| butyric acid released during milk lipolysis triggers biofilm formation of bacillus species. | bacillus species form biofilms within milking pipelines and on surfaces of equipment in the dairy industry which represent a continuous hygiene problem and can lead to serious economic losses due to food spoilage and equipment impairment. although much is known about the mechanism by which the model organism bacillus subtilis forms biofilms in laboratory mediums in vitro, little is known of how these biofilms are formed in natural environments such as milk. besides, little is known of the signal ... | 2014 | 24801271 |
| the combined effect of pasteurization intensity, water activity, ph and incubation temperature on the survival and outgrowth of spores of bacillus cereus and bacillus pumilus in artificial media and food products. | the objective of the study was to evaluate the combined effects of pasteurization intensity (no heat treatment and 10 min at 70, 80 and 90 °c), water activity (aw) (0.960-0.990), ph (5.5-7.0) and storage temperature (7 and 10 °c) on the survival and outgrowth of psychrotolerant spores of bacillus cereus ff119b and bacillus pumilus ff128a. the experiments were performed in both artificial media and a validation was performed on real food products (cream, béchamel sauce and mixed vegetable soup). ... | 2014 | 24801270 |
| large-scale purification, characterization, and spore outgrowth inhibitory effect of thurincin h, a bacteriocin produced by bacillus thuringiensis sf361. | large-scale purification of the highly hydrophobic bacteriocin thurincin h was accomplished via a novel and simple two-step method: ammonia sulfate precipitation and c18 solid-phase extraction. the inhibition spectrum and stability of thurincin h as well as its antagonistic activity against bacillus cereus f4552 spores were further characterized. in the purification method, secreted proteins contained in the supernatant of a 40 h incubated culture of b. thuringiensis sf361 were precipitated by 6 ... | 2014 | 24798125 |
| a multiplex pcr assay for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in korean ready-to-eat food. | a multiplex polymerase chain reaction (pcr) assay was developed for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in various korean ready-to-eat foods. the six specific primer pairs for multiplex pcr were selected based on the o157 antigen (rfbe) gene of e. coli o157:h7, the dna gyrase subunit b (gyrb) gene of b. cereus, the toxin regulatory protein (toxr) gene of v. parahaemolyticu ... | 2014 | 24796416 |
| prevalence, genetic diversity, and host range of tectiviruses among members of the bacillus cereus group. | gil01, bam35, gil16, ap50, and wip1 are tectiviruses preying on the bacillus cereus group. despite the significant contributions of phages in different biological processes, little is known about the dealings taking place between tectiviruses and their gram-positive bacterial hosts. therefore, this work focuses on characterizing the interactions between tectiviruses and the b. cereus group by assessing their occurrence and genetic diversity and evaluating their host range. to study the occurrenc ... | 2014 | 24795369 |
| in vitro evaluation of antimicrobial features of vasopressors. | drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. however studies focusing on antibacterial effects of vasopressor drugs are very rare. this study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors. | 2014 | 24794449 |
| role of structural changes induced in biological membranes by hydrolysable tannins from sumac leaves (rhus typhina l.) in their antihemolytic and antibacterial effects. | in this study, we found that the sumac tannins (rhus typhina l.) exert to a various extent antihemolytic effects and antibacterial activity against bacillus cereus and pseudomonas aeruginosa depending on structural specificity of bacteria and different mechanisms of their toxic action. the sumac tannins exert the most expressed activity against b. cereus. the antihemolytic effect of the sumac tannins seems to be connected to a greater extent with their modifying action on the erythrocyte membran ... | 2014 | 24793101 |
| synergistic effects of naocl and ultrasound combination on the reduction of escherichia coli and bacillus cereus in raw laver. | the current study investigated the synergistic effects of naocl (50-200 ppm)/ultrasound (37 khz, 380 w for 5-100 min) combination on the reduction of escherichia coli and bacillus cereus in raw laver. the synergistic reductions were not dependent on concentrations of naocl and times of ultrasound. synergistic reduction ranged from 0.1 to 0.5 log10 colony-forming units (cfu)/g and 0.1-1.1 log10 cfu/g, respectively, for e. coli and b. cereus, with the largest synergistic reduction in the combinati ... | 2014 | 24785713 |
| computational studies of the first order kinetic reactions for mononuclear copper(ii) complexes having a hard-soft ns donor ligand. | the chelation behaviour of 4-((e)-2-(1-(thiophen-2-yl)ethylidene)hydrazinyl)-1-(4-methoxyphenyl)-1h-pyrrole-3-carbonitrile (hl) towards cu(ii) ions has been investigated. these cu(ii) complexes are characterized by elemental analyses, molar-solid conductance, esr, ftir and electronic spectral studies. also, the kinetic and thermodynamic parameters (ea, a, δh, δs, δg) for all thermal decomposition steps have been evaluated using coats-redfern and horowitz-metzger methods. furthermore, antimicrobi ... | 2014 | 24785091 |
| multitrait plant growth promoting (pgp) rhizobacterial isolates from brassica juncea rhizosphere : keratin degradation and growth promotion. | plant growth promoting (pgp) rhizobacteria, a beneficial microbe colonizing plant roots, enhanced crop productivity and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. the keratinous waste which comprises feathers, hairs, nails, skin and wool creates problem of solid waste management due to presence of highly recalcitrant keratin. the multi traits rhizobacteria effective to remove both keratine from the environment by producing keratinase enzyme and to erad ... | 2014 | 24778758 |
| a rifamycin inactivating phosphotransferase family shared by environmental and pathogenic bacteria. | many environmental bacteria are multidrug-resistant and represent a reservoir of ancient antibiotic resistance determinants, which have been linked to genes found in pathogens. exploring the environmental antibiotic resistome, therefore, reveals the diversity and evolution of antibiotic resistance and also provides insight into the vulnerability of clinically used antibiotics. in this study, we describe the identification of a highly conserved regulatory motif, the rifampin (rif) -associated ele ... | 2014 | 24778229 |
| characterization of the extracellular biodemulsifiers secreted by bacillus cereus lh-6 and the enhancement of demulsifying efficiency by optimizing the cultivation conditions. | a highly efficient demulsifying strain, lh-6, was isolated from petroleum-contaminated soil and identified as bacillus cereus by 16s rdna gene analysis. it achieved 95.61 and 95.40 % demulsifying ratios within 12 h for water-in-oil (w/o) and oil-in-water (o/w) model emulsions, respectively. fourier transform infrared spectroscopy (ft-ir) and thin-layer chromatography (tlc) detections indicated that the lh-6's extracellular biodemulsifiers were different types of lipopeptides for the w/o and o/w ... | 2014 | 24777330 |
| the ability of an antimicrobial agent to penetrate a biofilm is not correlated with its killing or removal efficiency. | the penetration ability of 12 antimicrobial agents, including antibiotics and biocides, was determined against biofilms of b. cereus and p. fluorescens using a colony biofilm assay. the surfactants benzalkonium chloride (bac) and cetyltrimethyl ammonium bromide (ctab), and the antibiotics ciprofloxacin and streptomycin were of interest due to their distinct activities. erythromycin and ctab were retarded by the presence of biofilms, whereas ciprofloxacin and bac were not. the removal and killing ... | 2014 | 24773258 |
| phytochemistry and preliminary assessment of the antibacterial activity of chloroform extract of amburana cearensis (allemão) a.c. sm. against klebsiella pneumoniae carbapenemase-producing strains. | the chloroform extract of the stem bark of amburana cearensis was chemically characterized and tested for antibacterial activity.the extract was analyzed by gas chromatography and mass spectrometry. the main compounds identified were 4-methoxy-3-methylphenol (76.7%), triciclene (3.9%), α -pinene (1.0%), β -pinene (2.2%), and 4-hydroxybenzoic acid (3.1%). preliminary antibacterial tests were carried out against species of distinct morphophysiological characteristics: escherichia coli, salmonella ... | 2014 | 24772183 |
| purification and identification of metabolites produced by bacillus cereus and b. subtilis active against meloidogyne exigua, and their in silico interaction with a putative phosphoribosyltransferase from m. incognita. | to contribute to the development of products to control meloidogyne exigua, the bacteria bacillus cereus and b. subtilis were cultivated in liquid medium to produce metabolites active against this plant-parasitic nematode. fractionation of the crude dichloromethane extracts obtained from the cultures afforded uracil, 9h-purine and dihydrouracil. all compounds were active against m. exigua, the latter being the most efficient. this substance presented a lc50 of 204 µg/ml against the nematode, whi ... | 2014 | 24770454 |
| water-based oligochitosan and nanowhisker chitosan as potential food preservatives for shelf-life extension of minced pork. | water-based chitosans in the forms of oligochitosan (oligocs) and nanowhisker chitosan (cswk) are proposed as a novel food preservative based on a minced pork model study. the high surface area with a positive charge over the neutral ph range (ph 5-8) of oligocs and cswk lead to an inhibition against gram-positive (staphylococcus aureus, listeria monocytogenes, and bacillus cereus) and gram-negative microbes (salmonella enteritidis and escherichia coli o157:h7). in the minced pork model, oligocs ... | 2014 | 24767083 |
| antimicrobial and antiinsectan phenolic metabolites of dalea searlsiae. | continued interest in the chemistry of dalea spp. led to investigation of dalea searlsiae, a plant native to areas of the western united states. methanol extractions of d. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans a-d (1-4) and known flavanones (5 and 6). known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. structure determination of pure compounds was accomplished primarily b ... | 2014 | 24761805 |
| affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag. | we recently reported that silica is deposited on the coat of bacillus cereus spores as a layer of nanometer-sized particles (hirota et al. 2010 j bacteriol 192: 111-116). gene disruption analysis revealed that the spore coat protein cotb1 mediates the accumulation of silica (our unpublished results). here, we report that b. cereus cotb1 (171 amino acids [aa]) and its c-terminal 14-aa region (corresponding to residues 158-171, designated cotb1p) show strong affinity for silica particles, with dis ... | 2014 | 24756322 |
| the phosphotransferase system gene ptsi in the endophytic bacterium bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot. | natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. in this study, we investigated the biocontrol properties of endophytic bacterium bacillus cereus strain 0-9, which was isolated from the root systems of healthy wheat varieties. the phosphotransferase system is a major regulator of carbohydrate metabolism in ba ... | 2014 | 24750250 |
| microwave-assisted synthesis of diverse pyrrolo[3,4-c]quinoline-1,3-diones and their antibacterial activities. | with the aim of developing a general and practical method for library production, a novel and efficient two-phase microwave-assisted cascade reaction between isatins and β-ketoamides in [bmim]bf4/toluene was developed for the synthesis of pyrrolo[3,4-c]quinoline-1,3-diones. the features of this methodology are, the use of microwave-assisted rapid synthesis, mild reaction conditions, high yields, operational simplicity, facile product separation, and recyclability. furthermore, the antibacterial ... | 2014 | 24749663 |
| isolation and structural elucidation of antioxidant peptides from oyster (saccostrea cucullata) protein hydrolysate. | protein derived from the oyster (saccostrea cucullata) was hydrolyzed using protease from bacillus cereus su12 for isolation of antioxidant peptides. the oyster hydrolysate exhibited a strong antioxidant potential in dpph (85.7±0.37%) followed by hydrogen peroxide radical scavenging activity (81.6±0.3%), hydroxyl radical-scavenging activity (79.32±0.6%), reducing power assay (2.63±0.2 od at 700nm). due to the high antioxidant potential, hydrolysate was fractionated in sephadex g-25 gel filtratio ... | 2014 | 24746143 |
| antimicrobial potential of bacteriocin producing lysinibacillus jx416856 against foodborne bacterial and fungal pathogens, isolated from fruits and vegetable waste. | in this study, antimicrobial potential, some probiotics properties and bacteriocin nature of lysinibacillus, isolated from fruits and vegetable waste were evaluated. for this, 125 lactobacillus isolates were tested against foodborne bacterial and fungal pathogens. among these, an isolated bacillus spp. showed significant aggregation-co-aggregation probiotics properties and potentially inhibits the foodborne gram positive microbial pathogens such as staphylococcus aureus, (22 mm zoi), staphylococ ... | 2014 | 24735603 |
| metabolomic analysis of cooperative adaptation between co-cultured bacillus cereus and ketogulonicigenium vulgare. | the cooperative adaptation of subcultivated bacillus cereus and ketogulonicigenium vulgare significantly increased the productivity of 2-keto-l-gulonic acid, the precursor of vitamin c. the mechanism of cooperative adaptation of the serial subcultivated b. cereus and k. vulgare was investigated in this study by culturing the two strains orthogonally on agar plates. it was found that the swarming distance of b. cereus along the trace of k. vulgare on the plate decreased after 150 days' subcultiva ... | 2014 | 24728527 |
| two unrelated episodes of bacillus cereus bacteremia in a neonatal intensive care unit. | | 2014 | 24725514 |
| antifungal and antibacterial metabolites from an endophytic aspergillus sp. associated with melia azedarach. | seven known metabolites, dianhydro-aurasperone c (1), isoaurasperone a (2), fonsecinone a (3), asperpyrone a (4), asperazine (5), rubrofusarin b (6) and (r)-3-hydroxybutanonitrile (7), were isolated from the culture of aspergillus sp. kj-9, a fungal endophyte isolated from melia azedarach and identified by spectroscopic methods. all isolates were evaluated in vitro against several phytopathogenic fungi (gibberella saubinetti, magnaporthe grisea, botrytis cinerea, colletotrichum gloeosporioides a ... | 2014 | 24708541 |
| antibacterial activities and in vitro anti-inflammatory (membrane stability) properties of methanolic extracts of gardenia coronaria leaves. | this work is carried out with gardenia coronaria leaves that belong to the family rubiaceae, which is a small-to-medium-sized but tall, deciduous tree, 7.6-9 m high on an average. leaves are used for the treatment of rheumatic pain and bronchitis. the leaf of the plant consists of coronalolide, coronalolic acid, coronalolide methyl ester, ethyl coronalolate acetate triterpenes (secocycloartanes), and so forth. methanol extract from the leaves of gardenia coronaria was completely screened for mem ... | 2014 | 24695677 |
| bioaugmentation treatment of mature landfill leachate by new isolated ammonia nitrogen and humic acid resistant microorganism. | the mature landfill leachate, which is characterized by a high concentration of ammonia nitrogen (nh3-n) and humic acid (ha), poses a challenge to biotreatment methods, due to the constituent toxicity and low biodegradable fraction of the organics. in this study, we applied bioaugmentation technology in landfill leachate degradation by introducing a domesticated nh3-n and ha resistant bacteria strain, which was identified as bacillus cereus (abbreviated as b. cereus jlu) and enterococcus casseli ... | 2014 | 24690637 |
| gene detection and toxin production evaluation of hemolysin bl of bacillus cereus isolated from milk and dairy products marketed in brazil. | bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. besides, it is a pathogen responsible for different types of food poisoning. one type of foodborne disease caused by b.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. one virulence factor for the diarrheal syndrome is the toxin hemolysin bl (hbl), a three-compone ... | 2013 | 24688511 |
| survival of foodborne pathogenic bacteria (bacillus cereus, escherichia coli o157:h7, salmonella enterica serovar typhimurium, staphylococcus aureus, and listeria monocytogenes) and bacillus cereus spores in fermented alcoholic beverages (beer and refined rice wine). | only limited information is available on the microbiological safety of fermented alcoholic beverages because it is still a common belief that such beverages do not provide a favorable environment for bacterial growth and survival. thus, in this study, we examined the survival of major foodborne pathogens and spores in fermented alcoholic beverages. foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica serovar typhimurium, and staphylococcus a ... | 2014 | 24674433 |
| effect of lead on growth, protein and biosorption capacity of bacillus cereus isolated from industrial effluent. | a bacterial strain (bacillus cereus) with the ability to grow under conditions of high concentrations of lead was isolated from the industrial effluent collected from peenya industrial area, bangalore. the effect of lead on growth, protein content and lead biosorption capacity of bacillus cereus was investigated. the results revealed that with increase in lead concentration (100, 200, 300, 400 and 500 mg i(-1)) there was a decrease in growth, protein content (10.6, 8.2, 6.7, 3.8 and 1.9 mg g(-1) ... | 2014 | 24665770 |
| chemistry and biological activities of tanacetum chiliophyllum var. oligocephalum extracts. | tanacetum chiliophyllum (fisch. & mey.) var. oligocephalum (d.c.) sosn. collected in turkey was subjected to phytochemical and biological evaluations in this study. pure compounds were obtained from ethyl acetate extracts of the stems of the plant material. structures of isolated compounds were determined using spectral methods. seven known flavones, i.e., 5-hydroxy-3',4',6,7-tetramethoxyflavone, eupatilin (6-hydroxyluteolin-6,3',4'-trimethylether), cirsimaritin (scuttellarin-6,7-dimethylether), ... | 2016 | 24645497 |
| green synthesis of silver nanoparticles using delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities. | green synthesis of silver nanoparticles (agnps) using aqueous root extract of delphinium denudatum (dd) by reduction of ag(+) ions from silver nitrate solution has been investigated. the synthesized ddagnps were characterized by using uv-vis spectroscopy, x-ray diffraction (xrd), field emission scanning electron microscope (fesem) and fourier transform infrared spectroscopy (ftir). the prepared ddagnps showed maximum absorbance at 416nm and particles were polydispersed in nature, spherical in sh ... | 2014 | 24632157 |
| a fullerene colloidal suspension stimulates the growth and denitrification ability of wastewater treatment sludge-derived bacteria. | fullerene (c60) is a nanoparticle that has been widely studied and applied in numerous commodities. however, there are concerns regarding its potential negative impact on the environment. a fullerene colloidal suspension (nc60) is known for its property of selectively inhibiting the growth of microorganisms. in this study, using denaturing gradient gel electrophoresis fingerprinting technology, we found that fullerene altered the structure of a sludge-derived microbial community. specifically, t ... | 2014 | 24630252 |
| diversity of pulsed-field gel electrophoresis patterns of cereulide-producing isolates of bacillus cereus and bacillus weihenstephanensis. | bacillus cereus is an important foodborne pathogen causing diarrhoea, emesis and in, rare cases, lethal poisonings. the emetic syndrome is caused by cereulide, a heat-stable toxin. originally considered as a rather homogenous group, the emetic strains have since been shown to display some diversity, including the existence of two clusters of mesophilic b. cereus and psychrotolerant b. weihenstephanensis. using pulsed-field gel electrophoresis (pfge) analysis, this research aimed to better unders ... | 2014 | 24627989 |
| association between neutropenia and brain abscess due to bacillus cereus bacteremia in patients with hematological malignancies. | | 2014 | 24625329 |
| comparative proteomic analysis of experimental evolution of the bacillus cereus-ketogulonicigenium vulgare co-culture. | the microbial co-culture system composing of ketogulonicigenium vulgare and bacillus cereus was widely adopted in industry for the production of 2-keto-gulonic acid (2-kga), the precursor of vitamin c. we found serial subcultivation of the co-culture could enhance the yield of 2-kga by 16% in comparison to that of the ancestral co-culture. to elucidate the evolutionary dynamics and interaction mechanisms of the two microbes, we performed itraq-based quantitative proteomic analyses of the pure cu ... | 2014 | 24619085 |
| isolation and characterization of chitinase-producing bacillus and paenibacillus strains from salted and fermented shrimp, acetes japonicus. | chitinases catalyze the conversion of chitin and are produced by a wide range of bacteria. the biological applications of these enzymes have been exploited in food and pharmaceutical industries. we isolated 2 halophilic chitinase-producing novel strains of bacteria-sch-1 and sch-2 from saeu-jeot, a traditional korean salted and fermented food made with shrimp (acetes japonicus). the isolated strains- sch-1 and sch-2 were gram-positive, rod-shaped, endospore-forming facultative anaerobes, with st ... | 2014 | 24611959 |
| quantification of the impact of single and multiple mild stresses on outgrowth heterogeneity of bacillus cereus spores. | outgrowth heterogeneity of bacterial spore populations complicates both prediction and efficient control of spore outgrowth. in this study, the impact of mild preservation stresses on outgrowth of bacillus cereus atcc 14579 spores was quantified during the first stages of outgrowth. heterogeneity in outgrowth of heat-treated (90°c for 10 min) and non-heat-treated germinated single spores to the maximum micro-colony stage of 256 cells was assessed by direct imaging on anopore strips, placed on bh ... | 2014 | 24607860 |
| crystal and solution structure analysis of fhud2 from staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-b. | iron acquisition is a central process for virtually all organisms. in staphylococcus aureus, fhud2 is a lipoprotein that is a high-affinity receptor for iron-bound hydroxamate siderophores. in this study, fhud2 was crystallized bound to ferrioxamine-b (fxb), and also in its ligand-free state; the latter structures are the first for hydroxamate-binding receptors within this protein family. the structure of the fhud2-fxb conformation shows that residues w197 and r199 from the c-terminal domain don ... | 2014 | 24606332 |
| de-hairing protease production by an isolated bacillus cereus strain at under solid-state fermentation using cow dung: biosynthesis and properties. | agro-industrial residues and cow dung were used as the substrate for the production of alkaline protease by bacillus cereus strain at. the bacterial strain bacillus cereus strain at produced a high level of protease using cow dung substrate (4813 ± 62 u g(-1)). physiological fermentation factors such as the incubation time (72 h), the ph (9), the moisture content (120%), and the inoculum level (6%) played a vital role in the enzyme bioprocess. the enzyme production improved with the supplementat ... | 2014 | 24596497 |
| characterization and genome analysis of the bacillus cereus-infecting bacteriophages bps10c and bps13. | due to the emergence of antibiotic-resistant strains, bacteriophages are considered to be an alternative approach for the control of pathogens. in this study, the bacteriophages bps10c and bps13 were isolated and characterized to investigate their ability to control food-borne pathogenic bacillus cereus. phage bps13 exhibited slightly higher host lysis activity compared with phage bps10c. in addition, phage bps13 exhibited greater stability under various ph and temperature conditions. to extend ... | 2014 | 24595561 |
| soy whey based medium for optimized phytase activity in saccharomyces cerevisiae mtcc 5421 and α-d-galactosidase and antibacterial activities in lactobacillus plantarum mtcc 5422 by response surface methodology. | soy whey based growth medium for yeast enabled the native isolate of saccharomyces cerevisiae mtcc 5421 to produce 198 u/ml of phytase activity against that of 135 u/ml in potato dextrose broth. in the same medium for lactic acid bacteria, the isolate of lactobacillus plantarum mtcc 5422 was able to elaborate α-d-galactosidase activity of 10.6 u/ml in comparison to 6 u/ml in lactobacillus mrs broth. using this formulated medium, a central composite experimental design based on 5 variables of 3 f ... | 2014 | 24587527 |
| seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter. | bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 217 clinical b. cereus isola ... | 2014 | 24584718 |
| provenance in bioinformatics workflows. | in this work, we used the prov-dm model to manage data provenance in workflows of genome projects. this provenance model allows the storage of details of one workflow execution, e.g., raw and produced data and computational tools, their versions and parameters. using this model, biologists can access details of one particular execution of a workflow, compare results produced by different executions, and plan new experiments more efficiently. in addition to this, a provenance simulator was create ... | 2013 | 24564294 |
| bioaccumulation characterization of cadmium by growing bacillus cereus rc-1 and its mechanism. | in an effort to explore the protective mechanism of growing bacillus cereus rc-1 against the toxicity of different cd(ii) concentrations, bacterial growth, cadmium consumption, surface interactions and intra- and extra-cellular cd(ii) contents were examined. cellular morphology and growth were evidently affected by the initial metal concentrations above 20 mg l(-1), according to the analysis of sem, afm, tem and uv spectrophotometer. surface complexation and electrostatic attraction played an im ... | 2014 | 24560281 |
| antimicrobial activity of acid-hydrolyzed citrus unshiu peel extract in milk. | citrus fruit (citrus unshiu) peels were extracted with hot water and then acid-hydrolyzed using hydrochloric acid. antimicrobial activities of acid-hydrolyzed citrus unshiu peel extract were evaluated against pathogenic bacteria, including bacillus cereus, staphylococcus aureus, and listeria monocytogenes. antilisterial effect was also determined by adding extracts at 1, 2, and 4% to whole, low-fat, and skim milk. the cell numbers of b. cereus, staph. aureus, and l. monocytogenes cultures treate ... | 2014 | 24534507 |
| colloidal polyaniline dispersions: antibacterial activity, cytotoxicity and neutrophil oxidative burst. | polyaniline colloids rank among promising application forms of this conducting polymer. cytotoxicity, antibacterial activity, and neutrophil oxidative burst tests were performed on cells treated with colloidal polyaniline dispersions. the antibacterial effect of colloidal polyaniline against gram-positive and gram-negative bacteria was most pronounced for bacillus cereus and escherichia coli, with a minimum inhibitory concentration of 3,500 μg ml(-1). the data recorded on human keratinocyte (hac ... | 2014 | 24534430 |
| cerecidins, novel lantibiotics from bacillus cereus with potent antimicrobial activity. | lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are widely produced by gram-positive bacteria, including many species of the bacillus group. in the present study, one novel gene cluster coding lantibiotic cerecidins was unveiled in bacillus cereus strain as 1.1846 through genomic mining and pcr screening. the designated cer locus is different from that of conventional class ii lantibiotics in that it included seven tandem precursor cera genes ... | 2014 | 24532070 |
| antibacterial potency of housefly larvae extract from sewage sludge through bioconversion. | use of the fly to convert sewage sludge into nutrient-rich soil conditioner and amendment is an attractive approach for sludge bioconversion. during this process, fecal coliforms, an indicating pathogen, in sludge were reduced to 5.3 x 10(2) most probable number/g dry solid from initial 3.32 x 10(6) mpn/g dry solid. it was also found that the extract of larvae grown in sludge during bioconversion have an observable inhibitory effect against bacteria compared to larvae grown in wheat bran as meas ... | 2013 | 24520734 |
| influence of probiotic bacterium bacillus cereus isolated from the gut of wild shrimp penaeus monodon in turn as a potent growth promoter and immune enhancer in p. monodon. | a probiotic bacterium isolated from the gut of wild shrimp penaeus monodon rendered maximum antagonistic activity against shrimp pathogens and was capable of producing extracellular enzymes. the probiotic bacterium was identified as bacillus cereus through 16s rrna sequencing. the lyophilized b. cereus was supplemented with shrimp basal diet at four different concentrations (0.1–0.4%/100 g feed) in d1–d4 diets. the viability of probiotic bacterium in the test diets was evaluated during the study ... | 2014 | 24516873 |
| influence of phenolic compounds of kangra tea [camellia sinensis (l) o kuntze] on bacterial pathogens and indigenous bacterial probiotics of western himalayas. | phenolic compounds of nutraceutical importance viz., catechins (c), (-)-epicatechin (ec), (-)-epigallocatechin (egc), (-)-epigallocatechin-3-gallate (egcg) and (-)-epicatechin-3-gallate (ecg) were estimated in fresh green tea shoots of camellia sinensis (l) o kuntze cultivar. the total polyphenols and total catechins were in the range of 219.90 to 317.81 and 140.83 to 271.39 g/kg, respectively in monthly samples of tea. the values of c, ec, egc, egcg and ecg in tea powders as analyzed through hi ... | 2013 | 24516437 |
| the caskr two-component system is required for the growth of mesophilic and psychrotolerant bacillus cereus strains at low temperatures. | the different strains of bacillus cereus can grow at temperatures covering a very diverse range. some b. cereus strains can grow in chilled food and consequently cause food poisoning. we have identified a new sensor/regulator mechanism involved in low-temperature b. cereus growth. construction of a mutant of this two-component system enabled us to show that this system, called caskr, is required for growth at the minimal temperature (tmin). caskr was also involved in optimal cold growth above tm ... | 2014 | 24509924 |
| antimicrobial and physical-mechanical properties of agar-based films incorporated with grapefruit seed extract. | the use of synthetic petroleum based packaging films caused serious environmental problems due to their difficulty in recycling and poor biodegradability. therefore, present study was aimed to develop natural biopolymer-based antimicrobial packaging films as an alternative for the synthetic packaging films. as a natural antimicrobial agent, grapefruit seed extract (gse) has been incorporated into agar to prepare antimicrobial packaging film. the films with different concentrations of gse were pr ... | 2014 | 24507339 |
| food-borne pathogens of animal origin-diagnosis, prevention, control and their zoonotic significance: a review. | the term food borne diseases or food-borne illnesses or more commonly food poisoning are used to denote gastrointestinal complications that occur following recent consumption of a particular food or drink. millions of people suffer worldwide every year and the situation is quiet grave in developing nations creating social and economic strain. the food borne pathogens include various bacteria viz., salmonella, campylobacter, escherichia coli, listeria monocytogenes, yersinia enterocolitica, staph ... | 2013 | 24506006 |
| vacuum distillation residue upgrading by an indigenous bacillus cereus. | biological processing of heavy fractions of crude oils offers less severe process conditions and higher selectivity for refining. biochemical processes are expected to be low demand energy processes and certainly ecofriendly. | 2013 | 24499629 |
| multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon). | the coastal aquaculture mainly shrimps constitute major export sector in bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. pcr based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. the objective of this study was to evaluate overall performance (sensitivity and specificity) of the multipl ... | 2013 | 24498789 |
| efficacy of commercial natural antimicrobials alone and in combinations against pathogenic and spoilage microorganisms. | microbial control strategies are needed in the food industry to prevent foodborne illnesses and outbreaks and prolong product shelf life. the aim of this study was to investigate and compare the efficacy of the commercial natural antimicrobials white mustard essential oil (wmeo), citrus flavonoid and acid blend (cfab), olive extract (oe), nisaplin (a compound containing nisin), and lauric arginate (lae) alone and in combinations against foodborne pathogens and spoilage microorganisms. mics of in ... | 2014 | 24490921 |
| global overview of the risk linked to the bacillus cereus group in the egg product industry: identification of food safety and food spoilage markers. | to evaluate the food safety and spoilage risks associated with psychrotrophic bacillus cereus group bacteria for the egg product industry and to search for relevant risk markers. | 2014 | 24484429 |
| expression, purification, and solubility optimization of peptidyl-trna hydrolase 1 from bacillus cereus. | peptidyl-trna hydrolase 1 cleaves the ester bond of peptidyl-trna thereby recycling peptidyl-trnas generated from premature termination of translation and expression of minigenes and short orfs. bacterial pth1 is essential, highly conserved, and has no essential eukaryotic homolog making it a good target for antibacterial development. herein we describe the cloning of pth1 gene from bacillus cereus as an n-terminal hexahistidine fusion protein. solubility was optimized for overexpression in esch ... | 2014 | 24480186 |
| spectroscopy and solvatochromism studies along with antioxidant and antibacterial activities investigation of azo-azomethine compounds 2-(2-hydroxyphenylimino)methyl)-4-phenyldiazenyl)phenol. | the azo-azomethine compounds 2-(2-hydroxyphenylimino)methyl)-4-phenyldiazenyl)phenol (2a-d) were prepared from the reaction of 2-aminophenol with 2-hydroxy-5-(aryldiazenyl)benzaldehyde (1a-d). the structures of all compounds were then characterized by elemental analysis, mass, infrared, uv-vis, (1)h and (13)c nmr spectroscopy. the electronic absorption spectra indicated enol-keto tautomeric and positive solvatochromism in compounds (2a-d), which is dependent on the substitution, nature of solven ... | 2014 | 24480116 |
| genome sequences of three novel bacillus cereus bacteriophages. | the bacillus cereus group is an assemblage of highly related firmicute bacteria that cause a variety of diseases in animals, including insects and humans. we announce three high-quality, complete genome sequences of bacteriophages we isolated from soil samples taken at the bases of fruit trees in utah county, utah. while two of the phages (shanette and jl) are highly related myoviruses, the bacteriophage basilisk is a siphovirus. | 2014 | 24459255 |
| nisin a extends the shelf life of high-fat chilled dairy dessert, a milk-based pudding. | the aims of this study were to evaluate the effectiveness of nisin a to control the growth of spore-forming bacteria, bacillus and paenibacillus, in chilled high-fat, milk pudding and to reduce heat treatment to improve aroma and flavour. | 2014 | 24450783 |
| brown seaweed (saccharina japonica) as an edible natural delivery matrix for allyl isothiocyanate inhibiting food-borne bacteria. | the edible, brown seaweed saccharina japonica was prepared as powder in the size range 500-900 μm for the desorption release of allyl isothiocyanate (aitc). powders were used as raw (containing lipids) and as de-oiled, where the lipid was removed. in general, de-oiled powders adsorbed larger masses of aitc after vapour or solution contact. mass adsorbed due to solution contact exceeded vapour contact. larger particles adsorbed more than smaller particles. no chemical bonding between aitc and the ... | 2014 | 24444900 |
| purification of chitinase/chitosanase from bacillus cereus and discovery of an enzyme inhibitor. | a chitinase and a chitosanase were induced from a squid pen powder (spp)-containing medium of bacillus cereus tku030 and purified by precipitation with ammonium sulphate and combined column chromatography. the purified chitinase and chitosanase exhibited optimum activity at 60 °c, ph 5-6 and 40 °c, ph 4, respectively. the chitinase and chitosanase were stable at 25-60 °c, ph 4-7 and 25-50 °c, ph 3-7, respectively. the chitinase and chitosanase showed the highest activity toward β-chitin and 60% ... | 2014 | 24444885 |
| surfactin restores and enhances swarming motility under heavy metal stress. | the present work reports the importance of lipopeptide biosurfactant on swarming motility of multi-metal resistant (mmr) bacterium under heavy metal stress. the mmr bacteria strain cm100b, identified as bacillus cereus, was isolated from the coal mine sample. the strain was able to grow and reduce several metals namely cd(2+), co(2+), cu(2+), ni(2+), mn(2+) and pb(2+) ions which are common environmental pollutants. presence of toxic heavy metal ions in the swarming medium significantly altered t ... | 2014 | 24441179 |
| hydrothermal synthesis of copper based nanoparticles: antimicrobial screening and interaction with dna. | copper based nanoparticles (cu-based nps) of different compositions and sizes have been hydrothermally synthesized by varying the reaction time in the presence of the biocompatible surfactants polyoxyethylene (20) sorbitan laurate (tween 20) and polyethylene glycol 8000 (peg 8000). effective control of the above synthetic parameters gave rise to cu, cu2o and cu/cu2o nps of 10-44 nm. the antibacterial activity of the nps was screened against gram-positive (bacillus subtilis, bacillus cereus, stap ... | 2014 | 24441110 |
| isolation of bacillus cytotoxicus from various commercial potato products. | bacillus (b.) cytotoxicus is a newly described thermotolerant member of the bacillus cereus group. this potential foodborne pathogen had so far only been isolated from vegetable products, including mashed potatoes. here we report the detection of b. cytotoxicus in a variety of potato products taken on retail level or from catering establishments (n=151). identification of isolates as b. cytotoxicus was performed after enrichment at 50°c, followed by differentiation using fourier transform-infrar ... | 2014 | 24440535 |
| impact of donor arm cleaning with different aseptic solutions for prevention of contamination in blood bags. | transfusion associated sepsis cases are encountered occasionally and bacterial transmission remains the major cause. the goal of our study was to compare the efficacy of disinfectants in phlebotomy site preparation. after selection of donor the antecubital fossa area of the arm was disinfected with different types of disinfectants namely sprit (70% isopropyl alcohol), povidone iodine (0.5% w/v available iodine in distilled water), savlon (1.5% v/v chlorhexidine gluconate solution and 3.0% cetrim ... | 2013 | 24426327 |
| microbial treatment for prevention and removal of paraffin deposition on the walls of crude pipelines. | two bacterial strains, paraffin removal strain and biosurfactant-producing strain, named bhj-1 and qfl-1, were isolated from oil production wells in daqing oilfield of china. they were subsequently identified as bacillus cereus qau68 and bacillus subtilis xccx, respectively. as an indicator of the degradation paraffin, the inoculum concentration of bhj-1 and qfl-1 were added in different proportions, the optimum proportion was 5:2. in this proportion the degradation rate of paraffin could reach ... | 2013 | 24426154 |
| impact of lactococcus lactis spp. lactis bio adhesion on pathogenic bacillus cereus biofilm on silicone flowing system. | bacillus cereus is a food pathogen that can attach on most of the surfaces and form biofilms, which facilitate the persistence and resistance toward antimicrobials. the aims of this study were (i) to characterize the structural dynamics of b. cereus sessile growth in two nutritional environments (with or without a nutrient flow), and (ii) to evaluate the impact of bio adhesion of lactococcus lactis on b. cereus biofilm. significantly greater biofilm volume and thickness were observed under dynam ... | 2013 | 24426121 |
| biosynthesis and characterization of mercury sulphide nanoparticles produced by bacillus cereus mrs-1. | mercury is a highly toxic heavy metal accumulated in the environment, which can be detoxified by reducing hg2+ to non toxic form. bacteria resistant to toxic metals and capable of converting them into non toxic forms have a direct application in the bioremediation of contaminated sites. in this study, mercury resistant strain bacillus cereus mrs-1 was isolated from electroplating industrial effluent. this strain exhibited the ability to convert mercury into extracellular sulphide nanoparticles o ... | 2013 | 24416933 |
| [clinical characteristics and resistance of wound surface infections causing by bacillus cereus in the department of orthopaedics]. | to study clinical characteristics and resistance of wound surface infections, so as to guide clinical diagnosis and rational administration. | 2013 | 24416908 |
| genome sequence of bacillus cereus strain lct-bc235, carried by the shenzhou viii spacecraft. | in order to explore the effect of space environments on bacillus cereus, we determined the draft genome sequence of a b. cereus strain, lct-bc235, which was isolated after space flight. | 2014 | 24407625 |
| effect of temperatures on the growth, toxin production, and heat resistance of bacillus cereus in cooked rice. | bacillus cereus is capable of producing enterotoxin and emetic toxin, and bacillus foodborne illnesses occur due to the consumption of food contaminated with endospores. the objectives of this study were to investigate the growth and toxin production of b. cereus in cooked rice and to determine the effect of temperature on toxin destruction. cooked rice inoculated with b. cereus was stored at 15, 25, 35, and 45°c or treated at 80, 90, and 100°c. the results indicated that emetic toxin was produc ... | 2014 | 24404779 |
| effects of methanol extract of wedelia chinensis osbeck (asteraceae) leaves against pathogenic bacteria with emphasise on bacillus cereus. | the antibacterial activity of the methanol extract of wedelia chinensis leave was studied and tested against three pathogenic gram positive bacteria (bacillus cereus, b. subtilis and stapylococcus aureus) and three pathogenic gram negative bacteria (escherichia coli, proteus rettgeri and pseudomonas aeruginosa) by the disk diffusion assay and broth dilution methods. the extract exhibited favourable antibacterial activity against the bacterial cells but was more potent against gram positive bacte ... | 2013 | 24403653 |
| trifolium pratense l. as a potential natural antioxidant. | the essential oils of three different growth stages of trifolium pratense l. (tp1, tp2 and tp3) were investigated by gas chromatography-mass spectrometry and tested for their antioxidant and antimicrobial activities. the highest content of volatile compounds was found in the essential oil sample tp1, where terpenes such as β-myrcene (4.55%), p-cymene (3.59%), limonene (0.86%), tetrahydroionone (1.56%) were highlighted due to their biological activity. the antioxidant activity was determined by f ... | 2014 | 24402202 |
| antimicrobial activity of traditional medicinal plants from ankober district, north shewa zone, amhara region, ethiopia. | traditional medicinal plants have long been used in ethiopia to treat human and livestock ailments. despite a well-documented rich tradition of medicinal plant use in the country, their direct antimicrobial effects are still poorly known. | 2014 | 24392738 |
| dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of cr6+ from real tannery effluent employing indigenous bacillus cereus isolate. | a native bacillus cereus isolate has been employed, for the first time, for simultaneous decolorization, dechlorination of chloroorganics, and cr(6+) remediation from the real tannery effluent. most of the physicochemical variables in 3:1 diluted effluent were well above the standard prescribed limits, which decreased substantially upon microbial treatment. the extent of bioremediation was better in diluted (3:1) as compared to undiluted effluent supplemented with nutrients and augmented with b. ... | 2014 | 24390198 |
| draft genome sequence of bacillus cereus lct-bc25, isolated from space flight. | bacillus cereus strain lct-bc25, which was carried by the shenzhou viii spacecraft, traveled in space for about 398 h. to investigate the response of b. cereus to space environments, we determined the genome sequence of b. cereus strain lct-bc25, which was isolated after space flight. | 2014 | 24385570 |
| differential regulation and activity against oxidative stress of dps proteins in bacillus cereus. | in this study, the sequence similarity, structure, ferroxidase activity and efficacy in antagonizing oxidative stress of three dps-like proteins, dps1, dps2 and dps3, encoded by bacillus cereus were comparatively analyzed. the three dps-like proteins are homologous to other bacterial dps proteins that exhibit ferroxidase activity. both dps1 and dps2 have a typical dps spherical structure, but dps3 has a unique filamentous structure. several dps mutant strains were generated to investigate the fu ... | 2013 | 24383075 |
| antibacterial activity of different natural honeys from transylvania, romania. | honey is used in food industry and medicine due to its nutritive, therapeutic and dietetic qualities. the microbiological characteristics of 10 unpasteurized honey samples of known origin, collected from transylvania beekeepers (romania) were determined. the antibacterial activity of these types of honey against escherichia coli, staphylococcus aureus, staphylococcus epidermidis, salmonella enteritidis, salmonella anatum, salmonella choleraesuis, bacillus cereus, bacillus subtilis subsp. spizize ... | 2014 | 24380618 |
| synthesis of novel 2-(substituted amino)alkylthiopyrimidin-4(3h)-ones as potential antimicrobial agents. | 5-alkyl-6-(substituted benzyl)-2-thiouracils 3a,c were reacted with (2-chloroethyl) diethylamine hydrochloride to afford the corresponding 2-(2-diethylamino)ethylthiopyrimidin- 4(3h)-ones 4a,b. reaction of 3a-c with n-(2-chloroethyl)pyrrolidine hydrochloride and/or n-(2-chloroethyl)piperidine hydrochloride gave the corresponding 2-[2-(pyrrolidin-1-yl)ethyl]-thiopyrimidin-4(3h)-ones 5a-c and 2-[2-(piperidin-1-yl)ethyl]thiopyrimidin-4(3h)-ones 6a,b, respectively. treatment of 3a-d with n-(2-chloro ... | 2013 | 24378967 |
| synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications. | silver has long been advocated as an effective antimicrobial. however, toxicity issues with silver have led to limited use of silver in nanoform, especially for food preservation. with the aim of exploring combinatorial options that could increase the antibacterial potency of silver nanoparticles and reduce the effective dosage of silver, we evaluated the extent of synergy that a combination of silver nanoparticles and an essential oil representative (cinnamaldehyde) could offer. a battery of gr ... | 2013 | 24376352 |
| in-vitro anticancer and antimicrobial activities of plga/silver nanofiber composites prepared by electrospinning. | in the present work, a series of 0, 1 and 7 wt% silver nano-particles (ag nps) incorporated poly lactic-co-glycolic acid (plga) nano-fibers were synthesized by the electrospinning process. the plga/ag nano-fibers sheets were characterized using sem, tem and dsc analyses. the three synthesized plga/silver nano-fiber composites were screened for anticancer activity against liver cancer cell line using mtt and ldh assays. the anticancer activity of plga nano-fibers showed a remarkable improvement d ... | 2014 | 24375170 |
| spore germination mediated by bacillus megaterium qm b1551 slel and ypeb. | previous work demonstrated that bacillus megaterium qm b1551 spores that are null for the sleb and cwlj genes, which encode cortex-lytic enzymes (cles), either of which is required for efficient cortex hydrolysis in bacillus spores, could germinate efficiently when complemented with a plasmid-borne copy of ypeb plus the nonlytic portion of sleb encoding the n-terminal domain of sleb (sleb(n)). the current study demonstrates that the defective germination phenotype of b. megaterium sleb cwlj spor ... | 2014 | 24375103 |
| antimicrobial effectiveness of silicone oil, heavy silicone oil and perfluorodecaline against bacillus cereus. | the purpose of this work is to study the effectiveness of silicone oil, heavy silicone oil and perfluorodecaline against bacillus cereus. for this we tested the antimicrobial activities of three intraocularly used liquids--silicone oil (siluron 1000), heavy silicone oil (densiron-68) and perfluorodecaline (f-decalin)--against b. cereus (nctc 9946). the antimicrobial effects of silicone oil, heavy silicone oil and perfluorodecaline were determined by the growing capability of the microorganism. w ... | 2014 | 24375026 |
| impact of ag and al₂o₃ nanoparticles on soil organisms: in vitro and soil experiments. | in vitro analyses were conducted to assess the impact of al2o3 and ag nanoparticles on two common soil bacteria, bacillus cereus and pseudomonas stutzeri. al2o3 nanoparticles did not show significant toxicity at any dose or time assayed, whereas exposure to 5 mg l(-1) ag nanoparticles for 48 h caused bactericidal effects. moreover, alterations at the morphological level were observed by transmission electron microscopy (tem); ag but not al2o3 nanoparticles evoked the entrance of b. cereus cells ... | 2014 | 24374587 |
| haplophytin b from maclurodendron porteri. | an alkaloid from maclurodendron porteri has been isolated and characterized. extraction process was conducted by acid-base extraction method followed by column chromatography. the structure was established by nuclear magnetic resonance spectroscopy and mass spectrometry. the compound was identified as haplophytin b which occurs commonly in the rutaceae family. however, this is the first time this alkaloid was isolated and reported from the species. the compound showed no inhibition against staph ... | 2014 | 24374446 |
| psp: rapid identification of orthologous coding genes under positive selection across multiple closely related prokaryotic genomes. | with genomic sequences of many closely related bacterial strains made available by deep sequencing, it is now possible to investigate trends in prokaryotic microevolution. positive selection is a sub-process of microevolution, in which a particular mutation is favored, causing the allele frequency to continuously shift in one direction. wide scanning of prokaryotic genomes has shown that positive selection at the molecular level is much more frequent than expected. genes with significant positiv ... | 2013 | 24373418 |
| identification of multidrug-resistant bacteria and bacillus cereus from healthcare workers and environmental surfaces in a hospital. | | 2014 | 28156280 |
| simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in low-fatted milk by multiplex pcr. | a rapid and specific pcr assay for the simultaneous detection of listeria monocytogenes, escherichia coli o157:h7, bacillus cereus, salmonella spp., and staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. multiplex pcr developed in this study produced only acta, flic, hbl, inva, iles amplicons, but did not produce any non-specific amplicon. the primer sets successfully amplified the target genes in the multiplex pcr without any non-specific or a ... | 2014 | 26761507 |
| detection of emetic bacillus cereus by real-time pcr in foods. | the simplex real-time pcr assays based on the taqman probe and sybr green i， which target cereulide synthetase genes (ces genes) were used for rapidly, reliably and sensitively identifying the emetic strains from among bacillus cereus strains isolated from different sources. only the emetic strains showed positive reactions to the real-time pcr assays, but all examined strains of diarrheal b. cereus, other bacillus species and other gram positive and negative bacteria gave negative results. the ... | 2013 | 24366630 |
| genetic determinants of antimicrobial resistance in gram positive bacteria from organic foods. | bacterial biocide resistance is becoming a matter of concern. in the present study, a collection of biocide-resistant, gram-positive bacteria from organic foods (including 11 isolates from genus bacillus, 25 from enterococcus and 10 from staphylococcus) were analyzed for genes associated to biocide resistance efflux pumps and antibiotic resistance. the only qac-genes detected were qaca/b (one bacillus cereus isolate) and smr (one b. cereus and two staphylococcus saprophyticus isolates). efflux p ... | 2014 | 24361832 |
| a new bis-labdanic diterpene from the rhizomes of alpinia pahangensis. | the rhizomes of alpinia pahangensis yielded a new bis-labdanic diterpene for which the name pahangensin c (1) was proposed along with twelve known analogues (2-13). the structure of 1 was elucidated via spectroscopic methods including 1d and 2d nmr techniques and lcms-it-tof analysis. compounds 2 and 12 were isolated for the first time from the genus alpinia. this is the second occurrence of compounds 2 and 12 in the zingiberaceae family. selected analogues exhibited moderate to strong inhibitor ... | 2013 | 24356874 |
| an alkenylresorcinol derivative from hakea sericea fruits and their antimicrobial activity. | hakea sericea has been introduced to portugal for ornamental purposes. the phytochemical composition and the antioxidant, antibacterial, antibiofilm and cytotoxic properties of this shrub species have been previously reported. the present work describes the bioassay-guided fractionation of the crude methanolic extract of h. sericea fruits and the isolation of 9-(3,5-dihydroxy-4-methylphenyl)nona-3(z)-enoic acid. the structure of this new compound was established by one- and two-dimensional nmr a ... | 2013 | 24354201 |
| lipids, proteins, phenolic composition, antioxidant and antibacterial activities of seeds of peanuts (arachis hypogaea l) cultivated in tunisia. | fatty acid composition of peanut seed oil in four varieties cultivated in tunisia showed that linoleic (c18:2), oleic (c18:1) and palmitic (c16) acids account for more than 84% for chounfakhi and massriya and for more than 85% of the total fatty acids of trabilsia and sinya seed oil respectively. seed oil contents were significantly different (p ≤ 0.05) and did not exceed 48%. the study of total phenolics revealed that chounfakhi contained more total phenolics (2.1 mg gae/g dw), followed by the ... | 2013 | 24346073 |
| involvement of alanine racemase in germination of bacillus cereus spores lacking an intact exosporium. | the l-alanine mediated germination of food isolated bacillus cereus dsa 1 spores, which lacked an intact exosporium, increased in the presence of d-cycloserine (dcs), which is an alanine racemase (alr) inhibitor, reflecting the activity of the alr enzyme, capable of converting l-alanine to the germination inhibitor d-alanine. proteomic analysis of the alkaline extracts of the spore proteins, which include exosporium and coat proteins, confirmed that alr was present in the b. cereus dsa 1 spores ... | 2014 | 24346000 |
| purification of a thermostable chitinase from bacillus cereus by chitin affinity and its application in microbial community changes in soil. | a thermostable chitinase was purified by chitin affinity from the culture supernatant of bacillus cereus tku028 with shrimp head powder (shp) as the sole carbon/nitrogen source. tku028 chitinase was purified using a one-step affinity adsorbent system, and the molecular mass of tku028 chitinase (approximately 40 kda) was then determined using sds-page. the enzyme was stable for 60 min at temperatures below 60 °c and stable over a broad ph range of 4-9 for 60 min. in addition, the temporal changes ... | 2014 | 24342954 |
| bio-augmentation as a tool for improving the modified sequencing batch biofilm reactor. | biological treatment of domestic sewage was accomplished in a pilot-scale modified sequencing batch biofilm reactor (sbbr) bio-augmented with consortium of 5 strains of indigenous bacteria (genus pseudomonas and bacillus). the reactor consisted of fibrous filler in the upper and ceramsite filter media in the bottom. it demonstrated to have a short hydraulic residence time (hrt) for 10 h and good quality effluent to cope with low c/n ratio domestic wastewater. the biofilm attached fibrous fillers ... | 2014 | 24342171 |
| new hydrazones with pyrazolone structure: synthesis, characterization and biological evaluation. | to design new hydrazones with pyrazolone structure with improved biological properties. | 2013 | 24340543 |