| comparative genomics and metabolomics analyses of the adaptation mechanism in ketogulonicigenium vulgare-bacillus thuringiensis consortium. | adaptive evolution by serial subcultivation of co-cultured bacillus thuringiensis and ketogulonicigenium vulgare significantly enhanced the productivity of 2-keto-l-gulonic acid in two-step vitamin c production. the adaptation mechanism in k. vulgare-b. thuringiensis consortium was investigated in this study based on comparative genomics and metabolomics studies. it was found that the growth, anti-oxidation, transcription and regulation were significantly enhanced in the adapted consortium. the ... | 2017 | 28440340 |
| horizontal transfer of chromosomal markers mediated by the large conjugative plasmid pxo16 from bacillus thuringiensis serovar israelensis. | pxo16, a large plasmid originating from bacillus thuringiensis serovar israelensis, displays unique conjugation capacities: besides efficient self-transfer, it is able to mobilize and retro-mobilize non-conjugative plasmids, including those missing an orit and/or a mob gene, also known as "non-mobilizable" plasmids. in this paper, another peculiar transfer property of pxo16 is described. this element is indeed able to transfer chromosomal loci at frequencies of ca. 10(-5)-10(-6) transconjugants/ ... | 2017 | 28435006 |
| field trial performance of herculex xtra (cry34ab1/cry35ab1) and smartstax (cry34ab1/cry35ab1 + cry3bb1) hybrids and soil insecticides against western and northern corn rootworms (coleoptera: chrysomelidae). | western corn rootworm, diabrotica virgifera virgifera leconte (coleoptera: chrysomelidae), and northern corn rootworm, diabrotica barberi smith and lawrence (coleoptera: chrysomelidae), are important insect pests in corn, zea mays l. for more than a decade, growers have been using transgenic plants expressing proteins from the bacterium bacillus thuringiensis (bt) to protect corn roots from feeding. in 2011, western corn rootworm populations were reported to have developed resistance to bt hybri ... | 2017 | 28430986 |
| probing the germination kinetics of ethanol-treated bacillus thuringiensis spores. | bacillus thuringiensis (bt) is the most widely used microbial insecticide. to clarify the mechanism of bacterial resistance to ethanol toxicity, the present study investigated the effects of 70% (v/v) ethanol at a moderate temperature (65°c) on bt spore germination by single-cell raman spectroscopy and differential interference contrast microscopy. we found that over 80% of bt spores were inviable after 30 min of treatment. moreover, ethanol treatment affected spore germination; the time for ini ... | 2017 | 28430241 |
| personality, immune response and reproductive success: an appraisal of the pace-of-life syndrome hypothesis. | the pace-of-life syndrome (pols) hypothesis is an extended concept of the life-history theory that includes behavioural traits. the studies challenging the pols hypothesis often focus on the relationships between a single personality trait and a physiological and/or life-history trait. while pathogens represent a major selective pressure, few studies have been interested in testing relationships between behavioural syndrome, and several fitness components including immunity. the aim of this stud ... | 2017 | 28425582 |
| cry toxin specificities of insect abcc transporters closely related to lepidopteran abcc2 transporters. | in this study, we examined insect and human abcc transporters closely related to the lepidopteran abc transporter c2 (abcc2), a powerful receptor for the bacillus thuringiensis cry toxin, for their responses to various cry toxins. abcc2 and the lepidopteran abc transporter c3 (abcc3) conferred cultured cells with susceptibility to a lepidopteran-specific cry1aa toxin but not to lepidopteran-specific cry1ca and cry1da. one coleopteran abcc transporter specifically responded to a coleopteran-speci ... | 2017 | 28416297 |
| antibacterial activity of novel peptide derived from cry1ab16 toxin and development of lbl films for foodborne pathogens control. | escherichia coli is one of the most common etiological agents of diarrhea in developing countries. the appearance of resistant e. coli prevents treatment of these infections. biotechnological products incorporating antimicrobial peptides are currently being considered in applications to prevent intestinal infections by these bacteria. the aim of this study was to evaluate the antibacterial activity of the peptide pcl342-354c, which is derived from the toxin cry1ab16 from bacillus thuringiensis, ... | 2017 | 28415492 |
| assessment of the antimicrobial activity and the entomocidal potential of bacillus thuringiensis isolates from algeria. | this work represents the first initiative to analyze the distribution of b. thuringiensis in algeria and to evaluate the biological potential of the isolates. a total of 157 isolates were recovered, with at least one isolate in 94.4% of the samples. the highest bt index was found in samples from rhizospheric soil (0.48) and from the mediterranean area (0.44). most isolates showed antifungal activity (98.5%), in contrast to the few that had antibacterial activity (29.9%). a high genetic diversity ... | 2017 | 28406460 |
| a new biopesticide from a local bacillus thuringiensis var. tenebrionis (xd3) against alder leaf beetle (coleoptera: chrysomelidae). | use of chemical pesticides in agriculture harms humans, non-target organisms and environments, and causes increase resistance against chemicals. in order to develop an effective bio-pesticide against coleopterans, particularly against agelastica alni (coleoptera: chrysomelidae) which is one of the serious pests of alder leaf and hazelnut, we tested the insecticidal effect of 21 bacillus isolates against the larvae and adults of the pest. bacillus thuringiensis var. tenebrionis-xd3 (btt-xd3) show ... | 2017 | 28405911 |
| comparative analysis of the genetic basis of cry1f resistance in two strains of spodoptera frugiperda originated from puerto rico and florida. | the fall armyworm, spodoptera frugiperda (j.e. smith), is a major target pest of bacillus thuringiensis (bt) maize and cotton in america. since the commercialization of cry1f maize (event tc1507) in 2003, resistance to cry1f maize in field populations of s. frugiperda has occurred in puerto rico, brazil and the southeast region of the united states. in this paper, we conducted a comparative analysis of the inheritance of two cry1f-resistant colonies of s. frugiperda originated from puerto rico ( ... | 2017 | 28392284 |
| molecular characterization and pcr-based screening of cry genes from bacillus thuringiensis strains. | novel cry genes are potential candidates for resistance management strategies, due to their different structures and modes of action. therefore, it is desirable to clone and express novel cry genes from several new isolates of bacillus thuringiensis (bt). in the present study, 28 bt strains were characterized at morphological and molecular level. all these strains are gram positive, endospore forming and had shown different crystal morphologies when viewed under the microscope. the ardra (16s rd ... | 2017 | 28391468 |
| holotrichia oblita midgut proteins that bind to bacillus thuringiensis cry8-like toxin and assembly of the h. oblita midgut tissue transcriptome. | the bacillus thuringiensis strain hbf-18 (cgmcc 2070), containing two cry genes (cry8-like and cry8ga), is toxic to holotrichia oblita larvae. both cry8-like and cry8ga proteins are active against this insect pest, and cry8-like is more toxic. to analyze the characteristics of the binding of cry8-like and cry8ga proteins to brush border membrane vesicles (bbmvs) in h. oblita larvae, binding assays were conducted with a fluorescent dylight488-labeled cry8-like toxin. the results of saturation bin ... | 2017 | 28389549 |
| emira: ecologic malaria reduction for africa - innovative tools for integrated malaria control. | background malaria control is based on early treatment of cases and on vector control. the current measures for malaria vector control in africa are mainly based on long-lasting insecticide treated nets (llins) and to a much smaller extent on indoor residual spraying (irs). a third pillar in the fight against the malaria vector, larval source management (lsm), has virtually not been used in africa since the ban of ddt in the 1960s. within the light of recent who recommendations for bacillus thur ... | 2014 | 28672461 |
| a mathematical model for predicting the persistence and efficacy of bacillus thuringiensis var israelensis in relation to water pollution. | in a simulated field trial, the bead formulation of bacillus thuringiensis var israelensis (bti) was found to be more persistent in moderately polluted as compared to highly polluted water. a mathematical model was built up to study the influence of time (independent variable) on larval reduction (dependent variable) in relation to degree of water pollution. two predictive models for different grades of water pollution were developed, which estimated that the larval mortality in moderately pollu ... | 1998 | 28775440 |
| field trial of bacillus thuringiensis var israelensis pellet formulation in the control of mosquitoes. | in a simulated field trial bacillus thuringiensis var israelensis (bti) pellet formulation exhibited an enhanced efficacy with increasing dose. a dosage of 1.0 and 1.5 ppm was most effective under simulated field conditions. in field trials persistence of bti pellet (1.0 ppm) was observed for 35 days in moderately polluted water collection as compared to 21 days in highly polluted water bodies. | 1996 | 28769402 |
| fitness costs of resistance to bacillus thuringiensis in the diamondback moth (plutella xylostella). | | 1994 | 28567779 |