and an escort that delivers the product, adenosylcobalamin (adocbl or coenzyme b(12)), to methylmalonyl-coa mutase (mcm), resulting in holoenzyme formation. failure to assemble holo-mcm leads to methylmalonic aciduria. we have previously demonstrated that only 2 equiv of adocbl bind per homotrimer of atr and that binding of atp to the vacant active site trigg ... | 2011 | 21604717 |
| methylobacterium bullatum sp. nov., a methylotrophic bacterium isolated from funaria hygrometrica. | a novel, pink-pigmented aerobic, facultatively methylotrophic bacterial strain (f3.2(t)) isolated from the phyllosphere of funaria hygrometrica, was analyzed using a polyphasic approach. cells were gram-negative, motile rods, strictly aerobic and non-spore-forming and exhibited surface structures varying in quantity, distribution and morphology. the isolate grew at 10-33°c over a ph range of 5.5-8.0 and in the presence of less than 1.0% nacl. strain f3.2(t) shared less than 70% dna-dna binding t ... | 2011 | 21612883 |
| evolution. in evolution, the sum is less than its parts. | | 2011 | 21636764 |
| diminishing returns epistasis among beneficial mutations decelerates adaptation. | epistasis has substantial impacts on evolution, in particular, the rate of adaptation. we generated combinations of beneficial mutations that arose in a lineage during rapid adaptation of a bacterium whose growth depended on a newly introduced metabolic pathway. the proportional selective benefit for three of the four loci consistently decreased when they were introduced onto more fit backgrounds. these three alleles all reduced morphological defects caused by expression of the foreign pathway. ... | 2011 | 21636771 |
| introducing a new bioengineered bug: methylobacterium extorquens tuned as a microbial bioplastic factory. | discussion on and use of methanol as chemical feedstock and as alternative fuel has gained momentum during the past years. consequently, microorganism and product design based on ''methylotrophism'' is in vogue as reflected by increasing research and development activities in methanol-related areas. a recent example of microorganism and product development is the use of recombinant methylobacterium extorquens atcc 55366 strains in the production of second generation biopolyesters. feeding n-alke ... | 2011 | 21636993 |
| methylobacterium cerastii sp. nov., a novel species isolated from the leaf surface of cerastium holosteoides. | two gram-negative non endospore-forming rod-like strains, designated c15(t) and c44, were isolated from the phyllosphere of cerastium holosteoides and were studied in detail in order to assess their taxonomic position. 16s rrna gene sequence analysis allocated both isolates clearly to the genus methylobacterium. both strains revealed the highest 16s rrna gene sequence similarity to m. marchantiae jt1(t) (97.5%) and m. jeotgali 52/203-9(t) (97.4%). the fatty acids profiles contained major amounts ... | 2011 | 21669927 |
| a novel growth-promoting microbe, methylobacterium funariae sp. nov., isolated from the leaf surface of a common moss. | land plants (embryophytes) evolved in the presence of prokaryotic microbes. as a result, numerous mutually beneficial associations (symbioses) developed that can be analyzed using a variety of methods. here we describe the isolation and characterization of a new pink-pigmented facultatively methylotrophic symbiotic bacterium of the genus methylobacterium (laboratory strain f3.2) that was isolated from the gametophytic phylloids of the common cord moss funaria hygrometrica hedw. plantlets were co ... | 2011 | 21673511 |
| pole age affects cell size and the timing of cell division in methylobacterium extorquens am1. | a number of recent experiments at the single-cell level have shown that genetically identical bacteria that live in homogeneous environments often show a substantial degree of phenotypic variation between cells. often, this variation is attributed to stochastic aspects of biology - the fact that many biological processes involve small numbers of molecules, and are thus inherently variable. however, not all variation between cells needs to be stochastic in nature; one deterministic process that c ... | 2011 | 21784923 |
| isolation of estrogen-degrading bacteria from an activated sludge bioreactor treating swine waste, including a strain that converts estrone to ß-estradiol. | an estrogen-degrading bacterial consortium from a swine wastewater biotreatment was enriched in the presence of low concentrations (1 mg/l) of estrone (e1), 17ß-estradiol (ße2), and equol (eqo) as sole carbon sources. the consortium removed 99% ± 1% of these three estrogens in 48 h. estrogen removal occurred even in the presence of an ammonia monooxygenase inhibitor, suggesting that nitrifiers are not involved. five strains showing estrogen-metabolizing activity were isolated from the consortium ... | 2011 | 21770814 |
| the effects of metabolite molecules produced by drinking water-isolated bacteria on their single and multispecies biofilms. | the elucidation of the mechanisms by which diverse species survive and interact in drinking water (dw) biofilm communities may allow the identification of new biofilm control strategies. the purpose of the present study was to investigate the effects of metabolite molecules produced by bacteria isolated from dw on biofilm formation. six opportunistic bacteria, viz. acinetobacter calcoaceticus, burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata and staph ... | 2011 | 21732713 |
| detection of dichloromethane with a bioluminescent (lux) bacterial bioreporter. | the focus of this research effort was to develop an autonomous, inducible, lux-based bioluminescent bioreporter for the real-time detection of dichloromethane. dichloromethane (dcm), also known as methylene chloride, is a volatile organic compound and one of the most commonly used halogenated solvents in the u.s., with applications ranging from grease and paint stripping to aerosol propellants and pharmaceutical tablet coatings. predictably, it is released into the environment where it contamina ... | 2011 | 21688172 |
| infections caused by unusual methylobacterium species. | we describe six patients with hospital-acquired bacteremia caused by methylobacterium species, including m. radiotolerans (n = 2), m. thiocyanatum (n = 2), m. aminovorans (n = 1), and m. lusitanum (n = 1), which were confirmed to species level by 16s rrna gene sequence analysis. among these patients, five had catheter-related bacteremia and all had favorable outcomes. | 2011 | 21734032 |
| The Ethylmalonyl-CoA Pathway Is Used in Place of the Glyoxylate Cycle by Methylobacterium extorquens AM1 during Growth on Acetate. | Acetyl-CoA assimilation was extensively studied in organisms harboring the glyoxylate cycle. In this study, we analyzed the metabolism of the facultative methylotroph Methylobacterium extorquens AM1, which lacks isocitrate lyase, the key enzyme in the glyoxylate cycle, during growth on acetate. MS/MS-based proteomic analysis revealed that the protein repertoire of M. extorquens AM1 grown on acetate is similar to that of cells grown on methanol and includes enzymes of the ethylmalonyl-CoA (EMC) p ... | 2012 | 22105076 |
| XoxF is required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1. | In Gram-negative methylotrophic bacteria, the first step in methylotrophic growth is the oxidation of methanol to formaldehyde in the periplasm by methanol dehydrogenase. In most organisms studied to date, this enzyme consists of the MxaF and MxaI proteins, which make up the large and small subunits of this heterotetrameric enzyme. The Methylobacterium extorquens AM1 genome contains two homologs of MxaF, XoxF1 and XoxF2, which are ~50% identical to MxaF and ~90% identical to each other. It was p ... | 2011 | 21873495 |
| Geosmin degradation by seasonal biofilm from a biological treatment facility. | INTRODUCTION: Initial geosmin degradation was closely related to water temperature and natural geosmin concentration of sampling environment. Here, for the first time, we evaluated the biodegradation of geosmin by microorganisms in biofilm from biological treatment unit of actual potable water treatment plant. MATERIALS AND METHODS: At an initial geosmin concentration of 2,500 ng/l, efficient geosmin removal was confirmed throughout the year. Furthermore, in the presence of mixed musty odor comp ... | 2011 | 21912954 |
| [Methylobacterium podarium isolated in the course of intraocular inflammation]. | | 2011 | 22055613 |
| Evidence of Methylobacterium spp. and Hyphomicrobium sp. in azaspiracid toxin contaminated mussel tissues and assessment of the effect of azaspiracid on their growth. | A flagellar protein belonging to the genus Methylobacterium or Agrobacterium was previously observed by proteomics in azaspiracids (AZA) toxic mussels. Here, we report the isolation of two different Methylobacterium spp. (NTx1 and Tx1) from non-toxic and AZA toxic mussels, respectively, which when co-cultured with AZA exhibited significantly different growth responses - isolate Tx1 growth rate was enhanced, whereas growth of isolate NTx1 was adversely affected, compared to non-AZA supplemented c ... | 2011 | 21982824 |
| metaproteogenomic analysis of microbial communities in the phyllosphere and rhizosphere of rice. | the above- and below-ground parts of rice plants create specific habitats for various microorganisms. in this study, we characterized the phyllosphere and rhizosphere microbiota of rice cultivars using a metaproteogenomic approach to get insight into the physiology of the bacteria and archaea that live in association with rice. the metaproteomic datasets gave rise to a total of about 4600 identified proteins and indicated the presence of one-carbon conversion processes in the rhizosphere as well ... | 2011 | 22189496 |
| methylobacterium oxalidis sp. nov., isolated from leaves of oxalis corniculata. | a pink-pigmented, facultatively methylotrophic bacterium, strain 35a(t), was isolated from the leaves of oxalis corniculata. the cells of strain 35a(t) are gram-reaction-negative, motile, non-spore-forming rods. the highest 16s rrna gene pairwise sequence similarities for strain 35a(t) were found with the type strains of methylobacterium iners (96.7%), m. soli (96.6%) and m. jeotgali (96.3%). 16s rrna gene sequence similarities with the type strains of all other methylobacterium species were bel ... | 2011 | 21908681 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| characterization of methylobacterium strains isolated from the phyllosphere and description of methylobacterium longum sp. nov. | methylobacterium strains are abundantly found in the phyllosphere of plants. morphological, physiological and chemotaxonomical properties of 12 previously isolated strains were analyzed in order to obtain a more detailed overview of the characteristics of phyllosphere colonizing methylobacterium strains. all strains showed the typical properties of the genus methylobacterium, including pink pigmentation, facultative methylotrophy, a fatty acid profile dominated by c18:1 ω7c, and a high g+c conte ... | 2012 | 21986935 |
| genome-scale reconstruction and system level investigation of the metabolic network of methylobacterium extorquens am1. | methylotrophic microorganisms are playing a key role in biogeochemical processes - especially the global carbon cycle - and have gained interest for biotechnological purposes. significant progress was made in the recent years in the biochemistry, genetics, genomics, and physiology of methylotrophic bacteria, showing that methylotrophy is much more widespread and versatile than initially assumed. despite such progress, system-level description of the methylotrophic metabolism is currently lacking ... | 2011 | 22074569 |
| 2,4,5-trichlorophenol degradation using a novel tio2-coated biofilm carrier: roles of adsorption, photocatalysis, and biodegradation. | intimate coupling of photocatalysis and biodegradation (icpb) offers potential for degrading biorecalcitrant and toxic organic compounds. this study reports on a novel sponge-type, tio(2)-coated biofilm carrier that showed significant adherence of tio(2) and ability to accumulate biomass in its interior. this carrier was tested for icpb in a continuous-flow photocatalytic circulating-bed biofilm reactor (pcbbr) to mineralize 2,4,5-trichlorophenol (tcp), which is biorecalcitrant. four mechanisms ... | 2011 | 21861477 |
| [phytosymbiosis of aerobic methylobacteria: new facts and views ]. | | 2011 | 22073542 |
| genotyping and chlorine-resistance of methylobacterium aquaticum isolated from water samples in japan. | for microbial ecological analysis, 14 strains of methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and rapd-pcr tests. the ribopatterns after digestion with ecori obtained from 14 strains of m. aquaticum were used to divide the strains into two groups (groups i and ii) with a similarity of 55%. from the analysis of rapd patterns using primer 208, the 14 strains were divided into 3 groups (a-c) based on a homology of 45% or gre ... | 2011 | 21946320 |
| microbial biofilms on the sandstone monuments of the angkor wat complex, cambodia. | discoloring biofilms from cambodian temples angkor wat, preah khan, and the bayon and west prasat in angkor thom contained a microbial community dominated by coccoid cyanobacteria. molecular analysis identified chroococcidiopsis as major colonizer, but low similarity values (<95%) suggested a similar genus or species not present in the databases. in only two of the six sites sampled were filamentous cyanobacteria, microcoleus, leptolyngbya, and scytonema, found; the first two detected by sequenc ... | 2011 | 22006074 |
| functional genomics of dichloromethane utilization in methylobacterium extorquens dm4. | dichloromethane (ch(2)cl(2) , dcm) is a chlorinated solvent mainly produced by industry, and a common pollutant. some aerobic methylotrophic bacteria are able to grow with this chlorinated methane as their sole carbon and energy source, using a dcm dehalogenase/glutathione s-transferase encoded by dcma to transform dcm into two molecules of hcl and one molecule of formaldehyde, a toxic intermediate of methylotrophic metabolism. in methylobacterium extorquens dm4 of known genome sequence, dcma li ... | 2011 | 21854516 |
| methylobacterium gnaphalii sp. nov., isolated from leaves of gnaphalium spicatum. | a pink-pigmented, facultatively methylotrophic bacterium, strain 23et, was isolated from the leaves of gnaphalium spicatum. the cells of strain 23et are gram-reaction-negative, motile, non-spore-forming rods. on the basis of 16s rrna gene sequence similarity, strain 23et was related to methylobacterium organophilum atcc 27886t (97.1%) and m. marchantiae jt1t (97%), and the phylogenetic similarities to all other methylobacterium species with validly published names were less than 97%. major cellu ... | 2011 | 22199216 |
| diversity and biogeography of selected phyllosphere bacteria with special emphasis on methylobacterium spp. | on the basis of cultivation-dependent (isolation on mineral salt medium supplemented with 0.5% methanol) and -independent (dgge analysis) methods, we investigated the influence of the host plant species trifolium repens and cerastium holosteoides, three geographic locations and the land-use types meadow, mown pasture and pasture on the abundance and community composition of selected phyllosphere bacteria with emphasis on methylobacterium species. methylobacterium abundance was significantly high ... | 2011 | 22000032 |
| microvirga lupini sp. nov., microvirga lotononidis sp. nov., and microvirga zambiensis sp. nov. are alphaproteobacterial root nodule bacteria that specifically nodulate and fix nitrogen with geographically and taxonomically separate legume hosts. | strains of gram-negative, rod-shaped, non-spore-forming bacteria were isolated from nitrogen-fixing nodules of the native legumes listia angolensis (from zambia) and lupinus texensis (from texas, usa). phylogenetic analysis of the 16s rrna gene showed that the novel strains belong to the genus microvirga, with 96.1 % or greater sequence similarity with type strains of this genus. the closest relative of the representative strains lut6t and wsm3557t was m. flocculans tfbt, with 97.6-98.0 % simila ... | 2011 | 22199210 |
| Epiphytic pink-pigmented methylotrophic bacteria enhance germination and seedling growth of wheat (Triticum aestivum) by producing phytohormone. | Methylotrophic bacteria were isolated from the phyllosphere of different crop plants such as sugarcane, pigeonpea, mustard, potato and radish. The methylotrophic isolates were differentiated based on growth characteristics and colony morphology on methanol supplemented ammonium mineral salts medium. Amplification of the mxaF gene helped in the identification of the methylotrophic isolates as belonging to the genus Methylobacterium. Cell-free culture filtrates of these strains enhanced seed germi ... | 2011 | 22200783 |
| rhodoferax-related pufm gene cluster dominates the aerobic anoxygenic phototrophic communities in german freshwater lakes. | the presence of aerobic anoxygenic phototrophs (aaps) has been repeatedly reported from various marine environments, but their distribution in freshwater lakes was neglected until recently. we investigated the phylogenetic composition of aap communities in 10 lakes in northeastern germany with different trophic status including oligotrophic lake stechlin and humic matter rich lake grosse fuchskuhle. the aap community was composed by members of alpha- and betaproteobacteria, but their contributio ... | 2011 | 21895915 |