| new capabilities for mycosphaerella graminicola research. | mycosphaerella graminicola is a major pathogen of wheat worldwide, causing septoria leaf blotch disease. targeted gene disruption in m. graminicola, by agrobacterium tumefaciens-mediated transformation, has become an established functional genomics tool for m. graminicola research in recent years. however, in order to advance research into this economically important pathogen, further functional genomics tools need to be developed. here, we report three new capabilities for m. graminicola resear ... | 2010 | 20696006 |
| development of efficient plant regeneration and transformation system for impatiens using agrobacterium tumefaciens and multiple bud cultures as explants. | impatiens (impatiens walleriana) is a top selling floriculture crop. the potential for genetic transformation of impatiens to introduce novel flower colors or virus resistance has been limited by its general recalcitrance to tissue culture and transformation manipulations. we have established a regeneration and transformation system for impatiens that provides new alternatives to genetic improvement of this crop. | 2010 | 20696066 |
| accumulation of copper in trichoderma reesei transformants, constructed with the modified agrobacterium tumefaciens-mediated transformation technique. | a modified agrobacterium tumefaciens-mediated transformation method was established for the construction of mutants with improved copper tolerance and accumulation capability in trichoderma reesei. one transformant, at01, exhibited the highest copper accumulation capability. with copper at 0.7 mm, at01 removed 13 mg copper/g biomass (removal rate of 96%), whereas the wild-type strain removed only 6 mg copper/g biomass (removal rate of 50%). optimal conditions were a ph of 5.0 at 28°c. the pigmen ... | 2010 | 20697931 |
| mass spectrometric analysis reveals remnants of host-pathogen molecular interactions at the starch granule surface in wheat endosperm. | the starch granules of wheat seed are solar energy-driven deposits of fixed carbon and, as such, present themselves as targets of pathogen attack. the seed's array of antimicrobial proteins, peptides, and small molecules comprises a molecular defense against penetrating pathogens. in turn, pathogens exhibit an arsenal of enzymes to facilitate the degradation of the host's endosperm. in this context, the starch granule surface is a relatively unexplored domain in which unique molecular barriers m ... | 2010 | 20701481 |
| promotion of monacolin k production by agrobacterium tumefaciens-mediated transformation in monascus albidus 9901. | the binary vector pcambia3300-gpda-hph-trpc with hygromycin b phosphotransferase (hph) was constructed and transformed into monascus albidus 9901 by agrobacterium tumefaciens-mediated transformation, with gene hph as the selective marker. in order to improve the efficiency of a. tumefaciens-mediated transformation in m. albidus 9901, we optimized various factors including concentration of m. albidus 9901 spores, cell density of a. tumefaciens, co-cultivation time, temperature, and acetosyringone ... | 2011 | 20717674 |
| component identification of electron transport chains in curdlan-producing agrobacterium sp. atcc 31749 and its genome-specific prediction using comparative genome and phylogenetic trees analysis. | agrobacterium sp. atcc 31749 (formerly named alcaligenes faecalis var. myxogenes) is a non-pathogenic aerobic soil bacterium used in large scale biotechnological production of curdlan. however, little is known about its genomic information. dna partial sequence of electron transport chains (etcs) protein genes were obtained in order to understand the components of etc and genomic-specificity in agrobacterium sp. atcc 31749. degenerate primers were designed according to etc conserved sequences in ... | 2010 | 20730594 |
| [cloning of keratinocyte growth factor 2 gene (kgf2) and its transformation to brassica napus l]. | recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. in the present study, the cdna of the human gene keratinocyte growth factor 2 (kgf2) was replaced with plant preferred codons by pcr, and the modified full-length cdna was cloned into the plant expression vector pcambia-yo containing the oil-body promoter. the fusion construct pcambia-yo-kgf2 was transformed into brassica napus by agrobacterium tumefacien-mediated cotyledon transformation me ... | 2010 | 20815256 |
| [transformation of didymella bryoniae mediated by agrobacterium tumefaciens]. | gummy stem blight, a plant disease caused by didymella bryoniae, is one of the major diseases in melon. the disease can seriously reduce melon yield and quality. however, little information is available on the genetics and functional genomics of the fungal pathogen. in this study, we developed an agrobacterium-mediated transformation system for d. bryoniae by using a universal pathogenic isolate db11 and the agrobacterium tumefaciens strain c58c1 carrying plasmid pbig2rhph2 harboring the hygromy ... | 2010 | 20815261 |
| asymptomatic catheter related rhizobium radiobacter infection in a haploidentical hemapoetic stem cell recipient. | catheter related infections are reported as one of the most common source of nosocomial infections. rhizobium radibacter infections are generally manifested by fever and leukocytosis. here, a 14 months-old girl diagnosed as t (-) b (-) nk (+) severe combined immunodeficiency (scid) is presented. she had received repeated (x3) unconditioned haploidentical hematopoetic stem cell transplantations. during the follow-up, she has been arised an asymptomatic infection with r. radiobacter, which was iso ... | 2010 | 20818107 |
| [establishment of transformation system in mulberry and biosynthesis of quercetin]. | to establish the transformation system of mulberry, and test its ability of quercetin biosynthesis. | 2010 | 20822005 |
| necessary and sufficient conditions for the asymmetric synthesis of chiral amines using ω-aminotransferases. | the half reactions of ω-aminotransferase (ω-at) from vibrio fluvialis js17 (ω-atvf) were carried out using purified pyridoxal 5'-phosphate-enzyme (plp-enz) and pyridoxamine 5'-phosphate-enzyme (pmp-enz) complexes to investigate the relative activities of substrates. in the reaction generating pmp-enz from plp-enz using l-alanine as an amine donor, l-alanine showed about 70% of the initial reaction rate of (s)-α-methylbenzylamine ((s)-α-mba). however, in the subsequent half reaction recycling plp ... | 2011 | 20824676 |
| genetic transformation of lignin degrading fungi facilitated by agrobacterium tumefaciens. | white-rot fungi are primarily the major degraders of lignin, a major obstacle for commercial exploitation of plant byproducts to produce bioethanol and other industrially important products. however, to improve their efficacy for lignin degradation, it has become necessary to genetically modify these organisms using appropriate vectors. agrobacterium tumefaciens, a soil phytopathogenic bacterium, generally transforms plants by delivering a portion of the resident ti- plasmid, the t-dna (transfer ... | 2010 | 20836896 |
| characterization of liposomes formed by lipopolysaccharides from burkholderia cenocepacia, burkholderia multivorans and agrobacterium tumefaciens: from the molecular structure to the aggregate architecture. | the microstructure of liposomes formed by the lipopolysaccharides (lps) derived from burkholderia cenocepacia et-12 type strain lmg 16656, burkholderia multivorans strain c1576 and agrobacterium tumefaciens strain tt111 has been investigated by a combined experimental strategy, including dynamic light scattering (dls), small-angle neutron scattering (sans) and electron paramagnetic resonance (epr). the results highlight that the lps molecular structure determines, through a complex interplay of ... | 2010 | 20852798 |
| development of an efficient gene targeting system in colletotrichum higginsianum using a non-homologous end-joining mutant and agrobacterium tumefaciens-mediated gene transfer. | the hemibiotrophic ascomycete colletotrichum higginsianum is the casual agent of anthracnose disease of cruciferous plants. high efficiency transformation by agrobacterium tumefaciens-mediated gene transfer has been established for this fungus. however, targeted gene mutagenesis through homologous recombination rarely occurs in c. higginsianum. we have identified and disrupted the c. higginsianum homologue of the human ku70 gene, chku70, which encodes a protein that plays a role in non-homologou ... | 2010 | 20853009 |
| identification of cis- and trans-acting elements in phw126, a representative of a novel group of rolling circle plasmids. | phw126, pigrk, pigms31 and prao1 are the only known members of a novel and as yet uncharacterised family of rolling circle plasmids. phw126 contains only two open reading frames, of which one shows homology to pmv158-family mobilisation proteins. here we provide evidence that the second open reading frame encodes a replication protein (rep). mutation or deletion of this gene resulted in replication deficient constructs, but providing functional rep from a compatible vector rescued these construc ... | 2011 | 20854841 |
| over-expression of pr-10a leads to increased salt and osmotic tolerance in potato cell cultures. | the pr-10a protein (formerly sth-2) is known to be induced by biotic stress in potato. the present study demonstrates that transgenic suspension cells of the potato cultivar desiree over-expressing the pr-10a protein exhibit significantly increased salt and osmotic tolerance compared to the respective wild type cells. a comparison of the proteome pattern of solanum tuberosum suspension cultures cv. desiree before and after the treatment with nacl or sorbitol under equiosmolar conditions (740mos/ ... | 2010 | 20854851 |
| evidence of transfer by conjugation of type iv secretion system genes between bartonella species and rhizobium radiobacter in amoeba. | bartonella species cospeciate with mammals and live within erythrocytes. even in these specific niches, it has been recently suggested by bioinformatic analysis of full genome sequences that lateral gene transfer (lgt) may occur but this has never been demonstrated biologically. here we describe the sequence of the b. rattaustraliani (aust/nh4(t)) circular plasmid (pnh4) that encodes the tra cluster of the type iv secretion system (t4ss) and we eventually provide evidence that bartonella species ... | 2010 | 20856925 |
| [transformation of antimicrobial peptide fusion gene of cecropin b and rabbit np-1 to houttuynia cordata]. | to transform the antimicrobial peptide fusion gene of cecropin b and rabbit np-1(cn) into houttuynia cordata to improve its antimicrobic capability. | 2010 | 20862951 |
| the organophosphate-degrading enzyme from agrobacterium radiobacter displays mechanistic flexibility for catalysis. | the op (organophosphate)-degrading enzyme from agrobacterium radiobacter (opda) is a binuclear metallohydrolase able to degrade highly toxic op pesticides and nerve agents into less or non-toxic compounds. in the present study, the effect of metal ion substitutions and site-directed mutations on the catalytic properties of opda are investigated. the study shows the importance of both the metal ion composition and a hydrogen-bond network that connects the metal ion centre with the substrate-bindi ... | 2010 | 20868365 |
| agrobacterium tumefaciens mediated fused egfp-hph gene expression under the control of gpd promoter in pleurotus ostreatus. | a transformation system for the basidiomycete pleurotus ostreatus was established using agrobacterium-mediated infection. following p. ostreatus glyceraldehyde-3-phosphate dehydrogenase gene analysis, its promoter region including two introns was used as cis-regulatory element to drive expression of enhanced green fluorescent protein (egfp). as a selection marker, the hygromycin phosphotransferase (hph) gene cassette was used in the binary vector ppeh. mycelia without pretreatment were found to ... | 2010 | 20869218 |
| genetic basis for the hierarchical interaction between tobamovirus spp. and l resistance gene alleles from different pepper species. | the pepper l gene conditions the plant's resistance to tobamovirus spp. alleles l(1), l(2), l(3), and l(4) confer a broadening spectra of resistance to different virus pathotypes. in this study, we report the genetic basis for the hierarchical interaction between l genes and tobamovirus pathotypes. we cloned l(3) using map-based methods, and l(1), l(1a), l(1c), l(2), l(2b), and l(4) using a homology-based method. l gene alleles encode coiled-coil, nucleotide-binding, leucine-rich repeat (lrr)-ty ... | 2011 | 20879841 |
| enhanced v-atpase activity contributes to the improved salt tolerance of transgenic tobacco plants overexpressing vacuolar na(+)/h (+) antiporter atnhx1. | atnhx1, a vacuolar na(+)/h(+) antiporter gene from arabidopsis thaliana, was introduced into tobacco genome via agrobacterium tumefaciens-mediated transformation to evaluate the role of vacuolar energy providers in plants salt stress response. compared to the wild-type plants, over-expression of atnhx1 increased salt tolerance in the transgenic tobacco plants, allowing higher germination rates of seeds and successful seedling establishment in the presence of toxic concentrations of nacl. more im ... | 2010 | 20882319 |
| functional characterization of replication and stability factors of an incompatibility group p-1 plasmid from xylella fastidiosa. | xylella fastidiosa strain riv11 harbors a 25-kbp plasmid (pxf-riv11) belonging to the incp-1 incompatibility group. replication and stability factors of pxf-riv11 were identified and used to construct plasmids able to replicate in x. fastidiosa and escherichia coli. replication in x. fastidiosa required a 1.4-kbp region from pxf-riv11 containing a replication initiation gene (trfa) and the adjacent origin of dna replication (oriv). constructs containing trfa and oriv from pveis01, a related incp ... | 2010 | 20935126 |
| agrobacterium tumefaciens mediated transformation of chiv gene to trichoderma harzianum. | as a soil-borne filamentous fungus, trichoderma harzianum exhibits biological control properties because it parasitizes a large variety of phytopathogenic fungi. in this study, the vectors pbi121 and pcambia1301 and cloning vector puc18 were used to successfully construct expression vector pca-gchiv for filamentous fungi transformation mediated by agrobacterium tumefaciens.the chiv gene was successfully transferred into the biocontrol fungus t. harzianum with an efficiency of 90-110 transformant ... | 2010 | 20936373 |
| introduction of large dna inserts into the barley pathogenic fungus, ustilago hordei, via recombined binary bac vectors and agrobacterium-mediated transformation. | genetic transformation of organisms with large genome fragments containing complete genes, with regulatory elements or clusters of genes, can contribute to the functional analysis of such genes. however, large inserts, such as those found on bacterial artificial chromosome (bac) clones, are often not easy to transfer. we exploited an existing technique to convert bac clones, containing genomic dna fragments from the barley-covered smut fungus ustilago hordei to binary bacs (bibacs) to make them ... | 2010 | 20936474 |
| the structure of jann_2411 (duf1470) from jannaschia sp. at 1.45 å resolution reveals a new fold (the abate domain) and suggests its possible role as a transcription regulator. | the crystal structure of jann_2411 from jannaschia sp. strain ccs1, a member of the pfam pf07336 family classified as a domain of unknown function (duf1470), was solved to a resolution of 1.45 å by multiple-wavelength anomalous dispersion (mad). this protein is the first structural representative of the duf1470 pfam family. structural analysis revealed a two-domain organization, with the n-terminal domain presenting a new fold called the abate domain that may bind an as yet unknown ligand. the c ... | 2010 | 20944211 |
| tsukamurella tyrosinosolvens and rhizobium radiobacter sepsis presenting with septic pulmonary emboli. | clin microbiol infect abstract: septic pulmonary embolism (spe) is an uncommon, but life-threatening event that is usually associated with extrapulmonary infections. we report the first case of bilateral spe secondary to a central venous catheter-related bloodstream infection involving pathogens commonly considered environmental contaminants: tsukamurella tyrosinosolvens and rhizobium radiobacter. empirical levofloxacin treatment was confirmed by in vitro susceptibility data and produced prompt ... | 2010 | 20946410 |
| rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria. | most plasmids replicate only within a particular genus or family. | 2010 | 20949038 |
| production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures. | a synthetic bovine trypsinogen (sbtrypsinogen) was synthesized on the basis of rice-optimized codon usage via an overlap pcr strategy, prior to being expressed under the control of the sucrose starvation-inducible rice α-amylase 3d (ramy3d) promoter. secretion of trypsin into the culture medium was achieved by using the existing signal peptide. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin), mediated by agrobacterium tumefaciens. the integration of the s ... | 2010 | 20951807 |
| agrobacterium tumefaciens-mediated transformation for investigation of somatic recombination in the fungal pathogen armillaria mellea. | armillaria root disease is one of the most damaging timber and fruit tree diseases in the world. despite its economic importance, many basic questions about the biology of the causal fungi, armillaria spp., are unanswered. for example, armillaria undergoes matings between diploid and haploid mycelia, which can result in a recombinant diploid without meiosis. evidence of such somatic recombination in natural populations suggests that this reproductive mode may affect the pathogen's ecology. inves ... | 2010 | 20952653 |
| pol ii-directed short rnas suppress the nuclear export of mrna. | the synthesis and subsequent nuclear export of non-coding rna (ncrna) directed by rna polymerase (pol) ii is very sensitive to abiotic and biotic external stimuli including pathogen challenges. to assess whether stress-induced ncrnas may suppress the nuclear export of mrna, we exploited the ability of agrobacterium tumefaciens to co-deliver pol i, ii and iii promoter-based vectors for the transcription of short (s) ncrnas, gfp mrna or genomic rna of plant viruses (tobacco mosaic virus, tmv; or p ... | 2010 | 20953971 |
| upregulation of pyrophosphate: fructose 6-phosphate 1-phosphotransferase (pfp) activity in strawberry. | pyrophosphate: fructose 6-phosphate 1-phosphotransferase (pfp) is a cytosolic enzyme catalyzing the first committed step in glycolysis by reversibly phosphorylating fructose-6-phosphate to fructose-1,6-bisphosphate. the position of pfp in glycolytic and gluconeogenic metabolism, as well as activity patterns in ripening strawberry, suggest that the enzyme may influence carbohydrate allocation to sugars and organic acids. fructose-2,6-bisphosphate activates and tightly regulates pfp activity in pl ... | 2010 | 20960058 |
| diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china. | a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ... | 2010 | 20965680 |
| regeneration of transgenic plants by agrobacterium-mediated transformation of somatic embryos of juvenile and mature quercus robur. | a protocol was developed for genetic transformation of somatic embryos derived from juvenile and mature quercus robur trees. optimal transformation conditions were evaluated on the basis of the results of transient gus expression assays with five oak embryogenic lines and a strain of agrobacterium tumefaciens (eha105) harbouring a p35sgusint plasmid containing a nptii and a uida (gus) genes. for stable transformation, embryo clumps at globular/torpedo stages (4-10 mg) were inoculated with eha105 ... | 2010 | 20972795 |
| bioassays of quorum sensing compounds using agrobacterium tumefaciens and chromobacterium violaceum. | in most bacteria, a global level of regulation exists involving intercellular communication via the production and response to cell density-dependent signal molecules. this cell density-dependent regulation has been termed quorum sensing (qs). qs is a global regulator, which has been associated with a number of important features in bacteria including virulence regulation and biofilm formation. consequently, there is considerable interest in understanding, detecting, and inhibiting qs. acyl homo ... | 2011 | 21031300 |
| presence of opportunistic bacteria (rhizobium spp.) with potential for molecular misdiagnosis among canine and feline clinical samples. | rhizobium radiobacter was detected in 12 of 187 dogs and 2 of 100 cats using a polymerase chain reaction (pcr) assay formerly designed for the rickettsia genus. although pcr primers used for pathogenic infectious agents are specifically assessed to avoid cross-amplification, this retrospective study highlights the importance of sequencing to avoid molecular misdiagnosis. | 2010 | 21037894 |
| a yeast ste11 homologue comekk1 is essential for pathogenesis-related morphogenesis in colletotrichum orbiculare. | several signal transduction pathways, including mitogen-activated protein kinase (mapk) pathways, are involved in appressorium development in colletotrichum orbiculare, the causal agent of cucumber anthracnose disease. in this study, comekk1, a yeast mapk kinases (mapkk) kinase ste11 homolog, was identified as a disrupted gene in an agrobacterium tumefaciens-mediated transformation mutant. the phenotype of comekk1 disruptant was similar to that of cmk1, a saccharomyces cerevisiae fus3/kss1 mapk ... | 2010 | 21039273 |
| enhanced production of single copy backbone-free transgenic plants in multiple crop species using binary vectors with a pri replication origin in agrobacterium tumefaciens. | single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. we demonstrate that binary vectors that use a replication origin derived from the ri plasmid of agrobacterium rhizogenes (oriri) increase the frequency of single copy, backbone-free transgenic plants in agrobacterium tumefaciens mediated transformation of soybean, canola, and corn, compared to rk2-derived binary vectors (rk2 oriv). in large scale s ... | 2010 | 21042934 |
| transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity. | transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ... | 2010 | 21043054 |
| the impact of environmental contamination with antibiotics on levels of resistance in soil bacteria. | this study examined the effects of tetracycline and streptomycin on microorganisms in three different soil habitats: forest soil, agricultural soil, and compost. these antibiotics are commonly used in both medical and veterinary therapy as well as in the production of plant biomass and until quite recently, the production of animal biomass. microcosms were used as model systems in which the number of microorganisms in environments containing different amounts of antibiotics was analyzed. the min ... | 2010 | 21043273 |
| metal inducible activity of the oil palm metallothionein-like gene promoter (mt3-a) in prokaryotes. | reporter gene activity under the regulation of the oil palm metallothionein-like gene, mt3-a promoter was assessed in prokaryotes. vector constructs containing mt3-a promoter with (w1mt3-a) and without (w2mt3-a) five prime untranslated region (5'-utr) fused to ß-glucuronidase (gus) gene in pcambia 1304 vector were produced. 5'-rapid amplification of cdna ends (race) using mrna isolated from escherichia coli and agrobacterium tumefaciens harboring w1mt3-a confirmed that fusion transcripts of mt3- ... | 2010 | 21044862 |
| isolation of heat shock-induced nicotiana tabacum transcription promoters and their potential as a tool for plant research and biotechnology. | transcription promoters of heat shock protein (hsp) genes have been used to control the expression of heterologous proteins in plants and plant cells. to obtain a strong hsp promoter that is functionally active in nicotiana tabacum by-2 cells, we set out to identify a promoter of an endogenous gene showing high activation of expression by heat. an n. tabacum by-2 cell culture was treated for 8 h at 37°c and the cell protein extract analyzed by two-dimensional electrophoresis. a major spot was id ... | 2010 | 21052831 |
| investigating agrobacterium-mediated transformation of verticillium albo-atrum on plant surfaces. | agrobacterium tumefaciens has long been known to transform plant tissue in nature as part of its infection process. this natural mechanism has been utilised over the last few decades in laboratories world wide to genetically manipulate many species of plants. more recently this technology has been successfully applied to non-plant organisms in the laboratory, including fungi, where the plant wound hormone acetosyringone, an inducer of transformation, is supplied exogenously. in the natural envir ... | 2010 | 21060684 |
| structural characterization of a group ii 2/2 hemoglobin from the plant pathogen agrobacterium tumefaciens. | within the 2/2 hemoglobin sub-family, no group ii 2/2hbs from proteobacteria have been so far studied. here we present the first structural characterization of a group ii 2/2hb from the soil and phytopathogenic bacterium agrobacterium tumefaciens (at-2/2hbo). the crystal structure of ferric at-2/2hbo (reported at 2.1å resolution) shows the location of specific/unique heme distal site residues (e.g., his(42)cd1, a residue distinctive of proteobacteria group ii 2/2hbs) that surround a heme-ligande ... | 2010 | 21070893 |
| fluorescence of phytochrome adducts with synthetic locked chromophores. | we performed steady state fluorescence measurements with phytochromes agp1 and agp2 of agrobacterium tumefaciens and three mutants in which photoconversion is inhibited. these proteins were assembled with the natural chromophore biliverdin (bv), with phycoerythrobilin (peb), which lacks a double bond in the ring c-d-connecting methine bridge, and with synthetic bilin derivatives in which the ring c-d-connecting methine bridge is locked. all peb and locked chromophore adducts are photoinactive. a ... | 2010 | 21071442 |
| agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of the cultivated mushroom flammulina velutipes. | agrobacterium tumefaciens was used to transform the vegetative dikaryotic mycelium of flammulina velutipes using a hygromycin b resistance gene as selectable marker. the gene coding for urogen iii methyltransferase (cob) was introduced into f. velutipes dikaryotic cells. the resulting transformant cells generated a bright red fluorescence, indicating that cob is promising as a reporter gene in f. velutipes. | 2010 | 21071861 |
| argc orthologs from rhizobiales show diverse profiles of transcriptional efficiency and functionality in sinorhizobium meliloti. | several factors can influence ortholog replacement between closely related species. we evaluated the transcriptional expression and metabolic performance of ortholog substitution complementing a sinorhizobium meliloti argc mutant with argc from rhizobiales (agrobacterium tumefaciens, rhizobium etli, and mesorhizobium loti). the argc gene is necessary for the synthesis of arginine, an amino acid that is central to protein and cellular metabolism. strains were obtained carrying plasmids with argc ... | 2010 | 21075924 |
| qualitative and quantitative detection of agricultural microorganisms expressing iturin and mop cyclase in soils. | the environmental release of genetically engineered microorganisms (gems) to improve agriculture or remediate environmental hazards has raised concern over the fate of the organisms and their engineered genes. to detect the microorganisms released into the environment at the molecular level, bacillus subtilis kb producing iturin and pseudomonas fluorescens mx1 carrying the moc (mannityl opine catabolism) region from the agrobacterium tumefaciens were employed as model microorganisms. using speci ... | 2010 | 21077680 |
| broad-range antagonistic rhizobacteria pseudomonas fluorescens and serratia plymuthica suppress agrobacterium crown gall tumours on tomato plants. | to examine the biocontrol activity of broad-range antagonists serratia plymuthica ic1270, pseudomonas fluorescens q8r1-96 and p. fluorescens b-4117 against tumourigenic strains of agrobacterium tumefaciens and a. vitis. | 2010 | 21091861 |
| identification of virulence genes in the corn pathogen colletotrichum graminicola by agrobacterium tumefaciens-mediated transformation. | a previously developed agrobacterium tumefaciens-mediated transformation (atmt) protocol for the plant pathogenic fungus colletotrichum graminicola led to high rates of tandem integration of the whole ti-plasmid, and was therefore considered to be unsuitable for the identification of pathogenicity and virulence genes by insertional mutagenesis in this pathogen. we used a modified atmt protocol with acetosyringone present only during the co-cultivation of c. graminicola and a. tumefaciens. analys ... | 2011 | 21118348 |
| potassium chloride and rare earth elements improve plant growth and increase the frequency of the agrobacterium tumefaciens-mediated plant transformation. | plant transformation efficiency depends on the ability of the transgene to successfully interact with plant host factors. our previous work and the work of others showed that manipulation of the activity of host factors allows for increased frequency of transformation. recently we reported that exposure of tobacco plants to increased concentrations of ammonium nitrate increases the frequency of both homologous recombination and plant transgenesis. here we tested the influence of kcl and salts of ... | 2010 | 21132499 |
| [culture of hairy roots of panax japonicus and ginsenoside re synthesis]. | to establish the induction method and culture system of hairy roots of panax japonicus, and determine ginsenoside re contents. | 2010 | 21141482 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| rhizobium pusense sp. nov., isolated from rhizosphere of chickpea (cicer arietinum l). | a bacterial strain nrcpb10t was isolated from rhizosphere soil of chickpea (cicer arietinum l), in pusa, new delhi, india.16s rrna gene sequence of the strain nrcpb10t showed the highest similarity 98.9% with rhizobium radiobacter icmp 5785t followed by rhizobium larrymoorei icmp 15237t (97.7%) and rhizobium rubi lmg 17935t (97.4%). phylogenetic analysis of strain nrcpb10t using housekeeping genes reca and atpd revealed their distinct position differing from other recognized rhizobium species. d ... | 2010 | 21148676 |
| a glutamic acid-rich protein identified in verticillium dahliae from an insertional mutagenesis affects microsclerotial formation and pathogenicity. | verticillium dahliae kleb. is a phytopathogenic fungus that causes wilt disease in a wide range of crops, including cotton. the life cycle of v. dahliae includes three vegetative phases: parasitic, saprophytic and dormant. the dormant microsclerotia are the primary infectious propagules, which germinate when they are stimulated by root exudates. in this study, we report the first application of agrobacterium tumefaciens-mediated transformation (atmt) for construction of insertional mutants from ... | 2010 | 21151869 |
| development of a transgenic hairy root system in jute (corchorus capsularis l.) with gusa reporter gene through agrobacterium rhizogenes mediated co-transformation. | transgenic hairy root system is important in several recalcitrant plants, where agrobacterium tumefaciens-mediated plant transformation and generation of transgenic plants are problematic. jute (corchorus spp.), the major fibre crop in indian subcontinent, is one of those recalcitrant plants where in vitro tissue culture has provided a little success, and hence, agrobacterium-mediated genetic transformation remains to be a challenging proposition in this crop. in the present work, a system of tr ... | 2010 | 21153028 |
| molecular insights into plant cell proliferation disturbance by agrobacterium protein 6b. | the agrobacterium ti plasmid (t-dna) 6b proteins interact with many different host proteins implicated in plant cell proliferation. here, we show that arabidopsis plants overexpressing 6b display microrna (mirna) deficiency by directly targeting serrate and ago1 via a specific loop fragment (residues 40-55). in addition, we report the crystal structures of agrobacterium tumefaciens ak6b at 2.1 å, agrobacterium vitis ab6b at 1.65 å, and arabidopsis adp ribosylation factor (arf) at 1.8 å. the 6b s ... | 2010 | 21156810 |
| an efficient foxtail mosaic virus vector system with reduced environmental risk. | plant viral vectors offer high-yield expression of pharmaceutical and commercially important proteins with a minimum of cost and preparation time. the use of agrobacterium tumefaciens has been introduced to deliver the viral vector as a transgene to each plant cell via a simple, nonsterile infiltration technique called "agroinoculation". with agroinoculation, a full length, systemically moving virus is no longer necessary for excellent protein yield, since the viral transgene is transcribed and ... | 2010 | 21162736 |
| reactivation of meristem activity and sprout growth in potato tubers require both cytokinin and gibberellin. | reactivation of dormant meristems is of central importance for plant fitness and survival. due to their large meristem size, potato (solanum tuberosum) tubers serve as a model system to study the underlying molecular processes. the phytohormones cytokinins (ck) and gibberellins (ga) play important roles in releasing potato tuber dormancy and promoting sprouting, but their mode of action in these processes is still obscure. here, we established an in vitro assay using excised tuber buds to study ... | 2010 | 21163959 |
| gene encoding a c-type cyclin in mycosphaerella graminicola is involved in aerial mycelium formation, filamentous growth, hyphal swelling, melanin biosynthesis, stress response, and pathogenicity. | mycosphaerella graminicola is an important wheat pathogen causing septoria tritici blotch. to date, an efficient strategy to control m. graminicola has not been developed. more significantly, we have a limited understanding of the molecular mechanisms of m. graminicola pathogenicity. in this study, we attempted to characterize an mcc1-encoding c-type cyclin, a gene homologous to fcc1 in fusarium verticillioides. four independent mcc1 knock-out mutants were generated via agrobacterium tumefaciens ... | 2011 | 21171890 |
| development of antibiotic marker-free creeping bentgrass resistance against herbicides. | herbicide-resistant creeping bentgrass plants (agrostis stolonifera l.) without antibiotic-resistant markers were produced by agrobacterium-mediated transformation. embryogenic callus tissues were infected with agrobacterium tumefaciens eha105, harboring the bar and the cp4-epsps genes for bialaphos and glyphosate resistance. phosphinothricin-resistant calli and plants were selected. soil-grown plants were obtained at 14-16 weeks after transformation. genetic transformation of the selected, rege ... | 2011 | 21173055 |
| agrobacterium-mediated silencing of caffeine synthesis through root transformation in camellia sinensis l. | tea [camellia sinensis (l.) o. kuntze] is a perennial and most popular non-alcoholic caffeine-containing beverage crop. tea has several constraints for its genetic improvement such as its high polyphenolic content and woody perennial nature. the development of transgenic tea is very difficult, laborious, and time taking process. in tea, regeneration requires minimum 8-12 months. in view of this, attempt has been made in this article to develop a rapid, efficient, and quite economical agrobacteri ... | 2010 | 21181507 |
| production of marker-free disease-resistant potato using isopentenyl transferase gene as a positive selection marker. | the use of antibiotic or herbicide resistant genes as selection markers for production of transgenic plants and their continuous presence in the final transgenics has been a serious problem for their public acceptance and commercialization. mat (multi-auto-transformation) vector system has been one of the different strategies to excise the selection marker gene and produce marker-free transgenic plants. in the present study, ipt (isopentenyl transferase) gene was used as a selection marker gene. ... | 2010 | 21184230 |
| control of lipopolysaccharide biosynthesis by ftsh-mediated proteolysis of lpxc is conserved in enterobacteria but not in all gram-negative bacteria. | despite the essential function of lipopolysaccharides (lps) in gram-negative bacteria, it is largely unknown how the exact amount of this molecule in the outer membrane is controlled. the first committed step in lps biosynthesis is catalyzed by the lpxc enzyme. in escherichia coli, the cellular concentration of lpxc is adjusted by the only essential protease in this organism, the membrane-anchored metalloprotease ftsh. turnover of e. coli lpxc requires a length- and sequence-specific c-terminal ... | 2010 | 21193611 |
| biochemical characteristics of the novel haloalkane dehalogenase data, isolated from the plant pathogen agrobacterium tumefaciens c58. | we report the biochemical characterization of a novel haloalkane dehalogenase, data, isolated from the plant pathogen agrobacterium tumefaciens c58. data possesses a peculiar pair of halide-stabilizing residues, asn-tyr, which have not been reported to play this role in other known haloalkane dehalogenases. data has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chi ... | 2010 | 21193677 |
| agrobacterium tumefaciens-mediated transgenic plant production via direct shoot bud organogenesis from pre-plasmolyzed leaf explants of catharanthus roseus. | production of agrobacterium tumefaciens-mediated transgenic plants, via direct shoot bud organogenesis from leaves of catharanthus roseus, is reported. a. tumefaciens harbouring the plasmid pbi121 with gus gene uida and kanamycin resistance gene nptii was used. highest transformation efficiency of 1.4 transgenic shoots/responded explant was obtained when pre-plasmolysed leaves, pre-incubated on shoot bud induction medium for 10 days, were subjected to sonication for 30 s prior to transformation. ... | 2011 | 21207108 |
| biolistic-mediated transformation protocols for maize and pearl millet using pre-cultured immature zygotic embryos and embryogenic tissue. | maize (zea mays l.) is the most important cereal food crop in sub-saharan africa and latin america, and a key feed crop in asia, whereas pearl millet (pennisetum glaucum (l.) r. br.) is a staple food that supplies a major proportion of calories and protein to large segments of the populations living in the semi-arid tropical regions of africa and asia. the limitations of biological gene transfer with agrobacterium tumefaciens specifically related to recalcitrant cereal crops, led to the developm ... | 2011 | 21207279 |
| agrobacterium tumefaciens-mediated genetic transformation of cereals using immature embryos. | a critical step in the development of a robust agrobacterium tumefaciens-mediated transformation -system for cereal crop plants is the establishment of optimal conditions for efficient t-dna delivery into target tissue, from which plants can be regenerated. although, agrobacterium-mediated transformation of cereals is an important method that has been widely used by many laboratories around the world, routine protocols have been established only in specific cultivars within a species and with sp ... | 2011 | 21207280 |
| highly efficient agrobacterium-mediated transformation of banana cv. rasthali (aab) via sonication and vacuum infiltration. | a reproducible and efficient transformation method was developed for the banana cv. rasthali (aab) via agrobacterium-mediated genetic transformation of suckers. three-month-old banana suckers were used as explant and three agrobacterium tumefaciens strains (eha105, eha101, and lba4404) harboring the binary vector pcambia1301 were used in the co-cultivation. the banana suckers were sonicated and vacuum infiltered with each of the three a. tumefaciens strains and co-cultivated in the medium contai ... | 2011 | 21212957 |
| the integrity of the periplasmic domain of the vira sensor kinase is critical for optimal coordination of the virulence signal response in agrobacterium tumefaciens. | the plant pathogen agrobacterium tumefaciens responds to three main signals at the plant-bacterium interface: phenolics, such as acetosyringone (as), monosaccharides, and acidic ph (∼5.5). these signals are transduced via the chromosomally encoded sugar binding protein chve and the ti plasmid-encoded vira/virg two-component regulatory system, resulting in the transcriptional activation of the ti plasmid virulence genes. here, we present genetic and physical evidence that the periplasmic domain o ... | 2011 | 21216996 |
| agrobacterium tumefaciens-mediated transformation of a taxol-producing endophytic fungus, cladosporium cladosporioides md2. | in this study, an agrobacteriurn tumefaciens-mediated transformation (atmt) protocol was successfully developed for the genetic transformation of a taxol-producing fungus, cladosporium cladosporioides md2, and the co-cultivation conditions affecting the transformation efficiency were optimized. the optimal transformation conditions were that 1 ml of c. cladosporioides md2 spore suspension (10(8) spores/ml) was mixed with an equal volume of a. tumefaciens cultures, which contained 400 μl of a. tu ... | 2011 | 21221971 |
| biotechnology tools in agriculture: recent patents involving soybean, corn and sugarcane. | the technological opportunities opened up by biotechnology in agriculture are diverse, including plant breeding, the partial or total relief of pesticides chemicals usage, the improvement of soil fertility, the improvement of the quality attributes of various foods. specifically, various tricks of biotechnology can be used for higher seed yield, resistance to diseases and insects, better stems and roots, tolerance to drought and heat, and better agronomic quality. a number of recent works consid ... | 2011 | 21226663 |
| rnai-mediated down-regulation of ornithine decarboxylase (odc) leads to reduced nicotine and increased anatabine levels in transgenic nicotiana tabacum l. | in leaf and root tissues of nicotiana tabacum l. (common tobacco), nicotine is by far the predominant pyridine alkaloid, with anatabine representing only a minor component of the total alkaloid fraction. the pyrrolidine ring of nicotine is derived from the diamine putrescine, which can be synthesized either directly from ornithine via the action of odc, or from arginine via a three enzymatic step process, initiated by adc. previous studies in this laboratory have shown that antisense-mediated do ... | 2011 | 21232776 |
| homologous overexpression of xylanase in fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (cbp) of lignocellulosics. | in an effort to increase ethanol productivity during the consolidated bioprocessing (cbp) of lignocellulosics by fusarium oxysporum, we attempted the constitutive homologous overexpression of one of the key process enzymes, namely an endo-xylanase. the endo-β-1,4-xylanase 2 gene was incorporated into the f. oxysporum genome under the regulation of the gpda promoter of aspergillus nidulans. the transformation was effected through agrobacterium tumefaciens and resulted in 12 transformants, two of ... | 2011 | 21237221 |
| catalytic mechanism and three-dimensional structure of adenine deaminase. | adenine deaminase (ade) catalyzes the conversion of adenine to hypoxanthine and ammonia. the enzyme isolated from escherichia coli using standard expression conditions was low for the deamination of adenine (k(cat) = 2.0 s(-1); k(cat)/k(m) = 2.5 × 10(3) m(-1) s(-1)). however, when iron was sequestered with a metal chelator and the growth medium was supplemented with mn(2+) prior to induction, the purified enzyme was substantially more active for the deamination of adenine with k(cat) and k(cat)/ ... | 2011 | 21247091 |
| agrobacterium tumefaciens and a. rhizogenes use different proteins to transport bacterial dna into the plant cell nucleus. | agrobacterium tumefaciens and a. rhizogenes transport single-stranded dna (ssdna; t-strands) and virulence proteins into plant cells through a type iv secretion system. dna transfer initiates when vird2 nicks border sequences in the tumour-inducing plasmid, attaches to the 5' end, and pilots t-strands into plant cells. agrobacterium tumefaciens translocates ssdna-binding protein vire2 into plant cells where it targets t-strands into the nucleus. some a. rhizogenes strains lack vire2 but transfer ... | 2009 | 21255274 |
| the outer membrane protein tolc is required for phytoalexin resistance and virulence of the fire blight pathogen erwinia amylovora. | erwinia amylovora causes fire blight on several plant species such as apple and pear, which produce diverse phytoalexins as defence mechanisms. an evolutionary successful pathogen thus must develop resistance mechanisms towards these toxic compounds. the e. amylovora outer membrane protein, tolc, might mediate phytoalexin resistance through its interaction with the multidrug efflux pump, acrab. to prove this, a tolc mutant and an acrb/tolc double mutant were constructed. the minimal inhibitory c ... | 2009 | 21255278 |
| colonization process of olive tissues by verticillium dahliae and its in planta interaction with the biocontrol root endophyte pseudomonas fluorescens picf7. | the colonization process of olea europaea by the defoliating pathotype of verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain pseudomonas fluorescens picf7 were determined. differential fluorescent protein tagging was used for the simultaneous visualization of p. fluorescens picf7 and v. dahliae in olive tissues. olive plants were bacterized with picf7 and then transferred to v. dahliae-infested soil. monitoring olive colonization events by v. dahliae and i ... | 2009 | 21255281 |
| efficient insertional mutagenesis system for the dimorphic pathogenic fungus sporothrix schenckii using agrobacterium tumefaciens. | sporothrix schenckii is a dimorphic pathogenic fungus that causes human and animal sporotrichosis globally. here we developed and optimized an agrobacterium tumefaciens-mediated transformation (atmt) system of s. schenckii for insertional mutagenesis. the transformation efficiency reached more than 600 transformants per 10(6) conidia. using this protocol enabled us to obtain a large number of t-dna insertional mutants within a short experimental period. several mutants with altered phenotypes we ... | 2011 | 21256883 |
| [cotransformation of aspen and birch with three t-dna regions from two different replicons in one agrobacterium tumefaciens strain]. | the cointegration rate into the aspen and birch genomes of foreign genes from a binary vector and a disarmed ti plasmid pcbe21 carried by the same agrobacterium tumefaciens strain was studied. the cotransformation rate for the genes within the ti plasmid varied from 30 to 100%; while the transformation rate for the gene from t(l) region was twofold higher as compared with the t(r) region. on the average, the gene transfer from all three t-dnas was recorded in 10.9% of the transgenic lines. for t ... | 2010 | 21261057 |
| pseudomonas aeruginosa pao1 virulence factors and poplar tree response in the rhizosphere. | whole-transcriptome analysis was used here for the first time in the rhizosphere to discern the genes involved in the pathogenic response of pseudomonas aeruginosa pao1 as well as to discern the response of the poplar tree. differential gene expression shows that 185 genes of the bacterium and 753 genes of the poplar tree were induced in the rhizosphere. using the p. aeruginosa transcriptome analysis, isogenic knockout mutants, and two novel plant assays (poplar and barley), seven novel pao1 vir ... | 2008 | 21261818 |
| pseudomonas aeruginosa pao1 virulence factors and poplar tree response in the rhizosphere. | whole-transcriptome analysis was used here for the first time in the rhizosphere to discern the genes involved in the pathogenic response of pseudomonas aeruginosa pao1 as well as to discern the response of the poplar tree. differential gene expression shows that 185 genes of the bacterium and 753 genes of the poplar tree were induced in the rhizosphere. using the p. aeruginosa transcriptome analysis, isogenic knockout mutants, and two novel plant assays (poplar and barley), seven novel pao1 vir ... | 2008 | 21261818 |
| t-dna insertion, plasmid rescue and integration analysis in the model mycorrhizal fungus laccaria bicolor. | ectomycorrhiza is a mutualistic symbiosis formed between fine roots of trees and the mycelium of soil fungi. this symbiosis plays a key role in forest ecosystems for the mineral nutrition of trees and the biology of the fungal communities associated. the characterization of genes involved in developmental and metabolic processes is important to understand the complex interactions that control the ectomycorrhizal symbiosis. agrobacterium-mediated gene transfer (amt) in fungi is currently opening ... | 2008 | 21261845 |
| exploration of twin-arginine translocation for expression and purification of correctly folded proteins in escherichia coli. | historically, the general secretory (sec) pathway of gram-negative bacteria has served as the primary route by which heterologous proteins are delivered to the periplasm in numerous expression and engineering applications. here we have systematically examined the twin-arginine translocation (tat) pathway as an alternative, and possibly advantageous, secretion pathway for heterologous proteins. overall, we found that: (i) export efficiency and periplasmic yield of a model substrate were affected ... | 2008 | 21261860 |
| atrazine biodegradation in the lab and in the field: enzymatic activities and gene regulation. | atrazine is an herbicide of the s-triazine family that is used primarily as a nitrogen source by degrading microorganisms. while many catabolic pathways for xenobiotics are subjected to catabolic repression by preferential carbon sources, atrazine utilization is repressed in the presence of preferential nitrogen sources. this phenomenon appears to restrict atrazine elimination in nitrogen-fertilized soils by indigenous organisms or in bioaugmentation approaches. the mechanisms of nitrogen contro ... | 2009 | 21261912 |
| denitrification regulatory phenotype, a new term for the characterization of denitrifying bacteria. | current knowledge of denitrification is based on detailed studies of a limited number of organisms. in most cases the importance of these paradigm species in natural ecosystems is questionable. detailed phenotypic studies of a wider range of prokaryotes, both type strains and dominant denitrifiers isolated from complex systems, will aid the generation of more sophisticated mathematical models for the prediction of no and n2o emission to the environment. however, in order to facilitate the compar ... | 2011 | 21265774 |
| er disruption and gfp degradation during non-regenerable transformation of flax with agrobacterium tumefaciens. | flax is considered as plant species susceptible to agrobacterium-mediated genetic transformation. in this study, stability of flax transformation by agrobacterium rhizogenes versus agrobacterium tumefaciens was tested by using combined selection for antibiotic resistance and visual selection of green fluorescent protein (gfp)-fusion reporter targeted to the endoplasmic reticulum (er). transformation with a. rhizogenes was stable for over 2 years, whereas transformation by a. tumefaciens resulted ... | 2011 | 21267608 |
| virus-derived transgenes expressing hairpin rna give immunity to tobacco mosaic virus and cucumber mosaic virus. | an effective method for obtaining resistant transgenic plants is to induce rna silencing by expressing virus-derived dsrna in plants and this method has been successfully implemented for the generation of different plant lines resistant to many plant viruses. | 2011 | 21269519 |
| excision and episomal replication of cauliflower mosaic virus integrated into a plant genome. | transgenic arabidopsis (arabidopsis thaliana) plants containing a monomeric copy of the cauliflower mosaic virus (camv) genome exhibited the generation of infectious, episomally replicating virus. the circular viral genome had been split within the nonessential gene ii for integration into the arabidopsis genome by agrobacterium tumefaciens-mediated transformation. transgenic plants were assessed for episomal infections at flowering, seed set, and/or senescence. the infections were confirmed by ... | 2011 | 21278309 |
| increased resistance to cucumber mosaic virus (cmv) in lilium transformed with a defective cmv replicase gene. | lilium cv acapulco was transformed with a defective cucumber mosaic virus (cmv) replicase gene (cmv2-gdd) construct using agrobacterium tumefaciens. four lines were analyzed for gene expression and resistance to cmv-o strain. expression of the cmv2-gdd gene in the transgenic plants was confirmed by reverse transcription pcr (rt-pcr). when these four lines were mechanically inoculated with cmv-o, no signal of coat protein (cp) messages using rt-pcr was detected in newly produced leaves of two tra ... | 2011 | 21287228 |
| homology modelling and site-directed mutagenesis studies of the epoxide hydrolase from phanerochaete chrysosporium. | three-dimensional structural model of epoxide hydrolase (pcheha) from phanerochaete chrysosporium was constructed based on x-ray structure of agrobacterium radiobacter ad1 seh using swiss-model server. conserved residues constituting the active site cavity were identified, of which the functional roles of 14 residues were determined by site-directed mutagenesis. in catalytic triad, asp105 and his308 play a leading role in alkylation and hydrolysis steps, respectively. distance between asp105 and ... | 2011 | 21292804 |
| development of disease-resistant marker-free tomato by r/rs site-specific recombination. | the selection marker genes, imparting antibiotic or herbicide resistance, in the final transgenics have been criticized by the public and considered a hindrance in their commercialization. multi-auto-transformation (mat) vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators (pgrs). in the study reported here, isopentenyltransferase (ipt) gene was used as a selection marker and wasabi defensin (wd) gene, ... | 2011 | 21293863 |
| insertional mutagenesis combined with an inducible filamentation phenotype reveals a conserved ste50 homologue in cryptococcus neoformans that is required for monokaryotic fruiting and sexual reproduction. | cryptococcus neoformans typically grows in a yeast-like morphology; however, under specific conditions the fungus can produce hyphae that are either dikaryotic or monokaryotic. in this study, we developed a simple method for inducing robust monokaryotic fruiting and combined the assay with agrobacterium tumefaciens insertional mutagenesis to screen for hyphal mutants. a c. neoformans homologue of the saccharomyces cerevisiae ste50 gene was identified and characterized. ste50 was found to be requ ... | 2011 | 21299652 |
| efficient sweet pepper transformation mediated by the baby boom transcription factor. | pepper (capsicum l.) is a nutritionally and economically important crop that is cultivated throughout the world as a vegetable, condiment, and food additive. genetic transformation using agrobacterium tumefaciens (agrobacterium) is a powerful biotechnology tool that could be used in pepper to develop community-based functional genomics resources and to introduce important agronomic traits. however, pepper is considered to be highly recalcitrant for agrobacterium-mediated transformation, and curr ... | 2011 | 21305301 |
| it is a long way to gm agriculture. | when we discovered that crown gall induction on plants by agrobacterium tumefaciens is a natural event of genetic engineering, we were convinced that this was the dawn of a new era for plant science. now, more than 30 years later, i remain overawed by how far and how rapidly we progressed with our knowledge of the molecular basis of plant growth, development, stress resistance, flowering, and ecological adaptation, thanks to the gene engineering technology. i am impressed, but also frustrated by ... | 2011 | 21314429 |
| immunogenicity of nuclear-encoded ltb:st fusion protein from escherichia coli expressed in tobacco plants. | enterotoxigenic escherichia coli (etec) is one of the main causative agents of diarrhea in infants and for travelers. inclusion of a heat-stable (st) toxin into vaccine formulations is mandatory as most etec strains can produce both heat-labile (lt) and st enterotoxins. in this study, a genetic fusion gene encoding for an ltb:st protein has been constructed and transferred into tobacco via agrobacterium tumefaciens-mediated transformation. transgenic tobacco plants carrying the ltb:st gene are t ... | 2011 | 21318355 |
| small rna-mediated control of the agrobacterium tumefaciens gaba binding protein. | wounded plants activate a complex defence programme in response to agrobacterium tumefaciens. they synthesize the non-proteinogenic amino acid ?-aminobutyric acid (gaba), which stimulates degradation of the quorum sensing signal n-(3-oxo-octanoyl) homoserine lactone. gaba is transported into a. tumefaciens via an abc transporter dependent on the periplasmic binding protein atu2422. we demonstrate that expression of atu2422 and two other abc transporter genes is downregulated by the conserved sma ... | 2011 | 21320185 |
| electrochemical detection of quorum sensing signaling molecules by dual signal confirmation at microelectrode arrays. | n-acyl homoserine lactones (ahls) are produced by gram-negative bacteria to regulate gene expression in a cell density dependent manner. for instance, expression of virulence factors by pathogens such as pseudomonas aeruginosa is induced only when a threshold concentration of ahls is reached, which indicates that the bacterial population is big enough to promote infection. in this study, the indicator strain agrobacterium tumefaciens ntl4 (pzlr4), which carries a ß-galactosidase (ß-gal) reporter ... | 2011 | 21323339 |
| efficient agrobacterium-based transient expression system for the production of biopharmaceuticals in plants. | we have recently described an efficient transient expression system mediated by agrobacterium tumefaciens for the production of hiv-1 nef protein in nicotiana benthamiana plants. in order to enhance the yield of recombinant protein we assayed the effect of three gene-silencing viral suppressor proteins (p25 of potato virus x, p19 of artichoke mottled crinckle virus and tomato bushy stunt virus) on nef expression levels. results demonstrated that amcv-p19 gave the highest nef yield (1.3% of total ... | 2010 | 21326930 |
| bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications. | distinct from most alginate-assimilating bacteria that secrete polysaccharide lyases extracellularly, a gram-negative bacterium, sphingomonas sp. a1 (strain a1), can directly incorporate alginate into its cytoplasm, without degradation, through a "superchannel" consisting of a mouth-like pit on the cell surface, periplasmic binding proteins, and a cytoplasmic membrane-bound atp-binding cassette transporter. flagellin homologues function as cell surface alginate receptors essential for expressing ... | 2010 | 21326935 |