| screening dogs in ontario for borrelia burgdorferi and ehrlichia canis should be selective rather than routine. | | 2007 | 17824154 |
| [tick bites, lyme disease and early summer meningoencephalitis. current indications concerning infection risks, prevention and therapy]. | | 2007 | 17844683 |
| delineation of species-specific binding properties of the cspz protein (bbh06) of lyme disease spirochetes: evidence for new contributions to the pathogenesis of borrelia spp. | borrelia burgdorferi cspz (tigr open reading frame designation, bbh06) is part of a functionally related group of proteins that bind one or more members of the factor h (fh) protein family. in this report we assess the conservation, distribution, properties, and ligand binding abilities of cspz from the three main borrelia species associated with lyme disease infections in humans. cspz (also referred to as bbcrasp-2 in the literature) was found to be highly conserved at the intraspecies level bu ... | 2007 | 17846117 |
| ecological havoc, the rise of white-tailed deer, and the emergence of amblyomma americanum-associated zoonoses in the united states. | two infectious diseases, and one presumably infectious disease, each vectored by or associated with the bite of the lone star tick (amblyomma americanum), were identified and characterized by clinicians and scientists in the united states during the 1980s and 1990s. these three conditions-human monocytic (or monocytotropic) ehrlichiosis (hme), ehrlichia ewingii ehrlichiosis, and southern tick-associated rash illness (stari)-undoubtedly existed in the united states prior to this time. however, th ... | 2007 | 17848069 |
| fibrinolysis and host response in bacterial infections. | the plasminogen activation system is part of the fibrinolysis which is tightly regulated and protected against dysfunction by various activators and inhibitors. however, microorganisms including bacteria, fungi and also parasites have been proven to interact in a specific manner with components of the fibrinolytic pathways. pathogenic bacteria are capable to subvert the function of proteases, activators or inhibitors for their own benefits including dissemination within the host and evasion of h ... | 2007 | 17849039 |
| [diagnostic aspects of borrelia-infections in dogs]. | this paper discusses the problem of diagnosing borreliosis (lyme disease) in dogs. a prospective cohort study in the kempen district, a known borrelia focus in the netherlands, showed that dogs with the presumptive symptoms of borreliosis, episodic malaise and lameness, had significantly higher and longer lasting anti-borrelia igg titers than asymptomatic dogs. a small part of these dogs also had antibodies directed against the ir6 (c6) antigen which indicates persistent active borrelia infectio ... | 2007 | 17849909 |
| seroprevalence of rickettsia spp. infection among tick-bitten patients and blood donors in sweden. | serum samples from 236 swedish patients with symptoms of infectious disease appearing after a tick bite were analysed for the presence of antibodies to rickettsia helvetica, the only rickettsial species so far isolated from ticks in sweden. of these subjects, 137 had tested seropositive for borrelia burgdorferi. for control purposes, sera from 161 healthy blood donors were examined. a total of 10/397 samples (2.6%) showed igg-antibodies to r. helvetica at or above a titre of 1/80 as cut-off. 6/1 ... | 2008 | 17852905 |
| regulation of expression of the fibronectin-binding protein bbk32 in borrelia burgdorferi. | the bbk32 protein binds to host extracellular ligand fibronectin and contributes to the pathogenesis of borrelia burgdorferi. here we showed that expression of the bbk32 gene is influenced by multiple environmental factors and that its regulation is governed by the response regulator rrp2 and rpon-rpos (sigma(54)-sigma(s)) sigma cascade in b. burgdorferi. | 2007 | 17873053 |
| serum reactivity against borrelia burgdorferi ospa in patients with rheumatoid arthritis. | lyme arthritis and rheumatoid arthritis share common clinical features and synovial histology. it is unclear whether they also share similar pathogenesis. previous studies have shown that the severity and duration of lyme arthritis correlate directly with serum concentrations of antibody against outer surface protein a (ospa) of the causative pathogen borrelia burgdorferi. we tested the sera of 68 subjects with rheumatoid arthritis, 147 subjects with other autoimmune diseases, and 44 healthy sub ... | 2007 | 17881508 |
| feeding by the tick, ixodes scapularis, causes cd4(+) t cells responding to cognate antigen to develop the capacity to express il-4. | effects of tick feeding on an early antigen-specific t cell response were studied by monitoring a clonotypic population of adoptively transferred t cell receptor (tcr) transgenic cd4 cells responding to a tick-associated antigen. when recipient mice were infested with pathogen-free ixodes scapularis nymphs several days prior to t cell transfer and intradermal injection of soluble cognate antigen at the feeding site, the clonotypic cd4 cells gained the ability to express the th2 effector cytokine ... | 2007 | 17883452 |
| antigen biochips verify and extend the scope of antibody detection in lyme borreliosis. | the antibody response of serum igm and igg of patients with neuroborreliosis and erythema migrans of lyme borreliosis (lb) was examined against a 41-kda flagellar antigen and an 8-mer synthetic ospc8 peptide (vaespkkp) derived from the c-terminus of outer surface protein c (ospc) from borrelia garinii. we developed a streptavidin-modified biochip-based immunodiagnosis and compared it with conventional methods such as enzyme-linked immunosorbent assay (elisa) and western blot (wb). the diagnostic ... | 2007 | 17888607 |
| development of hepatitis b virus capsids into a whole-chain protein antigen display platform: new particulate lyme disease vaccines. | the immunogenicity of peptides and small protein fragments can be considerably enhanced by their presentation on particulate carriers such as capsid-like particles (clps) from hepatitis b virus (hbv). hbv clps are icosahedral nanoparticles formed by 90 or 120 core protein dimers. insertions into the immunodominant c/e1 b cell epitope, a surface-exposed loop on the hbv capsid protein, are especially immunogenic. here we investigated whether the hbv core protein can be exploited as a vaccine carri ... | 2008 | 17888729 |
| hypothesized role of galactocerebroside and nkt cells in the etiology of multiple sclerosis. | according to the molecular mimicry theory, multiple sclerosis (ms) develops when the immune system mistakenly attacks a component of the myelin sheath that is structurally similar to a foreign epitope. the glycolipid galactocerebroside (galc) is a major component of myelin. as lipids comprise between 70% and 85% of myelin, glycolipids should be investigated as candidate autoantigens in ms. galc displays broad structural similarities to the borrelia burgdorferi glycolipid antigen bbgl-2 and to th ... | 2008 | 17889444 |
| [lyme arthritis]. | lyme disease is a multi-system inflammatory disease caused by spirochetes of the borrelia burgdorferi. among multiplicity manifestations of lyme disease, musculoskeletal symptoms also occur. in the following article authors present epidemiology of lyme disease, pathogenesis of antibiotic-refractory lyme arthritis, symptoms, diagnosis and management of lyme arthritis based on recent guidelines prepared by an expert panel of the infectious diseases society of america. | 2007 | 17892043 |
| immunity in borreliosis with special emphasis on the role of complement. | | 2007 | 17892213 |
| comparison of different borrelia burgdorferi sensu lato strains for detection of immune response in patients with erythema migrans. | the aim of the present study was to establish which combination of serological method and borrelia strain used as an antigen would provide the most appropriate demonstration of borrelial infection in patients with eythema migrans residing in slovenia. four different strains were chosen as antigens: two strains of b. afzelii and two strains of b. garinii which differed in their expression of the outer proteins ospa, ospb and ospc. each individual strain was used as antigen in immunofluorescence t ... | 2008 | 17892971 |
| borrelia lusitaniae ospa gene heterogeneity in mediterranean basin area. | in this study, borrelia lusitaniae dna extracted from ticks and lizards was used to amplify the outer surface protein a (ospa) gene in order to increase knowledge about sequence variability in the mediterranean basin area, to better understand how borrelia lusitaniae has evolved and how its distribution has expanded. phylogenetic trees including italian and reference sequences showed a clear separation of b. lusitaniae ospa strains in two different major clades. north african isolates form a cla ... | 2007 | 17896068 |
| identification of salp15 homologues in ixodes ricinus ticks. | the 15-kda ixodes scapularis salivary gland protein salp15 protects borrelia burgdorferi sensu stricto from antibody-mediated killing and facilitates infection of the mammalian host. in addition, salp 15 has been shown to inhibit t-cell activation. we determined whether ixodes ricinus, the major vector for lyme borreliosis in western europe, also express salp15-related genes. we show that engorged i. ricinus express salp15 and we have identified three salp15 homologues within these ticks by reve ... | 2007 | 17896872 |
| release of latent clya cytolysin from escherichia coli mediated by a bacteriophage-associated putative holin (blya) from borrelia burgdorferi. | introduction of the borrelia burgdorferi blyab locus into escherichia coli has recently been reported to cause a hemolytic phenotype that is dependent on the e. coli clya (hlye, shea) gene (a cytolysin gene present in many e. coli strains, including e. coli k-12, which is repressed under standard in vitro growth conditions). the blya gene product has been suggested to be a prophage-encoded holin, but the processes triggered in e. coli by the expression of blya and/or blyb, which lead to the hemo ... | 2008 | 17897882 |
| anaplasma phagocytophilum-borrelia burgdorferi coinfection enhances chemokine, cytokine, and matrix metalloprotease expression by human brain microvascular endothelial cells. | borrelia burgdorferi and anaplasma phagocytophilum coinfect and are transmitted by ixodes species ticks. clinical indicators suggest that a. phagocytophilum coinfection contributes to the severity, dissemination, and, possibly, sequelae of lyme disease. previous in vitro studies showed that spirochete penetration through human brain microvascular endothelial cells of the blood-brain barrier is facilitated by endothelial cell-derived matrix metalloproteases (mmps). a. phagocytophilum-infected neu ... | 2007 | 17898182 |
| [is it useful in case of a tick bite to give one oral dose of 200mg doxycycline to prevent borrelia burgdorferi infection?]. | | 2007 | 17902565 |
| evidence of a conjugal erythromycin resistance element in the lyme disease spirochete borrelia burgdorferi. | we report the identification of isolates of borrelia burgdorferi strain b31 that exhibit an unusual macrolide-lincosamide (ml) or macrolide-lincosamide-streptogramin a (mls(a)) antibiotic resistance pattern. low-passage isolates were resistant to high levels (>100 microg/ml) of erythromycin, spiramycin and the lincosamides but were sensitive to dalfopristin, an analogue of streptogramin b. interestingly, the high-passage erythromycin-resistant strain b31 was resistant to quinupristin, an analogu ... | 2007 | 17905571 |
| [total atrioventricular block following a tick bite]. | a 40-year-old man was referred to the cardiology outpatient clinic with dizziness, palpitations and shortness of breath. he remembered being bitten by a tick two to three years previously, but had not noticed a characteristic skin rash. the ecg showed a prominent first degree atrioventricular (av) block and ambulatory electrocardiographic monitoring showed an intermittent complete av block. a definitive pacemaker was implanted. antibodies to borrelia were found. the patient was treated with ceft ... | 2007 | 17907546 |
| [role of various ixodes ticks as vectors of the causative agents of tick-borne encephalitis and borrelioses in the orenburg region]. | | 2007 | 17912831 |
| a critical appraisal of "chronic lyme disease". | | 2007 | 17914043 |
| molecular identification of bloodmeal source in ixodes ricinus ticks using 12s rdna as a genetic marker. | we developed an efficient molecular method for the identification of the bloodmeal sources in the tick ixodes ricinus (l.), the european vector of the agents of lyme borreliosis and tick-borne encephalitis. an approximately 145-bp orthologous fragment of the vertebrate mitochondrial 12s rdna was used as a molecular marker to discriminate host vertebrate species. the method consists of a single run polymerase chain reaction amplification of the 12s rdna molecular marker by using nondegenerate pri ... | 2007 | 17915521 |
| genetic basis for retention of a critical virulence plasmid of borrelia burgdorferi. | the genome of borrelia burgdorferi is composed of one linear chromosome and approximately 20 linear and circular plasmids. although some plasmids are required by b. burgdorferi in vivo, most plasmids are dispensable for growth in vitro. however, circular plasmid (cp) 26 is present in all natural isolates and has never been lost during in vitro growth. this plasmid carries ospc, which is critical for mammalian infection. we previously showed that cp26 encodes essential functions, including the te ... | 2007 | 17919281 |
| macromolecular crowding increases structural content of folded proteins. | here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical vlse and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. the structural content of flavodoxin and vlse is enhanced by 33% and 70%, respectively, in 400 mg/ml ficoll 70 (ph 7, 20 degrees c) and correlates with higher protein-thermal stability. in the same ficoll range, there are only small effects on the unfolded-state structure ... | 2007 | 17919600 |
| an octavalent lyme disease vaccine induces antibodies that recognize all incorporated ospc type-specific sequences. | lyme disease is the most common vector-borne disease in north america and europe and, if untreated, has significant arthritic, cardiac, dermatological and neurological sequelae. there is no currently available human lyme disease vaccine. outer surface protein c, because of its antigenicity, protective ability, and expression characteristics has emerged as a promising second generation vaccine candidate; however, significant sequence heterogeneity has impeded its development. analyses of ospc seq ... | 2007 | 17921702 |
| expression of the recombinant bacterial outer surface protein a in tobacco chloroplasts leads to thylakoid localization and loss of photosynthesis. | bacterial lipoproteins play crucial roles in host-pathogen interactions and pathogenesis and are important targets for the immune system. a prominent example is the outer surface protein a (ospa) of borrelia burgdorferi, which has been efficiently used as a vaccine for the prevention of lyme disease. in a previous study, ospa could be produced in tobacco chloroplasts in a lipidated and immunogenic form. to further explore the potential of chloroplasts for the production of bacterial lipoproteins ... | 2007 | 17922845 |
| separate base usages of genes located on the leading and lagging strands in chlamydia muridarum revealed by the z curve method. | the nucleotide compositional asymmetry between the leading and lagging strands in bacterial genomes has been the subject of intensive study in the past few years. it is interesting to mention that almost all bacterial genomes exhibit the same kind of base asymmetry. this work aims to investigate the strand biases in chlamydia muridarum genome and show the potential of the z curve method for quantitatively differentiating genes on the leading and lagging strands. | 2007 | 17925038 |
| [eosinophilic fasciitis associated with borrelia burgdorferi infection]. | eosinophilic fasciitis (shulman syndrome) is defined by the association of sclerodermatous skin changes involving underlying fascia and hypereosinophilia. while the aetiology is unknown, some observations suggest an infectious origin. we report the association of eosinophilic fasciitis with an infection involving borrelia burgdorferi. | 2007 | 17925693 |
| clinicopathological features of primary cutaneous b-cell lymphomas from an academic regional hospital in central italy: no evidence of borrelia burgdorferi association. | we reviewed the clinico-pathological features of 73 primary cutaneous b-cell lymphomas (pcbcls), diagnosed in 10 years in marche region in central italy, which included 16 marginal zone lymphomas (mzl), 33 follicle centre lymphomas (fcl) and 24 diffuse large b cell lymphomas (dlbcl). we also investigated the presence of borrelia burgdorferi in tissues by polymerase chain reaction. differences in age, sex, location site, response to therapy, disease recurrence and 5-year disease-specific survival ... | 2007 | 17926178 |
| [a late revealing of a case of tick-born borreliosis]. | nowadays, many researchers believe that there is a firm ethiopathogenetic association between limited focal scleroderma and focal dermal atrophy, on the one part, and lyme borreliosis, on the other. reports on a possible role of borrelia bugdorferi in the ethiology of scleroderma, which appeared in press in the 90s of the last century, made it possible to confirm a case of tick-born borreliosis (tbb) described below after clinical and specific laboratory examination (antibody immunofluorescence ... | 2007 | 17926497 |
| phagocytosis of borrelia burgdorferi, the lyme disease spirochete, potentiates innate immune activation and induces apoptosis in human monocytes. | we have previously demonstrated that phagocytosed borrelia burgdorferi induces activation programs in human peripheral blood mononuclear cells that differ qualitatively and quantitatively from those evoked by equivalent lipoprotein-rich lysates. here we report that ingested b. burgdorferi induces significantly greater transcription of proinflammatory cytokine genes than do lysates and that live b. burgdorferi, but not b. burgdorferi lysate, is avidly internalized by monocytes, where the bacteria ... | 2008 | 17938216 |
| morphoea: a manifestation of infection with borrelia species? | morphoea or localized scleroderma is a cutaneous inflammatory disease with still unknown aetiology. borrelia burgdorferi as causative agent has been discussed controversially. | 2007 | 17941947 |
| temporomandibular joint involvement caused by borrelia burgdorferi. | lyme borreliosis is an endemic disease in slovenia with an incidence of around 150 patients per 100,000 inhabitants. although the large joints are most typically affected in lyme borreliosis, there are also periods of disease activity with arthritis or arthralgias involving smaller joints, including the temporo-mandibular joint. | 2007 | 17942315 |
| detection and identification of borrelia burgdorferi sensu lato genospecies in ticks from three different regions in slovakia. | lyme borreliosis is one of the most common tick-borne diseases that occur in slovakia. in this study, borrelia burgdorferi sensu lato was detected and cultivated from questing ticks collected in three areas of slovakia. two methods, restriction fragment length polymorphism and reverse line blot, were used for identification of isolates and determination of the prevalence of b. burgdorferi s.l. in the ticks. the prevalence of b. burgdorferi s.l. in i. ricinus detected by reverse line blot was 31. ... | 2007 | 17943405 |
| decline in the frequencies of borrelia burgdorferi ospa161 175-specific t cells after antibiotic therapy in hla-drb1*0401-positive patients with antibiotic-responsive or antibiotic-refractory lyme arthritis. | synovitis in patients with antibiotic-refractory lyme arthritis persists for months to several years after antibiotic therapy. this course, which may result from infection-induced autoimmunity, is associated with t cell recognition of borrelia burgdorferi outer surface protein a (ospa(161-175)) and with hla-dr molecules that bind this epitope, including the drb1*0401 molecule. in this study, we used tetramer reagents to determine the frequencies of ospa(161-175)-specific t cells in samples of pb ... | 2007 | 17947711 |
| acute respiratory distress syndrome in persons with tickborne relapsing fever--three states, 2004-2005. | tickborne relapsing fever (tbrf) is a bacterial illness caused by certain species of borrelia and transmitted through brief and painless bites from ornithodoros ticks. illness usually is characterized by intermittent periods of fever, fatigue, and muscle aches. in april 2005, cdc received reports of two cases of severe tbrf associated with acute respiratory distress syndrome (ards) in residents of california and nevada. after a report describing these cases was posted on cdc's epidemic informati ... | 2007 | 17947965 |
| [epidemiology of borreliosis in workers of the district forestry offices in lower silesia]. | the work presents epidemiological analysis of borrelia burgdorferi (b.b.) infections and borreliosis cases in workers of the district forestry offices in lower silesia in 2003-2005. the infections rate was proved to be high and increasing in time. in 2003-2005 the presence of b.b. antibodies was noted in 672 (35%) examined people. in particular district forestry offices the rate of positive results was between 19.42-50.50%. in several district forestry offices a marked increase of b.b. infection ... | 2007 | 17956058 |
| a novel leptospiral protein increases icam-1 and e-selectin expression in human umbilical vein endothelial cells. | it has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes borrelia sp. and treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. to investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene lic10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, duf1565. the lic10365 gene was cloned and the protein expressed in ... | 2007 | 17956423 |
| evaluation of the presence of selected viral and bacterial nucleic acids in pericardial samples from dogs with or without idiopathic pericardial effusion. | many viruses have been identified in pericardial fluid and in tissue samples from humans with pericarditis by means of molecular diagnostics. in canine idiopathic pericardial effusion there is as yet no conclusive evidence to support the involvement of an infectious agent. this study was designed to investigate a possible relationship between idiopathic pericardial effusion in dogs and viruses most commonly encountered in humans affected with viral pericarditis. coxsackievirus b3 rna, influenza ... | 2009 | 17959400 |
| infectious disease associations in advanced stage, indolent lymphoma (follicular and nonfollicular): developing a lymphoma prevention strategy. | eradication of helicobacter pylori in gastric mucosa-associated lymphoid tumor can result in lymphoma remission. we prospectively identified/treated infections in nonbulky, advanced stage indolent lymphoma (follicular; nonfollicular lymphoma) eligible for observation. materials and methods: stool h. pylori, hepatitis c and borrelia serologies, borrelia and chlamydia fixed tissue pcr, chlamydia peripheral blood mononuclear cell pcr and hydrogen breath test for small bowel bacterial overgrowth (sb ... | 2008 | 17965114 |
| zinc is the metal cofactor of borrelia burgdorferi peptide deformylase. | peptide deformylase (pdf, e.c. 3.5.1.88) catalyzes the removal of n-terminal formyl groups from nascent ribosome-synthesized polypeptides. pdf contains a catalytically essential divalent metal ion, which is tetrahedrally coordinated by three protein ligands (his, his, and cys) and a water molecule. previous studies revealed that the metal cofactor is a fe2+ ion in escherichia coli and many other bacterial pdfs. in this work, we found that pdfs from two iron-deficient bacteria, borrelia burgdorfe ... | 2007 | 17977509 |
| [lyme carditis--a bitter lesson or a delayed diagnostic success--a case report]. | lyme carditis is a well known disorder; however, its diagnosis still remains a challenge because of varied clinical picture, low incidence rate and difficulties in detection of the aetiological agent (borrelia burgdorferi). we report a case of a 60-year-old man with a 2.5-year history of dilated cardiomyopathy, recurring episodes of acute heart failure and arrhythmias which finally were diagnosed as lyme carditis. the diagnosis was confirmed by endomyocardial biopsy that revealed spirochetes as ... | 2007 | 17979052 |
| lyme borreliosis incidence in two french departments: correlation with infection of ixodes ricinus ticks by borrelia burgdorferi sensu lato. | we conducted a prospective study to estimate the lyme borreliosis incidence in two rural french departments, meuse and puy-de-dôme. concurrently, we investigated the prevalence of ticks infected with borrelia burgdorferi sensu lato (sl) and anaplasma phagocytophilum. the incidence of lyme borreliosis decreased from 156 to 109/100,000 inhabitants in meuse and from 117 to 76/100,000 inhabitants in puy-de-dôme in 2004 and 2005, respectively, corresponding to a decrease in the density of ixodes rici ... | 2007 | 17979533 |
| a hard tick relapsing fever group spirochete in a brazilian rhipicephalus (boophilus) microplus. | tick-borne diseases usually comprise a complex epidemiological and ecological network connecting the vector, pathogen, and a group of host species. symptoms associated with lyme disease have been reported in brazil, but no borrelia sp. has been definitively related to these events. here we have identified a b. lonestari/b. theileri-related spirochete dna in the cattle tick rhipicephalus (boophilus) microplus from brazil. four hundred r. microplus and 80 amblyomma cajennense ticks were screened, ... | 2007 | 17979536 |
| a comparison of two dna extraction approaches in the detection of borrelia burgdorferi sensu lato from live ixodes ricinus ticks by pcr and reverse line blotting. | we tested two approaches to extract borrelia dna from live ixodes ricinus ticks before polymerase chain reaction (pcr) and reverse line blotting (rlb): dna extraction of one half of the tick after incubation in bsk medium and dna extraction of the other half of the tick directly, using ammonium hydroxide. among 2079 ticks, 31.2% (n=649) were found to be borrelia-infected by pcr-rlb test using at least one of the dna extraction methods. five hundred four ticks (24.2%) were found infected after in ... | 2007 | 17979538 |
| ticks and associated pathogens collected from domestic animals in the netherlands. | following an outbreak of autochthonous canine babesiosis in the netherlands, a request made to veterinarians and the public to collect ticks from companion animals resulted in 4298 ticks submitted between july 2005 and october 2006 to our center. ticks were identified as ixodes ricinus adults (2907/4298, 67.6%), ixodes sp. nymphs (529/4298, 12.3%) and ixodes sp. larvae (385/4298, 9.0%), i. hexagonus adults (328/4298, 7.6%), dermacentor reticulatus (72/4298, 1.7%), and several other exotic tick s ... | 2007 | 17979540 |
| bradycardic child: what's to blame? | | 2007 | 17980807 |
| the association between tick-borne infections, lyme borreliosis and autism spectrum disorders. | chronic infectious diseases, including tick-borne infections such as borrelia burgdorferi may have direct effects, promote other infections and create a weakened, sensitized and immunologically vulnerable state during fetal development and infancy leading to increased vulnerability for developing autism spectrum disorders. a dysfunctional synergism with other predisposing and contributing factors may contribute to autism spectrum disorders by provoking innate and adaptive immune reactions to cau ... | 2008 | 17980971 |
| fitness variation of borrelia burgdorferi sensu stricto strains in mice. | lyme borreliosis in north america is caused by the tick-borne spirochete borrelia burgdorferi, a zoonotic bacterium that is able to persistently infect a wide range of vertebrate species. given the pronounced strain structure of b. burgdorferi in the northeastern united states, we asked whether the fitness of the different genotypes varies among susceptible vertebrate hosts. the transmission dynamics of two genetically divergent human isolates of b. burgdorferi, bl206 and b348, were analyzed exp ... | 2008 | 17981941 |
| role of the bba64 locus of borrelia burgdorferi in early stages of infectivity in a murine model of lyme disease. | borrelia burgdorferi, the causative agent of lyme disease, undergoes rapid adaptive gene expression in response to environmental signals encountered during different stages of its life cycle in the arthropod vector or the mammalian host. among all the plasmid-encoded genes of b. burgdorferi, several linear plasmid 54 (lp54)-encoded open reading frames (orfs) exhibit the greatest differential expression in response to mammalian host-specific temperature, ph, and other uncharacterized signals. the ... | 2008 | 17984202 |
| global transcriptome analysis of borrelia burgdorferi during association with human neuroglial cells. | as adherence and entry of a pathogen into a host cell are key components to an infection, identifying the molecular mechanisms responsible for cellular association will provide a better understanding of a microbe's pathogenesis. we previously established an in vitro model for borrelia burgdorferi infection of human neuroglial cells. to expand on our earlier study, we performed b. burgdorferi whole-genome expression analysis following a 20-hour infection of human neuroglial cells to identify borr ... | 2008 | 17984208 |
| beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing. | we investigated how the register between adjacent beta-strands is specified using a series of mutants of the single-layer beta-sheet (slb) in borrelia ospa. the single-layer architecture of this system eliminates structural restraints imposed by a hydrophobic core, enabling us to address this question. a critical turn (turn 9/10) in the slb was replaced with a segment with an intentional structural mismatch. its crystal structure revealed a one-residue insertion into the central beta-strand (str ... | 2007 | 17985889 |
| [inflammatory cardiac diseases by primary extracardial diseases]. | as systemic immunological disorders, internal diseases in gastroenterology, rheumatology and infectiology can, in addition to the bowels, potentially involve the musculo-skeletal system, the immunological system and heart structures. all structures and functions of the heart can be affected. pericarditis in lupus erythematosus and chronic inflammatory bowel disease, myocarditis in hiv infection and lyme disease are examples of cardiac manifestations of internal diseases. the pathogenetic causes ... | 2008 | 17992497 |
| spatial analysis of the exposure of dogs in rural north-coastal california to vectorborne pathogens. | between 0 and 50 per cent of the dogs in eight rural villages in far northern california with a high risk of tickborne diseases were seropositive for anaplasma phagocytophilum and bartonella vinsonii subspecies berkhoffii, and between 0 and 10 per cent were seropositive for borrelia burgdorferi. the odds ratio for the co-exposure of individual dogs to b vinsonii berkhoffii and a phagocytophilum was 18.2. none of the diseases was associated with the sex of the dogs, whether they slept out of door ... | 2007 | 17993656 |
| gencolors: annotation and comparative genomics of prokaryotes made easy. | gencolors (gencolors.fli-leibniz.de) is a new web-based software/database system aimed at an improved and accelerated annotation of prokaryotic genomes considering information on related genomes and making extensive use of genome comparison. it offers a seamless integration of data from ongoing sequencing projects and annotated genomic sequences obtained from genbank. a variety of export/import filters manages an effective data flow from sequence assembly and manipulation programs (e.g., gap4) t ... | 2007 | 17993668 |
| lyme arthritis: current concepts and a change in paradigm. | | 2008 | 18003815 |
| quantitative measurement of c6 antibody following antibiotic treatment of borrelia burgdorferi antibody-positive nonclinical dogs. | the detection of antibody to the borrelia burgdorferi c6 peptide by use of enzyme-linked immunoassays is a widely accepted method for the diagnosis of lyme disease spirochete infection in dogs and in humans. antibody to the c6 peptide is highly specific for b. burgdorferi and declines following treatment of dogs and humans exposed to b. burgdorferi. a quantitative assay for determining c6 antibody levels was developed and used to measure changes in antibody levels following antibiotic treatment ... | 2008 | 18003819 |
| spit-acular entry: borrelia gets help from a tick salivary protein to move from the mammalian host to the arthropod vector. | in vector-host-pathogen interactions, vector saliva plays a key role in the successful acquisition of blood and in facilitating the establishment of pathogens in mammalian hosts. in this issue of cell host & microbe, narasimhan et al. describe how a tick salivary antioxidant neutralizes reactive oxygen species at the tick-host interface and facilitates the tick vector's acquisition of borrelia burgdorferi bacteria from an infected mammalian host. | 2007 | 18005711 |
| a tick antioxidant facilitates the lyme disease agent's successful migration from the mammalian host to the arthropod vector. | the tick ixodes scapularis is an efficient vector for microbes, including the lyme disease agent borrelia burgdorferi. ticks engorging on vertebrates induce recruitment of inflammatory cells to the bite site. for efficient transmission to the vector, pathogens have to traffic through this complex feeding site while avoiding the deleterious effects of immune cells. we show that a tick protein, salp25d, plays a critical role-in the mammalian host-for acquisition of borrelia burgdorferi by the vect ... | 2007 | 18005713 |
| effect of proteins from the spirochete borrelia burgdorferi sensu lato on myelinated nerve excitability. | we studied the effect of spirochete borrelia burgdorferi sensu lato cell membrane proteins on excitability of myelinated nerve fiber. it was found that cell surface proteins of spirochetes b. burgdorferi s. s. bind to ranvier nodes of the axon and to schwann cells. binding of b. burgdorferi s. s. and b. garinii to the nerve fiber modulates the amplitude and conduction velocity of the action potential, while b. afzelii had no effect on these parameters. the decrease in the spike amplitude and con ... | 2007 | 18019007 |
| does tick-borne relapsing fever have an animal reservoir in east africa? | tick-borne relapsing fevers (tbrf) are caused by infection with borrelia spirochetes and transmitted to humans by ticks. all except east african tbrf, caused by borrelia duttonii, are known zoonoses. this widespread, endemic and pathogenic infection has only been found in humans and the ornithodoros sp. soft tick vectors. we investigated the role of domestic animals as possible reservoirs of infection in a tbrf endemic region. tick infestations in households and pigpens were investigated in the ... | 2007 | 18021022 |
| surveillance for zoonotic vector-borne infections using sick dogs from southeastern brazil. | for many vector-borne organisms, dogs can be used as sentinels to estimate the risk of human infection. the objective of this study was to use dogs as sentinels for multiple vector-borne organisms in order to evaluate the potential for human infection with these agents in southeastern brazil. blood from 198 sick dogs with clinicopathological abnormalities consistent with tick-borne infections were selected at the são paulo state university veterinary teaching hospital in botucatu and tested for ... | 2007 | 18021025 |
| borrelia burgdorferi sensu lato in siberian chipmunks (tamias sibiricus) introduced in suburban forests in france. | numerous vertebrate reservoirs have been described for borrelia burgdorferi sensu lato (sl), which includes the etiological agents of lyme borreliosis (lb). the siberian chipmunk (tamias sibiricus) is a rodent originating from asia, where it is suspected to be a b. burgdorferi reservoir. it has been intentionally released into the wild in europe since the 1970s, but has not yet been subject to any study regarding its association with the lb agent. in this paper we studied siberian chipmunk infes ... | 2007 | 18021026 |
| detection of spirochetal micro-organisms by focus-floating microscopy in necrobiotic xanthogranuloma. | necrobiotic xanthogranuloma (nxg) is a rare histiocytic disorder of unknown origin. | 2007 | 18021850 |
| borrelia burgdorferi--a potentially aetiological factor in tmj disorders? preliminary report. | the available literature does not report on infectious factors potentially triggering intra-articular pathology or dysfunction manifest as temporomandibular (tmj) disorders. | 2008 | 18023198 |
| retrobulbar optic neuritis: a complication of lyme disease? | | 2007 | 18024698 |
| opsoclonus myoclonus syndrome in two cases with neuroborreliosis. | | 2007 | 18028183 |
| conspicuous impacts of inconspicuous hosts on the lyme disease epidemic. | emerging zoonotic pathogens are a constant threat to human health throughout the world. control strategies to protect public health regularly fail, due in part to the tendency to focus on a single host species assumed to be the primary reservoir for a pathogen. here, we present evidence that a diverse set of species can play an important role in determining disease risk to humans using lyme disease as a model. host-targeted public health strategies to control the lyme disease epidemic in north a ... | 2008 | 18029304 |
| fully automated, homogeneous nucleic acid detection technology based on dry-reagent assay chemistry and time-resolved fluorometry. | | 2007 | 18030698 |
| [specifics of cytokine regulation in tick-borne encephalitis and lyme borreliosis]. | the aim of the study was to evaluate the dynamics of t-lymphocyte cytokine profile during the acute period of tick-bore encephalitis (febrile and meningeal forms) and non-erythematous lyme borreliosis (nelb). elisa (vector-best, novosybirsk) and iha (virion, tomsk) techniques were used for laboratory diagnostics of tick-borne encephalitis (tbe). etiological verification of lyme borreliosis (lb) was performed using immune-enzyme test systems for detection of igg and igm antibodies to borellia bur ... | 2007 | 18030714 |
| cutaneous b-cell pseudolymphoma at the site of vaccination. | pseudolymphomas are a rare complication of vaccination, presenting with dense lymphoid infiltrates and prominent follicular pattern. we report our observations on 4 patients with vaccination-induced b-cell pseudolymphoma (all females; age range 19 to 60 years; median: 34.5 years). clinically 3 patients presented with subcutaneous nodules and 1 presented with a large, indurated, erythematous plaque. histology revealed in all cases dense lymphoid infiltrates in the subcutaneous fat with prominent ... | 2007 | 18032948 |
| [acute myelitis and lyme disease]. | acute myelitis accounts for 4 to 5 percent of all cases of neuroborreliosis. in the literature, simultaneous spinal mri and cerebrospinal fluid (csf) investigations are presented for only 8 cases. we describe here 3 cases of acute lyme myelitis. | 2007 | 18033042 |
| malt lymphoma : recent advances in aetiology and molecular genetics. | mucosa-associated lymphoid tissue (malt) lymphoma is a common low grade b-cell lymphoma arising from a background of chronic inflammatory disease at a number of mucosal sites. those originating in the stomach are causatively linked to helicobacter pylori infection and eradication of the bacterium with antibiotics leads to long-term complete regression of the lymphoma in aproximately 70% of cases. now, there is further evidence of linking campylobacter jejuni, borrelia burgdorferi and chlamydia p ... | 2007 | 18040143 |
| [comparison of test with antigen vlse (c6) with tests with recombinant antigens in patients with lyme borreliosis]. | in diagnosis of lyme borreliosis classic, recombinant antigens are used. introduced recombinant antigen vlse increases hope to improve sensibility of the tests. | 2007 | 18044336 |
| [clinical forms of neuroborreliosis among hospitalized patients in the years 2000-2005]. | to evaluate the frequency of clinical forms as well as laboratory and neuroimaging results of patients with diagnosed neuroborreliosis in the years 2000-2005 due to neuroborreliosis. | 2007 | 18044338 |
| [immunopathology of lyme arthritis]. | lyme borreliosis (lyme disease) is the most prevalent tick-borne disease caused by spirochaetes of the borrelia species complex. arthritis is one of the common manifestations of b. burgdorferi infection. the pathomechanism of articular changes in lyme arthritis has not yet been elucidated. histopathological studies of synovia and immunological changes are similar to rheumatoid arthritis. in the early stage of inflammation b. burgdorferi interact with polynuclear granulocytes and epithelial cells ... | 2007 | 18044347 |
| third degree atrioventricular block and accelerated idioventricular rhythm associated with a heart base chemodectoma in a syncopal rottweiler. | a 7-year-old male intact rottweiler was presented with a 1-week history of lethargy, anorexia, vomiting and multiple syncopal events. the results of the clinical examination and electrocardiography were consistent with a third degree atrioventricular block and an intermittent accelerated idioventricular rhythm. haematology, serum biochemistry, serology for borrelia burgdorferi, blood culture, total t4, thoracic radiography and echocardiography did not reveal the cause of the arrhythmia. response ... | 2007 | 18045349 |
| identification of host bloodmeal source and borrelia burgdorferi sensu lato in field-collected ixodes ricinus ticks in chaumont (switzerland). | to evaluate the importance of vertebrate species as tick hosts and as reservoir hosts in two endemic areas for lyme borreliosis in switzerland, we applied molecular methods for the analysis of bloodmeal source and borrelia infection in questing ixodes ricinus l. ticks. in total, 1326 questing ticks were simultaneously analyzed for borrelia and for blood meal remnants by using reverse line blot. an overall infection prevalence of 19.0% was recorded for borrelia sp., with similar rates in both sit ... | 2007 | 18047213 |
| optic neuropathy from viruses and spirochetes. | | 2007 | 18049280 |
| [borreliosis as the cause of disability pensions in norway]. | borreliosis is a bacterial infection transferred by tick-bites. neuroborreliosis is the most frequent disseminated form of the disorder in norway. registers exist in norway on all reported communicable diseases (the norwegian surveillance system for communicable diseases [msis]) and disability pension diagnoses (the norwegian directorate of labour and welfare). | 2007 | 18049495 |
| antibody responses to borrelia burgdorferi in patients with antibiotic-refractory, antibiotic-responsive, or non-antibiotic-treated lyme arthritis. | to compare the pattern of antibody responses to borrelia burgdorferi in patients with antibiotic-refractory, antibiotic-responsive, or non-antibiotic-treated lyme arthritis as an indirect measure of spirochetal persistence or eradication. | 2007 | 18050219 |
| detection of rickettsia amblyommii in association with a tick bite rash. | in the summer of 2006, an amblyomma americanum tick was removed from a woman in central north carolina, who subsequently developed a rash at the site of tick attachment. when examined by polymerase chain reaction (pcr) for borrelia, anaplasma, ehrlichia, babesia, rickettsia, and bartonella dna, only the rickettsia primers generated an amplicon, which was identified as "r. amblyommii" by sequencing. to our knowledge, this is the first case in which r. amblyommii was temporally associated with a r ... | 2007 | 18052716 |
| cd28 deficiency exacerbates joint inflammation upon borrelia burgdorferi infection, resulting in the development of chronic lyme arthritis. | lyme disease, caused by the tick-borne spirochete borrelia burgdorferi (bb), is a multisystem illness, affecting many organs, such as the heart, the nervous system, and the joints. months after bb infection, approximately 60% of patients experience intermittent arthritic attacks, a condition that in some individuals progresses to chronic joint inflammation. although mice develop acute arthritis in response to bb infection, the joint inflammation clears after 2 wk, despite continuous infection, o ... | 2007 | 18056348 |
| immune complexes in early lyme disease. | the study investigated the presence of borrelia-specific antibodies captured in immune complexes (ics) in patients with early lyme disease manifested by erythema migrans. out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ics containing igm antibodies, while only 4 (22.2%) were igg positive. these results are in accordance with our findings obtained by standard elisa and recombinant blot, which indicated that ics might be used for serological diagnosis of the ear ... | 2007 | 18059570 |
| structural insights into the enzymatic mechanism of the pathogenic mapk phosphothreonine lyase. | the ospf family of phosphothreonine lyase, including spvc from salmonella, irreversibly inactivates the dual-phosphorylated host mapks (pt-x-py) through beta elimination. we determined crystal structures of spvc and its complex with a phosphopeptide substrate. spvc adopts a unique fold of alpha/beta type. the disordered n terminus harbors a canonical d motif for mapk substrate docking. the enzyme-substrate complex structure indicates that recognition of the phosphotyrosine followed by insertion ... | 2007 | 18060821 |
| phenotypic and genotypic analysis of borrelia spp. isolated from ixodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction. | the genotype of borrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks. borrelia determination was based on partial sequencing of the 16s rrna gene and real-time polymerase chain reactions for identification and quantitation of ospa and reca genes. different borrelia spp. were identified; b. garinii in 40% ticks followed by b. afzelii (36.3%), b. burgdorferi sensu stricto (12.9%), b. valaisiana (3.5%), b. lusitaniae (0.8%), b. bissettii (0.5%) and b. miyamotoi-like (0.5%). cultiva ... | 2007 | 18062179 |
| [what kind of clinical, epidemiological, and biological data is essential for the diagnosis of lyme borreliosis? dermatological and ophtalmological courses of lyme borreliosis]. | lyme borreliosis (bl) is a multisystem infectious tick-transmitted disease. the diversity of borrelia burgdorferi is the reason for a wide spectrum of dermatological and ophthalmologic presentations between patients from europe and from other countries. in europe, the main manifestations are dermatological. during the early stage, the diagnosis is clinical: finding erythema migrans (em) a few days after a tick bite is sufficient; several em mean an early-disseminated disease. borrelial lymphocyt ... | 2007 | 18065181 |
| borrelia burgdorferi inhibits human neutrophil functions. | outer surface proteins ospa and ospb are among the most prominent borrelia burgdorferi surface molecules. we constructed ospab and ospa complementation mutants of b. burgdorferi osp-less strain b313 and investigated the role of these surface proteins in the interactions of b. burgdorferi, human neutrophils and the complement system. we found that (1) ospb inhibits the phagocytosis and oxidative burst of human neutrophils at low serum concentrations, whereas ospa induces the oxidative burst in ne ... | 2008 | 18068388 |
| [the modern therapy of lyme disease]. | the modern therapy of lyme disease, including newest guidelines of the infectious diseases society of america has been presented. poland is endemic region for borreliosis and number of reported cases have been increasing rapidly. newest guidelines should help doctors, not only infectious disease specialist, but especially general practicioners, solve problems assiciated with therapy of lyme disease. | 2007 | 18069380 |
| an insight into the sialome of the soft tick, ornithodorus parkeri. | while hard ticks (ixodidae) take several days to feed on their hosts, soft ticks (argasidae) feed faster, usually taking less than 1h per meal. saliva assists in the feeding process by providing a cocktail of anti-hemostatic, anti-inflammatory and immunomodullatory compounds. saliva of hard ticks has been shown to contain several families of genes each having multiple members, while those of soft ticks are relatively unexplored. analysis of the salivary transcriptome of the soft tick ornithodoru ... | 2008 | 18070662 |
| [significance of red deer (cervus elaphus) in the ecology of borrelia burgdorferi sensu lato]. | background: red deer (cervus elaphus) is one of the most important host of the adult tick (ixodes ricinus) which is the basic vector of the lyme disease causative agent--borrelia burgdorferi sensu lato in europe. the aim of the present study was to establish the role of red deer in the transmission of b. burgdorferi s.1. material and methods. tissues from 74 red deers were evaluated and the presence of b. burgdorferi s.1 dna was identified using nested pcr technique based on fla gene. the identi ... | 2007 | 18075156 |
| [frequency of antibodies anti-ehrlichia canis, borrelia burgdorferi and dirofilaria immitis antigens in dogs from microrregion ilhéus-itabuna, state of bahia, brazil]. | in order to detect the positivity to antigens of dirofilaria immitis, antibodies anti-borrelia burgdorferi and anti-ehrlichia canis, 200 canine blood samples were collected as followed: 100 from the municipality of ilhéus and 100 from itabuna, state of bahia. the kit snap 3dx (idexx laboratories) was used to performe serology. none of the tested animalswere positive for antigens of d. immitis. only two dogs of them were positive for antibodies anti-b. burgdorferi. from all the samples analyzed, ... | 2007 | 18078596 |
| the important and diverse roles of antibodies in the host response to borrelia infections. | antibodies are of critical importance in the host response to tick-borne borrelia species that cause relapsing fever and lyme disease. recent studies on the role of various b cell subsets in the host response to borrelia, complement-independent, bactericidal antibodies, and diagnostics led to this review that focuses on the array of functions that antibodies to borrelia can perform. | 2008 | 18080415 |
| evaluation of a genotyping method based on the ospa gene to detect borrelia burgdorferi sensu lato in multiple samples of lyme borreliosis patients. | in this study we have developed a new restriction-fragment-length-polymorphism (rflp) genotyping method for rapid detection and identification of borrelia genospecies present as unique species or as co-infection in multiple specimens obtained simultaneously from 29 individual patients affected by early or late lyme borreliosis (lb). the target of the rflp-genotyping was the heterogeneous plasmid located ospa gene, thus we developed a method able to detect and differentiate between six clinically ... | 2007 | 18080675 |
| [concentration of soluble forms of selectins in serum and in cerebrospinal fluid in group of patients with neuroborreliosis--a preliminary study]. | the results of the research already done, suggest an important role of selectins in inflammatory process of various etiology. lack of selectins or their ligands causes severe complications, such as chronic inflammatory processes. the aim of this study was to analyze the role of selectins sl, se and sp in the development and course of neuroborreliosis in the form of meningitis. we have also analyzed the influence of treatment on changes of selectins' concentration in serum and cerebrospinal fluid ... | 2007 | 18080689 |
| t cell infiltration is associated with increased lyme arthritis in tlr2-/- mice. | c57bl/6 mice deficient in tlr2 develop more severe arthritis following infection with borrelia burgdorferi than do wild-type c57bl/6 mice, and this increase is suppressed by the simultaneous presence of the scid mutation. this suggested a requirement for lymphocytes in the development of subacute lyme arthritis in tlr2(-/-) mice, a feature not commonly associated with this arthritis. the increased pathology of b. burgdorferi-infected tlr2(-/-) mice was also accompanied by an increase in mononucl ... | 2008 | 18081848 |