| newly identified pathogens associated with periodontitis: a systematic review. | there is substantial evidence supporting the role of certain oral bacteria species in the onset and progression of periodontitis. nevertheless, results of independent-culture diagnostic methods introduced about a decade ago have pointed to the existence of new periodontal pathogens. however, the data of these studies have not been evaluated together, which may generate some misunderstanding on the actual role of these microorganisms in the etiology of periodontitis. the aim of this systematic re ... | 2014 | 25074492 |
| kinetics of dithionite-dependent reduction of cytochrome p450 3a4: heterogeneity of the enzyme caused by its oligomerization. | to explore the basis of apparent conformational heterogeneity of cytochrome p450 3a4 (cyp3a4), the kinetics of dithionite-dependent reduction was studied in solution, in proteoliposomes, and in nanodiscs. in cyp3a4 oligomers in solution the kinetics obeys a three-exponential equation with similar amplitudes of each of the phases. addition of substrate (bromocriptine) displaces the phase distribution toward the slow phase at the expense of the fast one, while the middle phase remains unaffected. ... | 2005 | 16229479 |
| sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids. | the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ... | 2009 | 19857494 |
| sphingomyelin metabolism at the plasma membrane: implications for bioactive sphingolipids. | the plasma membrane (pm) is a major resource for production of bioactive lipids and contains a large proportion of the cellular sphingomyelin (sm) content. consequently, the regulation of sm levels at the pm by enzymes such as sphingomyelinase (smase) and sm synthase 2 (sms2) can have profound effects - both on biophysical properties of the membrane, but also on cellular signaling. over the past 20 years, there has been considerable research into the physiological and cellular functions associat ... | 2009 | 19857494 |
| biotechnological tools for environmental sustainability: prospects and challenges for environments in nigeria-a standard review. | the environment is a very important component necessary for the existence of both man and other biotic organisms. the degree of sustainability of the physical environment is an index of the survival and well-being of the entire components in it. additionally, it is not sufficient to try disposing toxic/deleterious substances with any known method. the best method of sustaining the environment is such that returns back all the components (wastes) in a recyclable way so that the waste becomes usef ... | 2012 | 22611499 |
| electronic waste - an emerging threat to the environment of urban india. | electronic waste or e-waste is one of the emerging problems in developed and developing countries worldwide. it comprises of a multitude of components with valuable materials, some containing toxic substances, that can have an adverse impact on human health and the environment. previous studies show that india has generated 0.4 million tons of e-waste in 2010 which may increase to 0.5 to 0.6 million tons by 2013-2014. coupled with lack of appropriate infrastructural facilities and procedures for ... | 2014 | 24444377 |
| the quest for a unified view of bacterial land colonization. | exploring molecular mechanisms underlying bacterial water-to-land transition represents a critical start toward a better understanding of the functioning and stability of the terrestrial ecosystems. here, we perform comprehensive analyses based on a large variety of bacteria by integrating taxonomic, phylogenetic and metagenomic data, in the quest for a unified view that elucidates genomic, evolutionary and ecological dynamics of the marine progenitors in adapting to nonaquatic environments. we ... | 2014 | 24451209 |
| a selective chromogenic plate, yeca, for the detection of pathogenic yersinia enterocolitica: specificity, sensitivity, and capacity to detect pathogenic y. enterocolitica from pig tonsils. | a new selective chromogenic plate, yeca, was tested for its specificity, sensitivity, and accuracy to detect pathogenic y. enterocolitica from pig tonsils. we tested a panel of 26 bacterial strains on yeca and compared it to pca, cin, and yecm media. detection of pathogenic y. enterocolitica was carried out on 50 pig tonsils collected in one slaughter house. enrichment was done in psb and itc broths. streaking on yeca and cin was done in direct, after 24h incubation of itc, after 48h incubation ... | 2011 | 22567328 |
| transcriptome analysis of campylobacter jejuni polyphosphate kinase (ppk1 and ppk2) mutants. | | 2015 | 26537695 |
| lack of negative effects on syrian hamsters and mongolian gerbils housed in the same secondary enclosure. | in cases where different species might be housed in the same room or secondary enclosure, the guide for the care and use of laboratory animals recommends that the animals should be behaviorally compatible and have the same health status. syrian hamsters and mongolian gerbils, both desert-dwelling rodents, appear to be reasonable candidates for such a combination. this study was undertaken to evaluate whether housing hamsters and gerbils in the same secondary enclosure is an acceptable practice. ... | 2015 | 26045450 |
| structural characterization of a 140 degrees domain movement in the two-step reaction catalyzed by 4-chlorobenzoate:coa ligase. | members of the adenylate-forming family of enzymes play a role in the metabolism of halogenated aromatics and of short, medium, and long chain fatty acids, as well as in the biosynthesis of menaquinone, peptide antibiotics, and peptide siderophores. this family includes a subfamily of acyl- and aryl-coa ligases that catalyze thioester synthesis through two half-reactions. a carboxylate substrate first reacts with atp to form an acyl-adenylate. subsequent to the release of the product pp i, the e ... | 2008 | 18620418 |
| comparative void-volume analysis of psychrophilic and mesophilic enzymes: structural bioinformatics of psychrophilic enzymes reveals sources of core flexibility. | psychrophiles, cold-adapted organisms, have adapted to live at low temperatures by using a variety of mechanisms. their enzymes are active at cold temperatures by being structurally more flexible than mesophilic enzymes. even though, there are some indications of the possible structural mechanisms by which psychrophilic enzymes are catalytic active at cold temperatures, there is not a generalized structural property common to all psychrophilic enzymes. | 2011 | 22013889 |
| pathology of spontaneous air sacculitis in 37 baboons and seven chimpanzees and a brief review of the literature. | air sacculitis is an important clinical condition in non-human primates. | 2012 | 22765381 |
| expression and characterization of thermotolerant lipase with broad ph profiles isolated from an antarctic pseudomonas sp strain ams3. | a gene encoding a thermotolerant lipase with broad ph was isolated from an antarctic pseudomonas strain ams3. the recombinant lipase ams3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. the molecular weight was approximately ∼60 kda including the strep and affinity tags. interestingly, the purified antarctic ams3 lipase exhibited broad temperature profile from 10-70 °c and stable over a broad ph ran ... | 2016 | 27781152 |
| cyanuric acid hydrolase from azorhizobium caulinodans ors 571: crystal structure and insights into a new class of ser-lys dyad proteins. | cyanuric acid hydrolase (cah) catalyzes the hydrolytic ring-opening of cyanuric acid (2,4,6-trihydroxy-1,3,5-triazine), an intermediate in s-triazine bacterial degradation and a by-product from disinfection with trichloroisocyanuric acid. in the present study, an x-ray crystal structure of the cah-barbituric acid inhibitor complex from azorhizobium caulinodans ors 571 has been determined at 2.7 å resolution. the cah protein fold consists of three structurally homologous domains forming a β-barre ... | 2014 | 24915109 |
| species sorting and neutral processes are both important during the initial assembly of bacterial communities. | many studies have shown that species sorting, that is, the selection by local environmental conditions is important for the composition and assembly of bacterial communities. on the other hand, there are other studies that could show that bacterial communities are neutrally assembled. in this study, we implemented a microcosm experiment with the aim to determine, at the same time, the importance of species sorting and neutral processes for bacterial community assembly during the colonisation of ... | 2011 | 21270841 |
| pyrosequencing-based analysis of the microbiome associated with the horn fly, haematobia irritans. | the horn fly, haematobia irritans, is one of the most economically important pests of cattle. insecticides have been a major element of horn fly management programs. growing concerns with insecticide resistance, insecticide residues on farm products, and non-availability of new generation insecticides, are serious issues for the livestock industry. alternative horn fly control methods offer the promise to decrease the use of insecticides and reduce the amount of insecticide residues on livestock ... | 2012 | 23028533 |
| bacterial communities associated with the surfaces of fresh fruits and vegetables. | fresh fruits and vegetables can harbor large and diverse populations of bacteria. however, most of the work on produce-associated bacteria has focused on a relatively small number of pathogenic bacteria and, as a result, we know far less about the overall diversity and composition of those bacterial communities found on produce and how the structure of these communities varies across produce types. moreover, we lack a comprehensive view of the potential effects of differing farming practices on ... | 2013 | 23544058 |
| parallel histories of horizontal gene transfer facilitated extreme reduction of endosymbiont genomes in sap-feeding insects. | bacteria confined to intracellular environments experience extensive genome reduction. in extreme cases, insect endosymbionts have evolved genomes that are so gene-poor that they blur the distinction between bacteria and endosymbiotically derived organelles such as mitochondria and plastids. to understand the host's role in this extreme gene loss, we analyzed gene content and expression in the nuclear genome of the psyllid pachypsylla venusta, a sap-feeding insect that harbors an ancient endosym ... | 2014 | 24398322 |
| protein domain architectures provide a fast, efficient and scalable alternative to sequence-based methods for comparative functional genomics. | a functional comparative genome analysis is essential to understand the mechanisms underlying bacterial evolution and adaptation. detection of functional orthologs using standard global sequence similarity methods faces several problems; the need for defining arbitrary acceptance thresholds for similarity and alignment length, lateral gene acquisition and the high computational cost for finding bi-directional best matches at a large scale. we investigated the use of protein domain architectures ... | 2016 | 27703668 |
| molecular modeling of family gh16 glycoside hydrolases: potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae. | family gh16 glycoside hydrolases can be assigned to five subgroups according to their substrate specificities, including xyloglucan transglucosylases/hydrolases (xths), (1,3)-beta-galactanases, (1,4)-beta-galactanases/kappa-carrageenases, "nonspecific" (1,3/1,3;1,4)-beta-d-glucan endohydrolases, and (1,3;1,4)-beta-d-glucan endohydrolases. a structured family gh16 glycoside hydrolase database has been constructed (http://www.ghdb.uni-stuttgart.de) and provides multiple sequence alignments with fu ... | 2004 | 15557263 |
| comparative genomics of regulation of heavy metal resistance in eubacteria. | heavy metal resistance (hmr) in eubacteria is regulated by a variety of systems including transcription factors from the merr family (cog0789). the hmr systems are characterized by the complex signal structure (strong palindrome within a 19 or 20 bp promoter spacer), and usually consist of transporter and regulator genes. some hmr regulons also include detoxification systems. the number of sequenced bacterial genomes is constantly increasing and even though hmr resistance regulons of the cog0789 ... | 2006 | 16753059 |
| a survey of orphan enzyme activities. | using computational database searches, we have demonstrated previously that no gene sequences could be found for at least 36% of enzyme activities that have been assigned an enzyme commission number. here we present a follow-up literature-based survey involving a statistically significant sample of such "orphan" activities. the survey was intended to determine whether sequences for these enzyme activities are truly unknown, or whether these sequences are absent from the public sequence databases ... | 2007 | 17623104 |
| microarray-based identification of differentially expressed genes in intracellular brucella abortus within raw264.7 cells. | brucella spp. is a species of facultative intracellular gram-negative bacteria that induces abortion and causes sterility in domesticated mammals and chronic undulant fever in humans. important determinants of brucella's virulence and potential for chronic infection include the ability to circumvent the host cell's internal surveillance system and the capability to proliferate within dedicated and non-dedicated phagocytes. hence, identifying genes necessary for intracellular survival may hold th ... | 2013 | 23950864 |
| reciprocal tripartite interactions between the aedes aegypti midgut microbiota, innate immune system and dengue virus influences vector competence. | dengue virus is one of the most important arboviral pathogens and the causative agent of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. it is transmitted between humans by the mosquitoes aedes aegypti and aedes albopictus, and at least 2.5 billion people are at daily risk of infection. during their lifecycle, mosquitoes are exposed to a variety of microbes, some of which are needed for their successful development into adulthood. however, recent studies have suggested that th ... | 2012 | 22413032 |
| overcoming differences: the catalytic mechanism of metallo-β-lactamases. | metallo-β-lactamases are the latest resistance mechanism of pathogenic and opportunistic bacteria against carbapenems, considered as last resort drugs. the worldwide spread of genes coding for these enzymes, together with the lack of a clinically useful inhibitor, have raised a sign of alarm. inhibitor design has been mostly impeded by the structural diversity of these enzymes. here we provide a critical review of mechanistic studies of the three known subclasses of metallo-β-lactamases, analyze ... | 2015 | 26297824 |
| a novel mva-mediated pathway for isoprene production in engineered e. coli. | to deal with the increasingly severe energy crisis and environmental consequences, biofuels and biochemicals generated from renewable resources could serve as a promising alternative for replacing petroleum as a source of fuel and chemicals, among which isoprene (2-methyl-1,3-butadiene) in particular is of great significance in that it is an important platform chemical, which has been used in industrial production of synthetic rubber for tires and coatings or aviation fuel. | 2016 | 26786050 |
| natural rice rhizospheric microbes suppress rice blast infections. | the natural interactions between plant roots and their rhizospheric microbiome are vital to plant fitness, modulating both growth promotion and disease suppression. in rice (oryza sativa), a globally important food crop, as much as 30% of yields are lost due to blast disease caused by fungal pathogen magnaporthe oryzae. capitalizing on the abilities of naturally occurring rice soil bacteria to reduce m. oryzae infections could provide a sustainable solution to reduce the amount of crops lost to ... | 2014 | 24884531 |
| ultrasound assessment of thrombotic complications in pediatric patients with tunneled central venous catheters. | central venous access consists in inserting a vascular catheter to the vena cava and placing its tip in the vicinity of the opening to the right atrium. in the patients of the clinic of pediatrics, hematology and oncology at the academic clinical centre of the medical university in gdańsk, such implantation procedures are conducted 40-50 times in a year using broviac/hickman catheters that are placed in the subclavian vein. in the ultrasound and biopsy laboratory at the clinic mentioned above, a ... | 2013 | 26673005 |
| comparative evaluation of the in-vitro activity of six β-lactam/β-lactamase inhibitor combinations against gram negative bacilli. | the extensive use of the β-lactam antibiotics in hospitals and in the community has created major resistance problems which has led to increased morbidity, mortality and healthcare costs. the use of the β-lactamase inhibitors in combination with the β-lactam antibiotics is currently the most successful strategy used for circumventing the resistance mechanisms. | 2013 | 23543071 |
| biochemistry and genetics of acc deaminase: a weapon to "stress ethylene" produced in plants. | 1-aminocyclopropane-1-carboxylate deaminase (accd), a pyridoxal phosphate-dependent enzyme, is widespread in diverse bacterial and fungal species. owing to accd activity, certain plant associated bacteria help plant to grow under biotic and abiotic stresses by decreasing the level of "stress ethylene" which is inhibitory to plant growth. accd breaks down acc, an immediate precursor of ethylene, to ammonia and α-ketobutyrate, which can be further metabolized by bacteria for their growth. acc deam ... | 2015 | 26441873 |
| inhibition of batrachochytrium dendrobatidis caused by bacteria isolated from the skin of boreal toads, anaxyrus (bufo) boreas boreas, from grand teton national park, wyoming, usa. | the chytrid fungus batrachochytrium dendrobatidis is a significant cause of the worldwide decline in amphibian populations; however, various amphibian species are capable of coexisting with b. dendrobatidis. among them are boreal toads (anaxyrus (bufo) boreas boreas) located in grand teton national park (gtnp) in wyoming, usa. the purpose of this study was to identify cultivable bacterial isolates from the skin microbiota of boreal toads from gtnp and determine if they were capable of inhibiting ... | 2014 | 24826077 |
| assessment of formate dehydrogenase stress stability in vivo using inactivation by hydrogen peroxide. | kinetic studies on hydrogen peroxide-induced inactivation of mutant formate dehydrogenase from pseudomonas sp. 101 (psefdh cys255ala) suggest a simple bimolecular mechanism for enzyme reaction with the inactivation agent. in the excess of hydrogen peroxide, the decrease in enzyme activity follows first-order kinetics. therefore, the first-order effective inactivation kinetic constants determined for various fdh forms at a constant h(2)o(2) concentration can be used as a quantitative measure of t ... | 2010 | 22649634 |
| glutamine versus ammonia utilization in the nad synthetase family. | nad is a ubiquitous and essential metabolic redox cofactor which also functions as a substrate in certain regulatory pathways. the last step of nad synthesis is the atp-dependent amidation of deamido-nad by nad synthetase (nads). members of the nads family are present in nearly all species across the three kingdoms of life. in eukaryotic nads, the core synthetase domain is fused with a nitrilase-like glutaminase domain supplying ammonia for the reaction. this two-domain nads arrangement enabling ... | 2012 | 22720044 |
| "hot cores" in proteins: comparative analysis of the apolar contact area in structures from hyper/thermophilic and mesophilic organisms. | a wide variety of stabilizing factors have been invoked so far to elucidate the structural basis of protein thermostability. these include, amongst the others, a higher number of ion-pairs interactions and hydrogen bonds, together with a better packing of hydrophobic residues. it has been frequently observed that packing of hydrophobic side chains is improved in hyperthermophilic proteins, when compared to their mesophilic counterparts. in this work, protein crystal structures from hyper/thermop ... | 2008 | 18312638 |
| crystal structure of the γ-hydroxymuconic semialdehyde dehydrogenase from pseudomonas sp. strainwbc-3, a key enzyme involved in para-nitrophenol degradation. | para-nitrophenol (pnp) is a highly toxic compound with threats to mammalian health. the pnpe-encoded γ-hydroxymuconic semialdehyde dehydrogenase catalyzes the reduction of γ-hydroxymuconic semialdehyde to maleylacetate in pseudomonas sp. strain wbc-3, playing a key role in the catabolism of pnp to krebs cycle intermediates. however, the catalyzing mechanism by pnpe has not been well understood. | 2013 | 24252642 |
| biosynthesis of rare hexoses using microorganisms and related enzymes. | rare sugars, referred to as monosaccharides and their derivatives that rarely exist in nature, can be applied in many areas ranging from foodstuffs to pharmaceutical and nutrition industry, or as starting materials for various natural products and drug candidates. unfortunately, an important factor restricting the utilization of rare sugars is their limited availability, resulting from limited synthetic methods. nowadays, microbial and enzymatic transformations have become a very powerful tool i ... | 2013 | 24367410 |
| silencing by h-ns potentiated the evolution of salmonella. | the bacterial h-ns protein silences expression from sequences with higher at-content than the host genome and is believed to buffer the fitness consequences associated with foreign gene acquisition. loss of h-ns results in severe growth defects in salmonella, but the underlying reasons were unclear. an experimental evolution approach was employed to determine which secondary mutations could compensate for the loss of h-ns in salmonella. six independently derived s. typhimurium hns mutant strains ... | 2014 | 25375226 |
| a new classification system for bacterial rieske non-heme iron aromatic ring-hydroxylating oxygenases. | rieske non-heme iron aromatic ring-hydroxylating oxygenases (rhos) are multi-component enzyme systems that are remarkably diverse in bacteria isolated from diverse habitats. since the first classification in 1990, there has been a need to devise a new classification scheme for these enzymes because many rhos have been discovered, which do not belong to any group in the previous classification. here, we present a scheme for classification of rhos reflecting new sequence information and interactio ... | 2008 | 18387195 |
| cholesterol oxidase: physiological functions. | an important aspect of catalysis performed by cholesterol oxidase (3beta-hydroxysteroid oxidase) concerns the nature of its association with the lipid bilayer that contains the sterol substrate. efficient catalytic turnover is affected by the association of the protein with the membrane as well as the solubility of the substrate in the lipid bilayer. in this review, the binding of cholesterol oxidase to the lipid bilayer, its turnover of substrates presented in different physical environments, a ... | 2009 | 19843168 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| functional gene diversity of soil microbial communities from five oil-contaminated fields in china. | to compare microbial functional diversity in different oil-contaminated fields and to know the effects of oil contaminant and environmental factors, soil samples were taken from typical oil-contaminated fields located in five geographic regions of china. geochip, a high-throughput functional gene array, was used to evaluate the microbial functional genes involved in contaminant degradation and in other major biogeochemical/metabolic processes. our results indicated that the overall microbial com ... | 2010 | 20861922 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |
| microbial degradation of petroleum hydrocarbon contaminants: an overview. | one of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. accidental releases of petroleum products are of particular concern in the environment. hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. ... | 2010 | 21350672 |
| genomic and functional analyses of the 2-aminophenol catabolic pathway and partial conversion of its substrate into picolinic acid in burkholderia xenovorans lb400. | 2-aminophenol (2-ap) is a toxic nitrogen-containing aromatic pollutant. burkholderia xenovorans lb400 possess an amn gene cluster that encodes the 2-ap catabolic pathway. in this report, the functionality of the 2-aminophenol pathway of b. xenovorans strain lb400 was analyzed. the amnrjbacdfehg cluster located at chromosome 1 encodes the enzymes for the degradation of 2-aminophenol. the absence of haba and habb genes in lb400 genome correlates with its no growth on nitrobenzene. rt-pcr analyses ... | 2013 | 24124510 |
| permanent draft genome sequence of comamonas testosteroni kf-1. | comamonas testosteroni kf-1 is a model organism for the elucidation of the novel biochemical degradation pathways for xenobiotic 4-sulfophenylcarboxylates (spc) formed during biodegradation of synthetic 4-sulfophenylalkane surfactants (linear alkylbenzenesulfonates, las) by bacterial communities. here we describe the features of this organism, together with the complete genome sequence and annotation. the 6,026,527 bp long chromosome (one sequencing gap) exhibits an average g+c content of 61.79% ... | 2013 | 23991256 |
| the catalytic roles of p185 and t188 and substrate-binding loop flexibility in 3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni. | 3α-hydroxysteroid dehydrogenase/carbonyl reductase from comamonas testosteroni reversibly catalyzes the oxidation of androsterone with nad(+) to form androstanedione and nadh. structurally the substrate-binding loop of the residues, t188-k208, is unresolved, while binding with nad(+) causes the appearance of t188-p191 in the binary complex. this study determines the functional roles of the flexible substrate-binding loop in conformational changes and enzyme catalysis. a stopped-flow study reveal ... | 2013 | 23717450 |
| identification of genes and pathways related to phenol degradation in metagenomic libraries from petroleum refinery wastewater. | two fosmid libraries, totaling 13,200 clones, were obtained from bioreactor sludge of petroleum refinery wastewater treatment system. the library screening based on pcr and biological activity assays revealed more than 400 positive clones for phenol degradation. from these, 100 clones were randomly selected for pyrosequencing in order to evaluate the genetic potential of the microorganisms present in wastewater treatment plant for biodegradation, focusing mainly on novel genes and pathways of ph ... | 2013 | 23637911 |
| infectious pulmonary disease in patients receiving immunosuppressive therapy for organ transplantation. | | 1999 | 10398512 |
| alginate lyase: review of major sources and classification, properties, structure-function analysis and applications. | alginate lyases catalyze the degradation of alginate, a complex copolymer of α-l-guluronate and its c5 epimer β-d-mannuronate. the enzymes have been isolated from various kinds of organisms with different substrate specificities, including algae, marine mollusks, marine and terrestrial bacteria, and some viruses and fungi. with the progress of structural biology, many kinds of alginate lyases of different polysaccharide lyases families have been characterized by obtaining crystal structures, and ... | 2015 | 25831216 |
| functional exploration of the polysaccharide lyase family pl6. | alginate, the main cell-wall polysaccharide of brown algae, is composed of two residues: mannuronic acid (m-residues) and, its c5-epimer, guluronic acid (g-residues). alginate lyases define a class of enzymes that cleave the glycosidic bond of alginate by β-elimination. they are classified according to their ability to recognize the distribution of m- and g-residues and are named m-, g- or mg-lyases. in the cazy database, alginate lyases have been grouped by sequence similarity into seven distin ... | 2016 | 27438604 |
| similar enzymes, different structures: phthalate dioxygenase is an alpha3alpha3 stacked hexamer, not an alpha3beta3 trimer like "normal" rieske oxygenases. | phthalate dioxygenase (pdo) is a member of a class of bacterial oxygenases that contain both rieske [2fe-2s] and fe(ii) mononuclear centers. recent crystal structures of several rieske dioxygenases showed that they exist as alpha(3)beta(3) multimers with subunits arranged head-to-tail in alpha and beta stacked planar rings. the structure of pdo, which consists of only alpha-subunits, remains to be solved. although similar to other rieske dioxygenases in many aspects, pdo was shown to differ in t ... | 2007 | 17764654 |
| finding intermediates in the o2 activation pathways of non-heme iron oxygenases. | intermediates in the reaction cycle of an oxygenase are usually very informative with respect to the chemical mechanism of o 2 activation and insertion. however, detection of these intermediates is often complicated by their short lifetime and the regulatory mechanism of the enzyme designed to ensure specificity. here, the methods used to detect the intermediates in an extradiol dioxygenase, a rieske cis-dihydrodiol dioxygenase, and soluble methane monooxygenase are discussed. the methods includ ... | 2007 | 17567087 |
| engineering non-heme mono- and dioxygenases for biocatalysis. | oxygenases are ubiquitous enzymes that catalyze the introduction of one or two oxygen atoms to unreactive chemical compounds. they require reduction equivalents from nadh or nadph and comprise metal ions, metal ion complexes, or coenzymes in their active site. thus, for industrial purposes, oxygenases are most commonly employed using whole cell catalysis, to alleviate the need for co-factor regeneration. biotechnological applications include bioremediation, chiral synthesis, biosensors, fine che ... | 2012 | 24688652 |
| prevent infection linked to the dialysis water in a hemodialysis center in fez city (morocco). | water treatment systems are a critical variable in dialysis therapy. rigorous control of hemodialysis water quality is particularly important in order to guarantee a better quality of life of the hemodialysis patients. the objective of the study was to evaluate the chemical, microbiological quality and antimicrobial resistance of bacteria isolated from water and dialysate in a public hd center. | 2013 | 24839530 |
| genome analysis and physiological comparison of alicycliphilus denitrificans strains bc and k601(t.). | the genomes of the betaproteobacteria alicycliphilus denitrificans strains bc and k601(t) have been sequenced to get insight into the physiology of the two strains. strain bc degrades benzene with chlorate as electron acceptor. the cyclohexanol-degrading denitrifying strain k601(t) is not able to use chlorate as electron acceptor, while strain bc cannot degrade cyclohexanol. the 16s rrna sequences of strains bc and k601(t) are identical and the fatty acid methyl ester patterns of the strains are ... | 2013 | 23825601 |
| efficient asymmetric synthesis of 1-cyano-tetrahydroisoquinolines from lipase dual activity and opposite enantioselectivities in α-aminonitrile resolution. | dual promiscuous racemization/amidation activities of lipases leading to efficient dynamic kinetic resolution protocols of racemic α-aminonitrile compounds are described. α-amidonitrile products of high enantiomeric purity could be formed in high yields. several lipases from different sources were shown to exhibit the dual catalytic activities, where opposite enantioselectivities could be recorded for certain substrates. | 2014 | 25055970 |
| two structurally different dienelactone hydrolases (tfdei and tfdeii) from cupriavidus necator jmp134 plasmid pjp4 catalyse cis- and trans-dienelactones with similar efficiency. | in this study, dienelactone hydrolases (tfdei and tfdeii) located on plasmid pjp4 of cupriavidus necator jmp134 were cloned, purified, characterized and three dimensional structures were predicted. tfdei and tfdeii genes were cloned into pet21b vector and expressed in e. coli bl21(de3). the enzymes were purified by applying ultra-membrane filtration, anion-exchange qff and gel-filtration columns. the enzyme activity was determined by using cis-dienelactone. the three-dimensional structure of enz ... | 2014 | 25054964 |
| rate of positive urine culture and double-j catheters colonization on the basis of microorganism dna analysis. | the aim of the trial was to estimate the relationship between colonization of the double-j catheter, and the microorganisms cultured from urine. | 2014 | 24982789 |
| burkholderia ambifaria and b. caribensis promote growth and increase yield in grain amaranth (amaranthus cruentus and a. hypochondriacus) by improving plant nitrogen uptake. | grain amaranth is an emerging crop that produces seeds having high quality protein with balanced amino-acid content. however, production is restricted by agronomic limitations that result in yields that are lower than those normally produced by cereals. in this work, the use of five different rhizobacteria were explored as a strategy to promote growth and yields in amaranthus hypochondriacus cv. nutrisol and a. cruentus cv. candil, two commercially important grain amaranth cultivars. the plants ... | 2014 | 24533068 |
| potential for plant growth promotion by a consortium of stress-tolerant 2,4-dinitrotoluene-degrading bacteria: isolation and characterization of a military soil. | the presence of explosives in soils and the interaction with drought stress and nutrient limitation are among the environmental factors that severely affect plant growth on military soils. in this study, we seek to isolate and identify the cultivable bacteria of a 2,4-dinitrotoluene (dnt) contaminated soil (ds) and an adjacent grassland soil (gs) of a military training area aiming to isolate new plant growth-promoting (pgp) and 2,4-dnt-degrading strains. metabolic profiling revealed disturbances ... | 2014 | 24467368 |
| a novel eurythermic and thermostale lipase lipm from pseudomonas moraviensis m9 and its application in the partial hydrolysis of algal oil. | lipases are regularly used in biotechnology to catalyse the hydrolysis of triglycerides and the synthesis of esters. microbial lipases in particular have been widely used in a variety of industrial applications. however, the current commercial microbial lipases cannot meet industrial demand due to rapid inactivation under harsh conditions. therefore, in order to identify more stable enzymes, we isolated novel eurythermic and thermostable lipase(s) from pseudomonas moraviensis m9. | 2015 | 26463643 |
| comparison of microbiomes from different niches of upper and lower airways in children and adolescents with cystic fibrosis. | changes in the airway microbiome may be important in the pathophysiology of chronic lung disease in patients with cystic fibrosis. however, little is known about the microbiome in early cystic fibrosis lung disease and the relationship between the microbiomes from different niches in the upper and lower airways. therefore, in this cross-sectional study, we examined the relationship between the microbiome in the upper (nose and throat) and lower (sputum) airways from children with cystic fibrosis ... | 2015 | 25629612 |
| deciphering a survival strategy during the interspecific competition between bacillus cereus msm-s1 and pseudomonas sp. msm-m1. | interspecific competition in bacteria governs colony growth dynamics and pattern formation. here, we demonstrate an interesting phenomenon of interspecific competition between bacillus cereus msm-s1 and pseudomonas sp. msm-m1, where secretion of an inhibitor by pseudomonas sp. is used as a strategy for survival. although b. cereus grows faster than pseudomonas sp., in the presence of pseudomonas sp. the population of b. cereus reduces significantly, whereas pseudomonas sp. do not show any marked ... | 2016 | 28018625 |
| isolation and identification of profenofos degrading bacteria. | an enrichment culture technique was used to isolate bacterial strains responsible for the biodegradation of profenofos in a soil from hubei province of central china. two pure bacterial cultures, named w and y, were isolated and subsequently characterized by sequencing of 16s rrna genes and biochemical tests. isolate w showed 96% similarity to the 16s rrna gene of a pseudomonas putida unlike y which showed 99% similarity to the 16s rrna gene of burkholderia gladioli. both strains grew well at ph ... | 2009 | 24031438 |
| structural and enzymatic characterization of the phosphotriesterase ophc2 from pseudomonas pseudoalcaligenes. | organophosphates (ops) are neurotoxic compounds for which current methods of elimination are unsatisfactory; thus bio-remediation is considered as a promising alternative. here we provide the structural and enzymatic characterization of the recently identified enzyme isolated from pseudomonas pseudoalcaligenes dubbed ophc2. ophc2 belongs to the metallo-β-lactamase superfamily and exhibits an unusual thermal resistance and some op degrading abilities. | 2013 | 24223749 |
| a treatment plant receiving waste water from multiple bulk drug manufacturers is a reservoir for highly multi-drug resistant integron-bearing bacteria. | the arenas and detailed mechanisms for transfer of antibiotic resistance genes between environmental bacteria and pathogens are largely unclear. selection pressures from antibiotics in situations where environmental bacteria and human pathogens meet are expected to increase the risks for such gene transfer events. we hypothesize that waste-water treatment plants (wwtps) serving antibiotic manufacturing industries may provide such spawning grounds, given the high bacterial densities present there ... | 2013 | 24204801 |
| computational design of a ph stable enzyme: understanding molecular mechanism of penicillin acylase's adaptation to alkaline conditions. | protein stability provides advantageous development of novel properties and can be crucial in affording tolerance to mutations that introduce functionally preferential phenotypes. consequently, understanding the determining factors for protein stability is important for the study of structure-function relationship and design of novel protein functions. thermal stability has been extensively studied in connection with practical application of biocatalysts. however, little work has been done to ex ... | 2014 | 24959852 |
| evaluation of chiral alpha-cyanoesters as general fluorescent substrates for screening enantioselective esterases. | esterases play a crucial role in industrial chemical synthesis, maintaining normal physiological metabolism and detoxifying exogenous ester-containing toxicants. to meet the rapidly increasing industrial need for all kinds of esterases, especially enantioselective esterases used to generate highly pure chiral compounds, general substrates are necessary for rapid screening, monitoring, purification, and characterization. in this study, general fluorescent substrates including phenolic derivatives ... | 2006 | 16448170 |
| versatility of biological non-heme fe(ii) centers in oxygen activation reactions. | oxidase and oxygenase enzymes allow the use of relatively unreactive o2 in biochemical reactions. many of the mechanistic strategies used in nature for this key reaction are represented within the 2-histidine-1-carboxylate facial triad family of non-heme fe(ii)-containing enzymes. the open face of the metal coordination sphere opposite the three endogenous ligands participates directly in the reaction chemistry. here, data from several studies are presented showing that reductive o2 activation w ... | 2008 | 18277980 |
| cloning and characterisation of (r)-3-hydroxyacyl-acyl carrier protein-coenzyme a transferase gene (phag) from pseudomonas sp. usm 4-55. | the (r)-3-hydroxyacyl-acp-coa transferase catalyses the conversion of (r)-3-hydroxyacyl-acp to (r)-3-hydroxyacyl-coa derivatives, which serves as the ultimate precursor for polyhydroxyalkanoate (pha) polymerisation from unrelated substrates in pseudomonads. phag was found to be responsible for channelling precursors for polyhydroxyalkanoate (pha) synthase from a de novo fatty acid biosynthesis pathway when cultured on carbohydrates, such as glucose or gluconate. the phag gene was cloned from pse ... | 2009 | 24575175 |
| caenorhabditis elegans genomic response to soil bacteria predicts environment-specific genetic effects on life history traits. | with the post-genomic era came a dramatic increase in high-throughput technologies, of which transcriptional profiling by microarrays was one of the most popular. one application of this technology is to identify genes that are differentially expressed in response to different environmental conditions. these experiments are constructed under the assumption that the differentially expressed genes are functionally important in the environment where they are induced. however, whether differential e ... | 2009 | 19503598 |
| the pha depolymerase engineering database: a systematic analysis tool for the diverse family of polyhydroxyalkanoate (pha) depolymerases. | polyhydroxyalkanoates (phas) can be degraded by many microorganisms using intra- or extracellular pha depolymerases. pha depolymerases are very diverse in sequence and substrate specificity, but share a common alpha/beta-hydrolase fold and a catalytic triad, which is also found in other alpha/beta-hydrolases. | 2009 | 19296857 |
| rhamnolipid biosurfactants as new players in animal and plant defense against microbes. | rhamnolipids are known as very efficient biosurfactant molecules. they are used in a wide range of industrial applications including food, cosmetics, pharmaceutical formulations and bioremediation of pollutants. the present review provides an overview of the effect of rhamnolipids in animal and plant defense responses. we describe the current knowledge on the stimulation of plant and animal immunity by these molecules, as well as on their direct antimicrobial properties. given their ecological a ... | 2010 | 21614194 |
| whole-cell fluorescent biosensors for bioavailability and biodegradation of polychlorinated biphenyls. | whole-cell microbial biosensors are one of the newest molecular tools used in environmental monitoring. such biosensors are constructed through fusing a reporter gene such as lux, gfp or lacz, to a responsive promoter. there have been many reports of the applications of biosensors, particularly their use in assaying pollutant toxicity and bioavailability. this paper reviews the basic concepts behind the construction of whole-cell microbial biosensors for pollutant monitoring, and describes the a ... | 2010 | 22205873 |
| hydrolytic potential of a psychrotrophic pseudomonas isolated from refrigerated raw milk. | the production of extracellular hydrolases by a psychrotrophic bacterium isolated from refrigerated raw milk, and identified as a pseudomonas sp. belonging to the pseudomonas jenssenii group, was studied. this bacterium produced proteolytic and lipolytic enzymes in all media investigated (skim milk, cheese whey, casein broth, and tryptone soy broth). high levels of α-glucosidase were produced in skim milk broth. hydrolytic enzymes detected in skim milk broth are of particular concern, indicating ... | 2011 | 24031781 |
| characterization of bacteria associated with pinewood nematode bursaphelenchus xylophilus. | pine wilt disease (pwd) is a complex disease integrating three major agents: the pathogenic agent, the pinewood nematode bursaphelenchus xylophilus; the insect-vector monochamus spp.; and the host pine tree, pinus sp. since the early 80's, the notion that another pathogenic agent, namely bacteria, may play a role in pwd has been gaining traction, however the role of bacteria in pwd is still unknown. the present work supports the possibility that some b. xylophilus-associated bacteria may play a ... | 2012 | 23091599 |
| evaluation and biochemical characterization of a distinctive pyoverdin from a pseudomonas isolated from chickpea rhizosphere. | microbial siderophores confiscate the available ferric ions around the roots and trigger a reaction resulting in plant growth promotion. in our study, a high level of siderophore production was observed from a newly isolated pseudomonas sp. from the rhizosphere of chickpea plants. under an iron depleted condition in standard succinic acid medium a 1000 μgml(-1) of siderophore production was achieved. increasing the concentration of iron showed an inverse relationship between growth and sideropho ... | 2012 | 24031875 |
| plant-growth promoting effect of newly isolated rhizobacteria varies between two arabidopsis ecotypes. | various rhizobacteria are known for their beneficial effects on plants, i. e. promotion of growth and induction of systemic resistance against pathogens. these bacteria are categorized as plant growth promoting rhizobacteria (pgpr) and are associated with plant roots. knowledge of the underlying mechanisms of plant growth promotion in vivo is still very limited, but interference of bacteria with plant hormone metabolism is suggested to play a major role. to obtain new growth promoting bacteria, ... | 2012 | 22580689 |
| investigation of the biosynthetic potential of endophytes in traditional chinese anticancer herbs. | traditional chinese medicine encompasses a rich empirical knowledge of the use of plants for the treatment of disease. in addition, the microorganisms associated with medicinal plants are also of interest as the producers of the compounds responsible for the observed plant bioactivity. the present study has pioneered the use of genetic screening to assess the potential of endophytes to synthesize bioactive compounds, as indicated by the presence of non-ribosomal peptide synthetase (nrps) and pol ... | 2012 | 22629306 |
| convergent development of anodic bacterial communities in microbial fuel cells. | microbial fuel cells (mfcs) are often inoculated from a single wastewater source. the extent that the inoculum affects community development or power production is unknown. the stable anodic microbial communities in mfcs were examined using three inocula: a wastewater treatment plant sample known to produce consistent power densities, a second wastewater treatment plant sample, and an anaerobic bog sediment. the bog-inoculated mfcs initially produced higher power densities than the wastewater-in ... | 2012 | 22572637 |
| evolution of rep diversity: a comparative study. | repetitive extragenic palindromic elements (reps) constitute a group of bacterial genomic repeats known for their high abundance and several roles in host cells´ physiology. we analyzed the phylogenetic distribution of particular rep classes in genomic sequences of sixty-three bacterial strains belonging to the pseudomonas fluorescens species complex and ten strains of stenotrophomonas sp., in order to assess intraspecific rep diversity and to gain insight into long-term rep evolution. | 2013 | 23758774 |
| halomonas sp. bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in india and their biomedical importance. | halophilic bacteria were isolated from thamaraikulam solar salt works in india. after routine biosurfactant screening by various methods, the biosurfactant producing bacteria, halomonas sp bs4 was confirmed by 16 s rrna sequencing. the growth optimization of halomonas sp bs4 revealed their optimum growth at 8% nacl and 6-8 ph in the growth medium. further the partially purified biosurfactants were characterized by tlc, ftir and gc-ms analysis. gc-ms results revealed that, the partial purified bi ... | 2013 | 23667807 |
| biosurfactants in agriculture. | agricultural productivity to meet growing demands of human population is a matter of great concern for all countries. use of green compounds to achieve the sustainable agriculture is the present necessity. this review highlights the enormous use of harsh surfactants in agricultural soil and agrochemical industries. biosurfactants which are reported to be produced by bacteria, yeasts, and fungi can serve as green surfactants. biosurfactants are considered to be less toxic and eco-friendly and thu ... | 2013 | 23280539 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| soil salinity: a serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. | salinity is one of the most brutal environmental factors limiting the productivity of crop plants because most of the crop plants are sensitive to salinity caused by high concentrations of salts in the soil, and the area of land affected by it is increasing day by day. for all important crops, average yields are only a fraction - somewhere between 20% and 50% of record yields; these losses are mostly due to drought and high soil salinity, environmental conditions which will worsen in many region ... | 2014 | 25737642 |
| p. aeruginosa sgnh hydrolase-like proteins algj and algx have similar topology but separate and distinct roles in alginate acetylation. | the o-acetylation of polysaccharides is a common modification used by pathogenic organisms to protect against external forces. pseudomonas aeruginosa secretes the anionic, o-acetylated exopolysaccharide alginate during chronic infection in the lungs of cystic fibrosis patients to form the major constituent of a protective biofilm matrix. four proteins have been implicated in the o-acetylation of alginate, algijf and algx. to probe the biological function of algj, we determined its structure to 1 ... | 2014 | 25165982 |
| biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity. | current soil management strategies are mainly dependent on inorganic chemical-based fertilizers, which caused a serious threat to human health and environment. the exploitation of beneficial microbes as a biofertilizer has become paramount importance in agriculture sector for their potential role in food safety and sustainable crop production. the eco-friendly approaches inspire a wide range of application of plant growth promoting rhizobacteria (pgprs), endo- and ectomycorrhizal fungi, cyanobac ... | 2014 | 24885352 |
| bacterial degradation of chlorophenols and their derivatives. | chlorophenols (cps) and their derivatives are persistent environmental pollutants which are used in the manufacture of dyes, drugs, pesticides and other industrial products. cps, which include monochlorophenols, polychlorophenols, chloronitrophenols, chloroaminophenols and chloromethylphenols, are highly toxic to living beings due to their carcinogenic, mutagenic and cytotoxic properties. several physico-chemical and biological methods have been used for removal of cps from the environment. bact ... | 2014 | 24589366 |
| comparative genomics of pseudomonas fluorescens subclade iii strains from human lungs. | while the taxonomy and genomics of environmental strains from the p. fluorescens species-complex has been reported, little is known about p. fluorescens strains from clinical samples. in this report, we provide the first genomic analysis of p. fluorescens strains in which human vs. environmental isolates are compared. | 2015 | 26644001 |
| composition of the spruce budworm (choristoneura fumiferana) midgut microbiota as affected by rearing conditions. | the eastern spruce budworm (choristoneura fumiferana) is one of the most destructive forest insect pests in canada. little is known about its intestinal microbiota, which could play a role in digestion, immune protection, communication and/or development. the present study was designed to provide a first characterization of the effects of rearing conditions on the taxonomic diversity and structure of the c. fumiferana midgut microbiota, using a culture-independent approach. three diets and insec ... | 2015 | 26636571 |
| a fragrant neighborhood: volatile mediated bacterial interactions in soil. | there is increasing evidence that volatile organic compounds (vocs) play essential roles in communication and competition between soil microorganisms. here we assessed volatile-mediated interactions of a synthetic microbial community in a model system that mimics the natural conditions in the heterogeneous soil environment along the rhizosphere. phylogenetic different soil bacterial isolates (burkholderia sp., dyella sp., janthinobacterium sp., pseudomonas sp., and paenibacillus sp.) were inocul ... | 2015 | 26579111 |
| phylogenetic analysis based evolutionary study of 16s rrna in known pseudomonas sp. | molecular evolution analysis of 16s rrna sequences of native pseudomonas strains and different fluorescent pseudomonads were conducted on the basis of molecular evolutionary genetics analysis version 5.2 (mega5.2). topological evaluations show common origin for native strains with other known strains with available sequences at genbank database. phylogenetic affiliation of different pseudomonas sp based on 16s rrna gene shows that molecular divergence contributes to the genetic diversity of pseu ... | 2015 | 26664032 |
| biodegradation kinetics and interactions of styrene and ethylbenzene as single and dual substrates for a mixed bacterial culture. | this study examined biodegradation kinetics of styrene and ethylbenzene as representatives of alkenylbenzenes and mono-alkylbenzenes, respectively. the compounds were studied independently and as binary mixtures using a series of aerobic batch degradation experiments introduced by acclimatized mix culture. initial concentration of styrene and ethylbenzene in the liquid phase vacillated from 0 to 220 mg/l. the andrew model was applied for the biodegradation of individual substrates and the estima ... | 2015 | 26483955 |
| the genomic landscape of ribosomal peptides containing thiazole and oxazole heterocycles. | ribosomally synthesized and post-translationally modified peptides (ripps) are a burgeoning class of natural products with diverse activity that share a similar origin and common features in their biosynthetic pathways. the precursor peptides of these natural products are ribosomally produced, upon which a combination of modification enzymes installs diverse functional groups. this genetically encoded peptide-based strategy allows for rapid diversification of these natural products by mutation i ... | 2015 | 26462797 |
| fiber-optic chemical sensors and fiber-optic bio-sensors. | this review summarizes principles and current stage of development of fiber-optic chemical sensors (focs) and biosensors (fobs). fiber optic sensor (fos) systems use the ability of optical fibers (of) to guide the light in the spectral range from ultraviolet (uv) (180 nm) up to middle infrared (ir) (10 μm) and modulation of guided light by the parameters of the surrounding environment of the of core. the introduction of of in the sensor systems has brought advantages such as measurement in flamm ... | 2015 | 26437407 |
| an automated genomes-to-natural products platform (gnp) for the discovery of modular natural products. | bacterial natural products are a diverse and valuable group of small molecules, and genome sequencing indicates that the vast majority remain undiscovered. the prediction of natural product structures from biosynthetic assembly lines can facilitate their discovery, but highly automated, accurate, and integrated systems are required to mine the broad spectrum of sequenced bacterial genomes. here we present a genome-guided natural products discovery tool to automatically predict, combinatorialize ... | 2015 | 26412281 |
| immobilization of microbes for bioremediation of crude oil polluted environments: a mini review. | petroleum hydrocarbons are the most common environmental pollutants in the world and oil spills pose a great hazard to terrestrial and marine ecosystems. oil pollution may arise either accidentally or operationally whenever oil is produced, transported, stored and processed or used at sea or on land. oil spills are a major menace to the environment as they severely damage the surrounding ecosystems. to improve the survival and retention of the bioremediation agents in the contaminated sites, bac ... | 2015 | 26668662 |
| unearthing the genomes of plant-beneficial pseudomonas model strains wcs358, wcs374 and wcs417. | plant growth-promoting rhizobacteria (pgpr) can protect plants against pathogenic microbes through a diversity of mechanisms including competition for nutrients, production of antibiotics, and stimulation of the host immune system, a phenomenon called induced systemic resistance (isr). in the past 30 years, the pseudomonas spp. pgpr strains wcs358, wcs374 and wcs417 of the willie commelin scholten (wcs) collection have been studied in detail in pioneering papers on the molecular basis of pgpr-me ... | 2015 | 26198432 |
| bacteria isolated from bats inhibit the growth of pseudogymnoascus destructans, the causative agent of white-nose syndrome. | emerging infectious diseases are a key threat to wildlife. several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. white-nose syndrome, caused by the fungal skin pathogen pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. the skin microbiome is increasingly understood to play a large role in determini ... | 2015 | 25853558 |