| monoclonal antibody therapy for junin virus infection. | countermeasures against potential biothreat agents remain important to us homeland security, and many of these pharmaceuticals could have dual use in the improvement of global public health. junin virus, the causative agent of argentine hemorrhagic fever (ahf), is an arenavirus identified as a category a high-priority agent. there are no food and drug administration (fda) approved drugs available for preventing or treating ahf, and the current treatment option is limited to administration of imm ... | 2016 | 27044104 |
| highly sensitive assay for measurement of arenavirus-cell attachment. | arenaviruses are a family of enveloped rna viruses that cause severe human disease. the first step in the arenavirus life cycle is attachment of viral particles to host cells. while virus-cell attachment can be measured through the use of virions labeled with biotin, radioactive isotopes, or fluorescent dyes, these approaches typically require high multiplicities of infection (moi) to enable detection of bound virus. we describe a quantitative (q)rt-pcr-based assay that measures junin virus stra ... | 2016 | 26966937 |
| [experience of study and possible ways of elimination of false positive and false negative results during execution of polymerase chain reaction on an example of junin virus rna detection]. | experience of study and possible ways of elimination of false positive and false negative results during execution of polymerase chain reaction on an example of junin virus rna detection. materialss and methods: junin virus--causative agent of argentine hemorrhagic fever (ahf) strain xjpr37/5787 was obtained from the state collection of pathogenicity group i causative agents of the 48th central research institute. reagent kit for detection of junin virus rna by rt-pcr was developed in the instit ... | 2016 | 26950993 |
| [experience of study and possible ways of elimination of false positive and false negative results during execution of polymerase chain reaction on an example of junin virus rna detection]. | experience of study and possible ways of elimination of false positive and false negative results during execution of polymerase chain reaction on an example of junin virus rna detection. materialss and methods: junin virus--causative agent of argentine hemorrhagic fever (ahf) strain xjpr37/5787 was obtained from the state collection of pathogenicity group i causative agents of the 48th central research institute. reagent kit for detection of junin virus rna by rt-pcr was developed in the instit ... | 2016 | 26950993 |
| the new world arenavirus tacaribe virus induces caspase-dependent apoptosis in infected cells. | the arenaviridae is a diverse and growing family of viruses that already includes more than 25 distinct species. while some of these viruses have a significant impact on public health, others appear to be non-pathogenic. at present little is known about the host cell responses to infection with different arenaviruses, particularly those found in the new world; however, apoptosis is known to play an important role in controlling infection of many viruses. here we show that infection with tacaribe ... | 2016 | 26769540 |
| low-dose ribavirin potentiates the antiviral activity of favipiravir against hemorrhagic fever viruses. | favipiravir is approved in japan to treat novel or re-emerging influenza viruses, and is active against a broad spectrum of rna viruses, including ebola. ribavirin is the only other licensed drug with activity against multiple rna viruses. recent studies show that ribavirin and favipiravir act synergistically to inhibit bunyavirus infections in cultured cells and laboratory mice, likely due to their different mechanisms of action. convalescent immune globulin is the only approved treatment for a ... | 2016 | 26711718 |
| low-dose ribavirin potentiates the antiviral activity of favipiravir against hemorrhagic fever viruses. | favipiravir is approved in japan to treat novel or re-emerging influenza viruses, and is active against a broad spectrum of rna viruses, including ebola. ribavirin is the only other licensed drug with activity against multiple rna viruses. recent studies show that ribavirin and favipiravir act synergistically to inhibit bunyavirus infections in cultured cells and laboratory mice, likely due to their different mechanisms of action. convalescent immune globulin is the only approved treatment for a ... | 2016 | 26711718 |
| molecular basis for antibody-mediated neutralization of new world hemorrhagic fever mammarenaviruses. | in the western hemisphere, at least five mammarenaviruses cause human viral hemorrhagic fevers with high case fatality rates. junín virus (junv) is the only hemorrhagic fever virus for which transfusion of survivor immune plasma that contains neutralizing antibodies ("passive immunity") is an established treatment. here, we report the structure of the junv surface glycoprotein receptor-binding subunit (gp1) bound to a neutralizing monoclonal antibody. the antibody engages the gp1 site that binds ... | 2015 | 26651946 |
| [new transmission scenarios of the argentine hemorrhagic fever since the introduction of the live attenuated junin virus vaccine (candid #1): an experience in migrant workers]. | the argentine hemorrhagic fever (ahf) is a severe acute viral disease caused by the junin virus of the arenaviridae family. the ahf endemic area coincides geographically with the largest grain export agro-industrial complex of the country [argentina]. since the implementation of vaccination with the candid #1 vaccine, a significant reduction in incidence was achieved and risk patterns were modified. a previous study allowed characterizing these changes and identifying three transmission scenario ... | 2015 | 26102120 |
| novel arenavirus entry inhibitors discovered by using a minigenome rescue system for high-throughput drug screening. | certain members of the arenaviridae family are category a agents capable of causing severe hemorrhagic fevers in humans. specific antiviral treatments do not exist, and the only commonly used drug, ribavirin, has limited efficacy and can cause severe side effects. the discovery and development of new antivirals are inhibited by the biohazardous nature of the viruses, making them a relatively poorly understood group of human pathogens. we therefore adapted a reverse-genetics minigenome (mg) rescu ... | 2015 | 26041296 |
| [sensitivity and specificity of the elisa kit for the detection of antidobies to junin virus]. | the goal of this work was to describe methodological approaches to determination of sensitivity and specificity of the enzyme-linked immunosorbent assay kit (elisa kit) for detection of the specific anti-junin virus (jv) antibody. comparison of elisa to plaque reduction neutralization test (prnt) showed direct relationship between antibody titers in the samples of serum of immunized animals, determined by either prnt or elisa methods. the obtained results provided an opportunity to form the pane ... | 2015 | 26021075 |
| [sensitivity and specificity of the elisa kit for the detection of antidobies to junin virus]. | the goal of this work was to describe methodological approaches to determination of sensitivity and specificity of the enzyme-linked immunosorbent assay kit (elisa kit) for detection of the specific anti-junin virus (jv) antibody. comparison of elisa to plaque reduction neutralization test (prnt) showed direct relationship between antibody titers in the samples of serum of immunized animals, determined by either prnt or elisa methods. the obtained results provided an opportunity to form the pane ... | 2015 | 26021075 |
| highly pathogenic new world and old world human arenaviruses induce distinct interferon responses in human cells. | the arenavirus family includes several important pathogens that cause severe and sometimes fatal diseases in humans. the highly pathogenic old world (ow) arenavirus lassa fever virus (lasv) is the causative agent of lassa fever (lf) disease in humans. lasv infections in severe cases are generally immunosuppressive without stimulating interferon (ifn) induction, a proinflammatory response, or t cell activation. however, the host innate immune responses to highly pathogenic new world (nw) arenavir ... | 2015 | 25926656 |
| human plasmacytoid dendritic cells elicited different responses after infection with pathogenic and nonpathogenic junin virus strains. | the arenavirus junin virus (junv) is the etiologic agent of argentine hemorrhagic fever. we characterized the junv infection of human peripheral blood-derived plasmacytoid dendritic cells (hpdc), demonstrating that hpdc are susceptible to infection with the c#1 strain (attenuated) and even more susceptible to infection with the p (virulent) junv strain. however, hpdc elicited different responses in terms of viability, activation, maturation, and cytokine expression after infection with both junv ... | 2015 | 25926646 |
| the glycoprotein precursor gene of junin virus determines the virulence of the romero strain and the attenuation of the candid #1 strain in a representative animal model of argentine hemorrhagic fever. | the new world arenavirus junin virus (junv) is the causative agent of argentine hemorrhagic fever (ahf), a potentially deadly disease endemic to central regions of argentina. the live-attenuated candid #1 (can) strain of junv is currently used to vaccinate the human population at risk. however, the mechanism of attenuation of this strain is still largely unknown. therefore, the identification and functional characterization of viral genetic determinants dictating junv virulence or attenuation wo ... | 2015 | 25810546 |
| the glycoprotein precursor gene of junin virus determines the virulence of the romero strain and the attenuation of the candid #1 strain in a representative animal model of argentine hemorrhagic fever. | the new world arenavirus junin virus (junv) is the causative agent of argentine hemorrhagic fever (ahf), a potentially deadly disease endemic to central regions of argentina. the live-attenuated candid #1 (can) strain of junv is currently used to vaccinate the human population at risk. however, the mechanism of attenuation of this strain is still largely unknown. therefore, the identification and functional characterization of viral genetic determinants dictating junv virulence or attenuation wo ... | 2015 | 25810546 |
| sorting of small infectious virus particles by flow virometry reveals distinct infectivity profiles. | the nature and concentration of lipids and proteins at the surface of viruses are essential parameters for determining particle infectiveness. historically, averaged bulk analysis of viral particles has been the primary method to quantitatively investigate these parameters, though this neglects heterogeneity within populations. here we analyse the properties of junin virus particles using a sensitive flow virometry assay and further sort virions while conserving their infectiveness. this method ... | 2015 | 25641385 |
| research efforts to control highly pathogenic arenaviruses: a summary of the progress and gaps. | significant progress has been made in the past 10 years in unraveling the molecular biology of highly pathogenic arenaviruses that are endemic in several west african countries (lassa fever virus) and in some regions of south america (argentine and bolivian hemorrhagic fever viruses). while this has resulted in proof-of-concept studies of novel vaccine candidates in non-human primates and in the discovery of several novel antiviral small molecule drug candidates, none of them has been tested in ... | 2015 | 25549822 |
| inhibition of the pi3k/akt pathway by ly294002 does not prevent establishment of persistent junín virus infection in vero cells. | in previous work, we demonstrated that the arenavirus junín virus (junv) is able to activate akt by means of the phosphatidylinositol-3-kinase (pi3k) survival pathway during virus entry. this work extends our study, emphasizing the relevance of this pathway in the establishment and maintenance of persistent infection in vitro. during the course of infection, junv-infected vero cells showed a typical cytopathic effect that may be ascribed to apoptotic cell death. treatment of infected cultures wi ... | 2015 | 25488290 |
| [study of sensitivity of laboratory animals to a causative agent of argentine hemorrhagic fever]. | study sensitivity of laboratory animals to a causative agent ofargentine hemorrhagic fever. | 2014 | 25286529 |
| [study of sensitivity of laboratory animals to a causative agent of argentine hemorrhagic fever]. | study sensitivity of laboratory animals to a causative agent ofargentine hemorrhagic fever. | 2014 | 25286529 |
| rig-i enhanced interferon independent apoptosis upon junin virus infection. | junin virus (junv) is the etiological agent of argentine hemorrhagic fever (ahf), a human disease with a high case-fatality rate. it is widely accepted that arenaviral infections, including junv infections, are generally non-cytopathic. in contrast, here we demonstrated apoptosis induction in human lung epithelial carcinoma (a549), human hepatocarcinoma and vero cells upon infection with the attenuated candid#1 strain of, junv as determined by phosphatidylserine (ps) translocation, caspase 3 (ca ... | 2014 | 24918927 |
| toll-like receptor 2-mediated innate immune responses against junín virus in mice lead to antiviral adaptive immune responses during systemic infection and do not affect viral replication in the brain. | successful adaptive immunity to virus infection often depends on the initial innate response. previously, we demonstrated that junín virus, the etiological agent responsible for argentine hemorrhagic fever (ahf), activates an early innate immune response via an interaction between the viral glycoprotein and toll-like receptor 2 (tlr2). here we show that tlr2/6 but not tlr1/2 heterodimers sense junín virus glycoprotein and induce a cytokine response, which in turn upregulates the expression of th ... | 2014 | 24760892 |
| raf/mek/erk pathway activation is required for junín virus replication. | in the present work we investigated the importance of the raf/mek/erk signalling pathway in the multiplication of the arenavirus junín (junv) in monkey and human cell cultures. we established that junv induces a biphasic activation of erk and we proved that a specific inhibitor of the erk pathway, u0126, impairs viral replication. furthermore, u0126 exerted inhibitory action against the arenaviruses tacaribe and pichinde. moreover, treatment with known erk activators such as phorbol 12-myristate ... | 2014 | 24421112 |
| watching every step of the way: junín virus attenuation markers in the vaccine lineage. | the arenaviridae family includes several hemorrhagic fever viruses which are important emerging pathogens. junín virus, a member of this family, is the etiological agent of argentine hemorrhagic fever (ahf). a collaboration between the governments of argentina and the usa rendered the attenuated junín virus vaccine strain candid#1. arenaviruses are enveloped viruses with genomes consisting of two single-stranded rna species (l and s), each carrying two coding regions separated by a stably struct ... | 2013 | 24396274 |
| favipiravir (t-705) inhibits junín virus infection and reduces mortality in a guinea pig model of argentine hemorrhagic fever. | junín virus (junv), the etiologic agent of argentine hemorrhagic fever (ahf), is classified by the niaid and cdc as a category a priority pathogen. presently, antiviral therapy for ahf is limited to immune plasma, which is readily available only in the endemic regions of argentina. t-705 (favipiravir) is a broadly active small molecule rna-dependent rna polymerase inhibitor presently in clinical evaluation for the treatment of influenza. we have previously reported on the in vitro activity of fa ... | 2013 | 24386500 |
| favipiravir (t-705) inhibits junín virus infection and reduces mortality in a guinea pig model of argentine hemorrhagic fever. | junín virus (junv), the etiologic agent of argentine hemorrhagic fever (ahf), is classified by the niaid and cdc as a category a priority pathogen. presently, antiviral therapy for ahf is limited to immune plasma, which is readily available only in the endemic regions of argentina. t-705 (favipiravir) is a broadly active small molecule rna-dependent rna polymerase inhibitor presently in clinical evaluation for the treatment of influenza. we have previously reported on the in vitro activity of fa ... | 2013 | 24386500 |
| targeting of arenavirus rna synthesis by a carboxamide-derivatized aromatic disulfide with virucidal activity. | several arenaviruses can cause severe hemorrhagic fever (hf) in humans, representing a public health threat in endemic areas of africa and south america. the present study characterizes the potent virucidal activity of the carboxamide-derivatized aromatic disulfide nsc4492, an antiretroviral zinc finger-reactive compound, against junín virus (junv), the causative agent of argentine hf. the compound was able to inactivate junv in a time and temperature-dependent manner, producing more than 99 % r ... | 2013 | 24278404 |
| utilization of human dc-sign and l-sign for entry and infection of host cells by the new world arenavirus, junín virus. | the target cell tropism of enveloped viruses is regulated by interactions between viral proteins and cellular receptors determining susceptibility at a host cell, tissue or species level. however, a number of additional cell-surface moieties can also bind viral envelope glycoproteins and could act as capture receptors, serving as attachment factors to concentrate virus particles on the cell surface, or to disseminate the virus infection to target organs or susceptible cells within the host. here ... | 2013 | 24183720 |
| sirna screen for genes that affect junín virus entry uncovers voltage-gated calcium channels as a therapeutic target. | new world hemorrhagic fever arenavirus infection results in 15 to 30% mortality in humans. we performed a high-throughput small interfering rna screen with junín virus glycoprotein-pseudotyped viruses to find potential host therapeutic targets. voltage-gated calcium channel (vgcc) subunits, for which there are food and drug administration (fda)-approved drugs, were identified in the screen. knockdown of vgcc subunits or treatment with channel blockers diminished junín virus-cell fusion and entry ... | 2013 | 24068738 |
| comparative analysis of disease pathogenesis and molecular mechanisms of new world and old world arenavirus infections. | arenaviruses can cause fatal human haemorrhagic fever (hf) diseases for which vaccines and therapies are extremely limited. both the new world (nw) and old world (ow) groups of arenaviruses contain hf-causing pathogens. although these two groups share many similarities, important differences with regard to pathogenicity and molecular mechanisms of virus infection exist. these closely related pathogens share many characteristics, including genome structure, viral assembly, natural host selection ... | 2014 | 24068704 |
| [phenotypic markers of attenuation in junin virus strains recently isolated from individuals vaccinated with junin candid#1 strain]. | argentine hemorrhagic fever is a severe acute disease caused by junin virus. for prevention of this disease an effective vaccine called candid#1 has been developed, composed of a live attenuated junin virus strain. during a clinical trial conducted at instituto nacional de enfermedades virales humanas (inevh) in 2005, junin virus was isolated from two vaccinated volunteers by co-culture of peripheral mononuclear blood cells. the aim of this study was to compare the strains isolated from these hu ... | 2013 | 23924527 |
| [phenotypic markers of attenuation in junin virus strains recently isolated from individuals vaccinated with junin candid#1 strain]. | argentine hemorrhagic fever is a severe acute disease caused by junin virus. for prevention of this disease an effective vaccine called candid#1 has been developed, composed of a live attenuated junin virus strain. during a clinical trial conducted at instituto nacional de enfermedades virales humanas (inevh) in 2005, junin virus was isolated from two vaccinated volunteers by co-culture of peripheral mononuclear blood cells. the aim of this study was to compare the strains isolated from these hu ... | 2013 | 23924527 |
| crystal structure of junin virus nucleoprotein. | junin virus (junv) has been identified as the aetiological agent of argentine haemorrhagic fever (ahf), which is a serious public health problem with approximately 5 million people at risk. it is treated as a potential bioterrorism agent because of its rapid transmission by aerosols. junv is a negative-sense ssrna virus that belongs to the genus arenavirus within the family arenaviridae, and its genomic rna contains two segments encoding four proteins. among these, the nucleoprotein (np) has ess ... | 2013 | 23884367 |
| [optimization of conditions of quantitative evaluation of argentine hemorrhagic fever causative agent]. | optimization of conditions of quantitative evaluation of argentine hemorrhagic fever causative agent. | 2013 | 23805673 |
| participation of eif4f complex in junin virus infection: blockage of eif4e does not impair virus replication. | translation efficiency of viral mrnas is a key factor defining both cytopathogenicity and virulence of viruses, which are entirely dependent on the cellular translation machinery to synthesize their proteins. this dependence has led them to develop different translational reprogramming strategies to ensure viral mrnas can effectively compete with cellular mrnas. junin virus (junv) is a member of the family arenaviridae, whose mrnas are capped but not polyadenylated. in this work we evaluated the ... | 2013 | 23601822 |
| membrane localization of junín virus glycoproteins requires cholesterol and cholesterol rich membranes. | arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, the nature of membrane assembly sites remains poorly understood. in this study we examined the effect of different cholesterol-lowering agents on junín virus (junv) multiplication. we found that cholesterol cell depletion reduced junv glycoproteins (gps) membrane expression and virus budding. analysis of membrane protein insolubility in triton x-100 suggested that junv gps associate with cholesterol enriched membra ... | 2013 | 23261443 |
| junín virus pathogenesis and virus replication. | junín virus, the etiological agent of argentine hemorrhagic fever, causes significant morbidity and mortality. the virus is spread through the aerosolization of host rodent excreta and endemic to the humid pampas of argentina. recently, significant progress has been achieved with the development of new technologies (e.g. reverse genetics) that have expanded knowledge about the pathogenesis and viral replication of junín virus. we will review the pathogenesis of junín virus in various animal mode ... | 2012 | 23202466 |
| host cell factors as antiviral targets in arenavirus infection. | among the members of the arenaviridae family, lassa virus and junin virus generate periodic annual outbreaks of severe human hemorrhagic fever (hf) in endemic areas of west africa and argentina, respectively. given the human health threat that arenaviruses represent and the lack of a specific and safe chemotherapy, the search for effective antiviral compounds is a continuous demanding effort. since diverse host cell pathways and enzymes are used by rna viruses to fulfill their replicative cycle, ... | 2012 | 23170173 |
| antigen vehiculization particles based on the z protein of junin virus. | arenavirus matrix protein z plays an important role in virus budding and is able to generate enveloped virus-like-particles (vlps) in absence of any other viral proteins. in these vlps, z protein is associated to the plasma membrane inner surface by its myristoyl residue. budding induction and vesicle formation properties can be exploited to generate enveloped vlps platform. these structures can be designed to carry specific antigen in the inner side or on the surface of vlps.vaccines based on v ... | 2012 | 23121996 |
| cleavage of the junin virus nucleoprotein serves a decoy function to inhibit the induction of apoptosis during infection. | the regulation of apoptosis during infection is an important factor for host survival and, in some cases, also for the virus life cycle. at the same time, mechanisms to prevent the induction of apoptosis have been observed in numerous viral pathogens, but until now the role of apoptosis during arenavirus infection has not been investigated. junin virus (junv) belongs to the new world arenavirus serogroup of the arenaviridae and is the causative agent of argentine hemorrhagic fever. we have demon ... | 2013 | 23077297 |
| arenavirus infection induces discrete cytosolic structures for rna replication. | arenaviruses are responsible for acute hemorrhagic fevers with high mortality and pose significant threats to public health and biodefense. these enveloped negative-sense rna viruses replicate in the cell cytoplasm and express four proteins. to better understand how these proteins insinuate themselves into cellular processes to orchestrate productive viral replication, we have identified and characterized novel cytosolic structures involved in arenavirus replication and transcription. in cells i ... | 2012 | 22875974 |
| [argentine hemorrhagic fever]. | features of the argentine hemorrhagic fever are described in the review. epidemiology, etiology, clinical presentation and pathogenesis of the disease are examined. special consideration is given to the features of the pathological agent of argentine hemorrhagic fever--the junin virus. features of the disease diagnostics are indicated--virological and serological studies, immunochemical and molecular-biological methods of identification of the pathological agent and antibodies against it. approa ... | 2012 | 22693817 |
| s-layer proteins of lactobacillus acidophilus inhibits junv infection. | it has been previously described that s-layer binds to the c-type lectin dc-specific icam-3-grabbing nonintegrin (dc-sign, cd209). it was also shown that dc-sign is a cell-surface adhesion factor that enhances viral entry of several virus families. among those, junin virus (junv) entry is enhanced in cells expressing dc-sign and for that reason surface-layer protein (s-layer) of lactobacillus acidophilus atcc 4365 was evaluated as a possible junv inhibitor. experiments using 3t3 cells stably exp ... | 2012 | 22595457 |
| the pi3k/akt pathway contributes to arenavirus budding. | several arenaviruses, chiefly lassa virus (lasv), cause hemorrhagic fever (hf) disease in humans and pose a significant public health concern in regions where they are endemic. on the other hand, evidence indicates that the globally distributed prototypic arenavirus lymphocytic choriomeningitis virus (lcmv) is a neglected human pathogen. the phosphatidylinositol 3-kinase (pi3k)/akt pathway participates in many cellular processes, including cell survival and differentiation, and also has been sho ... | 2012 | 22345463 |
| differential inhibition of macrophage activation by lymphocytic choriomeningitis virus and pichinde virus is mediated by the z protein n-terminal domain. | several arenavirus pathogens, such as lassa and junin viruses, inhibit macrophage activation, the molecular mechanism of which is unclear. we show that lymphocytic choriomeningitis virus (lcmv) can also inhibit macrophage activation, in contrast to pichinde and tacaribe viruses, which are not known to naturally cause human diseases. using a recombinant pichinde virus system, we show that the lcmv z n-terminal domain (ntd) mediates the inhibition of macrophage activation and immune functions. | 2015 | 26423945 |
| rapid screening for entry inhibitors of highly pathogenic viruses under low-level biocontainment. | emerging viruses including nipah, hendra, lujo, and junin viruses have enormous potential to spread rapidly. nipah virus, after emerging as a zoonosis, has also evolved the capacity for human-to-human transmission. most of the diseases caused by these pathogens are untreatable and require high biocontainment conditions. universal methods for rapidly identifying and screening candidate antivirals are urgently needed. we have developed a modular antiviral platform strategy that relies on simple bi ... | 2012 | 22396728 |
| serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers. | the family arenaviridae, genus arenavirus, consists of two phylogenetically independent groups: old world (ow) and new world (nw) complexes. the lassa and lujo viruses in the ow complex and the guanarito, junin, machupo, sabia, and chapare viruses in the nw complex cause viral hemorrhagic fever (vhf) in humans, leading to serious public health concerns. these viruses are also considered potential bioterrorism agents. therefore, it is of great importance to detect these pathogens rapidly and spec ... | 2012 | 23202455 |
| highly pathogenic new world arenavirus infection activates the pattern recognition receptor pkr without attenuating virus replication in human cells. | the arenavirus family consists of several highly pathogenic viruses including the old world (ow) arenavirus lassa fever virus (lasv) and the new world (nw) junín virus (junv) and machupo virus (macv). host response to infection by these pathogenic arenaviruses is distinct in many aspects. nw junv and macv infections readily induce an ifn response in human cells, while ow lasv infection usually triggers an undetectable or weak ifn response. junv induces ifn response through rig-i, suggesting that ... | 2017 | 28794024 |
| enhanced protection against experimental junin virus infection through the use of a modified favipiravir loading dose strategy. | a collection of old and new world arenaviruses are etiologic agents of viral hemorrhagic fever, a syndrome that features hematologic abnormalities, vascular leak, hypovolemia, and multi-organ failure. treatment is limited to ribavirin for lassa fever and immune plasma for argentine hemorrhagic fever. improved therapeutic options that are safe, more effective and widely available are needed. here, we show that modification of favipiravir treatment to include a high-dose loading period achieves co ... | 2017 | 28780425 |
| enhanced protection against experimental junin virus infection through the use of a modified favipiravir loading dose strategy. | a collection of old and new world arenaviruses are etiologic agents of viral hemorrhagic fever, a syndrome that features hematologic abnormalities, vascular leak, hypovolemia, and multi-organ failure. treatment is limited to ribavirin for lassa fever and immune plasma for argentine hemorrhagic fever. improved therapeutic options that are safe, more effective and widely available are needed. here, we show that modification of favipiravir treatment to include a high-dose loading period achieves co ... | 2017 | 28780425 |
| convergent immunological solutions to argentine hemorrhagic fever virus neutralization. | transmission of hemorrhagic fever new world arenaviruses from their rodent reservoirs to human populations poses substantial public health and economic dangers. these zoonotic events are enabled by the specific interaction between the new world arenaviral attachment glycoprotein, gp1, and cell surface human transferrin receptor (htfr1). here, we present the structural basis for how a mouse-derived neutralizing antibody (nab), od01, disrupts this interaction by targeting the receptor-binding surf ... | 2017 | 28630325 |
| a map of the arenavirus nucleoprotein-host protein interactome reveals that junín virus selectively impairs the antiviral activity of double-stranded rna-activated protein kinase (pkr). | arenaviruses are enveloped negative-strand rna viruses that cause significant human disease. these viruses encode only four proteins to accomplish the viral life cycle, so each arenavirus protein likely plays unappreciated accessory roles during infection. here we used immunoprecipitation and mass spectrometry to identify human proteins that interact with the nucleoproteins (nps) of the old world arenavirus lymphocytic choriomeningitis virus (lcmv) and the new world arenavirus junín virus (junv) ... | 2017 | 28539447 |
| differential immune responses to new world and old world mammalian arenaviruses. | some new world (nw) and old world (ow) mammalian arenaviruses are emerging, zoonotic viruses that can cause lethal hemorrhagic fever (hf) infections in humans. while these are closely related rna viruses, the infected hosts appear to mount different types of immune responses against them. lassa virus (lasv) infection, for example, results in suppressed immune function in progressive disease stage, whereas patients infected with junín virus (junv) develop overt pro-inflammatory cytokine productio ... | 2017 | 28498311 |
| ii. attempts to grow tacaribe and junin viruses in grace's continuous line of moth cells. | | 1971 | 5142325 |
| guinea pig transferrin receptor 1 mediates cellular entry of junín virus and other pathogenic new world arenaviruses. | several clade b new world arenaviruses (nwas) can cause severe and often fatal hemorrhagic fever, for which preventive and therapeutic measures are severely limited. these nwas use human transferrin receptor 1 (htfr1) as a host cell receptor for virus entry. the most prevalent of the pathogenic nwas is junín virus (junv), the etiological agent of argentine hemorrhagic fever. small animal models of junv infection are limited because most laboratory rodent species are refractory to disease. only g ... | 2020 | 31748396 |
| [spatial difference in the incidence of argentine hemorrhagic fever and composition and abundance of rodents in the assemblage]. | the argentine hemorrhagic fever (ahf) is a zoonotic disease endemic in a wide area of the humid pampa of argentina. the etiologic agent is the junin virus that is maintained in the wild by the rodent calomys musculinus and transmitted to humans, mainly, through aerosols generated from secretions and excretions. | 2018 | 30534925 |
| a single mutation (v64g) within the ring domain of z attenuates junin virus. | junin virus (junv) is a new world arenavirus that is the causative agent of argentine hemorrhagic fever (ahf). candid#1 (can) is a live-attenuated vaccine strain of junv that since its introduction has resulted in a marked decrease in ahf incidence within the endemic regions of the pampas in argentina. originally, the viral determinants and mechanisms of can attenuation were not well understood. recent work has identified the glycoprotein as the major attenuating factor for can. the establishmen ... | 2020 | 32976538 |
| description and characterization of a novel live-attenuated tri-segmented machupo virus in guinea pigs. | machupo virus (macv) is a member of the mammarenavirus genus, arenaviridae family and is the etiologic agent of bolivian hemorrhagic fever, which causes small outbreaks or sporadic cases. several other arenaviruses in south america junín virus (junv) in argentina, guanarito in venezuela, sabiá in brazil and chapare in bolivia, also are responsible for human hemorrhagic fevers. among these arenaviruses, junv caused thousands of human cases until 1991, when the live attenuated candid #1 vaccine, w ... | 2018 | 29879985 |
| effective population size differences in calomys musculinus, the host of junín virus: their relationship with the epidemiological history of argentine hemorrhagic fever. | argentine hemorrhagic fever (ahf) is a serious endemic disease in argentina, produced by junín virus, whose host is the sigmodontinae rodent calomys musculinus. within the endemic area, human incidence and proportion of infected rodents remains high for 5-10 years after the first appearance of the disease (epidemic [e] zone) and then gradually declines to sporadic cases (historic [h] zone). we tested the hypothesis that host populations within the e zone are large and well connected by gene flow ... | 2018 | 29893205 |
| single dose rvsvδg-junvgp vaccine protects guinea pigs against lethal junin virus challenge. | junin virus (junv) is a pathogen of biodefense importance due to its potential for aerosol transmission and mortality rates reaching 30%. currently, there are no junv vaccines licensed by the united states food and drug administration (fda) for at-risk individuals. a vaccine based on recombinant vesicular stomatitis virus (rvsv) has been effectively used to prevent ebola virus disease in humans. here, we evaluated the protective efficacy of a rvsv expressing the junv glycoprotein (rvsvδg-junvgp) ... | 2021 | 34373461 |
| second-generation live-attenuated candid#1 vaccine virus resists reversion and protects against lethal junín virus infection in guinea pigs. | live-attenuated virus vaccines are highly effective in preventing viral disease but carry intrinsic risks of residual virulence and reversion to pathogenicity. the classically derived candid#1 virus protects seasonal field workers in argentina against zoonotic infection by junín virus (junv) but is not approved in the united states, in part due to the potential for reversion at the attenuating locus, a phenylalanine-to-isoleucine substitution at position 427 in the gp2 subunit of the gpc envelop ... | 2021 | 33952638 |
| therapy for argentine hemorrhagic fever in nonhuman primates with a humanized monoclonal antibody. | the covid-19 pandemic has reemphasized the need to identify safe and scalable therapeutics to slow or reverse symptoms of disease caused by newly emerging and reemerging viral pathogens. recent clinical successes of monoclonal antibodies (mabs) in therapy for viral infections demonstrate that mabs offer a solution for these emerging biothreats. we have explored this with respect to junin virus (junv), an arenavirus classified as a category a high-priority agent and the causative agent of argenti ... | 2021 | 33836604 |
| development of a reverse genetic system to generate recombinant chimeric tacaribe virus that expresses junín virus glycoproteins. | mammarenaviruses are enveloped and segmented negative-stranded rna viruses that comprise several pathogenic members associated with severe human hemorrhagic fevers. tacaribe virus (tcrv) is the prototype for the new world group of mammarenaviruses and is not only naturally attenuated but also phylogenetically and antigenically related to all south american pathogenic mammarenaviruses, particularly the junín virus (junv), which is the etiological agent of argentinian hemorrhagic fever (ahf). more ... | 2020 | 33203040 |