| alpha-3-ketoglucosidase of agrobacterium tumefaciens. | a 3-ketosucrose-degrading enzyme was purified 80-fold from the sonic extracts of agrobacterium tumefaciens iam 1525 grown on a sucrose-containing medium. the enzyme catalyzes hydrolysis of alpha-3-ketoglucosides such as 3-ketosucrose, 3-ketotrehalose, 3-ketomaltose, and 3-ketoglucose-1-phosphate but not of beta-3-ketoglucosides, beta-3-ketogalactosides, and other glycosides such as sucrose, trehalose, maltose, glucose-1-phosphate, cellobiose, lactose, or raffinose. from the strict substrate spec ... | 1970 | 5438043 |
| biosynthesis of bacterial glycogen. 8. activation and inhibition of the adenosine diphosphoglucose pyrophosphorylase of rhodopseudomonas capsulata and of agrobacterium tumefaciens. | | 1970 | 5460185 |
| [intervention in the inoculation wound of bacteria belonging to various strains of agrobacterium tumefaciens (smith and town) conn]. | | 1970 | 5478546 |
| specificity patterns of agrobacterium tumefaciens phages. | | 1970 | 5485742 |
| liberation of agrobacterium tumefaciens dna from the crown gall tumor cell dna by shearing. | | 1970 | 5495741 |
| [comparative study of the formation and biogenesis of beta-indoleacetic acid by agrobacterium tumefaciens and its fixed l forms]. | | 1970 | 5499549 |
| comments on the results of the preceding publication of manigault concerning agrobacterium tumefaciens m39 mutant. | | 1970 | 5512790 |
| some characteristic properties concerning agrobacterium tumefaciens m39 mutant. | | 1970 | 5519706 |
| the loss of tumor-initiating ability in agrobacterium tumefaciens by incubation at high temperature. | | 1971 | 5544763 |
| conditions for production of 3-ketomaltose form agrobacterium tumefaciens. | | 1971 | 5549698 |
| agrobacterium tumefaciens ribonucleic acid synthesis in tomato cells and crown gall induction. | purified agrobacterium tumefaciens deoxyribonucleic acid (dna) does not produce crown gall tumors in growing plants, conditioned by wounding, as the living bacteria do. purified bacterial dna migrates in the plant and replicates, but it is not transcribed in our experimental conditions. on the contrary, when dna is released naturally from bacteria into plant cells, a bacterial ribonucleic acid (rna) can be found in these cells. there seems to be a direct relation between the appearance of a. tum ... | 1971 | 5573736 |
| chemical composition of cell envelopes from agrobacterium tumefaciens. | | 1967 | 5582205 |
| [various characteristics of fixed l forms of agrobacterium tumefaciens induced by ultraviolet radiation]. | | 1967 | 5619837 |
| agrobacterium tumefaciens conn. iv. bacteriophage pb21 and its inhibitory effect on tumor induction. | strain b2 of agrobacterium tumefaciens conn produces plaques when seeded against strain b6-806 of the same organism. from such a plaque, a highly virulent bacteriophage was obtained by use of d'herelle's technique of selecting for virulent phage. on nutrient agar, this phage, pb2(1), produced large clear plaques which did not overgrow. plaques produced on a glutamate medium and on white's plant tissue culture medium were even larger and in white's medium had a three-dimensional appearance. pb2(1 ... | 1967 | 5623963 |
| murine toxicit of agrobacterium tumefaciens. | eleven strains of the crown gall organism, agrobacterium tumefaciens, tested by intraperitoneal injection into mice, were lethal within 48 hr. five other species had some lethal strains. the lethal effect of a. tumefaciens appeared to be the result of a toxic rather than an infectious process, since histopathological anomalies were not found in mice injected with live cultures and since heat-killed cultures were lethal. the murine toxin disappeared when a. tumefaciens was grown at 36 c and reapp ... | 1968 | 5643064 |
| methylation of the cellular lipid of methionine-requiring agrobacterium tumefaciens. | mutants of agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of n-methyl-n'-nitro-n-nitrosoguanidine. in addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumarate. when ((14)c-methyl)-methionine was added to strain wm-11 growing in a prescribed basal medium, incorporation of (14)c into the cellular "residue" fraction and polar "n-methylate ... | 1968 | 5669890 |
| [bacteriophages of agrobacterium tumefaciens (smith and town.) conn. i. isolation and characteristics]. | | 1968 | 5672117 |
| [bacteriophages of agrobacterium tumefaciens. ii. lysogenic strain b6m in the tissues of pisum sativum l, and the effect of glycine on phage production]. | | 1968 | 5674588 |
| ultraviolet repair capabilities of agrobacterium tumefaciens. | | 1968 | 5681510 |
| activation of the tumor-inducing ability of agrobacterium tumefaciens (smith and townsend) conn. by means of capsicin. | | 1968 | 5709363 |
| hexopyranoside: cytochrome c oxidoreductase from agrobacterium tumefaciens. | | 1968 | 5726844 |
| [effect of maleic hydrazide on the agrobacterium tumefaciens. i. study of the action of maleic hydrazide on the growth and synthesis of beta-indolacetic acid]. | | 1968 | 5731452 |
| [influence of maleic hydrazines on the agrobacterium tumefaciens. ii. formation of tumors due to the action of maleic hydrazine on the bacteria and precursors of beta-indolacetic acid]. | | 1968 | 5731453 |
| bacterial attachment to a specific wound site as an essential stage in tumor initiation by agrobacterium tumefaciens. | the number of tumors initiated by agrobacterium tumefaciens strain b6 on primary pinto bean leaves was decreased when cells of an avirulent strain (iibnv6) were included in the inoculum. with sufficient b6 cells to initiate ca. 50% of the maximal number of tumors per leaf, inhibition was detected at a 1:1 ratio of b6 to iibnv6 cells and increased linearly with the logarithm of the number of iibnv6. varying the number of b6 in the presence of a constant number of iibnv6 or varying the number of b ... | 1969 | 5773014 |
| bacteriophage release in a lysogenic strain of agrobacterium tumefaciens. | bacteriophage release in a lysogenic strain of agrobacterium tumefaciens v-1 is temperature-sensitive. at 25 c and 30 c, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 c, although bacterial growth was not inhibited, phage release was suppressed. phage synthesis was induced by heat shock, 42 c for 30 min, ultraviolet irradiation, and mitomycin c. induction by ultraviolet light was unusual-an immediate rise in phage titer followed irradiation. a large increase occur ... | 1969 | 5774138 |
| methylation of fatty acids in a methionine-dependent agrobacterium tumefaciens controlled with exogenous methionine. | | 1969 | 5811212 |
| the quantitative determination of the infectivity of agrobacterium tumefaciens. | | 1965 | 5830983 |
| on the extracellular accumulation and isolation of d-ribuloxe in cultures of agrobacterium tumefaciens. | | 1965 | 5834715 |
| aminotransferases of agrobacterium tumefaciens. transamination between tryptophan and phenylpyruvate. | | 1964 | 5837443 |
| phosphatidylcholine synthesis in agrobacterium tumefaciens. ii. uptake and utilization of choline. | | 1965 | 5842051 |
| auxotrophic mutation and infectivity of agrobacterium tumefaciens. | | 1965 | 5847803 |
| on the active transport of sucrose and of 3-keto-sucrose in agrobacterium tumefaciens. | | 1965 | 5855652 |
| [studies of the genetic transformation between agrobacterium tumefaciens and rhizobium sp. 3. comparative analysis of the dna from the partners in a transformation]. | | 1965 | 5875029 |
| agrobacterium tumefaciens conn. 3. effect of thermal shock on bacteria in relation to tumorinducing ability. | stonier, tom (manhattan college, bronx, n.y.), robert e. beardsley, lowell parsons, and james mcsharry. agrobacterium tumefaciens conn. iii. effect of thermal shock on bacteria in relation to tumor-inducing ability. j. bacteriol. 91:266-269. 1966.-bacteria heated to 42 c for 30 min exhibit a decrease in tumor-initiating ability without a detectable loss in viability. the thermal shock inhibits subsequent bacterial growth for up to 1.5 hr. as bacterial growth recovers, so does tumor-initiating ab ... | 1966 | 5903095 |
| isolation and morphology of temperature agrobacterium tumefaciens bacteriophage. | zimmerer, robert p. (the pennsylvania state university, university park), robert h. hamilton, and christine pootjes. isolation and morphology of temperate agrobacterium tumefaciens bacteriophage. j. bacteriol. 92:746-750. 1966.-lysogeny was detected in 14 strains of agrobacterium tumefaciens among 130 bacterial strains tested with strain b-6 used as the host. partial lysis was observed with 13 additional bacterial strains. morphological studies of five strains showed that the phage had similar f ... | 1966 | 5922546 |
| mechanisms of tumor induction in crown gall. i. production and pathogenicity of spheroplasts of agrobacterium tumefaciens. | | 1966 | 5923158 |
| characteristics of agrobacterium tumefaciens auxotrophic mutant infectivity. | lippincott, barbara b. (northwestern university, evanston, ill.), and james a. lippincott. characteristics of agrobacterium tumefaciens auxotrophic mutant infectivity. j. bacteriol. 92:937-945. 166.-mutants of agrobacterium tumefaciens auxotrophic for adenine, methionine, or asparagine are less infectious than the wild-type strain b6 from which they were derived and show increased infectivity on pinto bean leaves when the specific compounds required for growth of the mutants are added to the inf ... | 1966 | 5926761 |
| requirements for and rates of formation of extracellular pseudouridine by agrobacterium tumefaciens. | | 1966 | 5929154 |
| [on the production of biotin and riboflavin by different strains of agrobacterium tumefaciens (smith and town.) conn]. | | 1966 | 5936223 |
| [comparative study of the fat component of agrobacterium tumefaciens and its fixed l forms. ii. variations according to the method of cultivation and study of the phospholipids]. | | 1966 | 5940294 |
| on the biosynthesis of pseudouridine and of pseudouridylic acid in agrobacterium tumefaciens. | | 1966 | 5942965 |
| fixed l-forms of agrobacterium tumefaciens induced by ultra-violet light radiation. | | 1966 | 5956332 |
| [the mode of action of glycine on the inhibition of growth of agrobacterium tumefaciens. i. effects on the metabolism of messenger rna of the strain b6]. | | 1966 | 5965734 |
| [on the testing of virulence in agrobacterium tumefaciens]. | | 1966 | 5985353 |
| metabolism of amino acids in agrobacterium tumefaciens. ii. uptake of l-valine by growing cells. | | 1967 | 6021174 |
| time required for tumour initiation by agrobacterium tumefaciens on pinto bean leaves. | | 1967 | 6032253 |
| ultraviolet-induced changes in the infectivity of agrobacterium tumefaciens. | the infectivity of agrobacterium tumefaciens strain b6 irradiated with short-wavelength ultraviolet light was followed as a function of dose. previously reported enhancements of b6 infectivity by ultraviolet irradiation, in samples inoculated after 1.75 hr of dark incubation at 27 c, or immediately following irradiation, were found to occur most frequently after losses in cell viability of 60% and of 90% or more, respectively. changes in colony-forming ability and tumor-initiating ability with i ... | 1967 | 6032504 |
| purification and properties of 3-ketosucrose-forming enzyme from the cells of agrobacterium tumefaciens. | | 1967 | 6038493 |
| [effects of boric acid on the crown-gall bacterium, agrobacterium tumefaciens (smith and town) conn]. | | 1967 | 6055850 |
| enhancement of agrobacterium tumefaciens infectivity by mitomycin c. | the ability of agrobacterium tumefaciens to induce pinto leaf tumors may be enhanced two- to threefold after treatment with mitomycin c. the enhancement may be obtained with either lethal or nonlethal concentrations. with 10-min treatments, an optimal response was obtained with 0.005 mug of mitomycin c per ml in the absence of any change in the number of viable cells. both the tumor induction process and the tumors induced by treated cultures appear qualitatively the same as controls. to account ... | 1967 | 6057802 |
| formation of complexes between dna isolated from tobacco crown gall tumours and rna complementary to agrobacterium tumefaciens dna. | | 1967 | 6064642 |
| [action of capsicine on the multiplication and virulence of agrobacterium tumefaciens]. | | 1967 | 6077893 |
| metabolism of amino acids in agrobacterium tumefaciens. 3. uptake of l-pro- line. | | 1967 | 6082570 |
| a comparison of virulence determinants in an octopine ti plasmid, a nopaline ti plasmid, and an ri plasmid by complementation analysis of agrobacterium tumefaciens mutants. | transposon-insertion mutants with vir- ti plasmids were characterized and then used in complementation experiments. one of the mutants (lba 1517) had a mutation in a newly discovered vir locus called virf. the virf mutation led to a strongly diminished virulence on tomato and tobacco, but not on certain other plant species. also a mutant (lba 1505) was isolated with a mutation somewhere in the bacterial genome but outside the octopine ti plasmid that caused a restriction in host range for tumor ... | 1984 | 6087390 |
| genetic and molecular characterization of the pseudomonas plasmid pvs1. | a restriction map of the 30-kb nonconjugative pseudomonas plasmid pvs1 was constructed. derivatives of pvs1 obtained in vitro by successive deletions were used to localize on the physical map the determinant for resistance to mercuric ions (carried by transposon tn501), the gene(s) encoding sulfonamide resistance, a 1.6-kb region affecting plasmid stability and establishment in p. fluorescens atcc 13525, and a segment required for mobilization of pvs1 by plasmid rp1. the sulfonamide resistance d ... | 1984 | 6087391 |
| [taxonomic position of agrobacterium strains of hospital origin]. | a collection of 31 strains received as agrobacterium tumefaciens and a. radiobacter was subjected to detailed phenotypic and genomic studies. these strains were recovered from plants, soil, water and clinical specimens. type strains of a. tumefaciens, a. radiobacter, a. rhizogenes and a. rubi were also included. the strains were tested for their ability to use 169 organic compounds as sources of carbon and energy. in addition, 11 conventional characters were studied for each strain. relatedness ... | 1984 | 6087709 |
| comparison of t-dna oncogene complements of agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. | the t-dna oncogene complements of the limited-host-range tumor-inducing plasmid ptiag63 and the wide-host-range plasmid ptia6 were compared. the resulting data indicate that ptiag63 has dna sequences related to most of the genes encoded by the oncogene region, the tl-dna, of ptia6 and that these sequences are divided between two t-dna regions, the ta-dna, which encoded sequences related to ptia6 genes 4 (the cytokinin independence gene) and 6a, as well as to a ptia6 tl-dna fragment that encoded ... | 1984 | 6090421 |
| host range encoded by the agrobacterium tumefaciens tumor-inducing plasmid ptiag63 can be expanded by modification of its t-dna oncogene complement. | agrobacterium tumefaciens harboring ptia6 incite unorganized tumors on nicotiana rustica, sunflowers, carrots, and tomatoes, whereas isogenic strains of agrobacteria harboring ptiag63 form "rooty" tumors on n. rustica and are essentially avirulent on sunflowers, carrots, and tomatoes. in this report we show that the different host range characteristics of these two plasmids were due, in part, to differences in the t-dna oncogene complements of the plasmids. specifically, we constructed derivativ ... | 1984 | 6090422 |
| t-dna of agrobacterium tumefaciens encodes an enzyme of cytokinin biosynthesis. | phytohormone overproduction in crown gall tumors is due to the expression of several t-dna genes. the data strongly suggest that the tmr gene (transcript 4) is responsible for cytokinin overproduction by encoding dimethylallyl-pyrophosphate:amp dimethylallyltransferase (dma transferase), an enzyme directly involved in cytokinin biosynthesis. cell-free extracts of escherichia coli strains containing the tmr gene from ptia6nc had dma transferase activity. no activity was present in the control str ... | 1984 | 6091129 |
| physical and functional map of an agrobacterium tumefaciens tumor-inducing plasmid that confers a narrow host range. | agrobacterium tumefaciens ag162 induces crown gall disease on an unusually narrow range of host plants. the 231-kilobase ti plasmid which has been shown to determine host range, was subcloned into the vector pvck102. by comparing overlaps of cloned insets, maps were constructed for the restriction endonucleases sali, xhoi, ecori, and kpni. plasmid incompatibility, octopine catabolism, and at least six virulence genes were localized. plasmid incompatibility between ptiag162 and the wide host rang ... | 1984 | 6094476 |
| binary agrobacterium vectors for plant transformation. | a vector molecule for the efficient transformation of higher plants has been constructed with several features that make it efficient to use. it utilizes the trans acting functions of the vir region of a co-resident ti plasmid in agrobacterium tumefaciens to transfer sequences bordered by left and right t-dna border sequences into the nuclear genome of plants. the t-region contains a dominant selectable marker gene that confers high levels of resistance to kanamycin, and a lac alpha-complementin ... | 1984 | 6095209 |
| nucleotide sequence of the insertion sequence found in the t-dna region of mutant ti plasmid ptia66 and distribution of its homologues in octopine ti plasmid. | the octopine tumor-inducing (ti) plasmid ptia66 has an insertion mutation in its t region (the dna region incorporated into the plant genome) that results in the slow growth of crown gall tumors. these tumors exhibit hormonal autonomy different from that of the crown gall tumors caused by wild-type ti plasmids. in the present study, the nucleotide sequences of both the dna segment inserted into ptia66 and its target site have been determined. the inserted segment is 2548 base pairs long and has ... | 1984 | 6095299 |
| design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of agrobacterium tumefaciens plasmid ptic58. | the construction of a set of new plasmids that are suitable as general cloning vectors in escherichia coli and agrobacterium tumefaciens is described. plasmid pucd2 is amplifiable in e. coli, replicates in a wide range of gram-negative hosts and contains a number of useful restriction endonuclear cleavage sites and antibiotic resistance genes. this includes unique sites for kpni, saci, sacii, psti, clai, sali, ecorv, and pvuii and the genes for resistance to kanamycin, tetracycline, ampicillin, ... | 1984 | 6095350 |
| a cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells. | the promoter region of the camv inclusion body protein gene was modified for use in chimeric gene fusions. the modified promoter was used to construct a selectable marker for plant transformation based on the tn 5 kanamycin resistance gene. this chimeric selectable marker was introduced into plant cells using oncogenic and deoncogenized strains of agrobacterium tumefaciens. both types of transformation produced kanamycin-resistant cell lines. the resistant cell lines derived from the deoncogeniz ... | 1984 | 6099400 |
| molecular mechanism of ti plasmid mobilization by r plasmids: isolation of ti plasmids with transposon-insertions in agrobacterium tumefaciens. | | 1980 | 6100904 |
| localization of the replication control region on the physical map of the octopine ti plasmid. | a series of small plasmids has been derived from the octopine plasmid pti-b6 by in vitro manipulation. the smallest plasmid that is able to replicate in agrobacterium tumefaciens contains the ampicillin-resistance determinant from tn1, coupled to a piece of dna that is homologous to hpai fragment number 11 of the octopine ti plasmid pti-ach 5. the incompatibility functions are also specified by this region. | 1980 | 6100931 |
| comparative properties of glutamine synthetases i and ii in rhizobium and agrobacterium spp. | some properties of glutamine synthetase i (gsi) and gsii are described for a fast-growing rhizobium sp. (rhizobium trifolii t1), a slow-growing rhizobium sp. (rhizobium japonicum usda 83), and agrobacterium tumefaciens c58. gsii of the fast-growing rhizobium sp. and gsii of the agrobacterium sp. were considerably more heat labile than gsii of the slow-growing rhizobium sp. as previously shown in r. japonicum 61a76, gsi became adenylylated rapidly in all species tested in response to ammonium. gs ... | 1980 | 6107288 |
| the interaction of agrobacterium ti-plasmid dna and plant cells. | the tumour-inducing plasmids of agrobacterium tumefaciens (ti-plasmids) reveal several interesting properties. they are catabolic plasmids, which, instead of rendering agrobacterium strains capable of catabolizing compounds found in nature, force a plant to synthesize these catabolites (denoted 'opines'). this situation is obtained by insertion of a segment of the ti-plasmid (the t-dna) into the plant nucleus, where t-dna genes become expressed and intervene in the biosynthesis of these opines. ... | 1980 | 6109298 |
| t-dna of pti-15955 from agrobacterium tumefaciens is transcribed into a minimum of seven polyadenylated rnas in a sunflower crown gall tumor. | northern blot hybridization analysis of polysomal polyadenylated rna isolated from sunflower crown gall tumor pscg-15955 demonstrated that a minimum of seven rnas were transcribed from t-dna of pti-15955 from agrobacterium tumefaciens. the sizes of the t-dna transcripts were 1.8, 1.6, 1.5, 1.1, 1.0 kilo bases (kb) and two transcripts of 0.8 kb long. the relative abundance of these polyadenylated rnas varied greatly, the 1.0 kb rna being the most abundant and the 1.6 kb rna being the least abunda ... | 1982 | 6175956 |
| biochemical studies on cytoplasmic membranes of agrocin sensitive and resistant strains of agrobacterium tumefaciens. | | 1982 | 6184960 |
| studies on superoxide dismutase activities in virulent and avirulent strains of agrobacterium tumefaciens and also in normal and crown gall tumor cells of bryophyllum calycinum. | superoxide dismutase activity in virulent strains of agrobacterium tumefaciens was found to be higher than that in avirulent strains. polyacrylamide gel electrophoresis revealed two isoenzymes in both these strains. these isoenzymes are suggested to be iron and manganese containing superoxide dismutases. crown gall tumor cells of the plant bryophyllum calycinum were found to have higher superoxide dismutase activity than the normal plant cells. polyacrylamide gel electrophoresis revealed two iso ... | 1982 | 6188336 |
| the role of cytosine methylation in the control of nopaline synthase gene expression in a plant tumor. | the ft37/1 tumor line induced by agrobacterium tumefaciens on flax epicotyls contains 22-24 copies of the t-dna encoded nopaline synthase gene per cell. all the gene copies are methylated to some extent but the methylation is not uniform, nor does it reflect the methylation level of the flanking plant dna. this extensive methylation correlates with an extremely low level of expression of the nopaline synthase gene. treatment of the tumor line with the in vivo demethylating drug 5-azacytidine at ... | 1983 | 6198416 |
| partial purification and properties of membrane-bound atpase in agrobacterium tumefaciens. | | 1982 | 6220646 |
| cloning and expression in escherichia coli of the tl-dna gene 4 of agrobacterium tumefaciens under the control of the pr promoter of bacteriophage lambda. | a plasmid was constructed that directs expression of the tl-dna gene 4 protein in e. coli. the different steps of the construction were as follows: i) a region of gene 4 encoding the amino-terminal portion of the protein was fused in frame to dna encoding an enzymatically active carboxy-terminal fragment of beta-galactosidase. the hybrid gene was poorly expressed from the upstream lambda pl promoter carried by the vector. ii) in order to generate an efficient procaryotic ribosome binding site, a ... | 1984 | 6241481 |
| physical mapping of part of the tumorigenic deoxyribonucleic acid (t-dan) region of the tumour-inducing (ti) plasmid from agrobacterium tumefaciens strain c58 [proceedings]. | | 1980 | 6245970 |
| cytochromes c-556 from three genetic races of agrobacterium. purification and comparison of their properties. | 1. the soluble cytochromes c-556 from three strains of agrobacterium tumefaciens, b6, ii chrys and apple 185 have been purified to homogeneity. the strains are representative members of the three main genetic races of agrobacterium. the purity of the final preparations was established by electrophoresis with an without sodium dodecyl sulphate, by analytical isoelectric focusing and ultracentrifugation, and by n-terminal analysis. 2. properties of these cytochromes were compared wih those of cyto ... | 1980 | 6249592 |
| tumor dna structure in plant cells transformed by a. tumefaciens. | crown gall tumors are induced in plants by infection with the soil bacterium agrobacterium tumefaciens. because the tumor induction involves transfer of a portion of the tumor-inducing (ti) plasmid dna from the bacterium to the plant cells, this system is of interest for the study of genetic exchange as well as tumor induction. the boundaries of the transferred dna (t-dna) have been cloned from transformed plant cells of tobacco. detailed mapping with restriction enzymes and nucleotide sequence ... | 1980 | 6251546 |
| characterization of tn904 insertions in octopine ti plasmid mutants of agrobacterium tumefaciens. | seven tn904 insertion mutants of pti ach5 affecting agrobacterium tumefaciens virulence were studied. the mutant character was shown to be plasmid borne. four of these mutants were avirulent and carried an insertion in restriction endonuclease hpai fragment 12, a 3.3-megadalton fragment, which therefore appears to be a ti plasmid region essential for virulence. two mutants were attenuated in virulence. the inserts mapped close to hpai fragment 12. one mutant giving rise to small tumors with exce ... | 1980 | 6252198 |
| is-like element is8 in rp4 plasmid and its involvement in cointegration. | the structure of the cointegrate plasmids formed by fusion of rp4 and the tumour-inducing plasmid (pti) of agrobacterium tumefaciens was analyzed. in all of the nine independently isolated pti::rp4 cointegrates, the integration occurred at the same site on the rp4 genome. moreover, a 1.2 md (1750 bp) rp4 sequence (is8) was directly repeated at both junction sites of the two replicons. the insertion of rp4 generated deletions, starting from the is8 sequence and extending into the ti part of the c ... | 1980 | 6253354 |
| agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. | mutants of agrobacterium tumefaciens which affect virulence or the ability to catabolize octopine were isolated after tn5-induced mutagenesis. of 8,900 colonies tested, 7 mutants with tn5 insertions in a specific region of other ti plasmid unable to catabolize octopine were isolated. thirty-seven mutants affected in tumorigenesis resulted from insertions in the ti plasmid and the agrobacterium chromosome. of these mutations, 12 were chromosomal and 25 mapped on the plasmid. twenty-three mapped w ... | 1980 | 6253441 |
| properties of six pesticide degradation plasmids isolated from alcaligenes paradoxus and alcaligenes eutrophus. | biophysical and genetic properties of six independently isolated plasmids encoding the degradation of the herbicides 2,4-dichlorophenoxyacetic acid and 4-chloro-2-methylphenoxyacetic acid are described. four of the plasmids, pjp3, pjp4, pjp5, and pjp7, had molecular masses of 51 megadaltons, belonged to the incp1 incompatibility group, and transferred freely to strains of escherichia coli, rhodopseudomonas sphaeroides, rhizobium sp., agrobacterium tumefaciens, pseudomonas putida, pseudomonas flu ... | 1981 | 6257648 |
| relationship between the limited and wide host range octopine-type ti plasmids of agrobacterium tumefaciens. | the relationship between the limited host range octopine ti plasmids and the wide host range octopine ti plasmids ptib6806 and ptia6 was studied. the limited host range ti plasmids shared extensive deoxyribonucleic acid homology; ptiag63 and ptiag162 were essentially completely homologous with ptiag158 while ptiag57 shared approximately 64% homology with ptiag158. in contrast, the limited host range octopine ti plasmids only shared 6 to 15% homology with the wide host range octopine ti plasmid p ... | 1981 | 6260751 |
| dna topoisomerase from agrobacterium tumefaciens: purification and catalytic properties. | the dna topoisomerase from agrobacterium tumefaciens has been purified to apparent homogeneity. the enzyme is a single polypeptide of about 100,000 in molecular weight. no apparent separation of the nicking and sealing activities could be obtained in attempts to separate the two activities by a variety of methods, including limited protease digestion, thermal denaturation, and differential inhibition. monoclonal antibodies obtained from hybridomas likewise did not preferentially inhibit one of t ... | 1981 | 6262720 |
| substituents at n6 and c-5' control selective uptake and toxicity of the adenine-nucleotide bacteriocin, agrocin 84, in agrobacteria. | the inhibition of a sensitive strain of agrobacterium radiobacter by the nucleotide bacteriocin agrocin 84 has been studied. a structure-function study of the agrocin 84 molecule was undertaken. two agrocin 84 nucleotide fragments lacking either the n6 or 5'-phosphoramidate substituents were used in uptake studies of [32p2]agrocin 84. it was established that the plasmid-controlled, strain-specific uptake of agrocin 84 is determined by the n6-d-glucofuranosyloxyphosphoramidate substituent. this c ... | 1981 | 6263633 |
| grown gall plant tumors of abnormal morphology, induced by agrobacterium tumefaciens carrying mutated octopine ti plasmids; analysis of t-dna functions. | ti plasmid mutants derived from agrobacterium tumefaciens strain ach5 that induce tumors of abnormal morphology have been analyzed. on tobacco, a. tumefaciens mutant strain lba4060 induces tumors that specifically give rise to shoots. shoots continue to grow from in vitro cultured bacteria-free tumor tissue derived from such tumors. the mutant character is shown to be correlated with the insertion of an a. tumefaciens is element, is60, into the left arm of the t-region of the octopine ti plasmid ... | 1981 | 6266929 |
| the functional organization of the octopine agrobacterium tumefaciens plasmid ptib6s3. | | 1981 | 6272338 |
| restriction endonuclease mapping of the octopine tumor-inducing plasmid ptiach5 of agrobacterium tumefaciens. | | 1981 | 6272339 |
| behavior of inc-q plasmids in agrobacterium tumefaciens. | | 1981 | 6273954 |
| physical mapping of dna base sequence homologies between an octopine and a nopaline ti plasmid of agrobacterium tumefaciens. | | 1981 | 6276566 |
| metal coordination centres of class ii cytochromes c. | the class ii cytochromes rhodospirillum molischianum cytochrome c', rhodopseudomonas palustris cytochrome c556 and agrobacterium tumefaciens (b2a) cytochrome c556 have been investigated with a variety of spectroscopic techniques. the cytochrome c' was found to be high-spin and the two cytochromes c556 were found to be mainly low-spin and sx-coordinate with the fifth and sixth ligands being histidine and methionine. the implications of the different types of iron coordination are discussed. | 1982 | 6279397 |
| construction and application of r prime plasmids, carrying different segments of an octopine ti plasmid from agrobacterium tumefaciens, for complementation of vir genes. | | 1982 | 6281831 |
| octopine ti-plasmid deletion mutants of agrobacterium tumefaciens with emphasis on the right side of the t-region. | | 1982 | 6283573 |
| genetic map of the crown gall suppressive incw plasmid psa. | a genetic map of the w incompatibility group plasmid psa has been prepared through the construction of deletion derivatives of psa and the cloning of various fragments of psa in pbr322. phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol, sulfonamides, spectinomycin, streptomycin, kanamycin, gentamycin, and tobramycin. information sufficient for the replication of the plasmid in both escherichia coli and agrobacterium tumefaciens i ... | 1982 | 6287164 |
| t-dna organization in homogeneous and heterogeneous octopine-type crown gall tissues of nicotiana tabacum. | octopine-type tumor tissue was obtained both by infection of plants or isolated protoplasts with agrobacterium tumefaciens and by somatic hybridization of normal and crown gall tobacco cells. analysis of t-dna by southern blotting of clones and uncloned tissue reveals that, whereas tumors induced on plants are heterogeneous mixtures of cells differing in t-dna organization, each tissue derived from transformed protoplasts or from somatic hybridization is homogeneous. detailed analysis of t-dna o ... | 1982 | 6291777 |
| agrobacterium tumefaciens mutants affected in attachment to plant cells. | an analysis of agrobacterium tumefaciens mutants with tn5 insertions in chromosomal dna showed that the chromosome of a. tumefaciens codes for a specific ability of this bacterium to attach to plant cells. this ability is associated with tumorigenesis by a. tumefaciens, the ability of avirulent a. tumefaciens to inhibit tumorigenesis, and the ability to adsorb certain phages. a second class of chromosomal mutations affects tumorigenesis without altering the ability to attach to plant cells. the ... | 1982 | 6292165 |
| mutational analysis of the virulence region of an agrobacterium tumefaciens ti plasmid. | forty-nine tn3 and tn5 transposition insertion mutations were introduced into the virulence region of the ptia6nc plasmid of agrobacterium tumefaciens. five tn5 transposition mutations from an earlier study (d. garfinkel and e. nester, j. bacteriol. 144:732-743, 1980) were also mapped more accurately. these mutations defined five separate loci within the virulence region. two tn3 insertions into one of these loci, vira, result in a strain which is only weakly virulent; however, a tn5 insertion i ... | 1983 | 6296058 |
| variation in hormone autonomy and regenerative potential of cells transformed by strain a66 of agrobacterium tumefaciens. | mutant agrobacterium tumefaciens strain a66 is shown to differ from its wild-type progenitor (strain a6) by a spontaneous 2.7 kb dna insert into the t-dna region of its ti plasmid. tobacco stems transformed by a66 exhibit an attenuated response characterized by slow growth and shoot proliferation. clonal analysis demonstrates that this response is due to an alteration in the growth and regenerative potential of transformed cells, rather than to variation in the frequency of fully autonomous cell ... | 1982 | 6297775 |
| the complete amino-acid sequence of the low-spin class ii cytochrome c-556 from agrobacterium tumefaciens strain b2a. | the amino acid sequence of the soluble monohaem cytochrome c-556 from agrobacterium tumefaciens, strain b2a, has been determined. the sequence was derived from peptides obtained by digestion of the apoprotein with trypsin and chymotrypsin, and by subdigestion of some of the peptides with staphylococcus aureus protease and thermolysin. sequencing of the various peptides was achieved by a combination of manual dansyl-edman degradation and automatic liquid-phase sequence analysis. the main characte ... | 1983 | 6297889 |
| transposon-facilitated chromosome mobilization in agrobacterium tumefaciens. | we improved chromosomal gene transfer in agrobacterium tumefaciens strain 15955 by constructing donors containing homologous transposons on both the sex factor plasmid and chromosome. first, we constructed plasmid pdp35, a kanamycin-sensitive derivative of r68.45. we then constructed derivatives of pdp35 that contained insertions of the kanamycin resistance transposon tn5. by restriction endonuclease analysis, we identified two plasmids, pdp37 and pdp38, in which tn5 was inserted in the same reg ... | 1983 | 6298186 |
| comparison of ti plasmids from three different biotypes of agrobacterium tumefaciens isolated from grapevines. | twenty-six plasmids from grapevine isolates of agrobacterium tumefaciens were analyzed by smai fingerprinting and by hybridization of nick-translated dna to dna of another plasmid. these experiments established that octopine ti plasmids are not highly conserved, although octopine ti plasmids from biotype 1 a. tumefaciens strains appeared to be very similar. octopine ti plasmids from biotype 3 strains are more variable in terms of host range and smai fingerprints, but share extensive dna homology ... | 1983 | 6298189 |