| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| surface modification of a polyethylene film for anticoagulant and anti-microbial catheter. | a functional anticoagulant and anti-bacterial coating for polyethylene (pe) films is described. the stepwise preparation of this nanocomposite surface coating involves o2 plasma etching of pe film, carbodiimide coupling of cysteamine to the etched pe film, binding of ag to sulfhydryl groups of cysteamine, and assembly of heparin capped agnps on the pe film. the nanocomposite film and its components were characterized by (1)h-nuclear magnetic resonance spectroscopy, attenuated total reflectance-f ... | 2016 | 26900340 |
| fabrication of an electrochemical dna-based biosensor for bacillus cereus detection in milk and infant formula. | this paper describes fabrication of a dna-based au-nanoparticle modified pencil graphite electrode (pge) biosensor for detection of bacillus cereus, causative agent of two types of food-borne disease, i.e., emetic and diarrheal syndrome. the sensing element of the biosensor was comprised of gold nanoparticles (gnps) self-assembled with single-stranded dna (ssdna) of nhea gene immobilized with thiol linker on the gnps modified pge. the size, shape and dispersion of the gnps were characterized by ... | 2016 | 26896793 |
| bacillus licheniformis trehalose-6-phosphate hydrolase structures suggest keys to substrate specificity. | trehalose-6-phosphate hydrolase (trea) belongs to glycoside hydrolase family 13 (gh13) and catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to yield glucose and glucose 6-phosphate. the products of this reaction can be further metabolized by the energy-generating glycolytic pathway. here, crystal structures of bacillus licheniformis trea (bltrea) and its r201q mutant complexed with p-nitrophenyl-α-d-glucopyranoside (r201q-ppng) are presented at 2.0 and 2.05 å resolution, respectively. the ... | 2016 | 26894535 |
| bacteria-based polythene degradation products: gc-ms analysis and toxicity testing. | polythene degradation leads to the production of various by-products depending upon the type of degradation process. the polythene degradation products (pedp) in the culture supernatant of the two bacteria (lysinibacillus fusiformis strain vasb14/wl and bacillus cereus strain vasb1/ts) were analyzed with gc-ms technique. the major by-products in the pedp in the culture supernatant of l. fusiformis strain vasb14/wl (1,2,3,4 tetra methyl benzene) and b. cereus strain vasb1/ts (1,2,3 trimethyl benz ... | 2016 | 26888528 |
| association of genotyping of bacillus cereus with clinical features of post-traumatic endophthalmitis. | bacillus cereus is the second most frequent cause of post-traumatic bacterial endophthalmitis. although genotyping of b. cereus associated with gastrointestinal infections has been reported, little is known about the b. cereus clinical isolates associated with post-traumatic endophthalmitis. this is largely due to the limited number of clinical strains available isolated from infected tissues of patients with post-traumatic endophthalmitis. in this study, we report successful isolation of twenty ... | 2016 | 26886446 |
| investigation of equilibrium and kinetics of cr(vi) adsorption by dried bacillus cereus using response surface methodology. | in this study, response surface methodology (rsm) based on three-variable-five-level central composite rotatable design was used to analyze the effects of combined and individual operating parameters (biomass dose, initial concentration of cr(vi) and ph) on the cr(vi) adsorption capacity of dried bacillus cereus. a quadratic polynomial equation was obtained to predict the adsorbed cr(vi) amount. analysis of variance showed that the effect of biomass dose was the key factor in the removal of cr(v ... | 2016 | 26877045 |
| the pulsed light inactivation of veterinary relevant microbial biofilms and the use of a rtpcr assay to detect parasite species within biofilm structures. | the presence of pathogenic organisms namely parasite species and bacteria in biofilms in veterinary settings, is a public health concern in relation to human and animal exposure. veterinary clinics represent a significant risk factor for the transfer of pathogens from housed animals to humans, especially in cases of wound infection and the shedding of faecal matter. this study aims to provide a means of detecting veterinary relevant parasite species in bacterial biofilms, and to provide a means ... | 2016 | 26862516 |
| uptake and levels of the antibiotic berberine in individual dormant and germinating clostridium difficile and bacillus cereus spores as measured by laser tweezers raman spectroscopy. | spores of clostridium difficile and bacillus cereus are major causes of nosocomial diarrhoea and foodborne disease. our aim was to measure the dynamics of the uptake of the antibiotic berberine by individual germinating spores and the levels of berberine accumulated in germinated spores. | 2016 | 26861569 |
| antioxidant and dna damage protecting activity of exopolysaccharides from the endophytic bacterium bacillus cereus sz1. | an endophytic bacterium was isolated from the chinese medicinal plant artemisia annua l. the phylogenetic and physiological characterization indicated that the isolate, strain sz-1, was bacillus cereus. the endophyte could produce an exopolysaccharide (eps) at 46 mg/l. the 1,1-diphenyl-2-picrylhydracyl (dpph) radical scavenging activity of the eps reached more than 50% at 3-5 mg/ml. the eps was also effective in scavenging superoxide radical in a concentration dependent fashion with an ec50 valu ... | 2016 | 26861269 |
| understanding the antimicrobial activity behind thin- and thick-rolled copper plates. | the aim of this study was to compare the antibacterial properties of the surfaces of copper plates that were rolled to a thickness of 25 and 100 μm. differences in topology of 25- and 100-μm-thick copper plates were studied using scanning electron microscopy (sem), atomic force microscopy (afm), and x-ray diffraction (xrd). antibacterial activity of the copper surfaces was tested against strains of staphylococcus aureus, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, listeria m ... | 2016 | 26860943 |
| biological production of silver nanoparticles by soil isolated bacteria and preliminary study of their cytotoxicity and cutaneous wound healing efficiency in rat. | biosynthesis of agnps by 37 different bacterial soil isolates was done and confirmed through visible spectrophotometry. fifteen isolates were identified and two of them with the highest ability of agnps production were used for transmission electron microscopy (tem) and x-ray diffraction (xrd) tests. mtt assay for both of the obtained agnps was run and after determination their ic50s, two different toxic and nontoxic doses of each agnps solution were chosen for wound healing assay. forty eight r ... | 2016 | 26854241 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| detection and quantification of viable bacillus cereus group species in milk by propidium monoazide quantitative real-time pcr. | the bacillus cereus group includes important spore-forming bacteria that present spoilage capability and may cause foodborne diseases. these microorganisms are traditionally evaluated in food using culturing methods, which can be laborious and time-consuming, and may also fail to detect bacteria in a viable but nonculturable state. the purpose of this study was to develop a quantitative real-time pcr (qpcr) combined with a propidium monoazide (pma) treatment to analyze the contamination of uht m ... | 2016 | 26830746 |
| antagonistics against pathogenic bacillus cereus in milk fermentation by lactobacillus plantarum zdy2013 and its anti-adhesion effect on caco-2 cells against pathogens. | lactobacillus plantarum zdy2013 is a potential probiotic isolated from fermented bean acid. in this study, we aimed to evaluate the in vitro antimicrobial activity of this organism against bacillus cereus in milk fermentation, the antiadhesion ability on intestinal epithelial cells, as well as its ability to abrogate the cytotoxic effect and expression levels of genes. we found no antimicrobial activity produced by l. plantarum once the ph was adjusted to 6.0 and 7.0. the ph decreased continuous ... | 2016 | 26830743 |
| isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris). | shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ... | 2016 | 26822957 |
| antibacterial and synergistic activity of pentacyclic triterpenoids isolated from alstonia scholaris. | (1) background: alstonia scholaris (apocynaceae) is an important medicinal plant that has been historically used in "dai" ethnopharmacy to treat infectious diseases in china. although various pharmacological activities have been reported, the antimicrobial constitutes of a. scholaris have not yet been identified. the objective of this study is to evaluate the antibacterial constitutes from the leaf extract of a. scholaris and to assess the synergistic effects of isolated compounds with antibioti ... | 2016 | 26821000 |
| in vitro antibacterial activity of selected medicinal plants traditionally used in vietnam against human pathogenic bacteria. | medicinal plants are widely used for the treatment of different infectious diseases. infectious diseases caused by bacteria have a large impact on public health. this study aimed to determine the in vitro antibacterial activity of the medicinal plants traditionally used in vietnam against the bacterial strains associated with infectious diseases. | 2016 | 26819218 |
| characterization and exposure assessment of emetic bacillus cereus and cereulide production in food products on the dutch market. | the emetic toxin cereulide, which can be produced by bacillus cereus, can be the cause of food poisoning upon ingestion by the consumer. the toxin causes vomiting and is mainly produced in farinaceous food products. this article includes the prevalence of b. cereus and of cereulide in food products in the netherlands, a characterization of b. cereus isolates obtained, cereulide production conditions, and a comparison of consumer exposure estimates with those of a previous exposure assessment. fo ... | 2016 | 26818983 |
| antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.). | an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ... | 2016 | 26809052 |
| evaluation of probiotic properties of lactobacillus plantarum wlpl04 isolated from human breast milk. | lactobacillus plantarum wlpl04, a specific strain isolated from human breast milk, was investigated for its survival capacity (acid and bile salt tolerance, survival in simulated gastrointestinal tract, inhibition of pathogens, antibiotic susceptibility, yield of exopolysaccharides) and probiotic properties (antiadhesion of pathogens, protection from harmful effect of sodium dodecyl sulfate, and antiinflammatory stress on caco-2 cells). the results showed that lb. plantarum wlpl04 had broad-spec ... | 2016 | 26805974 |
| characterization of antimicrobial lipopeptides produced by bacillus sp. lm7 isolated from chungkookjang, a korean traditional fermented soybean food. | a wild-type microorganism exhibiting antimicrobial activities was isolated from the korean traditional fermented soybean food chungkookjang and identified as bacillus sp. lm7. during its stationary growth phase, the microorganism secreted an antimicrobial substance, which we partially purified using a simple two-step procedure involving ammonium sulfate precipitation and heat treatment. the partially purified antimicrobial substance, anti-lm7, was stable over a broad ph range (4.0-9.0) and at te ... | 2016 | 26803269 |
| diversity of sugar acceptor of glycosyltransferase 1 from bacillus cereus and its application for glucoside synthesis. | glycosyltransferase 1 from bacillus cereus (bcgt1) catalyzes the transfer of a glucosyl moiety from uridine diphosphate glucose (udp-glucose) to various acceptors; it was expressed and characterized. the specificity of acceptors was found to be broad: more than 20 compounds classified into o-, s-, and n-linkage glucosides can be prepared with bcgt1 catalysis. based on this work, we conclude that the corresponding acceptors of these compounds must possess the following features: (1) the acceptors ... | 2016 | 26795959 |
| α,β-dibromochalcone derivatives--synthesis and antimicrobial activity. | to obtain some chalcones and their dibrominated analogues and to evaluate their antimicrobial potential. | 2016 | 26793867 |
| infective endocarditis due to bacillus cereus in a pregnant female: a case report and literature review. | incidence of infective endocarditis during pregnancy is around 0.006% with high maternal and fetal mortality. bacillus cereus is an extremely rare cause for endocarditis in intravenous drug abusers (ivda) or those with valvular disease or devices such as pacemakers. we report a case of b. cereus endocarditis, which, to the best of our knowledge, has never been reported in pregnancy. a 30-year-old, 25-week pregnant female presented with right shoulder pain, swelling and erythema on the lateral as ... | 2015 | 26793477 |
| isolation, purification and characterization of antimicrobial protein from seedlings of bauhinia purpurea l. | a novel antimicrobial protein was purified from the seedlings of bauhinia purpurea by sequential procedures entailing ammonium sulfate precipitation, cation exchange chromatography, preparative native-page and a yield of 2.7% was obtained from the crude extract. the purified antimicrobial protein appeared as a single protein band on sds-page with the molecular mass of 20.9 kda. purified antimicrobial protein exhibited a potent antimicrobial activity against both gram-positive and gram-negative b ... | 2016 | 26780468 |
| cody regulates the activity of the virulence quorum sensor plcr by controlling the import of the signaling peptide papr in bacillus thuringiensis. | in gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors of the rnpp family. activity of these regulators depends on their binding to secreted signaling peptides that are imported into the cell. these quorum sensing regulators control important biological functions in bacteria of the bacillus cereus group, such as virulence and necrotrophism. the rnpp quorum sensor plcr, in complex with its cognate signaling peptide papr, is the main regulator of virulence in b. ce ... | 2015 | 26779156 |
| antimicrobial effects of marine algal extracts and cyanobacterial pure compounds against five foodborne pathogens. | the marine environment is a proven source of structurally complex and biologically active compounds. in this study, the antimicrobial effects of a small collection of marine-derived extracts and isolates, were evaluated against 5 foodborne pathogens using a broth dilution assay. results demonstrated that algal extracts from padina and ulva species and cyanobacterial compounds antillatoxin b, laxaphycins a, b and b3, isomalyngamide a, and malyngamides c, i and j showed antimicrobial activity agai ... | 2016 | 26775951 |
| genomic characterization and comparison of seven myoviridae bacteriophage infecting bacillus thuringiensis. | bacillus thuringiensis kurstaki, a bacterium that is a source of biopesticides and a safe simulant for pathogenic bacillus species, was used to isolate seven unique bacteriophages. the phage genomes were sequenced and ranged in size from 158,100 to 163,019 bp encoding 290-299 genes, and the gc content of ~38% was similar to that of the host bacterium. all phages had terminal repeats 2-3 kb long. three of the phages encoded trnas and three contained a self-splicing intron in the dna polymerase ge ... | 2016 | 26773385 |
| adaptive responses of bacillus cereus atcc14579 cells upon exposure to acid conditions involve atpase activity to maintain their internal ph. | | 2015 | 26769448 |
| butanol production under microaerobic conditions with a symbiotic system of clostridium acetobutylicum and bacillus cereus. | one major problem of abe (acetone, butanol and ethanol) fermentation is high oxygen sensitivity of clostridium acetobutylicum. currently, no single strain has been isolated or genetically engineered to produce butanol effectively under aerobic conditions. in our previous work, a symbiotic system tsh06 has been developed successfully by our group, and two strains, c. acetobutylicum tsh1 and bacillus cereus tsh2, were isolated from tsh06. | 2016 | 26762531 |
| microbiology of corneal wounds after cataract surgery: biofilm formation and antibiotic resistance patterns. | to evaluate the bacterial flora of corneal wounds at the end of cataract surgery before intracameral antibiotic use and to determine agents to treat postoperative endophthalmitis, the potential for biofilm formation, and antibiotic resistance. | 2016 | 26762493 |
| antimicrobial effect of nisin against bacillus cereus in beef jerky during storage. | the microbial distribution of raw materials and beef jerky, and the effect of nisin on the growth of bacillus cereus inoculated in beef jerky during storage, were studied. five strains of pathogenic b. cereus were detected in beef jerky, and identified with 99.8% agreement using api chb 50 kit. to evaluate the effect of nisin, beef jerky was inoculated with approximately 3 log cfu/g of b. cereus mixed culture and nisin (100 iu/g and 500 iu/g). during the storage of beef jerky without nisin, the ... | 2015 | 26761838 |
| rapid detection of cronobacter sakazakii by real-time pcr based on the cgca gene and taqman probe with internal amplification control. | cronobacter sakazakii is a severe virulent strain that is frequently detected in powdered infant formula (pif). therefore, it is necessary to develop a fast and specific detection method. the specificity of our newly developed quantitative real-time pcr (qrt-pcr) was validated with dna from 46 strains. among them, 12 c. sakazakii strains were correctly amplified, whereas no positive florescent signal was observed from 34 nontarget controls. the detection limit of c. sakazakii was about 110 cfu/m ... | 2016 | 26751178 |
| advanced molecular diagnostic techniques for detection of food-borne pathogens; current applications and future challenges. | the elimination of disease-causing microbes from the food supply is a primary goal and this review deals with the overall techniques availavle for detection of food-borne pathogens. now-a-days conventional methods are replaced by advanced methods like biosensors, nucleic acid-based tests (nat) and different pcr based techniques used in molecular biology to identify specific pathogens. bacillus cereus, staphylococcus aureus, proteus vulgaris, escherichia coli, campylobacter, listeria monocytogene ... | 2016 | 26745757 |
| transcriptome analysis of bacillus thuringiensis spore life, germination and cell outgrowth in a vegetable-based food model. | toxigenic species belonging to bacillus cereus sensu lato, including bacillus thuringiensis, cause foodborne outbreaks thanks to their capacity to survive as spores and to grow in food matrixes. the goal of this work was to assess by means of a genome-wide transcriptional assay, in the food isolate b. thuringiensis uc10070, the gene expression behind the process of spore germination and consequent outgrowth in a vegetable-based food model. scanning electron microscopy and energy dispersive x-ray ... | 2016 | 26742618 |
| safety of street-vended soy wara in nigeria. | soy wara is a common ready-to-eat food whose production and sale are currently unregulated. microbiological sampling indicated that 21% of the samples had standard plate counts exceeding 100,000 cfu/g, and 14% had staphylococcus aureus counts higher than 100,000 cfu/g. the occurrence of s. aureus at these levels can result in food poisoning. listeria monocytogenes was isolated in 14.4% of the samples, although the counts were generally low, typically <1,000 cfu/g. although counts of l. monocytog ... | 2016 | 26735046 |
| microbiological analysis of rice cake processing in korea. | this study was conducted to evaluate the microbial contamination in rice cake materials and products during processing and in the operation environment in nonhazard analysis [and] critical control point factories. furthermore, the environmental health of the processing facilities and the bacterial and fungal contamination on the workers' hands were investigated. pour plate methods were used for enumeration of aerobic plate count (apc), yeast and molds (ym), bacillus cereus, staphylococcus aureus ... | 2016 | 26735044 |
| synthesis and properties of cellulose-functionalized poss-sio2/tio2 hybrid composites. | the mechanical, thermal, optical, electrical and morphological properties of cellulose, an excellent natural biomaterial, can be improved by organic-inorganic hybrid composite methods. based on the pristine properties of cellulose, the preparation of cellulose-metal oxide hybrid nanocomposites using a dispersion process of nanoparticles into the cellulose host matrix by traditional methods, has limitations. recently, the functionalized cellulose-polymer-based materials were considered to be an i ... | 2015 | 26726461 |
| antibacterial effects of alchornea cordifolia (schumach. and thonn.) müll. arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. | the leaves, stems and roots of alchornea cordifolia (schumach. and thonn.) müll. arg. are used as traditional medicine in many african countries for the management of gastrointestinal, respiratory and urinary tract infections as well as for the treatment of wounds. | 2016 | 26724423 |
| effects of potential probiotic bacillus cereus en25 on growth, immunity and disease resistance of juvenile sea cucumber apostichopus japonicus. | this study was conducted to determine effects of potential probiotic bacillus cereus en25 (isolated from mud of sea cucumber culturing water bodies) on growth, immunity and disease resistance against vibrio splendidus infection in juvenile sea cucumbers apostichopus japonicus. animals were respectively fed diets with b. cereus en25 at 0 (control), 10(5), 10(7) and 10(9) cfu/g for 30 days. results showed that dietary b. cereus en25 had no significant effects on growth, total coelomocytes counts a ... | 2016 | 26723266 |
| evaluation of anti-bacterial effects of some novel thiazole and imidazole derivatives against some pathogenic bacteria. | bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bacterial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus, listeria monocytogenes, escherichia coli, salmonella typhimurium, proteus mirabilis and shigella dysenteriae. | 2015 | 26719785 |
| synthesis of sic/ag/cellulose nanocomposite and its antibacterial activity by reactive oxygen species generation. | we describe the synthesis of nanocomposites, based on nanofibers of silicon carbide, silver nanoparticles, and cellulose. silver nanoparticle synthesis was achieved with chemical reduction using hydrazine by adding two different surfactants to obtain a nanocomposite with silver nanoparticles of different diameters. determination of antibacterial activity was based on respiration tests. enzymatic analysis indicates oxidative stress, and viability testing was conducted using an epifluorescence mic ... | 2016 | 28335299 |
| bio-prospecting of cuttle fish waste and cow dung for the production of fibrinolytic enzyme from bacillus cereus ind5 in solid state fermentation. | the process parameters governing the production of fibrinolytic enzyme in solid state fermentation employing bacillus cereus ind5 and using cuttle fish waste and cow dung substrate were optimized. the ph value of the medium, moisture content, sucrose, casein and magnesium sulfate were considered for two-level full factorial design and ph, casein and magnesium sulfate were identified as the important factors for fibrinolytic enzyme production. central composite design was applied to investigate t ... | 2016 | 28330303 |
| in vitro cytotoxicity and antibacterial activity of silver-coated electrospun polycaprolactone/gelatine nanofibrous scaffolds. | the development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. in this study, polycaprolactone (pcl) and pcl/gelatine (ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. nanofibrous scaffolds were coated with silver (ag) in different concentrations of silver nitrate (agno3) aqueous solution (1.25, 2.5, 5, and 10 %) by usi ... | 2016 | 28330282 |
| biodegradation of real petroleum wastewater by immobilized hyper phenol-tolerant strains of bacillus cereus in a fluidized bed bioreactor. | microbial bioremediation of petroleum wastewater by phenol-degrading-bacteria holds promise in circumventing the issue of petroleum-spill related pollution. herein, biodegradation of petroleum wastewater samples collected from oil refinery site was carried out in a fluidized bed bioreactor by ca-alginate immobilized biomass of phenol-degrading strains of bacillus cereus (akg1 mtcc9817 and akg2 mtcc9818). degradation performance of the system was evaluated by measuring the changes in chemical oxy ... | 2016 | 28330209 |
| isolation and characterization of bacterial endophytes of curcuma longa l. | fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ... | 2016 | 28330130 |
| production of γ-cyclodextrin by bacillus cereus cyclodextrin glycosyltransferase using extractive bioconversion in polymer-salt aqueous two-phase system. | aqueous two-phase system (atps) extractive bioconversion provides a technique which integrates bioconversion and purification into a single step process. extractive bioconversion of gamma-cyclodextrin (γ-cd) from soluble starch with cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) enzyme derived from bacillus cereus was evaluated using polyethylene glycol (peg)/potassium phosphate based on atps. the optimum condition was attained in the atps constituted of 30.0% (w/w) peg 3000 g/mol and 7. ... | 2016 | 26702953 |
| comparative elimination of dimethyl disulfide by maifanite and ceramic-packed biotrickling filters and their response to microbial community. | unpleasant odor emissions have traditionally occupied an important role in environmental concern. in this paper, twin biotrickling filters (btfs) packed with different packing materials, seeded with bacillus cereus gigan2, were successfully constructed to purify gaseous dimethyl disulfide (dmds). the maifanite-packed btf showed superior biodegradation capability to the ceramic-packed counterpart in terms of removal efficiency and elimination capacity under similar conditions. at an empty bed res ... | 2016 | 26702514 |
| biocatalytic resolution of rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester by immobilized recombinant bacillus cereus esterase. | a new esterase-producing strain (bacillus cereus wzz001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac-α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester (r, s-1) has been isolated from soil sample by our laboratory. in this study, the stereoselective hydrolysis of (r, s-1) was performed using the recombinant bacillus cereus esterase which expressed in escherichia coli bl21 (de3). under the optimized conditions of ph 8.0, 35 °c, and concentration of substrate 4 ... | 2016 | 26695776 |
| molecular characterization and biological effects of a c-type lectin-like receptor in large yellow croaker (larimichthys crocea). | the c-type lectin-like receptors (ctlrs) play important roles in innate immunity as one type of pattern recognition receptors. here, we cloned and characterized a c-type lectin-like receptor (lycctlr) from large yellow croaker larimichthys crocea. the full-length cdna of lycctlr is 880 nucleotides long, encoding a protein of 215 amino acids. the deduced lycctlr contains a c-terminal c-type lectin-like domain (ctld), an n-terminal cytoplasmic tail, and a transmembrane region. the ctld of lycctlr ... | 2015 | 26690423 |
| cost-effective-substrates for production of poly-β-hydroxybutyrate by a newly isolated bacillus cereus ps-10. | poly-β-hydroxybutyrate (phb) may serve as one of the imperative substitutes for petroleum derived plastics because of their close functional analogy and biodegradation quality. in the present study, phb producing ability of bacterial isolates was examined on low-cost agro industrial residues. isolate ps-10 from domestic waste landfills, identified as bacillus cereus ps-10 produced and accumulated appreciable amount of phb. bacillus cereus ps-10 was capable of using a wide variety of agro-based r ... | 2015 | 26688964 |
| detection of viable enterotoxin-producing bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex pcr. | bacillus cereus is responsible for several outbreaks of foodborne diseases due to its emetic toxin and enterotoxin. enterotoxins, cytotoxin k (cytk), nonhemolytic enterotoxin (nhe), and hemolysin bl (hbl), have been recorded in several diarrheal cases due to food poisoning from b. cereus. the objective of this study was to develop a rapid and accurate method that combines multiplex pcr with propidium monoazide to selectively detect viable cells of enterotoxin-producing b. cereus in milk powder, ... | 2016 | 26686715 |
| next-generation whole-genome sequencing of eight strains of bacillus cereus, isolated from food. | bacillus cereus can contaminate food and cause emetic and diarrheal foodborne illness. here, we report whole-genome sequences of eight strains of b. cereus, isolated from different food sources. | 2015 | 26679589 |
| subset selection of high-depth next generation sequencing reads for de novo genome assembly using mapreduce framework. | recent progress in next-generation sequencing technology has afforded several improvements such as ultra-high throughput at low cost, very high read quality, and substantially increased sequencing depth. state-of-the-art high-throughput sequencers, such as the illumina miseq system, can generate ~15 gbp sequencing data per run, with >80% bases above q30 and a sequencing depth of up to several 1000x for small genomes. illumina hiseq 2500 is capable of generating up to 1 tbp per run, with >80% bas ... | 2015 | 26678408 |
| the effects of citrus extract (citrox©) on the naturally occurring microflora and inoculated pathogens, bacillus cereus and salmonella enterica, in a model food system and the traditional greek yogurt-based salad tzatziki. | the antimicrobial effect of citrus extract (at 1 ml/kg [tc1] and 2 ml/kg [tc2]) on the naturally occurring microflora and inoculated pathogens (bacillus cereus and salmonella enterica, at ca. 6 log cfu/g) in the traditional greek yogurt-based salad tzatziki during storage under vacuum at 4 or 10 °c was examined. we also examined the effect of citrus extract (citrox(©)) against the two aforementioned pathogens in tryptic soy broth (tsb). of the two treatments, tc2 yielded the lowest yeast counts, ... | 2016 | 26678142 |
| lipase-catalyzed synthesis of palmitanilide: kinetic model and antimicrobial activity study. | enzymatic syntheses of fatty acid anilides are important owing to their wide range of industrial applications in detergents, shampoo, cosmetics, and surfactant formulations. the amidation reaction of mucor miehei lipase lipozyme im20 was investigated for direct amidation of triacylglycerol in organic solvents. the process parameters (reaction temperature, substrate molar ratio, enzyme amount) were optimized to achieve the highest yield of anilide. the maximum yield of palmitanilide (88.9%) was a ... | 2016 | 26672452 |
| structure elucidation, antimicrobial and cytotoxic activities of a halimane isolated from vellozia kolbekii alves (velloziaceae). | a new halimane diterpene was isolated from vellozia kolbekii alves (velloziaceae) and identified as (5r,8r,9s,13r)-halim-1,10-ene-15,16-diol (1). it showed cytotoxicity against three human cancer cell lines, sf-295 (glioblastoma), mda-mb-435 (melanoma), and hct-8 (colon adenocarcinoma). in the mechanism of cytotoxic action, halimane 1 interferes in two major phases of the cell cycle: in s phase, in which dna synthesis occurs and where it is very sensitive to damage, and g2m phase which is the ph ... | 2015 | 26663842 |
| genome sequence of a chromium-reducing strain, bacillus cereus s612. | we report here the genome sequence of an effective chromium-reducing bacterium, bacillus cereus strain s612. the size of the draft genome sequence is approximately 5.4 mb, with a g+c content of 35%, and it is predicted to contain 5,450 protein-coding genes. | 2015 | 26659672 |
| formation of small transmembrane pores: an intermediate stage on the way to bacillus cereus non-hemolytic enterotoxin (nhe) full pores in the absence of nhea. | the non-hemolytic enterotoxin (nhe) of bacillus cereus is a three-partite toxin formed of the components nhea, -b and -c. pore formation and subsequent lysis of target cells caused by nhe is an orchestrated process comprising three steps: (i) formation of nheb/c oligomers in solution, (ii) attachment of the oligomers to the cell membrane, (iii) binding of nhea to the oligomers. the present study aimed to characterize the properties of the nheb/c complex and the fate of the target cell upon bindi ... | 2016 | 26654951 |
| detection of multiple resistances, biofilm formation and conjugative transfer of bacillus cereus from contaminated soils. | the purpose of this study was to detect microbial resistances to a set of antibiotics/pesticides (multi-resistance) within pesticide and antibiotic-contaminated alluvial soils and to identify the corresponding antibiotic resistance genes (args). to assess whether identified multi-resistant isolates are able to construct biofilms, several biofilm formation and conjugation experiments were conducted. out of 35 isolates, six strains were used for filter mating experiments. nine strains were identif ... | 2016 | 26650381 |
| phytofabrication of bioinduced silver nanoparticles for biomedical applications. | synthesis of nanomaterials holds infinite possibilities as nanotechnology is revolutionizing the field of medicine by its myriad applications. green synthesis of nanoparticles has become the need of the hour because of its eco-friendly, nontoxic, and economic nature. in this study, leaf extract of rosa damascena was used as a bioreductant to reduce silver nitrate, leading to synthesis of silver nanoparticles (agnps) in a single step, without the use of any additional reducing or capping agents. ... | 2015 | 26648715 |
| the endospore-forming pathogen bacillus cereus exploits a small colony variant-based diversification strategy in response to aminoglycoside exposure. | bacillus cereus is among the microorganisms most often isolated from cases of food spoilage and causes gastrointestinal diseases as well as nongastrointestinal infections elicited by the emetic toxin cereulide, enterotoxins, and a panel of tissue-destructive virulence factors. this opportunistic pathogen is increasingly associated with rapidly fatal clinical infections especially linked to neonates and immunocompromised individuals. fatality results from either the misdiagnosis of b. cereus as a ... | 2015 | 26646008 |
| effects of electrolyzed oxidizing water on inactivation of bacillus subtilis and bacillus cereus spores in suspension and on carriers. | spores of some bacillus species are responsible for food spoilage and foodborne disease. these spores are highly resistant to various interventions and cooking processes. in this study, the sporicidal efficacy of acidic electrolyzed oxidizing (eo) water (aew) and slightly acidic eo water (saew) with available chlorine concentration (acc) of 40, 60, 80, 100, and 120 mg/l and treatment time for 1, 2, 3, 4, 5, and 6 min were tested on bacillus subtilis and bacillus cereus spores in suspension and o ... | 2016 | 26642381 |
| recovery and purification of chitosanase produced by bacillus cereus using expanded bed adsorption and central composite design. | this study presents a system for expanded bed adsorption for the purification of chitosanase from broth extract in a single step. a chitosanase-producing strain was isolated and identified as bacillus cereus c-01 and used to produce chitosanases. the expanded bed adsorption conditions for chitosanase purification were optimized statistically using streamline(tm) deae and a homemade column (2.6 × 30.0 cm). dependent variables were defined by the quality criteria purification factor (p) and enzyme ... | 2016 | 26638991 |
| root associated bacillus sp. improves growth, yield and zinc translocation for basmati rice (oryza sativa) varieties. | plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of punjab (pakistan). out of 234 isolates, 27 were able to solubilize zinc (zn) from different zn ores like zinc phosphate [zn3 (po4)2], zinc carbonate (znco3) and z ... | 2015 | 26635754 |
| silver nanoparticles cause complications in pregnant mice. | silver nanoparticles (agnps) have attracted much interest and have been used for antibacterial, antifungal, anticancer, and antiangiogenic applications because of their unique properties. the increased usage of agnps leads to a potential hazard to human health. however, the potential effects of agnps on animal models are not clear. this study was designed to investigate the potential impact of agnps on pregnant mice. | 2015 | 26622177 |
| effect of seed pelleting with biocontrol agents on growth and colonisation of roots of mungbean by root-infecting fungi. | mungbean (vigna radiata (l.) wilczek) is a leguminous pulse crop that is a major source of proteins, vitamins and minerals. root-infecting fungi produce severe plant diseases like root rot, charcoal rot, damping-off and stem rot. the soil-borne pathogens can be controlled by chemicals, but these chemicals have several negative effects. use of microbial antagonist such as fungi and bacteria is a safe, effective and eco-friendly method for the control of many soil-borne pathogens. biological contr ... | 2016 | 26619828 |
| streptomyces strains producing mitochondriotoxic antimycin a found in cereal grains. | reasons for mammalian cell toxicity observed in barley and spring wheat grains were sought. streptomyces sp. isolates from wheat and barley produced heat-stable methanol-soluble substances which inhibited the motility of exposed porcine spermatozoa used as a toxicity indicator. several barley isolates produced antimycin a (2 to 5 ng/mg wet wt of biomass), a macrolide antibiotic known to block oxygen utilization in mitochondria. the antimycin-producing isolates were members of the streptomyces al ... | 2016 | 26619316 |
| bacillus cereus ar156 activates pamp-triggered immunity and induces a systemic acquired resistance through a npr1-and sa-dependent signaling pathway. | induced resistance responses play a potent role in plant defense system against pathogen attack. bacillus cereus ar156 is a plant growth promoting rhizobacterium (pgpr) that installs induced systemic resistance (isr) to pseudomonas syringae pv. tomato (pst) in arabidopsis. here, we show that ar156 leaf infiltration enhances disease resistance in arabidopsis through the activation of a systemic acquired resistance (sar). pr1 protein expression and reactive oxygen species (ros) burst are strongly ... | 2016 | 26616055 |
| application of a rapid knowledge synthesis and transfer approach to assess the microbial safety of low-moisture foods. | low-moisture foods (lmf) are increasingly implicated in outbreaks of foodborne illness, resulting in a significant public health burden. to inform the development of a new codex alimentarius code of hygienic practice for lmf, we applied a rapid knowledge synthesis and transfer approach to review global research on the burden of illness, prevalence, and interventions to control nine selected microbial hazards in eight categories of lmf. knowledge synthesis methods included an integrated scoping r ... | 2015 | 26613924 |
| changes in the microbial composition of microbrewed beer during the process in the actual manufacturing line. | this study investigated changes in the microbial composition of microbrewed beer during the manufacturing processes and identified potential microbial hazards, effective critical quality control points, and potential contamination routes. comprehensive quantitative (aerobic plate count, lactic acid bacteria, fungi, acetic acid bacteria, coliforms, and bacillus cereus) and qualitative (escherichia coli and eight foodborne pathogens) microbiological analyses were performed using samples of raw mat ... | 2015 | 26613919 |
| dual-recognition detection of staphylococcus aureus using vancomycin-functionalized magnetic beads as concentration carriers. | vancomycin, which has a strong antibacterial effect to gram-positive bacteria, was adopted as one molecular recognition agent for bacterial detection. magnetic beads (mbs) were functionalized with this antibiotic to effectively concentrate staphylococcus aureus (s. aureus). in addition, alkaline phosphatase (alp)-tagged rabbit immunoglobulin g (alp-igg) was used as the second recognition agent to improve the specificity based on the binding between the fc region of rabbit igg and protein a in th ... | 2016 | 26606309 |
| production of rhamnolipids and diesel oil degradation by bacteria isolated from soil contaminated by petroleum. | biosurfactants are microbial secondary metabolites. the most studied are rhamnolipids, which decrease the surface tension and have emulsifying capacity. in this study, the production of biosurfactants, with emphasis on rhamnolipids, and diesel oil degradation by 18 strains of bacteria isolated from waste landfill soil contaminated by petroleum was analyzed. among the studied bacteria, gram-positive endospore forming rods (39%), gram positive rods without endospores (17%), and gram-negative rods ... | 2016 | 26588432 |
| erratum for erill and caruso, complete genome sequence of bacillus cereus group phage tsarbomba. | | 2015 | 26586903 |
| fibrinolytic enzyme production by newly isolated bacillus cereus srm-001 with enhanced in-vitro blood clot lysis potential. | the discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. herein, we report one such extra-cellular fibrinolytic enzyme producing bacillus cereus srm-001 isolated from the blood-laden soil of a chicken dump yard. the potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. the shake-flask operating parameters and media composition were optimized for maximizing the ... | 2015 | 26582284 |
| phenalenone derivatives and the unusual tricyclic sesterterpene acid from the marine fungus lophiostoma bipolare bcc25910. | ten compounds including nine phenalenone derivatives (five bipolarides and four bipolarols) and a sesterterpene acid (bipolarenic acid), were isolated from a marine isolated of the fungus lophiostoma bipolare (bcc25910), along with the known compounds, (-)-scleroderolide, (-)-sclerodin, and oxasetin. chemical structures were elucidated based on nmr spectroscopic data and hresims analysis. in addition, the absolute configurations of the phenalenones were resolved using specific rotations and chem ... | 2015 | 26582262 |
| microgravity-driven remodeling of the proteome reveals insights into molecular mechanisms and signal networks involved in response to the space flight environment. | space is a hostile environment characterized by high vacuum, extreme temperatures, meteoroids, space debris, ionospheric plasma, microgravity and space radiation, which all represent risks for human health. a deep understanding of the biological consequences of exposure to the space environment is required to design efficient countermeasures to minimize their negative impact on human health. recently, proteomic approaches have received a significant amount of attention in the effort to further s ... | 2016 | 26571091 |
| the mutation glu151asp in the b-component of the bacillus cereus non-hemolytic enterotoxin (nhe) leads to a diverging reactivity in antibody-based detection systems. | the ability of bacillus cereus to cause foodborne toxicoinfections leads to increasing concerns regarding consumer protection. for the diarrhea-associated enterotoxins, the assessment of the non-hemolytic enterotoxin b (nheb) titer determined by a sandwich enzyme immunoassay (eia) correlates best with in vitro cytotoxicity. in general, the regulation of enterotoxin expression of b. cereus is a coordinately-regulated process influenced by environmental, and probably also by host factors. as long ... | 2015 | 26569304 |
| design and synthesis of new 2-aryl-4,5-dihydro-thiazole analogues: in vitro antibacterial activities and preliminary mechanism of action. | sixty 2-aryl-4,5-dihydrothiazoles were designed and synthesized in yields ranging from 64% to 89% from cysteine and substituted-benzonitriles via a novel metal- and catalyst-free method. the structures of the title compounds were confirmed mainly by nmr spectral data analysis. antibacterial activity assays showed that the compounds (s)-2-(2'-hydroxyphenyl)-4-hydroxy-methyl- 4,5-dihydrothiazole (7h) and (r)-2-(2'-hydroxyphenyl)-4-hydroxymethyl-4,5-dihydro-thiazole (7h') exhibited significant inhi ... | 2015 | 26569197 |
| complete genome sequence of bacillus cereus forc_005, a food-borne pathogen from the soy sauce braised fish-cake with quail-egg. | due to abundant contamination in various foods, the pathogenesis of bacillus cereus has been widely studied in physiological and molecular level. b. cereus forc_005 was isolated from a korean side dish, soy sauce braised fish-cake with quail-egg in south korea. while 21 complete genome sequences of b. cereus has been announced to date, this strain was completely sequenced, analyzed, and compared with other complete genome sequences of b. cereus to elucidate the distinct pathogenic features of a ... | 2015 | 26566422 |
| antibacterial action against food-borne microorganisms and antioxidant activity of carvacrol-rich oil from lippia origanoides kunth. | lippia origanoides kunth from northeast brazil is a plant of pleasant odor used by local people as a food seasoning in substitution the oregano where its carvacrol-rich oil has showed significant antimicrobial activity against human pathogens. | 2015 | 26552874 |
| bacillus cereus adhesion to simulated intestinal mucus is determined by its growth on mucin, rather than intestinal environmental parameters. | adhesion of pathogenic bacteria to intestinal mucus, the protective layer of the gastrointestinal epithelium, is often considered a virulence factor. the ability of food-poisoning bacillus cereus strains to attach to mucus and the factors affecting this interaction have not yet been investigated. therefore, the role of adhesion in pathogenesis of b. cereus still remains unknown. in the present study, an in vitro assay based on mucin agar was used to simulate adhesion of b. cereus to mucus. bacte ... | 2015 | 26545170 |
| [screening of molluscacidal microorganisms against oncomelania hupensis and their effect]. | fifteen soil samples were collected from oncomelania hupensis culture pond in miluo schistosomiasis control and prevention base, hunan province. four strains of bacteria were identified to have molluscacidal effects, numbered as b8, b27, b36 and b59. compared with the fermentation broth groups and bacteria suspension groups, the fermentation supernatant groups of the four strains showed the strongest molluscacidal effect. the fermentation supernatant of b59 strain showed the best molluscacidal e ... | 2015 | 26541047 |
| comparison of primary models to predict microbial growth by the plate count and absorbance methods. | the selection of a primary model to describe microbial growth in predictive food microbiology often appears to be subjective. the objective of this research was to check the performance of different mathematical models in predicting growth parameters, both by absorbance and plate count methods. for this purpose, growth curves of three different microorganisms (bacillus cereus, listeria monocytogenes, and escherichia coli) grown under the same conditions, but with different initial concentrations ... | 2015 | 26539483 |
| identification of novel tylosin analogues generated by a wbla disruption mutant of streptomyces ansochromogenes. | streptomyces, as the main source of antibiotics, has been intensively exploited for discovering new drug candidates to combat the evolving pathogens. disruption of wbla, an actinobacteria-specific gene controlling major developmental transition, can cause the alteration of phenotype and morphology in many species of streptomyces. one wbla homologue was found in streptomyces ansochromogenes 7100 by using the basic local alignment search tool. it is interesting to identify whether novel secondary ... | 2015 | 26525981 |
| heavy metal detoxification by different bacillus species isolated from solar salterns. | the biosorption mechanism is an alternative for chemical precipitation and ultrafiltration which have been employed to treat heavy metal contamination with a limited success. in the present study, three species of bacillus which were isolated from solar salterns were screened for their detoxification potential of the heavy metals, lead, chromium, and copper, by biosorption. biosorption potential of each isolate was determined by atomic absorption spectroscopy (aas), inductively coupled plasma-op ... | 2015 | 26525498 |
| bacillus cereus infection: 57 case patients and a literature review. | we aimed to study the characteristics of patients presenting with a bacillus cereus infection in a university hospital. | 2016 | 26525185 |
| the dna glycosylase alkd uses a non-base-flipping mechanism to excise bulky lesions. | threats to genomic integrity arising from dna damage are mitigated by dna glycosylases, which initiate the base excision repair pathway by locating and excising aberrant nucleobases. how these enzymes find small modifications within the genome is a current area of intensive research. a hallmark of these and other dna repair enzymes is their use of base flipping to sequester modified nucleotides from the dna helix and into an active site pocket. consequently, base flipping is generally regarded a ... | 2015 | 26524531 |
| high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum. | enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ... | 2015 | 26519562 |
| production of co-polymers of polyhydroxyalkanoates by regulating the hydrolysis of biowastes. | production of polyhydroxyalkanoate (pha) co-polymers by bacillus spp. was studied by feeding defined volatile fatty acids (vfas) obtained through controlled hydrolysis of various wastes. eleven mixed hydrolytic cultures (mhcs) each containing 6 strains could generate vfa from slurries of (2% total solids): pea-shells (ps), potato peels (pp), apple pomace (ap) and onion peels (op). ps hydrolysates (obtained with mhc2 and mhc5) inoculated with bacillus cereus egu43 and bacillus thuringiensis egu45 ... | 2016 | 26512866 |
| microbiological quality of raw dried pasta from the german market, with special emphasis on cronobacter species. | the microbiological quality of 132 dried pasta products available on the german market, originating from 11 different countries, was studied. sample materials included soft or durum wheat products, some of which produced with other ingredients such as eggs, spices, or vegetables. parameters included hygiene indicators (aerobic plate count, mold count, the presence of enterobacteriaceae) and pathogenic/toxinogenic bacterial species (salmonella spp., staphylococcus aureus, presumptive bacillus cer ... | 2015 | 26509868 |
| role of plant growth promoting rhizobacteria and ag-nano particle in the bioremediation of heavy metals and maize growth under municipal wastewater irrigation. | the investigation evaluated the role of plant growth promoting rhizobacteria (pgpr) and ag-nano particle on the growth and metabolism of maize irrigated with municipal wastewater (mw). three pgpr isolated from mw were identified on the basis of 16s-rrna gene sequence analyses as pseudomonas sp., pseudomonas fluorescence, and bacillus cereus. the municipal waste water was used to irrigate the maize seeds inoculated with 3 isolated pgpr. the isolated pgpr had catalase and oxidase enzymes, solubili ... | 2016 | 26507686 |
| the c-terminal zwitterionic sequence of cotb1 is essential for biosilicification of the bacillus cereus spore coat. | silica is deposited in and around the spore coat layer of bacillus cereus, and enhances the spore's acid resistance. several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). homologous sequence search revealed a silacidin-like sequence in the c-terminal region of cotb1, a spore coat protein of b. cereus. the negatively charged silacidin-like sequence is followed by a positively charged arginine-rich ... | 2015 | 26503850 |
| phytochemical, sub-acute toxicity, and antibacterial evaluation of cordia sebestena leaf extracts. | in nigeria, cordia sebestena (boraginaceae), an understudied medicinal plant, is used in traditional medicine for the treatment of gastrointestinal disorders. in this study, we investigated the chemical composition, antibacterial potential, and sub-acute toxicity of c. sebestena leaves. | 2016 | 26501159 |
| proteomics identifies bacillus cereus entd as a pivotal protein for the production of numerous virulence factors. | bacillus cereus is a gram-positive pathogen that causes a wide variety of diseases in humans. it secretes into the extracellular milieu proteins that may contribute directly or indirectly to its virulence. entd is a novel exoprotein identified by proteogenomics of b. cereus atcc 14579. we constructed a δentd mutant and analyzed the impact of entd disruption on the cellular proteome and exoproteome isolated from early, late, and stationary-phase cultures. we identified 308 and 79 proteins regulat ... | 2015 | 26500610 |
| antioxidant, antibacterial activity, and phytochemical characterization of melaleuca cajuputi extract. | the threat posed by drug-resistant pathogens has resulted in the increasing momentum in research and development for effective alternative medications. the antioxidant and antibacterial properties of phytochemical extracts makes them attractive alternative complementary medicines. therefore, this study evaluated the phytochemical constituents of melaleuca cajuputi flower and leaf (gf and gl, respectively) extracts and their antioxidant and antibacterial activities. | 2015 | 26497742 |
| bacillus cereus nvh 0500/00 can adhere to mucin but cannot produce enterotoxins during gastrointestinal simulation. | adhesion to the intestinal epithelium could constitute an essential mechanism of bacillus cereus pathogenesis. however, the enterocytes are protected by mucus, a secretion composed mainly of mucin glycoproteins. these may serve as nutrients and sites of adhesion for intestinal bacteria. in this study, the food poisoning bacterium b. cereus nvh 0500/00 was exposed in vitro to gastrointestinal hurdles prior to evaluation of its attachment to mucin microcosms and its ability to produce nonhemolytic ... | 2015 | 26497468 |
| sporulation temperature reveals a requirement for cote in the assembly of both the coat and exosporium layers of bacillus cereus spores. | the bacillus cereus spore surface layers consist of a coat surrounded by an exosporium. we investigated the interplay between the sporulation temperature and the cote morphogenetic protein in the assembly of the surface layers of b. cereus atcc 14579 spores and on the resulting spore properties. the cote deletion affects the coat and exosporium composition of the spores formed both at the suboptimal temperature of 20°c and at the optimal growth temperature of 37°c. transmission electron microsco ... | 2015 | 26497467 |
| inactivation of bacillus cereus spores in a tsuyu sauce using continuous ohmic heating with five sequential elbow-type electrodes. | the effect of ohmic heating (oh) in a pilot plant system which had a zig-zag shaped (elbow-type) ohmic heater with five sequential voltage electrodes was investigated on bacillus cereus spores in a commercial tsuyu sauce. | 2016 | 26497155 |