| description of shewanella glacialipiscicola sp. nov. and shewanella algidipiscicola sp. nov., isolated from marine fish of the danish baltic sea, and proposal that shewanella affinis is a later heterotypic synonym of shewanella colwelliana. | two novel species belonging to the genus shewanella are described on the basis of a polyphasic taxonomic approach. a total of 40 strains of gram-negative, psychrotolerant, h2s-producing bacteria were isolated from marine fish (cod and plaice) caught in the baltic sea off denmark. strains belonging to group 1 (seven strains) were a lactate-assimilating variant of shewanella morhuae with a g+c content of 44 mol%. the strains of group 2 (33 strains) utilized lactate, n-acetylglucosamine and malate ... | 2007 | 17267977 |
| proteomic profiling of intact proteins using wax-rplc 2-d separations and fticr mass spectrometry. | we investigated the combination of weak anion exchange (wax) fractionation and on-line reversed-phase liquid chromatography (rplc) separation using a 12 t fticr mass spectrometer for the detection of intact proteins from a shewanella oneidensis mr-1 cell lysate. this work aimed at optimizing intact protein detection for profiling proteins at a level that incorporates their modification state. a total of 715 intact proteins were detected, and the combined results from the wax fractions and the un ... | 2007 | 17269717 |
| profiling the membrane proteome of shewanella oneidensis mr-1 with new affinity labeling probes. | the membrane proteome plays a critical role in electron transport processes in shewanella oneidensis mr-1, a bacterial organism that has great potential for bioremediation. biotinylation of intact cells with subsequent affinity-enrichment has become a useful tool for characterization of the membrane proteome. as opposed to these commonly used, water-soluble commercial reagents, we here introduce a family of hydrophobic, cell-permeable affinity probes for extensive labeling and detection of membr ... | 2007 | 17269728 |
| influence of calcium on microbial reduction of solid phase uranium(vi). | the effect of calcium on the dissolution and microbial reduction of a representative solid phase uranyl [u(vi)], sodium boltwoodite (nauo(2)sio(3)oh . 1.5h(2)o), was investigated to evaluate the rate-limiting step of microbial reduction of the solid phase u(vi). microbial reduction experiments were performed in a culture of a dissimilatory metal-reducing bacterium (dmrb), shewanella oneidensis strain mr-1, in a bicarbonate medium with lactate as electron donor at ph 6.8 buffered with pipes. calc ... | 2007 | 17274063 |
| charge-associated effects of fullerene derivatives on microbial structural integrity and central metabolism. | the effects of four types of fullerene compounds (c60, c60-oh, c60-cooh, c60-nh2) were examined on two model microorganisms (escherichia coli w3110 and shewanella oneidensis mr-1). positively charged c60-nh2 at concentrations as low as 10 mg/l inhibited growth and reduced substrate uptake for both microorganisms. scanning electron microscopy (sem) revealed damage to cellular structures. neutrally charged c60 and c60-oh had mild negative effects on s. oneidensis mr-1, whereas the negatively charg ... | 2007 | 17288489 |
| [anaerobic humus respiration by shewanella cinica d14t]. | experimental results suggested shewanella cinica d14t is capable of humus respiration utilizing various organic acids and some important environmental pollutants (e.g., toluene. etc) as electron donors and aqs or aqds as a sole terminal electron acceptor under anaerobic condition. the dissimilatory reduction of 1mmol/l aqds can couple to the production of enough atp to support cell growth about 60 generations; the oxidization of electron donors was coupled to the reduction of humus, as reduced h ... | 2006 | 17302164 |
| [isolation and characterization of two aniline-degrading strains and compare of functional genes]. | two aniline-degrading bacterial strains, an30 and dn425, were isolated from activated sludge of textile-printing wastewater treatment plant and identified as pseudomonas sp. and shewanella sp. , respectively. under shaking condition, 250 mg/l aniline was removed 96.1% and 13.8% within 72h by strains an30 and dn425, respectively. under static condition, their degrading rates were 39.6% and 8.6% , respectively. under static condition, the decolorizing rate of strain dn425 reached 96% for azo dye g ... | 2006 | 17304852 |
| plant carbohydrate scavenging through tonb-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. | tonb-dependent receptors (tbdrs) are outer membrane proteins mainly known for the active transport of iron siderophore complexes in gram-negative bacteria. analysis of the genome of the phytopathogenic bacterium xanthomonas campestris pv. campestris (xcc), predicts 72 tbdrs. such an overrepresentation is common in xanthomonas species but is limited to only a small number of bacteria. here, we show that one xcc tbdr transports sucrose with a very high affinity, suggesting that it might be a sucro ... | 2007 | 17311090 |
| remodeling of the bacterial rna polymerase supramolecular complex in response to environmental conditions. | directed binding of rna polymerase to distinct promoter elements controls transcription and promotes adaptive responses to changing environmental conditions. to identify proteins that modulate transcription, we have expressed a tagged alpha-subunit of rna polymerase in shewanella oneidensis under controlled growth conditions, isolated the protein complex using newly developed multiuse affinity probes, and used lc-ms/ms to identify proteins in the complex. complementary fluorescence correlation s ... | 2007 | 17319694 |
| shewanella infection in decompensated liver disease: a septic case. | shewanella species are an unusual cause of disease in humans. however, reports of shewanella infections have been increasing, and hepatobiliary disease has been proposed as a predisposing factor following a critical course. we report the first japanese septic case of decompensated liver disease in which this bacterium acted as a definite pathogen. a 67-year-old japanese man with primary sclerosing cholangitis was admitted to our hospital complaining of fever, general fatigue, pain, and a rash on ... | 2007 | 17322999 |
| competitive binding of cu2+ and zn2+ to live cells of shewanella putrefaciens. | binding of cu2+ and zn2+ to live cells of shewanella putrefaciens was measured at ph 4, 5.5, and 7 for dissolved metal concentrations ranging from 0.1 to 100 microm. release of organic compounds by the cells resulted in concentrations of dissolved organic carbon (doc) between 0.5 and 1.6 mm. a discrete site, nonelectrostatic model was used to describe cu2+ and zn2+ binding to the cells. binding of zn2+, which increased with increasing ph over the entire range of dissolved zn2+ concentration, cou ... | 2007 | 17328202 |
| regeneration of iron for trichloroethylene reduction by shewanella alga bry. | zero valent iron (zvi), the primary reactive material in several permeable reactive barriers, is often oxidized to ferrous or ferric iron, resulting in decreased reactivity with time. iron reducing bacteria can reconvert the ferric iron to its ferrous form, prolonging the reduction of chlorinated organic contaminants. in this study, the reduction of fe(ii,iii) oxide and fe(iii) oxide by a strain of iron reducing bacteria of the group shewanella alga bry(s. alga bry) was observed in both aqueous ... | 2007 | 17349671 |
| functional roles of the heme architecture and its environment in tetraheme cytochrome c. | cytochromes are involved in a wide variety of redox reactions in living systems. some of them contain multiple hemes such as desulfovibrio cytochrome c3 and shewanella small tetraheme cytochrome c. the significance of c-type tetraheme architectures was discussed. a cyclic heme architecture and its environment regulate the extremely low redox potentials of cytochrome c3 in addition to bis-imidazole coordination and heme exposure. each heme in cytochrome c3 plays a different role in the electron t ... | 2007 | 17370988 |
| dosage-dependent proteome response of shewanella oneidensis mr-1 to acute chromate challenge. | proteome alterations in the metal-reducing bacterium shewanella oneidensis mr-1 in response to different acute dose challenges (0.3, 0.5, or 1 mm) of the toxic metal chromate [cr(vi)] were characterized with multidimensional hplc-ms/ms. proteome measurements were performed and compared on both quadrupole ion traps as well as linear trapping quadrupole mass spectrometers. we have found that the implementation of multidimensional liquid chromatography on-line with the rapid scanning, high throughp ... | 2007 | 17385904 |
| mathematical model for predicting microbial reduction and transport of arsenic in groundwater systems. | a mathematical model was developed for describing the transport of arsenic, coupled with microbially-mediated biogeochemical processes. the biogeochemical characteristics of arsenic reactive transport processes were investigated in both batch and column tests, which showed that as(v) was reduced to as(iii) by shewanella sp., with the reduced arsenic species subsequently removed by precipitation. the breakthrough data obtained from the column experiments were used for the calibration of the arsen ... | 2007 | 17399762 |
| metabolic activity and phylogenetic diversity of reed (phragmites australis) periphyton bacterial communities in a hungarian shallow soda lake. | in the present study, the species composition and potential metabolic activities of bacterial communities of reed phragmites australis (cav.) (trin. ex steudel) periphyton from lake velencei were studied by cultivation-based and metabolic fingerprinting methods. serially diluted spring biofilm samples were used to test the community-level physiological profiling (clpp) using biolog microplates, and for plating onto different media. on the basis of their morphological, biochemical, and physiologi ... | 2007 | 17406774 |
| effects of temperature and dissolved oxygen on se(iv) removal and se(0) precipitation by shewanella sp. hn-41. | facultative anaerobic shewanella sp. strain hn-41 was able to utilize selenite (se(iv)) as a sole electron acceptor for respiration in anaerobic condition, resulting in reduction of se(iv) and then precipitation of elemental se nano-sized spherical particles, which were identified using energy-dispersive x-ray spectroscopy and x-ray absorption near-edge structure spectroscopy. when the effects on se(iv) reduction to elemental se were studied by varying incubation temperatures and dissolved oxyge ... | 2007 | 17434567 |
| kinetic analysis of microbial reduction of fe(iii) in nontronite. | microbial reduction of structural fe(iii) in nontronite (nau-2) was studied in batch cultures under non-growth condition using shewanella putrefaciens strain cn32. the rate and extent of structural fe(iii) reduction was examined as a function of electron acceptor [fe(iii)] and bacterial concentration. fe(ll) sorption onto nau-2 and cn32 surfaces was independently measured and described by the langmuir expression with the affinity constant (log k) of 3.21 and 3.30 for nau-2 and bacteria, respecti ... | 2007 | 17438797 |
| sigma 28 promoter prediction in members of the gammaproteobacteria. | a two-step approach was used to predict sigma(28) promoter sequences in a number of gammaproteobacteria. putative promoters were initially identified upstream of motility and chemotaxis genes in test species based on their similarity to the escherichia coli sigma(28) promoter consensus. these sequences were then used to generate position specific score matrices that were used iteratively during whole-genome analyses. the consistent identification of promoter sequences upstream of orthologous gen ... | 2007 | 17439543 |
| shewanella spp. infection following treatment for upper gastrointestinal bleeding. | shewanella spp. are an uncommon cause of human infection, with exposure to water being the commonest source. we report a patient with a malignancy and upper gastrointestinal bleeding who underwent a gastric lavage followed by an endoscopy as part of her investigations. she subsequently developed shewanella spp. bacteraemia without any clinical source of infection. | 2007 | 17454904 |
| characterisation of the bacterial flora of modified atmosphere packaged farmed atlantic cod (gadus morhua) by pcr-dgge of conserved 16s rrna gene regions. | the present article describes the use of broad-range molecular analyses to characterise the microbial population of farmed atlantic cod (gadus morhua) packaged for the retail market. cod was filleted post rigor, packaged in air or in modified atmosphere (ma) (50% co(2):50% n(2) or 50% co(2):50% o(2)) and stored at 0 degrees c for 11 days. to determine the community profiles of the samples the variable v3-region of the bacterial 16s rrna gene were amplified by pcr, before the pcr products were se ... | 2007 | 17467836 |
| specific bonds between an iron oxide surface and outer membrane cytochromes mtrc and omca from shewanella oneidensis mr-1. | shewanella oneidensis mr-1 is purported to express outer membrane cytochromes (e.g., mtrc and omca) that transfer electrons directly to fe(iii) in a mineral during anaerobic respiration. a prerequisite for this type of reaction would be the formation of a stable bond between a cytochrome and an iron oxide surface. atomic force microscopy (afm) was used to detect whether a specific bond forms between a hematite (fe(2)o(3)) thin film, created with oxygen plasma-assisted molecular beam epitaxy, and ... | 2007 | 17468239 |
| diversity and enzyme properties of protease-producing bacteria isolated from sub-antarctic sediments of isla de los estados, argentina. | protease-producing bacteria isolated from sub-antarctic marine sediments of isla de los estados (argentina) were characterized, and the thermal inactivation kinetics of their extracellular proteases compared. isolates were affiliated with the genera pseudoalteromonas, shewanella, colwellia, planococcus, and a strain to the family flavobacteriaceae. colwellia strains were moderate psychrophiles (optimal growth at about 15 degrees c, maximum growth temperature at around 25 degrees c). 16s rrna phy ... | 2007 | 17487446 |
| immunoaffinity purification and characterization of rna polymerase from shewanella oneidensis. | shewanella oneidensis is of particular interest for research because of its unique ability to use a variety of metals as final respiratory electron acceptors and reduce them into insoluble oxides. a collection of monoclonal antibodies (mabs) that were prepared towards escherichia coli rna polymerase (rnap) was tested for reactivity with proteins extracted from s. oneidensis. two polyol-responsive monoclonal antibodies (pr-mabs) were used to purify rna polymerase from s. oneidensis using immunoaf ... | 2007 | 17507238 |
| construction of a low-temperature protein expression system using a cold-adapted bacterium, shewanella sp. strain ac10, as the host. | a recombinant protein expression system working at low temperatures is expected to be useful for the production of thermolabile proteins. we constructed a low-temperature expression system using an antarctic cold-adapted bacterium, shewanella sp. strain ac10, as the host. we evaluated the promoters for proteins abundantly produced at 4 degrees c in this bacterium to express foreign proteins. we used 27 promoters and a broad-host-range vector, pjrd215, to produce beta-lactamase in shewanella sp. ... | 2007 | 17526788 |
| the surface physicochemistry and adhesiveness of shewanella are affected by their surface polysaccharides. | shewanella strains have previously been studied with regard to their cell surface ultrastructure and lps composition. they have now been further characterized with respect to their surface physicochemistry and ability to adhere to haematite. the surfaces of the shewanella strains were found to be electronegative and hydrophilic, and these properties could be correlated with lps composition or the presence of capsular polysaccharides. strains expressing rough lps with no capsule were more hydroph ... | 2007 | 17526844 |
| targeted tandem mass spectrometry for high-throughput comparative proteomics employing nanolc-fticr ms with external ion dissociation. | targeted tandem mass spectrometry (ms/ms) is an attractive proteomic approach that allows selective identification of peptides exhibiting abundance differences, e.g., between culture conditions and/or diseased states. herein, we report on a targeted lc-ms/ms capability realized with a hybrid quadrupole-7 tesla fourier transform ion cyclotron resonance (fticr) mass spectrometer that provides data-dependent ion selection, accumulation, and dissociation external to the icr trap, and a control softw ... | 2007 | 17531500 |
| biological reduction of np(v) and np(v) citrate by metal-reducing bacteria. | oxidized actinide species are often more mobile than reduced forms. bioremediation strategies have been developed to exploit this chemistry and stabilize actinides in subsurface environments. we investigated the ability of metal-reducing bacteria geobacter metallireducens and shewanella oneidensis to enzymatically reduce np(v) and np(v) citrate, as well as the toxicity of np(v) to these organisms. a toxic effect was observed for both bacteria at concentrations of > or = 4.0 mm np(v) citrate. bel ... | 2007 | 17533836 |
| structural, thermodynamic, and mutational analyses of a psychrotrophic rnase hi. | ribonuclease (rnase) hi from the psychrotrophic bacterium shewanella oneidensis mr-1 was overproduced in escherichia coli, purified, and structurally and biochemically characterized. the amino acid sequence of mr-1 rnase hi is 67% identical to that of e. coli rnase hi. the crystal structure of mr-1 rnase hi determined at 2.0 a resolution was highly similar to that of e. coli rnase hi, except that the number of intramolecular ion pairs and the fraction of polar surface area of mr-1 rnase hi were ... | 2007 | 17536836 |
| cell-free synthesis and maturation of [fefe] hydrogenases. | [fefe] hydrogenases catalyze the reversible reduction of protons to molecular hydrogen (adams (1990); biochim biophys acta 1020(2): 115-145) and are of significant interest for the biological production of hydrogen fuel. they are complex proteins with active sites containing iron, sulfur, and carbon monoxide and cyanide ligands (peters et al. (1998); science 282(5395): 1853-1858). maturation enzymes for [fefe] hydrogenases have been identified (posewitz et al. (2004); j biol chem 279(24): 25711- ... | 2008 | 17546685 |
| the three-dimensional crystal structure of the prpf protein of shewanella oneidensis complexed with trans-aconitate: insights into its biological function. | in bacteria, the dehydration of 2-methylcitrate to yield 2-methylaconitate in the 2-methylcitric acid cycle is catalyzed by a cofactor-less (prpd) enzyme or by an aconitase-like (acnd) enzyme. bacteria that use acnd also require the function of the prpf protein, whose function was previously unknown. to gain insights into the function of prpf, the three-dimensional crystal structure of the prpf protein from the bacterium shewanella oneidensis was solved at 2.0 a resolution. the protein fold of p ... | 2007 | 17567742 |
| influence of lipopolysaccharide on the surface proton-binding behavior of shewanella spp. | this study investigates the potentiometric properties of several strains of shewanella spp. and determines whether these properties can be correlated with lipopolysaccharide (lps) type. the lps of eight shewanella strains was characterized using silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and their potentiometric properties determined using high-resolution acid-base titrations. titrations showed that total ligand concentrations (l(t)) ranged from 0.903 +/- ... | 2007 | 17570012 |
| extracellular respiration. | although it has long been known that microbes can generate energy using diverse strategies, only recently has it become clear that a growing number involve electron transfer to or from extracellular substrates. the best-known example of what we will term 'extracellular respiration' is electron transfer between microbes and minerals, such as iron and manganese (hydr)oxides. this makes sense, given that these minerals are sparingly soluble. what is perhaps surprising, however, is that a number of ... | 2007 | 17581115 |
| respiration of metal (hydr)oxides by shewanella and geobacter: a key role for multihaem c-type cytochromes. | dissimilatory reduction of metal (e.g. fe, mn) (hydr)oxides represents a challenge for microorganisms, as their cell envelopes are impermeable to metal (hydr)oxides that are poorly soluble in water. to overcome this physical barrier, the gram-negative bacteria shewanella oneidensis mr-1 and geobacter sulfurreducens have developed electron transfer (et) strategies that require multihaem c-type cytochromes (c-cyts). in s. oneidensis mr-1, multihaem c-cyts cyma and mtra are believed to transfer ele ... | 2007 | 17581116 |
| [taxonomic composition of bacteria associated with cultivated mollusks crassostrea lugubris and perna viridis and with the water of the gulf of nha trang lagoon, vietnam]. | one hundred and four strains of heterotrophic bacteria have been isolated and characterized from two species of bivalve mollusks cultivated in the gulf of nha trang (vietnam) and from the water of a mariculture farm. the isolates have been identified on the basis of morphological, physiological, biochemical, and chemotaxonomic properties, as well as by the content of g+c bases in dna. in the microflora of mollusks, vibrio alginolyticus was predominant; the pathogenic species v. harveyi and v. sp ... | 2007 | 17583223 |
| biodegradation of textile azo dye by shewanella decolorationis s12 under microaerophilic conditions. | the complete biodegradation of azo dye, fast acid red gr, was observed under microaerophilic conditions by shewanella decolorationis s12. although the highest decolorizing rate was measured under anaerobic condition and the highest biomass was obtained under aerobic condition, a further biodegradation of decolorizing products can only be achieved under microaerophilic conditions. under microaerophilic conditions, s. decolorationis s12 could use a range of carbon sources for azo dye decolorizatio ... | 2007 | 17589840 |
| identification and analysis of a highly conserved chemotaxis gene cluster in shewanella species. | a conserved cluster of chemotaxis genes was identified from the genome sequences of fifteen shewanella species. an in-frame deletion of the chea-3 gene, which is located in this cluster, was created in s. oneidensis mr-1 and the gene shown to be essential for chemotactic responses to anaerobic electron acceptors. the chea-3 protein showed strong similarity to vibrio cholerae chea-2 and p. aeruginosa chea-1, two proteins that are also essential for chemotaxis. the genes encoding these proteins we ... | 2007 | 17590227 |
| hydrologic flow controls on biologic iron(iii) reduction in natural sediments. | bacterial reduction of a hematite-rich natural coastal sand was studied in flow-through column reactors at flow rates which varied from 0.62 to 11 pore volumes d(-1). sand columns were wet-packed with the dissimilatory metal-reducing bacterium (dmrb) shewanella putrefaciens cn32, and a pipes-buffered, lactate-containing growth medium was pumped through the columns for over 20 days. soluble fe(ii), acetate and lactate concentrations measured in the column effluents showed that steady-state condit ... | 2007 | 17593722 |
| diversifying biological fuel cell designs by use of nanoporous filters. | the use of proton exchange membranes (pems) in biological fuel cells limits the diversity of novel designs for increasing output power or enabling autonomous function in unique environments. here we show that selected nanoporous polymer filters (nylon, cellulose, or polycarbonate) can be used effectively in place of pems in a miniature microbial fuel cell (mini-mfc, device cross-section 2 cm2), generating a power density of 16 w/m3 with an uncoated graphite felt oxygen reduction reaction (orr) c ... | 2007 | 17593755 |
| identification of the gene encoding a type 1 rnase h with an n-terminal double-stranded rna binding domain from a psychrotrophic bacterium. | the gene encoding a bacterial type 1 rnase h, termed rbd-rnase hi, was cloned from the psychrotrophic bacterium shewanella sp. sib1, overproduced in escherichia coli, and the recombinant protein was purified and biochemically characterized. sib1 rbd-rnase hi consists of 262 amino acid residues and shows amino acid sequence identities of 26% to sib1 rnase hi, 17% to e. coli rnase hi, and 32% to human rnase h1. sib1 rbd-rnase hi has a double-stranded rna binding domain (rbd) at the n-terminus, whi ... | 2007 | 17608717 |
| a kinetic approach to the dependence of dissimilatory metal reduction by shewanella oneidensis mr-1 on the outer membrane cytochromes c omca and omcb. | the gram-negative bacterium shewanella oneidensis mr-1 shows a remarkably versatile anaerobic respiratory metabolism. one of its hallmarks is its ability to grow and survive through the reduction of metallic compounds. among other proteins, outer membrane decaheme cytochromes c omca and omcb have been identified as key players in metal reduction. in fact, both of these cytochromes have been proposed to be terminal fe(iii) and mn(iv) reductases, although their role in the reduction of other metal ... | 2007 | 17608722 |
| pathway of phytate dephosphorylation by beta-propeller phytases of different origins. | using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the beta-propeller phytase of shewanella oneidensis was established, which was then compared with that of bacillus subtilis 168, bacillus amyloliquefaciens atcc 15841, and b. amyloliquefaciens 45 beta-propeller phytases. the data demonstrate that all of these beta-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospec ... | 2007 | 17612603 |
| quality attributes of farmed eel (anguilla anguilla) stored under air, vacuum and modified atmosphere packaging at 0 degrees c. | the shelf life of fresh eel in various packaging conditions of atmospheric air, vacuum and modified atmosphere packaging (map) (40% co(2), 30% n(2) and 30% o(2)) at 0 degrees c was investigated. all raw eel samples received acceptable sensory scores during the first 11+/-1 days of storage in atmospheric air, 11+/-1 days of storage in vacuum and finally 18+/-1 days of storage in map conditions. using the microbial quality indicators the shelf life of eel packed in air, vacuum and map was estimate ... | 2007 | 17613370 |
| proteomic studies of an antarctic cold-adapted bacterium, shewanella livingstonensis ac10, for global identification of cold-inducible proteins. | proteomic analysis of a cold-adapted bacterium, shewanella livingstonensis ac10, isolated from antarctic seawater was carried out to elucidate its cold-adaptation mechanism. the cells were grown at 4 degrees c and 18 degrees c, and soluble and membrane proteins were analyzed by two-dimensional gel electrophoresis. at 4 degrees c, the relative abundance of 47 soluble proteins and five membrane proteins increased more than twofold, and these proteins were analyzed by peptide mass fingerprinting. t ... | 2007 | 17618403 |
| paraferrimonas sedimenticola gen. nov., sp. nov., a marine bacterium of the family ferrimonadaceae. | three strains (mok-106(t), mok-142 and mok-143) were isolated from marine sediment samples collected from the coast of okinawa island, japan. on the basis of 16s rrna gene sequence comparisons, the isolates were affiliated with the family ferrimonadaceae; shewanella denitrificans and ferrimonas balearica were the closest relatives, having sequence similarities of 93.7 and 93.0 %, respectively. the novel isolates shared high levels of 16s rrna gene sequence similarity with each other (98.7-99.3 % ... | 2007 | 17625182 |
| direct determination of oxidation state of gold deposits in metal-reducing bacterium shewanella algae using x-ray absorption near-edge structure spectroscopy (xanes). | x-ray absorption near-edge structure spectroscopy (xanes) was successfully employed to determine the gold valence in the metal-reducing bacterium shewanella algae after exposure to a 1 mm aqueous haucl4 solution for 10-120 min. xanes spectra revealed the oxidation state of gold in the bacterial cells to be au(0) without any contribution from au(iii), demonstrating that s. algae cells can reduce aucl4- ions to elemental gold. transmission electron microscopy (tem) and energy dispersive x-ray (edx ... | 2007 | 17630130 |
| phylogenetic and physiological diversity of dissimilatory ferric iron reducers in sediments of the polluted scheldt estuary, northwest europe. | the potential for dissimilatory ferric iron [fe(iii)] reduction in intertidal sediments of the polluted scheldt estuary, northwest europe, was assessed by combining field-based geochemical measurements with laboratory experiments on the associated microbiology. microbial communities at a freshwater and brackish location were characterized by culture-independent 16s rrna gene analysis, as well as enrichments, strain isolation and physiological screening. dilution-to-extinction batch enrichments u ... | 2007 | 17635542 |
| current production and metal oxide reduction by shewanella oneidensis mr-1 wild type and mutants. | shewanella oneidensis mr-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. s. oneidensis mr-1 can reduce mn(iv) and fe(iii) oxides and can produce current in microbial fuel cells. the mechanisms that are employed by s. oneidensis mr-1 to execute these processes have not yet been fully elucidated. several different s. oneidensis mr-1 deletion mutants were generated and tested for current production and metal oxi ... | 2007 | 17644630 |
| plutonium(iv) reduction by the metal-reducing bacteria geobacter metallireducens gs15 and shewanella oneidensis mr1. | the bacterial reduction of actinides has been suggested as a possible remedial strategy for actinide-contaminated environments, and the bacterial reduction of pu(vi/v) has the potential to produce highly insoluble pu(iv) solid phases. however, the behavior of plutonium with regard to bacterial reduction is more complex than for other actinides because it is possible for pu(iv) to be further reduced to pu(iii), which is relatively more soluble than pu(iv). this work investigates the ability of th ... | 2007 | 17644643 |
| crystal structures of two novel dye-decolorizing peroxidases reveal a beta-barrel fold with a conserved heme-binding motif. | btdyp from bacteroides thetaiotaomicron (strain vpi-5482) and tyra from shewanella oneidensis are dye-decolorizing peroxidases (dyps), members of a new family of heme-dependent peroxidases recently identified in fungi and bacteria. here, we report the crystal structures of btdyp and tyra at 1.6 and 2.7 a, respectively. btdyp assembles into a hexamer, while tyra assembles into a dimer; the dimerization interface is conserved between the two proteins. each monomer exhibits a two-domain, alpha+beta ... | 2007 | 17654545 |
| identification and structural characterization of heme binding in a novel dye-decolorizing peroxidase, tyra. | tyra is a member of the dye-decolorizing peroxidase (dyp) family, a new family of heme-dependent peroxidase recently identified in fungi and bacteria. here, we report the crystal structure of tyra in complex with iron protoporphyrin (ix) at 2.3 a. tyra is a dimer, with each monomer exhibiting a two-domain, alpha/beta ferredoxin-like fold. both domains contribute to the heme-binding site. co-crystallization in the presence of an excess of iron protoporphyrin (ix) chloride allowed for the unambigu ... | 2007 | 17654547 |
| vascular steal syndrome and shewanella alga infection requiring amputation in a hemodialysis patient. | shewanella alga is a rare gram-negative marine bacterium. its role as a pathogenic organism is gradually evolving with sporadic cases being reported in humans. we report a case of vascular steal syndrome secondary to a polytetrafluoroethylene (ptfe) graft in the upper extremity of an end-stage renal disease (esrd) patient on maintenance hemodialysis, which was complicated by infection with s. alga. this resulted in extensive myonecrosis requiring amputation of the limb. to our knowledge this is ... | 2003 | 17657124 |
| roles of a short connecting disulfide bond in the stability and function of psychrophilic shewanella violacea cytochrome c (5). | cys-59 and cys-62, forming a disulfide bond in the four-residue loop of shewanella violacea cytochrome c (5) (sv cytc (5)), contribute to protein stability but not to redox function. these cys residues were substituted with ala in sv cytc (5), and the structural and functional properties of the resulting c59a/c62a variant were determined and compared with those of the wild-type. the variant had similar features to those of the wild-type in absorption, circular dichroic, and paramagnetic (1)h nmr ... | 2007 | 17657404 |
| an octaheme c-type cytochrome from shewanella oneidensis can reduce nitrite and hydroxylamine. | a c-type cytochrome from shewanella oneidensis mr-1, containing eight hemes, has been previously designated as an octaheme tetrathionate reductase (otr). the structure of otr revealed that the active site contains an unusual lysine-ligated heme, despite the presence of a cxxch motif in the sequence that would predict histidine ligation. this lysine ligation has been previously observed only in the pentaheme nitrite reductases, suggesting that otr may have a possible role in nitrite reduction. we ... | 2007 | 17659281 |
| a novel filamentous phage from the deep-sea bacterium shewanella piezotolerans wp3 is induced at low temperature. | active filamentous phage particles were isolated from the deep-sea bacterium shewanella piezotolerans wp3. a putative single-stranded dna binding protein of the phage was found to be overexpressed at 4 degrees c compared to its expression at 25 degrees c by two-dimensional polyacrylamide gel electrophoresis. reverse transcription quantitative pcr further revealed that the key genes of the sw1 phage were significantly induced at low temperature. | 2007 | 17660281 |
| diverse tetracycline resistant bacteria and resistance genes from coastal waters of jiaozhou bay. | environmental microbiology investigation was carried out in jiaozhou bay to determine the source and distribution of tetracycline-resistant bacteria and their resistance mechanisms. at least 25 species or the equivalent molecular phylogenetic taxa in 16 genera of resistant bacteria could be identified based on 16s ribosomal deoxyribonucleic acid sequence analysis. enterobacteriaceae, pseudomonadaceae, and vibrionaceae constituted the majority of the typical resistant isolates. indigenous estuari ... | 2008 | 17668262 |
| characterization of protein-protein interactions involved in iron reduction by shewanella oneidensis mr-1. | the interaction of proteins implicated in dissimilatory metal reduction by shewanella oneidensis mr-1 (outer membrane [om] proteins omca, mtrb, and mtrc; om-associated protein mtra; periplasmic protein ccta; and cytoplasmic membrane protein cyma) were characterized by protein purification, analytical ultracentrifugation, and cross-linking methods. five of these proteins are heme proteins, omca (83 kda), mtrc (75 kda), mtra (32 kda), ccta (19 kda), and cyma (21 kda), and can be visualized after s ... | 2007 | 17675441 |
| informatics strategies for large-scale novel cross-linking analysis. | the detection of protein interactions in biological systems represents a significant challenge for today's technology. chemical cross-linking provides the potential to impart new chemical bonds in a complex system that result in mass changes in a set of tryptic peptides detected by mass spectrometry. however, system complexity and cross-linking product heterogeneity have precluded widespread chemical cross-linking use for large-scale identification of protein-protein interactions. the developmen ... | 2007 | 17676784 |
| a general computational model for predicting ribosomal frameshifts in genome sequences. | programmed ribosomal frameshifts are frequently used by rna viruses to synthesize a single fusion protein from two or more overlapping open reading frames. we previously developed a program called fsfinder for predicting -1 and +1 frameshifts for windows systems. with our new web application and web service called fsfinder2, users can predict frameshifts of any type from any web browser and operating system. we tested it on shewanella oneidensis mr-1, for which exact frameshift sites are not kno ... | 2007 | 17678640 |
| expression in periplasmic space of shewanella oneidensis. | a shewanella expression system has been used for an overproduction of c-type multiheme proteins. the proteins were exported to the periplasmic space for the maturation. since the periplasmic expression system is attractive, especially for protease-sensitive proteins, an expression vector containing a signal peptide was constructed for expressions in the periplasmic space of shewanella oneidensis. to evaluate the system, two eukaryotic proteins which originally do not have signal sequences and ar ... | 2007 | 17689099 |
| whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation. | while bacterial genome annotations have significantly improved in recent years, techniques for bacterial proteome annotation (including post-translational chemical modifications, signal peptides, proteolytic events, etc.) are still in their infancy. at the same time, the number of sequenced bacterial genomes is rising sharply, far outpacing our ability to validate the predicted genes, let alone annotate bacterial proteomes. in this study, we use tandem mass spectrometry (ms/ms) to annotate the p ... | 2007 | 17690205 |
| isolation and characterization of bacteria from the copepod pseudocaligus fugu ectoparasitic on the panther puffer takifugu pardalis with the emphasis on ttx. | a total of 50 bacterial isolates was obtained from the copepod pseudocaligus fugu, which is a common parasite, collected from the body surface of the panther puffer takifugu pardalis. on the basis of colony characteristics, these bacterial isolates were grouped into six types, of which only two (types-i and -ii) showed a high affinity for adhesion to the carapace of the banana shrimp penaeus merguiensis. these two types of adhesive bacteria were identified through 16s rrna sequence analysis as s ... | 2007 | 17698158 |
| characterization of shewanella oneidensis mtrc: a cell-surface decaheme cytochrome involved in respiratory electron transport to extracellular electron acceptors. | mtrc is a decaheme c-type cytochrome associated with the outer cell membrane of fe(iii)-respiring species of the shewanella genus. it is proposed to play a role in anaerobic respiration by mediating electron transfer to extracellular mineral oxides that can serve as terminal electron acceptors. the present work presents the first spectropotentiometric and voltammetric characterization of mtrc, using protein purified from shewanella oneidensis mr-1. potentiometric titrations, monitored by uv-vis ... | 2007 | 17701062 |
| the antioxidative function of eicosapentaenoic acid in a marine bacterium, shewanella marinintestina ik-1. | when the eicosapentaenoic acid (epa)-deficient mutant strain ik-1delta8 of the marine epa-producing shewanella marinintestina ik-1 was treated with various concentrations of hydrogen peroxide (h(2)o(2)), its colony-forming ability decreased more than that of the wild type. protein carbonylation, induced by treating cells with 0.01 mm h(2)o(2) under bacteriostatic conditions, was enhanced only in cells lacking epa. the amount of cells recovered from the cultures was decreased more significantly b ... | 2007 | 17706200 |
| diversity of shewanella population in fish sparus aurata harvested in the aegean sea. | to study the diversity of shewanella population in sparus aurata fish harvested in the aegean sea, as well as to elucidate the influence of fish storage conditions on the selection in shewanella strains. | 2007 | 17714405 |
| antimicrobial properties of moderately halotolerant bacteria from cenotes of the yucatan peninsula. | isolation and antimicrobial evaluation of aquatic bacterial strains from two cenotes. | 2007 | 17718841 |
| activation of year-yoag operon transcription by the nitrate-responsive regulator narl is independent of oxygen- responsive regulator fnr in escherichia coli k-12. | the facultative aerobe escherichia coli k-12 can use respiratory nitrate ammonification to generate energy during anaerobic growth. the toxic compound nitric oxide is a by-product of this metabolism. previous transcript microarray studies identified the year-yoag operon, encoding proteins of unknown function, among genes whose transcription is induced in response to nitrate, nitrite, or nitric oxide. nitrate and nitrite regulate anaerobic respiratory gene expression through the narx-narl and nar ... | 2007 | 17720788 |
| metal reduction kinetics in shewanella. | metal reduction kinetics have been studied in cultures of dissimilatory metal reducing bacteria which include the shewanella oneidensis strain mr-1. estimation of system parameters from time-series data faces obstructions in the implementation depending on the choice of the mathematical model that captures the observed dynamics. the modeling of metal reduction is often based on michaelis-menten equations. these models are often developed using initial in vitro reaction rates and seldom match wit ... | 2007 | 17720985 |
| identification of a new hemolysin from diarrheal isolate ssu of aeromonas hydrophila. | a clinical strain ssu of aeromonas hydrophila produces a potent cytotoxic enterotoxin (act) with cytotoxic, enterotoxic, and hemolytic activities. a new gene, which encoded a hemolysin of 439-amino acid residues with a molecular mass of 49 kda, was identified. this hemolysin (hlya) was detected based on the observation that the act gene minus mutant of a. hydrophila ssu still had residual hemolytic activity. the new hemolysin gene (hlya) was cloned, sequenced, and overexpressed in escherichia co ... | 2007 | 17725618 |
| shewanella putrefaciens produces an fe(iii)-solubilizing organic ligand during anaerobic respiration on insoluble fe(iii) oxides. | the mechanism of fe(iii) reduction was investigated using voltammetric techniques in anaerobic incubations of shewanella putrefaciens strain 200 supplemented with fe(iii) citrate or a suite of fe(iii) oxides as terminal electron acceptor. results indicate that organic complexes of fe(iii) are produced during the reduction of fe(iii) at rates that correlate with the reactivity of the fe(iii) phase and bacterial cell density. anaerobic fe(iii) solubilization activity is detected with either fe(iii ... | 2007 | 17765315 |
| shewanella canadensis sp. nov. and shewanella atlantica sp. nov., manganese dioxide- and hexahydro-1,3,5-trinitro-1,3,5-triazine-reducing, psychrophilic marine bacteria. | two strains belonging to the genus shewanella, haw-eb2(t) and haw-eb5(t), were isolated previously from marine sediment sampled from the atlantic ocean, near halifax harbour in canada, for their potential to degrade explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx). in the present study, strains haw-eb2(t) and haw-eb5(t) were found to display high 16s rrna gene sequence similarity (90-99.5 %) to species of shewanella, but their gyrb sequences were significantly different from each other an ... | 2007 | 17766891 |
| nucleotide and deduced amino acid sequences of a subtilisin-like serine protease from a deep-sea bacterium, alkalimonas collagenimarina ac40(t). | the acpi gene encoding an alkaline protease (acpi) from a deep-sea bacterium, alkalimonas collagenimarina ac40(t), was shotgun-cloned and sequenced. it had a 1,617-bp open reading frame encoding a protein of 538 amino acids. based on analysis of the deduced amino acid sequence, acpi is a subtilisin-like serine protease belonging to subtilase family a. it consists of a prepropeptide, a catalytic domain, and a prepeptidase c-terminal domain like other serine proteases from the genera pseudomonas, ... | 2007 | 17786425 |
| mediation of sulfur speciation by a black sea facultative anaerobe. | shewanella putrefaciens, a respiratory facultative anaerobe isolated from the black sea, can reduce thiosulfate, sulfite, and elemental sulfur to sulfide readily and quantitatively. this widespread and anaerobically versatile microorganism, which is incapable of reducing sulfate, uses oxidized sulfur intermediates as electron acceptors during the respiratory oxidation of organic matter. because of its widespread distribution and abundance, it may play a significant role in sulfur and trace metal ... | 1993 | 17809344 |
| effect of natural organic matter on zinc inhibition of hematite bioreduction by shewanella putrefaciens cn32. | the effect of zinc on the biological reduction of hematite (alpha-fe2o3) by the dissimilatory metal-reducing bacterium (dmrb) shewanella putrefaciens cn32 was studied in the presence of four natural organic materials (noms). experiments were performed under non-growth conditions with h2 as the electron donor and zinc inhibition was quantified as the decrease in the 5 d extent of hematite bioreduction as compared to no-zinc controls. every nom was shown to significantly increase zinc inhibition d ... | 2007 | 17822092 |
| current production by a deep-sea strain shewanella sp. ds1. | electricity-generating bacteria have attracted intensive studies in recent years as an important resource for energy generation. in this investigation, a bacterial isolate capable of producing electric current during fermentation processes was obtained from a deep-sea hydrothermal field in the atlantic ocean for the first time. the strain, assigned to shewanella sp. ds1 based on its 16s-rrna sequence analysis, grew at the optimum temperature of 30 degrees c and optimum ph of 6.5. the results sho ... | 2007 | 17828572 |
| prospecting the proteome: identification of naturally occurring binding motifs for biarsenical probes. | | 2007 | 17828727 |
| orthologous transcription factors in bacteria have different functions and regulate different genes. | transcription factors (tfs) form large paralogous gene families and have complex evolutionary histories. here, we ask whether putative orthologs of tfs, from bidirectional best blast hits (bbhs), are evolutionary orthologs with conserved functions. we show that bbhs of tfs from distantly related bacteria are usually not evolutionary orthologs. furthermore, the false orthologs usually respond to different signals and regulate distinct pathways, while the few bbhs that are evolutionary orthologs d ... | 2007 | 17845071 |
| the narqp genes for a two-component regulatory system from the deep-sea bacterium shewanella violacea dss12. | shewanella violacea dss12 is facultative piezophile isolated from the deep-sea. the expression of cyddc genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. in this study, we analyzed the nucleotide sequence upstream of cyddc in detail and found that there are putative binding sites for the narl protein which is part of a two-component regulatory system also containing the sensor protein narx. furthermore, we identified the narqp genes (homologu ... | 2008 | 17852338 |
| evolutionary analysis of enzymes using chisel. | motivation: availability of large volumes of genomic and enzymatic data for taxonomically and phenotypically diverse organisms allows for exploration of the adaptive mechanisms that led to diversification of enzymatic functions. we present chisel, a computational framework and a pipeline for an automated, high-resolution analysis of evolutionary variations of enzymes. chisel allows automatic as well as interactive identification, and characterization of enzymatic sequences. such knowledge can be ... | 2007 | 17855417 |
| reduction of ferric green rust by shewanella putrefaciens. | to reduce carbonated ferric green rust (gr*) using an iron respiring bacterium and obtain its reduced homologue, the mixed fe(ii)-fe(iii) carbonated green rust (gr). | 2007 | 17868312 |
| hydrogenase- and outer membrane c-type cytochrome-facilitated reduction of technetium(vii) by shewanella oneidensis mr-1. | pertechnetate, (99)tc(vii)o(4)(-), is a highly mobile radionuclide contaminant at us department of energy sites that can be enzymatically reduced by a range of anaerobic and facultatively anaerobic microorganisms, including shewanella oneidensis mr-1, to poorly soluble tc(iv)o(2(s)). in other microorganisms, tc(vii)o(4)(-) reduction is generally considered to be catalysed by hydrogenase. here, we provide evidence that although the nife hydrogenase of mr-1 was involved in the h(2)-driven reductio ... | 2008 | 17888007 |
| development of biatech-54 standard mixtures for assessment of protein identification and relative expression. | mixtures of known proteins have been very useful in the assessment and validation of methods for high-throughput (htp) ms (ms/ms) proteomics experiments. however, these test mixtures have generally consisted of few proteins at near equal concentration or of a single protein at varied concentrations. such mixtures are too simple to effectively assess the validity of error rates for protein identification and differential expression in htp ms/ms studies. this work aimed at overcoming these limitat ... | 2007 | 17890649 |
| absolute configuration of 8-amino-3,8-dideoxyoct-2-ulosonic acid, the chemical hallmark of lipopolysaccharides of the genus shewanella. | the novel monosaccharide 8-amino-3,8-dideoxyoct-2-ulosonic acid (kdo8n) was isolated by methanolysis from the lipooligosaccharide of the marine gram-negative bacterium shewanella pacifica. after hplc purification, the absolute configuration was determined by the mosher ester method and proven to be 4 r,5 r,6 r,7 r. this established the d- manno- configuration of the monosaccharide. | 2007 | 17914882 |
| characterisitics and gene cloning of phospholipase d of the psychrophile, shewanella sp. | phospholipase d, with a molecular mass of 64 kda, was purified from the psychrophile, shewanella sp. the enzyme showed maximal activity at ph 7.8 and 40 degrees c in the presence of the ca2+-ion, and its activity at 10 degrees c was 6.5% of maximum. the enzyme exhibited high activity to the non-micelle form of phosphatidylcholine in an aqueous solution containing water miscible alcohols such as methanol, ethanol, iso-propanol, and n-propanol. nucleotide sequencing of the enzyme gene yielded a de ... | 2007 | 17928713 |
| oxygen exposure promotes fuel diversity for shewanella oneidensis microbial fuel cells. | miniature microbial fuel cells (mini-mfcs) were used to monitor the current generated by shewanella oneidensis dsp10 under both anaerobic and aerobic conditions when exposed to glucose as a potential electron donor. in addition to glucose, other carbon fuels including fructose, sucrose, acetate, and ascorbic acid were also tested. when the anolyte containing s. oneidensis was grown in the presence of oxygen, power densities of 270+/-10, 350+/-20, and 120+/-10 w/m(3) were recorded from the mini-m ... | 2008 | 17931851 |
| many microbe microarrays database: uniformly normalized affymetrix compendia with structured experimental metadata. | many microbe microarrays database (m3d) is designed to facilitate the analysis and visualization of expression data in compendia compiled from multiple laboratories. m3d contains over a thousand affymetrix microarrays for escherichia coli, saccharomyces cerevisiae and shewanella oneidensis. the expression data is uniformly normalized to make the data generated by different laboratories and researchers more comparable. to facilitate computational analyses, m3d provides raw data (cel file) and nor ... | 2008 | 17932051 |
| speciation of vanadium in oilsand coke and bacterial culture by high performance liquid chromatography inductively coupled plasma mass spectrometry. | a simple and sensitive method for the speciation of vanadium(iii), (iv), and (v) was developed by using high performance liquid chromatography and inductively coupled plasma mass spectrometry (hplc-icpms). the edta-complexed vanadium species were separated on a strong anion exchange column with an eluent containing 2 mm edta, 3% acetonitrile, and 80 mm ammonium bicarbonate at ph 6. each analysis was complete in 5 min. the detection limits were 0.6, 0.7 and 1.0 microg l(-1) for v(iii), v(iv), and ... | 2007 | 17936102 |
| mechanisms of electron transfer in two decaheme cytochromes from a metal-reducing bacterium. | in this report, we analyze and interpret single-molecule current-voltage (i-v) tunneling spectra collected for two decaheme c-type cytochromes using a scanning tunneling microscope. the cytochromes (omca and mtrc) are outer-membrane proteins from the metal-reducing bacterium shewanella oneidensis and function as metal-reducing enzymes. although the two cytochromes are similar in heme count, charge-carrying amino acid content, and molecular mass, their i-v spectra are significantly different. the ... | 2007 | 17939701 |
| [bacterial community structure in intestine of the white shrimp, litopenaeus vannamei]. | the composition of bacterial community in the intestine of the white shrimp, litopenaeus vannamei under laboratory culture condition was determined using the 16s rdna clone library. 16s rrna gene was amplified and a library was constructed by using the genomic dna extracted from the bacteria in the shrimp intestine as template. 12 different rflp patterns of the clones were obtained by restriction fragment length polymorphism analysis using afa i and msp i. compared with the published sequences i ... | 2007 | 17944366 |
| mrna-targeted fluorescent in situ hybridization (fish) of gram-negative bacteria without template amplification or tyramide signal amplification. | technologies are needed to study gene expression at the level of individual cells within a population or microbial community. fluorescent in situ hybridization (fish) supplies high-resolution spatial information and has been widely applied to study microbial communities at the rrna level. while mrna-targeted fish has been popular for studying gene expression in eukaryotic cells, very little success has been achieved with prokaryotes. at present, detection of specific mrnas in individual prokaryo ... | 2007 | 17949838 |
| characterization of the arsenate respiratory reductase from shewanella sp. strain ana-3. | microbial arsenate respiration contributes to the mobilization of arsenic from the solid to the soluble phase in various locales worldwide. to begin to predict the extent to which as(v) respiration impacts arsenic geochemical cycling, we characterized the expression and activity of the shewanella sp. strain ana-3 arsenate respiratory reductase (arr), the key enzyme involved in this metabolism. arr is expressed at the beginning of the exponential phase and persists throughout the stationary phase ... | 2008 | 17951391 |
| cerebellar abscess and meningitis, caused by shewanella putrefaciens and klebsiella pneumoniae, associated with chronic otitis media. | shewanella putrefaciens is a facultatively anaerobic, non-motile, gram-negative, non-fermentative bacterium. it is found in various environments and has been isolated worldwide. s. putrefaciens is a rare cause of brain abscesses and meningitis. this is a case report of a cerebellar abscess and meningitis caused by shewanella putrefaciens and klebsiella pneumoniae in a river trap fisherman. | 2007 | 17965359 |
| metabolic uncoupling of shewanella oneidensis mr-1, under the influence of excess substrate and 3, 3', 4', 5-tetrachlorosalicylanilide (tcs). | the dissociation between catabolism and anabolism is generally termed as metabolic uncoupling. experimentally, metabolic uncoupling is characterized by a reduction in the observed biomass yield. this condition can be brought about by: (a) excess-substrate (as measured by s(0)/x(0)), and (b) addition of chemical uncouplers such as 3, 3', 4', 5-tetrachlorosalicylanilide (tcs). an empirical model is proposed to quantify the uncoupling effects of both excess-substrate and uncoupler addition on the m ... | 2008 | 17972334 |
| shewanella putrefaciens keratitis in the lamellar bed 6 years after lasik. | to present a case of infectious keratitis occurring 6 years after lasik due to the rare human pathogen shewanella putrefaciens. | 2007 | 17985805 |
| shewanella oneidensis mr-1 h-nox regulation of a histidine kinase by nitric oxide. | nitric oxide (no) signaling in animals controls processes such as smooth muscle relaxation and neurotransmission by activation of soluble guanylate cyclase (sgc). prokaryotic homologues of the sgc heme domain, called h-nox domains, have been identified and are generally found in a predicted operon in conjunction with a histidine kinase. here, we show that an h-nox protein (so2144) from shewanella oneidensis directly interacts with the sensor histidine kinase (so2145), binds no in a 5-coordinate ... | 2007 | 17988156 |
| shelf-life of chilled fresh mediterranean swordfish (xiphias gladius) stored under various packaging conditions: microbiological, biochemical and sensory attributes. | the present work evaluated the effect of air, vacuum and modified atmosphere packaging (map) on the shelf-life of chilled mediterranean swordfish (xiphias gladius). fresh swordfish slices were stored in air, under vacuum and map (40%/30%/30%, co(2)/n(2)/o(2)) under refrigeration (4 degrees c) for a period of 16 days. of the three treatments used (vacuum, map and air), both map and vacuum packaging (vp) were the most effective for inhibiting growth of aerobic microflora in swordfish samples until ... | 2008 | 17993387 |
| growth with high planktonic biomass in shewanella oneidensis fuel cells. | shewanella oneidensis mr-1 grew for over 50 days in microbial fuel cells, incompletely oxidizing lactate to acetate with high recovery of the electrons derived from this reaction as electricity. electricity was produced with lactate or hydrogen and current was comparable to that of electricigens which completely oxidize organic substrates. however, unlike fuel cells with previously described electricigens, in which cells are primarily attached to the anode, at least as many of the s. oneidensis ... | 2008 | 17995953 |
| increased catalytic efficiency following gene fusion of bifunctional methionine sulfoxide reductase enzymes from shewanella oneidensis. | methionine sulfoxide reductase enzymes msra and msrb have complementary stereospecificities that reduce the s and r stereoisomers of methionine sulfoxide (metso), respectively, and together function as critical antioxidant enzymes. in some pathogenic and metal-reducing bacteria, these genes are fused to form a bifunctional methionine sulfoxide reductase (i.e., msrba) enzyme. to investigate how gene fusion affects the substrate specificity and catalytic activities of msr, we have cloned and expre ... | 2007 | 17997579 |
| spatially resolved force spectroscopy of bacterial surfaces using force-volume imaging. | force spectroscopy using the atomic force microscope (afm) is a powerful technique for measuring physical properties and interaction forces at microbial cell surfaces. typically for such a study, the point at which a force measurement will be made is located by first imaging the cell using afm in contact mode. in this study, we image the bacterial cell shewanella putrefaciens for subsequent force measurements using afm in force-volume mode and compare this to contact-mode images. it is known tha ... | 2008 | 18023156 |
| marine bacteria comprise a possible indicator of drowning in seawater. | to investigate the effectiveness of marine bacteria as a new marker of drowning in seawater, we determined the optimal conditions of media required to selectively detect marine bacteria and applied the technique to drowned cadavers. we incubated model blood samples (n=20 per group) mixed with seawater, river, tap or muddy water on agar plates (todd hewitt, th; marine 2216, m2216) and determined the nacl concentration required to selectively detect marine bacteria. we also used tcbs agar plates w ... | 2008 | 18023311 |