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gallbladder ascariasis.background/aims: gallbladder ascariasis is rarely encountered. the causative agent is ascaris lumbricoides. presence of ascaris inside the gallbladder can incite acalculous cholecystitis. we aimed in this report to study gallbladder ascariasis. methods: this is a case study of 28 patients with a diagnosis of gallbladder ascariasis from 2005 to 2010 who were seen in the department of general surgery, smhs hospital. results: three patients with gallbladder ascariasis had associated worms in the co ...201121796555
biological control of ascaris suum eggs by pochonia chlamydosporia fungus.ascaris suum is a gastrointestinal nematode parasite of swines. the aim of this study was to observe pochonia chlamydosporia fungus on biological control of a. suum eggs after fungus passage through swines gastrointestinal tract. eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate vc4; group 2, treated with the fungus isolate vc1 and group 3 did not receive fungus (control). in the treated groups, each animal received a 9 g single ...201121796329
factors associated with high prevalence of intestinal protozoan infections among patients in sana'a city, yemen.intestinal protozoan diseases in yemen are a significant health problem with prevalence ranging from 18% to 27%. the present study is a cross-sectional study aimed at determining the factors associated with the high prevalence of intestinal protozoan infections among patients seeking health care in sana'a city, the capital of yemen.201121789210
molecular and catalytic properties of an arginine kinase from the nematode ascaris suum.we amplified the cdna coding for arginine kinase (ak) from the parasitic nematode ascaris suum, cloned it in pmal plasmid and expressed the enzyme as a fusion protein with the maltose-binding protein. the whole cdna was 1260 bp, encoding 400 amino acids, and the recombinant protein had a molecular mass of 45,341 da. ascaris suum recombinant ak showed significant activity and strong affinity ( k _{m}^{arg} = 0.126\hairsp mm) for the substrate l-arginine. it also exhibited high catalytic efficienc ...201121781373
[investigation of intestinal parasites among elementary school students in the mugla province].objective: in this study prevalence of intestinal parasite agents was investigated among elementary school students in the mugla city centre. methods: only 663 relatives among 3560 students allowed us to investigate and obtain fecal specimens of the students. specimens were examined by native and lugol staining. repeated samples were requested from suspected cases and evaluated. results: intestinal parasites were found in 73 stool specimens (11.01%) taken from students. the frequently observed p ...201121776594
quantitative phase restoration by direct inversion using the optical transfer function.quantitative phase recovery of phase objects is achieved by a direct inversion using the defocused weak object transfer function. the presented method is noniterative and is based on partially coherent principles. it also takes into account the optical properties of the system and gives the phase of the object directly. the proposed method is especially suitable for application to weak phase objects, such as live and unstained biological samples but, surprisingly, has also been shown to work wit ...201121765504
new insights into the mechanism of fertilization in nematodes.fertilization results from the fusion of male and female gametes in all sexually reproducing organisms. much of nematode fertility work was focused on caenorhabditis elegans and ascaris suum. the c. elegans hermaphrodite produces a limited number of sperm initially and then commits to the exclusive production of oocytes. the postmeiotic differentiation called spermiogenesis converts sessile spermatids into motile spermatozoa. the motility of spermatozoa depends on dynamic assembly and disassembl ...201121749902
intestinal parasites isolated in a large teaching hospital, italy, 1 may 2006 to 31 december 2008.intestinal parasites account for the majority of parasitic diseases, particularly in endemic areas. most are transmitted via contaminated food. because of increased immigration and travel, enteric parasitoses are now distributed worldwide. between may 2006 and december 2008, we examined stool specimens from 5,351 patients (4,695 italians, 656 non-italians) for ova and parasites using microscopy, culture techniques, and molecular methods. stools from 594 patients (11.1%) were contaminated and for ...201121699767
soil-transmitted helminthiasis in rural south-west china: prevalence, intensity and risk factor analysis.only few studies in rural china have explored the epidemiology of intestinal helminth infections and identified risk factors for transmission. the study was carried out in simao and mengla counties, where single fecal samples were collected from 317 school-aged children and from 94 inhabitants of a single village. fecal specimens were examined with the kato-katz thick smear method and examined for helminth eggs. data regarding socio-demographic and behavioral risk factors were collected using qu ...201121706929
soil transmitted helminths and schistosoma mansoni infections among school children in zarima town, northwest ethiopia.in ethiopia, because of low quality drinking water supply and latrine coverage, helminths infections are the second most predominant causes of outpatient morbidity. indeed, there is a scarcity of information on the prevalence of soil transmitted helminths and schistosomiasis in ethiopia, special in study area. therefore, the aim of this study was to determine the prevalence and associated risk factors of soil transmitted helminths and intestinal schistosomiasis.201121740589
cryptosporidium and other enteric parasitic infections in hiv-seropositive individuals with and without diarrhoea in osogbo, nigeria.the primary objective of this cross-sectional study is to correlate the presence of cryptosporidium and other gastrointestinal parasites with the presence of diarrhoea in human immunodeficiency virus (hiv)-infected patients. stool samples from 96 hiv-seropositive cases were examined for non-opportunistic parasites using the direct and formol-ether concentration methods, while the modified ziehl-neelsen technique was used to detect cryptosporidium spp. the overall prevalence of cryptosporidium sp ...201121706918
recurrent episodes of upper airway blockage associated with ascaris lumbricoides causing cardiopulmonary arrest in a young patient.ascaris lumbricoides (al) is one of the most common helminthic infections of humans, affecting mainly children and malnourished individuals residing in developing countries. it is estimated the 25% of the world's population is infected with this nematode. infection occurs via ingestion of water and food contaminated by ascaris eggs, causing asymptomatic infection to disease with abdominal pain, vomiting, constipation and faecal passage of worms. the present report concerns an unusual case in whi ...200921687030
synthesis of a new class of ribose functionalized dinucleotide cap analogues for biophysical studies on interaction of cap-binding proteins with the 5' end of mrna.mrnas of primitive eukaryotes such as caenorhabditis elegans and ascaris summ possess two different caps at their 5' terminus. they have either a typical cap which consists of 7-methylguanosine linked via a 5',5'-triphosphate bridge to the first transcribed nucleotide (mmg cap) or an atypical hypermethylated form with two additional methyl groups at the n2 position (tmg cap). studies on interaction between the 5' end of mrna and proteins that specifically recognize its structure have been carrie ...201121701749
epidemiology of soil-transmitted helminthiases in malaysia.we reviewed the epidemiology of sth in malaysia from the 1970s to 2009. high prevalence rates persist among the rural aborigines, estate workers and in urban slums and squatter areas. trichuris trichiura is the most prevalent helminth in malaysia ranging from 2.1% to 98.2%. ascaris lumbricoides follows closely with a prevalence rate of 4.6-86.7%, while hookworm is the least prevalent (0-37.0%). a countrywide control program with special emphasis on school-based intervention is highly recommended ...201121706930
6questionnaire-based approach to assess schoolchildren's physical fitness and its potential role in exploring the putative impact of helminth and plasmodium spp. infections in c+¦te d'ivoire.abstract:201121702903
the polyprotein allergens of nematodes (npas) - structure at last, but still mysterious.we are engaged in structural and functional studies of several types of lipid binding protein that are only found in nematodes. amongst these are the nematode polyprotein allergens (npas) and we now report the solution structure of aba-1a (as-npa-a1), the most repeated unit within the npa array of ascaris suum, which is almost identical in amino acid sequence to that of ascaris lumbricoides. the protein forms a slightly flattened, compact, globular fold consisting of a long central helix that pa ...201121741973
correlation between malaria incidence and prevalence of soil-transmitted helminths in colombia: an ecologic evaluation.recent studies have suggested an association between the soil-transmitted helminth infections and malaria incidence. however, published evidence is still insufficient and diverging. since 1977, new ecologic studies have not been carried out to explore this association. ecologic studies could explore this correlation on a population level, assessing its potential importance on public health.201021713354
evidence for a peak shift in a humoral response to helminths: age profiles of ige in the shuar of ecuador, the tsimane of bolivia, and the u.s. nhanes.the peak shift model predicts that the age-profile of a pathogen's prevalence depends upon its transmission rate, peaking earlier in populations with higher transmission and declining as partial immunity is acquired. helminth infections are associated with increased immunoglobulin e (ige), which may convey partial immunity and influence the peak shift. although studies have noted peak shifts in helminths, corresponding peak shifts in total ige have not been investigated, nor has the age-patterni ...201121738813
ascaris lumbricoides causing infarction of the mesenteric lymph nodes and intestinal gangrene in a child: a case report.ascaris lumbricoides or round worm infestation is quite common in the developing world. it affects all age groups but is more common in children. most of the cases remain asymptomatic. the usual presentation is an intestinal obstruction. the physicians should be aware of this condition and consider it in the differential diagnosis when faced with such a case. the rare fatal complications include bleeding, perforation and gangrene.201121698086
geographical distribution of schistosomiasis and soil-transmitted helminths among school children in informal settlements in kisumu city, western kenya.summarythis cross-sectional study determined the prevalence and distribution of schistosome and soil-transmitted helminth (sth) infections among 1,308 children aged 10-18 years in 34 primary schools in 8 informal urban settlements in kisumu city, western kenya. stool samples were collected and examined for eggs of schistosoma mansoni and sth (hookworms, ascaris lumbricoides and trichuris trichiura) using the kato-katz technique. haematuria was used as a proxy indicator of urinary schistosomiasis ...201121679486
sedimentation of helminth eggs in water.helminth parasite eggs in low quality water represent health risks when used for irrigation of crops. the settling velocities of helminth eggs (ascaris suum, trichuris suis, and oesophagostomum spp.) and wastewater particles were experimentally determined in tap water and in wastewater using owen tubes. the settling velocities of eggs in tap water was compared with theoretical settling velocities calculated by stoke's law using measurements of size and density of eggs as well as density and visc ...201121741063
species-specific treatment effects of helminth/hiv-1 co-infection: a systematic review and meta-analysis.bacterial symbiosis and/or parasitism is widespread in hydrothermal bivalves, and is typically developed in gills, with a lower incidence in mantle and digestive glands, while it has never been described in byssus. using ultrastructural examination, we provide evidence for the existence of a potentially new group of filamentous prokaryotic organism in bathymodiolus azoricus byssus, with putative parasitic influence. additionally, a cystic, undefined organism was found with an unclear physiologic ...201121729353
risk factors associated with occurrence of nematodes in free range pigs in busia district, kenya.nematode infections are a serious constraint to pig production, especially where free range pig keeping is practiced. this study investigated the epidemiology of nematodes in free range pigs in busia district, kenya. three hundred and six pigs from 135 farms were sampled for faeces that were analysed for nematode eggs per gram (epg) of faeces using the mcmaster technique. the nematode eggs were also identified to genus and species based on morphology. a questionnaire on risk factors was also adm ...201121833678
a comparison of the sensitivity and fecal egg counts of the mcmaster egg counting and kato-katz thick smear methods for soil-transmitted helminths.the kato-katz thick smear (kato-katz) is the diagnostic method recommended for monitoring large-scale treatment programs implemented for the control of soil-transmitted helminths (sth) in public health, yet it is difficult to standardize. a promising alternative is the mcmaster egg counting method (mcmaster), commonly used in veterinary parasitology, but rarely so for the detection of sth in human stool.201121695104
prevention of soil-transmitted helminth infection.soil-transmitted helminths (sths) form one of the most important groups of infectious agents and are the cause of serious global health problems. the most important sths are roundworms (ascaris lumbricoides), whipworms (trichuris trichiura) and hookworms (necator americanus or ancylostoma duodenale); on a global level, more than a billion people have been infected by at least one species of this group of pathogens. this review explores the general concepts of transmission dynamics and the enviro ...201121731306
Intestinal Parasite Screening in Internationally Adopted Children: Importance of Multiple Stool Specimens.Objective: Our goal was to determine the prevalence of intestinal parasites in internationally adopted children, to examine factors associated with infection, and to determine if evaluating multiple stool specimens increases the yield of parasite identification. Methods: We evaluated internationally adopted children with at least 1 stool specimen submitted for ova and parasite testing within 120 days after arrival to the United States. In children submitting 3 stool specimens, in which at least ...201121824880
helminth parasites in pigs: new challenges in pig production and current research highlights.helminths in pigs have generally received little attention from veterinary parasitologists, despite ascaris suum, trichuris suis, and oesophagostomum sp. being common worldwide. the present paper presents challenges and current research highlights connected with these parasites. in danish swine herds, new indoor production systems may favour helminth transmission and growing knowledge on pasture survival and infectivity of a. suum and t. suis eggs indicates that they may constitute a serious thr ...201121684689
Comparison of the Kato-Katz and FLOTAC techniques for the diagnosis of soil-transmitted helminth infections.Decisions on individual or community treatment and evaluation of chemotherapy based control programs depend on parasitological diagnostic techniques. The aim of this study was to compare the accuracy of a single Kato-Katz thick smear and a single FLOTAC for the determination of the prevalence and intensity of soil-transmitted helminth infections. A total of 271 faecal specimens were collected from schoolchildren in Ethiopia, and microscopically examined using the Kato-Katz method (41.7mg stool p ...201121726662
deep small rna sequencing from the nematode ascaris reveals conservation, functional diversification, and novel developmental profiles.eukaryotic cells express several classes of small rnas that regulate gene expression and ensure genome maintenance. endogenous sirnas (endo-sirnas) and piwi-interacting rnas (pirnas) mainly control gene and transposon expression in the germline, while micrornas (mirnas) generally function in post-transcriptional gene silencing in both somatic and germline cells. to provide an evolutionary and developmental perspective on small rna pathways in nematodes, we identified and characterized known and ...201121685128
eosinophilic pneumonia due to visceral larva migrans possibly caused by ascaris suum: a case report and review of recent literatures.we report the case of a 62-year-old man who developed eosinophilic pneumonia due to visceral larva migrans (vlm) that was possibly caused by ascaris suum. the patient, a resident of the middle kyushu area who was found of eating raw porcine liver, complained of dry cough without dyspnea. the chest radiography showed a migration of infiltrative shadow. transbronchial lung biopsy of the right middle lobe revealed massive infiltration of eosinophils. the multi-dot enzyme-linked immunosorbent assay ...201121937827
[surveillance of soil-transmitted nematode infections in menghai county, yunnan province from 2006 to 2009].in the national monitoring point, menghai county, yunnan province, the total prevalence rate of soil-transmitted nematode infections was 88.12%, of which the rates of ascaris lumbricoides, trichuris trichiura, hookworm and cestode infections were 69.70%, 59.79%, 36.79%, and 1.30% respectively from 2006 to 2009. the infection intensities were slight. the ascaris lumbricoides infection rate in soil of vegetable garden was 44.5%. in conclusion, the infection rate of soil-transmitted nematodes is hi ...201122164489
Prevalence and risk factors of helminths and intestinal protozoa infections among children from primary schools in western Tajikistan.Intestinal parasitic infections represent a public health problem in Tajikistan, but epidemiological evidence is scarce. The present study aimed at assessing the extent of helminths and intestinal protozoa infections among children of 10 schools in four districts of Tajikistan, and to make recommendations for control.201121981979
soil-transmitted helminthiasis in the united states: a systematic review--1940-2010.the epidemiology of soil-transmitted helminth infections (hookworm, trichuris trichiura, ascaris lumbricoides, and strongyloides stercoralis) in the united states is poorly understood. to gain understanding of the status of disease, a systematic review was performed to assess the prevalence of soil-transmitted helminth infections in the united states. of all studies reviewed, 14 were designated as high-quality. high-quality studies were published from 1942 to 1982 and showed that infection was p ...201121976572
Evidence for phosphorylation in the MSP cytoskeletal filaments of amoeboid spermatozoa.Nematode spermatozoa are highly specialized cells that lack flagella and, instead, extend a pseudopod to initiate motility. Crawling spermatozoa display classic features of amoeboid motility (e.g. protrusion of a pseudopod that attaches to the substrate and the assembly and disassembly of cytoskeletal filaments involved in cell traction and locomotion), however, cytoskeletal dynamics in these cells are powered exclusively by Major Sperm Protein (MSP) rather than actin and no other molecular moto ...201122003439
changes in cyclic nucleotides, locomotory behavior, and body length produced by novel endogenous neuropeptides in the parasitic nematode ascaris suum.recent technical advances have rapidly advanced the discovery of novel peptides, as well as the transcripts that encode them, in the parasitic nematode ascaris suum. here we report that many of these novel peptides produce profound and varied effects on locomotory behavior and levels of cyclic nucleotides in a. suum. we investigated the effects of 31 endogenous neuropeptides encoded by transcripts afp-1, afp-2, afp-4, afp-6, afp-7, and afp-9-14 (afp: ascaris fmrfamide-like precursor protein) on ...201121854812
Necator americanus and helminth co-infections: further down-modulation of hookworm-specific type 1 immune responses.Helminth co-infection in humans is common in tropical regions of the world where transmission of soil-transmitted helminths such as Ascaris lumbricoides, Trichuris trichiura, and the hookworms Necator americanus and Ancylostoma duodenale as well as other helminths such as Schistosoma mansoni often occur simultaneously.201121909439
prevalence and types of coinfections in sleeping sickness patients in kenya (2000/2009).the occurrence of coinfections in human african trypanosomiasis (hat) patients was investigated using a retrospective data of hospital records at the national sleeping sickness referral hospital in alupe, kenya. a total of 31 patients, 19 males and 12 females, were diagnosed with hat between the years 2000 and 2009. the observed co-infections included malaria (100%), helminthosis (64.5%), typhoid (22.5%), urinary tract infections (16.1%), hiv (12.9%), and tuberculosis (3.2%). the species of helm ...201121915184
Current status of schistosomiasis and soil-transmitted helminthiasis in Beyla and Macenta Prefectures, Forest Guinea.A cross-sectional survey was undertaken in children aged 9-14 years in Beyla and Macenta Prefectures, Forest Guinea. Stool samples were examined by Kato-Katz and urine samples were examined by the centrifugation method. The overall prevalence and intensity of infection was 66.2% and 462.4 eggs per gram of faeces (epg) for Schistosoma mansoni, 21.0% and 17.8 eggs per 10ml of urine for S. haematobium, 51.2% and 507.5 epg for hookworm, 8.1% and 89.1 epg for Ascaris lumbricoides and 2.4% and 16.7 ep ...201121871646
how common is intestinal parasitism in hiv-infected patients in malaysia?human immunodeficiency virus (hiv)-infected individuals have greater susceptibility to infections by a myriad of microorganisms which can cause significant morbidity and mortality compared to immunocompetent individuals. of these microbial infections, intestinal parasitic infections (ipis) however are receiving less attention than bacterial and viral infections, hence, the lack of information of parasitic infections in hiv individuals. prevalence of ipis among 346 hiv-infected individuals in mal ...201122041762
[elimination of juvenile stages of ascaris lumbricoides (linnaeus, 1758) by mouth. report of a case and some epidemiological considerations].ascariosis is a parasitic disease caused by ascaris lumbricoides, a large geohelmint endemic in our country. at present, ascariosis is a rare infection in chile. we present a case of an adult, resident of the villa alemana municipality, valparaiso region, who spontaneously expelled two juvenile nematodes by mouth. we review the manifestations produced by the larval and adult stages of this parasite including their diagnosis, treatment, and epidemiological considerations.201122051626
distribution and characteristics of abfs, cecropins, nemapores, and lysozymes in nematodes.several groups of antimicrobial effector molecules have been identified in nematodes, but most studies have been limited to caenorhabditis elegans and, to a lesser extent, ascaris suum. although these two species are not closely related, they are not representative of overall nematode diversity. this study utilized available sequence information to investigate whether four groups of antimicrobial effectors (defensin-like antibacterial factors [abfs], cecropins, saposin domain-containing proteins ...201121978453
a truly emerging intestinal parasitosis. 201121896793
Ascaris lumbricoides-induced acute pancreatitis. 201222196821
disinfection of domestic effluents by gamma radiation: effects on the inactivation of ascaris lumbricoides eggs.this work investigated the inactivation of ascaris lumbricoides eggs in domestic effluents by gamma radiation from a (60)co source. domestic wastewater was treated in a compact demo-scale system consisting of a uasb reactor and a trickling filter; treatment was carried out at the center for research and training on sanitation (cepts), federal university of minas gerais, in belo horizonte-mg, brazil. one-liter of treated wastewater samples was artificially contaminated with an average of 1000 non ...201121911240
the effect of single and multiple infections on atopy and wheezing in children.background: the current epidemic of asthma and atopy has been explained by alterations in immune responses related to reduction in childhood infections. however, the findings of epidemiologic studies investigating the association between infection with atopy and asthma have been inconsistent. objective: we sought to investigate the effect of single or multiple infections (pathogen burden) on atopy and wheeze in urban children from latin america. methods: specific ige against aeroallergens (sige) ...201122035877
developmentally programmed, rna-directed genome rearrangement in tetrahymena.developmentally programmed genome rearrangement has been observed in a variety of eukaryotes from vertebrates to worms to protists, and it provides an interesting exception to the general rule of the constancy of the genome. dna elimination in the ciliated protozoan tetrahymena is one of the most well-characterized programmed genome rearrangement events. dna elimination in the newly formed macronucleus of tetrahymena is epigenetically regulated by the dna sequence of the parental macronucleus. d ...201122103557
characterization of baylisascaris schroederi from qinling subspecies of giant panda in china by the first internal transcribed spacer (its-1) of nuclear ribosomal dna.in the present study, a total of 20 nematode isolates, (including 10 male and 10 female worms) representing baylisascaris schroederi from 5 qinling subspecies of giant pandas (ailuropoda melanoleuca) in shaanxi province of china, were characterized and grouped genetically by the first internal transcribed spacer (its-1) of nuclear ribosomal dna (rdna). the rdna fragment spanning 3' end of 18s rdna, complete its-1 rdna, and 5' end of 5.8s rdna were amplified and sequenced. the sequence variabilit ...201121870244
A novel C-type lectin identified by EST analysis in tissue migratory larvae of Ascaris suum.C-type lectins (CTLs) are a group of proteins which bind to carbohydrate epitopes in the presence of Ca(2+), which have been described in a wide range of species. In this study, a cDNA sequence coding a putative CTL has been identified from the cDNA library constructed from the pig round worm Ascaris suum lung L3 (LL3) larvae, which was designated as A. suum C-type lectin-1 (As-CTL-1). The 510 nucleotide open reading frame of As-CTL-1 cDNA encoded the predicted 169 amino acid protein including a ...201122006188
Coprological survey of alimentary tract parasites in dogs from Zambia and evaluation of a coproantigen assay for canine echinococcosis.Faecal samples were collected from the rectum of 540 domestic dogs from four districts (Lusaka, Katete, Petauke and Luangwa) in Zambia between 2005 and 2006 and prevalences of canine alimentary tract parasites were determined by coprological examination. Thirteen different ova and parasites including strongyle (43·3%), Spirocerca lupi (18·7%), taeniid (13·1%), Toxocara canis (7·6%), Sarcocystis sp.* (7·5%), Isospora sp.* (5·7%), Physaloptera sp.* (4·6%), Capillaria sp.* (2·8%), Dipylidium cani ...201122185947
epidemiological and clinical picture of parasitic infections in the group of children and adolescents from north-east region of poland.the epidemiological and clinical purview of parasite diseases is constantly evolving, mainly due to the changes in human behavior and our environment. the aim of this study was frequency analysis of certain parasite infections, risk factors and clinical symptoms in children and adolescents of north-eastern poland. 120 children were tested due to the symptoms resembling parasite infection from december 2008 to may 2009. 90 patients (the tested group) aged from 5 months to 18 years were found to b ...201122165741
soil-transmitted helminth infections among plantation sector schoolchildren in sri lanka: prevalence after ten years of preventive chemotherapy.the plantation sector in sri lanka lags behind the rest of the country in terms of living conditions and health. in 1992, a sector-wide survey of children aged 3-12 years and women of reproductive age showed >90% prevalence of soil-transmitted helminth infections. biannual mass de-worming targeting children aged 3-18 years started in 1994 and was continued until 2005. the present study was carried out to assess the status of infection four years after cessation of mass de-worming.201121980549
environmental analyses of the parasitic profile found in the sandy soil from the santos municipality beaches, sp, brazil.the environmental contamination by geohelminths represents a world public health problem and has been well documented by several authors. however, few papers describe the presence of such contamination in saline soils of coastal beaches. a study was performed on the beaches of the municipality of santos in the period between may 2004 to april 2005 with the aim of determining the degree of contamination, and the correlation between contamination level and seasonal conditions and characteristics o ...201122012454
interactions between worms and malaria: good worms or bad worms?in the past decade there have been an increasing number of studies on co-infections between worms and malaria. however, this increased interest has yielded results that have been at times conflicting and made it difficult to clearly grasp the outcome of this interaction. despite the heterogeneity of study designs, reviewing the growing body of research may be synthesized into some broad trends: ascaris emerges mostly as protective from malaria and its severe manifestations, whereas hookworm seem ...201121910854
[sensitization to ascaris lumbricoides and asthma severity in children].there is little information concerning asthma severity and sensitization to ascaris lumbricoides in pediatric patients.201121870728
prevalence of intestinal parasites among pupils in rural north eastern, nigeria.background: the study determined the prevalence of intestinal parasitism among pupils in rural schools (almajiris) in konduga local government area of borno state. materials and methods: a total of 257 stool specimens were collected at random among pupils (almajiris) in rural quranic schools; the stools were processed and examined both macroscopically and microscopically by concentration techniques. results: the prevalence of intestinal parasitism among the almajiris was 80.9%. the highest pre ...201121969128
[longitudinal investigation on intestinal parasite infections among rural people in west dongting lake region].the nylon pocket concentration method and modified kato-katz technique were used to detect the eggs of intestinal parasites and the iodine smear method was used for the detection of protozoa among the rural population in west dongting lake region. the infection rate of parasites in 2006 was 11.84%, and it declined by 86.63%, 81.34%, and 47.28%, respectively, compared to the rates in 1983, 1993, and 2003. six major parasites were detected including ascaris lumbricoides, trichuris trichiura, fasci ...201122164496
blastocystis hominis as a contributing risk factor for development of iron deficiency anemia in pregnant women.intestinal parasitic infection increases the risk of developing iron deficiency anemia (ida) during pregnancy. the objective of this study was to assess blastocystis hominis as a contributing risk factor for development of ida in pregnant women. a total of 200 fecal specimens from 120 pregnant women with ida (mean hb = 9.6 g/dl), and 80 non-anemic controls were examined for blastocystis. fecal specimens were examined by the formalin/ethyl-acetate concentration technique, iron hematoxylin stainin ...201122167376
evolution of embryonic development in nematodes.abstract:201121929824
ascaris lumbricoides as etiologic factor for pancreas inflammatory tumor. 201122149563
the influence of different helminth infection phenotypes on immune responses against hiv in co-infected adults in south africa.the convergent distribution of the human immunodeficiency virus (hiv) and helminth infections has led to the suggestion that infection with helminths exacerbates the hiv epidemic in developing countries. in south africa, it is estimated that 57% of the population lives in poverty and carries the highest burden of both hiv and helmith infections, however, the disease interactions are under-researched.201121999928
picture of the month. recurrent biliary colic in a young girl. 201121969397
a prospective study on parasites among expatriate workers in al-baha from 2009-2011, saudi arabia.al-baha is an area with large influx of expatriate workers in saudi arabia. this study was carried out from october 2009 to january 2011 on expatriate workers (2000) to determine the prevalence of parasitosis. urine and stool samples were collected and specifically examined. the results showed that 1079 (53.95%) were infected with one or up to ten parasitic species. trichuris trichiura had the highest prevalence 190 (17.6%). other helminthes were ascaris lumbricoides 179 (16.58%), ancylostoma du ...201121980781
intervention to prevent intestinal parasitic reinfections among tarahumara indigenous schoolchildren in northern mexico.to assess the effectiveness of a 20-week, broad intervention to prevent reinfection by ascaris lumbricoides (al) and giardia lamblia (gl) among indigenous schoolchildren in northern mexico.201122069065
the complete mitochondrial genome of human parasitic roundworm, ascaris lumbricoides.the genome length of the ascaris lumbricoides, human parasitic roundworm, is 14,281 bp with a nucleotide composition of 22.1% a, 49.8% t, 7.8% c, and 20.3% g. the genome consists of 12 protein-coding genes, 2 rrna genes, 22 trna genes, and 1 control region.201122040074
molecular insights for giardia, cryptosporidium, and soil-transmitted helminths from a facility-based surveillance system in guatemala.abstract. we molecularly characterized samples with giardia, cryptosporidium, and soil-transmitted helminths from a facility-based surveillance system for diarrhea in santa rosa, guatemala. the dna sequence analysis determined the presence of giardia assemblages a (n = 7) and b (n = 12) and, cryptosporidium hominis (n = 2) and cryptosporidium parvum (n = 2), suggestive of different transmission cycles. all 41 samples with soil-transmitted helminths did not have the β-tubulin mutation described f ...201122144459
reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction.crawling movement in eukaryotic cells requires coordination of leading-edge protrusion with cell body retraction [1-3]. protrusion is driven by actin polymerization along the leading edge [4]. the mechanism of retraction is less clear; myosin contractility may be involved in some cells [5] but is not essential in others [6-9]. in ascaris sperm, protrusion and retraction are powered by the major sperm protein (msp) motility system instead of the conventional actin apparatus [10, 11]. these cells ...201122000106
actin depolymerization-based force retracts the cell rear in polarizing and migrating cells.in migrating cells, the relative importance of myosin ii contractility for cell rear retraction varies [1-12]. however, in myosin ii-inhibited polarizing cells, actin organization is compromised [13-18]; thus it remains unclear whether myosin ii is simply required for correct actin arrangement or also directly drives rear retraction [9]. ascaris sperm cells lack actin and associated motors, and depolymerization of major sperm protein is instead thought to pull the cell rear forward [19, 20]. opp ...201122137472
intestinal parasitosis and shigellosis among diarrheal patients in gondar teaching hospital, northwest ethiopia.abstract:201122041102
asymptomatic falciparum malaria and intestinal helminths co-infection among school children in osogbo, nigeria.malaria and intestinal helminths are parasitic diseases causing high morbidity and mortality in most tropical parts of the world, where climatic conditions and sanitation practices favor their prevalence. the aim of this study was to determine the prevalence and possible impact of falciparum malaria and intestinal helminths co-infection among school children in kajola, osun state, nigeria.201122091292
Determinants of nucleotide-binding selectivity of malic enzyme.Malic enzymes have high cofactor selectivity. An isoform-specific distribution of residues 314, 346, 347 and 362 implies that they may play key roles in determining the cofactor specificity. Currently, Glu314, Ser346, Lys347 and Lys362 in human c-NADP-ME were changed to the corresponding residues of human m-NAD(P)-ME (Glu, Lys, Tyr and Gln, respectively) or Ascaris suum m-NAD-ME (Ala, Ile, Asp and His, respectively). Kinetic data demonstrated that the S346K/K347Y/K362Q c-NADP-ME was transformed ...201121980421
nucleic acid transfection and transgenesis in parasitic nematodes.summarytransgenesis is an essential tool for assessing gene function in any organism, and it is especially crucial for parasitic nematodes given the dwindling armamentarium of effective anthelmintics and the consequent need to validate essential molecular targets for new drugs and vaccines. two of the major routes of gene delivery evaluated to date in parasitic nematodes, bombardment with dna-coated microparticles and intragonadal microinjection of dna constructs, draw upon experience with the f ...201121880161
prevalence and epidemiology of intestinal parasitism, as revealed by three distinct techniques in an endemic area in the brazilian amazon.this survey aims to estimate the prevalence of intestinal parasitic infections in santa isabel do rio negro, amazonian brazil, through three distinct techniques, correlating the prevalence rates with family income and age groups as well as assessing the household clustering of infections. prevalence rates were assessed through graham (n=113), baermann-moraes (n=232) and ritchie (n=463) methods. the graham method was adopted only for children under 5 years old, 15% of whom were positive for enter ...201122117850
intestinal volvulus: aetiology, morbidity and mortality in tunisian children.intestinal volvulus (iv) can occur at various sites of the gastrointestinal tract. in europe, iv in children is most frequently due to malrotation but in asia ascaris infestation is a common cause. this report reviews the experience with iv in children in tunisia; analyzes the aetiologies as well as the clinical presentations and the benefits of the ladd's procedure in the treatment of the iv.201122005353
ascaris suum draft genome.parasitic diseases have a devastating, long-term impact on human health, welfare and food production worldwide. more than two billion people are infected with geohelminths, including the roundworms ascaris (common roundworm), necator and ancylostoma (hookworms), and trichuris (whipworm), mainly in developing or impoverished nations of asia, africa and latin america. in humans, the diseases caused by these parasites result in about 135,000 deaths annually, with a global burden comparable with tha ...201122031327
Salmeterol or doxycycline do not inhibit acute bronchospasm and airway inflammation in cats with experimentally-induced asthma.The objective of this study was to determine if inhaled salmeterol, a long-acting ß(2)-adrenergic agonist, and oral doxycycline, a tetracycline antibiotic displaying matrix metalloproteinase (MMP) inhibitory activity, reduce airway inflammation and obstruction in cats with experimentally-induced asthma. Eight Ascaris suum (AS)-sensitised cats were enrolled in a prospective study in which they underwent four AS-challenges at 1month intervals. The challenged animals were given no treatment or were ...201122136877
national monitoring of ascaris suum related liver pathologies in english abattoirs: a time-series analysis, 2005-2010.ascaris suum is the most important internal parasite in farmed pigs world-wide. in england, the bpex pig health scheme (bphs) monitors the prevalence of ascariosis in slaughtered finished pigs by identifying milk spots - the healing lesions caused by a. suum larvae migration through the liver. this study investigates the trend of milk spot lesions from july 2005 to december 2010 to identify the progress made by the industry in controlling this parasitic disease. for visual explorations, the mont ...201121889266
Endoscopic Diagnosis in Ascaris lumbricoides Case with Pyloric Obstruction.Ascaris lumbricoides is the largest and most prevalent helminth seen in the human body. Ascariasis having high morbidity and mortality causes a unique type of intestinal obstruction with specific problems. This is probably due to reduced intestinal absorption and luminal obstruction, which can lead to anorexia and blockage of the absorbing surface. It affects humans especially in developing countries. This essay presented a 78- year- old female case had severe abdominal p ...201122198921
structural basis for nematode eif4e binding an m(2,2,7)g-cap and its implications for translation initiation.metazoan spliced leader (sl) trans-splicing generates mrnas with an m(2,2,7)g-cap and a common downstream sl rna sequence. the mechanism for eif4e binding an m²²⁷g-cap is unknown. here, we describe the first structure of an eif4e with an m(2,2,7)g-cap and compare it to the cognate m⁷g-eif4e complex. these structures and nuclear magnetic resonance (nmr) data indicate that the nematode ascaris suum eif4e binds the two different caps in a similar manner except for the loss of a single hydrogen bond ...201121965542
Serological diagnosis of clonorchiasis: using a recombinant propeptide of cathepsin L proteinase from Clonorchis sinensis as a candidate antigen.Clonorchiasis is a common zoonosis in southern and northeastern parts of China, especially in Guangdong, Guangxi and Jilin province. Anti-Clonorchis sinensis antibody detection by enzyme-linked immunosorbent assay (ELISA) has been used for epidemiological surveys of clonorchiasis for its convenience and celerity, but it is still a meaningful work to screen ideal diagnostic antigen or antibody subtype for improvement of diagnostic sensitivity and specificity and for judgement of curative effect. ...201122173451
management of biliary ascaris lumbricoides in kabul, afghanistan: crossroads of advancing technology.the case is presented of the successful removal of a dead ascaris lumbricoides from the right hepatic duct of a middle aged woman from a rural province in afghanistan. the case was started laparoscopically, but converted to an open procedure because of difficulty identifying the anatomic landmarks required to safely perform laparoscopic cholecystectomy. after worm removal, the common bile duct (cbd) was reconstructed with a roux-en-y hepaticojejunostomy. the patient was discharged on postoperati ...200922171238
parasitic infections, anemia and malnutrition among rural settled and mobile pastoralist mothers and their children in chad.malnutrition, resulting from various etiologies, is common in rural chadian women and children. this cross-sectional study assessed the spectrum of parasitic infection and level of anemia and their effect on nutritional status in settled and mobile pastoral mothers and children near lake chad. intestinal parasites were evaluated using direct fecal smears and the kato-katz technique. malaria status was determined using paracheck-pf(®) rapid diagnostic test, and anemia was assessed with the hemocu ...201122160444
genetic diversity of ascaris in southwestern uganda.despite the common occurrence of ascariasis in southwestern uganda, helminth control in the region has been limited. to gain further insights into the genetic diversity of ascaris in this area, a parasitological survey in mothers (n=41) and children (n=74) living in two villages, habutobere and musezero, was carried out. adult ascaris worms were collected from infected individuals by chemo-expulsion using pyrantel pamoate treatment. genetic diversity within these worms was assessed by inspection ...201122192492
Adult ascaris worm passing from the mouth. 201121896792
risk factors for helminth infections in a rural and a peri-urban setting of the dongting lake area, people's republic of china.schistosomiasis japonica and soil-transmitted helminthiasis are endemic parasitic diseases in the people's republic of china (pr china). as very few studies have reported on the distribution and interaction of multiple species helminth infections, we carried out a comparative study of households in a rural village and a peri-urban setting in the dongting lake area of hunan province in november and december 2006 to determine the extent of single and multiple species infections, the underlying ris ...201121854780
Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad.Ascaris suum (large roundworm of pigs) is a parasitic nematode that causes substantial losses to the meat industry. This nematode is suitable for biochemical studies because, unlike C. elegans, homogeneous tissue samples can be obtained by dissection. It has large sperm, produced in great numbers that permit biochemical studies of sperm motility. Widespread study of A. suum would be facilitated by more comprehensive genome resources and, to this end, we have produced a gonad transcriptome of A. ...201121962222
the impact of health education on the prevalence of faecal-orally transmitted parasitic infections among school children in a rural community in cameroon.faecal-orally transmitted parasites are those parasites which are spread through faecal contamination of food and drinks. infections with these parasites are among the most common in the world being responsible for considerable morbidity and mortality, especially in children. this study was carried out to determine the impact of health education on the prevalence of faecal-orally transmitted parasitic infections among primary school children in a typical african rural community.201122121446
Radio-pathological diagnosis of hepatobiliary ascariasis: A rare entity.Ascariasis is the commonest helminthic infection in humans, caused by the nematode Ascaris lumbricoides. The adult worms usually reside in the small intestine. Rarely, they migrate into the bile duct and pancreatic ducts, but involvement of the gall bladder and/or liver parenchyma is extremely rare. Here, we describe a case of a 32-year-old woman who presented with fever, anorexia, right upper quadrant pain and mild hepatomegaly. Ultrasonography revealed a liver abscess and a concurrent moving a ...201121897545
Efficacy of single-dose and triple-dose albendazole and mebendazole against soil-transmitted helminths and Taenia spp.: a randomized controlled trial.The control of soil-transmitted helminth (STH) infections currently relies on the large-scale administration of single-dose oral albendazole or mebendazole. However, these treatment regimens have limited efficacy against hookworm and Trichuris trichiura in terms of cure rates (CR), whereas fecal egg reduction rates (ERR) are generally high for all common STH species. We compared the efficacy of single-dose versus triple-dose treatment against hookworm and other STHs in a community-based randomiz ...201121980373
comparative analyses of the complete mitochondrial genomes of ascaris lumbricoides and ascaris suum from humans and pigs.ascaris lumbricoides and ascaris suum are parasitic nematodes living in the small intestine of humans and pigs, and can cause the disease ascariasis. for long, there has been controversy as to whether the two ascaridoid taxa represent the same species due to their significant resemblances in morphology. however, the complete mitochondrial (mt) genome data have been lacking for a. lumbricoides in spite of human and animal health significance and socio-economic impact globally of these parasites. ...201222075400
[two surveys of human important parasitic diseases in xihu district, hangzhou city].to understand the status and epidemic rules of human important parasite infections, and evaluate the control effect in xihu district, so as to provide evidence for improving future parasitic disease control.201122164383
[evaluation on intervention measures of comprehensive control for parasitic diseases in demonstration plot of xiangyun county].to evaluate the effect of comprehensive intervention measures to control and prevent parasitic diseases in the demonstration plot of xiangyun county, so as to provide the evidence for establishing appropriate measures of parasitic diseases control and prevention.201122263501
malnutrition and intestinal helminth infections in schoolchildren from dibanda, cameroon.intestinal helminth infections and malnutrition constitute a major health burden in developing countries, with infants and children being the most vulnerable groups. the extent of the burden of intestinal helminth infections and malnutrition was investigated in a cross-sectional study involving 265 children aged between 4 and 14 years residing in dibanda, a semi-rural area located in buea, south west region, cameroon. the prevalence of intestinal helminth infections was determined microscopicall ...201222273401
mitochondrial fumarate reductase as a target of chemotherapy: from parasites to cancer cells.recent research on respiratory chain of the parasitic helminth, ascaris suum has shown that the mitochondrial nadh-fumarate reductase system (fumarate respiration), which is composed of complex i (nadh-rhodoquinone reductase), rhodoquinone and complex ii (rhodoquinol-fumarate reductase) plays an important role in the anaerobic energy metabolism of adult parasites inhabiting hosts. the enzymes in these parasite-specific pathways are potential target for chemotherapy. we isolated a novel compound, ...201122226661
[effect of comprehensive control on parasitic diseases in demonstration plot of hainan province].to evaluate the effect of control on soil-transmitted nematode infections in the demonstration plot of integrated control on parasitic diseases, hainan province.201122263512
in vitro and in vivo efficacy of monepantel (aad 1566) against laboratory models of human intestinal nematode infections.few effective drugs are available for soil-transmitted helminthiases and drug resistance is of concern. in the present work, we tested the efficacy of the veterinary drug monepantel, a potential drug development candidate compared to standard drugs in vitro and in parasite-rodent models of relevance to human soil-transmitted helminthiases.201122216366
identification of trans-4-[1-[[7-fluoro-2-(1-methyl-3-indolyl)-6-benzoxazolyl]acetyl]-(4s)-fluoro-(2s)-pyrrolidinylmethoxy]cyclohexanecarboxylic acid as a potent, orally active vla-4 antagonist.for the purpose of obtaining orally potent vla-4 inhibitors, we have carried out structural modification of the (n'-phenylureido)phenyl group in compound 1, where the group was found to be attributed to poor pharmacokinetic profile in our previous research. through modification, we have identified several compounds with both potent in vitro activity and improved oral exposure. in particular, compound 7e with 7-fluoro-2-(1-methyl-1h-indol-3-yl)-1,3-benzoxazolyl group as a novel replacement of the ...201122261021
trends and prevalence of intestinal parasites at a tertiary care center in lebanon over a decade.intestinal parasitic infections or infestation are amongst the most prevalent infections worldwide. this study aimed at revealing the changing trends over a decade duration of intestinal parasites identified at a major tertiary care center in lebanon between 1997-1998 and 2007-2008. the total number of specimens tested were 14,771 for 1997-1998 vs 7477 for 2007-2008. the positive findings for parasites were 2077 (14%) vs 1047 (14%), respectively. the majority of recovered parasites in both study ...201122259902
[prevention and treatment of ascariasis in demonstration plots of integrated control from 2006 to 2009].to understand the situation of ascaris lumbricoides infection in the demonstration plots and evaluate the effect of integrated intervention measures.201122263492
[effect of comprehensive control in demonstration plots of parasitic diseases in guizhou province].to evaluate the effect of comprehensive control in demonstration plots of parasitic diseases in guizhou province.201122263498
a parasitological survey of natural water springs and inhabitants of a tourist city in southeastern brazil.abstract the goals of this study were to investigate the occurrence of cryptosporidium oocysts and giardia cysts in main springs of campos do jordão, an important tourist city, in brazil and to gather the largest amount of parasitological data from autochthonous population that live in rural areas of this city. the membrane filtration technique followed by direct immunofluorescence assay was employed for concentration and visualization of waterborne protozoa. in the period between june 2003 an ...201222217166
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