| [investigation of norovirus infection incidence among 0-5 years old children with acute gastroenteritis admitted to two different hospitals in ankara, turkey]. | norovirus causes severe gastroenteritis requiring hospitalization especially in children less than five years of age both in developed and developing countries. therefore, we aimed to investigate the incidence of norovirus (nov) in 0-5 years old children with acute gastroenteritis in two large hospitals in ankara, turkey. stool samples were obtained from 1000 (413 female, 587 male) children between 0-5 years old with acute gastroenteritis who attended to the department of paediatrics, ministry o ... | 2013 | 23390907 |
| the α-helical regions of kerp1 are important in entamoeba histolytica adherence to human cells. | the lysine and glutamic acid rich protein kerp1 is a unique surface adhesion factor associated with virulence in the human pathogen entamoeba histolytica. both the function and structure of this protein remain unknown to this date. here, we used circular dichroism, analytical ultracentrifugation and bioinformatics modeling to characterize the structure of kerp1. our findings revealed that it is an α-helical rich protein organized as a trimer, endowed with a very high thermal stability (tm = 89.6 ... | 2013 | 23378906 |
| evaluation of formalin-ether sedimentation and trichrome staining techniques: its effectiveness in detecting entamoeba histolytica/dispar/moshkovskii in stool samples. | this study was conducted to evaluate two routinely microscopic diagnostic methods in comparison with single-round pcr assay as the reference technique to detect entamoeba histolytica/dispar/moshkovskii. examination was performed on 500 stool samples obtained from orang asli communities in different states of malaysia using formalin-ether sedimentation, trichrome staining and single-round pcr techniques. ninety-three stool samples were detected e. histolytica/dispar/moshkovskii positive by routin ... | 2013 | 23361047 |
| entamoeba histolytica exacerbates epithelial tight junction permeability and proinflammatory responses in muc2(-/-) mice. | human mucin-2 (muc-2) is the first line of innate host defense in preventing pathogen-induced epithelial injury. entamoeba histolytica (eh) colonizes the mucus layer by binding of the parasite's surface galactose lectin to galactose and n-acetyl-d-galactosamine residues on colonic muc-2, preventing parasite contact-dependent cytolysis of epithelial cells. we quantified early innate responses to eh in wild-type and muc-2-deficient mice (muc2(-/-)) using closed colonic loops. eh infection in wild- ... | 2013 | 23357502 |
| novel tpr-containing subunit of tom complex functions as cytosolic receptor for entamoeba mitosomal transport. | under anaerobic environments, the mitochondria have undergone remarkable reduction and transformation into highly reduced structures, referred as mitochondrion-related organelles (mros), which include mitosomes and hydrogenosomes. in agreement with the concept of reductive evolution, mitosomes of entamoeba histolytica lack most of the components of the tom (translocase of the outer mitochondrial membrane) complex, which is required for the targeting and membrane translocation of preproteins into ... | 2013 | 23350036 |
| small rna pyrosequencing in the protozoan parasite entamoeba histolytica reveals strain-specific small rnas that target virulence genes. | small rna mediated gene silencing is a well-conserved regulatory pathway. in the parasite entamoeba histolytica an endogenous rnai pathway exists, however, the depth and diversity of the small rna population remains unknown. | 2013 | 23347563 |
| catalysis by n-acetyl-d-glucosaminylphosphatidylinositol de-n-acetylase (pig-l) from entamoeba histolytica: new roles for conserved residues. | we showed previously that entamoeba histolytica pig-l exhibits a novel metal-independent albeit metal-stimulated activity. using mutational and biochemical analysis, here we identify asp-46 and his-140 of the enzyme as being important for catalysis. we show that these mutations neither affect the global conformational of the enzyme nor alter its metal binding affinity. the defect in catalysis, due to the mutations, is specifically due to an effect on v(max) and not due to altered substrate affin ... | 2013 | 23341455 |
| label-free electrochemical detection of an entamoeba histolytica antigen using cell-free yeast-scfv probes. | inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scfv) as novel affinity reagents. | 2013 | 23329132 |
| tnfα-mediated liver destruction by kupffer cells and ly6chi monocytes during entamoeba histolytica infection. | amebic liver abscess (ala) is a focal destruction of liver tissue due to infection by the protozoan parasite entamoeba histolytica (e. histolytica). host tissue damage is attributed mainly to parasite pathogenicity factors, but massive early accumulation of mononuclear cells, including neutrophils, inflammatory monocytes and macrophages, at the site of infection raises the question of whether these cells also contribute to tissue damage. using highly selective depletion strategies and cell-speci ... | 2013 | 23300453 |
| prevalence and characters of entamoeba histolytica infection in saudi infants and children admitted with diarrhea at 2 main hospitals at south jeddah: a re-emerging serious infection with unusual presentation. | in this study, entamoeba histolytica had high prevalence and unusual presentation by affecting high proportion of infants under 1 year; severe clinical manifestations, and laboratory findings that were known to be usually encountered in invasive amebiasis as significant leukocytosis for age, neutrophilic leukocytosis for age, and positive c-reactive protein were found among more than 50% of admitted saudi infants and children with e. histolytica infection in our locality. e. histolytica can be a ... | 2013 | 23287546 |
| involvement of a putative intercellular signal-recognizing g protein-coupled receptor in the engulfment of salmonella by the protozoan tetrahymena. | in an effort to investigate the molecular basis of protozoa engulfment-mediated hypervirulence of salmonella in cattle, we evaluated protozoan g protein-coupled receptors (gpcrs) as transducers of salmonella engulfment by the model protozoan tetrahymena. our laboratory previously demonstrated that non-pathogenic protozoa (including tetrahymena) engulf salmonella and then exacerbate its virulence in cattle, but the mechanistic details of the phenomenon are not fully understood. gpcrs were investi ... | 2013 | 26623315 |
| structural determinants of rgs-rhogef signaling critical to entamoeba histolytica pathogenesis. | g protein signaling pathways, as key components of physiologic responsiveness and timing, are frequent targets for pharmacologic intervention. here, we identify an effector for heterotrimeric g protein α subunit (ehgα1) signaling from entamoeba histolytica, the causative agent of amoebic colitis. ehgα1 interacts with this effector and guanosine triphosphatase-accelerating protein, ehrgs-rhogef, in a nucleotide state-selective fashion. coexpression of ehrgs-rhogef with constitutively active ehgα1 ... | 2013 | 23260656 |
| quantification of stochastic noise of splicing and polyadenylation in entamoeba histolytica. | alternative splicing and polyadenylation were observed pervasively in eukaryotic messenger rnas. these alternative isoforms could either be consequences of physiological regulation or stochastic noise of rna processing. to quantify the extent of stochastic noise in splicing and polyadenylation, we analyzed the alternative usage of splicing and polyadenylation sites in entamoeba histolytica using rna-seq. first, we identified a large number of rarely spliced alternative junctions and then showed ... | 2013 | 23258700 |
| regulation of h2o2 stress-responsive genes through a novel transcription factor in the protozoan pathogen entamoeba histolytica. | outcome of infection depends upon complex interactions between the invading pathogen and the host. as part of the host's innate immune response, the release of reactive oxygen and nitrogen species by phagocytes represents a major obstacle to the establishment of infection. the ability of the human parasite entamoeba histolytica to survive reactive oxygen and nitrogen species is central to its pathogenic potential and contributes to disease outcome. in order to define the transcriptional network ... | 2013 | 23250742 |
| [current pharmacotherapy of amebiasis, advances in new drugs, and design of a vaccine]. | although many drugs destroy entamoeba histolytica within the colonic lumen, the number of tissue amebicides used to treat invasive amebiasis is still relatively limited. metronidazole (mtz), which is the drug of choice for invasive amebiasis, and other nitroimidazoles have greatly simplified the chemotherapy of this disease. however, eradication of e. histolytica infection after completion of mtz therapy requires additional treatment with luminal amebicides, such as paramomycin. after decades of ... | 2012 | 23248973 |
| breast milk parasite-specific antibodies and protection from amebiasis and cryptosporidiosis in bangladeshi infants: a prospective cohort study. | in this prospective cohort study, the presence of parasite-specific immunoglobulin a in breast milk was associated with protection of bangladeshi infants from cryptosporidiosis and amebiasis. our findings suggest that passive immunity could be harnessed for the prevention of entamoeba histolytica and cryptosporidium species infection in children living in endemic regions. | 2013 | 23243179 |
| a transposon-derived dna polymerase from entamoeba histolytica displays intrinsic strand displacement, processivity and lesion bypass. | entamoeba histolytica encodes four family b2 dna polymerases that vary in amino acid length from 813 to 1279. these dna polymerases contain a n-terminal domain with no homology to other proteins and a c-terminal domain with high amino acid identity to archetypical family b2 dna polymerases. a phylogenetic analysis indicates that these family b2 dna polymerases are grouped with dna polymerases from transposable elements dubbed polintons or mavericks. in this work, we report the cloning and bioche ... | 2012 | 23226232 |
| structural determinants of ubiquitin conjugation in entamoeba histolytica. | ubiquitination is important for numerous cellular processes in most eukaryotic organisms, including cellular proliferation, development, and protein turnover by the proteasome. the intestinal parasite entamoeba histolytica harbors an extensive ubiquitin-proteasome system. proteasome inhibitors are known to impair parasite proliferation and encystation, suggesting the ubiquitin-proteasome pathway as a viable therapeutic target. however, no functional studies of the e. histolytica ubiquitination e ... | 2013 | 23209297 |
| the bifunctional aldehyde-alcohol dehydrogenase controls ethanol and acetate production in entamoeba histolytica under aerobic conditions. | by applying metabolic control analysis and inhibitor titration we determined the degree of control (flux control coefficient) of pyruvate:ferredoxin oxidoreductase (pfor) and bifunctional aldehyde-alcohol dehydrogenase (adhe) over the fluxes of fermentative glycolysis of entamoeba histolytica subjected to aerobic conditions. the flux-control coefficients towards ethanol and acetate formation determined for pfor titrated with diphenyleneiodonium were 0.07 and 0.09, whereas for adhe titrated with ... | 2013 | 23201265 |
| real-time pcr assay for rapid qualitative and quantitative detection of entamoeba histolytica. | simple real-time pcr assay with one set of primer and probe for rapid, sensitive qualitative and quantitative detection of entamoeba histolytica has been used. consensus sequences were used to amplify a species-specific region of the 16s rrna gene, and fluorescence resonance energy transfer hybridization probes were used for detection in a lightcycler platform (roche). the anchor probe sequence was designed to be a perfect match for the 16s rrna gene of entamoeba species, while the acceptor prob ... | 2012 | 23195553 |
| nitroimidazole drugs vary in their mode of action in the human parasite giardia lamblia. | giardia lamblia (syn. duodenalis, intestinalis) is a globally occurring micro-aerophilic human parasite that causes gastrointestinal disease. standard treatment of g. lamblia infections is based on the 5-nitroimidazole drugs metronidazole and tinidazole. in two other micro-aerophilic parasites, entamoeba histolytica and trichomonas vaginalis, 5-nitroimidazole drugs bind to proteins involved in the thioredoxin-mediated redox network and disrupt the redox equilibrium by inhibiting thioredoxin redu ... | 2012 | 24533278 |
| molecular epidemiology of human intestinal amoebas in iran. | many microscopic-based epidemiological surveys on the prevalence of human intestinal pathogenic and non-pathogenic protozoa including intestinal amoeba performed in iran show a high prevalence of human intestinal amoeba in different parts of iran. such epidemiological studies on amoebiasis are confusing, mainly due to recently appreciated distinction between the entamoeba histolytica, e. dispar and e. moshkovskii. differential diagnosis can be done by some methods such as pcr-based methods, mono ... | 2012 | 23193500 |
| cloning, purification, crystallization and preliminary crystallographic study of calcium-binding protein 5 from entamoeba histolytica. | entamoeba histolytica is the causative agent of human amoebiasis. phagocytosis is the major route of food intake by this parasite and is responsible for its virulence. calcium and calcium-binding proteins play major roles in its phagocytosis. calcium-binding protein 5 from e. histolytica (ehcabp5) is a cytoplasmic protein; its expression is very sensitive to serum starvation and it seems to be involved in binding to myosin i. in this study, ehcabp5 was cloned, expressed in escherichia coli and p ... | 2012 | 23192043 |
| heterotrimeric g-protein signaling is critical to pathogenic processes in entamoeba histolytica. | heterotrimeric g-protein signaling pathways are vital components of physiology, and many are amenable to pharmacologic manipulation. here, we identify functional heterotrimeric g-protein subunits in entamoeba histolytica, the causative agent of amoebic colitis. the e. histolytica gα subunit ehgα1 exhibits conventional nucleotide cycling properties and is seen to interact with ehgβγ dimers and a candidate effector, ehrgs-rhogef, in typical, nucleotide-state-selective fashions. in contrast, a crys ... | 2012 | 23166501 |
| the dynamic interdependence of amebiasis, innate immunity, and undernutrition. | entamoeba histolytica, the protozoan parasite that causes amebic dysentery, greatly contributes to disease burden in the developing world. efforts to exhaustively characterize the pathogenesis of amebiasis have increased our understanding of the dynamic host-parasite interaction and the process by which e. histolytica trophozoites transition from gut commensals to invaders of the intestinal epithelium. mouse models of disease continue to be instrumental in this area. at the same time, large-scal ... | 2012 | 23114864 |
| biochemical and functional characterization of novel nadh kinase in the enteric protozoan parasite entamoeba histolytica. | nad(h) kinase catalyzes the phosphorylation of nad(h) to form nadp(h) using atp or inorganic polyphosphate as a phosphoryl donor. while the enzyme is conserved throughout prokaryotes and eukaryotes, remarkable differences in kinetic parameters including substrate preference, cation dependence, and physiological roles exist among the organisms. in the present study, we biochemically characterized nad(h) kinase from the anaerobic/microaerophilic fermentative protozoan parasite entamoeba histolytic ... | 2013 | 23069387 |
| amoebic liver abscess in travellers: indication for image-guided puncture? | although amoebic liver abscess due to entamoeba histolytica is one of the most common parasitic infections worldwide, invasive disease remains uncommon in industrialized countries. metronidazole is the standard of care for complicated and uncomplicated invasive amoebiasis. puncture of amebic liver abscesses is a treatment option primarily for complicated abscesses (localized in left lobe, multiple, and/or pyogenic abscesses). the role of image-guided percutaneous puncture in initially uncomplica ... | 2012 | 23064858 |
| a review of amoebic liver abscess for clinicians in a nonendemic setting. | amoebic liver abscess (ala) is an uncommon but potentially life-threatening complication of infection with the protozoan parasite entamoeba histolytica. e histolytica is widely distributed throughout the tropics and subtropics, causing up to 40 million infections annually. the parasite is transmitted via the fecal-oral route, and once it establishes itself in the colon, it has the propensity to invade the mucosa, leading to ulceration and colitis, and to disseminate to distant extraintestinal si ... | 2012 | 23061067 |
| the chitin biosynthesis pathway in entamoeba and the role of glucosamine-6-p isomerase by rna interference. | entamoeba histolytica, the causative agent of amoebiasis, infects through its cyst form. a thick chitin wall protects the cyst from the harsh environment outside of the body. it is known that chitin is synthesized only during encystation, but the chitin synthesis pathway (csp) of entamoeba is not well characterized. in this report, we have identified the genes involved in chitin biosynthesis from the entamoeba genome database and verified their expression profile at the transcriptional level in ... | 2012 | 23058929 |
| src and pi3 k inhibitors affect the virulence factors of entamoeba histolytica. | protein kinases (pks) of parasitic protozoa are being evaluated as drug targets. a large number of protein kinases within the protein kinome of entamoeba histolytica strongly suggest that protein phosphorylation is a key component of pathogenesis regulation by this parasite. pi3 k and src are kinases previously described in this parasite, but their role is poorly understood. here, the effect of src-1-inhibitor and pi3 k inhibitor (wortmannin) on the virulence factors of e. histolytica was evalua ... | 2013 | 23058125 |
| loop-mediated isothermal amplification (lamp) assay for rapid detection of entamoeba histolytica in amoebic liver abscess. | amoebic liver abscess (ala) is the most common extra intestinal manifestation of invasive amoebiasis caused by entamoeba histolytica. the lack of early and accurate diagnostic test to differentiate various causes of liver abscess necessitates more reliable laboratory diagnostic test. the present study was conducted to assess the applicability of loop-mediated isothermal amplification (lamp) assay for detection of e. histolytica in ala cases. fifty patients (n = 50) with clinical suspicion of ala ... | 2013 | 23054695 |
| entamoeba histolytica rho1 regulates actin polymerization through a divergent, diaphanous-related formin. | entamoeba histolytica requires a dynamic actin cytoskeleton for intestinal and systemic pathogenicity. diaphanous-related formins represent an important family of actin regulators that are activated by rho gtpases. the e. histolytica genome encodes a large family of rho gtpases and three diaphanous-related formins, of which ehformin1 is known to regulate mitosis and cytokinesis in trophozoites. we demonstrate that ehformin1 modulates actin polymerization through its formin homology 2 domain. des ... | 2012 | 23050667 |
| feed-forward regulation of phagocytosis by entamoeba histolytica. | the parasitic protozoan entamoeba histolytica is aptly named for its capacity to destroy host tissue. when e. histolytica trophozoites invade the lamina propria of a host colon, extracellular matrices are degraded while host cells are killed and phagocytosed. the ability of e. histolytica to phagocytose host cells correlates with virulence in vivo. in order to better understand the mechanism of phagocytosis, we used an e. histolytica affymetrix microarray chip to measure the total gene expressio ... | 2012 | 23045476 |
| induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with entamoeba histolytica. | precision-cut liver slices (pcls) are mainly used to evaluate hepatotoxicity and metabolism of chemicals, as well as to study mechanisms of liver damage and repair. however, recently they have been used as a system to study amoebic infections. the aim of this study was to validate this model as an alternative for experimental amoebic liver absess (ala) in animals. to do this, the pcls was analyzed for the expression of amoebapore and cysteine proteinases 1 and 5, three of the most studied virule ... | 2012 | 23043979 |
| an unusual cause of haemoptysis: a diagnostic challenge for clinicians. | a 32-year-old male patient presented with haemoptysis in the background of high-grade fever for 3 weeks. chest examination and x-ray were suggestive of right-sided moderate pleural effusion. on finding tender hepatomegaly in abdominal examination, an ultrasonography of abdomen was performed which was suggestive of ruptured hepatic abscess. cytological examination of both sputum and aspirate from hepatic abscess showed neutrophilic debris mixed with red blood cells. the serological test for antib ... | 2012 | 23035164 |
| amebic liver abscess diagnosed by polymerase chain reaction in 14 returning travelers. | amebic liver abscesses (ala) are not commonly described in travelers. the ala diagnosis is usually based on serology and entamoeba histolytica polymerase chain reaction (pcr) is a new tool. we retrospectively reviewed all ala cases diagnosed by pcr on the liver abscess pus aspirate of patients admitted in 4 teaching hospitals in paris, france between 2007 and 2011. fourteen cases (10 male, median age 48 years) were included. the median lag time between return and onset of symptoms was 23 days am ... | 2012 | 23033402 |
| dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite entamoeba histolytica. | entamoeba histolytica, a microaerophilic enteric protozoan parasite, causes amebic colitis and extra intestinal abscesses in millions of inhabitants of endemic areas. trophozoites of e. histolytica are exposed to a variety of reactive oxygen and nitrogen species during infection. since e. histolytica lacks key components of canonical eukaryotic anti-oxidative defense systems, such as catalase and glutathione system, alternative not-yet-identified anti-oxidative defense strategies have been postu ... | 2012 | 23029590 |
| mrna decay proteins are targeted to poly(a)+ rna and dsrna-containing cytoplasmic foci that resemble p-bodies in entamoeba histolytica. | in higher eukaryotes, mrna degradation and rna-based gene silencing occur in cytoplasmic foci referred to as processing bodies (p-bodies). in protozoan parasites, the presence of p-bodies and their putative role in mrna decay have yet to be comprehensively addressed. identification of p-bodies might provide information on how mrna degradation machineries evolved in lower eukaryotes. here, we used immunofluorescence and confocal microscopy assays to investigate the cellular localization of mrna d ... | 2012 | 23029343 |
| optimization of entamoeba histolytica culturing in vitro. | entamoeba histolytica is among the most deadly parasites accounting for the second highest mortality rate among parasitic diseases. nevertheless, contrary to trypanosomatids, this protozoan in hardly studied by parasitology groups. this astonishing discrepancy is largely due to the remarkable intricate conditions required for parasite proliferation in vitro, particularly whenever large cell numbers are required. the present study was undertaken in order to optimize e. histolytica culturing harve ... | 2012 | 23026453 |
| identification of dihydropyrimidine dehydrogenase as a virulence factor essential for the survival of entamoeba histolytica in glucose-poor environments. | adaptation to nutritional changes is a key feature for successful survival of a pathogen within its host. the protozoan parasite entamoeba histolytica normally colonizes the human colon and in rare occasions, this parasite spread to distant organs, such as the liver. e. histolytica obtains most of its energy from the fermentation of glucose into ethanol. in this study, we were intrigued to know how this parasite reacts to changes in glucose availability and we addressed this issue by performing ... | 2013 | 23016994 |
| structure-function correlations of pulmonary surfactant protein sp-b and the saposin-like family of proteins. | pulmonary surfactant is a lipid-protein complex secreted by the respiratory epithelium of mammalian lungs, which plays an essential role in stabilising the alveolar surface and so reducing the work of breathing. the surfactant protein sp-b is part of this complex, and is strictly required for the assembly of pulmonary surfactant and its extracellular development to form stable surface-active films at the air-liquid alveolar interface, making the lack of sp-b incompatible with life. in spite of i ... | 2013 | 22996193 |
| entamoeba histolytica thioredoxin reductase: molecular and functional characterization of its atypical properties. | entamoeba histolytica, an intestinal protozoan that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen and nitrogen species during tissue invasion. thioredoxin reductase catalyzes the reversible transfer of reducing equivalents between nadph and thioredoxin, a small protein that plays key metabolic functions in maintaining the intracellular redox balance. | 2012 | 22967759 |
| microbiological and pharmacological evaluation of the micropropagated rubus liebmannii medicinal plant. | rubus liebmannii is an endemic species from mexico used in traditional medicine primarily to treat dysentery and cough. the in vitro activity against giardia lamblia and entamoeba histolytica that produces the ethanolic extract of the aerial parts of the plant led us to expand the pharmacological and phytochemical research of this species. gastrointestinal disorders including amebiasis remain one of the health problems that need to be addressed and it is of interest to find alternatives that imp ... | 2012 | 22966243 |
| is routine medical examination of food handlers enough to ensure food safety in hospitals? | nosocomial food outbreaks due to infected food handlers is primarily due to inadequate knowledge and faulty practices of food handlers during diarrhoeal episodes. the aim of this study was to assess: 1) prevalence of enteropathogen infection among food handlers working in our hospital during 2007 to 2011 and 2) adequacy of precautions taken by them during gastroenteritis episodes. stool samples submitted by food handlers during 2007 to 2011 were examined for the presence of enteropathogens by st ... | 2012 | 25145060 |
| sexually transmitted penile amoebiasis in iran: a case series. | four cases of penile amoebiasis (pa) presenting as genital ulceration seen among men who practiced unprotected insertive anal intercourse in tehran are described. | 2012 | 22930345 |
| intestinal parasitic infection among school children. | intestinal parasitosis is a major public health problem of developing countries, children being major victims. higher prevalence has been reported among school children, mostly in hilly regions of nepal. this study aims at assessing prevalence of intestinal parasitosis among school children of a school in a border town of nepal and the associated factors. | 2012 | 22929631 |
| entamoeba histolytica pleuropulmonary infection. case report and review of the literature. | pleuropulmonary amebiasis is the 2nd most common extraintestinal site of amebiasis after liver abscess. we describe a man with pleuropulmonary amebiasis presenting with pulmonary consolidation and pleural effusion. in patients with pneumonia coming from endemic countries such as lebanon, pleuropulmonary amebiasis should be considered in the setting of chocolate-colored sputum, negative respiratory cultures, and failure of antibacterial therapy. | 2016 | 22919872 |
| entamoeba histolytica infection in men who have sex with men. | entamoeba histolytica infection (amoebiasis) is the second leading cause of death from parasitic diseases. epidemiological studies from developed countries have reported an increasing prevalence of amoebiasis and of invasive infections, such as amoebic colitis, among men who have sex with men (msm) who engage in oral-anal sex. although most infections with e histolytica are asymptomatic, clinical manifestations of invasive amoebiasis mainly include amoebic colitis and amoebic liver abscess, whic ... | 2012 | 22917103 |
| real-time analysis of gut flora in entamoeba histolytica infected patients of northern india. | amebic dysentery is caused by the protozoan parasite entamoeba histolytica and the ingestion of quadrinucleate cyst of e. histolytica from fecally contaminated food or water initiates infection. excystation occurs in the lumen of small intestine, where motile and potentially invasive trophozoites germinate from cysts. the ability of trophozoites to interact and digest gut bacteria is apparently important for multiplication of the parasite and its pathogenicity; however the contribution of reside ... | 2012 | 22913622 |
| a functional study of nucleocytoplasmic transport signals of the ehncabp166 protein from entamoeba histolytica. | ehncabp166 is an entamoeba histolytica actin-binding protein that localizes to the nucleus and cytoplasm. bioinformatic analysis of the ehncabp166 amino acid sequence shows the presence of 3 bipartite nuclear localization signals (nls) and a nuclear export signal (nes). the present study aimed to investigate the functionality of these signals in 3 ways. first, we fused each potential nls to a cytoplasmic domain of ehfln to determine whether the localization of this domain could be altered by the ... | 2012 | 22906852 |
| sink or swim: lipid rafts in parasite pathogenesis. | lipid rafts, sterol- and sphingolipid-rich membrane microdomains, have been extensively studied in mammalian cells. recently, lipid rafts have been shown to control virulence in a variety of parasites including entamoeba histolytica, giardia intestinalis, leishmania spp., plasmodium spp., toxoplasma gondii, and trypanosoma spp. parasite rafts regulate adhesion to host and invasion, and parasite adhesion molecules often localize to rafts. parasite rafts also control vesicle trafficking, motility, ... | 2012 | 22906512 |
| flexibility of ef-hand motifs: structural and thermodynamic studies of calcium binding protein-1 from entamoeba histolytica with pb2+, ba2+, and sr2+. | ef-hand proteins can be activated by the binding of various heavy metals other than calcium, and such complexes can disturb the calcium-signaling pathway and cause toxicity and disease causing state. so far, no comprehensive study has been done to understand different heavy metals binding to calcium signaling proteins. | 2012 | 22906057 |
| a genome-wide over-expression screen identifies genes involved in phagocytosis in the human protozoan parasite, entamoeba histolytica. | functional genomics and forward genetics seek to assign function to all known genes in a genome. entamoeba histolytica is a protozoan parasite for which forward genetics approaches have not been extensively applied. it is the causative agent of amoebic dysentery and liver abscess, and infection is prevalent in developing countries that cannot prevent its fecal-oral spread. it is responsible for considerable global morbidity and mortality. given that the e. histolytica genome has been sequenced, ... | 2012 | 22905196 |
| novel pyrophosphate-forming acetate kinase from the protist entamoeba histolytica. | acetate kinase (ack) catalyzes the reversible synthesis of acetyl phosphate by transfer of the γ-phosphate of atp to acetate. here we report the first biochemical and kinetic characterization of a eukaryotic ack, that from the protist entamoeba histolytica. our characterization revealed that this protist ack is the only known member of the askha structural superfamily, which includes acetate kinase, hexokinase, and other sugar kinases, to utilize inorganic pyrophosphate (pp(i))/inorganic phospha ... | 2012 | 22903977 |
| amebic infection in humans. | clinical human infections with the protozoa entamoeba histolytica is still estimated to occur in 50 million people worldwide, of which approximately 100,000 die annually. although most clinical symptoms are due to involvement of the large intestine, 1 % present with involvement of the liver in the form of a liver abscess, a potentially fatal condition. distinguishing an invasive form (e. histolytica) from a morphologically identical non-invasive one (e. dispar) requires molecular or enzymatic ch ... | 2012 | 22903366 |
| the glyoxalase pathway in protozoan parasites. | the glyoxalase system is the main catabolic route for methylglyoxal, a non-enzymatic glycolytic byproduct with toxic and mutagenic effects. this pathway includes two enzymes, glyoxalase i and glyoxalase ii, which convert methylglyoxal to d-lactate by using glutathione as a catalytic cofactor. in protozoan parasites the glyoxalase system shows marked deviations from this model. for example, the functional replacement of glutathione by trypanothione (a spermidine-glutathione conjugate) is a charac ... | 2012 | 22901378 |
| computational prediction and validation of c/d, h/aca and eh_u3 snornas of entamoeba histolytica. | small nucleolar rnas are a highly conserved group of small rnas found in eukaryotic cells. genes encoding these rnas are diversely located throughout the genome. they are functionally conserved, performing post transcriptional modification (methylation and pseudouridylation) of rrna and other nuclear rnas. they belong to two major categories: the c/d box and h/aca box containing snornas. u3 snorna is an exceptional member of c/d box snornas and is involved in early processing of pre-rrna. an ant ... | 2012 | 22892049 |
| axenic cultivation and comparative phospholipase a2 activity of giardia duodenalis in a serum-free medium. | mammalian serum is essential for the growth of giardia duodenalis cultivated under axenic conditions. unfortunately, some factors present in bovine serum used as supplement in the culture medium may inhibit protozoal growth and activity. tyi-33-pacsr is a tyi medium supplemented with a serum replacement (pacsr) made up of earle's amino acid solution, diamond's vitamin-tween 80 mixtures and lcr (a lipid-cholesterol - rich mixture). pacsr was previously used in the culture media for axenic cultiva ... | 2012 | 22875669 |
| [prevalence of intestinal parasites at saint-camille medical center in ouagadougou (burkina faso), 1991 to 2010]. | intestinal parasitoses continue to be a major public health problem in developing countries. | 2016 | 22868724 |
| synthesis, characterization and structure optimization of a series of thiazolidinone derivatives as entamoeba histolytica inhibitors. | a series of thiazolidinone derivatives were synthesized by sodium acetate assisted cyclization of 1-isobutyl-3-phenylthiourea with chloroacetic acid followed by the piperidine facilitated substitution of the resulting thiazolidinone with different substituted aldehydes. the ethene and imine double bonds adopt (z,z) configuration as indicated by (1)h-(1)h cosy and 2d-noesy (1)h nmr and further confirmed by the crystal structure studies. the in vitro antiamoebic activity of these compounds was eva ... | 2012 | 22840694 |
| a multilocus sequence typing system (mlst) reveals a high level of diversity and a genetic component to entamoeba histolytica virulence. | the outcome of an entamoeba histolytica infection is variable and can result in either asymptomatic carriage, immediate or latent disease (diarrhea/dysentery/amebic liver abscess). an e. histolytica multilocus genotyping system based on trna gene-linked arrays has shown that genetic differences exist among parasites isolated from patients with different symptoms however, the trna gene-linked arrays cannot be located in the current assembly of the e. histolytica reference genome (strain hm-1:imss ... | 2012 | 22839995 |
| metronidazole thiosalicylate conjugates: synthesis, crystal structure, docking studies and antiamoebic activity. | metronidazole thiosalicylate conjugates were synthesized and crystallised in order to discover new molecules having better efficacy than therapeutically administered drug metronidazole, used against entamoeba histolytica. the three compounds (4-6) showed lower ic(50) values than metronidazole on hm1:imss strain of e. histolytica and displayed low cytotoxicity on mcf-7 cell line. in order to get an insight into the mechanisms of action of these compounds, a homology model of e. histolytica thiore ... | 2012 | 22832309 |
| a pentapeptide monocyte locomotion inhibitory factor protects brain ischemia injury by targeting the eef1a1/endothelial nitric oxide synthase pathway. | ischemic stroke is a major cause of death worldwide but lacks viable treatment or treatment targets. monocyte locomotion inhibitory factor (mlif) is a small heat-stable pentapeptide produced by entamoeba histolytica in axenic culture, which is supposed to protect the brain from ischemic injury; the mechanism, however, remains unknown. in this study, we further investigated the mechanism underlying the protective role of mlif in brain ischemia. | 2012 | 22829547 |
| a detoxifying oxygen reductase in the anaerobic protozoan entamoeba histolytica. | we report the characterization of a bacterial-type oxygen reductase abundant in the cytoplasm of the anaerobic protozoan parasite entamoeba histolytica. upon host infection, e. histolytica is confronted with various oxygen tensions in the host intestine, as well as increased reactive oxygen and nitrogen species at the site of local tissue inflammation. resistance to oxygen-derived stress thus plays an important role in the pathogenic potential of e. histolytica. the genome of e. histolytica has ... | 2012 | 22798391 |
| infantile amoebiasis: a case report. | amoebiasis continues to be a major cause of morbidity and mortality in children in developing countries. entamoeba histolytica infections are commonly observed in tropical and subtropical regions of the world including iran. in developed countries entamoeba histolytica infections are commonly seen in travelers, recent immigrants, homosexual men, and inmates of institutions. the disease is more severe in the two extremes of life. this paper paper describes a four-month-old male infant with entamo ... | 2012 | 22779016 |
| seroprevalence of entamoeba histolytica infection in china. | the seroprevalence of entamoeba histolytica infection in the residents of seven provinces in china was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, c-igl, of the parasites. a total of 1,312 serum samples were investigated. the positivity rates for these two antigens were 11.05% and 6.25%, respectively. there was no significant difference in the seropositivity to e. histolytica between men and women. we used a logistic regression m ... | 2012 | 22764298 |
| antiamoebic properties of the actinomycete metabolites echinomycin a and tirandamycin a. | entamoeba histolytica infects 50 million people per year, causing 100,000 deaths worldwide. the primary treatment for amoebiasis is metronidazole. however, increased pathogen resistance combined with the drug's toxic side effects encourages a search for alternative therapeutic agents. secondary metabolites from marine bacteria are a promising resource for antiprotozoan drug discovery. in this study, extracts from a collection of marine-derived actinomycetes were screened for antiamoebic properti ... | 2012 | 22763704 |
| autophosphorylation of ser428 of ehc2pk plays a critical role in regulating erythrophagocytosis in the parasite entamoeba histolytica. | the protozoan parasite entamoeba histolytica can invade both intestinal and extra intestinal tissues resulting in amoebiasis. during the process of invasion e. histolytica ingests red blood and host cells using phagocytic processes. though phagocytosis is considered to be a key virulence determinant, the mechanism is not very well understood in e. histolytica. we have recently demonstrated that a novel c2 domain-containing protein kinase, ehc2pk is involved in the initiation of erythrophagocytos ... | 2012 | 22753771 |
| an amebic anti-inflammatory peptide down-regulates ex vivo il-1β expression in patients with rheumatoid arthritis. | the monocyte locomotion inhibitory factor (mlif) is a heat-stable pentapeptide produced by entamoeba histolytica in culture. this factor displays several anti-inflammatory properties (i.e., inhibition of locomotion and respiratory burst in monocytes, reduction of skin hypersensitivity and delay of mononuclear cells in human rebuck skin windows) with inhibition of adhesion molecules, chemokines, and other genes including interleukin-1β (il-1β). in animal models, it reduces carragenin-induced infl ... | 2015 | 22749729 |
| amoebic cervicitis mimicking posterior wall fibroid: a rare presentation. | amoebiasis of the uterine cervix is an extremely rare entity, and presentation as fibroid uterus has not been reported, to the best of our knowledge in our extensive search of the english literature. it can clinically simulate cervical malignancy by virtue of surface papillomatous and overall ulcerated and necrotic appearance. we present a case of amoebic infection of the cervix in a 45-year-old female which was suspected to be a posterior wall fibroid with degeneration until a histopathological ... | 2014 | 22743593 |
| structure analysis of entamoeba histolytica dnmt2 (ehmeth). | in eukaryotes, dna methylation is an important epigenetic modification that is generally involved in gene regulation. methyltransferases (mtases) of the dnmt2 family have been shown to have a dual substrate specificity acting on dna as well as on three specific trnas (trna(asp), trna(val), trna(gly)). entamoeba histolytica is a major human pathogen, and expresses a single dna mtase (ehmeth) that belongs to the dnmt2 family and shows high homology to the human enzyme as well as to the bacterial d ... | 2012 | 22737219 |
| potential cytotoxic and amoebicide activity of first row transition metal compounds with 2,9-bis-(2',5'-diazahexanyl)-1,1-phenanthroline (l1). | a new synthetic pathway was reported to obtain n6 donor ligand 2,9-bis-(2',5'-diazahexanyl)-1,10-phenanthroline (l1) and its coordination compounds of essential divalent metal ions mn, fe, co, ni, cu and zn. complete characterization of all compounds was done with the conventional techniques. crystal structures of [nil1](pf(6))(2) and [znl1](pf(6))(2)·h(2)o were also reported. electrochemical studies have shown an active participation of the aromatic moiety of the ligand in redox reactions. the ... | 2012 | 22722265 |
| development of a multiplex pcr assay to detect gastroenteric pathogens in the feces of mexican children. | acute gastroenteritis (age) is a major cause of childhood morbidity and mortality worldwide; the etiology of age includes viruses, bacteria, and parasites. a multiplex pcr assay to simultaneously identify human astrovirus (hastv), calicivirus (hucvs), entamoeba histolytica (e. histolytica), and enteroinvasive escherichia coli (eiec) in stool samples is described. a total of 103 samples were individually analyzed by elisa (enzyme-linked immunosorbent assays) and rt-pcr/pcr. hastv and hucvs were d ... | 2012 | 22711331 |
| virulence and virulence factors in entamoeba histolytica, the agent of human amoebiasis. | human infections with entamoeba histolytica sporadically become pathogenic, unknown triggers converting the parasite to its invasive phenotype. parasite virulence results from complex host-parasite interactions implicating multiple amoebic and host factors, eliciting host defence responses and parasite resistance to stress caused by the host reactions and changing environments during tissue invasion. | 2012 | 22710276 |
| risk of underdiagnosing amebic dysentery due to false-negative entamoeba histolytica antigen detection. | entamoeba histolytica antigen assays on stool are widely used to diagnose amebiasis. we report a case of confirmed amebic colitis with a false-negative antigen detection that became positive after treatment. our results indicate that these assays may underdiagnose acute amebic infection when used alone and should be used cautiously. | 2012 | 22683092 |
| evaluation of a rapid point-of-care fecal antigen detection test for entamoeba histolytica. | amebiasis is a major cause of morbidity and mortality in the developing world. a reliable point-of-care test would help to improve diagnosis and early treatment. we evaluated a novel rapid fecal antigen detection test for e. histolytica (e. histolytica quik chek; techlab, inc., blacksburg, va), in a cohort of children in bangladesh where amebiasis is endemic. this point-of-care test had a sensitivity of 100% and a specificity of 100% when compared with enzyme-linked immunosorbent assay antigen d ... | 2012 | 22665604 |
| entamoeba histolytica: a snapshot of current research and methods for genetic analysis. | entamoeba histolytica represents one of the leading causes of parasitic death worldwide. although identified as the causative agent of amebiasis since 1875, the molecular mechanisms by which the parasite causes disease are still not fully understood. studying entamoeba reveals insights into a eukaryotic cell that differs in many ways from better-studied model organisms. thus, much can be learned from this protozoan parasite on evolution, cell biology, and rna biology. in this review we discuss s ... | 2012 | 22664276 |
| synthesis and in vitro evaluation of novel tetrazole embedded 1,3,5-trisubstituted pyrazoline derivatives as entamoeba histolytica growth inhibitors. | a series of pyrazoline derivatives (1a-15a) was synthesized by cyclization of chalcones (1-15) with 2-[5-(4-methoxyphenyl)-1h-tetrazol-1-yl]acetohydrazide under basic conditions and were screened in vitro, to find out effect on the growth of hm1: imss strain of entamoeba histolytica. the compounds 3a, 4a, 11a, 13a and 14a showed encouraging results with ic(50) value in the range of 0.86-1.28 μm. however compound 13a showed most promising results with ic(50) = 0.86 μm which is half of the metroni ... | 2012 | 22658085 |
| ccr9+ t cells contribute to the resolution of the inflammatory response in a mouse model of intestinal amoebiasis. | analysis of the mechanisms underlying the inflammatory response in amoebiasis is important to understand the immunopathology of the disease. mucosal associated effector and regulatory t cells may play a role in regulating the inflammatory immune response associated to entamoeba histolytica infection in the colon. a subpopulation of regulatory t cells has recently been identified and is characterized by the expression of the chemokine receptor ccr9. in this report, we used ccr9 deficient (ccr9(-/ ... | 2012 | 22633147 |
| genomic diversity of the human intestinal parasite entamoeba histolytica. | entamoeba histolytica is a significant cause of disease worldwide. however, little is known about the genetic diversity of the parasite. we re-sequenced the genomes of ten laboratory cultured lines of the eukaryotic pathogen entamoeba histolytica in order to develop a picture of genetic diversity across the genome. | 2012 | 22630046 |
| common pathways for receptor-mediated ingestion of escherichia coli and ldl cholesterol by entamoeba histolytica regulated in part by transmembrane kinase 39. | the single-celled parasite, entamoeba histolytica, is an enteric pathogen that ingests bacteria and host cells. inhibition of phagocytosis renders the parasite avirulent. the ligand/receptor interactions that allow e. histolytica to phagocytose are not well understood. we hypothesised that e. histolytica trophozoites might accomplish ingestion through the utilisation of a scavenger receptor for cholesterol. here we show that acetylated low density lipoprotein cholesterol was phagocytosed by amoe ... | 2012 | 22619755 |
| recombinant sines are formed at high frequency during induced retrotransposition in vivo. | non-long terminal repeat retrotransposons are referred to as long interspersed nuclear elements (lines) and their non-autonomous partners are short interspersed nuclear elements (sines). it is believed that an active sine copy, upon retrotransposition, generates near identical copies of itself, which subsequently accumulate mutations resulting in sequence polymorphism. here we show that when a retrotransposition-competent cell line of the parasitic protist entamoeba histolytica, transfected with ... | 2012 | 22617294 |
| a high-throughput drug screen for entamoeba histolytica identifies a new lead and target. | entamoeba histolytica, a protozoan intestinal parasite, is the causative agent of human amebiasis. amebiasis is the fourth leading cause of death and the third leading cause of morbidity due to protozoan infections worldwide(1), resulting in ~70,000 deaths annually. e. histolytica has been listed by the national institutes of health as a category b priority biodefense pathogen in the united states. treatment relies on metronidazole(2), which has adverse effects(3), and potential resistance of e. ... | 2012 | 22610278 |
| proteomic analysis of the cyst stage of entamoeba histolytica. | the category b agent of bioterrorism, entamoeba histolytica has a two-stage life cycle: an infective cyst stage, and an invasive trophozoite stage. due to our inability to effectively induce encystation in vitro, our knowledge about the cyst form remains limited. this also hampers our ability to develop cyst-specific diagnostic tools. | 2012 | 22590659 |
| unique short tandem repeat nucleotide sequences in entamoeba histolytica isolates from china. | a few pcr-based dna typing methods using repetitive elements contained within both protein-coding genes and noncoding dnas have been reported for entamoeba histolytica over the years. the serine-rich e. histolytica protein and trna-linked short tandem repeats (strs) are most commonly used to investigate the relationship between parasite genotype and e. histolytica infection outcome. many e. histolytica infections in china have been reported; however, little genome information has been provided. ... | 2012 | 22562237 |
| intestinal microbial community diversity between healthy and orally infected rabbit with entamoeba histolytica by eric-pcr. | enterobacterial repetitive intergenic consensus (eric)-pcr was applied to analyze the difference of intestinal microbial community diversity between healthy and orally infected rabbits with entamoeba histolytica. the dynamic changes in different parts of digestive system including the duodenum, jejunum, ileum, caecum, and rectum in healthy and infected rabbits at different time points were also tested. the intestinal microbial community of the control healthy rabbits was steady, and the total nu ... | 2012 | 22562215 |
| transient and stable transfection in the protozoan parasite entamoeba invadens. | entamoeba histolytica is an important human pathogen and a major health problem worldwide. many aspects of parasite biology can be studied with the exception of stage conversion, which cannot be reproduced adequately in e. histolytica. the reptile parasite entamoeba invadens is a vital model system for studying stage conversion since it can be induced to undergo both encystation and excystation with high efficiency in vitro. however, functional studies using e. invadens have been limited by the ... | 2012 | 22561071 |
| susceptibility to entamoeba histolytica intestinal infection is related to reduction in natural killer t-lymphocytes in c57bl/6 mice. | entamoeba histolytica is a protozoan that causes amoebiasis. recent studies demonstrated that natural killer t lymphocytes (nkt) are critical for preventing the development of amoebic liver abscess. in spite of that, there are only a handful of studies in the area. herein, we explored the role of nkt cells in e. histolytica infection using c57bl/6 wild-type and cd1(-/-) mice. animals were inoculated with e. histolytica and sacrificed 48 hours later to collect caecum samples that were used for qu ... | 2012 | 24470941 |
| withdrawn: use of a recombinant entamoeba histolytica cysteine proteinase antigen to evaluation of the efficacy of immune protective responses in miniature pigs. | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2012 | 22522180 |
| evaluation of the efficacy of a recombinant entamoeba histolytica cysteine proteinase (ehcp112) antigen in minipig. | cysteine proteinases 112 (ehcp112) of entamoeba histolytica are considered important for ameba pathogenicity. the recombinant gene was obtained by cloning and expression of the ehcp112 gene in heterologous host escherichia coli bl-21 (de3), were used to evaluate their ability to induce immune protective responses in minipig against challenge infection in a minipig-e. histolytica model. there was a 46.29% reduction (p<0.001) in the group of recovery of challenged e. histolytica compared with that ... | 2012 | 22521909 |
| exposure to host ligands correlates with colocalization of gal/galnac lectin subunits in lipid rafts and phosphatidylinositol (4,5)-bisphosphate signaling in entamoeba histolytica. | entamoeba histolytica is an intestinal parasite that causes dysentery and liver abscess. parasite cell surface receptors, such as the gal/galnac lectin, facilitate attachment to host cells and extracellular matrix. the gal/galnac lectin binds to galactose or n-acetylgalactosamine residues on host components and is composed of heavy (hgl), intermediate (igl), and light (lgl) subunits. although igl is constitutively localized to lipid rafts (cholesterol-rich membrane domains), hgl and lgl transien ... | 2012 | 22505337 |
| ehadh112 is a bro1 domain-containing protein involved in the entamoeba histolytica multivesicular bodies pathway. | ehadh112 is an entamoeba histolytica bro1 domain-containing protein, structurally related to mammalian alix and yeast bro1, both involved in the endosomal sorting complexes required for transport (escrt)-mediated multivesicular bodies (mvb) biogenesis. here, we investigated an alternative role for ehadh112 in the mvb protein trafficking pathway by overexpressing 166 amino acids of its n-terminal bro1 domain in trophozoites. trophozoites displayed diminished phagocytosis rates and accumulated exo ... | 2012 | 22500103 |
| a novel class of cysteine protease receptors that mediate lysosomal transport. | the transport of lysosomal proteins is, in general, mediated by mannose 6-phosphate receptors via carbohydrate modifications. here, we describe a novel class of receptors that regulate the transport of lysosomal hydrolases in the enteric protozoan entamoeba histolytica, which is a good model organism to investigate membrane traffic. a novel 110 kda cysteine protease (cp) receptor (cp-binding protein family 1, cpbf1) was initially discovered by affinity co-precipitation of the major cp (ehcp-a5), ... | 2012 | 22486861 |
| entamoeba histolytica cell surface calreticulin binds human c1q and functions in amebic phagocytosis of host cells. | phagocytosis of host cells is characteristic of tissue invasion by the intestinal ameba entamoeba histolytica, which causes amebic dysentery and liver abscesses. entamoeba histolytica induces host cell apoptosis and uses ligands, including c1q, on apoptotic cells to engulf them. two mass spectrometry analyses identified calreticulin in amebic phagosome preparations, and, in addition to its function as an endoplasmic reticulum chaperone, calreticulin is believed to be the macrophage receptor for ... | 2012 | 22473608 |
| purification and cellular localization of the entamoeba histolytica transcarboxylase. | genome analysis of entamoeba histolytica predicts the presence of acetyl-coa carboxylase. using western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of e. histolytica, with a molecular weight of 136 kda and biotin-carboxylase activity. this protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation. | 2012 | 22453500 |
| oral lactoferrin treatment resolves amoebic intracecal infection in c3h/hej mice. | entamoeba histolytica is a protozoan parasite that causes amoebiasis, an illness that affects many people around the world. we have previously reported that lactoferrin is able to kill e. histolytica in in vitro cultures. the aim of the present study was to evaluate the therapeutic effect of orally administered bovine lactoferrin in the control of intestinal amoebiasis of susceptible c3h/hej mice. the results showed that 20 mg lactoferrin/kg orally administered each day for 1 week was able to el ... | 2012 | 22452668 |
| lipids induce expression of serum-responsive transmembrane kinase ehtmkb1-9 in an early branching eukaryote entamoeba histolytica. | mechanisms underlying the initiation of proliferative response are known only for a few organisms, and are not understood for the medically important organisms including entamoeba histolytica. the trans membrane kinase ehtmkb1-9 of e. histolytica is one of the early indicators of proliferation and its' expression is regulated by serum, one of the components necessary for cellular proliferation in vitro. in this study we show that bovine serum albumin (bsa) can induce ehtmkb1-9 expression in plac ... | 2012 | 22451862 |
| [five year surveillance of entamoeba histolytica and giardia antigen of stool samples by elisa method]. | the aim of this study is to show the ratio of detection of distribution of e. histolytica ve giardia in the fecal samples of the patients with diarrhoea complaints admitted to the hospitals and medical centers of our instution between june 2004 and june 2009. | 2012 | 22450915 |
| evidence for a link between locus r-r sequence type and outcome of infection with entamoeba histolytica. | the results of entamoeba histolytica infections range from asymptomatic colonization to variable disease outcomes. however, markers that may predict infection outcomes are not known. here, we investigated sequence types of a non-coding trna-linked locus r-r to identify surrogate markers that may show association with infection outcomes. among 112 clinical samples--21 asymptomatic, 20 diarrhoea/dysentery and 71 liver abscesses--we identified 11 sequence types. sequence type 5rr was mostly associa ... | 2012 | 22448930 |
| thiosemicarbazone fragment embedded within 1,2,4-triazole ring as inhibitors of entamoeba histolytica. | a series of 1,2,4-triazole derivatives containing thiosemicarbazone linkage was synthesized and evaluated for their in vitro antiamoebic activity against hm1:imss strain of entamoeba histolytica. all the compounds were capable of inhibiting the growth of e. histolytica out of which four compounds (ic(50)=0.28-1.38 μm) were found to have better efficacy than the standard drug metronidazole (ic(50)=1.8 μm). cytotoxicity of the active compounds was assessed by mtt assay using human breast cancer mc ... | 2012 | 22444681 |