| prevalence of the puumala orthohantavirus strains in the pre-kama area of the republic of tatarstan, russia. | puumala orthohantavirus (puuv) causes nephropathia epidemica (ne), a mild form of hemorrhagic fever with renal syndrome (hfrs) commonly diagnosed in europe. the majority of hfrs cases in the european part of russia are diagnosed in the volga federal district, which includes the republic of tatarstan (rt). the current study aims to analyze the genetic variability of puuv in pre-kama region of the rt bounded by the volga, kama, and vyatka rivers. in 2017, bank voles were caught in seven isolated f ... | 2020 | 32640614 |
| common vole (microtus arvalis) and bank vole (myodes glareolus) derived permanent cell lines differ in their susceptibility and replication kinetics of animal and zoonotic viruses. | pathogenesis and reservoir host adaptation of animal and zoonotic viruses are poorly understood due to missing adequate cell culture and animal models. the bank vole (myodes glareolus) and common vole (microtus arvalis) serve as hosts for a variety of zoonotic pathogens. for a better understanding of virus association to a putative animal host, we generated two novel cell lines from bank voles of different evolutionary lineages and two common vole cell lines and assayed their susceptibility, rep ... | 2019 | 31513859 |
| isolation and characterization of new puumala orthohantavirus strains from germany. | orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. here, we report the isolation of a puumala orthohantavirus (puuv) strain from bank voles caught in a highly endemic region around the city osnabrück, north-west germany. coding and non-coding sequences of all three segments (s, m, and l) were determined from original lung tissue, after isolation and after additional passaging in veroe6 cells and a bank vole-derived kidney cell line. different single amino ac ... | 2020 | 32328924 |
| orthohantavirus isolated in reservoir host cells displays minimal genetic changes and retains wild-type infection properties. | : orthohantaviruses are globally emerging zoonotic pathogens. while the reservoir host role of several rodent species is well-established, detailed research on the mechanisms of host-othohantavirus interactions has been constrained by the lack of an experimental system that is able to effectively replicate natural infections in controlled settings. here we report the isolation, and genetic and phenotypic characterization of a novel puumala orthohantavirus (puuv) in cells derived from its reservo ... | 2020 | 32316667 |
| wildfire-induced short-term changes in a small mammal community increase prevalence of a zoonotic pathogen? | natural disturbances like droughts and fires are important determinants of wildlife community structure and are suggested to have important implications for prevalence of wildlife-borne pathogens. after a major wildfire affecting >1,600 ha of boreal forest in sweden in 2006, we took the rare opportunity to study the short-term response (2007-2010 and 2015) of small mammal community structure, population dynamics, and prevalence of the puumala orthohantavirus (puuv) hosted by bank voles (myodes g ... | 2019 | 31788190 |
| heterogeneous puumala orthohantavirus situation in endemic regions in germany in summer 2019. | puumala orthohantavirus (puuv) causes most human hantavirus disease cases in europe. puuv disease outbreaks are usually synchronized germany-wide driven by beech mast-induced irruptions of its host (bank vole, myodes glareolus). recent data indicate high vole abundance, high puuv prevalence and high human incidence in summer 2019 for some regions, but elsewhere values were low to moderate. this significant lack of synchrony among regions in germany is in contrast to previous studies. health inst ... | 2020 | 31674714 |
| phylogeography of puumala orthohantavirus in europe. | puumala virus is an rna virus hosted by the bank vole (myodes glareolus) and is today present in most european countries. whilst it is generally accepted that hantaviruses have been tightly co-evolving with their hosts, puumala virus (puuv) evolutionary history is still controversial and so far has not been studied at the whole european level. this study attempts to reconstruct the phylogeographical spread of modern puuv throughout europe during the last postglacial period in the light of an upg ... | 2019 | 31344894 |
| cytokine storm combined with humoral immune response defect in fatal hemorrhagic fever with renal syndrome case, tatarstan, russia. | hemorrhagic fever with renal syndrome (hfrs) is endemic in tatarstan, where thousands of cases are registered annually. puumalaorthohantavirus is commonly detected in human case samples as well as in captured bank voles, the rodent hosts. the pathogenesis of hfrs is still not well described, although the cytokine storm hypothesis is largely accepted. in this study, we present a comprehensive analysis of a fatal hfrs case compared with twenty four non-fatal cases where activation of the humoral a ... | 2019 | 31269734 |
| identifying the patterns and drivers of puumala hantavirus enzootic dynamics using reservoir sampling. | hantaviruses are zoonotic hemorrhagic fever viruses for which prevention of human spillover remains the first priority in disease management. tailored intervention measures require an understanding of the drivers of enzootic dynamics, commonly inferred from distorted human incidence data. here, we use longitudinal sampling of approximately three decades of puumala orthohantavirus (puuv) evolution in isolated reservoir populations to estimate puuv evolutionary rates, and apply these to study the ... | 2019 | 31024739 |
| molecular identification of puumala orthohantavirus in bulgaria. | in bulgaria, only dobrava orthohantavirus has been detected in patients and in rodents. in order to elucidate possible puumala orthohantavirus (puuv) circulation, 131 bank voles (myodes glareolus) were captured. puuv rna was detected in 14 (10.7%). partial l segment sequences were recovered from six m. glareolus. phylogenetic analysis showed that all puuv sequences from this study clustered together among the alpe-adrian lineage. it is the first genetic evidence of the virus circulation in a bal ... | 2019 | 30798033 |
| prevalence of orthohantavirus-reactive antibodies in humans and peri-domestic rodents in northern ethiopia. | in 2012, tigray orthohantavirus was discovered in ethiopia, but its seasonal infection in small mammals, and whether it poses a risk to humans was unknown. the occurrence of small mammals, rodents and shrews, in human inhabitations in northern ethiopia is affected by season and presence of stone bunds. we sampled small mammals in two seasons from low- and high-density stone bund fields adjacent to houses and community-protected semi-natural habitats in atsbi and hagere selam, where tigray orthoh ... | 2021 | 34199600 |
| hantavirus-leptospira coinfections in small mammals from central germany. | european orthohantaviruses (puumala orthohantavirus (puuv); dobrava-belgrade orthohantavirus (dobv), genotype kurkino; tula orthohantavirus (tulv)), and leptospira spp. are small mammal-associated zoonotic pathogens that cause diseases with potentially similar symptoms in humans. we investigated the frequency of leptospira spp. and hantavirus single and double infections in small mammals from 22 sites in thuringia, central germany, during 2017. tulv infections were detected at 18 of 22 sites (me ... | 2021 | 33612134 |
| rodent-borne orthohantaviruses in vietnam, madagascar and japan. | hantaviruses are harbored by multiple small mammal species in asia, europe, africa, and the americas. to ascertain the geographic distribution and virus-host relationships of rodent-borne hantaviruses in japan, vietnam, myanmar, and madagascar, rnalater™-preserved lung tissues of 981 rodents representing 40 species, collected in 2011-2017, were analyzed for hantavirus rna by rt-pcr. our data showed hantaan orthohantavirus da bie shan strain in the chinese white-bellied rat (niviventer confucianu ... | 2021 | 34372549 |
| rodent host population dynamics drive zoonotic lyme borreliosis and orthohantavirus infections in humans in northern europe. | zoonotic diseases, caused by pathogens transmitted between other vertebrate animals and humans, pose a major risk to human health. rodents are important reservoir hosts for many zoonotic pathogens, and rodent population dynamics affect the infection dynamics of rodent-borne diseases, such as diseases caused by hantaviruses. however, the role of rodent population dynamics in determining the infection dynamics of rodent-associated tick-borne diseases, such as lyme borreliosis (lb), caused by borre ... | 2021 | 34373474 |
| impact of landscape on host-parasite genetic diversity and distribution using the puumala orthohantavirus-bank vole system. | in nature, host specificity has a strong impact on the parasite's distribution, prevalence, and genetic diversity. the host's population dynamics is expected to shape the distribution of host-specific parasites. in turn, the parasite's genetic structure is predicted to mirror that of the host. here, we study the tandem puumala orthohantavirus (puuv)-bank vole system. the genetic diversity of 310 bank voles and 33 puuv isolates from 10 characterized localities of northeast france was assessed. ou ... | 2021 | 34361952 |
| climate change accelerates winter transmission of a zoonotic pathogen. | many zoonotic diseases are weather sensitive, raising concern how their distribution and outbreaks will be affected by climate change. at northern high latitudes, the effect of global warming on especially winter conditions is strong. by using long term monitoring data (1980-1986 and 2003-2013) from northern europe on temperature, precipitation, an endemic zoonotic pathogen (puumala orthohantavirus, puuv) and its reservoir host (the bank vole, myodes glareolus), we show that early winters have b ... | 2021 | 34228253 |
| genetic diversity of puumala orthohantavirus in rodents and human patients in austria, 2012-2019. | puumala orthohantavirus (puuv) has a wide distribution throughout europe. distinctive temporal patterns of spillover into the human population are related to population dynamics of the reservoir host, the bank vole (clethrionomys glareolus). as the rodent host is tied to specific habitats with small individual ranges, puuv genetic diversity is also highly correlated with geographic distance. using sequenced portions of viral s and m segments, we determined whether geographic clusters were suppor ... | 2021 | 33918083 |
| isolation and genetic characterization of puumala orthohantavirus strains from france. | puumala orthohantavirus (puuv) causes a mild form of haemorrhagic fever with renal syndrome (hfrs) called nephropathia epidemica (ne), regularly diagnosed in europe. france represents the western frontier of the expansion of ne in europe with two distinct areas: an endemic area (north-eastern france) where puuv circulates in rodent populations, with the detection of many human ne cases, and a non-endemic area (south-western france) where the virus is not detected, with only a few human cases bei ... | 2021 | 33809526 |